AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88680.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene88680.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene88680.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88680.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene88680.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 32 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGGGAAGGACTCTTTA+TGG 0.247348 8.4:-6202460 MS.gene88680:CDS
GGAAGGACTCTTTATGGATT+TGG 0.297351 8.4:-6202454 MS.gene88680:CDS
TCCTTTGCCTCTTCCTGACA+TGG 0.418595 8.4:+6202738 None:intergenic
TCTTTATGGATTTGGCGGTT+AGG 0.439485 8.4:-6202446 None:intergenic
GGCAAAGGAGGAAAGGGTCT+TGG 0.446329 8.4:-6202724 MS.gene88680:CDS
CAAGAATTCACAGCCATGTC+AGG 0.481900 8.4:-6202751 None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG 0.488613 8.4:-6202476 MS.gene88680:CDS
GGTTCTTCGCGATAACATCC+AGG 0.500175 8.4:-6202674 MS.gene88680:CDS
AGGACTCTTTATGGATTTGG+CGG 0.509079 8.4:-6202451 MS.gene88680:CDS
GGAGGAAAGGGTCTTGGAAA+GGG 0.511441 8.4:-6202718 MS.gene88680:CDS
TCGCAGGCTTCGTGATTCCC+TGG 0.511861 8.4:+6202656 None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 8.4:-6202731 MS.gene88680:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 8.4:-6202730 MS.gene88680:CDS
CCATCGCAGTAACAGTCTTA+CGG 0.545551 8.4:+6202503 None:intergenic
GCCATGTCAGGAAGAGGCAA+AGG 0.547501 8.4:-6202739 MS.gene88680:CDS
AGGAGGAAAGGGTCTTGGAA+AGG 0.553664 8.4:-6202719 MS.gene88680:CDS
CCGTAAGACTGTTACTGCGA+TGG 0.561095 8.4:-6202503 MS.gene88680:CDS
CTTGCGAGACGACGAATCGC+AGG 0.571080 8.4:+6202640 None:intergenic
TTCACAGCCATGTCAGGAAG+AGG 0.573281 8.4:-6202745 None:intergenic
AGGAGCAAAACGACACCGTA+AGG 0.575994 8.4:-6202695 MS.gene88680:CDS
TGTTATCGCGAAGAACCTTA+CGG 0.576355 8.4:+6202680 None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG 0.581057 8.4:-6202736 MS.gene88680:CDS
ACGCTCTCAAGAGGCAGGGA+AGG 0.583359 8.4:-6202471 MS.gene88680:CDS
ACGGCGAGCATGCTCTGTGT+AGG 0.590446 8.4:+6202522 None:intergenic
GGAAAGGGTCTTGGAAAGGG+AGG 0.621886 8.4:-6202715 MS.gene88680:CDS
ATTCGTCGTCTCGCAAGACG+TGG 0.624267 8.4:-6202634 MS.gene88680:CDS
TTAATCTATGAAGAAACTCG+TGG 0.634992 8.4:-6202589 MS.gene88680:CDS
ATGTCGTTTACGCTCTCAAG+AGG 0.636714 8.4:-6202480 MS.gene88680:CDS
CGTCGTCTCGCAAGACGTGG+TGG 0.642630 8.4:-6202631 MS.gene88680:CDS
GTTCTTCGCGATAACATCCA+GGG 0.657095 8.4:-6202673 MS.gene88680:CDS
GTTTACGCTCTCAAGAGGCA+GGG 0.663401 8.4:-6202475 MS.gene88680:CDS
GGTGGTGTGAAGCGTATCAG+TGG 0.669421 8.4:-6202613 MS.gene88680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAATCTATGAAGAAACTCG+TGG - chr8.4:6202594-6202613 MS.gene88680:CDS 30.0%
AGGACTCTTTATGGATTTGG+CGG - chr8.4:6202732-6202751 MS.gene88680:CDS 40.0%
GGAAGGACTCTTTATGGATT+TGG - chr8.4:6202729-6202748 MS.gene88680:CDS 40.0%
TGTTATCGCGAAGAACCTTA+CGG + chr8.4:6202506-6202525 None:intergenic 40.0%
CCATCGCAGTAACAGTCTTA+CGG + chr8.4:6202683-6202702 None:intergenic 45.0%
GTTCTTCGCGATAACATCCA+GGG - chr8.4:6202510-6202529 MS.gene88680:CDS 45.0%
! ATGTCGTTTACGCTCTCAAG+AGG - chr8.4:6202703-6202722 MS.gene88680:CDS 45.0%
AGGAAGAGGCAAAGGAGGAA+AGG - chr8.4:6202452-6202471 MS.gene88680:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG - chr8.4:6202488-6202507 MS.gene88680:CDS 50.0%
AGGAGGAAAGGGTCTTGGAA+AGG - chr8.4:6202464-6202483 MS.gene88680:CDS 50.0%
AGGCAGGGAAGGACTCTTTA+TGG - chr8.4:6202723-6202742 MS.gene88680:CDS 50.0%
ATGTCAGGAAGAGGCAAAGG+AGG - chr8.4:6202447-6202466 MS.gene88680:CDS 50.0%
CCGTAAGACTGTTACTGCGA+TGG - chr8.4:6202680-6202699 MS.gene88680:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG - chr8.4:6202453-6202472 MS.gene88680:CDS 50.0%
GGAGGAAAGGGTCTTGGAAA+GGG - chr8.4:6202465-6202484 MS.gene88680:CDS 50.0%
GGTTCTTCGCGATAACATCC+AGG - chr8.4:6202509-6202528 MS.gene88680:CDS 50.0%
GTTTACGCTCTCAAGAGGCA+GGG - chr8.4:6202708-6202727 MS.gene88680:CDS 50.0%
ATTCGTCGTCTCGCAAGACG+TGG - chr8.4:6202549-6202568 MS.gene88680:CDS 55.0%
CGTTTACGCTCTCAAGAGGC+AGG - chr8.4:6202707-6202726 MS.gene88680:CDS 55.0%
GGAAAGGGTCTTGGAAAGGG+AGG - chr8.4:6202468-6202487 MS.gene88680:CDS 55.0%
GGCAAAGGAGGAAAGGGTCT+TGG - chr8.4:6202459-6202478 MS.gene88680:CDS 55.0%
GGTGGTGTGAAGCGTATCAG+TGG - chr8.4:6202570-6202589 MS.gene88680:CDS 55.0%
ACGCTCTCAAGAGGCAGGGA+AGG - chr8.4:6202712-6202731 MS.gene88680:CDS 60.0%
ACGGCGAGCATGCTCTGTGT+AGG + chr8.4:6202664-6202683 None:intergenic 60.0%
CTTGCGAGACGACGAATCGC+AGG + chr8.4:6202546-6202565 None:intergenic 60.0%
TCGCAGGCTTCGTGATTCCC+TGG + chr8.4:6202530-6202549 None:intergenic 60.0%
CGTCGTCTCGCAAGACGTGG+TGG - chr8.4:6202552-6202571 MS.gene88680:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 6202447 6202758 6202447 ID=MS.gene88680
chr8.4 mRNA 6202447 6202758 6202447 ID=MS.gene88680.t1;Parent=MS.gene88680
chr8.4 exon 6202447 6202758 6202447 ID=MS.gene88680.t1.exon1;Parent=MS.gene88680.t1
chr8.4 CDS 6202447 6202758 6202447 ID=cds.MS.gene88680.t1;Parent=MS.gene88680.t1
Gene Sequence

>MS.gene88680

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTTGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATCACGAAGCCTGCGATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG

Protein sequence

>MS.gene88680.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG