AlfalfaGEDB | MS.gene061869

M. sativa cultivar XinJiangDaYe / MS.gene061869

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061869.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061869.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061869.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene061869	0.852756	3.75E-61	-1.69E-46
MS.gene049159	MS.gene061869	0.839105	1.93E-57	-1.69E-46
MS.gene049254	MS.gene061869	0.804856	1.77E-49	-1.69E-46
MS.gene049255	MS.gene061869	0.843308	1.52E-58	-1.69E-46
MS.gene049341	MS.gene061869	0.830322	3.13E-55	-1.69E-46
MS.gene049350	MS.gene061869	0.839933	1.18E-57	-1.69E-46
MS.gene049368	MS.gene061869	0.800598	1.34E-48	-1.69E-46
MS.gene049501	MS.gene061869	0.818775	1.64E-52	-1.69E-46
MS.gene049711	MS.gene061869	0.811144	8.11E-51	-1.69E-46
MS.gene049807	MS.gene061869	0.800189	1.62E-48	-1.69E-46
MS.gene049821	MS.gene061869	0.890507	9.25E-74	-1.69E-46
MS.gene049825	MS.gene061869	0.80965	1.70E-50	-1.69E-46
MS.gene049850	MS.gene061869	0.862499	4.85E-64	-1.69E-46
MS.gene049873	MS.gene061869	0.831851	1.32E-55	-1.69E-46
MS.gene050020	MS.gene061869	0.851201	1.04E-60	-1.69E-46
MS.gene05045	MS.gene061869	0.828907	6.92E-55	-1.69E-46
MS.gene050689	MS.gene061869	0.864139	1.51E-64	-1.69E-46
MS.gene050807	MS.gene061869	0.848357	6.45E-60	-1.69E-46
MS.gene050808	MS.gene061869	0.800371	1.49E-48	-1.69E-46
MS.gene050815	MS.gene061869	0.811216	7.82E-51	-1.69E-46
MS.gene050838	MS.gene061869	0.814107	1.82E-51	-1.69E-46
MS.gene050901	MS.gene061869	0.894137	3.27E-75	-1.69E-46
MS.gene051018	MS.gene061869	0.815927	7.19E-52	-1.69E-46
MS.gene051180	MS.gene061869	0.808013	3.81E-50	-1.69E-46
MS.gene051234	MS.gene061869	0.802857	4.60E-49	-1.69E-46
MS.gene051243	MS.gene061869	0.806258	8.98E-50	-1.69E-46
MS.gene051247	MS.gene061869	0.850718	1.42E-60	-1.69E-46
MS.gene051328	MS.gene061869	0.886457	3.36E-72	-1.69E-46
MS.gene05134	MS.gene061869	0.823433	1.39E-53	-1.69E-46
MS.gene05157	MS.gene061869	0.845975	2.90E-59	-1.69E-46
MS.gene051664	MS.gene061869	0.838888	2.20E-57	-1.69E-46
MS.gene051700	MS.gene061869	0.849385	3.35E-60	-1.69E-46
MS.gene051719	MS.gene061869	0.876948	9.30E-69	-1.69E-46
MS.gene051720	MS.gene061869	0.87966	1.04E-69	-1.69E-46
MS.gene051744	MS.gene061869	0.847414	1.17E-59	-1.69E-46
MS.gene05188	MS.gene061869	0.815306	9.89E-52	-1.69E-46
MS.gene051957	MS.gene061869	0.805291	1.43E-49	-1.69E-46
MS.gene051975	MS.gene061869	0.819427	1.17E-52	-1.69E-46
MS.gene052012	MS.gene061869	0.803473	3.43E-49	-1.69E-46
MS.gene052020	MS.gene061869	0.815395	9.45E-52	-1.69E-46
MS.gene052133	MS.gene061869	0.856352	3.41E-62	-1.69E-46
MS.gene052516	MS.gene061869	0.86175	8.24E-64	-1.69E-46
MS.gene052519	MS.gene061869	0.855758	5.09E-62	-1.69E-46
MS.gene052582	MS.gene061869	0.804002	2.66E-49	-1.69E-46
MS.gene052584	MS.gene061869	0.813189	2.90E-51	-1.69E-46
MS.gene052611	MS.gene061869	0.836052	1.17E-56	-1.69E-46
MS.gene052773	MS.gene061869	0.834325	3.20E-56	-1.69E-46
MS.gene052863	MS.gene061869	0.83672	7.93E-57	-1.69E-46
MS.gene052879	MS.gene061869	0.822688	2.07E-53	-1.69E-46
MS.gene052880	MS.gene061869	0.813014	3.17E-51	-1.69E-46
MS.gene052896	MS.gene061869	0.800655	1.30E-48	-1.69E-46
MS.gene052947	MS.gene061869	0.842476	2.53E-58	-1.69E-46
MS.gene053008	MS.gene061869	0.838288	3.14E-57	-1.69E-46
MS.gene053122	MS.gene061869	0.848797	4.87E-60	-1.69E-46
MS.gene053144	MS.gene061869	0.834066	3.72E-56	-1.69E-46
MS.gene053226	MS.gene061869	0.87231	3.51E-67	-1.69E-46
MS.gene053542	MS.gene061869	0.80583	1.10E-49	-1.69E-46
MS.gene053563	MS.gene061869	0.833533	5.05E-56	-1.69E-46
MS.gene053581	MS.gene061869	0.864746	9.74E-65	-1.69E-46
MS.gene053599	MS.gene061869	0.831292	1.81E-55	-1.69E-46
MS.gene05364	MS.gene061869	0.851781	7.10E-61	-1.69E-46
MS.gene053883	MS.gene061869	0.815903	7.28E-52	-1.69E-46
MS.gene054142	MS.gene061869	0.814273	1.68E-51	-1.69E-46
MS.gene054236	MS.gene061869	0.820377	7.08E-53	-1.69E-46
MS.gene05423	MS.gene061869	0.813135	2.98E-51	-1.69E-46
MS.gene054472	MS.gene061869	0.810008	1.43E-50	-1.69E-46
MS.gene054584	MS.gene061869	0.824243	8.97E-54	-1.69E-46
MS.gene05458	MS.gene061869	0.825358	4.90E-54	-1.69E-46
MS.gene05462	MS.gene061869	0.800942	1.14E-48	-1.69E-46
MS.gene054651	MS.gene061869	0.85216	5.54E-61	-1.69E-46
MS.gene054775	MS.gene061869	0.832439	9.45E-56	-1.69E-46
MS.gene055144	MS.gene061869	0.810555	1.09E-50	-1.69E-46
MS.gene055162	MS.gene061869	0.813613	2.34E-51	-1.69E-46
MS.gene05517	MS.gene061869	0.822072	2.88E-53	-1.69E-46
MS.gene055465	MS.gene061869	0.869908	2.18E-66	-1.69E-46
MS.gene055466	MS.gene061869	0.891609	3.40E-74	-1.69E-46
MS.gene055468	MS.gene061869	0.87673	1.11E-68	-1.69E-46
MS.gene055469	MS.gene061869	0.815029	1.14E-51	-1.69E-46
MS.gene055512	MS.gene061869	0.839351	1.67E-57	-1.69E-46
MS.gene055513	MS.gene061869	0.845771	3.30E-59	-1.69E-46
MS.gene055537	MS.gene061869	0.841987	3.40E-58	-1.69E-46
MS.gene055580	MS.gene061869	-0.875252	3.57E-68	-1.69E-46
MS.gene055623	MS.gene061869	0.8024	5.71E-49	-1.69E-46
MS.gene055694	MS.gene061869	0.82004	8.46E-53	-1.69E-46
MS.gene055778	MS.gene061869	0.832256	1.05E-55	-1.69E-46
MS.gene055808	MS.gene061869	0.807098	5.97E-50	-1.69E-46
MS.gene055818	MS.gene061869	0.847908	8.58E-60	-1.69E-46
MS.gene055890	MS.gene061869	0.806108	9.65E-50	-1.69E-46
MS.gene055891	MS.gene061869	0.818473	1.92E-52	-1.69E-46
MS.gene055979	MS.gene061869	0.804281	2.33E-49	-1.69E-46
MS.gene056005	MS.gene061869	0.850025	2.22E-60	-1.69E-46
MS.gene056043	MS.gene061869	-0.857148	1.99E-62	-1.69E-46
MS.gene05608	MS.gene061869	0.842356	2.72E-58	-1.69E-46
MS.gene056215	MS.gene061869	0.901308	3.06E-78	-1.69E-46
MS.gene05622	MS.gene061869	0.80392	2.77E-49	-1.69E-46
MS.gene056308	MS.gene061869	0.855743	5.14E-62	-1.69E-46
MS.gene056366	MS.gene061869	0.843842	1.09E-58	-1.69E-46
MS.gene056553	MS.gene061869	0.803257	3.80E-49	-1.69E-46
MS.gene056595	MS.gene061869	0.858285	9.15E-63	-1.69E-46
MS.gene05690	MS.gene061869	0.800997	1.11E-48	-1.69E-46
MS.gene057034	MS.gene061869	0.825577	4.34E-54	-1.69E-46
MS.gene057060	MS.gene061869	0.808337	3.26E-50	-1.69E-46
MS.gene057208	MS.gene061869	0.80997	1.45E-50	-1.69E-46
MS.gene057294	MS.gene061869	0.810504	1.12E-50	-1.69E-46
MS.gene057444	MS.gene061869	0.822058	2.90E-53	-1.69E-46
MS.gene057693	MS.gene061869	0.847105	1.43E-59	-1.69E-46
MS.gene057694	MS.gene061869	0.836959	6.89E-57	-1.69E-46
MS.gene057705	MS.gene061869	0.853936	1.72E-61	-1.69E-46
MS.gene057915	MS.gene061869	0.826991	2.00E-54	-1.69E-46
MS.gene058010	MS.gene061869	0.818904	1.54E-52	-1.69E-46
MS.gene058037	MS.gene061869	0.81271	3.70E-51	-1.69E-46
MS.gene058339	MS.gene061869	0.821743	3.43E-53	-1.69E-46
MS.gene05847	MS.gene061869	0.827098	1.89E-54	-1.69E-46
MS.gene058625	MS.gene061869	0.847323	1.24E-59	-1.69E-46
MS.gene058783	MS.gene061869	0.81782	2.71E-52	-1.69E-46
MS.gene058852	MS.gene061869	0.812158	4.88E-51	-1.69E-46
MS.gene058906	MS.gene061869	0.808474	3.04E-50	-1.69E-46
MS.gene05894	MS.gene061869	0.837288	5.68E-57	-1.69E-46
MS.gene058979	MS.gene061869	0.804456	2.14E-49	-1.69E-46
MS.gene059015	MS.gene061869	0.807885	4.06E-50	-1.69E-46
MS.gene059151	MS.gene061869	0.81687	4.43E-52	-1.69E-46
MS.gene05954	MS.gene061869	0.813838	2.09E-51	-1.69E-46
MS.gene059625	MS.gene061869	0.839345	1.67E-57	-1.69E-46
MS.gene05978	MS.gene061869	0.842282	2.84E-58	-1.69E-46
MS.gene059842	MS.gene061869	0.855488	6.10E-62	-1.69E-46
MS.gene059937	MS.gene061869	0.820278	7.47E-53	-1.69E-46
MS.gene06001	MS.gene061869	0.814952	1.19E-51	-1.69E-46
MS.gene060194	MS.gene061869	0.811227	7.78E-51	-1.69E-46
MS.gene060310	MS.gene061869	0.810972	8.84E-51	-1.69E-46
MS.gene060365	MS.gene061869	0.829576	4.76E-55	-1.69E-46
MS.gene060429	MS.gene061869	0.892877	1.06E-74	-1.69E-46
MS.gene060519	MS.gene061869	0.837361	5.44E-57	-1.69E-46
MS.gene060521	MS.gene061869	0.801203	1.01E-48	-1.69E-46
MS.gene060587	MS.gene061869	0.866485	2.75E-65	-1.69E-46
MS.gene060803	MS.gene061869	0.82664	2.43E-54	-1.69E-46
MS.gene060917	MS.gene061869	0.842332	2.76E-58	-1.69E-46
MS.gene061100	MS.gene061869	0.813588	2.37E-51	-1.69E-46
MS.gene061176	MS.gene061869	0.849218	3.72E-60	-1.69E-46
MS.gene061177	MS.gene061869	0.849742	2.66E-60	-1.69E-46
MS.gene061178	MS.gene061869	0.849118	3.97E-60	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene070241	MS.gene061869	PPI
MS.gene04434	MS.gene061869	PPI
MS.gene022946	MS.gene061869	PPI
MS.gene04064	MS.gene061869	PPI
MS.gene061876	MS.gene061869	PPI
MS.gene05619	MS.gene061869	PPI
MS.gene05608	MS.gene061869	PPI
MS.gene022932	MS.gene061869	PPI
MS.gene012391	MS.gene061869	PPI
MS.gene88681	MS.gene061869	PPI
MS.gene070244	MS.gene061869	PPI
MS.gene035897	MS.gene061869	PPI
MS.gene64920	MS.gene061869	PPI
MS.gene060463	MS.gene061869	PPI
MS.gene061869	MS.gene016624	PPI
MS.gene070907	MS.gene061869	PPI
MS.gene009027	MS.gene061869	PPI
MS.gene31196	MS.gene061869	PPI
MS.gene010219	MS.gene061869	PPI
MS.gene060465	MS.gene061869	PPI
MS.gene061867	MS.gene061869	PPI
MS.gene061869	MS.gene049705	PPI
MS.gene08121	MS.gene061869	PPI
MS.gene061870	MS.gene061869	PPI
MS.gene035718	MS.gene061869	PPI
MS.gene035896	MS.gene061869	PPI
MS.gene73136	MS.gene061869	PPI
MS.gene061869	MS.gene68545	PPI
MS.gene008276	MS.gene061869	PPI
MS.gene09873	MS.gene061869	PPI
MS.gene78578	MS.gene061869	PPI
MS.gene035721	MS.gene061869	PPI
MS.gene04052	MS.gene061869	PPI
MS.gene08122	MS.gene061869	PPI
MS.gene048705	MS.gene061869	PPI
MS.gene05623	MS.gene061869	PPI
MS.gene043967	MS.gene061869	PPI
MS.gene84536	MS.gene061869	PPI
MS.gene028629	MS.gene061869	PPI
MS.gene82546	MS.gene061869	PPI
MS.gene61017	MS.gene061869	PPI
MS.gene04433	MS.gene061869	PPI
MS.gene035787	MS.gene061869	PPI
MS.gene04067	MS.gene061869	PPI
MS.gene71215	MS.gene061869	PPI
MS.gene061866	MS.gene061869	PPI
MS.gene061869	MS.gene004124	PPI
MS.gene035719	MS.gene061869	PPI
MS.gene58854	MS.gene061869	PPI
MS.gene66225	MS.gene061869	PPI
MS.gene70955	MS.gene061869	PPI
MS.gene071136	MS.gene061869	PPI
MS.gene040383	MS.gene061869	PPI
MS.gene070242	MS.gene061869	PPI
MS.gene072493	MS.gene061869	PPI
MS.gene035720	MS.gene061869	PPI
MS.gene012390	MS.gene061869	PPI
MS.gene048746	MS.gene061869	PPI
MS.gene010220	MS.gene061869	PPI
MS.gene061869	MS.gene64650	PPI
MS.gene79195	MS.gene061869	PPI
MS.gene061869	MS.gene60093	PPI
MS.gene061869	MS.gene061874	PPI
MS.gene88680	MS.gene061869	PPI
MS.gene056215	MS.gene061869	PPI
MS.gene064557	MS.gene061869	PPI
MS.gene049821	MS.gene061869	PPI
MS.gene62520	MS.gene061869	PPI
MS.gene038469	MS.gene061869	PPI
MS.gene04452	MS.gene061869	PPI
MS.gene061869	MS.gene04972	PPI
MS.gene061869	MS.gene004121	PPI
MS.gene061869	MS.gene018578	PPI
MS.gene09871	MS.gene061869	PPI
MS.gene04576	MS.gene061869	PPI
MS.gene061869	MS.gene061867	PPI
MS.gene89487	MS.gene061869	PPI
MS.gene061869	MS.gene018580	PPI
MS.gene061869	MS.gene05619	PPI
MS.gene04392	MS.gene061869	PPI
MS.gene30920	MS.gene061869	PPI
MS.gene061869	MS.gene89488	PPI
MS.gene08053	MS.gene061869	PPI
MS.gene061872	MS.gene061869	PPI
MS.gene04068	MS.gene061869	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061869.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061869.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene061869.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061869.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061869.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAAATCTTCT+TGG	0.286841	7.4:+10734086	MS.gene061869:CDS
GGAAGGACCCTTTACGGTTT+CGG	0.307868	7.4:+10734201	MS.gene061869:CDS
TGGAAAAGGTGGAAAAGGTT+TGG	0.419608	7.4:+10733930	MS.gene061869:CDS
GGAAAAGGTGGAAAAGGTTT+GGG	0.432725	7.4:+10733931	MS.gene061869:CDS
AGGCAGGGAAGGACCCTTTA+CGG	0.435749	7.4:+10734195	MS.gene061869:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.4:-10733999	None:intergenic
GGTGGAAAAGGTTTGGGAAA+GGG	0.442921	7.4:+10733937	MS.gene061869:CDS
TCAACCACCGAAACCGTAAA+GGG	0.488164	7.4:-10734208	None:intergenic
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.4:+10734016	MS.gene061869:CDS
TCAGTGCAAGCGAAAATGTC+AGG	0.509801	7.4:+10733904	None:intergenic
TGTCTATGCTCTCAAGAGGC+AGG	0.510611	7.4:+10734179	MS.gene061869:CDS
AAAATGTCAGGTCGTGGAAA+AGG	0.512942	7.4:+10733916	None:intergenic
GGAAAAGGTTTGGGAAAGGG+AGG	0.514769	7.4:+10733940	MS.gene061869:CDS
ATCAACCACCGAAACCGTAA+AGG	0.525393	7.4:-10734209	None:intergenic
GGTCGTGGAAAAGGTGGAAA+AGG	0.536368	7.4:+10733925	MS.gene061869:CDS
TTACTTACACTGAGCACGCT+AGG	0.551062	7.4:+10734130	MS.gene061869:CDS
CAAGCGAAAATGTCAGGTCG+TGG	0.551935	7.4:+10733910	None:intergenic
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.4:-10734015	None:intergenic
ATGCTCTCAAGAGGCAGGGA+AGG	0.564763	7.4:+10734184	MS.gene061869:CDS
AGGTGGAAAAGGTTTGGGAA+AGG	0.583555	7.4:+10733936	MS.gene061869:CDS
GTCTATGCTCTCAAGAGGCA+GGG	0.585715	7.4:+10734180	MS.gene061869:CDS
GAGAAAGACTGTTACCGCCA+TGG	0.588179	7.4:+10734152	MS.gene061869:CDS
ATGTCAGGTCGTGGAAAAGG+TGG	0.588325	7.4:+10733919	MS.gene061869:CDS
AGGAGCAAAGAGACACAGAA+AGG	0.597296	7.4:+10733960	MS.gene061869:CDS
AGGACCCTTTACGGTTTCGG+TGG	0.602372	7.4:+10734204	MS.gene061869:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.4:+10734024	MS.gene061869:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.4:+10734021	MS.gene061869:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.4:+10733982	MS.gene061869:CDS
GAGAGCATAGACAACATCCA+TGG	0.645428	7.4:-10734169	None:intergenic
ATGTTGTCTATGCTCTCAAG+AGG	0.677658	7.4:+10734175	MS.gene061869:CDS
GGTGGTGTGAAGAGAATCAG+CGG	0.689951	7.4:+10734042	MS.gene061869:CDS
CTCATCTATGAAGAGACTCG+CGG	0.713431	7.4:+10734066	MS.gene061869:CDS
AGCATAGACAACATCCATGG+CGG	0.735882	7.4:-10734166	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGTTGTCTATGCTCTCAAG+AGG	+	chr7.4:10734175-10734194	MS.gene061869:CDS	40.0%
	CGGTGTTCTCAAAATCTTCT+TGG	+	chr7.4:10734086-10734105	MS.gene061869:CDS	40.0%
	GGAAAAGGTGGAAAAGGTTT+GGG	+	chr7.4:10733931-10733950	MS.gene061869:CDS	40.0%
	TGGAAAAGGTGGAAAAGGTT+TGG	+	chr7.4:10733930-10733949	MS.gene061869:CDS	40.0%
	AGCATAGACAACATCCATGG+CGG	-	chr7.4:10734169-10734188	None:intergenic	45.0%
	AGGAGCAAAGAGACACAGAA+AGG	+	chr7.4:10733960-10733979	MS.gene061869:CDS	45.0%
	AGGTGGAAAAGGTTTGGGAA+AGG	+	chr7.4:10733936-10733955	MS.gene061869:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.4:10734066-10734085	MS.gene061869:CDS	45.0%
	GGTGGAAAAGGTTTGGGAAA+GGG	+	chr7.4:10733937-10733956	MS.gene061869:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.4:10733982-10734001	MS.gene061869:CDS	45.0%
	TCAACCACCGAAACCGTAAA+GGG	-	chr7.4:10734211-10734230	None:intergenic	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.4:10734130-10734149	MS.gene061869:CDS	45.0%
!	GAGAGCATAGACAACATCCA+TGG	-	chr7.4:10734172-10734191	None:intergenic	45.0%
	ATGTCAGGTCGTGGAAAAGG+TGG	+	chr7.4:10733919-10733938	MS.gene061869:CDS	50.0%
	GAGAAAGACTGTTACCGCCA+TGG	+	chr7.4:10734152-10734171	MS.gene061869:CDS	50.0%
	GGAAAAGGTTTGGGAAAGGG+AGG	+	chr7.4:10733940-10733959	MS.gene061869:CDS	50.0%
	GGAAGGACCCTTTACGGTTT+CGG	+	chr7.4:10734201-10734220	MS.gene061869:CDS	50.0%
	GGTCGTGGAAAAGGTGGAAA+AGG	+	chr7.4:10733925-10733944	MS.gene061869:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+CGG	+	chr7.4:10734042-10734061	MS.gene061869:CDS	50.0%
	GTCTATGCTCTCAAGAGGCA+GGG	+	chr7.4:10734180-10734199	MS.gene061869:CDS	50.0%
	TGTCTATGCTCTCAAGAGGC+AGG	+	chr7.4:10734179-10734198	MS.gene061869:CDS	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.4:10734002-10734021	None:intergenic	50.0%
	AGGACCCTTTACGGTTTCGG+TGG	+	chr7.4:10734204-10734223	MS.gene061869:CDS	55.0%
	AGGCAGGGAAGGACCCTTTA+CGG	+	chr7.4:10734195-10734214	MS.gene061869:CDS	55.0%
	ATGCTCTCAAGAGGCAGGGA+AGG	+	chr7.4:10734184-10734203	MS.gene061869:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.4:10734021-10734040	MS.gene061869:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.4:10734016-10734035	MS.gene061869:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.4:10734018-10734037	None:intergenic	55.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.4:10734024-10734043	MS.gene061869:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10733919	10734230	10733919	ID=MS.gene061869
chr7.4	mRNA	10733919	10734230	10733919	ID=MS.gene061869.t1;Parent=MS.gene061869
chr7.4	exon	10733919	10734230	10733919	ID=MS.gene061869.t1.exon1;Parent=MS.gene061869.t1
chr7.4	CDS	10733919	10734230	10733919	ID=cds.MS.gene061869.t1;Parent=MS.gene061869.t1

Gene Sequence	>MS.gene061869 ATGTCAGGTCGTGGAAAAGGTGGAAAAGGTTTGGGAAAGGGAGGAGCAAAGAGACACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGCGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAAATCTTCTTGGAAAACGTGATTCGCGATGCTGTTACTTACACTGAGCACGCTAGGAGAAAGACTGTTACCGCCATGGATGTTGTCTATGCTCTCAAGAGGCAGGGAAGGACCCTTTACGGTTTCGGTGGTTGA
Protein sequence	>MS.gene061869.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG