AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31283


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31283.t1 MTR_2g094570 100.000 71 0 0 1 71 685 755 1.15e-42 146
MS.gene31283.t1 MTR_2g094570 100.000 71 0 0 1 71 629 699 1.20e-42 145
MS.gene31283.t1 MTR_4g058930 75.714 70 17 0 1 70 628 697 5.43e-30 110
MS.gene31283.t1 MTR_4g058930 79.545 44 9 0 1 44 628 671 1.38e-17 75.1
MS.gene31283.t1 MTR_1g064430 75.676 37 9 0 2 38 651 687 1.12e-13 63.9
MS.gene31283.t1 MTR_1g064430 75.676 37 9 0 2 38 664 700 1.16e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31283.t1 AT2G28350 60.000 70 28 0 1 70 623 692 3.61e-24 94.0
MS.gene31283.t1 AT4G30080 68.293 41 13 0 1 41 626 666 2.42e-13 63.2
MS.gene31283.t1 AT2G28350 57.778 45 19 0 1 45 623 667 8.19e-13 61.6

Find 10 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACAATTCTGATGGATTC+AGG 0.309644 2.4:+7918188 MS.gene31283:CDS
GTACTCTATCGCGATGCAAC+AGG 0.393430 2.4:+7917996 MS.gene31283:CDS
ATTTGCAGGGTTTGTATCAC+AGG 0.434573 2.4:+7918342 MS.gene31283:intron
CAGGCAGCAAAGATACTAGA+AGG 0.487275 2.4:+7918207 MS.gene31283:CDS
ACAGGTGCTGTTAAACAAAC+TGG 0.490968 2.4:+7918014 MS.gene31283:CDS
TCTTAAACAAAGATGCTCAT+AGG 0.516080 2.4:-7918411 None:intergenic
AAACTGGTGAAGAACCCTTC+AGG 0.541832 2.4:+7918030 MS.gene31283:CDS
TTAGATGCATCAAATAAGAC+AGG 0.558939 2.4:+7918387 MS.gene31283:CDS
GGGTTTGTATCACAGGAACT+AGG 0.573351 2.4:+7918349 MS.gene31283:intron
GGAACTAGGAATGCTGAACG+TGG 0.666679 2.4:+7918363 MS.gene31283:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATATATTCATATTCATA+TGG - chr2.4:7918081-7918100 None:intergenic 15.0%
!! TTGTTGAATTATTATTTGCA+GGG + chr2.4:7918329-7918348 MS.gene31283:intron 20.0%
!! TTTGTTGAATTATTATTTGC+AGG + chr2.4:7918328-7918347 MS.gene31283:intron 20.0%
! AAAAAGATTGACAATTCTGA+TGG + chr2.4:7918181-7918200 MS.gene31283:CDS 25.0%
! AAACAAGAAAAATACCTGAA+GGG - chr2.4:7918047-7918066 None:intergenic 25.0%
!! ACGTAAACTTTTTACATTGT+CGG + chr2.4:7918125-7918144 MS.gene31283:intron 25.0%
GAAACAAGAAAAATACCTGA+AGG - chr2.4:7918048-7918067 None:intergenic 30.0%
TTAGATGCATCAAATAAGAC+AGG + chr2.4:7918387-7918406 MS.gene31283:CDS 30.0%
AGGTCTTTAGATGTGTATGA+AGG + chr2.4:7918293-7918312 MS.gene31283:intron 35.0%
GTGTCTTTAGATGTGTATGA+AGG + chr2.4:7918273-7918292 MS.gene31283:intron 35.0%
!! TTGACAATTCTGATGGATTC+AGG + chr2.4:7918188-7918207 MS.gene31283:CDS 35.0%
ATTTGCAGGGTTTGTATCAC+AGG + chr2.4:7918342-7918361 MS.gene31283:intron 40.0%
!! ACAGGTGCTGTTAAACAAAC+TGG + chr2.4:7918014-7918033 MS.gene31283:CDS 40.0%
AAACTGGTGAAGAACCCTTC+AGG + chr2.4:7918030-7918049 MS.gene31283:CDS 45.0%
CAGGCAGCAAAGATACTAGA+AGG + chr2.4:7918207-7918226 MS.gene31283:CDS 45.0%
GGGTTTGTATCACAGGAACT+AGG + chr2.4:7918349-7918368 MS.gene31283:intron 45.0%
GGAACTAGGAATGCTGAACG+TGG + chr2.4:7918363-7918382 MS.gene31283:CDS 50.0%
GTACTCTATCGCGATGCAAC+AGG + chr2.4:7917996-7918015 MS.gene31283:CDS 50.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7917984 7918431 7917984 ID=MS.gene31283
chr2.4 mRNA 7917984 7918431 7917984 ID=MS.gene31283.t1;Parent=MS.gene31283
chr2.4 exon 7917984 7918051 7917984 ID=MS.gene31283.t1.exon1;Parent=MS.gene31283.t1
chr2.4 CDS 7917984 7918051 7917984 ID=cds.MS.gene31283.t1;Parent=MS.gene31283.t1
chr2.4 exon 7918163 7918228 7918163 ID=MS.gene31283.t1.exon2;Parent=MS.gene31283.t1
chr2.4 CDS 7918163 7918228 7918163 ID=cds.MS.gene31283.t1;Parent=MS.gene31283.t1
chr2.4 exon 7918350 7918431 7918350 ID=MS.gene31283.t1.exon3;Parent=MS.gene31283.t1
chr2.4 CDS 7918350 7918431 7918350 ID=cds.MS.gene31283.t1;Parent=MS.gene31283.t1
Gene Sequence

>MS.gene31283

ATGTTGAGTCGTGTACTCTATCGCGATGCAACAGGTGCTGTTAAACAAACTGGTGAAGAACCCTTCAGGTATTTTTCTTGTTTCATCTTTTTATCCATATGAATATGAATATATTTCGAGTTTAATTTATATGCATCGTCGACGTAAACTTTTTACATTGTCGGTTAATTACATTGTAGTGACTTCATGAAGACAGCAAAAAGATTGACAATTCTGATGGATTCAGGCAGCAAAGATACTAGAAGGTGTGATTAAAAAAAAAAACATTGTTATATTTAATTATTAGTTAGTGTCTTTAGATGTGTATGAAGGTCTTTAGATGTGTATGAAGGTACTAAACATTTTTTGTTGAATTATTATTTGCAGGGTTTGTATCACAGGAACTAGGAATGCTGAACGTGGATTAGATGCATCAAATAAGACAGGACCTATGAGCATCTTTGTTTAA

Protein sequence

>MS.gene31283.t1

MLSRVLYRDATGAVKQTGEEPFSDFMKTAKRLTILMDSGSKDTRRVCITGTRNAERGLDASNKTGPMSIFV