Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056009.t1 | XP_003591217.2 | 94.7 | 978 | 51 | 1 | 1 | 978 | 1 | 977 | 0.00E+00 | 1792.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056009.t1 | P93655 | 73.6 | 980 | 214 | 8 | 1 | 975 | 1 | 940 | 0.0e+00 | 1380.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056009.t1 | G7I9H7 | 94.7 | 978 | 51 | 1 | 1 | 978 | 1 | 977 | 0.0e+00 | 1792.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056009 | MS.gene065894 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene056009 | MS.gene072054 | 0.820269 | 7.50E-53 | -1.69E-46 |
| MS.gene056009 | MS.gene21022 | 0.955932 | 9.66E-114 | -1.69E-46 |
| MS.gene056009 | MS.gene40791 | 0.984186 | 7.80E-160 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene54210 | MS.gene056009 | PPI |
| MS.gene21502 | MS.gene056009 | PPI |
| MS.gene09688 | MS.gene056009 | PPI |
| MS.gene015346 | MS.gene056009 | PPI |
| MS.gene61239 | MS.gene056009 | PPI |
| MS.gene011621 | MS.gene056009 | PPI |
| MS.gene46376 | MS.gene056009 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056009.t1 | MTR_1g084020 | 97.444 | 978 | 24 | 1 | 1 | 978 | 1 | 977 | 0.0 | 1951 |
| MS.gene056009.t1 | MTR_1g084030 | 82.667 | 300 | 42 | 5 | 317 | 614 | 60 | 351 | 1.22e-163 | 486 |
| MS.gene056009.t1 | MTR_1g084030 | 50.000 | 88 | 23 | 2 | 1 | 88 | 1 | 67 | 1.24e-15 | 80.9 |
| MS.gene056009.t1 | MTR_4g099020 | 38.472 | 746 | 397 | 13 | 232 | 968 | 121 | 813 | 1.99e-154 | 477 |
| MS.gene056009.t1 | MTR_4g099020 | 38.472 | 746 | 397 | 13 | 232 | 968 | 117 | 809 | 2.99e-154 | 476 |
| MS.gene056009.t1 | MTR_4g099020 | 38.472 | 746 | 397 | 13 | 232 | 968 | 184 | 876 | 6.43e-154 | 478 |
| MS.gene056009.t1 | MTR_5g013790 | 39.252 | 749 | 387 | 16 | 232 | 968 | 179 | 871 | 4.25e-149 | 465 |
| MS.gene056009.t1 | MTR_1g083990 | 91.176 | 204 | 16 | 1 | 769 | 972 | 106 | 307 | 1.15e-123 | 384 |
| MS.gene056009.t1 | MTR_1522s0010 | 30.636 | 173 | 119 | 1 | 430 | 602 | 24 | 195 | 3.99e-20 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056009.t1 | AT5G26860 | 73.286 | 977 | 222 | 6 | 1 | 975 | 46 | 985 | 0.0 | 1428 |
| MS.gene056009.t1 | AT3G05790 | 69.057 | 976 | 247 | 13 | 1 | 969 | 1 | 928 | 0.0 | 1291 |
| MS.gene056009.t1 | AT3G05790 | 68.288 | 987 | 247 | 14 | 1 | 969 | 1 | 939 | 0.0 | 1281 |
| MS.gene056009.t1 | AT3G05780 | 62.023 | 969 | 255 | 12 | 19 | 968 | 46 | 920 | 0.0 | 1155 |
| MS.gene056009.t1 | AT5G47040 | 36.318 | 793 | 437 | 12 | 194 | 968 | 133 | 875 | 2.34e-145 | 455 |
| MS.gene056009.t1 | AT3G05775 | 55.147 | 136 | 39 | 2 | 361 | 496 | 18 | 131 | 3.20e-43 | 154 |
Find 235 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGATATTTACCTTCTTTA+TGG | 0.118362 | 1.3:+59704613 | None:intergenic |
| TATCCAAGGCTAGCAGATTT+TGG | 0.196088 | 1.3:-59705077 | MS.gene056009:CDS |
| CAAACTATTGGCAGCTTTAC+AGG | 0.199476 | 1.3:-59709289 | MS.gene056009:CDS |
| CAGATCTTTGATTTGGCTTT+TGG | 0.207377 | 1.3:-59699234 | MS.gene056009:CDS |
| GAGTCGGAGTCATCGTTATT+TGG | 0.208456 | 1.3:-59710830 | MS.gene056009:CDS |
| CAGAGTCTGGCTCATCTTTA+AGG | 0.209228 | 1.3:+59709226 | None:intergenic |
| GGTACTACTACCATTCAAAA+AGG | 0.243783 | 1.3:-59700739 | MS.gene056009:CDS |
| AAATCTTCTTCAGCTTTCTT+TGG | 0.265036 | 1.3:+59700772 | None:intergenic |
| CTGGTTGACTGCACTGCCTT+GGG | 0.267506 | 1.3:-59703548 | MS.gene056009:CDS |
| AACACCTATTGGCGTTGTTA+TGG | 0.279528 | 1.3:-59700062 | MS.gene056009:CDS |
| TTCTTAAAACAAGTTCTCTT+TGG | 0.287368 | 1.3:-59708525 | MS.gene056009:CDS |
| ACTGGGGAAGTGACACTTAC+AGG | 0.295346 | 1.3:-59699719 | MS.gene056009:CDS |
| CTATATGCAGATCTTTGATT+TGG | 0.296609 | 1.3:-59699241 | MS.gene056009:CDS |
| ATGGAGGTTGTTACTATTGC+TGG | 0.307211 | 1.3:-59701370 | MS.gene056009:CDS |
| CTGTAAAGCTGCCAATAGTT+TGG | 0.307431 | 1.3:+59709290 | None:intergenic |
| AAGAGTGTATATGATTTAAA+AGG | 0.309568 | 1.3:-59709171 | MS.gene056009:CDS |
| TGTAAAGCTGCCAATAGTTT+GGG | 0.309772 | 1.3:+59709291 | None:intergenic |
| ATCCTATCCAATAGGGGATT+TGG | 0.313473 | 1.3:+59701391 | None:intergenic |
| GCACATTGCAAGAGATTATC+TGG | 0.313888 | 1.3:-59701326 | MS.gene056009:CDS |
| ATCTTTGATCAAACACCTAT+TGG | 0.323599 | 1.3:-59700073 | MS.gene056009:CDS |
| ACACCTATTGGCGTTGTTAT+GGG | 0.332720 | 1.3:-59700061 | MS.gene056009:CDS |
| TCATGTTGCGACTTACTCTA+AGG | 0.333632 | 1.3:-59708497 | MS.gene056009:intron |
| GATGGAAATCAGTAAGATTC+AGG | 0.339212 | 1.3:-59704795 | MS.gene056009:intron |
| TTCTGTTCGGAGTCGGAATC+CGG | 0.342934 | 1.3:-59710793 | MS.gene056009:CDS |
| TTGCATATCACCGGTCAATT+AGG | 0.347116 | 1.3:-59699959 | MS.gene056009:CDS |
| CGGAGAACCGGAACCGCGGC+GGG | 0.348037 | 1.3:+59710910 | None:intergenic |
| AGGCATTGTACCATCTTTCC+TGG | 0.351046 | 1.3:+59701972 | None:intergenic |
| GAAAACTATTGCCGAGAATC+AGG | 0.352602 | 1.3:-59701091 | MS.gene056009:CDS |
| AGTCGGAGTCATCGTTATTT+GGG | 0.352726 | 1.3:-59710829 | MS.gene056009:CDS |
| GACGACAAGACAGCACTTAC+AGG | 0.353278 | 1.3:-59704075 | MS.gene056009:intron |
| AACCTCCATCCTATCCAATA+GGG | 0.354645 | 1.3:+59701384 | None:intergenic |
| CCAGCACTTGGTCCATCCTT+TGG | 0.358352 | 1.3:+59699809 | None:intergenic |
| CAGTGTTACCCGTAACTACT+TGG | 0.360213 | 1.3:-59703572 | MS.gene056009:CDS |
| CAACCTCCATCCTATCCAAT+AGG | 0.361697 | 1.3:+59701383 | None:intergenic |
| TGATGTGAAGGAAAGAATAT+TGG | 0.374667 | 1.3:-59703041 | MS.gene056009:CDS |
| ACTGGTTGACTGCACTGCCT+TGG | 0.375558 | 1.3:-59703549 | MS.gene056009:CDS |
| TAGCATTCTGCTCGGGGTCT+AGG | 0.389231 | 1.3:+59701649 | None:intergenic |
| TGGCTGTTCCCACATTCTTA+AGG | 0.390705 | 1.3:+59701952 | None:intergenic |
| CACTGCCTTGGGGTGAATAC+AGG | 0.393770 | 1.3:-59703537 | MS.gene056009:intron |
| AAAATTGGCATGTAAAATCC+TGG | 0.395201 | 1.3:+59709598 | None:intergenic |
| GATGATACCAAATCCCCTAT+TGG | 0.398960 | 1.3:-59701398 | MS.gene056009:CDS |
| GCAGAAGAAGCAGCCTTTGA+CGG | 0.404688 | 1.3:+59710718 | None:intergenic |
| TCGTTAATTGTGCAGCATAT+AGG | 0.409197 | 1.3:-59705110 | MS.gene056009:intron |
| CTTCTTCACAGGCTTCTTCA+AGG | 0.409313 | 1.3:+59699754 | None:intergenic |
| AGCTCTAGCAAAGCACTTGC+TGG | 0.410126 | 1.3:+59701672 | None:intergenic |
| TTGTTCTATTTGCAGGAATT+GGG | 0.411751 | 1.3:-59704108 | MS.gene056009:intron |
| AGGAGGAAGGCACCAGCATA+AGG | 0.414304 | 1.3:+59709246 | None:intergenic |
| CTAGCTCTTCAAGAACTTGT+TGG | 0.416877 | 1.3:+59705021 | None:intergenic |
| TCGGATGCCGGTCGGAGAAC+CGG | 0.421239 | 1.3:+59710898 | None:intergenic |
| CATCCAGGACCCCAAACTAT+TGG | 0.421388 | 1.3:-59709301 | MS.gene056009:intron |
| ATTCTTGATGAAGACCATTA+CGG | 0.422676 | 1.3:-59703070 | MS.gene056009:CDS |
| TGTAAAAGTTGATCACCTTA+AGG | 0.424645 | 1.3:-59708737 | MS.gene056009:intron |
| TAAGACTATAATATTTCCTT+CGG | 0.430379 | 1.3:-59699334 | MS.gene056009:CDS |
| AGTGAGGTAATCTTCAGGTC+TGG | 0.432451 | 1.3:+59710678 | None:intergenic |
| AAAAGAAAGAGAGGAAGATA+AGG | 0.435059 | 1.3:-59700185 | MS.gene056009:CDS |
| CGCGGTTCCGGTTCTCCGAC+CGG | 0.435668 | 1.3:-59710905 | MS.gene056009:CDS |
| TTCTTCCGATTCTCTGTTGG+AGG | 0.437637 | 1.3:-59702139 | MS.gene056009:CDS |
| TCCTTTGGTGTAGCCCCTGC+AGG | 0.441469 | 1.3:+59699824 | None:intergenic |
| CATTACATCTCCTAATTGAC+CGG | 0.444442 | 1.3:+59699949 | None:intergenic |
| GCTGCTCATTTAACAAATAA+CGG | 0.445307 | 1.3:+59704642 | None:intergenic |
| ACTACATTGGGATCAGAGTC+TGG | 0.445698 | 1.3:+59709213 | None:intergenic |
| GGATCCGCCCATTGCAGTCC+AGG | 0.446975 | 1.3:+59700033 | None:intergenic |
| CAAAGATGATGACATCATAA+AGG | 0.451957 | 1.3:-59708584 | MS.gene056009:CDS |
| TTTGTTAAATGAGCAGCTTA+AGG | 0.452656 | 1.3:-59704636 | MS.gene056009:CDS |
| TTTAACCGTCTTCATGAAGT+TGG | 0.454685 | 1.3:-59709138 | MS.gene056009:CDS |
| GGAAGATTCTTCCCATTGGC+GGG | 0.455433 | 1.3:-59699697 | MS.gene056009:CDS |
| GGGCATCGTCGAACATATAT+AGG | 0.456607 | 1.3:-59702002 | MS.gene056009:CDS |
| TTTCTCCCGTAGTATATCTC+TGG | 0.456893 | 1.3:+59699895 | None:intergenic |
| GGACTTGCCTGGACTGCAAT+GGG | 0.458484 | 1.3:-59700040 | MS.gene056009:CDS |
| ATGCGCTCTGCATGGAAGAC+AGG | 0.461919 | 1.3:+59700094 | None:intergenic |
| TCGGAAGAACTTACGGTTCA+AGG | 0.462402 | 1.3:+59702153 | None:intergenic |
| CCAACAGTGAGGTAATCTTC+AGG | 0.462835 | 1.3:+59710673 | None:intergenic |
| GGTTTCTGACTCCTGATTCT+CGG | 0.463551 | 1.3:+59701080 | None:intergenic |
| AGTCCCATAACAACGCCAAT+AGG | 0.466901 | 1.3:+59700058 | None:intergenic |
| TCAACCAGTCCAAGTAGTTA+CGG | 0.467423 | 1.3:+59703563 | None:intergenic |
| ATCAATCAATACAAGAGGAT+TGG | 0.467735 | 1.3:+59701932 | None:intergenic |
| CACATGATCGGGTCCGTCGC+CGG | 0.469622 | 1.3:+59710774 | None:intergenic |
| GTGAGGTAATCTTCAGGTCT+GGG | 0.470829 | 1.3:+59710679 | None:intergenic |
| AGAAAATTAGCATTCTGCTC+GGG | 0.472974 | 1.3:+59701642 | None:intergenic |
| TTTCACCTCCATGTTCCTGC+AGG | 0.474314 | 1.3:-59699839 | MS.gene056009:CDS |
| ACGTCTGCGCATAACAGATA+TGG | 0.474819 | 1.3:-59708946 | MS.gene056009:intron |
| AGCTGATGTGGCTGAAATCA+AGG | 0.477589 | 1.3:-59702113 | MS.gene056009:intron |
| ATCTTCAGGTCTGGGATAAG+TGG | 0.477893 | 1.3:+59710687 | None:intergenic |
| ATGGAGGTGAAACTTTGTAT+TGG | 0.479069 | 1.3:+59699850 | None:intergenic |
| CAGAGAATCGGAAGAACTTA+CGG | 0.479231 | 1.3:+59702146 | None:intergenic |
| GGAGGAAGGCACCAGCATAA+GGG | 0.480370 | 1.3:+59709247 | None:intergenic |
| CGCAAAGCTACAGCTGTTGG+AGG | 0.483633 | 1.3:-59703611 | MS.gene056009:CDS |
| TTAACCGTCTTCATGAAGTT+GGG | 0.483727 | 1.3:-59709137 | MS.gene056009:CDS |
| TTCACCTCCATGTTCCTGCA+GGG | 0.487052 | 1.3:-59699838 | MS.gene056009:CDS |
| CAACCAGTCCAAGTAGTTAC+GGG | 0.489175 | 1.3:+59703564 | None:intergenic |
| GATGAAATGAGCTTCAACAT+TGG | 0.489422 | 1.3:+59710955 | None:intergenic |
| CTATTTGCAGGAATTGGGAC+TGG | 0.490136 | 1.3:-59704103 | MS.gene056009:intron |
| TATTGGAATTCATAGCTGTT+GGG | 0.491082 | 1.3:-59703024 | MS.gene056009:CDS |
| CTTGAAGAAGCCTGTGAAGA+AGG | 0.492602 | 1.3:-59699753 | MS.gene056009:CDS |
| TCTTTCTGGTCCACCAGGCG+TGG | 0.492953 | 1.3:-59702209 | MS.gene056009:CDS |
| ATCTGTCTTTCTGGTCCACC+AGG | 0.496614 | 1.3:-59702214 | MS.gene056009:CDS |
| GGAGATGCGGAGTCGGATGC+CGG | 0.497275 | 1.3:+59710886 | None:intergenic |
| TACCAGATTTCAAGTATCCA+TGG | 0.498537 | 1.3:-59708996 | MS.gene056009:intron |
| AAGAAGGATCTAGCAATGAC+TGG | 0.500187 | 1.3:-59699737 | MS.gene056009:CDS |
| AAGTTCTTCCGATTCTCTGT+TGG | 0.500807 | 1.3:-59702142 | MS.gene056009:CDS |
| GCAGACGTCGATGACCAATC+AGG | 0.501261 | 1.3:+59708961 | None:intergenic |
| TTCAGCATCAACCACATGAT+CGG | 0.501309 | 1.3:+59710762 | None:intergenic |
| GCTCCAAAATCTGCTAGCCT+TGG | 0.502465 | 1.3:+59705074 | None:intergenic |
| TTTCAGGTAAATTCAGGGAA+AGG | 0.503118 | 1.3:-59703693 | MS.gene056009:intron |
| GATCGACAAGCCCCTTATGC+TGG | 0.504477 | 1.3:-59709258 | MS.gene056009:CDS |
| GAGAACCGGAACCGCGGCGG+GGG | 0.505635 | 1.3:+59710912 | None:intergenic |
| GGGACTTGCCTGGACTGCAA+TGG | 0.506970 | 1.3:-59700041 | MS.gene056009:CDS |
| ATTGCTAGATCCTTCTTCAC+AGG | 0.508073 | 1.3:+59699743 | None:intergenic |
| ACTCGCAAAGCTACAGCTGT+TGG | 0.510817 | 1.3:-59703614 | MS.gene056009:CDS |
| GCGTTGTTATGGGACTTGCC+TGG | 0.510909 | 1.3:-59700051 | MS.gene056009:CDS |
| CTAATCCTCCAACAGAGAAT+CGG | 0.510914 | 1.3:+59702134 | None:intergenic |
| GTTGAACAAGGGGACGGAAA+AGG | 0.512423 | 1.3:-59699986 | MS.gene056009:CDS |
| TTCAATTGATAGTTGGGCAA+AGG | 0.515247 | 1.3:-59701708 | MS.gene056009:intron |
| GGGGATCAAGTGATCCTGAT+TGG | 0.515553 | 1.3:-59708975 | MS.gene056009:CDS |
| GTAGGTTACAAAAGAAAGAG+AGG | 0.517536 | 1.3:-59700194 | MS.gene056009:intron |
| AGGTGCTATGCCAGGAAAGA+TGG | 0.518171 | 1.3:-59701982 | MS.gene056009:CDS |
| CGATGGGAACATCAAGATAG+TGG | 0.518244 | 1.3:+59701616 | None:intergenic |
| ATGTAAAATCCTGGGAAAAG+AGG | 0.521237 | 1.3:+59709607 | None:intergenic |
| TCAGCATCAACCACATGATC+GGG | 0.521329 | 1.3:+59710763 | None:intergenic |
| TCAGAGCTAGATACTACATT+GGG | 0.523442 | 1.3:+59709201 | None:intergenic |
| AAATCCCCTATTGGATAGGA+TGG | 0.523871 | 1.3:-59701389 | MS.gene056009:CDS |
| ATATTGGAATTCATAGCTGT+TGG | 0.524360 | 1.3:-59703025 | MS.gene056009:CDS |
| CGATAAATCTACAGATATTG+AGG | 0.524661 | 1.3:-59700611 | MS.gene056009:intron |
| TCGGAGAACCGGAACCGCGG+CGG | 0.525377 | 1.3:+59710909 | None:intergenic |
| TCGAGGTCATATCAACACTA+AGG | 0.526191 | 1.3:-59708552 | MS.gene056009:CDS |
| TCACAGGCTTCTTCAAGGCA+AGG | 0.527647 | 1.3:+59699759 | None:intergenic |
| TTTCAACATACCCCGCCAAT+GGG | 0.528178 | 1.3:+59699686 | None:intergenic |
| GCAATTGAAGAAAAGATAAG+TGG | 0.530216 | 1.3:-59704674 | MS.gene056009:CDS |
| ATTGAAACTTACACTCGAGT+TGG | 0.530852 | 1.3:-59704828 | MS.gene056009:CDS |
| CTAGCACCTAATGTAAAAGA+AGG | 0.533550 | 1.3:-59699288 | MS.gene056009:CDS |
| AGCTGAGGAAACTCCGTCAA+AGG | 0.534058 | 1.3:-59710731 | MS.gene056009:CDS |
| GTGTAGCCCCTGCAGGAACA+TGG | 0.534094 | 1.3:+59699831 | None:intergenic |
| TCACATCAGTTAATCCGTAA+TGG | 0.534207 | 1.3:+59703056 | None:intergenic |
| GGAGAACCGGAACCGCGGCG+GGG | 0.537227 | 1.3:+59710911 | None:intergenic |
| TGGAGGTTGTTACTATTGCT+GGG | 0.538931 | 1.3:-59701369 | MS.gene056009:CDS |
| CATAAAGGCCACATCTTTCG+AGG | 0.539389 | 1.3:-59708569 | MS.gene056009:CDS |
| GGGAAGATTCTTCCCATTGG+CGG | 0.539390 | 1.3:-59699698 | MS.gene056009:CDS |
| TCCTTATTTGCAGGTTAGCG+AGG | 0.540613 | 1.3:-59708770 | MS.gene056009:intron |
| ACACAGAGAGATTGATCTGC+CGG | 0.541557 | 1.3:+59700637 | None:intergenic |
| CAGAAAATTAGCATTCTGCT+CGG | 0.543929 | 1.3:+59701641 | None:intergenic |
| CCAAAGGATGGACCAAGTGC+TGG | 0.544861 | 1.3:-59699809 | MS.gene056009:CDS |
| ACCAACCTTCGATAGATCGA+TGG | 0.546134 | 1.3:+59701599 | None:intergenic |
| AAGAAGCGGAGATGCGGAGT+CGG | 0.548637 | 1.3:+59710879 | None:intergenic |
| TATTGGCCGTTCAATTGCAC+GGG | 0.548721 | 1.3:-59702176 | MS.gene056009:CDS |
| GGTACAATGCCTTAAGAATG+TGG | 0.549700 | 1.3:-59701961 | MS.gene056009:CDS |
| AAATCTCTCTTATTTGCCGA+AGG | 0.551137 | 1.3:+59699318 | None:intergenic |
| CACAGGCTTCTTCAAGGCAA+GGG | 0.551692 | 1.3:+59699760 | None:intergenic |
| ATGAGAACTTTCATGTCACT+CGG | 0.551746 | 1.3:-59703102 | MS.gene056009:CDS |
| ACAGGGAAGATTCTTCCCAT+TGG | 0.553268 | 1.3:-59699701 | MS.gene056009:CDS |
| CTTTCTGGTCCACCAGGCGT+GGG | 0.553340 | 1.3:-59702208 | MS.gene056009:CDS |
| ACCAGATTTCAAGTATCCAT+GGG | 0.554863 | 1.3:-59708995 | MS.gene056009:intron |
| CAAAGCACTTGCTGGATCAC+CGG | 0.556600 | 1.3:+59701680 | None:intergenic |
| CTGGGGAAGTGACACTTACA+GGG | 0.558048 | 1.3:-59699718 | MS.gene056009:CDS |
| AACTCTAAGAAGCGGAGATG+CGG | 0.558089 | 1.3:+59710873 | None:intergenic |
| GAGGAAGGCACCAGCATAAG+GGG | 0.562567 | 1.3:+59709248 | None:intergenic |
| CAATTGCCAGAGATATACTA+CGG | 0.563266 | 1.3:-59699901 | MS.gene056009:CDS |
| GTCATCGTGCAACCAGCACT+TGG | 0.567012 | 1.3:+59699797 | None:intergenic |
| TCACCTCCATGTTCCTGCAG+GGG | 0.567880 | 1.3:-59699837 | MS.gene056009:CDS |
| TGAAGCATGTGGAATAAAGC+CGG | 0.570845 | 1.3:-59701290 | MS.gene056009:CDS |
| GAAAATTAGCATTCTGCTCG+GGG | 0.572122 | 1.3:+59701643 | None:intergenic |
| GCAGCTTAAGGCCATAAAGA+AGG | 0.572516 | 1.3:-59704624 | MS.gene056009:intron |
| TCCTGCAGGGGCTACACCAA+AGG | 0.573234 | 1.3:-59699825 | MS.gene056009:CDS |
| GACTAAATCTGTTGAACAAG+GGG | 0.573898 | 1.3:-59699996 | MS.gene056009:CDS |
| CGAGTTGGTAAAGAAAGAGA+TGG | 0.574235 | 1.3:-59704813 | MS.gene056009:CDS |
| TGGTTGACTGCACTGCCTTG+GGG | 0.574302 | 1.3:-59703547 | MS.gene056009:CDS |
| ATTTCATCAATCAATACAAG+AGG | 0.574893 | 1.3:+59701927 | None:intergenic |
| CGGAGTCATCGTTATTTGGG+AGG | 0.577310 | 1.3:-59710826 | MS.gene056009:CDS |
| CTTGCCTGGACTGCAATGGG+CGG | 0.579469 | 1.3:-59700037 | MS.gene056009:CDS |
| GTATTGGCCGTTCAATTGCA+CGG | 0.579673 | 1.3:-59702177 | MS.gene056009:CDS |
| TGGCTCATCTTTAAGGAGGA+AGG | 0.579983 | 1.3:+59709233 | None:intergenic |
| ACATATATAGGTGCTATGCC+AGG | 0.581505 | 1.3:-59701990 | MS.gene056009:CDS |
| AGAAAACTATTGCTGCAAGA+AGG | 0.583435 | 1.3:-59699365 | MS.gene056009:CDS |
| TCAAGGCCCGTGCAATTGAA+CGG | 0.585038 | 1.3:+59702170 | None:intergenic |
| AAATTGGCATGTAAAATCCT+GGG | 0.585606 | 1.3:+59709599 | None:intergenic |
| AGAAGGATCTAGCAATGACT+GGG | 0.587164 | 1.3:-59699736 | MS.gene056009:CDS |
| TTACCCGTAACTACTTGGAC+TGG | 0.587817 | 1.3:-59703567 | MS.gene056009:CDS |
| ATAGCTGTTGGGAAATTGAG+AGG | 0.587899 | 1.3:-59703013 | MS.gene056009:CDS |
| TGTTGGAGGATTAGCTGATG+TGG | 0.590169 | 1.3:-59702125 | MS.gene056009:CDS |
| TGCTGAAGCTAAAGCAGCTG+AGG | 0.591116 | 1.3:-59710746 | MS.gene056009:CDS |
| GGAGTCATCGTTATTTGGGA+GGG | 0.592287 | 1.3:-59710825 | MS.gene056009:CDS |
| ATAACTTGTAACACATGAGG+TGG | 0.593186 | 1.3:+59703646 | None:intergenic |
| CGAGGTCATATCAACACTAA+GGG | 0.595033 | 1.3:-59708551 | MS.gene056009:CDS |
| AAAGGAGCATTGCATATCAC+CGG | 0.595784 | 1.3:-59699968 | MS.gene056009:CDS |
| GATCAAAGATGCGCTCTGCA+TGG | 0.595860 | 1.3:+59700086 | None:intergenic |
| GAATTCAAAACTCTAAGAAG+CGG | 0.598158 | 1.3:+59710865 | None:intergenic |
| TAGCTGTTGGGAAATTGAGA+GGG | 0.598517 | 1.3:-59703012 | MS.gene056009:CDS |
| TCAGGATCACTTGATCCCCA+TGG | 0.598561 | 1.3:+59708979 | None:intergenic |
| TTACGGATTAACTGATGTGA+AGG | 0.600376 | 1.3:-59703053 | MS.gene056009:CDS |
| TCGGAGTCGGAATCCGGCGA+CGG | 0.603037 | 1.3:-59710787 | MS.gene056009:CDS |
| CCAACCTTCGATAGATCGAT+GGG | 0.604425 | 1.3:+59701600 | None:intergenic |
| GAAGATTCTTCCCATTGGCG+GGG | 0.605198 | 1.3:-59699696 | MS.gene056009:intron |
| TGAATCGAAGACTGTTGAGA+AGG | 0.606498 | 1.3:-59700158 | MS.gene056009:CDS |
| GGTTAAGCGGTGGATGCAAG+AGG | 0.609761 | 1.3:+59710933 | None:intergenic |
| ATTCTCTCAGCGGATTGAGT+CGG | 0.610901 | 1.3:-59710846 | MS.gene056009:CDS |
| GTCAGAGCTAGATACTACAT+TGG | 0.612372 | 1.3:+59709200 | None:intergenic |
| TACCAAATCCCCTATTGGAT+AGG | 0.612747 | 1.3:-59701393 | MS.gene056009:CDS |
| GAGACTAAATCTGTTGAACA+AGG | 0.612839 | 1.3:-59699998 | MS.gene056009:CDS |
| CGGCGACGGACCCGATCATG+TGG | 0.613445 | 1.3:-59710773 | MS.gene056009:CDS |
| GCAGGGGCTACACCAAAGGA+TGG | 0.614871 | 1.3:-59699821 | MS.gene056009:CDS |
| AAAAGAGGTCTATGTTGCAA+CGG | 0.615327 | 1.3:+59709622 | None:intergenic |
| TTGCTACTCTCTATCAACAC+GGG | 0.615994 | 1.3:+59700712 | None:intergenic |
| TGTTTGCACTGCAAATGTAG+TGG | 0.616187 | 1.3:-59701422 | MS.gene056009:CDS |
| GAGTCCCAACTTCATGAAGA+CGG | 0.618939 | 1.3:+59709133 | None:intergenic |
| ACCTCCATCCTATCCAATAG+GGG | 0.626225 | 1.3:+59701385 | None:intergenic |
| GTACAATGCCTTAAGAATGT+GGG | 0.627193 | 1.3:-59701960 | MS.gene056009:CDS |
| GGCAAGGGACAACATAGAAG+TGG | 0.633373 | 1.3:+59699775 | None:intergenic |
| ATGCGGAGTCGGATGCCGGT+CGG | 0.634475 | 1.3:+59710890 | None:intergenic |
| TAGTTGGGCAAAGGACATGC+CGG | 0.635078 | 1.3:-59701699 | MS.gene056009:CDS |
| TCCCCTATTGGATAGGATGG+AGG | 0.636687 | 1.3:-59701386 | MS.gene056009:CDS |
| TGTTCCCATCGATCTATCGA+AGG | 0.639058 | 1.3:-59701604 | MS.gene056009:intron |
| AAAACTACACGTGAAGCATG+TGG | 0.641017 | 1.3:-59701301 | MS.gene056009:CDS |
| TGAGATGAACACAAATCCAC+CGG | 0.643292 | 1.3:-59700656 | MS.gene056009:CDS |
| AAATTGAGAGGGACTTCACA+AGG | 0.643864 | 1.3:-59703001 | MS.gene056009:intron |
| AAATCTGTTGAACAAGGGGA+CGG | 0.651170 | 1.3:-59699992 | MS.gene056009:CDS |
| AGACTAAATCTGTTGAACAA+GGG | 0.652455 | 1.3:-59699997 | MS.gene056009:CDS |
| TATTGCTGCAAGAAGGAGTG+AGG | 0.652496 | 1.3:-59699358 | MS.gene056009:CDS |
| GTAAAGCTGCCAATAGTTTG+GGG | 0.653963 | 1.3:+59709292 | None:intergenic |
| TCTATAACTTGTAACACATG+AGG | 0.655916 | 1.3:+59703643 | None:intergenic |
| TTTAGTCTCTATATACAGTG+TGG | 0.656615 | 1.3:+59700012 | None:intergenic |
| CGGTCGGAGAACCGGAACCG+CGG | 0.661798 | 1.3:+59710906 | None:intergenic |
| TAACTTGTAACACATGAGGT+GGG | 0.663798 | 1.3:+59703647 | None:intergenic |
| ATTGCTACTCTCTATCAACA+CGG | 0.665388 | 1.3:+59700711 | None:intergenic |
| AATTGCCAGAGATATACTAC+GGG | 0.666821 | 1.3:-59699900 | MS.gene056009:CDS |
| AGATAGCGCTACGACTTGTG+AGG | 0.667785 | 1.3:-59700825 | MS.gene056009:CDS |
| TGAGAACTTTCATGTCACTC+GGG | 0.669113 | 1.3:-59703101 | MS.gene056009:CDS |
| CAGAGAGATTGATCTGCCGG+TGG | 0.680139 | 1.3:+59700640 | None:intergenic |
| AGTCTGGCTCATCTTTAAGG+AGG | 0.681007 | 1.3:+59709229 | None:intergenic |
| ACAAACCTGTATTCACCCCA+AGG | 0.685382 | 1.3:+59703532 | None:intergenic |
| TGATATGACCTCGAAAGATG+TGG | 0.692394 | 1.3:+59708561 | None:intergenic |
| TGCTACTCTCTATCAACACG+GGG | 0.697572 | 1.3:+59700713 | None:intergenic |
| GCTACGACTTGTGAGGCACG+TGG | 0.709175 | 1.3:-59700818 | MS.gene056009:CDS |
| CCAGATTTCAAGTATCCATG+GGG | 0.727231 | 1.3:-59708994 | MS.gene056009:intron |
| GAAGGATCTAGCAATGACTG+GGG | 0.732838 | 1.3:-59699735 | MS.gene056009:CDS |
| TAGCCCCTGCAGGAACATGG+AGG | 0.733655 | 1.3:+59699834 | None:intergenic |
| TGTGTTACAAGTTATAGACG+AGG | 0.751251 | 1.3:-59703638 | MS.gene056009:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 59699203 | 59710974 | 59699203 | ID=MS.gene056009 |
| chr1.3 | mRNA | 59699203 | 59710974 | 59699203 | ID=MS.gene056009.t1;Parent=MS.gene056009 |
| chr1.3 | exon | 59710678 | 59710974 | 59710678 | ID=MS.gene056009.t1.exon1;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59710678 | 59710974 | 59710678 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59709591 | 59709656 | 59709591 | ID=MS.gene056009.t1.exon2;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59709591 | 59709656 | 59709591 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59709125 | 59709316 | 59709125 | ID=MS.gene056009.t1.exon3;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59709125 | 59709316 | 59709125 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59708947 | 59709012 | 59708947 | ID=MS.gene056009.t1.exon4;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59708947 | 59709012 | 59708947 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59708738 | 59708779 | 59708738 | ID=MS.gene056009.t1.exon5;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59708738 | 59708779 | 59708738 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59708498 | 59708620 | 59708498 | ID=MS.gene056009.t1.exon6;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59708498 | 59708620 | 59708498 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59705019 | 59705117 | 59705019 | ID=MS.gene056009.t1.exon7;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59705019 | 59705117 | 59705019 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59704796 | 59704861 | 59704796 | ID=MS.gene056009.t1.exon8;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59704796 | 59704861 | 59704796 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59704625 | 59704711 | 59704625 | ID=MS.gene056009.t1.exon9;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59704625 | 59704711 | 59704625 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59704076 | 59704115 | 59704076 | ID=MS.gene056009.t1.exon10;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59704076 | 59704115 | 59704076 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59703538 | 59703709 | 59703538 | ID=MS.gene056009.t1.exon11;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59703538 | 59703709 | 59703538 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59703002 | 59703126 | 59703002 | ID=MS.gene056009.t1.exon12;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59703002 | 59703126 | 59703002 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59702114 | 59702244 | 59702114 | ID=MS.gene056009.t1.exon13;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59702114 | 59702244 | 59702114 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59701920 | 59702024 | 59701920 | ID=MS.gene056009.t1.exon14;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59701920 | 59702024 | 59701920 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59701605 | 59701718 | 59701605 | ID=MS.gene056009.t1.exon15;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59701605 | 59701718 | 59701605 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59701285 | 59701452 | 59701285 | ID=MS.gene056009.t1.exon16;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59701285 | 59701452 | 59701285 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59701048 | 59701149 | 59701048 | ID=MS.gene056009.t1.exon17;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59701048 | 59701149 | 59701048 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59700612 | 59700845 | 59700612 | ID=MS.gene056009.t1.exon18;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59700612 | 59700845 | 59700612 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59699697 | 59700212 | 59699697 | ID=MS.gene056009.t1.exon19;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59699697 | 59700212 | 59699697 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| chr1.3 | exon | 59699203 | 59699394 | 59699203 | ID=MS.gene056009.t1.exon20;Parent=MS.gene056009.t1 |
| chr1.3 | CDS | 59699203 | 59699394 | 59699203 | ID=cds.MS.gene056009.t1;Parent=MS.gene056009.t1 |
| Gene Sequence |
| Protein sequence |
