Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055875 | MS.gene057756 | 0.803674 | 3.12E-49 | -1.69E-46 |
| MS.gene057656 | MS.gene057756 | 0.947026 | 1.49E-105 | -1.69E-46 |
| MS.gene057755 | MS.gene057756 | 0.884741 | 1.48E-71 | -1.69E-46 |
| MS.gene057756 | MS.gene057757 | 0.831908 | 1.28E-55 | -1.69E-46 |
| MS.gene057756 | MS.gene057760 | 0.880645 | 4.63E-70 | -1.69E-46 |
| MS.gene057756 | MS.gene057763 | 0.803697 | 3.08E-49 | -1.69E-46 |
| MS.gene057756 | MS.gene060167 | 0.807464 | 4.99E-50 | -1.69E-46 |
| MS.gene057756 | MS.gene34700 | 0.823602 | 1.27E-53 | -1.69E-46 |
| MS.gene057756 | MS.gene48704 | 0.808065 | 3.72E-50 | -1.69E-46 |
| MS.gene057756 | MS.gene88091 | 0.803707 | 3.07E-49 | -1.69E-46 |
| MS.gene057756 | MS.gene88112 | 0.810217 | 1.29E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCCGTCCGTCGAGGAATA+TGG | 0.196755 | 4.4:+36313460 | MS.gene057756:intron |
| AGTCCGTCCGTCGAGGAATA+TGG | 0.196755 | 4.4:+36314618 | MS.gene057756:intron |
| TTCACTACGCACTCTACTTT+TGG | 0.220660 | 4.4:-36312759 | None:intergenic |
| TTCACTACGCACTCTACTTT+TGG | 0.262170 | 4.4:-36313934 | None:intergenic |
| CCCGTTCAGTACTGGGAATA+TGG | 0.302089 | 4.4:+36317221 | MS.gene057756:CDS |
| GATGGTGTTCAGCATGATTA+TGG | 0.327272 | 4.4:+36310399 | MS.gene057756:CDS |
| TGGGAGATATGACAGATCTA+TGG | 0.334089 | 4.4:+36313480 | MS.gene057756:CDS |
| TGGGAGATATGACAGATCTA+TGG | 0.334089 | 4.4:+36314638 | MS.gene057756:CDS |
| GTGATTGATACGAGTGGATT+TGG | 0.349177 | 4.4:+36310509 | MS.gene057756:intron |
| TCCGGTTCGACCAGCCTGTT+TGG | 0.390434 | 4.4:+36317531 | MS.gene057756:CDS |
| TCTCCCAACCCGTTCAGTAC+TGG | 0.409727 | 4.4:+36317213 | MS.gene057756:CDS |
| ACCAAACAGGCTGGTCGAAC+CGG | 0.411099 | 4.4:-36317532 | None:intergenic |
| TTTGTACCTGATTTGTTGAA+TGG | 0.411582 | 4.4:-36310551 | None:intergenic |
| TCAAACTTACTTTCCATGTC+TGG | 0.416082 | 4.4:-36317703 | None:intergenic |
| GTCCGTCCGTCGAGGAATAT+GGG | 0.422376 | 4.4:+36313461 | MS.gene057756:intron |
| GTCCGTCCGTCGAGGAATAT+GGG | 0.422376 | 4.4:+36314619 | MS.gene057756:intron |
| GACCGGATTGTTTACCCCTC+CGG | 0.424311 | 4.4:+36317513 | MS.gene057756:CDS |
| AAGATTAACAAAGTTGCTGT+TGG | 0.428899 | 4.4:+36310348 | None:intergenic |
| CTCCCAACCCGTTCAGTACT+GGG | 0.443973 | 4.4:+36317214 | MS.gene057756:CDS |
| AGTGGATTTGGTAGGCAATA+CGG | 0.465499 | 4.4:+36310521 | MS.gene057756:CDS |
| TTCCCAGTACTGAACGGGTT+GGG | 0.465952 | 4.4:-36317216 | None:intergenic |
| CCATATTCCCAGTACTGAAC+GGG | 0.471927 | 4.4:-36317221 | None:intergenic |
| AAGTTGCCATTCAACAAATC+AGG | 0.472764 | 4.4:+36310545 | MS.gene057756:CDS |
| GTGGATTTGGTAGGCAATAC+GGG | 0.495201 | 4.4:+36310522 | MS.gene057756:CDS |
| AACCGGAGGGGTAAACAATC+CGG | 0.519027 | 4.4:-36317515 | None:intergenic |
| TAACAAAGTTGCTGTTGGAT+CGG | 0.524007 | 4.4:+36310353 | MS.gene057756:CDS |
| TCAAATCGCCCTTCCTTCGA+TGG | 0.536762 | 4.4:+36310381 | MS.gene057756:CDS |
| GGGAGATATGACAGATCTAT+GGG | 0.538121 | 4.4:+36313481 | MS.gene057756:CDS |
| GGGAGATATGACAGATCTAT+GGG | 0.538121 | 4.4:+36314639 | MS.gene057756:CDS |
| TCCATATTCCCAGTACTGAA+CGG | 0.550792 | 4.4:-36317222 | None:intergenic |
| AGTAGAACTTACAACGCATC+GGG | 0.554568 | 4.4:-36312788 | None:intergenic |
| AGTAGAACTTACAACGCATC+GGG | 0.554568 | 4.4:-36313963 | None:intergenic |
| TGCTGAACACCATCGAAGGA+AGG | 0.554944 | 4.4:-36310390 | None:intergenic |
| TTGATACGAGTGGATTTGGT+AGG | 0.557534 | 4.4:+36310513 | MS.gene057756:CDS |
| TTCAGTACTGGGAATATGGA+TGG | 0.571027 | 4.4:+36317225 | MS.gene057756:CDS |
| CAGTAGAACTTACAACGCAT+CGG | 0.573810 | 4.4:-36313964 | None:intergenic |
| GCTGAACACCATCGAAGGAA+GGG | 0.586274 | 4.4:-36310389 | None:intergenic |
| AACAGGCTGGTCGAACCGGA+GGG | 0.587783 | 4.4:-36317528 | None:intergenic |
| GAGTAGAACTTACAACGCAT+CGG | 0.590910 | 4.4:-36312789 | None:intergenic |
| CTCCCATATTCCTCGACGGA+CGG | 0.592180 | 4.4:-36313463 | None:intergenic |
| CTCCCATATTCCTCGACGGA+CGG | 0.592180 | 4.4:-36314621 | None:intergenic |
| AACGCGTCGGTGAACACGCA+AGG | 0.594274 | 4.4:-36317667 | None:intergenic |
| ATTCCCAGTACTGAACGGGT+TGG | 0.599227 | 4.4:-36317217 | None:intergenic |
| AAACAGGCTGGTCGAACCGG+AGG | 0.601388 | 4.4:-36317529 | None:intergenic |
| ATCATGCTGAACACCATCGA+AGG | 0.614724 | 4.4:-36310394 | None:intergenic |
| AATAAACAGTCCGTCCGTCG+AGG | 0.617555 | 4.4:+36313453 | MS.gene057756:intron |
| AATAAACAGTCCGTCCGTCG+AGG | 0.617555 | 4.4:+36314611 | MS.gene057756:intron |
| TATTGCTGAGAAGCCAGACA+TGG | 0.619519 | 4.4:+36317690 | MS.gene057756:CDS |
| TATTCAGTGATTGATACGAG+TGG | 0.626968 | 4.4:+36310503 | MS.gene057756:intron |
| GAACTTACAACGCATCGGGT+CGG | 0.631203 | 4.4:-36312784 | None:intergenic |
| GAACTTACAACGCATCGGGT+CGG | 0.631203 | 4.4:-36313959 | None:intergenic |
| AAAACAATGAACAGAAGTTG+CGG | 0.642478 | 4.4:+36317164 | MS.gene057756:intron |
| CTTCTCAGCAATAAACGCGT+CGG | 0.647614 | 4.4:-36317680 | None:intergenic |
| ACAGGCTGGTCGAACCGGAG+GGG | 0.658075 | 4.4:-36317527 | None:intergenic |
| ATATCTCCCATATTCCTCGA+CGG | 0.684817 | 4.4:-36313467 | None:intergenic |
| ATATCTCCCATATTCCTCGA+CGG | 0.684817 | 4.4:-36314625 | None:intergenic |
| ACAATGAACAGATGTTACTG+AGG | 0.694420 | 4.4:+36317800 | MS.gene057756:intron |
| ACAATGAACAGAAGTTGCGG+CGG | 0.700063 | 4.4:+36317167 | MS.gene057756:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 36310351 | 36317833 | 36310351 | ID=MS.gene057756 |
| chr4.4 | mRNA | 36310351 | 36317833 | 36310351 | ID=MS.gene057756.t1;Parent=MS.gene057756 |
| chr4.4 | exon | 36310351 | 36310420 | 36310351 | ID=MS.gene057756.t1.exon1;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36310351 | 36310420 | 36310351 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36310510 | 36310566 | 36310510 | ID=MS.gene057756.t1.exon2;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36310510 | 36310566 | 36310510 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36312745 | 36312798 | 36312745 | ID=MS.gene057756.t1.exon3;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36312745 | 36312798 | 36312745 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36313462 | 36313539 | 36313462 | ID=MS.gene057756.t1.exon4;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36313462 | 36313539 | 36313462 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36313920 | 36313973 | 36313920 | ID=MS.gene057756.t1.exon5;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36313920 | 36313973 | 36313920 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36314620 | 36314697 | 36314620 | ID=MS.gene057756.t1.exon6;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36314620 | 36314697 | 36314620 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36317178 | 36317246 | 36317178 | ID=MS.gene057756.t1.exon7;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36317178 | 36317246 | 36317178 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36317505 | 36317552 | 36317505 | ID=MS.gene057756.t1.exon8;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36317505 | 36317552 | 36317505 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36317635 | 36317715 | 36317635 | ID=MS.gene057756.t1.exon9;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36317635 | 36317715 | 36317635 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| chr4.4 | exon | 36317811 | 36317833 | 36317811 | ID=MS.gene057756.t1.exon10;Parent=MS.gene057756.t1 |
| chr4.4 | CDS | 36317811 | 36317833 | 36317811 | ID=cds.MS.gene057756.t1;Parent=MS.gene057756.t1 |
| Gene Sequence |
| Protein sequence |
