Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06269.t1 | AFK35673.1 | 75.4 | 61 | 3 | 1 | 124 | 172 | 32 | 92 | 1.20E-13 | 87 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06269.t1 | Q43207 | 67.2 | 61 | 8 | 1 | 124 | 172 | 34 | 94 | 8.6e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06269.t1 | G7LF61 | 75.4 | 61 | 3 | 1 | 124 | 172 | 32 | 92 | 8.6e-14 | 87.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene06269 | 0.820877 | 5.44E-53 | -1.69E-46 |
| MS.gene049203 | MS.gene06269 | 0.801889 | 7.28E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene06269 | 0.881043 | 3.33E-70 | -1.69E-46 |
| MS.gene049262 | MS.gene06269 | 0.806155 | 9.44E-50 | -1.69E-46 |
| MS.gene049272 | MS.gene06269 | 0.828355 | 9.41E-55 | -1.69E-46 |
| MS.gene049343 | MS.gene06269 | 0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene049404 | MS.gene06269 | 0.825722 | 4.01E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene06269 | 0.837184 | 6.04E-57 | -1.69E-46 |
| MS.gene049452 | MS.gene06269 | 0.804912 | 1.72E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene06269 | 0.804613 | 1.99E-49 | -1.69E-46 |
| MS.gene049587 | MS.gene06269 | 0.807876 | 4.08E-50 | -1.69E-46 |
| MS.gene049666 | MS.gene06269 | 0.829317 | 5.51E-55 | -1.69E-46 |
| MS.gene049669 | MS.gene06269 | 0.826308 | 2.91E-54 | -1.69E-46 |
| MS.gene049850 | MS.gene06269 | 0.809495 | 1.84E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene06269 | 0.809481 | 1.85E-50 | -1.69E-46 |
| MS.gene050021 | MS.gene06269 | 0.809642 | 1.71E-50 | -1.69E-46 |
| MS.gene050026 | MS.gene06269 | 0.863405 | 2.55E-64 | -1.69E-46 |
| MS.gene050029 | MS.gene06269 | 0.824583 | 7.46E-54 | -1.69E-46 |
| MS.gene050073 | MS.gene06269 | 0.817765 | 2.78E-52 | -1.69E-46 |
| MS.gene050156 | MS.gene06269 | 0.810583 | 1.07E-50 | -1.69E-46 |
| MS.gene050201 | MS.gene06269 | 0.813172 | 2.93E-51 | -1.69E-46 |
| MS.gene050273 | MS.gene06269 | 0.82297 | 1.78E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene06269 | 0.853059 | 3.07E-61 | -1.69E-46 |
| MS.gene050442 | MS.gene06269 | 0.828489 | 8.74E-55 | -1.69E-46 |
| MS.gene050559 | MS.gene06269 | 0.811646 | 6.31E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene06269 | 0.811524 | 6.71E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene06269 | 0.865171 | 7.16E-65 | -1.69E-46 |
| MS.gene050750 | MS.gene06269 | 0.830982 | 2.16E-55 | -1.69E-46 |
| MS.gene050799 | MS.gene06269 | 0.815047 | 1.13E-51 | -1.69E-46 |
| MS.gene050844 | MS.gene06269 | 0.803378 | 3.59E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene06269 | 0.834435 | 3.00E-56 | -1.69E-46 |
| MS.gene051181 | MS.gene06269 | 0.838543 | 2.70E-57 | -1.69E-46 |
| MS.gene051198 | MS.gene06269 | 0.843379 | 1.45E-58 | -1.69E-46 |
| MS.gene051247 | MS.gene06269 | 0.801863 | 7.37E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene06269 | 0.829883 | 4.01E-55 | -1.69E-46 |
| MS.gene051332 | MS.gene06269 | 0.8132 | 2.89E-51 | -1.69E-46 |
| MS.gene051543 | MS.gene06269 | 0.801725 | 7.86E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene06269 | 0.805768 | 1.14E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene06269 | 0.842985 | 1.85E-58 | -1.69E-46 |
| MS.gene051744 | MS.gene06269 | 0.841998 | 3.38E-58 | -1.69E-46 |
| MS.gene051800 | MS.gene06269 | 0.801902 | 7.23E-49 | -1.69E-46 |
| MS.gene051957 | MS.gene06269 | 0.814832 | 1.26E-51 | -1.69E-46 |
| MS.gene052037 | MS.gene06269 | 0.809677 | 1.68E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene06269 | 0.826409 | 2.76E-54 | -1.69E-46 |
| MS.gene052208 | MS.gene06269 | 0.833458 | 5.27E-56 | -1.69E-46 |
| MS.gene052333 | MS.gene06269 | 0.839787 | 1.29E-57 | -1.69E-46 |
| MS.gene052488 | MS.gene06269 | 0.801337 | 9.44E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene06269 | 0.872784 | 2.44E-67 | -1.69E-46 |
| MS.gene052582 | MS.gene06269 | 0.87493 | 4.59E-68 | -1.69E-46 |
| MS.gene052584 | MS.gene06269 | 0.882605 | 9.06E-71 | -1.69E-46 |
| MS.gene052586 | MS.gene06269 | 0.822994 | 1.76E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene06269 | 0.803329 | 3.67E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene06269 | 0.821868 | 3.21E-53 | -1.69E-46 |
| MS.gene052829 | MS.gene06269 | 0.812792 | 3.55E-51 | -1.69E-46 |
| MS.gene052843 | MS.gene06269 | 0.823191 | 1.58E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene06269 | 0.817656 | 2.94E-52 | -1.69E-46 |
| MS.gene052866 | MS.gene06269 | 0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene052867 | MS.gene06269 | 0.846692 | 1.85E-59 | -1.69E-46 |
| MS.gene053006 | MS.gene06269 | 0.837513 | 4.97E-57 | -1.69E-46 |
| MS.gene05308 | MS.gene06269 | 0.814545 | 1.46E-51 | -1.69E-46 |
| MS.gene053145 | MS.gene06269 | 0.824807 | 6.61E-54 | -1.69E-46 |
| MS.gene053166 | MS.gene06269 | 0.83666 | 8.21E-57 | -1.69E-46 |
| MS.gene053201 | MS.gene06269 | 0.810508 | 1.11E-50 | -1.69E-46 |
| MS.gene053202 | MS.gene06269 | 0.811466 | 6.90E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene06269 | 0.838232 | 3.25E-57 | -1.69E-46 |
| MS.gene053296 | MS.gene06269 | 0.800858 | 1.18E-48 | -1.69E-46 |
| MS.gene05329 | MS.gene06269 | 0.811326 | 7.41E-51 | -1.69E-46 |
| MS.gene05365 | MS.gene06269 | 0.824418 | 8.16E-54 | -1.69E-46 |
| MS.gene053883 | MS.gene06269 | 0.818364 | 2.04E-52 | -1.69E-46 |
| MS.gene053900 | MS.gene06269 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene053953 | MS.gene06269 | 0.813077 | 3.07E-51 | -1.69E-46 |
| MS.gene053966 | MS.gene06269 | 0.82358 | 1.28E-53 | -1.69E-46 |
| MS.gene053967 | MS.gene06269 | 0.817011 | 4.11E-52 | -1.69E-46 |
| MS.gene053974 | MS.gene06269 | 0.848762 | 4.98E-60 | -1.69E-46 |
| MS.gene053998 | MS.gene06269 | 0.830547 | 2.76E-55 | -1.69E-46 |
| MS.gene05414 | MS.gene06269 | 0.853413 | 2.43E-61 | -1.69E-46 |
| MS.gene05415 | MS.gene06269 | 0.867918 | 9.60E-66 | -1.69E-46 |
| MS.gene054236 | MS.gene06269 | 0.846391 | 2.24E-59 | -1.69E-46 |
| MS.gene05423 | MS.gene06269 | 0.822051 | 2.91E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene06269 | -0.849755 | 2.64E-60 | -1.69E-46 |
| MS.gene05435 | MS.gene06269 | 0.803438 | 3.49E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene06269 | 0.827165 | 1.82E-54 | -1.69E-46 |
| MS.gene054467 | MS.gene06269 | 0.821364 | 4.20E-53 | -1.69E-46 |
| MS.gene054468 | MS.gene06269 | 0.811758 | 5.97E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene06269 | 0.83464 | 2.67E-56 | -1.69E-46 |
| MS.gene054651 | MS.gene06269 | 0.875443 | 3.07E-68 | -1.69E-46 |
| MS.gene054657 | MS.gene06269 | 0.802627 | 5.13E-49 | -1.69E-46 |
| MS.gene054661 | MS.gene06269 | 0.808269 | 3.36E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene06269 | 0.83602 | 1.20E-56 | -1.69E-46 |
| MS.gene054991 | MS.gene06269 | 0.826584 | 2.50E-54 | -1.69E-46 |
| MS.gene055056 | MS.gene06269 | 0.809083 | 2.25E-50 | -1.69E-46 |
| MS.gene055058 | MS.gene06269 | 0.803858 | 2.85E-49 | -1.69E-46 |
| MS.gene055166 | MS.gene06269 | 0.840866 | 6.72E-58 | -1.69E-46 |
| MS.gene055227 | MS.gene06269 | 0.813779 | 2.15E-51 | -1.69E-46 |
| MS.gene055228 | MS.gene06269 | 0.856014 | 4.28E-62 | -1.69E-46 |
| MS.gene05531 | MS.gene06269 | 0.802797 | 4.73E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene06269 | 0.829785 | 4.24E-55 | -1.69E-46 |
| MS.gene055586 | MS.gene06269 | 0.851163 | 1.06E-60 | -1.69E-46 |
| MS.gene055589 | MS.gene06269 | 0.832174 | 1.10E-55 | -1.69E-46 |
| MS.gene055694 | MS.gene06269 | 0.815415 | 9.35E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene06269 | 0.837838 | 4.10E-57 | -1.69E-46 |
| MS.gene055818 | MS.gene06269 | 0.864756 | 9.67E-65 | -1.69E-46 |
| MS.gene055841 | MS.gene06269 | 0.801683 | 8.02E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene06269 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene06269 | 0.847637 | 1.02E-59 | -1.69E-46 |
| MS.gene056162 | MS.gene06269 | 0.842838 | 2.02E-58 | -1.69E-46 |
| MS.gene056173 | MS.gene06269 | 0.815416 | 9.35E-52 | -1.69E-46 |
| MS.gene056174 | MS.gene06269 | 0.859973 | 2.86E-63 | -1.69E-46 |
| MS.gene056252 | MS.gene06269 | 0.8155 | 8.95E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene06269 | 0.832633 | 8.46E-56 | -1.69E-46 |
| MS.gene056373 | MS.gene06269 | 0.812184 | 4.82E-51 | -1.69E-46 |
| MS.gene056498 | MS.gene06269 | 0.842119 | 3.14E-58 | -1.69E-46 |
| MS.gene056506 | MS.gene06269 | 0.833537 | 5.04E-56 | -1.69E-46 |
| MS.gene056595 | MS.gene06269 | 0.821658 | 3.59E-53 | -1.69E-46 |
| MS.gene05659 | MS.gene06269 | 0.806013 | 1.01E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene06269 | 0.820216 | 7.71E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene06269 | 0.825571 | 4.36E-54 | -1.69E-46 |
| MS.gene056873 | MS.gene06269 | 0.814093 | 1.84E-51 | -1.69E-46 |
| MS.gene05690 | MS.gene06269 | 0.825673 | 4.12E-54 | -1.69E-46 |
| MS.gene057021 | MS.gene06269 | 0.811453 | 6.95E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene06269 | 0.824233 | 9.02E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene06269 | 0.828988 | 6.62E-55 | -1.69E-46 |
| MS.gene057060 | MS.gene06269 | 0.829737 | 4.35E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene06269 | 0.835358 | 1.76E-56 | -1.69E-46 |
| MS.gene057314 | MS.gene06269 | 0.871757 | 5.36E-67 | -1.69E-46 |
| MS.gene057414 | MS.gene06269 | 0.822296 | 2.56E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene06269 | 0.839207 | 1.82E-57 | -1.69E-46 |
| MS.gene057598 | MS.gene06269 | 0.84752 | 1.10E-59 | -1.69E-46 |
| MS.gene057693 | MS.gene06269 | 0.812084 | 5.06E-51 | -1.69E-46 |
| MS.gene057855 | MS.gene06269 | 0.802965 | 4.37E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene06269 | 0.83121 | 1.90E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene06269 | 0.816815 | 4.55E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene06269 | 0.824381 | 8.32E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene06269 | 0.830157 | 3.44E-55 | -1.69E-46 |
| MS.gene058037 | MS.gene06269 | 0.863187 | 2.98E-64 | -1.69E-46 |
| MS.gene058294 | MS.gene06269 | 0.846292 | 2.38E-59 | -1.69E-46 |
| MS.gene058502 | MS.gene06269 | 0.806171 | 9.36E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene06269 | 0.813838 | 2.09E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene06269 | 0.814674 | 1.37E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene06269 | 0.846324 | 2.33E-59 | -1.69E-46 |
| MS.gene058783 | MS.gene06269 | 0.81844 | 1.96E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene06269 | 0.834822 | 2.40E-56 | -1.69E-46 |
| MS.gene058979 | MS.gene06269 | 0.87104 | 9.25E-67 | -1.69E-46 |
| MS.gene059015 | MS.gene06269 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene05925 | MS.gene06269 | 0.809272 | 2.05E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene06269 | 0.842592 | 2.35E-58 | -1.69E-46 |
| MS.gene059276 | MS.gene06269 | 0.826537 | 2.57E-54 | -1.69E-46 |
| MS.gene059382 | MS.gene06269 | 0.800962 | 1.13E-48 | -1.69E-46 |
| MS.gene059458 | MS.gene06269 | 0.815651 | 8.29E-52 | -1.69E-46 |
| MS.gene059459 | MS.gene06269 | 0.830862 | 2.31E-55 | -1.69E-46 |
| MS.gene059460 | MS.gene06269 | 0.807738 | 4.37E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene06269 | 0.847896 | 8.65E-60 | -1.69E-46 |
| MS.gene059550 | MS.gene06269 | 0.819684 | 1.02E-52 | -1.69E-46 |
| MS.gene059557 | MS.gene06269 | 0.802926 | 4.45E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene06269 | 0.840813 | 6.93E-58 | -1.69E-46 |
| MS.gene059625 | MS.gene06269 | 0.841447 | 4.73E-58 | -1.69E-46 |
| MS.gene05979 | MS.gene06269 | 0.808221 | 3.44E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene06269 | 0.801458 | 8.92E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene06269 | 0.849959 | 2.31E-60 | -1.69E-46 |
| MS.gene060137 | MS.gene06269 | -0.804118 | 2.52E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene06269 | 0.801791 | 7.62E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene06269 | 0.811503 | 6.78E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene06269 | 0.827954 | 1.18E-54 | -1.69E-46 |
| MS.gene060521 | MS.gene06269 | 0.840993 | 6.22E-58 | -1.69E-46 |
| MS.gene06052 | MS.gene06269 | 0.815894 | 7.32E-52 | -1.69E-46 |
| MS.gene060553 | MS.gene06269 | 0.828702 | 7.76E-55 | -1.69E-46 |
| MS.gene060572 | MS.gene06269 | 0.825795 | 3.86E-54 | -1.69E-46 |
| MS.gene060634 | MS.gene06269 | -0.838085 | 3.54E-57 | -1.69E-46 |
| MS.gene060781 | MS.gene06269 | 0.812276 | 4.60E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene06269 | 0.814954 | 1.18E-51 | -1.69E-46 |
| MS.gene060905 | MS.gene06269 | 0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene060918 | MS.gene06269 | 0.838395 | 2.95E-57 | -1.69E-46 |
| MS.gene060919 | MS.gene06269 | 0.820164 | 7.93E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene06269 | -0.818303 | 2.10E-52 | -1.69E-46 |
| MS.gene061091 | MS.gene06269 | 0.807722 | 4.40E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06269.t1 | MTR_8g432580 | 86.667 | 75 | 10 | 0 | 18 | 92 | 52 | 126 | 1.32e-23 | 92.8 |
| MS.gene06269.t1 | MTR_4g074930 | 87.838 | 74 | 8 | 1 | 14 | 87 | 28 | 100 | 6.12e-23 | 97.1 |
| MS.gene06269.t1 | MTR_4g074930 | 87.838 | 74 | 8 | 1 | 14 | 87 | 28 | 100 | 6.52e-23 | 96.7 |
| MS.gene06269.t1 | MTR_4g074930 | 87.838 | 74 | 8 | 1 | 14 | 87 | 28 | 100 | 7.16e-23 | 96.7 |
| MS.gene06269.t1 | MTR_4g074930 | 87.838 | 74 | 8 | 1 | 14 | 87 | 28 | 100 | 7.58e-23 | 96.7 |
| MS.gene06269.t1 | MTR_8g080980 | 69.492 | 59 | 6 | 1 | 126 | 172 | 32 | 90 | 3.97e-17 | 80.1 |
| MS.gene06269.t1 | MTR_8g012340 | 90.909 | 33 | 3 | 0 | 140 | 172 | 60 | 92 | 2.71e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06269.t1 | AT5G48570 | 67.797 | 59 | 7 | 1 | 127 | 173 | 42 | 100 | 2.14e-16 | 77.8 |
| MS.gene06269.t1 | AT3G25230 | 63.636 | 66 | 11 | 2 | 119 | 172 | 27 | 91 | 1.90e-15 | 75.1 |
| MS.gene06269.t1 | AT3G25230 | 63.636 | 66 | 11 | 2 | 119 | 172 | 27 | 91 | 2.00e-15 | 75.1 |
Find 55 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGTGTAGTTCATTATAC+TGG | 0.313269 | 3.1:+78386273 | MS.gene06269:intron |
| CCTCTGAAGTGGTCTCTGGC+TGG | 0.335134 | 3.1:-78385569 | None:intergenic |
| CAAGAAGGAGGAAAGTAGTT+TGG | 0.335928 | 3.1:+78385331 | MS.gene06269:CDS |
| TTAGTTGGGGTCTTCTTTGA+AGG | 0.356776 | 3.1:-78385525 | None:intergenic |
| ACTACTTTCAATTTCACTCT+TGG | 0.361943 | 3.1:+78386342 | MS.gene06269:CDS |
| CTCTGAAGTGGTCTCTGGCT+GGG | 0.379329 | 3.1:-78385568 | None:intergenic |
| AGTTTGATTCTAGCAGAGAT+AGG | 0.388381 | 3.1:+78386316 | MS.gene06269:CDS |
| TATACTGGAACGTTGCTTGA+TGG | 0.393092 | 3.1:+78386288 | MS.gene06269:CDS |
| TCCCACATCGTACTATTATC+AGG | 0.413728 | 3.1:+78386917 | MS.gene06269:CDS |
| GATTCCTCTGAAGTGGTCTC+TGG | 0.415465 | 3.1:-78385573 | None:intergenic |
| CACTTCTTCCTCATCGGAAG+AGG | 0.423585 | 3.1:-78385613 | None:intergenic |
| CTTCAAGAATAATAATAGAT+AGG | 0.425201 | 3.1:-78386540 | None:intergenic |
| CGCCAAGCAAAAGGCGTTGA+AGG | 0.441849 | 3.1:+78385304 | MS.gene06269:CDS |
| GGGGTCTTCTTTGAAGGAGC+AGG | 0.444374 | 3.1:-78385519 | None:intergenic |
| AAGAAGAAGCTTCTCAAAGA+AGG | 0.467998 | 3.1:+78386154 | MS.gene06269:CDS |
| GTTTGATTCTAGCAGAGATA+GGG | 0.475863 | 3.1:+78386317 | MS.gene06269:CDS |
| TAGGCAGATGGAAGGTGATA+AGG | 0.476180 | 3.1:-78386521 | None:intergenic |
| AGAGGTTGCCGCCAAGCAAA+AGG | 0.479123 | 3.1:+78385295 | MS.gene06269:CDS |
| ATCTATTATTATTCTTGAAG+AGG | 0.490271 | 3.1:+78386543 | MS.gene06269:CDS |
| CTCTGAGGAATCTGAGGATG+AGG | 0.511350 | 3.1:+78385361 | MS.gene06269:CDS |
| GAAGGAGGAAAGTAGTTTGG+AGG | 0.514144 | 3.1:+78385334 | MS.gene06269:CDS |
| AATTGGATTATCGCCGGCAC+AGG | 0.517693 | 3.1:-78386088 | None:intergenic |
| AATGAAATTAAAAGCATGGC+AGG | 0.519602 | 3.1:+78386885 | MS.gene06269:CDS |
| GGCGTTGAAGGTAGTCAAGA+AGG | 0.528027 | 3.1:+78385316 | MS.gene06269:CDS |
| GTGGGTACTAGAGAATTCAT+AGG | 0.534544 | 3.1:+78386943 | MS.gene06269:CDS |
| TTCAATTTCACTCTTGGACA+AGG | 0.538952 | 3.1:+78386348 | MS.gene06269:CDS |
| CAGTGAGTGCAAAGAAGCAA+GGG | 0.541082 | 3.1:+78385267 | MS.gene06269:CDS |
| CTTTGATATAGGCAAGAGGC+AGG | 0.543584 | 3.1:+78385226 | MS.gene06269:CDS |
| GTCAAATGAAATTAAAAGCA+TGG | 0.545516 | 3.1:+78386881 | MS.gene06269:CDS |
| GCAGGCTGAGGAAGAAGTCA+AGG | 0.547712 | 3.1:+78385244 | MS.gene06269:CDS |
| AGAGTCAGATTCCTCTGAAG+TGG | 0.548311 | 3.1:-78385580 | None:intergenic |
| GCAGTGAGTGCAAAGAAGCA+AGG | 0.558038 | 3.1:+78385266 | MS.gene06269:CDS |
| AATAATAATAGATAGGCAGA+TGG | 0.568838 | 3.1:-78386533 | None:intergenic |
| CTTGAGAATTGGATTATCGC+CGG | 0.576084 | 3.1:-78386094 | None:intergenic |
| CATATGTAGACACTGTCATA+GGG | 0.583382 | 3.1:-78386974 | None:intergenic |
| GGAATCCTCTGAGGAATCTG+AGG | 0.584932 | 3.1:+78385355 | MS.gene06269:CDS |
| GACTTCGACTTCGCCTGTGC+CGG | 0.588185 | 3.1:+78386075 | MS.gene06269:CDS |
| TATAGGCAAGAGGCAGGCTG+AGG | 0.588543 | 3.1:+78385232 | MS.gene06269:CDS |
| TTTGATTCTAGCAGAGATAG+GGG | 0.593989 | 3.1:+78386318 | MS.gene06269:CDS |
| TCGTACTATTATCAGGCAAG+TGG | 0.597519 | 3.1:+78386924 | MS.gene06269:CDS |
| AAAGAAGCAAGGGGTAGAAG+AGG | 0.598623 | 3.1:+78385277 | MS.gene06269:CDS |
| AAGCTTCTCAAAGAAGGTGA+AGG | 0.613170 | 3.1:+78386160 | MS.gene06269:CDS |
| CGGCGATAATCCAATTCTCA+AGG | 0.616014 | 3.1:+78386095 | MS.gene06269:CDS |
| TGCCTGATAATAGTACGATG+TGG | 0.623695 | 3.1:-78386919 | None:intergenic |
| CCAGCCAGAGACCACTTCAG+AGG | 0.625597 | 3.1:+78385569 | MS.gene06269:CDS |
| ATAATAGATAGGCAGATGGA+AGG | 0.627317 | 3.1:-78386529 | None:intergenic |
| TTCACTTTGATATAGGCAAG+AGG | 0.629817 | 3.1:+78385222 | MS.gene06269:CDS |
| CGTACTATTATCAGGCAAGT+GGG | 0.636740 | 3.1:+78386925 | MS.gene06269:CDS |
| GCCTGATAATAGTACGATGT+GGG | 0.647015 | 3.1:-78386918 | None:intergenic |
| TGACAGCTCCTCTTCCGATG+AGG | 0.667005 | 3.1:+78385605 | MS.gene06269:CDS |
| AGTGAGTGCAAAGAAGCAAG+GGG | 0.679652 | 3.1:+78385268 | MS.gene06269:CDS |
| GTTGAAGGTAGTCAAGAAGG+AGG | 0.683060 | 3.1:+78385319 | MS.gene06269:CDS |
| CTCATCCTCAGATTCCTCAG+AGG | 0.686828 | 3.1:-78385360 | None:intergenic |
| GATAATCCAATTCTCAAGGT+CGG | 0.697498 | 3.1:+78386099 | MS.gene06269:CDS |
| TAGTTTGGAGGAATCCTCTG+AGG | 0.723183 | 3.1:+78385346 | MS.gene06269:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATTTTTGTTATTTTAAT+TGG | + | chr3.1:78386395-78386414 | MS.gene06269:intron | 10.0% |
| !! | AAATCAAACATAGAAATTAA+CGG | - | chr3.1:78386423-78386442 | None:intergenic | 15.0% |
| !!! | ATGTTTAAGATATAAATCAA+AGG | - | chr3.1:78385692-78385711 | None:intergenic | 15.0% |
| !!! | GTTTTAAAAAATAGAACAAT+AGG | - | chr3.1:78385783-78385802 | None:intergenic | 15.0% |
| !!! | TTAATTTCTATGTTTGATTT+AGG | + | chr3.1:78386423-78386442 | MS.gene06269:intron | 15.0% |
| !! | ATCTATTATTATTCTTGAAG+AGG | + | chr3.1:78386543-78386562 | MS.gene06269:CDS | 20.0% |
| !! | CTTCAAGAATAATAATAGAT+AGG | - | chr3.1:78386543-78386562 | None:intergenic | 20.0% |
| !!! | GTGTAATTATTTTTTTGTTG+CGG | + | chr3.1:78385984-78386003 | MS.gene06269:intron | 20.0% |
| !!! | TCTATAATTCACTTTTTAGA+AGG | + | chr3.1:78385806-78385825 | MS.gene06269:intron | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | - | chr3.1:78386785-78386804 | None:intergenic | 20.0% |
| !!! | TTCTAAAAAGTGAATTATAG+AGG | - | chr3.1:78385807-78385826 | None:intergenic | 20.0% |
| ! | AATAATAATAGATAGGCAGA+TGG | - | chr3.1:78386536-78386555 | None:intergenic | 25.0% |
| ! | GATTTCAAACAATAAAAAGC+AGG | + | chr3.1:78386633-78386652 | MS.gene06269:intron | 25.0% |
| ! | GTCAAATGAAATTAAAAGCA+TGG | + | chr3.1:78386881-78386900 | MS.gene06269:CDS | 25.0% |
| ! | TTATATCTTAAACACTGTGT+TGG | + | chr3.1:78385754-78385773 | MS.gene06269:intron | 25.0% |
| ! | TTATGTGTAGTTCATTATAC+TGG | + | chr3.1:78386273-78386292 | MS.gene06269:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | - | chr3.1:78386726-78386745 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | - | chr3.1:78386725-78386744 | None:intergenic | 25.0% |
| !! | TATGTCATGTTAATTTTACG+TGG | + | chr3.1:78385472-78385491 | MS.gene06269:intron | 25.0% |
| !! | TGTTTGATTTAGGTTATGTT+TGG | + | chr3.1:78386433-78386452 | MS.gene06269:intron | 25.0% |
| !!! | CGGTTTATATTTTTTTGTTG+CGG | + | chr3.1:78386004-78386023 | MS.gene06269:intron | 25.0% |
| !!! | GAGGTAAATTTTTAAAAGTC+AGG | + | chr3.1:78386562-78386581 | MS.gene06269:intron | 25.0% |
| AATCAACGAAGAAAGAACAT+AGG | - | chr3.1:78386212-78386231 | None:intergenic | 30.0% | |
| ACTACTTTCAATTTCACTCT+TGG | + | chr3.1:78386342-78386361 | MS.gene06269:CDS | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | + | chr3.1:78386722-78386741 | MS.gene06269:intron | 30.0% | |
| GTAAAATTTCGATATTCCTG+TGG | + | chr3.1:78386035-78386054 | MS.gene06269:intron | 30.0% | |
| TAGTTATCAGCAAATGAGAT+AGG | + | chr3.1:78386697-78386716 | MS.gene06269:intron | 30.0% | |
| TCGGTGAAAAAAAAGGAAAT+CGG | + | chr3.1:78386118-78386137 | MS.gene06269:CDS | 30.0% | |
| ! | AATGAAATTAAAAGCATGGC+AGG | + | chr3.1:78386885-78386904 | MS.gene06269:CDS | 30.0% |
| ! | AGAATAGGAGAAAGGATTTT+TGG | - | chr3.1:78385899-78385918 | None:intergenic | 30.0% |
| ! | CATTTGCTGATAACTACAAA+AGG | - | chr3.1:78386693-78386712 | None:intergenic | 30.0% |
| ! | GAATAGGAGAAAGGATTTTT+GGG | - | chr3.1:78385898-78385917 | None:intergenic | 30.0% |
| !! | GGCTATTTTCACTTTGATAT+AGG | + | chr3.1:78385215-78385234 | MS.gene06269:CDS | 30.0% |
| !! | TGAAAAGTGCTTATCATATC+AGG | + | chr3.1:78386487-78386506 | MS.gene06269:intron | 30.0% |
| !!! | TCACATTCCCATTTTTAGTT+GGG | - | chr3.1:78385542-78385561 | None:intergenic | 30.0% |
| !!! | TTCACATTCCCATTTTTAGT+TGG | - | chr3.1:78385543-78385562 | None:intergenic | 30.0% |
| AAAAAGGAAATCGGAAAGCA+AGG | + | chr3.1:78386127-78386146 | MS.gene06269:CDS | 35.0% | |
| AAGAAGAAGCTTCTCAAAGA+AGG | + | chr3.1:78386154-78386173 | MS.gene06269:CDS | 35.0% | |
| AAGAAGACCCCAACTAAAAA+TGG | + | chr3.1:78385531-78385550 | MS.gene06269:CDS | 35.0% | |
| AAGTGTGAGCAACAAAATAG+TGG | - | chr3.1:78385846-78385865 | None:intergenic | 35.0% | |
| AGAAGACCCCAACTAAAAAT+GGG | + | chr3.1:78385532-78385551 | MS.gene06269:CDS | 35.0% | |
| AGTTTGATTCTAGCAGAGAT+AGG | + | chr3.1:78386316-78386335 | MS.gene06269:CDS | 35.0% | |
| ATAATAGATAGGCAGATGGA+AGG | - | chr3.1:78386532-78386551 | None:intergenic | 35.0% | |
| ATAGGTCAAGAATAGGAGAA+AGG | - | chr3.1:78385907-78385926 | None:intergenic | 35.0% | |
| ATGCTTTAACGCATTTCGAA+TGG | + | chr3.1:78385402-78385421 | MS.gene06269:intron | 35.0% | |
| GATAATCCAATTCTCAAGGT+CGG | + | chr3.1:78386099-78386118 | MS.gene06269:CDS | 35.0% | |
| GTTTGATTCTAGCAGAGATA+GGG | + | chr3.1:78386317-78386336 | MS.gene06269:CDS | 35.0% | |
| TTCAATTTCACTCTTGGACA+AGG | + | chr3.1:78386348-78386367 | MS.gene06269:CDS | 35.0% | |
| TTCACTTTGATATAGGCAAG+AGG | + | chr3.1:78385222-78385241 | MS.gene06269:CDS | 35.0% | |
| TTTCTCCTATTCTTGACCTA+TGG | + | chr3.1:78385906-78385925 | MS.gene06269:intron | 35.0% | |
| TTTGATTCTAGCAGAGATAG+GGG | + | chr3.1:78386318-78386337 | MS.gene06269:CDS | 35.0% | |
| ! | CATTTTCACTTCTTCCTCAT+CGG | - | chr3.1:78385622-78385641 | None:intergenic | 35.0% |
| ! | TGTTGAAGTGTTTACCAGTT+AGG | - | chr3.1:78385961-78385980 | None:intergenic | 35.0% |
| !! | GCTGCTTACTTTTGATATTG+TGG | + | chr3.1:78386811-78386830 | MS.gene06269:intron | 35.0% |
| !!! | CACATTCCCATTTTTAGTTG+GGG | - | chr3.1:78385541-78385560 | None:intergenic | 35.0% |
| !!! | TTTTGGGTTGGAAACAAAAC+AGG | - | chr3.1:78385882-78385901 | None:intergenic | 35.0% |
| AAGCTTCTCAAAGAAGGTGA+AGG | + | chr3.1:78386160-78386179 | MS.gene06269:CDS | 40.0% | |
| CAAGAAGGAGGAAAGTAGTT+TGG | + | chr3.1:78385331-78385350 | MS.gene06269:CDS | 40.0% | |
| CGTACTATTATCAGGCAAGT+GGG | + | chr3.1:78386925-78386944 | MS.gene06269:CDS | 40.0% | |
| CTCAAGGTCGGTGAAAAAAA+AGG | + | chr3.1:78386111-78386130 | MS.gene06269:CDS | 40.0% | |
| CTTGAGAATTGGATTATCGC+CGG | - | chr3.1:78386097-78386116 | None:intergenic | 40.0% | |
| GAGATAGGATCCCTATTATG+AGG | + | chr3.1:78386712-78386731 | MS.gene06269:intron | 40.0% | |
| GCCTGATAATAGTACGATGT+GGG | - | chr3.1:78386921-78386940 | None:intergenic | 40.0% | |
| GTGGGTACTAGAGAATTCAT+AGG | + | chr3.1:78386943-78386962 | MS.gene06269:CDS | 40.0% | |
| TATACTGGAACGTTGCTTGA+TGG | + | chr3.1:78386288-78386307 | MS.gene06269:CDS | 40.0% | |
| TCCCACATCGTACTATTATC+AGG | + | chr3.1:78386917-78386936 | MS.gene06269:CDS | 40.0% | |
| TCGTACTATTATCAGGCAAG+TGG | + | chr3.1:78386924-78386943 | MS.gene06269:CDS | 40.0% | |
| TCTCAAAGAAGGTGAAGGTT+GGG | + | chr3.1:78386165-78386184 | MS.gene06269:intron | 40.0% | |
| TCTTTCATGGAATCAACCAC+AGG | - | chr3.1:78386054-78386073 | None:intergenic | 40.0% | |
| TGCCTGATAATAGTACGATG+TGG | - | chr3.1:78386922-78386941 | None:intergenic | 40.0% | |
| TTAGTTGGGGTCTTCTTTGA+AGG | - | chr3.1:78385528-78385547 | None:intergenic | 40.0% | |
| TTCTCAAAGAAGGTGAAGGT+TGG | + | chr3.1:78386164-78386183 | MS.gene06269:intron | 40.0% | |
| TTTACCAGTTAGGCAGTGAT+AGG | - | chr3.1:78385951-78385970 | None:intergenic | 40.0% | |
| ! | TTTTCACCGACCTTGAGAAT+TGG | - | chr3.1:78386108-78386127 | None:intergenic | 40.0% |
| !!! | AGGAGAAAGGATTTTTGGGT+TGG | - | chr3.1:78385894-78385913 | None:intergenic | 40.0% |
| AAAGAAGCAAGGGGTAGAAG+AGG | + | chr3.1:78385277-78385296 | MS.gene06269:CDS | 45.0% | |
| AGGTTAGTGCATTGGAACGA+CGG | + | chr3.1:78386368-78386387 | MS.gene06269:intron | 45.0% | |
| AGTGAGTGCAAAGAAGCAAG+GGG | + | chr3.1:78385268-78385287 | MS.gene06269:CDS | 45.0% | |
| CAGTGAGTGCAAAGAAGCAA+GGG | + | chr3.1:78385267-78385286 | MS.gene06269:CDS | 45.0% | |
| CGGCGATAATCCAATTCTCA+AGG | + | chr3.1:78386095-78386114 | MS.gene06269:CDS | 45.0% | |
| CTTGGACAAGGTTAGTGCAT+TGG | + | chr3.1:78386360-78386379 | MS.gene06269:intron | 45.0% | |
| CTTTGATATAGGCAAGAGGC+AGG | + | chr3.1:78385226-78385245 | MS.gene06269:CDS | 45.0% | |
| GAAGGAGGAAAGTAGTTTGG+AGG | + | chr3.1:78385334-78385353 | MS.gene06269:CDS | 45.0% | |
| GAAGTCGAAGTCGTCTTTCA+TGG | - | chr3.1:78386067-78386086 | None:intergenic | 45.0% | |
| GTTGAAGGTAGTCAAGAAGG+AGG | + | chr3.1:78385319-78385338 | MS.gene06269:CDS | 45.0% | |
| TAGGCAGATGGAAGGTGATA+AGG | - | chr3.1:78386524-78386543 | None:intergenic | 45.0% | |
| TAGTTTGGAGGAATCCTCTG+AGG | + | chr3.1:78385346-78385365 | MS.gene06269:CDS | 45.0% | |
| TCCAGCCATAGGTCAAGAAT+AGG | - | chr3.1:78385914-78385933 | None:intergenic | 45.0% | |
| TCCTATTCTTGACCTATGGC+TGG | + | chr3.1:78385910-78385929 | MS.gene06269:intron | 45.0% | |
| ! | AGAGTCAGATTCCTCTGAAG+TGG | - | chr3.1:78385583-78385602 | None:intergenic | 45.0% |
| ! | TACCTTCAACGCCTTTTGCT+TGG | - | chr3.1:78385309-78385328 | None:intergenic | 45.0% |
| AATTGGATTATCGCCGGCAC+AGG | - | chr3.1:78386091-78386110 | None:intergenic | 50.0% | |
| CACTTCTTCCTCATCGGAAG+AGG | - | chr3.1:78385616-78385635 | None:intergenic | 50.0% | |
| CAGTTAGGCAGTGATAGGAG+AGG | - | chr3.1:78385946-78385965 | None:intergenic | 50.0% | |
| CTCATCCTCAGATTCCTCAG+AGG | - | chr3.1:78385363-78385382 | None:intergenic | 50.0% | |
| CTCTCCTATCACTGCCTAAC+TGG | + | chr3.1:78385944-78385963 | MS.gene06269:intron | 50.0% | |
| GCAGTGAGTGCAAAGAAGCA+AGG | + | chr3.1:78385266-78385285 | MS.gene06269:CDS | 50.0% | |
| GGAGAGTTTCTTCCAGCCAT+AGG | - | chr3.1:78385925-78385944 | None:intergenic | 50.0% | |
| GGTTGGGATGCTCATGATGT+TGG | + | chr3.1:78386181-78386200 | MS.gene06269:intron | 50.0% | |
| ! | CTCTGAGGAATCTGAGGATG+AGG | + | chr3.1:78385361-78385380 | MS.gene06269:CDS | 50.0% |
| ! | CTTCAACGCCTTTTGCTTGG+CGG | - | chr3.1:78385306-78385325 | None:intergenic | 50.0% |
| ! | GGAATCCTCTGAGGAATCTG+AGG | + | chr3.1:78385355-78385374 | MS.gene06269:CDS | 50.0% |
| ! | GGCGTTGAAGGTAGTCAAGA+AGG | + | chr3.1:78385316-78385335 | MS.gene06269:CDS | 50.0% |
| !! | GATTCCTCTGAAGTGGTCTC+TGG | - | chr3.1:78385576-78385595 | None:intergenic | 50.0% |
| AGAGGTTGCCGCCAAGCAAA+AGG | + | chr3.1:78385295-78385314 | MS.gene06269:CDS | 55.0% | |
| GCAGGCTGAGGAAGAAGTCA+AGG | + | chr3.1:78385244-78385263 | MS.gene06269:CDS | 55.0% | |
| TATAGGCAAGAGGCAGGCTG+AGG | + | chr3.1:78385232-78385251 | MS.gene06269:CDS | 55.0% | |
| TGACAGCTCCTCTTCCGATG+AGG | + | chr3.1:78385605-78385624 | MS.gene06269:CDS | 55.0% | |
| ! | GGAGCAGGAGCTTTTGCAAC+AGG | - | chr3.1:78385507-78385526 | None:intergenic | 55.0% |
| ! | GGGGTCTTCTTTGAAGGAGC+AGG | - | chr3.1:78385522-78385541 | None:intergenic | 55.0% |
| !! | CGCCAAGCAAAAGGCGTTGA+AGG | + | chr3.1:78385304-78385323 | MS.gene06269:CDS | 55.0% |
| !! | CTCTGAAGTGGTCTCTGGCT+GGG | - | chr3.1:78385571-78385590 | None:intergenic | 55.0% |
| CCAGCCAGAGACCACTTCAG+AGG | + | chr3.1:78385569-78385588 | MS.gene06269:CDS | 60.0% | |
| GACTTCGACTTCGCCTGTGC+CGG | + | chr3.1:78386075-78386094 | MS.gene06269:CDS | 60.0% | |
| !! | CCTCTGAAGTGGTCTCTGGC+TGG | - | chr3.1:78385572-78385591 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 78385197 | 78386981 | 78385197 | ID=MS.gene06269 |
| chr3.1 | mRNA | 78385197 | 78386981 | 78385197 | ID=MS.gene06269.t1;Parent=MS.gene06269 |
| chr3.1 | exon | 78385197 | 78385382 | 78385197 | ID=MS.gene06269.t1.exon1;Parent=MS.gene06269.t1 |
| chr3.1 | CDS | 78385197 | 78385382 | 78385197 | ID=cds.MS.gene06269.t1;Parent=MS.gene06269.t1 |
| chr3.1 | exon | 78385501 | 78385632 | 78385501 | ID=MS.gene06269.t1.exon2;Parent=MS.gene06269.t1 |
| chr3.1 | CDS | 78385501 | 78385632 | 78385501 | ID=cds.MS.gene06269.t1;Parent=MS.gene06269.t1 |
| chr3.1 | exon | 78386073 | 78386181 | 78386073 | ID=MS.gene06269.t1.exon3;Parent=MS.gene06269.t1 |
| chr3.1 | CDS | 78386073 | 78386181 | 78386073 | ID=cds.MS.gene06269.t1;Parent=MS.gene06269.t1 |
| chr3.1 | exon | 78386283 | 78386369 | 78386283 | ID=MS.gene06269.t1.exon4;Parent=MS.gene06269.t1 |
| chr3.1 | CDS | 78386283 | 78386369 | 78386283 | ID=cds.MS.gene06269.t1;Parent=MS.gene06269.t1 |
| chr3.1 | exon | 78386509 | 78386564 | 78386509 | ID=MS.gene06269.t1.exon5;Parent=MS.gene06269.t1 |
| chr3.1 | CDS | 78386509 | 78386564 | 78386509 | ID=cds.MS.gene06269.t1;Parent=MS.gene06269.t1 |
| chr3.1 | exon | 78386868 | 78386981 | 78386868 | ID=MS.gene06269.t1.exon6;Parent=MS.gene06269.t1 |
| chr3.1 | CDS | 78386868 | 78386981 | 78386868 | ID=cds.MS.gene06269.t1;Parent=MS.gene06269.t1 |
| Gene Sequence |
| Protein sequence |
