AlfalfaGEDB | MS.gene061867

M. sativa cultivar XinJiangDaYe / MS.gene061867

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061867.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061867.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061867.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05622	MS.gene061867	0.819809	9.56E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene89487	MS.gene061867	PPI
MS.gene04392	MS.gene061867	PPI
MS.gene30920	MS.gene061867	PPI
MS.gene08053	MS.gene061867	PPI
MS.gene061872	MS.gene061867	PPI
MS.gene061867	MS.gene018578	PPI
MS.gene04068	MS.gene061867	PPI
MS.gene061867	MS.gene018580	PPI
MS.gene061867	MS.gene004124	PPI
MS.gene070241	MS.gene061867	PPI
MS.gene04434	MS.gene061867	PPI
MS.gene022946	MS.gene061867	PPI
MS.gene04064	MS.gene061867	PPI
MS.gene061876	MS.gene061867	PPI
MS.gene05619	MS.gene061867	PPI
MS.gene05608	MS.gene061867	PPI
MS.gene022932	MS.gene061867	PPI
MS.gene012391	MS.gene061867	PPI
MS.gene88681	MS.gene061867	PPI
MS.gene061867	MS.gene64650	PPI
MS.gene070244	MS.gene061867	PPI
MS.gene035897	MS.gene061867	PPI
MS.gene061867	MS.gene061874	PPI
MS.gene64920	MS.gene061867	PPI
MS.gene060463	MS.gene061867	PPI
MS.gene061867	MS.gene061869	PPI
MS.gene070907	MS.gene061867	PPI
MS.gene009027	MS.gene061867	PPI
MS.gene31196	MS.gene061867	PPI
MS.gene061867	MS.gene04972	PPI
MS.gene010219	MS.gene061867	PPI
MS.gene061867	MS.gene004121	PPI
MS.gene060465	MS.gene061867	PPI
MS.gene061867	MS.gene05619	PPI
MS.gene08121	MS.gene061867	PPI
MS.gene061870	MS.gene061867	PPI
MS.gene061867	MS.gene89488	PPI
MS.gene035718	MS.gene061867	PPI
MS.gene035896	MS.gene061867	PPI
MS.gene73136	MS.gene061867	PPI
MS.gene008276	MS.gene061867	PPI
MS.gene09873	MS.gene061867	PPI
MS.gene78578	MS.gene061867	PPI
MS.gene035721	MS.gene061867	PPI
MS.gene04052	MS.gene061867	PPI
MS.gene08122	MS.gene061867	PPI
MS.gene048705	MS.gene061867	PPI
MS.gene05623	MS.gene061867	PPI
MS.gene043967	MS.gene061867	PPI
MS.gene84536	MS.gene061867	PPI
MS.gene028629	MS.gene061867	PPI
MS.gene061867	MS.gene016624	PPI
MS.gene82546	MS.gene061867	PPI
MS.gene61017	MS.gene061867	PPI
MS.gene04433	MS.gene061867	PPI
MS.gene035787	MS.gene061867	PPI
MS.gene04067	MS.gene061867	PPI
MS.gene71215	MS.gene061867	PPI
MS.gene061866	MS.gene061867	PPI
MS.gene035719	MS.gene061867	PPI
MS.gene58854	MS.gene061867	PPI
MS.gene66225	MS.gene061867	PPI
MS.gene70955	MS.gene061867	PPI
MS.gene071136	MS.gene061867	PPI
MS.gene040383	MS.gene061867	PPI
MS.gene070242	MS.gene061867	PPI
MS.gene072493	MS.gene061867	PPI
MS.gene035720	MS.gene061867	PPI
MS.gene012390	MS.gene061867	PPI
MS.gene048746	MS.gene061867	PPI
MS.gene010220	MS.gene061867	PPI
MS.gene061867	MS.gene049705	PPI
MS.gene79195	MS.gene061867	PPI
MS.gene88680	MS.gene061867	PPI
MS.gene056215	MS.gene061867	PPI
MS.gene061869	MS.gene061867	PPI
MS.gene064557	MS.gene061867	PPI
MS.gene049821	MS.gene061867	PPI
MS.gene62520	MS.gene061867	PPI
MS.gene061867	MS.gene68545	PPI
MS.gene038469	MS.gene061867	PPI
MS.gene04452	MS.gene061867	PPI
MS.gene09871	MS.gene061867	PPI
MS.gene061867	MS.gene60093	PPI
MS.gene04576	MS.gene061867	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061867.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061867.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene061867.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061867.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061867.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAGATCTTCT+TGG	0.324000	7.4:+10741738	MS.gene061867:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.412951	7.4:+10741592	MS.gene061867:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.4:-10741651	None:intergenic
AAAATGTCTGGCCGCGGAAA+AGG	0.446222	7.4:+10741568	None:intergenic
TGTTTATGCTTTGAAGAGAC+AGG	0.447929	7.4:+10741831	MS.gene061867:CDS
GGAGGAAAGGGATTGGGAAA+GGG	0.462159	7.4:+10741589	MS.gene061867:CDS
GGAAGGACTCTCTACGGTTT+CGG	0.462635	7.4:+10741853	MS.gene061867:CDS
AGGAGGAAAGGGATTGGGAA+AGG	0.466050	7.4:+10741588	MS.gene061867:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	7.4:+10741583	MS.gene061867:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.482086	7.4:+10741582	MS.gene061867:CDS
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.4:+10741668	MS.gene061867:CDS
GGCCGCGGAAAAGGAGGAAA+GGG	0.550993	7.4:+10741577	MS.gene061867:CDS
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.4:-10741667	None:intergenic
AGGAGCAAAGAGGCACAGAA+AGG	0.564264	7.4:+10741612	MS.gene061867:CDS
TTACTTACACTGAGCACGCT+AGG	0.572434	7.4:+10741782	MS.gene061867:CDS
ATGTCTGGCCGCGGAAAAGG+AGG	0.578066	7.4:+10741571	MS.gene061867:CDS
GAGGAAGACTGTTACCGCCA+TGG	0.588179	7.4:+10741804	MS.gene061867:CDS
TGGCCGCGGAAAAGGAGGAA+AGG	0.594714	7.4:+10741576	MS.gene061867:CDS
AGACAGGGAAGGACTCTCTA+CGG	0.604561	7.4:+10741847	MS.gene061867:CDS
AGGACTCTCTACGGTTTCGG+TGG	0.607233	7.4:+10741856	MS.gene061867:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	7.4:-10741821	None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.4:+10741676	MS.gene061867:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.618996	7.4:+10741832	MS.gene061867:CDS
ATGCTTTGAAGAGACAGGGA+AGG	0.619457	7.4:+10741836	MS.gene061867:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.4:+10741673	MS.gene061867:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.4:+10741634	MS.gene061867:CDS
AGCGTGCTCAGTGTAAGTAA+CGG	0.650649	7.4:-10741779	None:intergenic
CTTACACTGAGCACGCTAGG+AGG	0.678435	7.4:+10741785	MS.gene061867:CDS
TGGGAAAGGGAGGAGCAAAG+AGG	0.685544	7.4:+10741602	MS.gene061867:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7.4:+10741694	MS.gene061867:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7.4:+10741718	MS.gene061867:CDS
AGCATAAACAACATCCATGG+CGG	0.725411	7.4:-10741818	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	-	chr7.4:10741824-10741843	None:intergenic	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	+	chr7.4:10741832-10741851	MS.gene061867:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	+	chr7.4:10741831-10741850	MS.gene061867:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	-	chr7.4:10741821-10741840	None:intergenic	40.0%
	AGCGTGCTCAGTGTAAGTAA+CGG	-	chr7.4:10741782-10741801	None:intergenic	45.0%
	CGGTGTTCTCAAGATCTTCT+TGG	+	chr7.4:10741738-10741757	MS.gene061867:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.4:10741718-10741737	MS.gene061867:CDS	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	+	chr7.4:10741583-10741602	MS.gene061867:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.4:10741634-10741653	MS.gene061867:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.4:10741782-10741801	MS.gene061867:CDS	45.0%
!!	ATGCTTTGAAGAGACAGGGA+AGG	+	chr7.4:10741836-10741855	MS.gene061867:CDS	45.0%
	AGACAGGGAAGGACTCTCTA+CGG	+	chr7.4:10741847-10741866	MS.gene061867:CDS	50.0%
	AGGAGGAAAGGGATTGGGAA+AGG	+	chr7.4:10741588-10741607	MS.gene061867:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	+	chr7.4:10741582-10741601	MS.gene061867:CDS	50.0%
	GGAAGGACTCTCTACGGTTT+CGG	+	chr7.4:10741853-10741872	MS.gene061867:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+GGG	+	chr7.4:10741589-10741608	MS.gene061867:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr7.4:10741694-10741713	MS.gene061867:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	+	chr7.4:10741612-10741631	MS.gene061867:CDS	50.0%
!	ATCCCTTTCCTCCTTTTCCG+CGG	-	chr7.4:10741582-10741601	None:intergenic	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.4:10741654-10741673	None:intergenic	50.0%
	AGGACTCTCTACGGTTTCGG+TGG	+	chr7.4:10741856-10741875	MS.gene061867:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.4:10741673-10741692	MS.gene061867:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.4:10741668-10741687	MS.gene061867:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	+	chr7.4:10741785-10741804	MS.gene061867:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.4:10741670-10741689	None:intergenic	55.0%
	GAGGAAGACTGTTACCGCCA+TGG	+	chr7.4:10741804-10741823	MS.gene061867:CDS	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	+	chr7.4:10741592-10741611	MS.gene061867:CDS	55.0%
	TGGGAAAGGGAGGAGCAAAG+AGG	+	chr7.4:10741602-10741621	MS.gene061867:CDS	55.0%
	ATGTCTGGCCGCGGAAAAGG+AGG	+	chr7.4:10741571-10741590	MS.gene061867:CDS	60.0%
	GGCCGCGGAAAAGGAGGAAA+GGG	+	chr7.4:10741577-10741596	MS.gene061867:CDS	60.0%
	TGGCCGCGGAAAAGGAGGAA+AGG	+	chr7.4:10741576-10741595	MS.gene061867:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.4:10741676-10741695	MS.gene061867:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10741571	10741882	10741571	ID=MS.gene061867
chr7.4	mRNA	10741571	10741882	10741571	ID=MS.gene061867.t1;Parent=MS.gene061867
chr7.4	exon	10741571	10741882	10741571	ID=MS.gene061867.t1.exon1;Parent=MS.gene061867.t1
chr7.4	CDS	10741571	10741882	10741571	ID=cds.MS.gene061867.t1;Parent=MS.gene061867.t1

Gene Sequence	>MS.gene061867 ATGTCTGGCCGCGGAAAAGGAGGAAAGGGATTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAGATCTTCTTGGAGAACGTGATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGAAGGACTCTCTACGGTTTCGGTGGTTAA
Protein sequence	>MS.gene061867.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG