AlfalfaGEDB | MS.gene52525

M. sativa cultivar XinJiangDaYe / MS.gene52525

KEGG: -
GO: -
PFAM: PF03004.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52525.t1	XP_024631911.1	93.9	115	7	0	1	115	416	530	2.20E-52	214.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52525.t1	A0A3Q7XGP6	68.7	134	34	3	1	133	137	263	2.1e-36	161.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene52525	0.826559	2.54E-54	-1.69E-46
MS.gene049404	MS.gene52525	0.802283	6.04E-49	-1.69E-46
MS.gene049435	MS.gene52525	0.816569	5.17E-52	-1.69E-46
MS.gene049452	MS.gene52525	0.801511	8.70E-49	-1.69E-46
MS.gene049501	MS.gene52525	0.813879	2.05E-51	-1.69E-46
MS.gene049669	MS.gene52525	0.810901	9.15E-51	-1.69E-46
MS.gene050021	MS.gene52525	0.82028	7.46E-53	-1.69E-46
MS.gene050026	MS.gene52525	0.867885	9.84E-66	-1.69E-46
MS.gene050355	MS.gene52525	0.804438	2.16E-49	-1.69E-46
MS.gene050442	MS.gene52525	0.81879	1.63E-52	-1.69E-46
MS.gene050750	MS.gene52525	0.837657	4.57E-57	-1.69E-46
MS.gene050953	MS.gene52525	0.840798	7.00E-58	-1.69E-46
MS.gene051181	MS.gene52525	0.816879	4.41E-52	-1.69E-46
MS.gene051198	MS.gene52525	0.814016	1.91E-51	-1.69E-46
MS.gene05125	MS.gene52525	0.81396	1.96E-51	-1.69E-46
MS.gene051265	MS.gene52525	0.825502	4.53E-54	-1.69E-46
MS.gene051279	MS.gene52525	0.821027	5.02E-53	-1.69E-46
MS.gene051594	MS.gene52525	0.813837	2.09E-51	-1.69E-46
MS.gene051710	MS.gene52525	0.826153	3.17E-54	-1.69E-46
MS.gene051744	MS.gene52525	0.817307	3.53E-52	-1.69E-46
MS.gene051800	MS.gene52525	0.80707	6.05E-50	-1.69E-46
MS.gene052042	MS.gene52525	0.801211	1.00E-48	-1.69E-46
MS.gene052190	MS.gene52525	0.825387	4.82E-54	-1.69E-46
MS.gene052333	MS.gene52525	0.826099	3.27E-54	-1.69E-46
MS.gene052447	MS.gene52525	0.81696	4.22E-52	-1.69E-46
MS.gene052488	MS.gene52525	0.808837	2.54E-50	-1.69E-46
MS.gene052519	MS.gene52525	0.829247	5.73E-55	-1.69E-46
MS.gene052582	MS.gene52525	0.82178	3.37E-53	-1.69E-46
MS.gene052584	MS.gene52525	0.823208	1.57E-53	-1.69E-46
MS.gene052773	MS.gene52525	0.80284	4.64E-49	-1.69E-46
MS.gene052863	MS.gene52525	0.801471	8.86E-49	-1.69E-46
MS.gene052867	MS.gene52525	0.832305	1.02E-55	-1.69E-46
MS.gene053164	MS.gene52525	0.80508	1.59E-49	-1.69E-46
MS.gene053166	MS.gene52525	0.816435	5.54E-52	-1.69E-46
MS.gene053201	MS.gene52525	0.81996	8.83E-53	-1.69E-46
MS.gene053512	MS.gene52525	0.814549	1.46E-51	-1.69E-46
MS.gene053621	MS.gene52525	0.810907	9.13E-51	-1.69E-46
MS.gene053883	MS.gene52525	0.800449	1.43E-48	-1.69E-46
MS.gene053966	MS.gene52525	0.80755	4.79E-50	-1.69E-46
MS.gene053998	MS.gene52525	0.814991	1.16E-51	-1.69E-46
MS.gene05414	MS.gene52525	0.82931	5.53E-55	-1.69E-46
MS.gene05415	MS.gene52525	0.839955	1.16E-57	-1.69E-46
MS.gene054160	MS.gene52525	0.813822	2.11E-51	-1.69E-46
MS.gene05417	MS.gene52525	0.816699	4.83E-52	-1.69E-46
MS.gene054236	MS.gene52525	0.84193	3.52E-58	-1.69E-46
MS.gene05423	MS.gene52525	0.827973	1.16E-54	-1.69E-46
MS.gene054291	MS.gene52525	-0.851999	6.15E-61	-1.69E-46
MS.gene05435	MS.gene52525	0.815574	8.62E-52	-1.69E-46
MS.gene054405	MS.gene52525	0.838698	2.46E-57	-1.69E-46
MS.gene054467	MS.gene52525	0.84367	1.21E-58	-1.69E-46
MS.gene054468	MS.gene52525	0.816199	6.25E-52	-1.69E-46
MS.gene054559	MS.gene52525	0.819892	9.15E-53	-1.69E-46
MS.gene054651	MS.gene52525	0.817143	3.84E-52	-1.69E-46
MS.gene054775	MS.gene52525	0.819893	9.14E-53	-1.69E-46
MS.gene054801	MS.gene52525	0.814093	1.84E-51	-1.69E-46
MS.gene055056	MS.gene52525	0.804173	2.45E-49	-1.69E-46
MS.gene055227	MS.gene52525	0.814388	1.58E-51	-1.69E-46
MS.gene055228	MS.gene52525	0.822727	2.03E-53	-1.69E-46
MS.gene055356	MS.gene52525	0.811572	6.55E-51	-1.69E-46
MS.gene055357	MS.gene52525	0.8098	1.58E-50	-1.69E-46
MS.gene055586	MS.gene52525	0.802269	6.08E-49	-1.69E-46
MS.gene055589	MS.gene52525	0.807228	5.60E-50	-1.69E-46
MS.gene055725	MS.gene52525	0.821497	3.91E-53	-1.69E-46
MS.gene055778	MS.gene52525	0.817687	2.90E-52	-1.69E-46
MS.gene055818	MS.gene52525	0.853747	1.95E-61	-1.69E-46
MS.gene055875	MS.gene52525	0.83661	8.46E-57	-1.69E-46
MS.gene055926	MS.gene52525	0.802699	4.96E-49	-1.69E-46
MS.gene05604	MS.gene52525	0.80846	3.06E-50	-1.69E-46
MS.gene056162	MS.gene52525	0.810687	1.02E-50	-1.69E-46
MS.gene056173	MS.gene52525	0.820786	5.71E-53	-1.69E-46
MS.gene056174	MS.gene52525	0.858629	7.23E-63	-1.69E-46
MS.gene056252	MS.gene52525	0.806392	8.41E-50	-1.69E-46
MS.gene056366	MS.gene52525	0.813419	2.58E-51	-1.69E-46
MS.gene056498	MS.gene52525	0.819832	9.44E-53	-1.69E-46
MS.gene056506	MS.gene52525	0.812896	3.37E-51	-1.69E-46
MS.gene05690	MS.gene52525	0.803093	4.11E-49	-1.69E-46
MS.gene057021	MS.gene52525	0.801489	8.79E-49	-1.69E-46
MS.gene057026	MS.gene52525	0.807011	6.23E-50	-1.69E-46
MS.gene057194	MS.gene52525	0.806443	8.21E-50	-1.69E-46
MS.gene057314	MS.gene52525	0.800979	1.12E-48	-1.69E-46
MS.gene057414	MS.gene52525	0.809859	1.54E-50	-1.69E-46
MS.gene057444	MS.gene52525	0.832133	1.12E-55	-1.69E-46
MS.gene057598	MS.gene52525	0.811447	6.97E-51	-1.69E-46
MS.gene057755	MS.gene52525	0.805423	1.34E-49	-1.69E-46
MS.gene058010	MS.gene52525	0.823028	1.73E-53	-1.69E-46
MS.gene058037	MS.gene52525	0.834119	3.60E-56	-1.69E-46
MS.gene058294	MS.gene52525	0.817896	2.60E-52	-1.69E-46
MS.gene058515	MS.gene52525	0.845254	4.56E-59	-1.69E-46
MS.gene058625	MS.gene52525	0.81836	2.04E-52	-1.69E-46
MS.gene058979	MS.gene52525	0.825305	5.04E-54	-1.69E-46
MS.gene059084	MS.gene52525	0.800905	1.16E-48	-1.69E-46
MS.gene059128	MS.gene52525	0.808686	2.74E-50	-1.69E-46
MS.gene05925	MS.gene52525	0.813372	2.65E-51	-1.69E-46
MS.gene059272	MS.gene52525	0.847412	1.18E-59	-1.69E-46
MS.gene059276	MS.gene52525	0.833298	5.78E-56	-1.69E-46
MS.gene059382	MS.gene52525	0.821772	3.38E-53	-1.69E-46
MS.gene059458	MS.gene52525	0.823565	1.29E-53	-1.69E-46
MS.gene059459	MS.gene52525	0.838151	3.41E-57	-1.69E-46
MS.gene059460	MS.gene52525	0.830291	3.19E-55	-1.69E-46
MS.gene059550	MS.gene52525	0.806303	8.78E-50	-1.69E-46
MS.gene059557	MS.gene52525	0.810286	1.24E-50	-1.69E-46
MS.gene059584	MS.gene52525	0.880552	5.00E-70	-1.69E-46
MS.gene059625	MS.gene52525	0.824846	6.47E-54	-1.69E-46
MS.gene060011	MS.gene52525	0.852888	3.44E-61	-1.69E-46
MS.gene060167	MS.gene52525	0.868369	6.87E-66	-1.69E-46
MS.gene060310	MS.gene52525	0.806931	6.47E-50	-1.69E-46
MS.gene060322	MS.gene52525	0.829047	6.40E-55	-1.69E-46
MS.gene06052	MS.gene52525	0.830486	2.86E-55	-1.69E-46
MS.gene060553	MS.gene52525	0.807024	6.19E-50	-1.69E-46
MS.gene060919	MS.gene52525	0.856682	2.73E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52525.t1	MTR_0125s0060	92.391	92	7	0	39	130	1	92	1.01e-55	170
MS.gene52525.t1	MTR_2g035110	80.435	92	18	0	39	130	1	92	5.18e-45	143
MS.gene52525.t1	MTR_4g012560	81.395	43	3	1	86	128	24	61	1.61e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACCCATCTGGTTTCCCTTT+TGG	0.170423	7.2:+62808779	None:intergenic
AACAAGAAGGGGCACATATT+TGG	0.220030	7.2:-62808536	MS.gene52525:CDS
AATTGACAATGATATTTATA+TGG	0.247121	7.2:-62808576	MS.gene52525:CDS
TTCTACCGATGTAGCTTCTT+CGG	0.360773	7.2:-62808459	MS.gene52525:CDS
AAGGGGCACATATTTGGATT+AGG	0.362998	7.2:-62808530	MS.gene52525:CDS
GTTTCTCAAACGTTTGTGTT+TGG	0.390866	7.2:+62808642	None:intergenic
GATATTTATATGGAAATTGT+TGG	0.391812	7.2:-62808566	MS.gene52525:CDS
GACAAACTTCATAGACCAAA+AGG	0.414225	7.2:-62808794	MS.gene52525:CDS
GTTTCATTAAACCACCCATC+TGG	0.467331	7.2:+62808767	None:intergenic
TTAGAGAGTGGAATTGGATA+GGG	0.471643	7.2:-62808840	MS.gene52525:intron
TTTAGAGAGTGGAATTGGAT+AGG	0.476342	7.2:-62808841	MS.gene52525:intron
TGTTGGAGGCATTAACAAGA+AGG	0.515610	7.2:-62808549	MS.gene52525:CDS
ATTTATATGGAAATTGTTGG+AGG	0.522832	7.2:-62808563	MS.gene52525:CDS
TCATTGTCAATTTCTTCAGT+AGG	0.525205	7.2:+62808587	None:intergenic
GTTGGAGGCATTAACAAGAA+GGG	0.555037	7.2:-62808548	MS.gene52525:CDS
ACAAACTTCATAGACCAAAA+GGG	0.556595	7.2:-62808793	MS.gene52525:CDS
AGACCAAAAGGGAAACCAGA+TGG	0.568337	7.2:-62808782	MS.gene52525:CDS
GACCAAAAGGGAAACCAGAT+GGG	0.568542	7.2:-62808781	MS.gene52525:CDS
GCTACATCGGTAGAAATTGA+AGG	0.569826	7.2:+62808467	None:intergenic
TCGTAAATCTCAAACCATGT+TGG	0.585577	7.2:+62808815	None:intergenic
TTGGAGGCATTAACAAGAAG+GGG	0.587641	7.2:-62808547	MS.gene52525:CDS
TCGGTAGAAATTGAAGGCGA+TGG	0.604104	7.2:+62808473	None:intergenic
TTTGAGAAACACTCTCAAAG+TGG	0.627086	7.2:-62808629	MS.gene52525:CDS
AATTGGATAGGGAACCAACA+TGG	0.651250	7.2:-62808829	MS.gene52525:CDS
CAAAAGGGAAACCAGATGGG+TGG	0.669221	7.2:-62808778	MS.gene52525:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTGACAATGATATTTATA+TGG	-	chr7.2:62808717-62808736	MS.gene52525:intron	15.0%
!!	GATATTTATATGGAAATTGT+TGG	-	chr7.2:62808727-62808746	MS.gene52525:intron	20.0%
!	ATTTATATGGAAATTGTTGG+AGG	-	chr7.2:62808730-62808749	MS.gene52525:intron	25.0%
!!!	TAATAGTAACTCTTTTTTGC+AGG	-	chr7.2:62808621-62808640	MS.gene52525:CDS	25.0%
	ACAAACTTCATAGACCAAAA+GGG	-	chr7.2:62808500-62808519	MS.gene52525:CDS	30.0%
	TCATTGTCAATTTCTTCAGT+AGG	+	chr7.2:62808709-62808728	None:intergenic	30.0%
!!	CATACTAACTTGTTGTTTGT+TGG	-	chr7.2:62808423-62808442	MS.gene52525:CDS	30.0%
!!!	TATGTTTTAGAGAGTGGAAT+TGG	-	chr7.2:62808447-62808466	MS.gene52525:CDS	30.0%
	GACAAACTTCATAGACCAAA+AGG	-	chr7.2:62808499-62808518	MS.gene52525:CDS	35.0%
	GTTTCTCAAACGTTTGTGTT+TGG	+	chr7.2:62808654-62808673	None:intergenic	35.0%
	TCGTAAATCTCAAACCATGT+TGG	+	chr7.2:62808481-62808500	None:intergenic	35.0%
	TTAGAGAGTGGAATTGGATA+GGG	-	chr7.2:62808453-62808472	MS.gene52525:CDS	35.0%
	TTTAGAGAGTGGAATTGGAT+AGG	-	chr7.2:62808452-62808471	MS.gene52525:CDS	35.0%
	TTTGAGAAACACTCTCAAAG+TGG	-	chr7.2:62808664-62808683	MS.gene52525:intron	35.0%
!	TTTTTCCGAAGAAGCTACAT+CGG	+	chr7.2:62808842-62808861	None:intergenic	35.0%
!!!	GTTGGTTATGTTTTAGAGAG+TGG	-	chr7.2:62808441-62808460	MS.gene52525:CDS	35.0%
	AATTGGATAGGGAACCAACA+TGG	-	chr7.2:62808464-62808483	MS.gene52525:CDS	40.0%
	GCTACATCGGTAGAAATTGA+AGG	+	chr7.2:62808829-62808848	None:intergenic	40.0%
	GTTTCATTAAACCACCCATC+TGG	+	chr7.2:62808529-62808548	None:intergenic	40.0%
	TTCGGAAAAAGTTGCTGCTA+TGG	-	chr7.2:62808852-62808871	MS.gene52525:intron	40.0%
	TTCTACCGATGTAGCTTCTT+CGG	-	chr7.2:62808834-62808853	MS.gene52525:CDS	40.0%
!	AACAAGAAGGGGCACATATT+TGG	-	chr7.2:62808757-62808776	MS.gene52525:CDS	40.0%
!	AAGGGGCACATATTTGGATT+AGG	-	chr7.2:62808763-62808782	MS.gene52525:CDS	40.0%
!!	GTTGGAGGCATTAACAAGAA+GGG	-	chr7.2:62808745-62808764	MS.gene52525:intron	40.0%
!!	TGTTGGAGGCATTAACAAGA+AGG	-	chr7.2:62808744-62808763	MS.gene52525:intron	40.0%
!!	TTGGAGGCATTAACAAGAAG+GGG	-	chr7.2:62808746-62808765	MS.gene52525:intron	40.0%
	AGACCAAAAGGGAAACCAGA+TGG	-	chr7.2:62808511-62808530	MS.gene52525:CDS	45.0%
	GACCAAAAGGGAAACCAGAT+GGG	-	chr7.2:62808512-62808531	MS.gene52525:CDS	45.0%
	GGAAAAAGTTGCTGCTATGG+AGG	-	chr7.2:62808855-62808874	MS.gene52525:intron	45.0%
	TCGGTAGAAATTGAAGGCGA+TGG	+	chr7.2:62808823-62808842	None:intergenic	45.0%
	CAAAAGGGAAACCAGATGGG+TGG	-	chr7.2:62808515-62808534	MS.gene52525:CDS	50.0%
!!	CACCCATCTGGTTTCCCTTT+TGG	+	chr7.2:62808517-62808536	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	62808379	62808936	62808379	ID=MS.gene52525
chr7.2	mRNA	62808379	62808936	62808379	ID=MS.gene52525.t1;Parent=MS.gene52525
chr7.2	exon	62808934	62808936	62808934	ID=MS.gene52525.t1.exon1;Parent=MS.gene52525.t1
chr7.2	CDS	62808934	62808936	62808934	ID=cds.MS.gene52525.t1;Parent=MS.gene52525.t1
chr7.2	exon	62808751	62808858	62808751	ID=MS.gene52525.t1.exon2;Parent=MS.gene52525.t1
chr7.2	CDS	62808751	62808858	62808751	ID=cds.MS.gene52525.t1;Parent=MS.gene52525.t1
chr7.2	exon	62808379	62808672	62808379	ID=MS.gene52525.t1.exon3;Parent=MS.gene52525.t1
chr7.2	CDS	62808379	62808672	62808379	ID=cds.MS.gene52525.t1;Parent=MS.gene52525.t1

Gene Sequence

>MS.gene52525

ATGGTTAGTGATTCTTCTTGTTTAAATTACAATGTAAATATGTTCATACTAACTTGTTGTTTGTTGGTTATGTTTTAGAGAGTGGAATTGGATAGGGAACCAACATGGTTTGAGATTTACGACAAACTTCATAGACCAAAAGGGAAACCAGATGGGTGGTTTAATGAAACACAAGCTAAGATAGCTGTATGTATACACAATTTTTATTTTATTTTATCTTTCATATATCTAAATTATATTACTAATAGTAACTCTTTTTTGCAGGAAATGTACCAAACACAAACGTTTGAGAAACACTCTCAAAGTGGTGAGATTAGTAGTCAACAACCTACTGAAGAAATTGACAATGATATTTATATGGAAATTGTTGGAGGCATTAACAAGAAGGGGCACATATTTGGATTAGGATCTCAAGCTGTTGTTGTCAAAGAATCTTTGAGACCATCGCCTTCAATTTCTACCGATGTAGCTTCTTCGGAAAAAGTTGCTGCTATGGAGGCTAAGATTGAAGCATTAACTTCTGAACTTGAAAAAAAAATCTTGAACAAGAAACGCTAA

Protein sequence

>MS.gene52525.t1

MRVELDREPTWFEIYDKLHRPKGKPDGWFNETQAKIAEMYQTQTFEKHSQSGEISSQQPTEEIDNDIYMEIVGGINKKGHIFGLGSQAVVVKESLRPSPSISTDVASSEKVAAMEAKIEALTSELEKKILNKKR