AlfalfaGEDB | MS.gene31169

M. sativa cultivar XinJiangDaYe / MS.gene31169

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31169.t1	XP_003597343.1	98	153	3	0	1	153	1	153	5.90E-73	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31169.t1	Q2HU65	98.0	153	3	0	1	153	1	153	1.4e-75	283.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31169.t1	G7IHN1	98.0	153	3	0	1	153	1	153	4.2e-73	283.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene31169	0.8501	2.11E-60	-1.69E-46
MS.gene050352	MS.gene31169	0.806165	9.39E-50	-1.69E-46
MS.gene050689	MS.gene31169	0.801625	8.24E-49	-1.69E-46
MS.gene050901	MS.gene31169	0.862463	4.98E-64	-1.69E-46
MS.gene051247	MS.gene31169	0.823328	1.47E-53	-1.69E-46
MS.gene051328	MS.gene31169	0.819966	8.80E-53	-1.69E-46
MS.gene052516	MS.gene31169	0.80747	4.98E-50	-1.69E-46
MS.gene055466	MS.gene31169	0.812626	3.86E-51	-1.69E-46
MS.gene056215	MS.gene31169	0.872045	4.30E-67	-1.69E-46
MS.gene05622	MS.gene31169	0.826094	3.27E-54	-1.69E-46
MS.gene05623	MS.gene31169	0.807519	4.86E-50	-1.69E-46
MS.gene057693	MS.gene31169	0.807525	4.84E-50	-1.69E-46
MS.gene057694	MS.gene31169	0.80918	2.15E-50	-1.69E-46
MS.gene060429	MS.gene31169	0.838698	2.46E-57	-1.69E-46
MS.gene061176	MS.gene31169	0.805239	1.47E-49	-1.69E-46
MS.gene061177	MS.gene31169	0.803759	2.99E-49	-1.69E-46
MS.gene061178	MS.gene31169	0.805005	1.65E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31169.t1	MTR_2g096570	98.039	153	3	0	1	153	1	153	1.56e-103	293
MS.gene31169.t1	MTR_4g063280	93.333	135	6	2	19	153	14	145	4.03e-83	241
MS.gene31169.t1	MTR_4g071150	92.208	154	9	2	1	153	1	152	1.38e-81	237
MS.gene31169.t1	MTR_2g096610	88.889	153	12	2	1	153	1	148	2.88e-80	234
MS.gene31169.t1	MTR_4g063410	90.909	154	7	3	1	153	1	148	1.46e-77	227
MS.gene31169.t1	MTR_4g064005	90.260	154	8	3	1	153	1	148	2.66e-77	226
MS.gene31169.t1	MTR_4g064967	90.260	154	8	3	1	153	1	148	1.00e-76	225
MS.gene31169.t1	MTR_8g086640	97.727	132	2	1	1	131	1	132	5.02e-74	218
MS.gene31169.t1	MTR_7g113160	81.203	133	21	2	9	138	13	144	3.56e-71	211
MS.gene31169.t1	MTR_7g108320	75.000	128	26	3	3	129	6	128	6.26e-60	182
MS.gene31169.t1	MTR_7g093910	71.875	128	33	1	2	129	4	128	1.68e-59	181
MS.gene31169.t1	MTR_7g114060	73.438	128	29	2	3	129	2	125	2.87e-59	180
MS.gene31169.t1	MTR_7g114040	73.438	128	29	2	3	129	2	125	2.87e-59	180
MS.gene31169.t1	MTR_2g082510	74.790	119	30	0	10	128	3	121	1.61e-58	178
MS.gene31169.t1	MTR_2g082220	73.950	119	31	0	10	128	3	121	1.84e-57	176
MS.gene31169.t1	MTR_6g005150	64.754	122	29	3	23	138	26	139	1.63e-46	148
MS.gene31169.t1	MTR_4g063300	76.147	109	17	2	44	152	10	109	1.03e-45	145
MS.gene31169.t1	MTR_7g114050	68.644	118	22	3	16	129	10	116	1.34e-45	145
MS.gene31169.t1	MTR_1g011750	59.821	112	43	2	20	130	23	133	9.03e-36	120
MS.gene31169.t1	MTR_1g011850	59.821	112	43	2	20	130	23	133	9.03e-36	120
MS.gene31169.t1	MTR_1g114050	59.633	109	42	2	20	127	20	127	7.97e-35	118
MS.gene31169.t1	MTR_1g071040	61.538	104	38	2	25	127	28	130	3.74e-34	116
MS.gene31169.t1	MTR_1g069770	51.042	96	46	1	44	138	9	104	9.29e-27	97.1
MS.gene31169.t1	MTR_7g010440	70.588	51	15	0	77	127	122	172	1.63e-16	74.7
MS.gene31169.t1	MTR_4g005910	62.264	53	20	0	86	138	4	56	2.42e-16	68.9
MS.gene31169.t1	MTR_4g048490	43.810	105	30	2	23	127	26	101	4.14e-16	69.7
MS.gene31169.t1	MTR_7g027610	41.818	110	38	3	19	127	10	94	3.35e-15	67.8
MS.gene31169.t1	MTR_0653s0030	53.704	54	25	0	55	108	44	97	1.28e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31169.t1	AT5G02560	80.519	154	28	2	1	153	1	153	3.22e-78	229
MS.gene31169.t1	AT5G02560	69.663	178	28	3	1	153	1	177	2.29e-73	218
MS.gene31169.t1	AT5G27670	75.000	152	32	2	2	153	5	150	3.46e-73	216
MS.gene31169.t1	AT5G59870	73.377	154	36	2	1	153	1	150	1.36e-72	214
MS.gene31169.t1	AT1G51060	76.667	120	28	0	10	129	3	122	7.08e-61	184
MS.gene31169.t1	AT4G27230	73.984	123	32	0	10	132	3	125	1.33e-59	181
MS.gene31169.t1	AT4G27230	73.984	123	32	0	10	132	3	125	1.33e-59	181
MS.gene31169.t1	AT3G20670	71.318	129	37	0	10	138	3	131	2.90e-59	180
MS.gene31169.t1	AT5G54640	74.790	119	30	0	10	128	3	121	3.13e-58	177
MS.gene31169.t1	AT1G08880	72.222	126	32	1	7	129	3	128	3.56e-58	178
MS.gene31169.t1	AT1G54690	73.171	123	30	1	10	129	6	128	8.65e-58	177
MS.gene31169.t1	AT2G38810	59.821	112	43	2	20	130	25	135	2.00e-36	122
MS.gene31169.t1	AT2G38810	59.821	112	43	2	20	130	25	135	2.00e-36	122
MS.gene31169.t1	AT2G38810	59.821	112	43	2	20	130	25	135	2.00e-36	122
MS.gene31169.t1	AT2G38810	59.821	112	43	2	20	130	25	135	2.00e-36	122
MS.gene31169.t1	AT1G52740	61.905	105	38	2	24	127	27	130	1.31e-34	118
MS.gene31169.t1	AT3G54560	59.259	108	42	2	24	130	29	135	2.39e-34	117
MS.gene31169.t1	AT3G54560	59.259	108	42	2	24	130	29	135	2.39e-34	117
MS.gene31169.t1	AT4G13570	47.826	92	46	2	31	121	28	118	7.73e-20	79.7
MS.gene31169.t1	AT4G13570	47.253	91	46	2	32	121	35	124	1.69e-19	79.0

Find 47 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTAATCTAAGCCTTCTT+TGG	0.260442	2.4:-6825326	None:intergenic
GTAGACCTTTCAGTCTTCTT+AGG	0.271890	2.4:-6825254	None:intergenic
GTCTTCTTAGGCAAAAGAAC+AGG	0.334824	2.4:-6825242	None:intergenic
ACCAGCTCCTTTCTTCGTCT+TGG	0.348361	2.4:-6824799	None:intergenic
TTATGTTTAGGTTCTTGAAT+TGG	0.357612	2.4:+6825079	MS.gene31169:intron
AGAACAGCGGCGAGGTAAAC+AGG	0.381456	2.4:-6824954	None:intergenic
AAAAGAACAGGGTTAATGTT+TGG	0.382061	2.4:-6825230	None:intergenic
CAATTTCCAGTTGGAAGAAT+CGG	0.382886	2.4:+6824882	MS.gene31169:CDS
GCCTTAGGCTTAGGAGACTT+TGG	0.397268	2.4:-6825293	None:intergenic
GACTTTCCAGCCTTAGGCTT+AGG	0.400283	2.4:-6825302	None:intergenic
CTGGTCACAGACTTCTTCCT+TGG	0.400754	2.4:-6824840	None:intergenic
TTTGGAGACTTTCCAGCCTT+AGG	0.406671	2.4:-6825308	None:intergenic
GTTTAGGTTCTTGAATTGGC+TGG	0.434556	2.4:+6825083	MS.gene31169:intron
TAACGGCCGATTCTTCCAAC+TGG	0.469071	2.4:-6824888	None:intergenic
AAAGGAGCTGGTGGAAGGAA+AGG	0.477487	2.4:+6824810	MS.gene31169:CDS
AATTCCAAGGCACGTGTTGT+TGG	0.477559	2.4:+6825139	MS.gene31169:CDS
GGAGCTGGTGGAAGGAAAGG+TGG	0.481033	2.4:+6824813	MS.gene31169:CDS
GCAAGCACCAAGACGAAGAA+AGG	0.482883	2.4:+6824792	MS.gene31169:CDS
ACAGCCAACAACACGTGCCT+TGG	0.485674	2.4:-6825143	None:intergenic
AAGACCAGCTCTGATGGATC+TGG	0.491289	2.4:-6824859	None:intergenic
ATCTTCCTTTCTTCAAGTAA+CGG	0.498085	2.4:-6824905	None:intergenic
AAATTGAAGACCAGCTCTGA+TGG	0.498711	2.4:-6824865	None:intergenic
TACGCTCAACGTGTTGGTAC+TGG	0.502701	2.4:+6824927	MS.gene31169:CDS
TGTGAGAAATGATGAAGAGC+TGG	0.520971	2.4:+6825163	MS.gene31169:CDS
CACGTGATAACAAGAAGAAT+AGG	0.529318	2.4:+6825114	MS.gene31169:CDS
GGCTGGAAAGTCTCCAAAGA+AGG	0.542007	2.4:+6825313	MS.gene31169:CDS
AAGTCTCCTAAGCCTAAGGC+TGG	0.542736	2.4:+6825296	MS.gene31169:CDS
ATCGGCCGTTACTTGAAGAA+AGG	0.544708	2.4:+6824900	MS.gene31169:CDS
ACCAAAGTCTCCTAAGCCTA+AGG	0.550123	2.4:+6825292	MS.gene31169:CDS
GTGACCAGATCCATCAGAGC+TGG	0.553138	2.4:+6824855	MS.gene31169:CDS
CGAAGAAAGGAGCTGGTGGA+AGG	0.557443	2.4:+6824805	MS.gene31169:CDS
AAGGTCTACTAATGCTAGCA+AGG	0.572544	2.4:+6825268	MS.gene31169:CDS
ACCAAGACGAAGAAAGGAGC+TGG	0.579581	2.4:+6824798	MS.gene31169:CDS
GAGCTGGGAAAATTGTTAGC+TGG	0.589509	2.4:+6825179	MS.gene31169:CDS
GGAAGATACGCTCAACGTGT+TGG	0.597552	2.4:+6824921	MS.gene31169:CDS
GCTGGTCTTCAATTTCCAGT+TGG	0.598252	2.4:+6824873	MS.gene31169:CDS
GAAGGAAAGGTGGAGGACCA+AGG	0.610388	2.4:+6824823	MS.gene31169:CDS
TCTTCTTAGGCAAAAGAACA+GGG	0.618533	2.4:-6825241	None:intergenic
GCTGGTGGAAGGAAAGGTGG+AGG	0.624747	2.4:+6824816	MS.gene31169:CDS
AGAAGAATAGGATAATTCCA+AGG	0.643694	2.4:+6825126	MS.gene31169:CDS
GTGAGAAATGATGAAGAGCT+GGG	0.654622	2.4:+6825164	MS.gene31169:CDS
AAGACGAAGAAAGGAGCTGG+TGG	0.662071	2.4:+6824801	MS.gene31169:CDS
TCTTGAATATCTCGCTGCTG+AGG	0.665898	2.4:+6824974	MS.gene31169:CDS
AGCGAGATATTCAAGAACAG+CGG	0.706002	2.4:-6824967	None:intergenic
GATATTCAAGAACAGCGGCG+AGG	0.723455	2.4:-6824962	None:intergenic
GCTGGTGTAACAATTGCACA+TGG	0.750058	2.4:+6825197	MS.gene31169:CDS
GGTGTAACAATTGCACATGG+TGG	0.783361	2.4:+6825200	MS.gene31169:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTAAACATAAAAATAAAATT+AGG	-	chr2.4:6825069-6825088	None:intergenic	10.0%
!!!	CTAATTTTATTTTTATGTTT+AGG	+	chr2.4:6825067-6825086	MS.gene31169:intron	10.0%
!	AGGGTTACAAATTCAAAATT+AGG	-	chr2.4:6825049-6825068	None:intergenic	25.0%
!	CAAAATTAGGGTTACAAAAT+TGG	-	chr2.4:6825036-6825055	None:intergenic	25.0%
!	GGGTTACAAATTCAAAATTA+GGG	-	chr2.4:6825048-6825067	None:intergenic	25.0%
!!	TTATGTTTAGGTTCTTGAAT+TGG	+	chr2.4:6825079-6825098	MS.gene31169:intron	25.0%
	AAAAGAACAGGGTTAATGTT+TGG	-	chr2.4:6825233-6825252	None:intergenic	30.0%
	AATCAAAGATCATACGAAAG+AGG	-	chr2.4:6825012-6825031	None:intergenic	30.0%
	AGAAGAATAGGATAATTCCA+AGG	+	chr2.4:6825126-6825145	MS.gene31169:CDS	30.0%
	ATCTTCCTTTCTTCAAGTAA+CGG	-	chr2.4:6824908-6824927	None:intergenic	30.0%
	CAATTTCCAGTTGGAAGAAT+CGG	+	chr2.4:6824882-6824901	MS.gene31169:CDS	35.0%
	CACGTGATAACAAGAAGAAT+AGG	+	chr2.4:6825114-6825133	MS.gene31169:CDS	35.0%
	TCTTCTTAGGCAAAAGAACA+GGG	-	chr2.4:6825244-6825263	None:intergenic	35.0%
!	TTTTGCCTAAGAAGACTGAA+AGG	+	chr2.4:6825249-6825268	MS.gene31169:CDS	35.0%
	AAGGTCTACTAATGCTAGCA+AGG	+	chr2.4:6825268-6825287	MS.gene31169:CDS	40.0%
	AGCGAGATATTCAAGAACAG+CGG	-	chr2.4:6824970-6824989	None:intergenic	40.0%
	GTAGACCTTTCAGTCTTCTT+AGG	-	chr2.4:6825257-6825276	None:intergenic	40.0%
	GTCTTCTTAGGCAAAAGAAC+AGG	-	chr2.4:6825245-6825264	None:intergenic	40.0%
	GTGAGAAATGATGAAGAGCT+GGG	+	chr2.4:6825164-6825183	MS.gene31169:CDS	40.0%
	TGTGAGAAATGATGAAGAGC+TGG	+	chr2.4:6825163-6825182	MS.gene31169:CDS	40.0%
!	AAATTGAAGACCAGCTCTGA+TGG	-	chr2.4:6824868-6824887	None:intergenic	40.0%
!	GTTTAGGTTCTTGAATTGGC+TGG	+	chr2.4:6825083-6825102	MS.gene31169:intron	40.0%
	AATTCCAAGGCACGTGTTGT+TGG	+	chr2.4:6825139-6825158	MS.gene31169:CDS	45.0%
	ACCAAAGTCTCCTAAGCCTA+AGG	+	chr2.4:6825292-6825311	MS.gene31169:CDS	45.0%
	ATCGGCCGTTACTTGAAGAA+AGG	+	chr2.4:6824900-6824919	MS.gene31169:CDS	45.0%
	GAGCTGGGAAAATTGTTAGC+TGG	+	chr2.4:6825179-6825198	MS.gene31169:CDS	45.0%
	GCTGGTGTAACAATTGCACA+TGG	+	chr2.4:6825197-6825216	MS.gene31169:CDS	45.0%
	GGTGTAACAATTGCACATGG+TGG	+	chr2.4:6825200-6825219	MS.gene31169:CDS	45.0%
	TCTTGAATATCTCGCTGCTG+AGG	+	chr2.4:6824974-6824993	MS.gene31169:CDS	45.0%
	TTTGGAGACTTTCCAGCCTT+AGG	-	chr2.4:6825311-6825330	None:intergenic	45.0%
!!	GCTGGTCTTCAATTTCCAGT+TGG	+	chr2.4:6824873-6824892	MS.gene31169:CDS	45.0%
!!	TAAACATAAAAATAAAATTA+GGG	-	chr2.4:6825068-6825087	None:intergenic	5.0%
	AAAGGAGCTGGTGGAAGGAA+AGG	+	chr2.4:6824810-6824829	MS.gene31169:CDS	50.0%
	AAGACGAAGAAAGGAGCTGG+TGG	+	chr2.4:6824801-6824820	MS.gene31169:CDS	50.0%
	AAGTCTCCTAAGCCTAAGGC+TGG	+	chr2.4:6825296-6825315	MS.gene31169:CDS	50.0%
	ACCAAGACGAAGAAAGGAGC+TGG	+	chr2.4:6824798-6824817	MS.gene31169:CDS	50.0%
	ACCAGCTCCTTTCTTCGTCT+TGG	-	chr2.4:6824802-6824821	None:intergenic	50.0%
	CTGGTCACAGACTTCTTCCT+TGG	-	chr2.4:6824843-6824862	None:intergenic	50.0%
	GACTTTCCAGCCTTAGGCTT+AGG	-	chr2.4:6825305-6825324	None:intergenic	50.0%
	GATATTCAAGAACAGCGGCG+AGG	-	chr2.4:6824965-6824984	None:intergenic	50.0%
	GCAAGCACCAAGACGAAGAA+AGG	+	chr2.4:6824792-6824811	MS.gene31169:CDS	50.0%
	GCCTTAGGCTTAGGAGACTT+TGG	-	chr2.4:6825296-6825315	None:intergenic	50.0%
	GGAAGATACGCTCAACGTGT+TGG	+	chr2.4:6824921-6824940	MS.gene31169:CDS	50.0%
	GGCTGGAAAGTCTCCAAAGA+AGG	+	chr2.4:6825313-6825332	MS.gene31169:CDS	50.0%
!	AAGACCAGCTCTGATGGATC+TGG	-	chr2.4:6824862-6824881	None:intergenic	50.0%
!	TAACGGCCGATTCTTCCAAC+TGG	-	chr2.4:6824891-6824910	None:intergenic	50.0%
!	TACGCTCAACGTGTTGGTAC+TGG	+	chr2.4:6824927-6824946	MS.gene31169:CDS	50.0%
	ACAGCCAACAACACGTGCCT+TGG	-	chr2.4:6825146-6825165	None:intergenic	55.0%
	AGAACAGCGGCGAGGTAAAC+AGG	-	chr2.4:6824957-6824976	None:intergenic	55.0%
	CGAAGAAAGGAGCTGGTGGA+AGG	+	chr2.4:6824805-6824824	MS.gene31169:CDS	55.0%
	GAAGGAAAGGTGGAGGACCA+AGG	+	chr2.4:6824823-6824842	MS.gene31169:CDS	55.0%
	GTGACCAGATCCATCAGAGC+TGG	+	chr2.4:6824855-6824874	MS.gene31169:CDS	55.0%
	GCTGGTGGAAGGAAAGGTGG+AGG	+	chr2.4:6824816-6824835	MS.gene31169:CDS	60.0%
	GGAGCTGGTGGAAGGAAAGG+TGG	+	chr2.4:6824813-6824832	MS.gene31169:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	6824786	6825340	6824786	ID=MS.gene31169
chr2.4	mRNA	6824786	6825340	6824786	ID=MS.gene31169.t1;Parent=MS.gene31169
chr2.4	exon	6824786	6824995	6824786	ID=MS.gene31169.t1.exon1;Parent=MS.gene31169.t1
chr2.4	CDS	6824786	6824995	6824786	ID=cds.MS.gene31169.t1;Parent=MS.gene31169.t1
chr2.4	exon	6825089	6825340	6825089	ID=MS.gene31169.t1.exon2;Parent=MS.gene31169.t1
chr2.4	CDS	6825089	6825340	6825089	ID=cds.MS.gene31169.t1;Parent=MS.gene31169.t1

Gene Sequence

>MS.gene31169

ATGGACGCAAGCACCAAGACGAAGAAAGGAGCTGGTGGAAGGAAAGGTGGAGGACCAAGGAAGAAGTCTGTGACCAGATCCATCAGAGCTGGTCTTCAATTTCCAGTTGGAAGAATCGGCCGTTACTTGAAGAAAGGAAGATACGCTCAACGTGTTGGTACTGGTGCTCCTGTTTACCTCGCCGCTGTTCTTGAATATCTCGCTGCTGAGGTGAATTTCAAATCCTCTTTCGTATGATCTTTGATTTCCAATTTTGTAACCCTAATTTTGAATTTGTAACCCTAATTTTATTTTTATGTTTAGGTTCTTGAATTGGCTGGAAATGCTGCACGTGATAACAAGAAGAATAGGATAATTCCAAGGCACGTGTTGTTGGCTGTGAGAAATGATGAAGAGCTGGGAAAATTGTTAGCTGGTGTAACAATTGCACATGGTGGTGTTCTTCCAAACATTAACCCTGTTCTTTTGCCTAAGAAGACTGAAAGGTCTACTAATGCTAGCAAGGAACCAAAGTCTCCTAAGCCTAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene31169.t1

MDASTKTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPVLLPKKTERSTNASKEPKSPKPKAGKSPKKA