AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38580.t1 MTR_3g099580 97.087 103 3 0 1 103 17 119 8.25e-71 209
MS.gene38580.t1 MTR_3g099570 86.408 103 14 0 1 103 17 119 2.49e-61 185
MS.gene38580.t1 MTR_3g099680 81.905 105 17 2 1 103 17 121 2.13e-54 169
MS.gene38580.t1 MTR_3g099980 81.905 105 17 2 1 103 17 121 1.60e-53 166
MS.gene38580.t1 MTR_3g099540 69.903 103 31 0 1 103 18 120 3.91e-47 147
MS.gene38580.t1 MTR_8g007035 58.491 106 41 2 1 103 14 119 1.21e-36 123
MS.gene38580.t1 MTR_8g007020 57.547 106 42 2 1 103 14 119 1.56e-35 120
MS.gene38580.t1 MTR_1g090190 44.792 96 53 0 7 102 20 115 7.06e-28 100
MS.gene38580.t1 MTR_6g080660 47.872 94 49 0 10 103 28 121 7.36e-26 95.9
MS.gene38580.t1 MTR_4g114870 49.495 99 45 2 10 103 191 289 8.60e-26 98.6
MS.gene38580.t1 MTR_4g114870 47.525 101 48 2 8 103 27 127 1.49e-25 98.2
MS.gene38580.t1 MTR_2g088990 44.898 98 53 1 6 102 21 118 1.11e-25 95.9
MS.gene38580.t1 MTR_6g023760 44.898 98 53 1 6 102 22 119 3.84e-24 90.5
MS.gene38580.t1 MTR_5g006040 44.444 99 52 3 7 102 23 121 4.89e-24 90.5
MS.gene38580.t1 MTR_0334s0010 42.157 102 56 1 5 103 23 124 2.68e-23 89.0
MS.gene38580.t1 MTR_1g104800 42.424 99 54 3 7 102 22 120 1.08e-22 87.0
MS.gene38580.t1 MTR_1g112700 38.614 101 60 2 5 103 28 128 4.78e-22 85.5
MS.gene38580.t1 MTR_7g086140 43.434 99 54 2 6 102 22 120 2.14e-21 83.2
MS.gene38580.t1 MTR_7g086190 43.434 99 54 2 6 102 22 120 2.14e-21 83.2
MS.gene38580.t1 MTR_8g099220 41.584 101 55 2 7 103 26 126 2.48e-20 79.3
MS.gene38580.t1 MTR_4g067200 38.182 110 61 3 1 103 19 128 8.22e-20 79.3
MS.gene38580.t1 MTR_4g066110 38.182 110 61 3 1 103 19 128 8.22e-20 79.3
MS.gene38580.t1 MTR_7g086160 41.837 98 55 2 7 102 24 121 1.07e-19 79.0
MS.gene38580.t1 MTR_6g083240 41.905 105 54 3 5 102 22 126 1.35e-19 79.0
MS.gene38580.t1 MTR_2g043730 43.000 100 53 3 5 103 27 123 2.43e-19 77.0
MS.gene38580.t1 MTR_1g105130 39.394 99 58 2 6 102 21 119 2.53e-19 77.8
MS.gene38580.t1 MTR_1g105120 39.394 99 58 2 6 102 21 119 2.53e-19 77.8
MS.gene38580.t1 MTR_4g081100 42.708 96 50 2 12 102 34 129 1.40e-18 77.4
MS.gene38580.t1 MTR_2g025580 39.583 96 56 2 10 103 27 122 3.97e-18 75.1
MS.gene38580.t1 MTR_3g089035 43.299 97 51 3 7 102 30 123 5.90e-18 73.2
MS.gene38580.t1 MTR_7g086220 37.374 99 60 2 6 102 21 119 7.17e-18 73.9
MS.gene38580.t1 MTR_7g086100 39.785 93 55 1 11 102 27 119 1.44e-17 74.7
MS.gene38580.t1 MTR_7g086090 40.860 93 54 1 11 102 27 119 1.90e-17 74.7
MS.gene38580.t1 MTR_2g083250 41.837 98 50 2 12 102 28 125 2.55e-17 74.7
MS.gene38580.t1 MTR_7g086280 37.755 98 60 1 6 102 22 119 5.45e-17 72.0
MS.gene38580.t1 MTR_7g086230 37.755 98 60 1 6 102 22 119 5.81e-17 72.0
MS.gene38580.t1 MTR_4g007250 39.000 100 59 2 4 102 23 121 1.53e-16 70.9
MS.gene38580.t1 MTR_1g090420 34.653 101 62 2 7 103 25 125 4.21e-16 70.9
MS.gene38580.t1 MTR_6g022170 36.275 102 59 2 7 103 31 131 2.55e-15 67.4
MS.gene38580.t1 MTR_8g463180 38.298 94 55 2 12 102 26 119 2.73e-15 69.7
MS.gene38580.t1 MTR_4g111640 33.333 102 61 2 8 103 30 130 8.96e-15 66.6
MS.gene38580.t1 MTR_2g101300 36.275 102 59 2 7 103 24 124 3.31e-14 65.1
MS.gene38580.t1 MTR_6g013420 38.462 104 59 4 3 102 30 132 5.31e-14 64.3
MS.gene38580.t1 MTR_4g077787 32.353 102 62 2 8 103 30 130 7.13e-14 64.3
MS.gene38580.t1 MTR_8g088830 39.000 100 52 5 7 102 25 119 1.29e-13 62.0
MS.gene38580.t1 MTR_6g013200 37.234 94 55 3 12 102 37 129 2.78e-13 62.4
MS.gene38580.t1 MTR_4g112310 35.000 80 51 1 24 103 2 80 4.70e-13 60.8
MS.gene38580.t1 MTR_2g090575 40.217 92 52 2 12 100 27 118 6.06e-13 63.2
MS.gene38580.t1 MTR_1g014120 35.789 95 56 2 13 102 29 123 9.89e-13 61.2
MS.gene38580.t1 MTR_3g105930 34.737 95 57 2 13 102 31 125 1.88e-12 60.5
MS.gene38580.t1 MTR_2g090580 37.234 94 56 2 12 102 29 122 2.04e-12 60.5
MS.gene38580.t1 MTR_4g124280 33.663 101 62 2 7 102 22 122 3.29e-12 60.5
MS.gene38580.t1 MTR_4g130800 40.260 77 45 1 27 102 50 126 3.45e-12 60.8
MS.gene38580.t1 MTR_4g130800 40.260 77 45 1 27 102 51 127 3.84e-12 60.8
MS.gene38580.t1 MTR_4g130780 37.500 80 49 1 24 102 48 127 9.21e-12 58.5
MS.gene38580.t1 MTR_8g086360 38.542 96 52 3 12 102 26 119 5.68e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38580.t1 AT1G72230 72.941 85 23 0 18 102 31 115 3.79e-43 139
MS.gene38580.t1 AT1G22480 65.882 85 29 0 18 102 27 111 4.36e-35 118
MS.gene38580.t1 AT2G32300 48.000 100 51 1 5 103 21 120 3.13e-29 105
MS.gene38580.t1 AT2G32300 48.000 100 51 1 5 103 51 150 8.88e-29 105
MS.gene38580.t1 AT3G60270 46.465 99 53 0 5 103 21 119 1.10e-27 100
MS.gene38580.t1 AT3G27200 44.898 98 53 1 6 102 22 119 3.96e-26 95.5
MS.gene38580.t1 AT5G07475 44.330 97 54 0 7 103 27 123 4.22e-25 93.6
MS.gene38580.t1 AT2G26720 45.361 97 51 2 8 102 27 123 1.74e-24 92.4
MS.gene38580.t1 AT2G31050 47.423 97 49 2 8 102 27 123 3.84e-24 91.3
MS.gene38580.t1 AT2G44790 51.136 88 41 1 18 103 36 123 1.62e-23 89.7
MS.gene38580.t1 AT5G26330 44.444 99 52 2 7 102 21 119 3.60e-23 88.6
MS.gene38580.t1 AT3G17675 37.500 104 64 1 1 103 23 126 1.19e-20 80.5
MS.gene38580.t1 AT3G60280 42.268 97 56 0 6 102 20 116 2.60e-20 81.6
MS.gene38580.t1 AT5G20230 41.237 97 53 2 10 102 25 121 4.80e-16 70.1
MS.gene38580.t1 AT2G25060 40.816 98 52 3 11 102 32 129 7.14e-16 69.3
MS.gene38580.t1 AT1G64640 38.000 100 58 2 7 102 30 129 1.54e-15 68.6
MS.gene38580.t1 AT3G18590 36.893 103 58 2 7 103 24 125 4.13e-15 67.4
MS.gene38580.t1 AT5G57920 39.241 79 47 1 25 102 43 121 1.19e-14 66.2
MS.gene38580.t1 AT5G57920 39.241 79 47 1 25 102 42 120 1.97e-14 65.5
MS.gene38580.t1 AT5G57920 39.241 79 47 1 25 102 75 153 2.52e-14 65.9
MS.gene38580.t1 AT4G31840 38.776 98 54 3 11 102 28 125 2.67e-14 65.1
MS.gene38580.t1 AT1G45063 39.000 100 53 4 9 102 148 245 2.92e-14 66.6
MS.gene38580.t1 AT1G45063 33.663 101 60 3 7 102 26 124 9.32e-11 57.0
MS.gene38580.t1 AT1G45063 39.000 100 53 4 9 102 148 245 4.06e-14 66.6
MS.gene38580.t1 AT4G12880 36.364 99 59 3 7 102 26 123 1.32e-13 62.8
MS.gene38580.t1 AT5G53870 36.170 94 57 2 12 102 32 125 2.37e-13 64.7
MS.gene38580.t1 AT4G30590 36.709 79 49 1 25 102 48 126 2.47e-13 62.8
MS.gene38580.t1 AT2G02850 39.000 100 51 4 7 102 33 126 3.45e-13 61.2
MS.gene38580.t1 AT4G28365 35.789 95 58 2 12 103 31 125 4.72e-13 62.4
MS.gene38580.t1 AT5G15350 36.364 99 61 2 7 103 26 124 5.35e-13 61.6
MS.gene38580.t1 AT1G48940 37.113 97 54 3 13 103 29 124 1.05e-12 60.8
MS.gene38580.t1 AT4G32490 32.979 94 60 2 12 102 33 126 5.67e-12 59.7
MS.gene38580.t1 AT1G79800 35.052 97 57 2 12 103 36 131 1.18e-11 58.5
MS.gene38580.t1 AT3G53330 33.654 104 64 4 3 102 182 284 1.27e-11 59.7
MS.gene38580.t1 AT5G53870 36.170 94 57 2 12 102 32 125 1.35e-11 59.7
MS.gene38580.t1 AT4G01380 32.979 94 53 4 17 102 79 170 4.09e-11 57.4

Find 29 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCATATGTGCTGTTCC+TGG 0.349993 3.3:-82039205 MS.gene38580:CDS
GATAAATCAGGTTGGGCAAT+TGG 0.365257 3.3:-82039588 MS.gene38580:CDS
GACTATAAATCATGCACAAC+TGG 0.368543 3.3:-82039292 MS.gene38580:CDS
ACTATGACAGTGTTCAACTA+TGG 0.385966 3.3:-82039349 MS.gene38580:intron
TGGCATGAAACTTTCTGTTA+AGG 0.430118 3.3:-82039170 None:intergenic
TCCTGCTTTCTTGAGAGGGA+TGG 0.445617 3.3:+82039234 None:intergenic
ATGCCACCTGTGCAATGTCC+AGG 0.481287 3.3:+82039187 None:intergenic
GTGTTCCTGCTTTCTTGAGA+GGG 0.482705 3.3:+82039230 None:intergenic
TGTGTTCCTGCTTTCTTGAG+AGG 0.493116 3.3:+82039229 None:intergenic
TTTCTTGAGAGGGATGGTTG+TGG 0.493786 3.3:+82039240 None:intergenic
CACACCGTTGGAGATAAATC+AGG 0.494660 3.3:-82039600 MS.gene38580:CDS
GGCTAGTGACAAAACATTTG+CGG 0.495560 3.3:-82039547 MS.gene38580:CDS
TTTGCGGTTGGTGACAGTCT+TGG 0.500707 3.3:-82039531 MS.gene38580:intron
CGTTGGAGATAAATCAGGTT+GGG 0.514147 3.3:-82039595 MS.gene38580:CDS
CTTGCAACTGTCCACACCGT+TGG 0.529565 3.3:-82039612 MS.gene38580:CDS
ACCATCCCTCTCAAGAAAGC+AGG 0.564418 3.3:-82039235 MS.gene38580:CDS
GTTCCTGGACATTGCACAGG+TGG 0.574513 3.3:-82039190 MS.gene38580:CDS
CCAACCTGATTTATCTCCAA+CGG 0.588831 3.3:+82039596 None:intergenic
GTGTGGACAGTTGCAAGAGT+TGG 0.591396 3.3:+82039618 None:intergenic
AGTGACAAAACATTTGCGGT+TGG 0.594495 3.3:-82039543 MS.gene38580:CDS
GCTGTTCCTGGACATTGCAC+AGG 0.594776 3.3:-82039193 MS.gene38580:CDS
CTTTAACTTCATCCACTGTG+TGG 0.606822 3.3:+82039320 None:intergenic
ACAGTGTTCAACTATGGTGC+TGG 0.611527 3.3:-82039343 MS.gene38580:intron
TTGGTTCTGATTATAACACA+TGG 0.617869 3.3:-82039569 MS.gene38580:CDS
CCGTTGGAGATAAATCAGGT+TGG 0.633245 3.3:-82039596 MS.gene38580:CDS
TGGTTCTGATTATAACACAT+GGG 0.658992 3.3:-82039568 MS.gene38580:CDS
CTGATTTATCTCCAACGGTG+TGG 0.663800 3.3:+82039601 None:intergenic
TCAATTAGTACAGACAGTAG+TGG 0.678569 3.3:-82039265 MS.gene38580:CDS
CTATGGTGCTGGCCACACAG+TGG 0.772990 3.3:-82039332 MS.gene38580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTAGATTTGTAAATTG+TGG + chr3.3:82039420-82039439 None:intergenic 15.0%
!! TGGTTCTGATTATAACACAT+GGG - chr3.3:82039230-82039249 MS.gene38580:CDS 30.0%
!! TTGGTTCTGATTATAACACA+TGG - chr3.3:82039229-82039248 MS.gene38580:CDS 30.0%
ACTATGACAGTGTTCAACTA+TGG - chr3.3:82039449-82039468 MS.gene38580:intron 35.0%
GACTATAAATCATGCACAAC+TGG - chr3.3:82039506-82039525 MS.gene38580:intron 35.0%
TCAATTAGTACAGACAGTAG+TGG - chr3.3:82039533-82039552 MS.gene38580:CDS 35.0%
AGTGACAAAACATTTGCGGT+TGG - chr3.3:82039255-82039274 MS.gene38580:CDS 40.0%
CCAACCTGATTTATCTCCAA+CGG + chr3.3:82039205-82039224 None:intergenic 40.0%
CGTTGGAGATAAATCAGGTT+GGG - chr3.3:82039203-82039222 MS.gene38580:CDS 40.0%
GGCTAGTGACAAAACATTTG+CGG - chr3.3:82039251-82039270 MS.gene38580:CDS 40.0%
! CTTTAACTTCATCCACTGTG+TGG + chr3.3:82039481-82039500 None:intergenic 40.0%
! TACTTCATATGTGCTGTTCC+TGG - chr3.3:82039593-82039612 MS.gene38580:CDS 40.0%
!! GATAAATCAGGTTGGGCAAT+TGG - chr3.3:82039210-82039229 MS.gene38580:CDS 40.0%
ACAGTGTTCAACTATGGTGC+TGG - chr3.3:82039455-82039474 MS.gene38580:intron 45.0%
CACACCGTTGGAGATAAATC+AGG - chr3.3:82039198-82039217 MS.gene38580:CDS 45.0%
CCGTTGGAGATAAATCAGGT+TGG - chr3.3:82039202-82039221 MS.gene38580:CDS 45.0%
CTGATTTATCTCCAACGGTG+TGG + chr3.3:82039200-82039219 None:intergenic 45.0%
GTGTTCCTGCTTTCTTGAGA+GGG + chr3.3:82039571-82039590 None:intergenic 45.0%
TGTGTTCCTGCTTTCTTGAG+AGG + chr3.3:82039572-82039591 None:intergenic 45.0%
TTTCTTGAGAGGGATGGTTG+TGG + chr3.3:82039561-82039580 None:intergenic 45.0%
ACCATCCCTCTCAAGAAAGC+AGG - chr3.3:82039563-82039582 MS.gene38580:CDS 50.0%
GTGTGGACAGTTGCAAGAGT+TGG + chr3.3:82039183-82039202 None:intergenic 50.0%
TCCTGCTTTCTTGAGAGGGA+TGG + chr3.3:82039567-82039586 None:intergenic 50.0%
!! TTTGCGGTTGGTGACAGTCT+TGG - chr3.3:82039267-82039286 MS.gene38580:CDS 50.0%
ATGCCACCTGTGCAATGTCC+AGG + chr3.3:82039614-82039633 None:intergenic 55.0%
CTTGCAACTGTCCACACCGT+TGG - chr3.3:82039186-82039205 MS.gene38580:CDS 55.0%
GCTGTTCCTGGACATTGCAC+AGG - chr3.3:82039605-82039624 MS.gene38580:CDS 55.0%
GTTCCTGGACATTGCACAGG+TGG - chr3.3:82039608-82039627 MS.gene38580:CDS 55.0%
!! CTATGGTGCTGGCCACACAG+TGG - chr3.3:82039466-82039485 MS.gene38580:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 82039171 82039649 82039171 ID=MS.gene38580
chr3.3 mRNA 82039171 82039649 82039171 ID=MS.gene38580.t1;Parent=MS.gene38580
chr3.3 exon 82039532 82039649 82039532 ID=MS.gene38580.t1.exon1;Parent=MS.gene38580.t1
chr3.3 CDS 82039532 82039649 82039532 ID=cds.MS.gene38580.t1;Parent=MS.gene38580.t1
chr3.3 exon 82039171 82039361 82039171 ID=MS.gene38580.t1.exon2;Parent=MS.gene38580.t1
chr3.3 CDS 82039171 82039361 82039171 ID=cds.MS.gene38580.t1;Parent=MS.gene38580.t1
Gene Sequence

>MS.gene38580

ATGGCACTGCCAACTCTTGCAACTGTCCACACCGTTGGAGATAAATCAGGTTGGGCAATTGGTTCTGATTATAACACATGGGCTAGTGACAAAACATTTGCGGTTGGTGACAGTCTTGGTAAGTATTATTAATTATTTCTTTGTTAAACACAGCTTAATGAGAAGAAAGCATGTAAAGATTGTAAACTGAAAAATAGTTAATTGAAGTTTTGTCAATTTCATAATTCATTTAGTTTTATATTGCAACCACAATTTACAAATCTAATATATATAACTTTACTATGACAGTGTTCAACTATGGTGCTGGCCACACAGTGGATGAAGTTAAAGAAAGTGACTATAAATCATGCACAACTGGAAATTCAATTAGTACAGACAGTAGTGGTGCCACAACCATCCCTCTCAAGAAAGCAGGAACACATTACTTCATATGTGCTGTTCCTGGACATTGCACAGGTGGCATGAAACTTTCTGTTAAG

Protein sequence

>MS.gene38580.t1

MALPTLATVHTVGDKSGWAIGSDYNTWASDKTFAVGDSLVFNYGAGHTVDEVKESDYKSCTTGNSISTDSSGATTIPLKKAGTHYFICAVPGHCTGGMKLSVK