AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05622


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05622.t1 MTR_7g029510 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_7g099610 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_5g063620 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_4g061827 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_4g061920 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g462400 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g054380 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g462730 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g462510 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g070920 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g061440 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_3g462410 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_2g096100 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_8g038460 100.000 101 0 0 26 126 3 103 2.09e-67 198
MS.gene05622.t1 MTR_4g128150 100.000 100 0 0 26 125 3 102 1.31e-66 206
MS.gene05622.t1 MTR_7g029520 84.000 50 8 0 50 99 1 50 6.53e-21 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05622.t1 AT5G59970 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT5G59690 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT5G59970 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT3G46320 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT3G45930 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT3G53730 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT2G28740 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT1G07660 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT1G07820 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT1G07820 100.000 101 0 0 26 126 3 103 2.19e-67 198
MS.gene05622.t1 AT1G07660 83.168 101 0 1 26 126 3 86 4.66e-50 154

Find 45 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCCTGTGCGGTTGTGTAC+TGG 0.281117 3.2:-53428249 MS.gene05622:CDS
GGAAGAACCCTCTATGGTTT+TGG 0.304646 3.2:-53427421 MS.gene05622:CDS
AGGTGTCTTGAAGATCTTCT+TGG 0.396647 3.2:-53427536 MS.gene05622:CDS
GCACGGCGAGCAAGCGGCGG+AGG 0.402136 3.2:+53428295 None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG 0.408859 3.2:-53427706 MS.gene05622:intron
GTCCTGTGCGGTTGTGTACT+GGG 0.424332 3.2:-53428248 MS.gene05622:intron
TTCAACCTCCAAAACCATAG+AGG 0.438439 3.2:+53427413 None:intergenic
GACAAGGGCGACGGCGAGAT+GGG 0.442793 3.2:+53428268 None:intergenic
AGGCAGGGAAGAACCCTCTA+TGG 0.447332 3.2:-53427427 MS.gene05622:CDS
CTCGCCAATCGGCGAATAGC+AGG 0.453484 3.2:+53427607 None:intergenic
GGAGCACGGCGAGCAAGCGG+CGG 0.454610 3.2:+53428292 None:intergenic
GGTAAGGGTCTGGGAAAGGG+AGG 0.476615 3.2:-53427682 MS.gene05622:CDS
GGCGGAGCACGGCGAGCAAG+CGG 0.481094 3.2:+53428289 None:intergenic
TGTCTATGCTTTGAAGAGGC+AGG 0.485721 3.2:-53427443 MS.gene05622:CDS
GGACAAGGGCGACGGCGAGA+TGG 0.496013 3.2:+53428267 None:intergenic
CACCACCTCTTCTCGCCAAT+CGG 0.499060 3.2:+53427596 None:intergenic
GGTTCTTCGTGACAACATCC+AGG 0.501637 3.2:-53427641 MS.gene05622:CDS
CAAGCCTGCTATTCGCCGAT+TGG 0.502152 3.2:-53427611 MS.gene05622:CDS
CAATCGGCGAATAGCAGGCT+TGG 0.515998 3.2:+53427612 None:intergenic
AGGGAGGTGCAAAGAGACAT+AGG 0.520046 3.2:-53427666 MS.gene05622:CDS
TTACATACACTGAGCATGCT+AGG 0.520723 3.2:-53427492 MS.gene05622:CDS
CGCCGATTGGCGAGAAGAGG+TGG 0.530277 3.2:-53427598 MS.gene05622:CDS
GTCTATGCTTTGAAGAGGCA+GGG 0.532756 3.2:-53427442 MS.gene05622:CDS
ACCGCACAGGACAAGGGCGA+CGG 0.537076 3.2:+53428259 None:intergenic
ACGGCGAGATGGGCGGAGCA+CGG 0.538504 3.2:+53428278 None:intergenic
AAGGGCGACGGCGAGATGGG+CGG 0.546356 3.2:+53428271 None:intergenic
GAAAATGTCAGGCCGTGGAA+AGG 0.547267 3.2:-53427707 MS.gene05622:intron
AATGTCAGGCCGTGGAAAGG+GGG 0.551839 3.2:-53427704 MS.gene05622:intron
TACCCAGTACACAACCGCAC+AGG 0.564180 3.2:+53428246 None:intergenic
AAATGTCAGGCCGTGGAAAG+GGG 0.566511 3.2:-53427705 MS.gene05622:intron
GAGGAAGACTGTGACAGCCA+TGG 0.570644 3.2:-53427470 MS.gene05622:CDS
TCAACCTCCAAAACCATAGA+GGG 0.578325 3.2:+53427414 None:intergenic
GTACACAACCGCACAGGACA+AGG 0.583355 3.2:+53428252 None:intergenic
TAGCAGGCTTGGTGATACCC+TGG 0.594313 3.2:+53427623 None:intergenic
ATGTCAGGCCGTGGAAAGGG+GGG 0.596640 3.2:-53427703 MS.gene05622:intron
GGTGGTGTCAAGAGAATCAG+TGG 0.607763 3.2:-53427580 MS.gene05622:CDS
ATTCGCCGATTGGCGAGAAG+AGG 0.615284 3.2:-53427601 MS.gene05622:CDS
AGGTGCAAAGAGACATAGGA+AGG 0.617656 3.2:-53427662 MS.gene05622:CDS
TTGATATATGAAGAAACAAG+AGG 0.619131 3.2:-53427556 MS.gene05622:CDS
CAAAGCATAGACAACATCCA+TGG 0.629842 3.2:+53427453 None:intergenic
TACACAACCGCACAGGACAA+GGG 0.643330 3.2:+53428253 None:intergenic
GCCGTCGCCCTTGTCCTGTG+CGG 0.664678 3.2:-53428260 MS.gene05622:CDS
GTTCTTCGTGACAACATCCA+GGG 0.676380 3.2:-53427640 MS.gene05622:CDS
ATGTTGTCTATGCTTTGAAG+AGG 0.684273 3.2:-53427447 MS.gene05622:CDS
CATACACTGAGCATGCTAGG+AGG 0.707859 3.2:-53427489 MS.gene05622:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGACTTATGAAATAAAAA+AGG + chr3.2:53427675-53427694 None:intergenic 15.0%
!! AAGACTTATGAAATAAAAAA+GGG + chr3.2:53427674-53427693 None:intergenic 15.0%
!!! CTTTGTATTTTAATAAAAAC+AGG - chr3.2:53427746-53427765 MS.gene05622:intron 15.0%
!! TTGAGAAATTAGAGAAATTA+GGG + chr3.2:53427956-53427975 None:intergenic 20.0%
!! TTTGAGAAATTAGAGAAATT+AGG + chr3.2:53427957-53427976 None:intergenic 20.0%
!!! TATGAAATAAAAAAGGGTAT+TGG + chr3.2:53427668-53427687 None:intergenic 20.0%
! AGATACTAGAGAATATAATG+TGG - chr3.2:53427599-53427618 MS.gene05622:CDS 25.0%
! ATTAAGACATTAGAATGAAC+AGG + chr3.2:53427531-53427550 None:intergenic 25.0%
! CAAAAATTCATTCAATGTGT+TGG + chr3.2:53427579-53427598 None:intergenic 25.0%
! CATGAACAAGGAAATTAAAA+TGG + chr3.2:53427635-53427654 None:intergenic 25.0%
! TTGATATATGAAGAAACAAG+AGG - chr3.2:53428160-53428179 MS.gene05622:intron 25.0%
!!! TTTCATCTTTTGAAAATGTC+AGG - chr3.2:53427998-53428017 MS.gene05622:intron 25.0%
AAACCAAGAGATTAATCCAA+TGG - chr3.2:53427790-53427809 MS.gene05622:intron 30.0%
ACCAAAAAGTTACTGAATCC+AGG - chr3.2:53427845-53427864 MS.gene05622:intron 35.0%
CTTTGTTCAATATCCACCAT+TGG + chr3.2:53427809-53427828 None:intergenic 35.0%
GAGTATTTATCTGTGGTAGT+TGG + chr3.2:53427892-53427911 None:intergenic 35.0%
! ACCTGGATTCAGTAACTTTT+TGG + chr3.2:53427849-53427868 None:intergenic 35.0%
! TGGATTCAGTAACTTTTTGG+TGG + chr3.2:53427846-53427865 None:intergenic 35.0%
!! ATGTTGTCTATGCTTTGAAG+AGG - chr3.2:53428269-53428288 MS.gene05622:CDS 35.0%
!! GGATTCAGTAACTTTTTGGT+GGG + chr3.2:53427845-53427864 None:intergenic 35.0%
AGGTGTCTTGAAGATCTTCT+TGG - chr3.2:53428180-53428199 MS.gene05622:intron 40.0%
ATGTGAGCGAGAAAAGATGA+AGG + chr3.2:53427921-53427940 None:intergenic 40.0%
CAAAGCATAGACAACATCCA+TGG + chr3.2:53428266-53428285 None:intergenic 40.0%
CCAAGAGATTAATCCAATGG+TGG - chr3.2:53427793-53427812 MS.gene05622:intron 40.0%
CCACCATTGGATTAATCTCT+TGG + chr3.2:53427796-53427815 None:intergenic 40.0%
GGGATCTGAGTATTTATCTG+TGG + chr3.2:53427899-53427918 None:intergenic 40.0%
TCAACCTCCAAAACCATAGA+GGG + chr3.2:53428305-53428324 None:intergenic 40.0%
TGTGAGCGAGAAAAGATGAA+GGG + chr3.2:53427920-53427939 None:intergenic 40.0%
TTACATACACTGAGCATGCT+AGG - chr3.2:53428224-53428243 MS.gene05622:intron 40.0%
AAAATGTCAGGCCGTGGAAA+GGG - chr3.2:53428010-53428029 MS.gene05622:intron 45.0%
AGGTGCAAAGAGACATAGGA+AGG - chr3.2:53428054-53428073 MS.gene05622:intron 45.0%
GTGAGCGAGAAAAGATGAAG+GGG + chr3.2:53427919-53427938 None:intergenic 45.0%
GTTCTTCGTGACAACATCCA+GGG - chr3.2:53428076-53428095 MS.gene05622:intron 45.0%
TTTCGTGTACTGTGCAAGCA+TGG - chr3.2:53427702-53427721 MS.gene05622:intron 45.0%
! GGAAGAACCCTCTATGGTTT+TGG - chr3.2:53428295-53428314 MS.gene05622:CDS 45.0%
!! CTTTTGAAAATGTCAGGCCG+TGG - chr3.2:53428004-53428023 MS.gene05622:intron 45.0%
!! GGCATTGACAAGCATGAACA+AGG + chr3.2:53427647-53427666 None:intergenic 45.0%
!! GTCTATGCTTTGAAGAGGCA+GGG - chr3.2:53428274-53428293 MS.gene05622:CDS 45.0%
!! TGTCTATGCTTTGAAGAGGC+AGG - chr3.2:53428273-53428292 MS.gene05622:CDS 45.0%
!!! AGAACCCTCTATGGTTTTGG+AGG - chr3.2:53428298-53428317 MS.gene05622:CDS 45.0%
AAATGTCAGGCCGTGGAAAG+GGG - chr3.2:53428011-53428030 MS.gene05622:intron 50.0%
CATACACTGAGCATGCTAGG+AGG - chr3.2:53428227-53428246 MS.gene05622:intron 50.0%
GAAAATGTCAGGCCGTGGAA+AGG - chr3.2:53428009-53428028 MS.gene05622:intron 50.0%
GGTTCTTCGTGACAACATCC+AGG - chr3.2:53428075-53428094 MS.gene05622:intron 50.0%
TACACAACCGCACAGGACAA+GGG + chr3.2:53427466-53427485 None:intergenic 50.0%
! AGGGAGGTGCAAAGAGACAT+AGG - chr3.2:53428050-53428069 MS.gene05622:intron 50.0%
! GGTGGTGTCAAGAGAATCAG+TGG - chr3.2:53428136-53428155 MS.gene05622:intron 50.0%
AATGTCAGGCCGTGGAAAGG+GGG - chr3.2:53428012-53428031 MS.gene05622:intron 55.0%
AGGCAGGGAAGAACCCTCTA+TGG - chr3.2:53428289-53428308 MS.gene05622:CDS 55.0%
CAAGCCTGCTATTCGCCGAT+TGG - chr3.2:53428105-53428124 MS.gene05622:intron 55.0%
CAATCGGCGAATAGCAGGCT+TGG + chr3.2:53428107-53428126 None:intergenic 55.0%
CACCACCTCTTCTCGCCAAT+CGG + chr3.2:53428123-53428142 None:intergenic 55.0%
GAGGAAGACTGTGACAGCCA+TGG - chr3.2:53428246-53428265 MS.gene05622:intron 55.0%
GTACACAACCGCACAGGACA+AGG + chr3.2:53427467-53427486 None:intergenic 55.0%
GTCCTGTGCGGTTGTGTACT+GGG - chr3.2:53427468-53427487 MS.gene05622:CDS 55.0%
TACCCAGTACACAACCGCAC+AGG + chr3.2:53427473-53427492 None:intergenic 55.0%
TGTCCTGTGCGGTTGTGTAC+TGG - chr3.2:53427467-53427486 MS.gene05622:CDS 55.0%
! ATTCGCCGATTGGCGAGAAG+AGG - chr3.2:53428115-53428134 MS.gene05622:intron 55.0%
!! GTGGTAGTTGGTAGCGAGAG+CGG + chr3.2:53427880-53427899 None:intergenic 55.0%
!! TAGCAGGCTTGGTGATACCC+TGG + chr3.2:53428096-53428115 None:intergenic 55.0%
!! TGGTAGTTGGTAGCGAGAGC+GGG + chr3.2:53427879-53427898 None:intergenic 55.0%
ATGTCAGGCCGTGGAAAGGG+GGG - chr3.2:53428013-53428032 MS.gene05622:intron 60.0%
CTCGCCAATCGGCGAATAGC+AGG + chr3.2:53428112-53428131 None:intergenic 60.0%
GACCCTTACCCCCCTTTCCA+CGG + chr3.2:53428024-53428043 None:intergenic 60.0%
GGAAAGGGGGGTAAGGGTCT+GGG - chr3.2:53428025-53428044 MS.gene05622:intron 60.0%
GGGGGTAAGGGTCTGGGAAA+GGG - chr3.2:53428031-53428050 MS.gene05622:intron 60.0%
GGTAAGGGTCTGGGAAAGGG+AGG - chr3.2:53428034-53428053 MS.gene05622:intron 60.0%
TGGAAAGGGGGGTAAGGGTC+TGG - chr3.2:53428024-53428043 MS.gene05622:intron 60.0%
! TGGTAGCGAGAGCGGGATGA+AGG + chr3.2:53427872-53427891 None:intergenic 60.0%
ACCGCACAGGACAAGGGCGA+CGG + chr3.2:53427460-53427479 None:intergenic 65.0%
AGGCCGTGGAAAGGGGGGTA+AGG - chr3.2:53428018-53428037 MS.gene05622:intron 65.0%
CGAGAGCGGGATGAAGGACC+TGG + chr3.2:53427866-53427885 None:intergenic 65.0%
GACAAGGGCGACGGCGAGAT+GGG + chr3.2:53427451-53427470 None:intergenic 65.0%
GGCCGTGGAAAGGGGGGTAA+GGG - chr3.2:53428019-53428038 MS.gene05622:intron 65.0%
GGGGGGTAAGGGTCTGGGAA+AGG - chr3.2:53428030-53428049 MS.gene05622:intron 65.0%
! CGCCGATTGGCGAGAAGAGG+TGG - chr3.2:53428118-53428137 MS.gene05622:intron 65.0%
AAGGGCGACGGCGAGATGGG+CGG + chr3.2:53427448-53427467 None:intergenic 70.0%
ACGGCGAGATGGGCGGAGCA+CGG + chr3.2:53427441-53427460 None:intergenic 70.0%
GCCGTCGCCCTTGTCCTGTG+CGG - chr3.2:53427456-53427475 MS.gene05622:CDS 70.0%
GGACAAGGGCGACGGCGAGA+TGG + chr3.2:53427452-53427471 None:intergenic 70.0%
GGAGCACGGCGAGCAAGCGG+CGG + chr3.2:53427427-53427446 None:intergenic 75.0%
GGCGGAGCACGGCGAGCAAG+CGG + chr3.2:53427430-53427449 None:intergenic 75.0%
! GCACGGCGAGCAAGCGGCGG+AGG + chr3.2:53427424-53427443 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 53427414 53428324 53427414 ID=MS.gene05622
chr3.2 mRNA 53427414 53428324 53427414 ID=MS.gene05622.t1;Parent=MS.gene05622
chr3.2 exon 53428249 53428324 53428249 ID=MS.gene05622.t1.exon1;Parent=MS.gene05622.t1
chr3.2 CDS 53428249 53428324 53428249 ID=cds.MS.gene05622.t1;Parent=MS.gene05622.t1
chr3.2 exon 53427414 53427718 53427414 ID=MS.gene05622.t1.exon2;Parent=MS.gene05622.t1
chr3.2 CDS 53427414 53427718 53427414 ID=cds.MS.gene05622.t1;Parent=MS.gene05622.t1
Gene Sequence

>MS.gene05622

ATGTCTACCTCCGCCGCTTGCTCGCCGTGCTCCGCCCATCTCGCCGTCGCCCTTGTCCTGTGCGGTTGTGTACTGGGTAAAAATCACAGCTTACTGATATTTAAAAATAAAACTCCTGTTCATTCTAATGTCTTAATTTTTCTTCTGTAAAGATGCTTCTATCCAACACATTGAATGAATTTTTGAGATACTAGAGAATATAATGTGGAAATTCATAACCATTTTAATTTCCTTGTTCATGCTTGTCAATGCCAATACCCTTTTTTATTTCATAAGTCTTTGTATTTCTTTCGTGTACTGTGCAAGCATGGTTGTTTTCAATTTGATTTTTACTTTGTATTTTAATAAAAACAGGTTAGAACATGTGTTAAAATTGAAACCAAGAGATTAATCCAATGGTGGATATTGAACAAAGTCACATAACTTTTCCCACCAAAAAGTTACTGAATCCAGGTCCTTCATCCCGCTCTCGCTACCAACTACCACAGATAAATACTCAGATCCCCTTCATCTTTTCTCGCTCACATTCAAATTCAAAACCCTAATTTCTCTAATTTCTCAAATTTCTTTTTTGTTTGTCGAATTTTCATCTTTTGAAAATGTCAGGCCGTGGAAAGGGGGGTAAGGGTCTGGGAAAGGGAGGTGCAAAGAGACATAGGAAGGTTCTTCGTGACAACATCCAGGGTATCACCAAGCCTGCTATTCGCCGATTGGCGAGAAGAGGTGGTGTCAAGAGAATCAGTGGATTGATATATGAAGAAACAAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACTGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGGCAGGGAAGAACCCTCTATGGTTTTGGAGGTTGA

Protein sequence

>MS.gene05622.t1

MSTSAACSPCSAHLAVALVLCGCVLGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG