Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26475.t1 | XP_013450198.1 | 99.5 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 9.10E-117 | 429.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26475.t1 | Q9SRN0 | 62.9 | 210 | 75 | 2 | 3 | 210 | 1 | 209 | 1.5e-75 | 283.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26475.t1 | A0A072U4E1 | 99.5 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 6.5e-117 | 429.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene26475 | -0.819156 | 1.35E-52 | -1.69E-46 |
| MS.gene049281 | MS.gene26475 | 0.801289 | 9.66E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene26475 | 0.806047 | 9.94E-50 | -1.69E-46 |
| MS.gene049549 | MS.gene26475 | 0.816179 | 6.32E-52 | -1.69E-46 |
| MS.gene050082 | MS.gene26475 | 0.809781 | 1.60E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene26475 | -0.847481 | 1.13E-59 | -1.69E-46 |
| MS.gene050338 | MS.gene26475 | 0.851805 | 6.98E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene26475 | -0.810759 | 9.82E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene26475 | -0.80059 | 1.34E-48 | -1.69E-46 |
| MS.gene050809 | MS.gene26475 | 0.81055 | 1.09E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene26475 | -0.810244 | 1.27E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene26475 | -0.807812 | 4.21E-50 | -1.69E-46 |
| MS.gene051044 | MS.gene26475 | 0.837309 | 5.61E-57 | -1.69E-46 |
| MS.gene05104 | MS.gene26475 | 0.805459 | 1.32E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene26475 | 0.852254 | 5.21E-61 | -1.69E-46 |
| MS.gene051364 | MS.gene26475 | 0.823513 | 1.33E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene26475 | 0.811131 | 8.16E-51 | -1.69E-46 |
| MS.gene051422 | MS.gene26475 | 0.839263 | 1.76E-57 | -1.69E-46 |
| MS.gene051439 | MS.gene26475 | 0.825973 | 3.50E-54 | -1.69E-46 |
| MS.gene051440 | MS.gene26475 | 0.824807 | 6.61E-54 | -1.69E-46 |
| MS.gene051517 | MS.gene26475 | 0.821411 | 4.10E-53 | -1.69E-46 |
| MS.gene051552 | MS.gene26475 | 0.8039 | 2.80E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene26475 | 0.846798 | 1.73E-59 | -1.69E-46 |
| MS.gene051749 | MS.gene26475 | 0.80874 | 2.67E-50 | -1.69E-46 |
| MS.gene051973 | MS.gene26475 | 0.856888 | 2.37E-62 | -1.69E-46 |
| MS.gene052129 | MS.gene26475 | 0.819839 | 9.41E-53 | -1.69E-46 |
| MS.gene052211 | MS.gene26475 | 0.81997 | 8.78E-53 | -1.69E-46 |
| MS.gene052289 | MS.gene26475 | 0.832078 | 1.16E-55 | -1.69E-46 |
| MS.gene05240 | MS.gene26475 | 0.814064 | 1.86E-51 | -1.69E-46 |
| MS.gene052494 | MS.gene26475 | 0.844881 | 5.74E-59 | -1.69E-46 |
| MS.gene052606 | MS.gene26475 | 0.862981 | 3.45E-64 | -1.69E-46 |
| MS.gene052823 | MS.gene26475 | -0.80502 | 1.63E-49 | -1.69E-46 |
| MS.gene052825 | MS.gene26475 | -0.800271 | 1.56E-48 | -1.69E-46 |
| MS.gene053097 | MS.gene26475 | 0.855941 | 4.50E-62 | -1.69E-46 |
| MS.gene053203 | MS.gene26475 | -0.805739 | 1.15E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene26475 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene26475 | -0.815856 | 7.46E-52 | -1.69E-46 |
| MS.gene053887 | MS.gene26475 | 0.834767 | 2.48E-56 | -1.69E-46 |
| MS.gene054343 | MS.gene26475 | -0.833832 | 4.25E-56 | -1.69E-46 |
| MS.gene054344 | MS.gene26475 | -0.823193 | 1.58E-53 | -1.69E-46 |
| MS.gene054706 | MS.gene26475 | 0.808545 | 2.94E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene26475 | -0.851981 | 6.23E-61 | -1.69E-46 |
| MS.gene054884 | MS.gene26475 | 0.832574 | 8.75E-56 | -1.69E-46 |
| MS.gene054959 | MS.gene26475 | 0.800658 | 1.30E-48 | -1.69E-46 |
| MS.gene055158 | MS.gene26475 | 0.81899 | 1.47E-52 | -1.69E-46 |
| MS.gene055240 | MS.gene26475 | 0.804288 | 2.32E-49 | -1.69E-46 |
| MS.gene055251 | MS.gene26475 | 0.824825 | 6.54E-54 | -1.69E-46 |
| MS.gene055388 | MS.gene26475 | 0.821245 | 4.47E-53 | -1.69E-46 |
| MS.gene055729 | MS.gene26475 | 0.829801 | 4.20E-55 | -1.69E-46 |
| MS.gene055730 | MS.gene26475 | 0.824654 | 7.18E-54 | -1.69E-46 |
| MS.gene055731 | MS.gene26475 | 0.839295 | 1.73E-57 | -1.69E-46 |
| MS.gene055748 | MS.gene26475 | -0.826415 | 2.75E-54 | -1.69E-46 |
| MS.gene055751 | MS.gene26475 | -0.821209 | 4.56E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene26475 | -0.871573 | 6.16E-67 | -1.69E-46 |
| MS.gene055942 | MS.gene26475 | 0.80036 | 1.50E-48 | -1.69E-46 |
| MS.gene055977 | MS.gene26475 | 0.821658 | 3.59E-53 | -1.69E-46 |
| MS.gene056198 | MS.gene26475 | 0.823787 | 1.15E-53 | -1.69E-46 |
| MS.gene056218 | MS.gene26475 | 0.804863 | 1.76E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene26475 | 0.83198 | 1.23E-55 | -1.69E-46 |
| MS.gene056266 | MS.gene26475 | -0.817772 | 2.77E-52 | -1.69E-46 |
| MS.gene056387 | MS.gene26475 | -0.813464 | 2.53E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene26475 | -0.825822 | 3.80E-54 | -1.69E-46 |
| MS.gene056590 | MS.gene26475 | 0.838481 | 2.80E-57 | -1.69E-46 |
| MS.gene056591 | MS.gene26475 | 0.836802 | 7.56E-57 | -1.69E-46 |
| MS.gene056692 | MS.gene26475 | -0.801207 | 1.00E-48 | -1.69E-46 |
| MS.gene056694 | MS.gene26475 | 0.844632 | 6.70E-59 | -1.69E-46 |
| MS.gene056697 | MS.gene26475 | 0.843205 | 1.62E-58 | -1.69E-46 |
| MS.gene056713 | MS.gene26475 | 0.800716 | 1.27E-48 | -1.69E-46 |
| MS.gene056748 | MS.gene26475 | 0.857635 | 1.43E-62 | -1.69E-46 |
| MS.gene056856 | MS.gene26475 | 0.870811 | 1.10E-66 | -1.69E-46 |
| MS.gene056988 | MS.gene26475 | 0.8694 | 3.19E-66 | -1.69E-46 |
| MS.gene057261 | MS.gene26475 | 0.829259 | 5.69E-55 | -1.69E-46 |
| MS.gene057548 | MS.gene26475 | 0.823336 | 1.46E-53 | -1.69E-46 |
| MS.gene057549 | MS.gene26475 | 0.80082 | 1.20E-48 | -1.69E-46 |
| MS.gene057607 | MS.gene26475 | 0.819798 | 9.61E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene26475 | -0.812699 | 3.72E-51 | -1.69E-46 |
| MS.gene057717 | MS.gene26475 | 0.838469 | 2.82E-57 | -1.69E-46 |
| MS.gene057768 | MS.gene26475 | 0.850355 | 1.79E-60 | -1.69E-46 |
| MS.gene057820 | MS.gene26475 | 0.803434 | 3.49E-49 | -1.69E-46 |
| MS.gene058068 | MS.gene26475 | 0.833765 | 4.42E-56 | -1.69E-46 |
| MS.gene058099 | MS.gene26475 | 0.828115 | 1.08E-54 | -1.69E-46 |
| MS.gene058111 | MS.gene26475 | 0.811383 | 7.20E-51 | -1.69E-46 |
| MS.gene058175 | MS.gene26475 | 0.802965 | 4.37E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene26475 | 0.848263 | 6.85E-60 | -1.69E-46 |
| MS.gene05837 | MS.gene26475 | -0.811849 | 5.70E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene26475 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene058382 | MS.gene26475 | 0.834904 | 2.29E-56 | -1.69E-46 |
| MS.gene058418 | MS.gene26475 | 0.824017 | 1.01E-53 | -1.69E-46 |
| MS.gene058452 | MS.gene26475 | 0.82813 | 1.07E-54 | -1.69E-46 |
| MS.gene058544 | MS.gene26475 | -0.803013 | 4.27E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene26475 | 0.856314 | 3.50E-62 | -1.69E-46 |
| MS.gene058838 | MS.gene26475 | -0.846256 | 2.44E-59 | -1.69E-46 |
| MS.gene059023 | MS.gene26475 | -0.81669 | 4.86E-52 | -1.69E-46 |
| MS.gene05914 | MS.gene26475 | -0.825394 | 4.80E-54 | -1.69E-46 |
| MS.gene05926 | MS.gene26475 | 0.832919 | 7.18E-56 | -1.69E-46 |
| MS.gene059304 | MS.gene26475 | 0.802899 | 4.51E-49 | -1.69E-46 |
| MS.gene059315 | MS.gene26475 | 0.806581 | 7.67E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene26475 | 0.884723 | 1.50E-71 | -1.69E-46 |
| MS.gene059955 | MS.gene26475 | 0.812824 | 3.49E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene26475 | -0.810095 | 1.37E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene26475 | 0.812086 | 5.06E-51 | -1.69E-46 |
| MS.gene06027 | MS.gene26475 | -0.836524 | 8.90E-57 | -1.69E-46 |
| MS.gene060358 | MS.gene26475 | 0.804709 | 1.90E-49 | -1.69E-46 |
| MS.gene060405 | MS.gene26475 | 0.850391 | 1.75E-60 | -1.69E-46 |
| MS.gene060762 | MS.gene26475 | -0.816061 | 6.71E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene26475 | -0.811001 | 8.71E-51 | -1.69E-46 |
| MS.gene060823 | MS.gene26475 | 0.8068 | 6.90E-50 | -1.69E-46 |
| MS.gene06084 | MS.gene26475 | 0.836234 | 1.05E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene26475 | -0.80492 | 1.71E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene26475 | 0.899037 | 2.95E-77 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26475.t1 | MTR_7g106050 | 99.524 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 2.84e-155 | 428 |
| MS.gene26475.t1 | MTR_1g057420 | 71.429 | 210 | 59 | 1 | 1 | 210 | 1 | 209 | 8.76e-108 | 308 |
| MS.gene26475.t1 | MTR_2g086790 | 49.772 | 219 | 98 | 2 | 4 | 210 | 3 | 221 | 7.06e-73 | 220 |
| MS.gene26475.t1 | MTR_7g106080 | 99.048 | 105 | 1 | 0 | 106 | 210 | 7 | 111 | 7.16e-73 | 216 |
| MS.gene26475.t1 | MTR_4g051880 | 49.545 | 220 | 100 | 4 | 1 | 210 | 1 | 219 | 1.02e-67 | 207 |
| MS.gene26475.t1 | MTR_3g092790 | 39.891 | 183 | 109 | 1 | 2 | 184 | 8 | 189 | 3.58e-38 | 131 |
| MS.gene26475.t1 | MTR_7g118270 | 34.574 | 188 | 114 | 3 | 24 | 210 | 50 | 229 | 1.10e-34 | 123 |
| MS.gene26475.t1 | MTR_8g024250 | 37.013 | 154 | 97 | 0 | 24 | 177 | 102 | 255 | 5.60e-24 | 96.3 |
| MS.gene26475.t1 | MTR_2g011350 | 31.613 | 155 | 100 | 4 | 33 | 184 | 37 | 188 | 1.22e-20 | 85.9 |
| MS.gene26475.t1 | MTR_5g087760 | 30.726 | 179 | 115 | 4 | 12 | 181 | 56 | 234 | 7.21e-18 | 79.7 |
| MS.gene26475.t1 | MTR_6g071410 | 30.380 | 158 | 95 | 4 | 24 | 172 | 15 | 166 | 2.19e-15 | 72.4 |
| MS.gene26475.t1 | MTR_6g070890 | 30.380 | 158 | 95 | 4 | 24 | 172 | 15 | 166 | 2.19e-15 | 72.4 |
| MS.gene26475.t1 | MTR_6g070900 | 30.323 | 155 | 94 | 3 | 20 | 166 | 11 | 159 | 4.38e-15 | 71.2 |
| MS.gene26475.t1 | MTR_6g071400 | 30.323 | 155 | 94 | 3 | 20 | 166 | 11 | 159 | 4.38e-15 | 71.2 |
| MS.gene26475.t1 | MTR_6g471090 | 28.289 | 152 | 102 | 4 | 19 | 166 | 9 | 157 | 1.77e-14 | 69.7 |
| MS.gene26475.t1 | MTR_3g063310 | 28.834 | 163 | 114 | 2 | 34 | 195 | 83 | 244 | 6.94e-14 | 68.6 |
| MS.gene26475.t1 | MTR_1g105570 | 25.153 | 163 | 117 | 2 | 27 | 189 | 58 | 215 | 4.01e-13 | 66.2 |
| MS.gene26475.t1 | MTR_1g057270 | 25.625 | 160 | 117 | 2 | 36 | 194 | 73 | 231 | 1.39e-12 | 65.1 |
| MS.gene26475.t1 | MTR_7g106010 | 25.806 | 186 | 131 | 5 | 8 | 187 | 25 | 209 | 2.90e-11 | 61.2 |
| MS.gene26475.t1 | MTR_7g105870 | 27.607 | 163 | 115 | 3 | 17 | 177 | 40 | 201 | 4.19e-11 | 60.5 |
| MS.gene26475.t1 | MTR_7g106000 | 26.380 | 163 | 114 | 4 | 20 | 177 | 41 | 202 | 6.61e-11 | 60.1 |
| MS.gene26475.t1 | MTR_6g471080 | 28.931 | 159 | 105 | 5 | 19 | 172 | 6 | 161 | 6.75e-11 | 59.7 |
| MS.gene26475.t1 | MTR_1g057400 | 24.064 | 187 | 129 | 5 | 10 | 183 | 3 | 189 | 8.45e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26475.t1 | AT3G11660 | 64.762 | 210 | 71 | 2 | 3 | 210 | 1 | 209 | 1.55e-102 | 295 |
| MS.gene26475.t1 | AT3G52470 | 61.353 | 207 | 79 | 1 | 4 | 210 | 3 | 208 | 2.26e-94 | 274 |
| MS.gene26475.t1 | AT3G44220 | 56.190 | 210 | 88 | 1 | 1 | 210 | 1 | 206 | 1.33e-84 | 249 |
| MS.gene26475.t1 | AT2G35960 | 57.346 | 211 | 88 | 2 | 1 | 210 | 1 | 210 | 3.02e-82 | 243 |
| MS.gene26475.t1 | AT5G06330 | 55.189 | 212 | 88 | 2 | 1 | 210 | 1 | 207 | 5.04e-76 | 228 |
| MS.gene26475.t1 | AT4G09590 | 49.763 | 211 | 103 | 2 | 1 | 209 | 1 | 210 | 1.10e-74 | 224 |
| MS.gene26475.t1 | AT2G35970 | 50.237 | 211 | 102 | 2 | 1 | 209 | 1 | 210 | 1.98e-74 | 224 |
| MS.gene26475.t1 | AT5G22200 | 52.336 | 214 | 94 | 2 | 1 | 210 | 1 | 210 | 9.67e-72 | 217 |
| MS.gene26475.t1 | AT5G53730 | 37.634 | 186 | 113 | 2 | 2 | 184 | 8 | 193 | 1.31e-44 | 148 |
| MS.gene26475.t1 | AT4G01410 | 38.372 | 172 | 106 | 0 | 18 | 189 | 42 | 213 | 2.95e-36 | 127 |
| MS.gene26475.t1 | AT5G05657 | 46.875 | 128 | 56 | 5 | 40 | 161 | 11 | 132 | 1.23e-26 | 99.8 |
| MS.gene26475.t1 | AT5G22870 | 30.380 | 158 | 109 | 1 | 20 | 177 | 29 | 185 | 5.14e-21 | 87.0 |
| MS.gene26475.t1 | AT2G35980 | 35.135 | 148 | 92 | 4 | 20 | 164 | 40 | 186 | 6.64e-21 | 87.0 |
| MS.gene26475.t1 | AT1G65690 | 25.275 | 182 | 135 | 1 | 21 | 202 | 70 | 250 | 7.78e-16 | 73.9 |
| MS.gene26475.t1 | AT4G05220 | 23.529 | 153 | 116 | 1 | 25 | 177 | 51 | 202 | 1.95e-12 | 64.3 |
| MS.gene26475.t1 | AT1G61760 | 22.699 | 163 | 124 | 2 | 16 | 177 | 39 | 200 | 2.10e-12 | 64.3 |
| MS.gene26475.t1 | AT5G06320 | 26.250 | 160 | 116 | 2 | 20 | 177 | 47 | 206 | 2.70e-12 | 63.9 |
Find 50 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTTGAGGTTAAGAAATT+TGG | 0.163407 | 7.3:-8022323 | None:intergenic |
| GTTTGGTCGCCGTTTGTTTA+CGG | 0.302323 | 7.3:+8022491 | MS.gene26475:CDS |
| TTAAGACAGCCCAGATGATT+AGG | 0.339876 | 7.3:-8022238 | None:intergenic |
| AACTGTGACTTGAAAATTTG+AGG | 0.352197 | 7.3:-8022336 | None:intergenic |
| TAGTAACAATCCTAATCATC+TGG | 0.353742 | 7.3:+8022228 | MS.gene26475:CDS |
| AGAATGAAAGAAGGTTTAGA+AGG | 0.355382 | 7.3:-8022263 | None:intergenic |
| CGGTTCTGTAAGTAATTTGT+TGG | 0.396969 | 7.3:-8022430 | None:intergenic |
| CGTAATCCCAACGACAAAAT+CGG | 0.398168 | 7.3:+8022365 | MS.gene26475:CDS |
| CTAAGAGCAACAGAGTTATA+TGG | 0.400589 | 7.3:-8022533 | None:intergenic |
| TGTCCTGCTTTCATCACGTT+TGG | 0.401371 | 7.3:+8022662 | MS.gene26475:CDS |
| TTAAATTTGACGGTAGGGTT+AGG | 0.405172 | 7.3:+8022591 | MS.gene26475:CDS |
| CGTTTGGTCCAAGGAGTAAC+GGG | 0.423477 | 7.3:+8022678 | MS.gene26475:CDS |
| CTTGTTGTCGTTAAATTTGA+CGG | 0.443892 | 7.3:+8022581 | MS.gene26475:CDS |
| TTGACTCTTACGTCACTTAC+CGG | 0.444690 | 7.3:+8022405 | MS.gene26475:CDS |
| CTTTCATCACGTTTGGTCCA+AGG | 0.447750 | 7.3:+8022669 | MS.gene26475:CDS |
| GTTTGTGTTTCTTTCCTTTG+TGG | 0.449823 | 7.3:-8022157 | None:intergenic |
| TGACGGTAGGGTTAGGTGGA+AGG | 0.453126 | 7.3:+8022598 | MS.gene26475:CDS |
| GCCATACCTCCGTCGTATCA+AGG | 0.463364 | 7.3:+8022452 | MS.gene26475:CDS |
| CGTTACCGTTATCCTGGTCT+TGG | 0.465117 | 7.3:-8022556 | None:intergenic |
| TTGTCGTTAAATTTGACGGT+AGG | 0.466414 | 7.3:+8022585 | MS.gene26475:CDS |
| TGTAAGTAATTTGTTGGCTC+CGG | 0.474804 | 7.3:-8022424 | None:intergenic |
| AAGCGTAAACAGTGACATCT+TGG | 0.483243 | 7.3:-8022286 | None:intergenic |
| GTCACAAAGAGGTTGATGTT+TGG | 0.485771 | 7.3:+8022474 | MS.gene26475:CDS |
| ACGCTAATCCCGTTACTCCT+TGG | 0.495144 | 7.3:-8022686 | None:intergenic |
| ACGTTTGTTCCGTAAACAAA+CGG | 0.495384 | 7.3:-8022500 | None:intergenic |
| ACCTTGATACGACGGAGGTA+TGG | 0.495528 | 7.3:-8022453 | None:intergenic |
| TGTCGTTAAATTTGACGGTA+GGG | 0.515934 | 7.3:+8022586 | MS.gene26475:CDS |
| CAAGAACGTTACCGTTATCC+TGG | 0.525747 | 7.3:-8022562 | None:intergenic |
| AATTTGACGGTAGGGTTAGG+TGG | 0.527468 | 7.3:+8022594 | MS.gene26475:CDS |
| TGTTTACGGAACAAACGTTC+CGG | 0.527883 | 7.3:+8022505 | MS.gene26475:CDS |
| AGATGATTAGGATTGTTACT+AGG | 0.539987 | 7.3:-8022226 | None:intergenic |
| CTTAGCCAAGACCAGGATAA+CGG | 0.544393 | 7.3:+8022551 | MS.gene26475:CDS |
| GGTAGGGTTAGGTGGAAGGT+TGG | 0.557151 | 7.3:+8022602 | MS.gene26475:CDS |
| GGACCAAACGTGATGAAAGC+AGG | 0.559291 | 7.3:-8022665 | None:intergenic |
| ACATCTTGGAGAATGAAAGA+AGG | 0.562774 | 7.3:-8022272 | None:intergenic |
| ACGTTTGGTCCAAGGAGTAA+CGG | 0.568501 | 7.3:+8022677 | MS.gene26475:CDS |
| AGTAACAATCCTAATCATCT+GGG | 0.581785 | 7.3:+8022229 | MS.gene26475:CDS |
| GGGATTAGCGTCGGTGATAG+CGG | 0.588222 | 7.3:+8022698 | MS.gene26475:CDS |
| ACAGAGTTATATGGAGCTAC+CGG | 0.593640 | 7.3:-8022524 | None:intergenic |
| TGTGTTTCTTTCCTTTGTGG+TGG | 0.600067 | 7.3:-8022154 | None:intergenic |
| GGTTAAGAAATTTGGAACAG+TGG | 0.622409 | 7.3:-8022315 | None:intergenic |
| TGTTGCTCTTAGCCAAGACC+AGG | 0.627706 | 7.3:+8022544 | MS.gene26475:CDS |
| TTGATACGACGGAGGTATGG+CGG | 0.638929 | 7.3:-8022450 | None:intergenic |
| TTGTTACTAGGACGATGAAG+AGG | 0.645861 | 7.3:-8022214 | None:intergenic |
| TTAAGTATCAGATTGTTCAA+CGG | 0.648896 | 7.3:+8022726 | MS.gene26475:CDS |
| AGGAGTAACGGGATTAGCGT+CGG | 0.659748 | 7.3:+8022689 | MS.gene26475:CDS |
| TCTTTGTGACCTTGATACGA+CGG | 0.662874 | 7.3:-8022461 | None:intergenic |
| AAAGAGTGCAACCACCACAA+AGG | 0.679737 | 7.3:+8022143 | MS.gene26475:CDS |
| GTCGTATCAAGGTCACAAAG+AGG | 0.691156 | 7.3:+8022463 | MS.gene26475:CDS |
| TTGTGACCTTGATACGACGG+AGG | 0.700889 | 7.3:-8022458 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTGAGGTTAAGAAATT+TGG | - | chr7.3:8022326-8022345 | None:intergenic | 20.0% |
| ! | TTAAGTATCAGATTGTTCAA+CGG | + | chr7.3:8022726-8022745 | MS.gene26475:CDS | 25.0% |
| AACTGTGACTTGAAAATTTG+AGG | - | chr7.3:8022339-8022358 | None:intergenic | 30.0% | |
| AGAATGAAAGAAGGTTTAGA+AGG | - | chr7.3:8022266-8022285 | None:intergenic | 30.0% | |
| AGATGATTAGGATTGTTACT+AGG | - | chr7.3:8022229-8022248 | None:intergenic | 30.0% | |
| AGTAACAATCCTAATCATCT+GGG | + | chr7.3:8022229-8022248 | MS.gene26475:CDS | 30.0% | |
| TAGTAACAATCCTAATCATC+TGG | + | chr7.3:8022228-8022247 | MS.gene26475:CDS | 30.0% | |
| ! | CTTGTTGTCGTTAAATTTGA+CGG | + | chr7.3:8022581-8022600 | MS.gene26475:CDS | 30.0% |
| AACGCTTCAGAAAAATCTTC+TGG | + | chr7.3:8022177-8022196 | MS.gene26475:CDS | 35.0% | |
| ACATCTTGGAGAATGAAAGA+AGG | - | chr7.3:8022275-8022294 | None:intergenic | 35.0% | |
| ACGCTTCAGAAAAATCTTCT+GGG | + | chr7.3:8022178-8022197 | MS.gene26475:CDS | 35.0% | |
| ACGTTTGTTCCGTAAACAAA+CGG | - | chr7.3:8022503-8022522 | None:intergenic | 35.0% | |
| CAGGACAACGAACAAAAATA+TGG | - | chr7.3:8022649-8022668 | None:intergenic | 35.0% | |
| CTAAGAGCAACAGAGTTATA+TGG | - | chr7.3:8022536-8022555 | None:intergenic | 35.0% | |
| GTTTGTGTTTCTTTCCTTTG+TGG | - | chr7.3:8022160-8022179 | None:intergenic | 35.0% | |
| TGTCGTTAAATTTGACGGTA+GGG | + | chr7.3:8022586-8022605 | MS.gene26475:CDS | 35.0% | |
| TTAAATTTGACGGTAGGGTT+AGG | + | chr7.3:8022591-8022610 | MS.gene26475:CDS | 35.0% | |
| TTGTCGTTAAATTTGACGGT+AGG | + | chr7.3:8022585-8022604 | MS.gene26475:CDS | 35.0% | |
| ! | CGGTTCTGTAAGTAATTTGT+TGG | - | chr7.3:8022433-8022452 | None:intergenic | 35.0% |
| ! | GGTTAAGAAATTTGGAACAG+TGG | - | chr7.3:8022318-8022337 | None:intergenic | 35.0% |
| !! | TGTAAGTAATTTGTTGGCTC+CGG | - | chr7.3:8022427-8022446 | None:intergenic | 35.0% |
| !!! | AAGGTTGGTGCTTTTATTTC+TGG | + | chr7.3:8022617-8022636 | MS.gene26475:CDS | 35.0% |
| !!! | TAGATACCGATTTTGTCGTT+GGG | - | chr7.3:8022374-8022393 | None:intergenic | 35.0% |
| AAGCGTAAACAGTGACATCT+TGG | - | chr7.3:8022289-8022308 | None:intergenic | 40.0% | |
| ACAGAGTTATATGGAGCTAC+CGG | - | chr7.3:8022527-8022546 | None:intergenic | 40.0% | |
| CGCTTCAGAAAAATCTTCTG+GGG | + | chr7.3:8022179-8022198 | MS.gene26475:CDS | 40.0% | |
| CGTAATCCCAACGACAAAAT+CGG | + | chr7.3:8022365-8022384 | MS.gene26475:CDS | 40.0% | |
| TCTTTGTGACCTTGATACGA+CGG | - | chr7.3:8022464-8022483 | None:intergenic | 40.0% | |
| TGTGTTTCTTTCCTTTGTGG+TGG | - | chr7.3:8022157-8022176 | None:intergenic | 40.0% | |
| TGTTTACGGAACAAACGTTC+CGG | + | chr7.3:8022505-8022524 | MS.gene26475:CDS | 40.0% | |
| TTAAGACAGCCCAGATGATT+AGG | - | chr7.3:8022241-8022260 | None:intergenic | 40.0% | |
| TTGACTCTTACGTCACTTAC+CGG | + | chr7.3:8022405-8022424 | MS.gene26475:CDS | 40.0% | |
| TTGTTACTAGGACGATGAAG+AGG | - | chr7.3:8022217-8022236 | None:intergenic | 40.0% | |
| !! | GTCACAAAGAGGTTGATGTT+TGG | + | chr7.3:8022474-8022493 | MS.gene26475:CDS | 40.0% |
| !!! | GTAGATACCGATTTTGTCGT+TGG | - | chr7.3:8022375-8022394 | None:intergenic | 40.0% |
| AATTTGACGGTAGGGTTAGG+TGG | + | chr7.3:8022594-8022613 | MS.gene26475:CDS | 45.0% | |
| ACGTTTGGTCCAAGGAGTAA+CGG | + | chr7.3:8022677-8022696 | MS.gene26475:CDS | 45.0% | |
| CAAGAACGTTACCGTTATCC+TGG | - | chr7.3:8022565-8022584 | None:intergenic | 45.0% | |
| CTTAGCCAAGACCAGGATAA+CGG | + | chr7.3:8022551-8022570 | MS.gene26475:CDS | 45.0% | |
| CTTTCATCACGTTTGGTCCA+AGG | + | chr7.3:8022669-8022688 | MS.gene26475:CDS | 45.0% | |
| GTCGTATCAAGGTCACAAAG+AGG | + | chr7.3:8022463-8022482 | MS.gene26475:CDS | 45.0% | |
| GTTTGGTCGCCGTTTGTTTA+CGG | + | chr7.3:8022491-8022510 | MS.gene26475:CDS | 45.0% | |
| TGTCCTGCTTTCATCACGTT+TGG | + | chr7.3:8022662-8022681 | MS.gene26475:CDS | 45.0% | |
| ! | AAAGAGTGCAACCACCACAA+AGG | + | chr7.3:8022143-8022162 | MS.gene26475:CDS | 45.0% |
| ACCTTGATACGACGGAGGTA+TGG | - | chr7.3:8022456-8022475 | None:intergenic | 50.0% | |
| ACGCTAATCCCGTTACTCCT+TGG | - | chr7.3:8022689-8022708 | None:intergenic | 50.0% | |
| CGTTACCGTTATCCTGGTCT+TGG | - | chr7.3:8022559-8022578 | None:intergenic | 50.0% | |
| CGTTTGGTCCAAGGAGTAAC+GGG | + | chr7.3:8022678-8022697 | MS.gene26475:CDS | 50.0% | |
| GGACCAAACGTGATGAAAGC+AGG | - | chr7.3:8022668-8022687 | None:intergenic | 50.0% | |
| TGTTGCTCTTAGCCAAGACC+AGG | + | chr7.3:8022544-8022563 | MS.gene26475:CDS | 50.0% | |
| TTGTGACCTTGATACGACGG+AGG | - | chr7.3:8022461-8022480 | None:intergenic | 50.0% | |
| ! | AGGAGTAACGGGATTAGCGT+CGG | + | chr7.3:8022689-8022708 | MS.gene26475:CDS | 50.0% |
| ! | TTGATACGACGGAGGTATGG+CGG | - | chr7.3:8022453-8022472 | None:intergenic | 50.0% |
| GCCATACCTCCGTCGTATCA+AGG | + | chr7.3:8022452-8022471 | MS.gene26475:CDS | 55.0% | |
| GGTAGGGTTAGGTGGAAGGT+TGG | + | chr7.3:8022602-8022621 | MS.gene26475:CDS | 55.0% | |
| TGACGGTAGGGTTAGGTGGA+AGG | + | chr7.3:8022598-8022617 | MS.gene26475:CDS | 55.0% | |
| !! | GGGATTAGCGTCGGTGATAG+CGG | + | chr7.3:8022698-8022717 | MS.gene26475:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 8022134 | 8022766 | 8022134 | ID=MS.gene26475 |
| chr7.3 | mRNA | 8022134 | 8022766 | 8022134 | ID=MS.gene26475.t1;Parent=MS.gene26475 |
| chr7.3 | exon | 8022134 | 8022766 | 8022134 | ID=MS.gene26475.t1.exon1;Parent=MS.gene26475.t1 |
| chr7.3 | CDS | 8022134 | 8022766 | 8022134 | ID=cds.MS.gene26475.t1;Parent=MS.gene26475.t1 |
| Gene Sequence |
| Protein sequence |
