Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26474.t1 | RHN48771.1 | 97.6 | 85 | 2 | 0 | 1 | 85 | 186 | 270 | 5.50E-39 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26474.t1 | Q9SRN0 | 75.9 | 83 | 18 | 1 | 3 | 83 | 34 | 116 | 1.0e-30 | 133.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26474.t1 | A0A072U4E1 | 97.6 | 85 | 2 | 0 | 1 | 85 | 34 | 118 | 4.0e-39 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04921 | MS.gene26474 | 0.804643 | 1.96E-49 | -1.69E-46 |
| MS.gene049281 | MS.gene26474 | 0.817735 | 2.83E-52 | -1.69E-46 |
| MS.gene050082 | MS.gene26474 | 0.812771 | 3.58E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene26474 | -0.83655 | 8.76E-57 | -1.69E-46 |
| MS.gene050338 | MS.gene26474 | 0.820266 | 7.51E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene26474 | -0.815394 | 9.46E-52 | -1.69E-46 |
| MS.gene050521 | MS.gene26474 | 0.802681 | 5.00E-49 | -1.69E-46 |
| MS.gene051044 | MS.gene26474 | 0.842823 | 2.04E-58 | -1.69E-46 |
| MS.gene051138 | MS.gene26474 | 0.856603 | 2.88E-62 | -1.69E-46 |
| MS.gene051364 | MS.gene26474 | 0.84135 | 5.01E-58 | -1.69E-46 |
| MS.gene051366 | MS.gene26474 | 0.83161 | 1.51E-55 | -1.69E-46 |
| MS.gene051367 | MS.gene26474 | 0.805868 | 1.08E-49 | -1.69E-46 |
| MS.gene051422 | MS.gene26474 | 0.840412 | 8.83E-58 | -1.69E-46 |
| MS.gene051440 | MS.gene26474 | 0.823787 | 1.15E-53 | -1.69E-46 |
| MS.gene051517 | MS.gene26474 | 0.816917 | 4.32E-52 | -1.69E-46 |
| MS.gene051651 | MS.gene26474 | 0.846821 | 1.71E-59 | -1.69E-46 |
| MS.gene051973 | MS.gene26474 | 0.851305 | 9.68E-61 | -1.69E-46 |
| MS.gene052129 | MS.gene26474 | 0.802715 | 4.92E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene26474 | 0.809136 | 2.20E-50 | -1.69E-46 |
| MS.gene052289 | MS.gene26474 | 0.809377 | 1.95E-50 | -1.69E-46 |
| MS.gene052494 | MS.gene26474 | 0.841819 | 3.77E-58 | -1.69E-46 |
| MS.gene052606 | MS.gene26474 | 0.848227 | 7.01E-60 | -1.69E-46 |
| MS.gene053097 | MS.gene26474 | 0.856639 | 2.81E-62 | -1.69E-46 |
| MS.gene053218 | MS.gene26474 | 0.800008 | 1.76E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene26474 | 0.805355 | 1.39E-49 | -1.69E-46 |
| MS.gene053887 | MS.gene26474 | 0.830535 | 2.78E-55 | -1.69E-46 |
| MS.gene054343 | MS.gene26474 | -0.823916 | 1.07E-53 | -1.69E-46 |
| MS.gene054344 | MS.gene26474 | -0.81033 | 1.22E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene26474 | -0.843738 | 1.16E-58 | -1.69E-46 |
| MS.gene054884 | MS.gene26474 | 0.803852 | 2.86E-49 | -1.69E-46 |
| MS.gene054959 | MS.gene26474 | 0.80986 | 1.54E-50 | -1.69E-46 |
| MS.gene055158 | MS.gene26474 | 0.803835 | 2.88E-49 | -1.69E-46 |
| MS.gene055251 | MS.gene26474 | 0.822264 | 2.60E-53 | -1.69E-46 |
| MS.gene055388 | MS.gene26474 | 0.812214 | 4.75E-51 | -1.69E-46 |
| MS.gene055719 | MS.gene26474 | 0.810372 | 1.19E-50 | -1.69E-46 |
| MS.gene055729 | MS.gene26474 | 0.802737 | 4.87E-49 | -1.69E-46 |
| MS.gene055731 | MS.gene26474 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene26474 | -0.810285 | 1.24E-50 | -1.69E-46 |
| MS.gene055751 | MS.gene26474 | -0.812262 | 4.63E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene26474 | -0.855493 | 6.08E-62 | -1.69E-46 |
| MS.gene055942 | MS.gene26474 | 0.826457 | 2.68E-54 | -1.69E-46 |
| MS.gene055977 | MS.gene26474 | 0.807036 | 6.15E-50 | -1.69E-46 |
| MS.gene056198 | MS.gene26474 | 0.815388 | 9.48E-52 | -1.69E-46 |
| MS.gene056224 | MS.gene26474 | 0.824565 | 7.53E-54 | -1.69E-46 |
| MS.gene056266 | MS.gene26474 | -0.812005 | 5.27E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene26474 | -0.816398 | 5.65E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene26474 | 0.806346 | 8.60E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene26474 | 0.804838 | 1.78E-49 | -1.69E-46 |
| MS.gene056694 | MS.gene26474 | 0.827275 | 1.71E-54 | -1.69E-46 |
| MS.gene056697 | MS.gene26474 | 0.810069 | 1.38E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene26474 | 0.858804 | 6.40E-63 | -1.69E-46 |
| MS.gene056856 | MS.gene26474 | 0.862643 | 4.38E-64 | -1.69E-46 |
| MS.gene056988 | MS.gene26474 | 0.860182 | 2.47E-63 | -1.69E-46 |
| MS.gene057261 | MS.gene26474 | 0.805692 | 1.18E-49 | -1.69E-46 |
| MS.gene057548 | MS.gene26474 | 0.827769 | 1.30E-54 | -1.69E-46 |
| MS.gene057549 | MS.gene26474 | 0.803491 | 3.40E-49 | -1.69E-46 |
| MS.gene057606 | MS.gene26474 | 0.809602 | 1.74E-50 | -1.69E-46 |
| MS.gene057607 | MS.gene26474 | 0.828515 | 8.61E-55 | -1.69E-46 |
| MS.gene057717 | MS.gene26474 | 0.805095 | 1.57E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene26474 | 0.850711 | 1.42E-60 | -1.69E-46 |
| MS.gene058015 | MS.gene26474 | 0.805561 | 1.26E-49 | -1.69E-46 |
| MS.gene058068 | MS.gene26474 | 0.833708 | 4.57E-56 | -1.69E-46 |
| MS.gene058099 | MS.gene26474 | 0.817733 | 2.83E-52 | -1.69E-46 |
| MS.gene058111 | MS.gene26474 | 0.81341 | 2.60E-51 | -1.69E-46 |
| MS.gene058334 | MS.gene26474 | 0.848274 | 6.80E-60 | -1.69E-46 |
| MS.gene058382 | MS.gene26474 | 0.836226 | 1.06E-56 | -1.69E-46 |
| MS.gene058452 | MS.gene26474 | 0.81668 | 4.88E-52 | -1.69E-46 |
| MS.gene058603 | MS.gene26474 | 0.852625 | 4.08E-61 | -1.69E-46 |
| MS.gene058838 | MS.gene26474 | -0.814362 | 1.60E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene26474 | -0.813046 | 3.12E-51 | -1.69E-46 |
| MS.gene05926 | MS.gene26474 | 0.810395 | 1.18E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene26474 | 0.855045 | 8.21E-62 | -1.69E-46 |
| MS.gene060076 | MS.gene26474 | 0.813169 | 2.93E-51 | -1.69E-46 |
| MS.gene060077 | MS.gene26474 | 0.826905 | 2.10E-54 | -1.69E-46 |
| MS.gene06027 | MS.gene26474 | -0.824455 | 8.00E-54 | -1.69E-46 |
| MS.gene06033 | MS.gene26474 | 0.807751 | 4.34E-50 | -1.69E-46 |
| MS.gene060405 | MS.gene26474 | 0.834408 | 3.05E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene26474 | -0.803134 | 4.03E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene26474 | -0.805312 | 1.42E-49 | -1.69E-46 |
| MS.gene060823 | MS.gene26474 | 0.803602 | 3.22E-49 | -1.69E-46 |
| MS.gene06084 | MS.gene26474 | 0.813716 | 2.22E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene26474 | -0.800619 | 1.32E-48 | -1.69E-46 |
| MS.gene061044 | MS.gene26474 | 0.904982 | 6.94E-80 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTTGAGGTTAAGAAATT+TGG | 0.163407 | 7.3:-8140480 | None:intergenic |
| TTAAGACAGCCCAGATGATT+AGG | 0.339876 | 7.3:-8140395 | None:intergenic |
| AACTGTGACTTGAAAATTTG+AGG | 0.352197 | 7.3:-8140493 | None:intergenic |
| TAGTAACAATCCTAATCATC+TGG | 0.353742 | 7.3:+8140385 | None:intergenic |
| AGAATGAAAGAAGGTTTAGA+AGG | 0.355382 | 7.3:-8140420 | None:intergenic |
| CGGTTCTGTAAGTAATTTGT+TGG | 0.396969 | 7.3:-8140587 | None:intergenic |
| CGTAATCCCAACGACAAAAT+CGG | 0.398168 | 7.3:+8140522 | MS.gene26474:CDS |
| TTGACTCTTACGTCACTTAC+CGG | 0.444690 | 7.3:+8140562 | MS.gene26474:CDS |
| GCCATACCTCCGTCGTATCA+AGG | 0.463364 | 7.3:+8140609 | MS.gene26474:CDS |
| TGTAAGTAATTTGTTGGCTC+CGG | 0.474804 | 7.3:-8140581 | None:intergenic |
| AAGCGTAAACAGTGACATCT+TGG | 0.483243 | 7.3:-8140443 | None:intergenic |
| ACCTTGATACGACGGAGGTA+TGG | 0.495528 | 7.3:-8140610 | None:intergenic |
| GGTCACAAGAGGTTGATGTT+TGG | 0.505405 | 7.3:+8140630 | MS.gene26474:CDS |
| ACATCTTGGAGAATGAAAGA+AGG | 0.562774 | 7.3:-8140429 | None:intergenic |
| AGTAACAATCCTAATCATCT+GGG | 0.581785 | 7.3:+8140386 | None:intergenic |
| CGTCGTATCAAGGTCACAAG+AGG | 0.620521 | 7.3:+8140619 | MS.gene26474:CDS |
| CTCTTGTGACCTTGATACGA+CGG | 0.620962 | 7.3:-8140618 | None:intergenic |
| GGTTAAGAAATTTGGAACAG+TGG | 0.622409 | 7.3:-8140472 | None:intergenic |
| TTGATACGACGGAGGTATGG+CGG | 0.638929 | 7.3:-8140607 | None:intergenic |
| TTGTGACCTTGATACGACGG+AGG | 0.731451 | 7.3:-8140615 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTGAGGTTAAGAAATT+TGG | - | chr7.3:8140483-8140502 | None:intergenic | 20.0% |
| AACTGTGACTTGAAAATTTG+AGG | - | chr7.3:8140496-8140515 | None:intergenic | 30.0% | |
| AGAATGAAAGAAGGTTTAGA+AGG | - | chr7.3:8140423-8140442 | None:intergenic | 30.0% | |
| ACATCTTGGAGAATGAAAGA+AGG | - | chr7.3:8140432-8140451 | None:intergenic | 35.0% | |
| ! | CGGTTCTGTAAGTAATTTGT+TGG | - | chr7.3:8140590-8140609 | None:intergenic | 35.0% |
| ! | GGTTAAGAAATTTGGAACAG+TGG | - | chr7.3:8140475-8140494 | None:intergenic | 35.0% |
| !! | TGTAAGTAATTTGTTGGCTC+CGG | - | chr7.3:8140584-8140603 | None:intergenic | 35.0% |
| !!! | TAGATACCGATTTTGTCGTT+GGG | - | chr7.3:8140531-8140550 | None:intergenic | 35.0% |
| AAGCGTAAACAGTGACATCT+TGG | - | chr7.3:8140446-8140465 | None:intergenic | 40.0% | |
| CCCAGATGATTAGGATTGTT+AGG | - | chr7.3:8140389-8140408 | None:intergenic | 40.0% | |
| CGTAATCCCAACGACAAAAT+CGG | + | chr7.3:8140522-8140541 | MS.gene26474:CDS | 40.0% | |
| TTAAGACAGCCCAGATGATT+AGG | - | chr7.3:8140398-8140417 | None:intergenic | 40.0% | |
| TTGACTCTTACGTCACTTAC+CGG | + | chr7.3:8140562-8140581 | MS.gene26474:CDS | 40.0% | |
| !!! | GTAGATACCGATTTTGTCGT+TGG | - | chr7.3:8140532-8140551 | None:intergenic | 40.0% |
| CTCTTGTGACCTTGATACGA+CGG | - | chr7.3:8140621-8140640 | None:intergenic | 45.0% | |
| !! | GGTCACAAGAGGTTGATGTT+TGG | + | chr7.3:8140630-8140649 | MS.gene26474:CDS | 45.0% |
| ACCTTGATACGACGGAGGTA+TGG | - | chr7.3:8140613-8140632 | None:intergenic | 50.0% | |
| CGTCGTATCAAGGTCACAAG+AGG | + | chr7.3:8140619-8140638 | MS.gene26474:CDS | 50.0% | |
| TTGTGACCTTGATACGACGG+AGG | - | chr7.3:8140618-8140637 | None:intergenic | 50.0% | |
| ! | TTGATACGACGGAGGTATGG+CGG | - | chr7.3:8140610-8140629 | None:intergenic | 50.0% |
| GCCATACCTCCGTCGTATCA+AGG | + | chr7.3:8140609-8140628 | MS.gene26474:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 8140388 | 8140657 | 8140388 | ID=MS.gene26474 |
| chr7.3 | mRNA | 8140388 | 8140657 | 8140388 | ID=MS.gene26474.t1;Parent=MS.gene26474 |
| chr7.3 | exon | 8140388 | 8140657 | 8140388 | ID=MS.gene26474.t1.exon1;Parent=MS.gene26474.t1 |
| chr7.3 | CDS | 8140388 | 8140657 | 8140388 | ID=cds.MS.gene26474.t1;Parent=MS.gene26474.t1 |
| Gene Sequence |
| Protein sequence |
