Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054968.t1 | XP_003602634.2 | 97.1 | 34 | 1 | 0 | 17 | 50 | 237 | 270 | 1.80E-10 | 74.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054968.t1 | G7JC36 | 97.1 | 34 | 1 | 0 | 17 | 50 | 212 | 245 | 1.3e-10 | 74.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049567 | MS.gene054968 | 0.801136 | 1.04E-48 | -1.69E-46 |
| MS.gene053094 | MS.gene054968 | 0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene05410 | MS.gene054968 | 0.804131 | 2.50E-49 | -1.69E-46 |
| MS.gene054968 | MS.gene058477 | 0.808628 | 2.82E-50 | -1.69E-46 |
| MS.gene054968 | MS.gene070275 | 0.805791 | 1.13E-49 | -1.69E-46 |
| MS.gene054968 | MS.gene071551 | 0.814488 | 1.50E-51 | -1.69E-46 |
| MS.gene054968 | MS.gene073306 | 0.80108 | 1.07E-48 | -1.69E-46 |
| MS.gene054968 | MS.gene073428 | 0.809747 | 1.62E-50 | -1.69E-46 |
| MS.gene054968 | MS.gene28156 | 0.801138 | 1.04E-48 | -1.69E-46 |
| MS.gene054968 | MS.gene33109 | 0.81005 | 1.40E-50 | -1.69E-46 |
| MS.gene054968 | MS.gene40121 | 0.816879 | 4.40E-52 | -1.69E-46 |
| MS.gene054968 | MS.gene40123 | 0.823689 | 1.21E-53 | -1.69E-46 |
| MS.gene054968 | MS.gene58653 | 0.830968 | 2.18E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054968.t1 | MTR_3g096450 | 97.059 | 34 | 1 | 0 | 17 | 50 | 212 | 245 | 3.67e-19 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 6 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTCTTATATGATATAAA+AGG | 0.230401 | 3.1:+76746147 | MS.gene054968:CDS |
| CGCAGCTTCTCACTTTCAGA+AGG | 0.475966 | 3.1:+76746117 | MS.gene054968:CDS |
| TAAAGAAACTAGGAGGAGGA+TGG | 0.495341 | 3.1:+76746175 | MS.gene054968:CDS |
| AAAGGCAAAGTAAAGAAACT+AGG | 0.590890 | 3.1:+76746165 | MS.gene054968:CDS |
| AAAGTAAAGAAACTAGGAGG+AGG | 0.608499 | 3.1:+76746171 | MS.gene054968:CDS |
| GGCAAAGTAAAGAAACTAGG+AGG | 0.632445 | 3.1:+76746168 | MS.gene054968:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTAATGATATTTTTTAATTC+TGG | + | chr3.1:76746075-76746094 | MS.gene054968:CDS | 15.0% |
| !!! | CTTTTATATCATATAAGAAT+GGG | - | chr3.1:76746149-76746168 | None:intergenic | 15.0% |
| !! | CATGCATAAAAAATTTCAAT+TGG | + | chr3.1:76745999-76746018 | MS.gene054968:intron | 20.0% |
| !! | CCATTCTTATATGATATAAA+AGG | + | chr3.1:76746147-76746166 | MS.gene054968:CDS | 20.0% |
| !! | TAGAACAAAAAGATAAAGAA+TGG | + | chr3.1:76745701-76745720 | MS.gene054968:intron | 20.0% |
| !!! | CCTTTTATATCATATAAGAA+TGG | - | chr3.1:76746150-76746169 | None:intergenic | 20.0% |
| !!! | GTTATCTAATAAAGATTTTC+TGG | - | chr3.1:76745828-76745847 | None:intergenic | 20.0% |
| ! | AAGACTACTAAAGTACAAAA+TGG | + | chr3.1:76745670-76745689 | MS.gene054968:intron | 25.0% |
| !! | TTTATCTTTTTGTTCTACCT+CGG | - | chr3.1:76745698-76745717 | None:intergenic | 25.0% |
| !!! | TTTTTAATTCTGGTTTCAGT+AGG | + | chr3.1:76746085-76746104 | MS.gene054968:CDS | 25.0% |
| AAAGAACATCTTCAATAGGA+AGG | - | chr3.1:76745626-76745645 | None:intergenic | 30.0% | |
| AAAGGCAAAGTAAAGAAACT+AGG | + | chr3.1:76746165-76746184 | MS.gene054968:CDS | 30.0% | |
| AATGTTAAGAGTTGAAAGCA+TGG | + | chr3.1:76745591-76745610 | MS.gene054968:intron | 30.0% | |
| ATACATTCAATGTGAAGTGT+CGG | + | chr3.1:76745964-76745983 | MS.gene054968:intron | 30.0% | |
| TCTTTCCAATTTCTCTTGAT+TGG | + | chr3.1:76745641-76745660 | MS.gene054968:intron | 30.0% | |
| TTCATCCAATCAAGAGAAAT+TGG | - | chr3.1:76745649-76745668 | None:intergenic | 30.0% | |
| TTGGAAAGAACATCTTCAAT+AGG | - | chr3.1:76745630-76745649 | None:intergenic | 30.0% | |
| AAAGTAAAGAAACTAGGAGG+AGG | + | chr3.1:76746171-76746190 | MS.gene054968:CDS | 35.0% | |
| CATTTGTCAGTGTAACACAA+TGG | + | chr3.1:76745939-76745958 | MS.gene054968:intron | 35.0% | |
| GCAACAGCAATGATAAAAGC+AGG | + | chr3.1:76745785-76745804 | MS.gene054968:intron | 40.0% | |
| GGCAAAGTAAAGAAACTAGG+AGG | + | chr3.1:76746168-76746187 | MS.gene054968:CDS | 40.0% | |
| TAAAGAAACTAGGAGGAGGA+TGG | + | chr3.1:76746175-76746194 | MS.gene054968:CDS | 40.0% | |
| TAAAGTACAAAATGGCTCCG+AGG | + | chr3.1:76745678-76745697 | MS.gene054968:intron | 40.0% | |
| CGCAGCTTCTCACTTTCAGA+AGG | + | chr3.1:76746117-76746136 | MS.gene054968:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 76745584 | 76746206 | 76745584 | ID=MS.gene054968 |
| chr3.1 | mRNA | 76745584 | 76746206 | 76745584 | ID=MS.gene054968.t1;Parent=MS.gene054968 |
| chr3.1 | exon | 76745584 | 76745586 | 76745584 | ID=MS.gene054968.t1.exon1;Parent=MS.gene054968.t1 |
| chr3.1 | CDS | 76745584 | 76745586 | 76745584 | ID=cds.MS.gene054968.t1;Parent=MS.gene054968.t1 |
| chr3.1 | exon | 76746057 | 76746206 | 76746057 | ID=MS.gene054968.t1.exon2;Parent=MS.gene054968.t1 |
| chr3.1 | CDS | 76746057 | 76746206 | 76746057 | ID=cds.MS.gene054968.t1;Parent=MS.gene054968.t1 |
| Gene Sequence |
| Protein sequence |
