AlfalfaGEDB | MS.gene073306

M. sativa cultivar XinJiangDaYe / MS.gene073306

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073306.t1	XP_003610067.2	97	198	6	0	1	198	1	198	6.20E-83	317

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073306.t1	Q93Z27	37.0	200	108	3	6	198	16	204	5.2e-22	105.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073306.t1	G7JUB9	97.0	198	6	0	1	198	1	198	4.5e-83	317.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene073306	0.816203	6.24E-52	-1.69E-46
MS.gene049567	MS.gene073306	0.803357	3.62E-49	-1.69E-46
MS.gene049652	MS.gene073306	0.822436	2.37E-53	-1.69E-46
MS.gene050961	MS.gene073306	0.816842	4.49E-52	-1.69E-46
MS.gene051490	MS.gene073306	0.800317	1.53E-48	-1.69E-46
MS.gene051492	MS.gene073306	0.800798	1.22E-48	-1.69E-46
MS.gene051744	MS.gene073306	0.839491	1.54E-57	-1.69E-46
MS.gene052533	MS.gene073306	0.80472	1.89E-49	-1.69E-46
MS.gene053094	MS.gene073306	0.840163	1.03E-57	-1.69E-46
MS.gene053963	MS.gene073306	0.804312	2.30E-49	-1.69E-46
MS.gene05410	MS.gene073306	0.829671	4.52E-55	-1.69E-46
MS.gene05423	MS.gene073306	0.805286	1.44E-49	-1.69E-46
MS.gene05439	MS.gene073306	0.813667	2.28E-51	-1.69E-46
MS.gene054576	MS.gene073306	0.825742	3.97E-54	-1.69E-46
MS.gene054892	MS.gene073306	0.866037	3.82E-65	-1.69E-46
MS.gene054893	MS.gene073306	0.866206	3.38E-65	-1.69E-46
MS.gene054968	MS.gene073306	0.80108	1.07E-48	-1.69E-46
MS.gene055226	MS.gene073306	0.808397	3.16E-50	-1.69E-46
MS.gene055586	MS.gene073306	0.801017	1.10E-48	-1.69E-46
MS.gene056699	MS.gene073306	0.826831	2.19E-54	-1.69E-46
MS.gene05686	MS.gene073306	0.808629	2.82E-50	-1.69E-46
MS.gene057102	MS.gene073306	0.801358	9.35E-49	-1.69E-46
MS.gene057155	MS.gene073306	0.816427	5.56E-52	-1.69E-46
MS.gene057208	MS.gene073306	0.801515	8.68E-49	-1.69E-46
MS.gene058592	MS.gene073306	0.833651	4.72E-56	-1.69E-46
MS.gene05878	MS.gene073306	0.80473	1.88E-49	-1.69E-46
MS.gene05952	MS.gene073306	0.838582	2.64E-57	-1.69E-46
MS.gene059774	MS.gene073306	0.820941	5.26E-53	-1.69E-46
MS.gene05985	MS.gene073306	-0.825091	5.66E-54	-1.69E-46
MS.gene06023	MS.gene073306	0.810029	1.41E-50	-1.69E-46
MS.gene060412	MS.gene073306	0.832428	9.51E-56	-1.69E-46
MS.gene060697	MS.gene073306	0.819122	1.37E-52	-1.69E-46
MS.gene061047	MS.gene073306	0.859052	5.40E-63	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073306.t1	MTR_4g127560	96.970	198	6	0	1	198	1	198	3.68e-139	387
MS.gene073306.t1	MTR_5g025690	63.874	191	61	2	8	198	18	200	5.11e-79	234
MS.gene073306.t1	MTR_7g011010	62.051	195	70	3	6	198	10	202	4.83e-70	212
MS.gene073306.t1	MTR_4g082050	47.458	118	57	4	81	197	29	142	7.75e-23	89.7
MS.gene073306.t1	MTR_7g074240	46.067	89	47	1	108	195	84	172	1.41e-16	74.3
MS.gene073306.t1	MTR_2g098890	29.744	195	116	4	19	194	11	203	1.39e-15	72.4
MS.gene073306.t1	MTR_2g023310	61.111	54	21	0	78	131	44	97	2.31e-14	66.2
MS.gene073306.t1	MTR_4g067270	47.761	67	35	0	129	195	77	143	7.95e-14	66.2
MS.gene073306.t1	MTR_4g115170	48.000	75	35	1	125	195	144	218	1.74e-12	64.3
MS.gene073306.t1	MTR_3g088655	41.053	95	51	1	99	193	65	154	3.08e-12	62.4
MS.gene073306.t1	MTR_3g109320	49.180	61	31	0	129	189	114	174	3.41e-12	62.4
MS.gene073306.t1	MTR_7g089760	50.820	61	30	0	134	194	82	142	3.82e-12	62.0
MS.gene073306.t1	MTR_8g107110	32.090	134	58	4	76	196	47	160	2.72e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073306.t1	AT5G39670	48.000	200	91	6	2	198	15	204	1.30e-47	155
MS.gene073306.t1	AT3G29000	59.459	111	43	1	87	197	85	193	1.69e-37	129
MS.gene073306.t1	AT4G20780	60.000	65	26	0	130	194	120	184	9.45e-20	82.8
MS.gene073306.t1	AT5G44460	54.545	66	30	0	130	195	111	176	5.80e-17	75.5
MS.gene073306.t1	AT3G59440	30.000	190	112	6	19	195	7	188	1.01e-16	75.1
MS.gene073306.t1	AT3G47480	48.101	79	39	2	120	197	105	182	1.23e-16	74.7
MS.gene073306.t1	AT2G43290	37.719	114	67	3	83	194	96	207	3.86e-16	73.9
MS.gene073306.t1	AT4G03290	53.125	64	30	0	131	194	82	145	1.74e-15	70.9
MS.gene073306.t1	AT1G05990	50.769	65	32	0	131	195	80	144	3.26e-15	70.1
MS.gene073306.t1	AT3G07490	51.613	62	30	0	134	195	82	143	2.04e-14	68.2
MS.gene073306.t1	AT4G12860	50.000	62	31	0	134	195	82	143	4.14e-13	64.3
MS.gene073306.t1	AT1G21550	31.298	131	66	3	84	193	25	152	2.51e-12	62.4

Find 23 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGAGAGTTCTTGTTATTT+TGG	0.121543	8.1:+5294127	MS.gene073306:CDS
AACTCAACTTCTCCCTTATT+TGG	0.181162	8.1:+5293714	MS.gene073306:CDS
CTTCTTTGCCAAATTCATTC+TGG	0.280945	8.1:+5293810	MS.gene073306:CDS
CTGAGCTACCAGAATGAATT+TGG	0.286669	8.1:-5293818	None:intergenic
TGAAGAGAATGAGCCTAGTT+TGG	0.350779	8.1:+5294037	MS.gene073306:CDS
GCAAATGTTGGCAGAGATAA+TGG	0.359399	8.1:+5293897	MS.gene073306:CDS
TGAAAGAGATGAAGTGAAAA+TGG	0.369654	8.1:+5293929	MS.gene073306:CDS
AAGGTCTATGAAACCAAATA+AGG	0.374441	8.1:-5293727	None:intergenic
CAGAATGAATTTGGCAAAGA+AGG	0.401758	8.1:-5293809	None:intergenic
GAGGAACTTGAAGAAAAGTA+TGG	0.414511	8.1:+5293990	MS.gene073306:CDS
CTATTACTATGTGAAATTTG+AGG	0.460627	8.1:-5293684	None:intergenic
AAACACTTCACAAAGCTCCT+TGG	0.468222	8.1:-5294015	None:intergenic
AGGTCTATGAAACCAAATAA+GGG	0.516554	8.1:-5293726	None:intergenic
AACAAACAAATCAGCAGAAG+TGG	0.517182	8.1:-5294259	None:intergenic
TGCTTCAACTCCTCCAAACT+AGG	0.518183	8.1:-5294050	None:intergenic
AGAGGTAAGAGATGAGAAGA+AGG	0.519907	8.1:+5293839	MS.gene073306:CDS
AGAGAATGAGCCTAGTTTGG+AGG	0.522617	8.1:+5294040	MS.gene073306:CDS
AAAATGTCAAGTATAGGAAA+AGG	0.526434	8.1:-5293746	None:intergenic
GTGTAGCAGTCAAGAGAATG+AGG	0.533790	8.1:+5293875	MS.gene073306:CDS
CAAGAGAATGAGGCAAATGT+TGG	0.535719	8.1:+5293885	MS.gene073306:CDS
TGAAGAAAAGTATGGTTCCA+AGG	0.559713	8.1:+5293998	MS.gene073306:CDS
AGATGAAGTGAAAATGGTGA+TGG	0.595216	8.1:+5293935	MS.gene073306:CDS
AATTCATTCTGGTAGCTCAG+AGG	0.688478	8.1:+5293821	MS.gene073306:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATACTTGAGAAAAAATTG+TGG	-	chr8.1:5293782-5293801	None:intergenic	20.0%
!!!	ACAATTTTTTCTCAAGTATA+TGG	+	chr8.1:5293781-5293800	MS.gene073306:CDS	20.0%
!!!	ATTTTTGATGAAAATCAAGA+TGG	+	chr8.1:5294197-5294216	MS.gene073306:CDS	20.0%
!	AAAATGTCAAGTATAGGAAA+AGG	-	chr8.1:5293749-5293768	None:intergenic	25.0%
!	CTATTACTATGTGAAATTTG+AGG	-	chr8.1:5293687-5293706	None:intergenic	25.0%
!	TGTTGAAAAATGTCAAGTAT+AGG	-	chr8.1:5293755-5293774	None:intergenic	25.0%
!!	CAAAAATTGTGATCATCTTT+TGG	-	chr8.1:5294184-5294203	None:intergenic	25.0%
!!!	AAAATGGTGATGGAAAAAAT+GGG	+	chr8.1:5293945-5293964	MS.gene073306:CDS	25.0%
	AAGGTCTATGAAACCAAATA+AGG	-	chr8.1:5293730-5293749	None:intergenic	30.0%
	AGGTCTATGAAACCAAATAA+GGG	-	chr8.1:5293729-5293748	None:intergenic	30.0%
	TGAAAGAGATGAAGTGAAAA+TGG	+	chr8.1:5293929-5293948	MS.gene073306:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	+	chr8.1:5294083-5294102	MS.gene073306:CDS	30.0%
!!	GAAAATGGTGATGGAAAAAA+TGG	+	chr8.1:5293944-5293963	MS.gene073306:CDS	30.0%
!!!	AGATGGTTTTATTGATGCAA+AGG	+	chr8.1:5294100-5294119	MS.gene073306:CDS	30.0%
!!!	CAGAGAGTTCTTGTTATTTT+GGG	+	chr8.1:5294128-5294147	MS.gene073306:CDS	30.0%
!!!	GTTATTTTGGGATTGAAACA+AGG	+	chr8.1:5294140-5294159	MS.gene073306:CDS	30.0%
	AACTCAACTTCTCCCTTATT+TGG	+	chr8.1:5293714-5293733	MS.gene073306:CDS	35.0%
	AGATGAAGTGAAAATGGTGA+TGG	+	chr8.1:5293935-5293954	MS.gene073306:CDS	35.0%
	CAGAATGAATTTGGCAAAGA+AGG	-	chr8.1:5293812-5293831	None:intergenic	35.0%
	CTTCTTTGCCAAATTCATTC+TGG	+	chr8.1:5293810-5293829	MS.gene073306:CDS	35.0%
	GAGGAACTTGAAGAAAAGTA+TGG	+	chr8.1:5293990-5294009	MS.gene073306:CDS	35.0%
!	TGAAGAAAAGTATGGTTCCA+AGG	+	chr8.1:5293998-5294017	MS.gene073306:CDS	35.0%
!!!	GCAGAGAGTTCTTGTTATTT+TGG	+	chr8.1:5294127-5294146	MS.gene073306:CDS	35.0%
	AAACACTTCACAAAGCTCCT+TGG	-	chr8.1:5294018-5294037	None:intergenic	40.0%
	AATTCATTCTGGTAGCTCAG+AGG	+	chr8.1:5293821-5293840	MS.gene073306:CDS	40.0%
	AGAGGTAAGAGATGAGAAGA+AGG	+	chr8.1:5293839-5293858	MS.gene073306:CDS	40.0%
	CAAGAGAATGAGGCAAATGT+TGG	+	chr8.1:5293885-5293904	MS.gene073306:CDS	40.0%
	CTGAGCTACCAGAATGAATT+TGG	-	chr8.1:5293821-5293840	None:intergenic	40.0%
	GCAAATGTTGGCAGAGATAA+TGG	+	chr8.1:5293897-5293916	MS.gene073306:CDS	40.0%
	TGAAGAGAATGAGCCTAGTT+TGG	+	chr8.1:5294037-5294056	MS.gene073306:CDS	40.0%
!	TTTTTGCAGCTCAGAAAGTG+AGG	+	chr8.1:5293971-5293990	MS.gene073306:CDS	40.0%
	AGAGAATGAGCCTAGTTTGG+AGG	+	chr8.1:5294040-5294059	MS.gene073306:CDS	45.0%
	GTGTAGCAGTCAAGAGAATG+AGG	+	chr8.1:5293875-5293894	MS.gene073306:CDS	45.0%
	TGCTTCAACTCCTCCAAACT+AGG	-	chr8.1:5294053-5294072	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	5293675	5294271	5293675	ID=MS.gene073306
chr8.1	mRNA	5293675	5294271	5293675	ID=MS.gene073306.t1;Parent=MS.gene073306
chr8.1	exon	5293675	5294271	5293675	ID=MS.gene073306.t1.exon1;Parent=MS.gene073306.t1
chr8.1	CDS	5293675	5294271	5293675	ID=cds.MS.gene073306.t1;Parent=MS.gene073306.t1

Gene Sequence

>MS.gene073306

ATGGCTCTTCCTCAAATTTCACATAGTAATAGCATTAGCAACTCAACTTCTCCCTTATTTGGTTTCATAGACCTTTTCCTATACTTGACATTTTTCAACAAAATCCACAATTTTTTCTCAAGTATATGGTTTTTCCTTCTTTGCCAAATTCATTCTGGTAGCTCAGAGGTAAGAGATGAGAAGAAGGTTTCAGAATCTAAGTGTAGCAGTCAAGAGAATGAGGCAAATGTTGGCAGAGATAATGGTTACATGATTGAAAGAGATGAAGTGAAAATGGTGATGGAAAAAATGGGATTTTTTTGCAGCTCAGAAAGTGAGGAACTTGAAGAAAAGTATGGTTCCAAGGAGCTTTGTGAAGTGTTTGAAGAGAATGAGCCTAGTTTGGAGGAGTTGAAGCAAGCTTTTGATGTTTTTGATGAGAACAAAGATGGTTTTATTGATGCAAAGGAATTGCAGAGAGTTCTTGTTATTTTGGGATTGAAACAAGGATCAGAATTTGAGAACTGCCAAAAGATGATCACAATTTTTGATGAAAATCAAGATGGAAGAATTGATTTCATTGAGTTTGTAAATATTATGAAAAACCACTTCTGCTGA

Protein sequence

>MS.gene073306.t1

MALPQISHSNSISNSTSPLFGFIDLFLYLTFFNKIHNFFSSIWFFLLCQIHSGSSEVRDEKKVSESKCSSQENEANVGRDNGYMIERDEVKMVMEKMGFFCSSESEELEEKYGSKELCEVFEENEPSLEELKQAFDVFDENKDGFIDAKELQRVLVILGLKQGSEFENCQKMITIFDENQDGRIDFIEFVNIMKNHFC