AlfalfaGEDB | MS.gene070275

M. sativa cultivar XinJiangDaYe / MS.gene070275

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070275.t1	XP_003610067.2	97	198	6	0	1	198	1	198	6.20E-83	317

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070275.t1	Q93Z27	37.0	200	108	3	6	198	16	204	5.2e-22	105.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070275.t1	G7JUB9	97.0	198	6	0	1	198	1	198	4.5e-83	317.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene070275	0.804156	2.47E-49	-1.69E-46
MS.gene049283	MS.gene070275	0.802502	5.44E-49	-1.69E-46
MS.gene049567	MS.gene070275	0.800041	1.74E-48	-1.69E-46
MS.gene049652	MS.gene070275	0.827124	1.86E-54	-1.69E-46
MS.gene04998	MS.gene070275	0.81533	9.77E-52	-1.69E-46
MS.gene050961	MS.gene070275	0.817105	3.92E-52	-1.69E-46
MS.gene051279	MS.gene070275	0.801406	9.14E-49	-1.69E-46
MS.gene051490	MS.gene070275	0.817577	3.07E-52	-1.69E-46
MS.gene051492	MS.gene070275	0.808635	2.81E-50	-1.69E-46
MS.gene051676	MS.gene070275	0.810354	1.20E-50	-1.69E-46
MS.gene051744	MS.gene070275	0.831333	1.77E-55	-1.69E-46
MS.gene051832	MS.gene070275	0.813303	2.74E-51	-1.69E-46
MS.gene052016	MS.gene070275	0.812545	4.02E-51	-1.69E-46
MS.gene052493	MS.gene070275	0.806886	6.62E-50	-1.69E-46
MS.gene052533	MS.gene070275	0.816005	6.91E-52	-1.69E-46
MS.gene052685	MS.gene070275	0.802265	6.09E-49	-1.69E-46
MS.gene053094	MS.gene070275	0.858317	8.95E-63	-1.69E-46
MS.gene053298	MS.gene070275	0.822391	2.43E-53	-1.69E-46
MS.gene053963	MS.gene070275	0.834623	2.69E-56	-1.69E-46
MS.gene05410	MS.gene070275	0.835714	1.43E-56	-1.69E-46
MS.gene05439	MS.gene070275	0.829094	6.24E-55	-1.69E-46
MS.gene054576	MS.gene070275	0.826936	2.06E-54	-1.69E-46
MS.gene054892	MS.gene070275	0.888487	5.66E-73	-1.69E-46
MS.gene054893	MS.gene070275	0.882195	1.28E-70	-1.69E-46
MS.gene054968	MS.gene070275	0.805791	1.13E-49	-1.69E-46
MS.gene055147	MS.gene070275	0.813397	2.61E-51	-1.69E-46
MS.gene055222	MS.gene070275	0.802652	5.07E-49	-1.69E-46
MS.gene055226	MS.gene070275	0.822084	2.86E-53	-1.69E-46
MS.gene055229	MS.gene070275	0.803086	4.12E-49	-1.69E-46
MS.gene055231	MS.gene070275	0.80637	8.50E-50	-1.69E-46
MS.gene055513	MS.gene070275	0.801054	1.08E-48	-1.69E-46
MS.gene056444	MS.gene070275	0.804029	2.63E-49	-1.69E-46
MS.gene056699	MS.gene070275	0.834192	3.45E-56	-1.69E-46
MS.gene05686	MS.gene070275	0.814268	1.68E-51	-1.69E-46
MS.gene057102	MS.gene070275	0.81643	5.55E-52	-1.69E-46
MS.gene057155	MS.gene070275	0.812483	4.14E-51	-1.69E-46
MS.gene057208	MS.gene070275	0.800667	1.29E-48	-1.69E-46
MS.gene057622	MS.gene070275	0.818892	1.55E-52	-1.69E-46
MS.gene058526	MS.gene070275	0.813181	2.91E-51	-1.69E-46
MS.gene058527	MS.gene070275	0.813027	3.15E-51	-1.69E-46
MS.gene058528	MS.gene070275	0.815119	1.09E-51	-1.69E-46
MS.gene058592	MS.gene070275	0.842792	2.08E-58	-1.69E-46
MS.gene05878	MS.gene070275	0.810218	1.29E-50	-1.69E-46
MS.gene059170	MS.gene070275	0.810914	9.09E-51	-1.69E-46
MS.gene05952	MS.gene070275	0.842944	1.90E-58	-1.69E-46
MS.gene059774	MS.gene070275	0.851265	9.93E-61	-1.69E-46
MS.gene05985	MS.gene070275	-0.826335	2.87E-54	-1.69E-46
MS.gene06023	MS.gene070275	0.8104	1.17E-50	-1.69E-46
MS.gene060259	MS.gene070275	0.81084	9.44E-51	-1.69E-46
MS.gene060373	MS.gene070275	0.800314	1.53E-48	-1.69E-46
MS.gene060412	MS.gene070275	0.842007	3.36E-58	-1.69E-46
MS.gene060413	MS.gene070275	0.811215	7.82E-51	-1.69E-46
MS.gene060520	MS.gene070275	0.802591	5.22E-49	-1.69E-46
MS.gene060697	MS.gene070275	0.84926	3.62E-60	-1.69E-46
MS.gene060840	MS.gene070275	0.816402	5.63E-52	-1.69E-46
MS.gene061047	MS.gene070275	0.879294	1.40E-69	-1.69E-46
MS.gene061188	MS.gene070275	0.816791	4.61E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070275.t1	MTR_4g127560	96.970	198	6	0	1	198	1	198	3.68e-139	387
MS.gene070275.t1	MTR_5g025690	63.874	191	61	2	8	198	18	200	5.11e-79	234
MS.gene070275.t1	MTR_7g011010	62.051	195	70	3	6	198	10	202	4.83e-70	212
MS.gene070275.t1	MTR_4g082050	47.458	118	57	4	81	197	29	142	7.75e-23	89.7
MS.gene070275.t1	MTR_7g074240	46.067	89	47	1	108	195	84	172	1.41e-16	74.3
MS.gene070275.t1	MTR_2g098890	29.744	195	116	4	19	194	11	203	1.39e-15	72.4
MS.gene070275.t1	MTR_2g023310	61.111	54	21	0	78	131	44	97	2.31e-14	66.2
MS.gene070275.t1	MTR_4g067270	47.761	67	35	0	129	195	77	143	7.95e-14	66.2
MS.gene070275.t1	MTR_4g115170	48.000	75	35	1	125	195	144	218	1.74e-12	64.3
MS.gene070275.t1	MTR_3g088655	41.053	95	51	1	99	193	65	154	3.08e-12	62.4
MS.gene070275.t1	MTR_3g109320	49.180	61	31	0	129	189	114	174	3.41e-12	62.4
MS.gene070275.t1	MTR_7g089760	50.820	61	30	0	134	194	82	142	3.82e-12	62.0
MS.gene070275.t1	MTR_8g107110	32.090	134	58	4	76	196	47	160	2.72e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070275.t1	AT5G39670	48.000	200	91	6	2	198	15	204	1.30e-47	155
MS.gene070275.t1	AT3G29000	59.459	111	43	1	87	197	85	193	1.69e-37	129
MS.gene070275.t1	AT4G20780	60.000	65	26	0	130	194	120	184	9.45e-20	82.8
MS.gene070275.t1	AT5G44460	54.545	66	30	0	130	195	111	176	5.80e-17	75.5
MS.gene070275.t1	AT3G59440	30.000	190	112	6	19	195	7	188	1.01e-16	75.1
MS.gene070275.t1	AT3G47480	48.101	79	39	2	120	197	105	182	1.23e-16	74.7
MS.gene070275.t1	AT2G43290	37.719	114	67	3	83	194	96	207	3.86e-16	73.9
MS.gene070275.t1	AT4G03290	53.125	64	30	0	131	194	82	145	1.74e-15	70.9
MS.gene070275.t1	AT1G05990	50.769	65	32	0	131	195	80	144	3.26e-15	70.1
MS.gene070275.t1	AT3G07490	51.613	62	30	0	134	195	82	143	2.04e-14	68.2
MS.gene070275.t1	AT4G12860	50.000	62	31	0	134	195	82	143	4.14e-13	64.3
MS.gene070275.t1	AT1G21550	31.298	131	66	3	84	193	25	152	2.51e-12	62.4

Find 23 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGAGAGTTCTTGTTATTT+TGG	0.121543	8.2:-5686468	MS.gene070275:CDS
AACTCAACTTCTCCCTTATT+TGG	0.181162	8.2:-5686881	MS.gene070275:CDS
CTTCTTTGCCAAATTCATTC+TGG	0.280945	8.2:-5686785	MS.gene070275:CDS
CTGAGCTACCAGAATGAATT+TGG	0.286669	8.2:+5686777	None:intergenic
TGAAGAGAATGAGCCTAGTT+TGG	0.350779	8.2:-5686558	MS.gene070275:CDS
GCAAATGTTGGCAGAGATAA+TGG	0.359399	8.2:-5686698	MS.gene070275:CDS
TGAAAGAGATGAAGTGAAAA+TGG	0.369654	8.2:-5686666	MS.gene070275:CDS
AAGGTCTATGAAACCAAATA+AGG	0.374441	8.2:+5686868	None:intergenic
CAGAATGAATTTGGCAAAGA+AGG	0.401758	8.2:+5686786	None:intergenic
GAGGAACTTGAAGAAAAGTA+TGG	0.414511	8.2:-5686605	MS.gene070275:CDS
CTATTACTATGTGAAATTTG+AGG	0.460627	8.2:+5686911	None:intergenic
AAACACTTCACAAAGCTCCT+TGG	0.468222	8.2:+5686580	None:intergenic
AGGTCTATGAAACCAAATAA+GGG	0.516554	8.2:+5686869	None:intergenic
AACAAACAAATCAGCAGAAG+TGG	0.517182	8.2:+5686336	None:intergenic
TGCTTCAACTCCTCCAAACT+AGG	0.518183	8.2:+5686545	None:intergenic
AGAGGTAAGAGATGAGAAGA+AGG	0.519907	8.2:-5686756	MS.gene070275:CDS
AGAGAATGAGCCTAGTTTGG+AGG	0.522617	8.2:-5686555	MS.gene070275:CDS
AAAATGTCAAGTATAGGAAA+AGG	0.526434	8.2:+5686849	None:intergenic
GTGTAGCAGTCAAGAGAATG+AGG	0.533790	8.2:-5686720	MS.gene070275:CDS
CAAGAGAATGAGGCAAATGT+TGG	0.535719	8.2:-5686710	MS.gene070275:CDS
TGAAGAAAAGTATGGTTCCA+AGG	0.559713	8.2:-5686597	MS.gene070275:CDS
AGATGAAGTGAAAATGGTGA+TGG	0.595216	8.2:-5686660	MS.gene070275:CDS
AATTCATTCTGGTAGCTCAG+AGG	0.688478	8.2:-5686774	MS.gene070275:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATACTTGAGAAAAAATTG+TGG	+	chr8.2:5686453-5686472	None:intergenic	20.0%
!!!	ACAATTTTTTCTCAAGTATA+TGG	-	chr8.2:5686452-5686471	MS.gene070275:CDS	20.0%
!!!	ATTTTTGATGAAAATCAAGA+TGG	-	chr8.2:5686868-5686887	MS.gene070275:CDS	20.0%
!	AAAATGTCAAGTATAGGAAA+AGG	+	chr8.2:5686420-5686439	None:intergenic	25.0%
!	CTATTACTATGTGAAATTTG+AGG	+	chr8.2:5686358-5686377	None:intergenic	25.0%
!	TGTTGAAAAATGTCAAGTAT+AGG	+	chr8.2:5686426-5686445	None:intergenic	25.0%
!!	CAAAAATTGTGATCATCTTT+TGG	+	chr8.2:5686855-5686874	None:intergenic	25.0%
!!!	AAAATGGTGATGGAAAAAAT+GGG	-	chr8.2:5686616-5686635	MS.gene070275:CDS	25.0%
	AAGGTCTATGAAACCAAATA+AGG	+	chr8.2:5686401-5686420	None:intergenic	30.0%
	AGGTCTATGAAACCAAATAA+GGG	+	chr8.2:5686400-5686419	None:intergenic	30.0%
	TGAAAGAGATGAAGTGAAAA+TGG	-	chr8.2:5686600-5686619	MS.gene070275:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	-	chr8.2:5686754-5686773	MS.gene070275:CDS	30.0%
!!	GAAAATGGTGATGGAAAAAA+TGG	-	chr8.2:5686615-5686634	MS.gene070275:CDS	30.0%
!!!	AGATGGTTTTATTGATGCAA+AGG	-	chr8.2:5686771-5686790	MS.gene070275:CDS	30.0%
!!!	CAGAGAGTTCTTGTTATTTT+GGG	-	chr8.2:5686799-5686818	MS.gene070275:CDS	30.0%
!!!	GTTATTTTGGGATTGAAACA+AGG	-	chr8.2:5686811-5686830	MS.gene070275:CDS	30.0%
	AACTCAACTTCTCCCTTATT+TGG	-	chr8.2:5686385-5686404	MS.gene070275:CDS	35.0%
	AGATGAAGTGAAAATGGTGA+TGG	-	chr8.2:5686606-5686625	MS.gene070275:CDS	35.0%
	CAGAATGAATTTGGCAAAGA+AGG	+	chr8.2:5686483-5686502	None:intergenic	35.0%
	CTTCTTTGCCAAATTCATTC+TGG	-	chr8.2:5686481-5686500	MS.gene070275:CDS	35.0%
	GAGGAACTTGAAGAAAAGTA+TGG	-	chr8.2:5686661-5686680	MS.gene070275:CDS	35.0%
!	TGAAGAAAAGTATGGTTCCA+AGG	-	chr8.2:5686669-5686688	MS.gene070275:CDS	35.0%
!!!	GCAGAGAGTTCTTGTTATTT+TGG	-	chr8.2:5686798-5686817	MS.gene070275:CDS	35.0%
	AAACACTTCACAAAGCTCCT+TGG	+	chr8.2:5686689-5686708	None:intergenic	40.0%
	AATTCATTCTGGTAGCTCAG+AGG	-	chr8.2:5686492-5686511	MS.gene070275:CDS	40.0%
	AGAGGTAAGAGATGAGAAGA+AGG	-	chr8.2:5686510-5686529	MS.gene070275:CDS	40.0%
	CAAGAGAATGAGGCAAATGT+TGG	-	chr8.2:5686556-5686575	MS.gene070275:CDS	40.0%
	CTGAGCTACCAGAATGAATT+TGG	+	chr8.2:5686492-5686511	None:intergenic	40.0%
	GCAAATGTTGGCAGAGATAA+TGG	-	chr8.2:5686568-5686587	MS.gene070275:CDS	40.0%
	TGAAGAGAATGAGCCTAGTT+TGG	-	chr8.2:5686708-5686727	MS.gene070275:CDS	40.0%
!	TTTTTGCAGCTCAGAAAGTG+AGG	-	chr8.2:5686642-5686661	MS.gene070275:CDS	40.0%
	AGAGAATGAGCCTAGTTTGG+AGG	-	chr8.2:5686711-5686730	MS.gene070275:CDS	45.0%
	GTGTAGCAGTCAAGAGAATG+AGG	-	chr8.2:5686546-5686565	MS.gene070275:CDS	45.0%
	TGCTTCAACTCCTCCAAACT+AGG	+	chr8.2:5686724-5686743	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	5686346	5686942	5686346	ID=MS.gene070275
chr8.2	mRNA	5686346	5686942	5686346	ID=MS.gene070275.t1;Parent=MS.gene070275
chr8.2	exon	5686346	5686942	5686346	ID=MS.gene070275.t1.exon1;Parent=MS.gene070275.t1
chr8.2	CDS	5686346	5686942	5686346	ID=cds.MS.gene070275.t1;Parent=MS.gene070275.t1

Gene Sequence

>MS.gene070275

ATGGCTCTTCCTCAAATTTCACATAGTAATAGCATTAGCAACTCAACTTCTCCCTTATTTGGTTTCATAGACCTTTTCCTATACTTGACATTTTTCAACAAAATCCACAATTTTTTCTCAAGTATATGGTTTTTCCTTCTTTGCCAAATTCATTCTGGTAGCTCAGAGGTAAGAGATGAGAAGAAGGTTTCAGAATCTAAGTGTAGCAGTCAAGAGAATGAGGCAAATGTTGGCAGAGATAATGGTTACATGATTGAAAGAGATGAAGTGAAAATGGTGATGGAAAAAATGGGATTTTTTTGCAGCTCAGAAAGTGAGGAACTTGAAGAAAAGTATGGTTCCAAGGAGCTTTGTGAAGTGTTTGAAGAGAATGAGCCTAGTTTGGAGGAGTTGAAGCAAGCTTTTGATGTTTTTGATGAGAACAAAGATGGTTTTATTGATGCAAAGGAATTGCAGAGAGTTCTTGTTATTTTGGGATTGAAACAAGGATCAGAATTTGAGAATTGCCAAAAGATGATCACAATTTTTGATGAAAATCAAGATGGAAGAATTGATTTCATTGAGTTTGTAAATATTATGAAAAACCACTTCTGCTGA

Protein sequence

>MS.gene070275.t1

MALPQISHSNSISNSTSPLFGFIDLFLYLTFFNKIHNFFSSIWFFLLCQIHSGSSEVRDEKKVSESKCSSQENEANVGRDNGYMIERDEVKMVMEKMGFFCSSESEELEEKYGSKELCEVFEENEPSLEELKQAFDVFDENKDGFIDAKELQRVLVILGLKQGSEFENCQKMITIFDENQDGRIDFIEFVNIMKNHFC