AlfalfaGEDB | MS.gene48704

M. sativa cultivar XinJiangDaYe / MS.gene48704

KEGG: -
GO: GO:0006629 GO:0008150 GO:0008152 GO:0044238 GO:0071704
PFAM: PF01764.28

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48704.t1	XP_003607601.1	84.3	286	13	1	44	297	128	413	2.80E-119	438.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48704.t1	Q9SJI7	49.0	296	113	3	35	297	95	385	2.1e-70	267.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48704.t1	G7JCM9	84.3	286	13	1	44	297	128	413	2.0e-119	438.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050026	MS.gene48704	0.801991	6.93E-49	-1.69E-46
MS.gene050430	MS.gene48704	0.803277	3.76E-49	-1.69E-46
MS.gene050442	MS.gene48704	0.809224	2.10E-50	-1.69E-46
MS.gene050750	MS.gene48704	0.818035	2.42E-52	-1.69E-46
MS.gene050807	MS.gene48704	0.802177	6.35E-49	-1.69E-46
MS.gene050820	MS.gene48704	0.812541	4.03E-51	-1.69E-46
MS.gene050953	MS.gene48704	0.809386	1.94E-50	-1.69E-46
MS.gene051265	MS.gene48704	0.841793	3.83E-58	-1.69E-46
MS.gene052333	MS.gene48704	0.807715	4.41E-50	-1.69E-46
MS.gene052867	MS.gene48704	0.832882	7.34E-56	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48704.t1	MTR_4g080100	77.848	316	37	2	14	297	99	413	1.61e-168	475
MS.gene48704.t1	MTR_2g015690	40.411	292	113	4	44	297	122	390	3.87e-60	197
MS.gene48704.t1	MTR_4g087830	33.219	292	128	5	44	297	107	369	6.18e-43	152
MS.gene48704.t1	MTR_4g087810	32.877	292	129	6	44	297	116	378	8.06e-41	146
MS.gene48704.t1	MTR_7g096120	31.715	309	127	7	45	295	174	456	4.80e-37	138
MS.gene48704.t1	MTR_7g096140	30.844	308	131	7	45	295	174	456	2.49e-36	135
MS.gene48704.t1	MTR_4g077070	30.000	320	135	7	37	295	188	479	5.95e-33	127
MS.gene48704.t1	MTR_7g006400	38.961	154	64	5	51	201	199	325	1.48e-21	94.7
MS.gene48704.t1	MTR_7g081620	34.194	155	77	5	48	196	169	304	3.59e-16	79.0
MS.gene48704.t1	MTR_1995s0010	33.588	131	62	2	68	198	1	106	2.12e-13	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48704.t1	AT2G42690	48.726	314	116	5	21	298	82	386	4.41e-92	279
MS.gene48704.t1	AT4G18550	36.304	303	128	7	35	297	113	390	4.40e-50	171
MS.gene48704.t1	AT4G18550	36.304	303	128	7	35	297	130	407	4.69e-50	171
MS.gene48704.t1	AT1G06250	34.495	287	126	4	48	295	111	374	3.51e-43	153
MS.gene48704.t1	AT1G06250	34.276	283	124	4	52	295	1	260	1.53e-41	146
MS.gene48704.t1	AT1G06800	30.892	314	126	9	45	295	184	469	1.18e-32	126
MS.gene48704.t1	AT4G18550	40.361	166	74	3	35	200	130	270	4.04e-32	121
MS.gene48704.t1	AT2G30550	28.435	313	134	8	45	295	200	484	7.83e-30	118
MS.gene48704.t1	AT1G30370	26.885	305	144	6	44	296	212	489	1.35e-25	106
MS.gene48704.t1	AT2G31690	31.841	201	106	4	46	240	185	360	1.20e-23	100

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Find 76 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACAGTGTAGTGTGTA+GGG	0.515742	4.4:-71714858	None:intergenic
AACAAACCAGAAGCAGATTC+AGG	0.440065	4.4:-71715091	None:intergenic
AACCAACCCTTCATTACTTT+AGG	0.305402	4.4:-71715523	None:intergenic
AACCAATCCAGTTAGACTCA+CGG	0.662669	4.4:-71714955	None:intergenic
AATCCAAGTGTGGTATTAGT+AGG	0.433511	4.4:+71715652	MS.gene48704:CDS
AATTGTGAGTGCGTTTGATT+TGG	0.435943	4.4:+71715699	MS.gene48704:CDS
ACATTATCCAGGGAAGTTAT+TGG	0.290351	4.4:+71715865	MS.gene48704:intron
ACCAACCCTTCATTACTTTA+GGG	0.280032	4.4:-71715522	None:intergenic
AGAGTCCCTAAAGTAATGAA+GGG	0.619750	4.4:+71715517	MS.gene48704:CDS
AGCTTTATTCCCAACTTGCG+GGG	0.579031	4.4:-71715771	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTTATACAAACTCACC+TGG	-	chr4.4:71715256-71715275	None:intergenic	30.0%
ATCTTATACCACATTATCCA+GGG	+	chr4.4:71715855-71715874	MS.gene48704:intron	30.0%
CTCCCAAACAACAATAATAA+GGG	+	chr4.4:71715142-71715161	MS.gene48704:CDS	30.0%
GGAATAAAGCTTTCAATGAT+AGG	+	chr4.4:71715783-71715802	MS.gene48704:intron	30.0%
AAACAACAGTGTAGTGTGTA+GGG	-	chr4.4:71714861-71714880	None:intergenic	35.0%
AACCAACCCTTCATTACTTT+AGG	-	chr4.4:71715526-71715545	None:intergenic	35.0%
AATAACTTCCCTGGATAATG+TGG	-	chr4.4:71715866-71715885	None:intergenic	35.0%
AATCCAAGTGTGGTATTAGT+AGG	+	chr4.4:71715652-71715671	MS.gene48704:CDS	35.0%
AATTGTGAGTGCGTTTGATT+TGG	+	chr4.4:71715699-71715718	MS.gene48704:CDS	35.0%
ACATTATCCAGGGAAGTTAT+TGG	+	chr4.4:71715865-71715884	MS.gene48704:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	71714818	71716135	71714818	ID=MS.gene48704
chr4.4	mRNA	71714818	71716135	71714818	ID=MS.gene48704.t1;Parent=MS.gene48704
chr4.4	exon	71714818	71715163	71714818	ID=MS.gene48704.t1.exon1;Parent=MS.gene48704.t1
chr4.4	CDS	71714818	71715163	71714818	ID=cds.MS.gene48704.t1;Parent=MS.gene48704.t1
chr4.4	exon	71715479	71715778	71715479	ID=MS.gene48704.t1.exon2;Parent=MS.gene48704.t1
chr4.4	CDS	71715479	71715778	71715479	ID=cds.MS.gene48704.t1;Parent=MS.gene48704.t1
chr4.4	exon	71715876	71716135	71715876	ID=MS.gene48704.t1.exon3;Parent=MS.gene48704.t1
chr4.4	CDS	71715876	71716135	71715876	ID=cds.MS.gene48704.t1;Parent=MS.gene48704.t1

Gene Sequence

>MS.gene48704

ATGGAAAAACCTCATTCTTCAACAAAGTCATGCTTGAAAACCCTACACACTACACTGTTGTTTCTTTCCTCTACGCTACTGCACGTGTATCTGTTCCTGAAGCTTTTATTCTTCACTCACTATCACGTGAGTTGGGACCGTGAGTCTAACTGGATTGGTTACATCGCTGTTAGTTCCGATGAACGGAGTAGAGAGTTGGGTCGTCGTGAAATATATGTTGTGTGGCGTGGTACTACAAGGGACCTTGAATGGATTAACGTGTTTGGTGCTGCTCCTGAATCTGCTTCTGGTTTGTTGAGTGCTAAAAGTCTTCGTGAGTTCAACCTCCCAAACAACAATAATAAGGGTACTCAATCTTCTACTATAAGTTTTGATATAATTATCTTTAACTCCATATTCATTTTTAGATTTTACATAAACATACTTAAACTGATTCCAGGTGAGTTTGTATAAGTTTTACGAAGAAGTAAATGTAATTGAACTGAGTTTATATAAACGTATATGAAGTGAGTATGCATAAGATCTAAAAAATAAGAATTTCGAATGAATTGAGTTTACATTAATGTACTTTAACTGAATTTTTGCTCTAAATTTTAATTAACCATTTATAAAGTATATGTAACGAATTAAATTAGAATGTAATTTTTTGTTTATGTTACAGATGACAATAGCAGCAGCAGTGACGATGAGGATGACAAGAGAGTCCCTAAAGTAATGAAGGGTTGGTTGACAATCTACACCTCAGACGACCCAAAATCTCCTTTCACAAAAACAAGCGTGAGAACACAGGTTCTAAACATAGTAAAAAGTTTGTTGAACAAATACAAGAATGAAAATCCAAGTGTGGTATTAGTAGGACATAGTCTAGGTGCAAGTTTGTCAATTGTGAGTGCGTTTGATTTGGTGGAGAACGGTGTAACAAACGTTCCCGTTTACTGCATTTGTGTTTGGGTCCCCGCAAGTTGGGAATAAAGCTTTCAATGATAGGTTCAAAAAGTTTCAAAATTTGAAGGTTTTACATGTGAGGAATGTGATTGATCTTATACCACATTATCCAGGGAAGTTATTGGGGTATGAGTATACAGGGACAGAGTTGGTGATTGATACAAGAAAGTCGACGAGTTTGAAAGATTCGAAGAATCCTAGTGATTGGCATAATTTGCAAGCTATGTTGCATATTGTGGCTGGTTGGAATGGGAGTGATGGGGAGTTTGAGGTGAAGGTGAAGAGGAGTTTGGCTTTAGTGAATAAGTCTTGTGATTACTTGAAGGAAGAGTGTCACGTGCCGGCGTCATGGTGGGTTAGGTATGGTGCGTAG

Protein sequence

>MS.gene48704.t1

MEKPHSSTKSCLKTLHTTLLFLSSTLLHVYLFLKLLFFTHYHVSWDRESNWIGYIAVSSDERSRELGRREIYVVWRGTTRDLEWINVFGAAPESASGLLSAKSLREFNLPNNNNKDDNSSSSDDEDDKRVPKVMKGWLTIYTSDDPKSPFTKTSVRTQVLNIVKSLLNKYKNENPSVVLVGHSLGASLSIVSAFDLVENGVTNVPVYCICVWVPARKLLGYEYTGTELVIDTRKSTSLKDSKNPSDWHNLQAMLHIVAGWNGSDGEFEVKVKRSLALVNKSCDYLKEECHVPASWWVRYGA