AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061866


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061866.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061866.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene061866.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061866.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061866.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGTTCTCAAAATCTTCT+TGG 0.286841 7.4:+10747714 MS.gene061866:CDS
GGAAGGACCCTTTACGGTTT+CGG 0.307868 7.4:+10747829 MS.gene061866:CDS
TGGAAAAGGTGGAAAAGGTT+TGG 0.419608 7.4:+10747558 MS.gene061866:CDS
GGAAAAGGTGGAAAAGGTTT+GGG 0.432725 7.4:+10747559 MS.gene061866:CDS
AGGCAGGGAAGGACCCTTTA+CGG 0.435749 7.4:+10747823 MS.gene061866:CDS
TCGCAGGCTTTGTTATGCCT+TGG 0.436279 7.4:-10747627 None:intergenic
GGTGGAAAAGGTTTGGGAAA+GGG 0.442921 7.4:+10747565 MS.gene061866:CDS
TCAACCACCGAAACCGTAAA+GGG 0.488164 7.4:-10747836 None:intergenic
CTGCGATTCGTCGTCTTGCA+AGG 0.502831 7.4:+10747644 MS.gene061866:CDS
TCAGTGCAAGCGAAAATGTC+AGG 0.509801 7.4:+10747532 None:intergenic
TGTCTATGCTCTCAAGAGGC+AGG 0.510611 7.4:+10747807 MS.gene061866:CDS
AAAATGTCAGGTCGTGGAAA+AGG 0.512942 7.4:+10747544 None:intergenic
GGAAAAGGTTTGGGAAAGGG+AGG 0.514769 7.4:+10747568 MS.gene061866:CDS
ATCAACCACCGAAACCGTAA+AGG 0.525393 7.4:-10747837 None:intergenic
GGTCGTGGAAAAGGTGGAAA+AGG 0.536368 7.4:+10747553 MS.gene061866:CDS
TTACTTACACTGAGCACGCT+AGG 0.551062 7.4:+10747758 MS.gene061866:CDS
CAAGCGAAAATGTCAGGTCG+TGG 0.551935 7.4:+10747538 None:intergenic
CTTGCAAGACGACGAATCGC+AGG 0.552192 7.4:-10747643 None:intergenic
ATGCTCTCAAGAGGCAGGGA+AGG 0.564763 7.4:+10747812 MS.gene061866:CDS
AGGTGGAAAAGGTTTGGGAA+AGG 0.583555 7.4:+10747564 MS.gene061866:CDS
GTCTATGCTCTCAAGAGGCA+GGG 0.585715 7.4:+10747808 MS.gene061866:CDS
GAGAAAGACTGTTACCGCCA+TGG 0.588179 7.4:+10747780 MS.gene061866:CDS
ATGTCAGGTCGTGGAAAAGG+TGG 0.588325 7.4:+10747547 MS.gene061866:CDS
AGGAGCAAAGAGACACAGAA+AGG 0.597296 7.4:+10747588 MS.gene061866:CDS
AGGACCCTTTACGGTTTCGG+TGG 0.602372 7.4:+10747832 MS.gene061866:CDS
CGTCGTCTTGCAAGGCGTGG+TGG 0.615268 7.4:+10747652 MS.gene061866:CDS
ATTCGTCGTCTTGCAAGGCG+TGG 0.619919 7.4:+10747649 MS.gene061866:CDS
GTTCTTCGCGATAACATCCA+AGG 0.633409 7.4:+10747610 MS.gene061866:CDS
GAGAGCATAGACAACATCCA+TGG 0.645428 7.4:-10747797 None:intergenic
ATGTTGTCTATGCTCTCAAG+AGG 0.677658 7.4:+10747803 MS.gene061866:CDS
GGTGGTGTGAAGAGAATCAG+CGG 0.689951 7.4:+10747670 MS.gene061866:CDS
CTCATCTATGAAGAGACTCG+CGG 0.713431 7.4:+10747694 MS.gene061866:CDS
AGCATAGACAACATCCATGG+CGG 0.735882 7.4:-10747794 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGTTGTCTATGCTCTCAAG+AGG + chr7.4:10747803-10747822 MS.gene061866:CDS 40.0%
CGGTGTTCTCAAAATCTTCT+TGG + chr7.4:10747714-10747733 MS.gene061866:CDS 40.0%
GGAAAAGGTGGAAAAGGTTT+GGG + chr7.4:10747559-10747578 MS.gene061866:CDS 40.0%
TGGAAAAGGTGGAAAAGGTT+TGG + chr7.4:10747558-10747577 MS.gene061866:CDS 40.0%
AGCATAGACAACATCCATGG+CGG - chr7.4:10747797-10747816 None:intergenic 45.0%
AGGAGCAAAGAGACACAGAA+AGG + chr7.4:10747588-10747607 MS.gene061866:CDS 45.0%
AGGTGGAAAAGGTTTGGGAA+AGG + chr7.4:10747564-10747583 MS.gene061866:CDS 45.0%
CTCATCTATGAAGAGACTCG+CGG + chr7.4:10747694-10747713 MS.gene061866:CDS 45.0%
GGTGGAAAAGGTTTGGGAAA+GGG + chr7.4:10747565-10747584 MS.gene061866:CDS 45.0%
GTTCTTCGCGATAACATCCA+AGG + chr7.4:10747610-10747629 MS.gene061866:CDS 45.0%
TCAACCACCGAAACCGTAAA+GGG - chr7.4:10747839-10747858 None:intergenic 45.0%
TTACTTACACTGAGCACGCT+AGG + chr7.4:10747758-10747777 MS.gene061866:CDS 45.0%
! GAGAGCATAGACAACATCCA+TGG - chr7.4:10747800-10747819 None:intergenic 45.0%
ATGTCAGGTCGTGGAAAAGG+TGG + chr7.4:10747547-10747566 MS.gene061866:CDS 50.0%
GAGAAAGACTGTTACCGCCA+TGG + chr7.4:10747780-10747799 MS.gene061866:CDS 50.0%
GGAAAAGGTTTGGGAAAGGG+AGG + chr7.4:10747568-10747587 MS.gene061866:CDS 50.0%
GGAAGGACCCTTTACGGTTT+CGG + chr7.4:10747829-10747848 MS.gene061866:CDS 50.0%
GGTCGTGGAAAAGGTGGAAA+AGG + chr7.4:10747553-10747572 MS.gene061866:CDS 50.0%
GGTGGTGTGAAGAGAATCAG+CGG + chr7.4:10747670-10747689 MS.gene061866:CDS 50.0%
GTCTATGCTCTCAAGAGGCA+GGG + chr7.4:10747808-10747827 MS.gene061866:CDS 50.0%
TGTCTATGCTCTCAAGAGGC+AGG + chr7.4:10747807-10747826 MS.gene061866:CDS 50.0%
!! TCGCAGGCTTTGTTATGCCT+TGG - chr7.4:10747630-10747649 None:intergenic 50.0%
AGGACCCTTTACGGTTTCGG+TGG + chr7.4:10747832-10747851 MS.gene061866:CDS 55.0%
AGGCAGGGAAGGACCCTTTA+CGG + chr7.4:10747823-10747842 MS.gene061866:CDS 55.0%
ATGCTCTCAAGAGGCAGGGA+AGG + chr7.4:10747812-10747831 MS.gene061866:CDS 55.0%
ATTCGTCGTCTTGCAAGGCG+TGG + chr7.4:10747649-10747668 MS.gene061866:CDS 55.0%
CTGCGATTCGTCGTCTTGCA+AGG + chr7.4:10747644-10747663 MS.gene061866:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG - chr7.4:10747646-10747665 None:intergenic 55.0%
CGTCGTCTTGCAAGGCGTGG+TGG + chr7.4:10747652-10747671 MS.gene061866:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 10747547 10747858 10747547 ID=MS.gene061866
chr7.4 mRNA 10747547 10747858 10747547 ID=MS.gene061866.t1;Parent=MS.gene061866
chr7.4 exon 10747547 10747858 10747547 ID=MS.gene061866.t1.exon1;Parent=MS.gene061866.t1
chr7.4 CDS 10747547 10747858 10747547 ID=cds.MS.gene061866.t1;Parent=MS.gene061866.t1
Gene Sequence

>MS.gene061866

ATGTCAGGTCGTGGAAAAGGTGGAAAAGGTTTGGGAAAGGGAGGAGCAAAGAGACACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGCGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAAATCTTCTTGGAAAACGTGATTCGCGATGCTGTTACTTACACTGAGCACGCTAGGAGAAAGACTGTTACCGCCATGGATGTTGTCTATGCTCTCAAGAGGCAGGGAAGGACCCTTTACGGTTTCGGTGGTTGA

Protein sequence

>MS.gene061866.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG