AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061874


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061874.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene061874.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene061874.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061874.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene061874.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGTTCTCAAAATCTTCT+TGG 0.286841 7.4:+10655532 MS.gene061874:CDS
GGAAGGACCCTTTACGGTTT+CGG 0.307868 7.4:+10655647 MS.gene061874:CDS
TGGAAAAGGTGGAAAAGGTT+TGG 0.419608 7.4:+10655376 MS.gene061874:CDS
GGAAAAGGTGGAAAAGGTTT+GGG 0.432725 7.4:+10655377 MS.gene061874:CDS
AGGCAGGGAAGGACCCTTTA+CGG 0.435749 7.4:+10655641 MS.gene061874:CDS
TCGCAGGCTTTGTTATGCCT+TGG 0.436279 7.4:-10655445 None:intergenic
GGTGGAAAAGGTTTGGGAAA+GGG 0.442921 7.4:+10655383 MS.gene061874:CDS
TCAACCACCGAAACCGTAAA+GGG 0.488164 7.4:-10655654 None:intergenic
CTGCGATTCGTCGTCTTGCA+AGG 0.502831 7.4:+10655462 MS.gene061874:CDS
TCAGTGCAAGCGAAAATGTC+AGG 0.509801 7.4:+10655350 None:intergenic
TGTCTATGCTCTCAAGAGGC+AGG 0.510611 7.4:+10655625 MS.gene061874:CDS
AAAATGTCAGGTCGTGGAAA+AGG 0.512942 7.4:+10655362 None:intergenic
GGAAAAGGTTTGGGAAAGGG+AGG 0.514769 7.4:+10655386 MS.gene061874:CDS
ATCAACCACCGAAACCGTAA+AGG 0.525393 7.4:-10655655 None:intergenic
GGTCGTGGAAAAGGTGGAAA+AGG 0.536368 7.4:+10655371 MS.gene061874:CDS
TTACTTACACTGAGCACGCT+AGG 0.551062 7.4:+10655576 MS.gene061874:CDS
CAAGCGAAAATGTCAGGTCG+TGG 0.551935 7.4:+10655356 None:intergenic
CTTGCAAGACGACGAATCGC+AGG 0.552192 7.4:-10655461 None:intergenic
ATGCTCTCAAGAGGCAGGGA+AGG 0.564763 7.4:+10655630 MS.gene061874:CDS
AGGTGGAAAAGGTTTGGGAA+AGG 0.583555 7.4:+10655382 MS.gene061874:CDS
GTCTATGCTCTCAAGAGGCA+GGG 0.585715 7.4:+10655626 MS.gene061874:CDS
GAGAAAGACTGTTACCGCCA+TGG 0.588179 7.4:+10655598 MS.gene061874:CDS
ATGTCAGGTCGTGGAAAAGG+TGG 0.588325 7.4:+10655365 MS.gene061874:CDS
AGGAGCAAAGAGACACAGAA+AGG 0.597296 7.4:+10655406 MS.gene061874:CDS
AGGACCCTTTACGGTTTCGG+TGG 0.602372 7.4:+10655650 MS.gene061874:CDS
CGTCGTCTTGCAAGGCGTGG+TGG 0.615268 7.4:+10655470 MS.gene061874:CDS
ATTCGTCGTCTTGCAAGGCG+TGG 0.619919 7.4:+10655467 MS.gene061874:CDS
GTTCTTCGCGATAACATCCA+AGG 0.633409 7.4:+10655428 MS.gene061874:CDS
GAGAGCATAGACAACATCCA+TGG 0.645428 7.4:-10655615 None:intergenic
ATGTTGTCTATGCTCTCAAG+AGG 0.677658 7.4:+10655621 MS.gene061874:CDS
GGTGGTGTGAAGAGAATCAG+CGG 0.689951 7.4:+10655488 MS.gene061874:CDS
CTCATCTATGAAGAGACTCG+CGG 0.713431 7.4:+10655512 MS.gene061874:CDS
AGCATAGACAACATCCATGG+CGG 0.735882 7.4:-10655612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGTTGTCTATGCTCTCAAG+AGG + chr7.4:10655621-10655640 MS.gene061874:CDS 40.0%
CGGTGTTCTCAAAATCTTCT+TGG + chr7.4:10655532-10655551 MS.gene061874:CDS 40.0%
GGAAAAGGTGGAAAAGGTTT+GGG + chr7.4:10655377-10655396 MS.gene061874:CDS 40.0%
TGGAAAAGGTGGAAAAGGTT+TGG + chr7.4:10655376-10655395 MS.gene061874:CDS 40.0%
AGCATAGACAACATCCATGG+CGG - chr7.4:10655615-10655634 None:intergenic 45.0%
AGGAGCAAAGAGACACAGAA+AGG + chr7.4:10655406-10655425 MS.gene061874:CDS 45.0%
AGGTGGAAAAGGTTTGGGAA+AGG + chr7.4:10655382-10655401 MS.gene061874:CDS 45.0%
CTCATCTATGAAGAGACTCG+CGG + chr7.4:10655512-10655531 MS.gene061874:CDS 45.0%
GGTGGAAAAGGTTTGGGAAA+GGG + chr7.4:10655383-10655402 MS.gene061874:CDS 45.0%
GTTCTTCGCGATAACATCCA+AGG + chr7.4:10655428-10655447 MS.gene061874:CDS 45.0%
TCAACCACCGAAACCGTAAA+GGG - chr7.4:10655657-10655676 None:intergenic 45.0%
TTACTTACACTGAGCACGCT+AGG + chr7.4:10655576-10655595 MS.gene061874:CDS 45.0%
! GAGAGCATAGACAACATCCA+TGG - chr7.4:10655618-10655637 None:intergenic 45.0%
ATGTCAGGTCGTGGAAAAGG+TGG + chr7.4:10655365-10655384 MS.gene061874:CDS 50.0%
GAGAAAGACTGTTACCGCCA+TGG + chr7.4:10655598-10655617 MS.gene061874:CDS 50.0%
GGAAAAGGTTTGGGAAAGGG+AGG + chr7.4:10655386-10655405 MS.gene061874:CDS 50.0%
GGAAGGACCCTTTACGGTTT+CGG + chr7.4:10655647-10655666 MS.gene061874:CDS 50.0%
GGTCGTGGAAAAGGTGGAAA+AGG + chr7.4:10655371-10655390 MS.gene061874:CDS 50.0%
GGTGGTGTGAAGAGAATCAG+CGG + chr7.4:10655488-10655507 MS.gene061874:CDS 50.0%
GTCTATGCTCTCAAGAGGCA+GGG + chr7.4:10655626-10655645 MS.gene061874:CDS 50.0%
TGTCTATGCTCTCAAGAGGC+AGG + chr7.4:10655625-10655644 MS.gene061874:CDS 50.0%
!! TCGCAGGCTTTGTTATGCCT+TGG - chr7.4:10655448-10655467 None:intergenic 50.0%
AGGACCCTTTACGGTTTCGG+TGG + chr7.4:10655650-10655669 MS.gene061874:CDS 55.0%
AGGCAGGGAAGGACCCTTTA+CGG + chr7.4:10655641-10655660 MS.gene061874:CDS 55.0%
ATGCTCTCAAGAGGCAGGGA+AGG + chr7.4:10655630-10655649 MS.gene061874:CDS 55.0%
ATTCGTCGTCTTGCAAGGCG+TGG + chr7.4:10655467-10655486 MS.gene061874:CDS 55.0%
CTGCGATTCGTCGTCTTGCA+AGG + chr7.4:10655462-10655481 MS.gene061874:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG - chr7.4:10655464-10655483 None:intergenic 55.0%
CGTCGTCTTGCAAGGCGTGG+TGG + chr7.4:10655470-10655489 MS.gene061874:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 10655365 10655676 10655365 ID=MS.gene061874
chr7.4 mRNA 10655365 10655676 10655365 ID=MS.gene061874.t1;Parent=MS.gene061874
chr7.4 exon 10655365 10655676 10655365 ID=MS.gene061874.t1.exon1;Parent=MS.gene061874.t1
chr7.4 CDS 10655365 10655676 10655365 ID=cds.MS.gene061874.t1;Parent=MS.gene061874.t1
Gene Sequence

>MS.gene061874

ATGTCAGGTCGTGGAAAAGGTGGAAAAGGTTTGGGAAAGGGAGGAGCAAAGAGACACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGCGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAAATCTTCTTGGAAAACGTGATTCGCGATGCTGTTACTTACACTGAGCACGCTAGGAGAAAGACTGTTACCGCCATGGATGTTGTCTATGCTCTCAAGAGGCAGGGAAGGACCCTTTACGGTTTCGGTGGTTGA

Protein sequence

>MS.gene061874.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG