AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055388

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055388.t1 MTR_8g006930 94.318 88 5 0 83 170 286 373 1.62e-53 174
MS.gene055388.t1 MTR_8g006930 96.512 86 3 0 1 86 1 86 6.57e-51 167
MS.gene055388.t1 MTR_7g056393 79.570 93 19 0 78 170 281 373 5.58e-48 159
MS.gene055388.t1 MTR_7g056393 77.907 86 19 0 1 86 1 86 1.17e-40 140
MS.gene055388.t1 MTR_4g119410 78.495 93 20 0 78 170 281 373 3.45e-46 155
MS.gene055388.t1 MTR_4g119410 76.744 86 20 0 1 86 1 86 1.26e-41 143
MS.gene055388.t1 MTR_7g078010 82.955 88 15 0 83 170 286 373 2.23e-45 153
MS.gene055388.t1 MTR_7g078010 72.917 96 24 1 1 96 1 94 2.08e-40 140
MS.gene055388.t1 MTR_7g056347 75.269 93 23 0 78 170 281 373 1.13e-43 148
MS.gene055388.t1 MTR_7g056347 67.442 86 28 0 1 86 1 86 3.09e-31 115
MS.gene055388.t1 MTR_7g078010 72.917 96 24 1 1 96 1 94 3.43e-41 140
MS.gene055388.t1 MTR_1g093110 58.095 105 38 2 70 170 272 374 3.65e-35 126
MS.gene055388.t1 MTR_1g093110 53.659 82 32 2 11 86 8 89 2.05e-20 86.7
MS.gene055388.t1 MTR_4g099390 64.706 85 29 1 86 170 1 84 3.94e-35 118
MS.gene055388.t1 MTR_1g093120 63.636 88 29 1 83 170 286 370 5.24e-34 123
MS.gene055388.t1 MTR_1g093120 52.564 78 33 2 13 86 9 86 2.17e-19 84.0
MS.gene055388.t1 MTR_1g093140 57.143 105 40 2 70 170 275 378 2.12e-33 121
MS.gene055388.t1 MTR_1g093140 54.762 84 29 2 12 86 9 92 2.43e-20 86.7
MS.gene055388.t1 MTR_8g469400 65.476 84 29 0 1 84 1 84 2.69e-31 115
MS.gene055388.t1 MTR_8g469400 54.545 88 40 0 83 170 273 360 1.94e-30 113
MS.gene055388.t1 MTR_4g024660 55.682 88 39 0 83 170 286 373 5.42e-31 115
MS.gene055388.t1 MTR_4g024660 65.116 86 30 0 1 86 1 86 6.14e-31 115
MS.gene055388.t1 MTR_8g062600 56.818 88 38 0 83 170 286 373 8.31e-31 114
MS.gene055388.t1 MTR_8g062600 59.302 86 35 0 1 86 1 86 7.44e-28 107
MS.gene055388.t1 MTR_2g069010 56.604 106 40 3 70 170 271 375 5.87e-30 112
MS.gene055388.t1 MTR_2g069010 52.778 72 32 1 17 86 17 88 5.37e-18 80.1
MS.gene055388.t1 MTR_8g063070 58.621 87 36 0 1 87 1 87 2.65e-29 110
MS.gene055388.t1 MTR_8g063070 55.914 93 41 0 78 170 281 373 5.10e-29 110
MS.gene055388.t1 MTR_2g069060 58.065 93 33 2 78 170 263 349 3.79e-29 110
MS.gene055388.t1 MTR_7g056353 60.920 87 34 0 83 169 260 346 4.08e-29 112
MS.gene055388.t1 MTR_7g056353 40.698 86 46 2 6 88 8 91 3.65e-11 60.8
MS.gene055388.t1 MTR_2g068960 48.819 127 51 4 49 170 252 369 4.01e-28 107
MS.gene055388.t1 MTR_2g068960 56.944 72 29 1 17 86 11 82 7.43e-20 85.1
MS.gene055388.t1 MTR_8g028435 54.839 93 39 1 78 170 275 364 4.94e-28 107
MS.gene055388.t1 MTR_8g009160 52.688 93 41 1 78 170 275 364 1.64e-27 105
MS.gene055388.t1 MTR_2g068950 48.031 127 52 4 49 170 252 369 1.72e-27 105
MS.gene055388.t1 MTR_2g068950 55.556 72 30 1 17 86 11 82 1.71e-18 81.6
MS.gene055388.t1 MTR_2g069090 60.227 88 32 1 83 170 288 372 1.79e-27 105
MS.gene055388.t1 MTR_2g069090 41.379 87 48 2 6 90 6 91 1.28e-11 62.0
MS.gene055388.t1 MTR_8g041600 53.763 93 41 1 78 170 284 374 3.08e-27 105
MS.gene055388.t1 MTR_8g041600 38.542 96 56 2 1 93 1 96 2.96e-12 63.9
MS.gene055388.t1 MTR_8g009110 43.200 125 58 3 49 170 251 365 6.25e-27 104
MS.gene055388.t1 MTR_2g069210 46.400 125 54 3 49 170 253 367 1.00e-26 103
MS.gene055388.t1 MTR_2g069210 45.349 86 43 3 10 93 7 90 1.14e-11 62.0
MS.gene055388.t1 MTR_8g009170 53.191 94 40 2 78 170 276 366 1.13e-26 103
MS.gene055388.t1 MTR_8g009090 53.763 93 40 1 78 170 275 364 2.90e-26 102
MS.gene055388.t1 MTR_8g009090 48.649 74 29 3 6 77 4 70 4.36e-11 60.5
MS.gene055388.t1 MTR_8g041620 50.538 93 44 1 78 170 284 374 1.26e-25 101
MS.gene055388.t1 MTR_8g041620 38.542 96 56 2 1 93 1 96 6.69e-13 65.9
MS.gene055388.t1 MTR_8g009080 42.400 125 59 3 49 170 283 397 1.97e-25 100
MS.gene055388.t1 MTR_8g009080 48.193 83 42 1 1 82 27 109 9.09e-16 73.9
MS.gene055388.t1 MTR_2g069190 54.839 93 39 1 78 170 282 371 2.08e-25 100
MS.gene055388.t1 MTR_2g069190 46.667 75 37 2 18 90 18 91 3.40e-11 60.8
MS.gene055388.t1 MTR_8g008930 54.545 88 37 1 83 170 281 365 3.27e-25 99.8
MS.gene055388.t1 MTR_8g008930 48.649 74 29 3 6 77 4 70 4.76e-11 60.5
MS.gene055388.t1 MTR_8g009180 52.273 88 39 1 78 165 205 289 1.24e-24 97.1
MS.gene055388.t1 MTR_8g009150 51.136 88 40 1 83 170 261 345 6.98e-24 95.9
MS.gene055388.t1 MTR_2g069300 51.042 96 38 2 78 170 269 358 1.30e-22 92.8
MS.gene055388.t1 MTR_2g069020 54.545 88 37 2 83 170 286 370 1.35e-22 92.8
MS.gene055388.t1 MTR_2g069020 58.333 72 28 1 17 86 15 86 2.19e-19 84.0
MS.gene055388.t1 MTR_2g069020 54.545 88 37 2 83 170 286 370 1.77e-22 92.8
MS.gene055388.t1 MTR_2g069020 58.333 72 28 1 17 86 15 86 2.73e-19 84.0
MS.gene055388.t1 MTR_8g008960 52.000 75 33 1 96 170 31 102 9.95e-22 84.7
MS.gene055388.t1 MTR_4g117880 38.732 142 72 3 28 165 230 360 1.02e-21 90.5
MS.gene055388.t1 MTR_8g009120 55.000 80 33 1 91 170 277 353 1.35e-21 89.7
MS.gene055388.t1 MTR_1g043770 39.683 126 61 3 49 170 241 355 1.59e-21 89.7
MS.gene055388.t1 MTR_1g043770 43.243 74 39 2 13 86 2 72 6.17e-11 60.1
MS.gene055388.t1 MTR_0032s0040 48.113 106 47 3 70 170 271 373 9.16e-21 87.8
MS.gene055388.t1 MTR_7g056370 58.333 60 25 0 27 86 6 65 1.29e-19 82.4
MS.gene055388.t1 MTR_7g029655 48.315 89 44 2 83 170 283 370 7.92e-19 82.4
MS.gene055388.t1 MTR_1g043810 45.783 83 42 1 83 165 273 352 2.79e-18 80.9
MS.gene055388.t1 MTR_1g043810 39.130 69 42 0 18 86 5 73 1.75e-11 61.6
MS.gene055388.t1 MTR_1g043775 44.578 83 43 1 83 165 273 352 5.96e-17 77.0
MS.gene055388.t1 MTR_1g070185 41.905 105 51 2 70 170 269 367 1.38e-16 76.3
MS.gene055388.t1 MTR_1g070185 42.308 78 42 2 17 93 18 93 7.37e-11 59.7
MS.gene055388.t1 MTR_8g009080 48.193 83 42 1 1 82 27 109 6.43e-16 73.9
MS.gene055388.t1 MTR_8g009080 48.193 83 42 1 1 82 27 109 6.93e-16 73.9
MS.gene055388.t1 MTR_1g043760 45.977 87 44 1 83 169 272 355 1.22e-15 73.6
MS.gene055388.t1 MTR_7g105070 39.326 89 50 2 83 170 277 362 5.83e-13 65.9
MS.gene055388.t1 MTR_8g041600 38.542 96 56 2 1 93 1 96 2.68e-12 63.5
MS.gene055388.t1 MTR_4g099380 45.714 70 31 2 19 86 124 188 6.95e-12 62.4
MS.gene055388.t1 MTR_2g069070 44.186 86 30 1 1 86 1 68 1.02e-11 60.8
MS.gene055388.t1 MTR_1g043810 39.130 69 42 0 18 86 5 73 1.41e-11 61.6
MS.gene055388.t1 MTR_1g043770 45.714 70 35 2 13 82 2 68 2.66e-11 60.8
MS.gene055388.t1 MTR_1g032140 40.909 88 46 3 83 169 282 364 3.24e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055388.t1 AT5G59540 57.000 100 36 2 70 165 265 361 5.92e-27 104
MS.gene055388.t1 AT5G59540 42.353 85 44 3 12 92 3 86 1.28e-11 62.0
MS.gene055388.t1 AT5G59530 55.682 88 36 1 83 170 280 364 7.38e-25 99.0
MS.gene055388.t1 AT5G59530 26.712 146 91 2 17 160 8 139 8.37e-11 59.7
MS.gene055388.t1 AT1G04380 53.409 88 38 1 83 170 261 345 1.11e-24 98.2
MS.gene055388.t1 AT1G06620 54.217 83 35 1 83 165 281 360 4.03e-22 91.7
MS.gene055388.t1 AT2G30840 42.857 126 57 5 49 170 248 362 5.94e-22 90.9
MS.gene055388.t1 AT2G30840 49.180 61 29 1 15 73 3 63 6.75e-13 65.9
MS.gene055388.t1 AT1G06650 51.685 89 39 2 83 170 284 369 1.34e-21 90.1
MS.gene055388.t1 AT1G06650 50.000 60 26 1 17 72 10 69 8.52e-11 59.7
MS.gene055388.t1 AT1G06645 51.685 89 39 2 83 170 294 379 1.49e-21 90.1
MS.gene055388.t1 AT1G06645 47.541 61 28 1 16 72 22 82 3.90e-11 60.8
MS.gene055388.t1 AT1G06640 51.685 89 39 2 83 170 284 369 8.96e-21 87.8
MS.gene055388.t1 AT1G06640 50.820 61 26 1 16 72 9 69 6.20e-12 63.2
MS.gene055388.t1 AT1G04350 53.933 89 37 2 83 170 275 360 1.96e-20 86.7
MS.gene055388.t1 AT5G43440 52.273 88 39 1 83 170 281 365 2.77e-20 86.3
MS.gene055388.t1 AT2G30830 46.602 103 43 3 69 170 267 358 1.45e-19 84.3
MS.gene055388.t1 AT2G30830 51.786 56 26 1 17 72 5 59 1.02e-11 62.4
MS.gene055388.t1 AT5G43450 56.627 83 33 1 83 165 278 357 2.87e-19 83.6
MS.gene055388.t1 AT2G30840 39.683 126 55 5 49 170 248 356 3.74e-17 77.8
MS.gene055388.t1 AT2G30840 49.180 61 29 1 15 73 3 63 6.90e-13 65.9
MS.gene055388.t1 AT2G25450 50.562 89 40 3 83 170 274 359 6.58e-17 77.0
MS.gene055388.t1 AT2G25450 45.763 59 32 0 17 75 5 63 3.23e-12 63.9
MS.gene055388.t1 AT3G61400 46.429 84 41 2 83 165 286 366 5.03e-16 74.7
MS.gene055388.t1 AT1G03410 44.304 79 40 2 83 160 277 352 3.58e-13 66.6
MS.gene055388.t1 AT1G03410 44.304 79 40 2 83 160 314 389 3.64e-13 66.6
MS.gene055388.t1 AT1G03400 47.500 80 38 2 83 161 295 371 3.68e-13 66.6
MS.gene055388.t1 AT2G30840 49.180 61 29 1 15 73 3 63 3.85e-13 66.2
MS.gene055388.t1 AT1G03400 46.250 80 39 2 83 161 267 343 7.94e-13 65.5
MS.gene055388.t1 AT1G06640 50.820 61 26 1 16 72 9 69 5.12e-12 62.8
MS.gene055388.t1 AT1G06640 50.820 61 26 1 16 72 9 69 5.44e-12 63.2
MS.gene055388.t1 AT5G59540 42.857 84 43 3 12 91 3 85 7.64e-12 62.4
MS.gene055388.t1 AT1G06650 50.000 60 26 1 17 72 10 69 9.37e-11 59.3

Find 41 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAAGGTTTGGTGGAGTC+TGG 0.223689 8.1:-86800242 MS.gene055388:CDS
CATTACCATAAAAGATTTCT+TGG 0.225535 8.1:-86798498 MS.gene055388:CDS
AAAGGTTTATGGTCCTATTA+AGG 0.275771 8.1:-86798558 MS.gene055388:CDS
TCTGAAAGCAGCTCCTTAAT+AGG 0.286985 8.1:+86798545 None:intergenic
GAAGGTACATCAAAGGTTTA+TGG 0.290841 8.1:-86798569 MS.gene055388:CDS
AATTAGTGGTTTCCTCTAAT+TGG 0.291896 8.1:+86800318 None:intergenic
AGATTTCCATCTTTCCAGTA+TGG 0.327504 8.1:+86800195 None:intergenic
ACCTTTGATGTACCTTCAAT+TGG 0.328992 8.1:+86798575 None:intergenic
GATTGTTGTGTATGTCTTTG+AGG 0.335557 8.1:+86800126 None:intergenic
TCAAAGGTTTGGTGGAGTCT+GGG 0.361579 8.1:-86800241 MS.gene055388:CDS
TCTATCATAAGTGGAATTAG+TGG 0.391072 8.1:+86800304 None:intergenic
ATGGTAATGTCTTTGTAGAT+TGG 0.407974 8.1:+86798512 None:intergenic
AAAAGTTGGTGTCAAAGGTT+TGG 0.421204 8.1:-86800252 MS.gene055388:CDS
TTGTTGAATTCACAACTTGA+AGG 0.434509 8.1:+86798430 None:intergenic
CCTTTGATGTACCTTCAATT+GGG 0.460323 8.1:+86798576 None:intergenic
ATTCCACGCATGTTCCATAC+TGG 0.461475 8.1:-86800209 MS.gene055388:CDS
TTCAACTTCTCTATCATAAG+TGG 0.471483 8.1:+86800295 None:intergenic
CCTTAATGGAAAATCTTCCT+TGG 0.479612 8.1:-86798456 MS.gene055388:CDS
AAGCTCGCTACGGAAATTCT+CGG 0.481237 8.1:+86798617 None:intergenic
AATTCCCGTGACCCAATTGA+AGG 0.494961 8.1:-86798587 MS.gene055388:CDS
GTAATGTCTTTGTAGATTGG+TGG 0.503861 8.1:+86798515 None:intergenic
CATGTTCCATACTGGAAAGA+TGG 0.504047 8.1:-86800201 MS.gene055388:CDS
GATTCAAAAGTTGGTGTCAA+AGG 0.506737 8.1:-86800257 MS.gene055388:CDS
TATGTCTTTGAGGTCTATGA+TGG 0.510313 8.1:+86800136 None:intergenic
TGTACCTTCAATTGGGTCAC+GGG 0.516559 8.1:+86798583 None:intergenic
TGTTGAATTCACAACTTGAA+GGG 0.521306 8.1:+86798431 None:intergenic
TAATGTCTTTGTAGATTGGT+GGG 0.528678 8.1:+86798516 None:intergenic
AGCTCGCTACGGAAATTCTC+GGG 0.535878 8.1:+86798618 None:intergenic
ATGTACCTTCAATTGGGTCA+CGG 0.539733 8.1:+86798582 None:intergenic
AGTTGGTGTCAAAGGTTTGG+TGG 0.541057 8.1:-86800249 MS.gene055388:CDS
CAAAGGTTTGGTGGAGTCTG+GGG 0.546548 8.1:-86800240 MS.gene055388:CDS
ATTACCTCTACACGTCGAGC+TGG 0.554369 8.1:+86800104 None:intergenic
CAATCCAGCTCGACGTGTAG+AGG 0.563713 8.1:-86800108 MS.gene055388:intron
CCCAATTGAAGGTACATCAA+AGG 0.564592 8.1:-86798576 MS.gene055388:CDS
TATGATGGGAATACTCAACT+TGG 0.571165 8.1:+86800151 None:intergenic
CTATGTGTTGTGCTAAGACT+CGG 0.578926 8.1:+86798642 None:intergenic
TCACAACTTGAAGGGCTCCA+AGG 0.582867 8.1:+86798439 None:intergenic
ATTCAGAAAGAAGCTCGCTA+CGG 0.628270 8.1:+86798607 None:intergenic
CGAGTCTTAGCACAACACAT+AGG 0.644734 8.1:-86798641 MS.gene055388:CDS
ATGTCTTTGAGGTCTATGAT+GGG 0.665783 8.1:+86800137 None:intergenic
TTTCCAGTATGGAACATGCG+TGG 0.671748 8.1:+86800206 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGCTTAAAAATATATTA+TGG - chr8.1:86799666-86799685 MS.gene055388:intron 10.0%
!!! TTTAATACTAAAATTTTTGT+TGG + chr8.1:86799526-86799545 None:intergenic 10.0%
!! CATATATAATCCATAAATAA+AGG + chr8.1:86799050-86799069 None:intergenic 15.0%
!! TAATGAACTCAATATTAATT+TGG - chr8.1:86798979-86798998 MS.gene055388:intron 15.0%
!! TATAATATTATCACATTCTA+AGG + chr8.1:86799971-86799990 None:intergenic 15.0%
!! TCAAATTTAATTGTATATCT+AGG - chr8.1:86799024-86799043 MS.gene055388:intron 15.0%
!! TCAAAACAATATTCAGTAAA+AGG - chr8.1:86799701-86799720 MS.gene055388:intron 20.0%
!! TCACAGAAAATAAAGAATTT+GGG - chr8.1:86799190-86799209 MS.gene055388:intron 20.0%
!! TGAATTATTATAGTCATTGA+TGG - chr8.1:86799129-86799148 MS.gene055388:intron 20.0%
!! TTCACAGAAAATAAAGAATT+TGG - chr8.1:86799189-86799208 MS.gene055388:intron 20.0%
!!! AACGATTTTGTTTTTAGAAA+TGG + chr8.1:86800019-86800038 None:intergenic 20.0%
!!! GAATATTGTTTTGAATCTTA+TGG + chr8.1:86799695-86799714 None:intergenic 20.0%
! AAGTTCTTCATGAAGATAAA+TGG - chr8.1:86799413-86799432 MS.gene055388:intron 25.0%
! AGTTCTTCATGAAGATAAAT+GGG - chr8.1:86799414-86799433 MS.gene055388:intron 25.0%
! CATTACCATAAAAGATTTCT+TGG - chr8.1:86800279-86800298 MS.gene055388:CDS 25.0%
! TAAATCTTATGGCATAGTAA+GGG + chr8.1:86799792-86799811 None:intergenic 25.0%
!! GGGTTTTACAAAAATAAATG+TGG + chr8.1:86799612-86799631 None:intergenic 25.0%
!! TATATCTAGGCCTTTATTTA+TGG - chr8.1:86799037-86799056 MS.gene055388:intron 25.0%
!!! CAAATTCTTTAAGGTAAGAA+GGG + chr8.1:86799258-86799277 None:intergenic 25.0%
!!! TAAAACCCCTAATTTTGAAA+AGG - chr8.1:86799624-86799643 MS.gene055388:intron 25.0%
!!! TAAGAAAAGAGTTTTATACG+CGG - chr8.1:86798821-86798840 MS.gene055388:intron 25.0%
AAACTTCATTCTTCAAGACT+AGG + chr8.1:86799570-86799589 None:intergenic 30.0%
AAAGGTTTATGGTCCTATTA+AGG - chr8.1:86800219-86800238 MS.gene055388:CDS 30.0%
AAAGTCTTAAAGTCATAGCA+TGG + chr8.1:86799840-86799859 None:intergenic 30.0%
AATTAGTGGTTTCCTCTAAT+TGG + chr8.1:86798462-86798481 None:intergenic 30.0%
ATAGATGACCAATATACAAG+TGG + chr8.1:86799909-86799928 None:intergenic 30.0%
ATATATGAATTCTCCTCTCA+TGG - chr8.1:86799063-86799082 MS.gene055388:intron 30.0%
ATTTCATCCAAGACATTAAC+GGG + chr8.1:86798760-86798779 None:intergenic 30.0%
CATTTCATCCAAGACATTAA+CGG + chr8.1:86798761-86798780 None:intergenic 30.0%
CCTTCTTACCTTAAAGAATT+TGG - chr8.1:86799256-86799275 MS.gene055388:intron 30.0%
CTTAAGAACCACTTGTATAT+TGG - chr8.1:86799898-86799917 MS.gene055388:intron 30.0%
GAAAAACACAAACCAATTAG+AGG - chr8.1:86798447-86798466 MS.gene055388:CDS 30.0%
GAGCATAGTTGTAAATCTTA+TGG + chr8.1:86799803-86799822 None:intergenic 30.0%
GTAAATCTTATGGCATAGTA+AGG + chr8.1:86799793-86799812 None:intergenic 30.0%
TAATGTCTTTGTAGATTGGT+GGG + chr8.1:86800264-86800283 None:intergenic 30.0%
TATCTTCATGAAGAACTTGA+AGG + chr8.1:86799411-86799430 None:intergenic 30.0%
TCTATCATAAGTGGAATTAG+TGG + chr8.1:86798476-86798495 None:intergenic 30.0%
TCTTCATCATTGAAAGTTTG+TGG - chr8.1:86800043-86800062 MS.gene055388:intron 30.0%
TTATAGTGAACTGCAATTGT+TGG - chr8.1:86799986-86800005 MS.gene055388:intron 30.0%
TTCAACTTCTCTATCATAAG+TGG + chr8.1:86798485-86798504 None:intergenic 30.0%
! ATGGTAATGTCTTTGTAGAT+TGG + chr8.1:86800268-86800287 None:intergenic 30.0%
! GTGATGCCTTTTCAAAATTA+GGG + chr8.1:86799633-86799652 None:intergenic 30.0%
! TCTAATGTACTCTTGTTTAG+TGG - chr8.1:86798871-86798890 MS.gene055388:intron 30.0%
! TGATGCCTTTTCAAAATTAG+GGG + chr8.1:86799632-86799651 None:intergenic 30.0%
! TGTGATGCCTTTTCAAAATT+AGG + chr8.1:86799634-86799653 None:intergenic 30.0%
!! AAGAAAAGAGTTTTATACGC+GGG - chr8.1:86798822-86798841 MS.gene055388:intron 30.0%
!! CCAAATTCTTTAAGGTAAGA+AGG + chr8.1:86799259-86799278 None:intergenic 30.0%
!! GCTTTTGATGATTCAAAAGT+TGG - chr8.1:86798511-86798530 MS.gene055388:CDS 30.0%
AAACATAGGAGATCTTCTAC+AGG - chr8.1:86799471-86799490 MS.gene055388:intron 35.0%
AATGTATCTCTCCAATTAGC+AGG + chr8.1:86798901-86798920 None:intergenic 35.0%
ACCCAAAGTAAATAGTCCTT+CGG + chr8.1:86799287-86799306 None:intergenic 35.0%
ACCTTTGATGTACCTTCAAT+TGG + chr8.1:86800205-86800224 None:intergenic 35.0%
AGATTTCCATCTTTCCAGTA+TGG + chr8.1:86798585-86798604 None:intergenic 35.0%
CCTGAACCTGAATTAACTAT+GGG - chr8.1:86799325-86799344 MS.gene055388:intron 35.0%
CCTTAATGGAAAATCTTCCT+TGG - chr8.1:86800321-86800340 MS.gene055388:CDS 35.0%
CCTTTGATGTACCTTCAATT+GGG + chr8.1:86800204-86800223 None:intergenic 35.0%
GATTGTTGTGTATGTCTTTG+AGG + chr8.1:86798654-86798673 None:intergenic 35.0%
GCTAATTGGAGAGATACATT+TGG - chr8.1:86798901-86798920 MS.gene055388:intron 35.0%
GTAATGTCTTTGTAGATTGG+TGG + chr8.1:86800265-86800284 None:intergenic 35.0%
GTCTTGGATGAAATGATTGA+TGG - chr8.1:86798766-86798785 MS.gene055388:intron 35.0%
TGAAATTCCCGTTAATGTCT+TGG - chr8.1:86798750-86798769 MS.gene055388:intron 35.0%
TGAGTTCATTACCTGCATAT+TGG + chr8.1:86798970-86798989 None:intergenic 35.0%
TTACTTCTACAAGACCAACT+TGG - chr8.1:86799382-86799401 MS.gene055388:intron 35.0%
TTAGTGGAAAACCTGCTAAT+TGG - chr8.1:86798887-86798906 MS.gene055388:intron 35.0%
TTTGATCATGATTCCATGAG+AGG + chr8.1:86799079-86799098 None:intergenic 35.0%
! ATGCGCCAAGAAATCTTTTA+TGG + chr8.1:86800287-86800306 None:intergenic 35.0%
! CCAAGGAAGATTTTCCATTA+AGG + chr8.1:86800324-86800343 None:intergenic 35.0%
! CCATAGTTAATTCAGGTTCA+GGG + chr8.1:86799327-86799346 None:intergenic 35.0%
! GAAGGTACATCAAAGGTTTA+TGG - chr8.1:86800208-86800227 MS.gene055388:CDS 35.0%
! TATGATGGGAATACTCAACT+TGG + chr8.1:86798629-86798648 None:intergenic 35.0%
! TTTTATGCAGAAGGCCTTAA+TGG - chr8.1:86800307-86800326 MS.gene055388:CDS 35.0%
!! AAAAGTTGGTGTCAAAGGTT+TGG - chr8.1:86798525-86798544 MS.gene055388:CDS 35.0%
!! AATCTTTGAGTTGTTGTCAG+AGG - chr8.1:86799219-86799238 MS.gene055388:intron 35.0%
!! ATGTCTTTGAGGTCTATGAT+GGG + chr8.1:86798643-86798662 None:intergenic 35.0%
!! GAAAGTACTATCAGTATGCT+TGG + chr8.1:86799356-86799375 None:intergenic 35.0%
!! GATTCAAAAGTTGGTGTCAA+AGG - chr8.1:86798520-86798539 MS.gene055388:CDS 35.0%
!! TATGTCTTTGAGGTCTATGA+TGG + chr8.1:86798644-86798663 None:intergenic 35.0%
AAAGAATTTGGTTGTGCCGA+AGG - chr8.1:86799268-86799287 MS.gene055388:intron 40.0%
ATAGGAGATCTTCTACAGGT+AGG - chr8.1:86799475-86799494 MS.gene055388:intron 40.0%
ATGTACCTTCAATTGGGTCA+CGG + chr8.1:86800198-86800217 None:intergenic 40.0%
ATTCAGAAAGAAGCTCGCTA+CGG + chr8.1:86800173-86800192 None:intergenic 40.0%
CATGGCATGCACTTTGAATT+TGG + chr8.1:86798702-86798721 None:intergenic 40.0%
CATGTTCCATACTGGAAAGA+TGG - chr8.1:86798576-86798595 MS.gene055388:CDS 40.0%
CCCTGAACCTGAATTAACTA+TGG - chr8.1:86799324-86799343 MS.gene055388:intron 40.0%
CGGCACAACCAAATTCTTTA+AGG + chr8.1:86799267-86799286 None:intergenic 40.0%
CTATGTGTTGTGCTAAGACT+CGG + chr8.1:86800138-86800157 None:intergenic 40.0%
GCCGAAGGACTATTTACTTT+GGG - chr8.1:86799283-86799302 MS.gene055388:intron 40.0%
GTTCATTACCTGCATATTGG+TGG + chr8.1:86798967-86798986 None:intergenic 40.0%
TAAATGGGTCAATGTTCCTC+CGG - chr8.1:86799429-86799448 MS.gene055388:intron 40.0%
TCTGAAAAAATCCCCACTCA+TGG + chr8.1:86798720-86798739 None:intergenic 40.0%
TCTGAAAGCAGCTCCTTAAT+AGG + chr8.1:86800235-86800254 None:intergenic 40.0%
TGCCGAAGGACTATTTACTT+TGG - chr8.1:86799282-86799301 MS.gene055388:intron 40.0%
! CCCAATTGAAGGTACATCAA+AGG - chr8.1:86800201-86800220 MS.gene055388:CDS 40.0%
! CCCATAGTTAATTCAGGTTC+AGG + chr8.1:86799328-86799347 None:intergenic 40.0%
! GGAGCTCTTGTTGTAAACAT+AGG - chr8.1:86799457-86799476 MS.gene055388:intron 40.0%
! TCATCTGGTTTGAATGGATG+AGG + chr8.1:86798940-86798959 None:intergenic 40.0%
! TTGGCGCATTTTTATGCAGA+AGG - chr8.1:86800298-86800317 MS.gene055388:CDS 40.0%
AAGCTCGCTACGGAAATTCT+CGG + chr8.1:86800163-86800182 None:intergenic 45.0%
AATTCCCGTGACCCAATTGA+AGG - chr8.1:86800190-86800209 MS.gene055388:CDS 45.0%
ACAACAAGAGCTCCATGTAC+CGG + chr8.1:86799451-86799470 None:intergenic 45.0%
ATTCCACGCATGTTCCATAC+TGG - chr8.1:86798568-86798587 MS.gene055388:CDS 45.0%
CGAGTCTTAGCACAACACAT+AGG - chr8.1:86800136-86800155 MS.gene055388:CDS 45.0%
CTTCTACAAGACCAACTTGG+TGG - chr8.1:86799385-86799404 MS.gene055388:intron 45.0%
GGTAGACCTAAACAAGCGAT+TGG + chr8.1:86799505-86799524 None:intergenic 45.0%
GTCGAGCCCATAGTTAATTC+AGG + chr8.1:86799334-86799353 None:intergenic 45.0%
TCAAAGTGCATGCCATGAGT+GGG - chr8.1:86798705-86798724 MS.gene055388:intron 45.0%
TGTACCTTCAATTGGGTCAC+GGG + chr8.1:86800197-86800216 None:intergenic 45.0%
TTCAAAGTGCATGCCATGAG+TGG - chr8.1:86798704-86798723 MS.gene055388:intron 45.0%
TTTCCAGTATGGAACATGCG+TGG + chr8.1:86798574-86798593 None:intergenic 45.0%
! ATGAAGTGCCACCAATATGC+AGG - chr8.1:86798956-86798975 MS.gene055388:intron 45.0%
! CATATTGGTGGCACTTCATC+TGG + chr8.1:86798955-86798974 None:intergenic 45.0%
! GGACTCACTAGCTGAGTTTT+CGG + chr8.1:86798608-86798627 None:intergenic 45.0%
! GGCACTTCATCTGGTTTGAA+TGG + chr8.1:86798946-86798965 None:intergenic 45.0%
! GTTAATTCAGGTTCAGGGCA+TGG + chr8.1:86799322-86799341 None:intergenic 45.0%
!! AGTTGGTGTCAAAGGTTTGG+TGG - chr8.1:86798528-86798547 MS.gene055388:CDS 45.0%
!! TCAAAGGTTTGGTGGAGTCT+GGG - chr8.1:86798536-86798555 MS.gene055388:CDS 45.0%
AGCTCGCTACGGAAATTCTC+GGG + chr8.1:86800162-86800181 None:intergenic 50.0%
ATTACCTCTACACGTCGAGC+TGG + chr8.1:86798676-86798695 None:intergenic 50.0%
CAAAGTGCATGCCATGAGTG+GGG - chr8.1:86798706-86798725 MS.gene055388:intron 50.0%
GAACTTGAAGGCCACCAAGT+TGG + chr8.1:86799399-86799418 None:intergenic 50.0%
GGCAGTCCAATCGCTTGTTT+AGG - chr8.1:86799496-86799515 MS.gene055388:intron 50.0%
GTCAATGTTCCTCCGGTACA+TGG - chr8.1:86799436-86799455 MS.gene055388:intron 50.0%
TCACAACTTGAAGGGCTCCA+AGG + chr8.1:86800341-86800360 None:intergenic 50.0%
! AATTCAGGTTCAGGGCATGG+TGG + chr8.1:86799319-86799338 None:intergenic 50.0%
!! CAAAGGTTTGGTGGAGTCTG+GGG - chr8.1:86798537-86798556 MS.gene055388:CDS 50.0%
!! GAGTTGTTGTCAGAGGCACT+AGG - chr8.1:86799226-86799245 MS.gene055388:intron 50.0%
!! GTCAAAGGTTTGGTGGAGTC+TGG - chr8.1:86798535-86798554 MS.gene055388:CDS 50.0%
ACAAGAGCTCCATGTACCGG+AGG + chr8.1:86799448-86799467 None:intergenic 55.0%
CAATCCAGCTCGACGTGTAG+AGG - chr8.1:86798669-86798688 MS.gene055388:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 86798439 86800360 86798439 ID=MS.gene055388
chr8.1 mRNA 86798439 86800360 86798439 ID=MS.gene055388.t1;Parent=MS.gene055388
chr8.1 exon 86800109 86800360 86800109 ID=MS.gene055388.t1.exon1;Parent=MS.gene055388.t1
chr8.1 CDS 86800109 86800360 86800109 ID=cds.MS.gene055388.t1;Parent=MS.gene055388.t1
chr8.1 exon 86798439 86798699 86798439 ID=MS.gene055388.t1.exon2;Parent=MS.gene055388.t1
chr8.1 CDS 86798439 86798699 86798439 ID=cds.MS.gene055388.t1;Parent=MS.gene055388.t1
Gene Sequence

>MS.gene055388

ATGGTGGTGAAAAACACAAACCAATTAGAGGAAACCACTAATTCCACTTATGATAGAGAAGTTGAAGTGAAAGCTTTTGATGATTCAAAAGTTGGTGTCAAAGGTTTGGTGGAGTCTGGGGTGTCGAAGATTCCACGCATGTTCCATACTGGAAAGATGGAAATCTCCGAAAACTCAGCTAGTGAGTCCAAGTTGAGTATTCCCATCATAGACCTCAAAGACATACACAACAATCCAGCTCGACGTGTAGAGGTAATTAGCCAAATTCAAAGTGCATGCCATGAGTGGGGATTTTTTCAGATAATTAATCATGAAATTCCCGTTAATGTCTTGGATGAAATGATTGATGGAATTAGTAAGTTTCACGAACAAGATGCTAATGTAAGAAAAGAGTTTTATACGCGGGATTTGAACAAAAAAGTTATGTATTACTCTAATGTACTCTTGTTTAGTGGAAAACCTGCTAATTGGAGAGATACATTTGGTTTTGCTATTGCTCCTCATCCATTCAAACCAGATGAAGTGCCACCAATATGCAGGTAATGAACTCAATATTAATTTGGATTCTCACTCTCAAAATCATAATCAAATTTAATTGTATATCTAGGCCTTTATTTATGGATTATATATGAATTCTCCTCTCATGGAATCATGATCAAAAGTGCATTTCAGTAACAAAGCATGTACATTTGAATTATTATAGTCATTGATGGATTGATTGCATACTTGCAGAGATATTGTGATTGAATATTCACAGAAAATAAAGAATTTGGGTTTCAAAATCTTTGAGTTGTTGTCAGAGGCACTAGGATTTAACCCTTCTTACCTTAAAGAATTTGGTTGTGCCGAAGGACTATTTACTTTGGGTCACTTCTATCCACCATGCCCTGAACCTGAATTAACTATGGGCTCGACCAAGCATACTGATAGTACTTTCATGACATTACTTCTACAAGACCAACTTGGTGGCCTTCAAGTTCTTCATGAAGATAAATGGGTCAATGTTCCTCCGGTACATGGAGCTCTTGTTGTAAACATAGGAGATCTTCTACAGGTAGGCAGTCCAATCGCTTGTTTAGGTCTACCAACAAAAATTTTAGTATTAAAGAAATTTCTATGATTAGTTAGCCTAGTCTTGAAGAATGAAGTTTATCATTGCTTGAGCGAATACCACATTTATTTTTGTAAAACCCCTAATTTTGAAAAGGCATCACATAAAATTTTACTTTAAGCTTAAAAATATATTATGGCAGCCATAAGATTCAAAACAATATTCAGTAAAAGGTTCTATTATTTTGATTGAACTCTTAATAATTATATAAAAACTGAGTAATGCTTGTAGAAAGTATTCCCTTACTATGCCATAAGATTTACAACTATGCTCAAATATTATATTTTCCATGCTATGACTTTAAGACTTTAGTGATTCAAATAATCTCAGTCAAACACTCTCTTCTTACTTAAGAACCACTTGTATATTGGTCATCTATTTGCTTGTATTTTTATTTGCAGAGAAAATAATGACTGAACCTTAGAATGTGATAATATTATAGTGAACTGCAATTGTTGGCTAGAAACCATTTCTAAAAACAAAATCGTTGATTTCTTCATCATTGAAAGTTTGTGGTAATAGTTTTATCATTTTTTTTATACTCTTGCAGCTTATCACAAATGACAAATTTGTAAGTGTTTTTCACCGAGTCTTAGCACAACACATAGGCCCGAGAATTTCCGTAGCGAGCTTCTTTCTGAATTCCCGTGACCCAATTGAAGGTACATCAAAGGTTTATGGTCCTATTAAGGAGCTGCTTTCAGAAGAAAACCCACCAATCTACAAAGACATTACCATAAAAGATTTCTTGGCGCATTTTTATGCAGAAGGCCTTAATGGAAAATCTTCCTTGGAGCCCTTCAAGTTGTGA
Protein sequence

>MS.gene055388.t1

MVVKNTNQLEETTNSTYDREVEVKAFDDSKVGVKGLVESGVSKIPRMFHTGKMEISENSASESKLSIPIIDLKDIHNNPARRVELITNDKFVSVFHRVLAQHIGPRISVASFFLNSRDPIEGTSKVYGPIKELLSEENPPIYKDITIKDFLAHFYAEGLNGKSSLEPFKL