AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41121


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41121.t1 MTR_4g064893 78.378 148 2 2 1 118 227 374 7.52e-72 220
MS.gene41121.t1 MTR_2g036390 47.899 119 45 1 18 119 269 387 1.55e-28 107
MS.gene41121.t1 MTR_8g024240 52.381 105 33 2 18 105 247 351 1.47e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41121.t1 AT2G29410 51.695 118 40 2 18 118 236 353 5.78e-36 127
MS.gene41121.t1 AT3G61940 51.376 109 36 2 18 109 196 304 1.68e-28 106
MS.gene41121.t1 AT3G58810 48.800 125 46 3 18 124 254 378 7.38e-28 105
MS.gene41121.t1 AT3G58810 48.800 125 46 3 18 124 293 417 1.37e-27 105
MS.gene41121.t1 AT2G46800 47.059 119 46 2 18 119 260 378 2.94e-27 104
MS.gene41121.t1 AT2G46800 47.059 119 46 2 18 119 260 378 2.94e-27 104
MS.gene41121.t1 AT2G46800 47.059 119 46 2 18 119 260 378 2.94e-27 104
MS.gene41121.t1 AT2G46800 47.059 119 46 2 18 119 260 378 2.94e-27 104
MS.gene41121.t1 AT2G46800 47.059 119 46 2 18 119 260 378 2.94e-27 104

Find 27 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGATATGATTCAATCTTT+TGG 0.253584 4.1:-35990797 MS.gene41121:CDS
AGGTTCACGATTTGCATGTT+TGG 0.334194 4.1:-35990568 MS.gene41121:CDS
AGATCAACAATAAACCATTC+TGG 0.344512 4.1:+35990740 None:intergenic
GGTTCACGATTTGCATGTTT+GGG 0.363513 4.1:-35990567 MS.gene41121:CDS
TTGAAAATGCACATGTTTCA+AGG 0.384457 4.1:-35990277 MS.gene41121:intron
TGATTGCTGGAGGAGTAATA+TGG 0.423479 4.1:-35990769 MS.gene41121:CDS
AATGTCATTCAGCTGGAAAA+TGG 0.445646 4.1:-35990617 MS.gene41121:CDS
CTTCTTACCATTTGAAAGCA+TGG 0.454505 4.1:+35990871 None:intergenic
AACCTTACCATGCTTTCAAA+TGG 0.457120 4.1:-35990878 MS.gene41121:intron
AAATCAATGGAATTTATGCC+AGG 0.476599 4.1:+35990497 None:intergenic
GTACTACTCTGCCAATGCTT+AGG 0.481166 4.1:-35990682 MS.gene41121:CDS
TGAGATTAATGTCATTCAGC+TGG 0.490788 4.1:-35990624 MS.gene41121:CDS
AAATGGTCTTAGATGTATCA+AGG 0.494424 4.1:-35990600 MS.gene41121:CDS
AATCACAGAAAGTAAATCAA+TGG 0.499089 4.1:+35990484 None:intergenic
GTTTCTATCTTGTCATGTAG+TGG 0.519825 4.1:-35990525 MS.gene41121:CDS
CATGTTTGGGCTATAACAGT+GGG 0.523467 4.1:-35990554 MS.gene41121:CDS
TACCATTTGAAAGCATGGTA+AGG 0.531755 4.1:+35990876 None:intergenic
TAGATGTATCAAGGGAGTGC+AGG 0.541398 4.1:-35990591 MS.gene41121:CDS
TGTCATGTAGTGGCTGAACC+TGG 0.544206 4.1:-35990515 MS.gene41121:CDS
GCATGTTTGGGCTATAACAG+TGG 0.554794 4.1:-35990555 MS.gene41121:CDS
GATTGCTGGAGGAGTAATAT+GGG 0.578367 4.1:-35990768 MS.gene41121:CDS
TAATATGGGCTAAACCAGAA+TGG 0.603141 4.1:-35990754 MS.gene41121:intron
TGAATGACATTAATCTCACT+TGG 0.605618 4.1:+35990629 None:intergenic
AATGGTCTTAGATGTATCAA+GGG 0.613237 4.1:-35990599 MS.gene41121:CDS
ACTACCTAAAATAACTGATG+AGG 0.620549 4.1:-35990903 MS.gene41121:CDS
ATGTATCAAGGGAGTGCAGG+AGG 0.626208 4.1:-35990588 MS.gene41121:CDS
TTTGGAGTGATGATTGCTGG+AGG 0.665023 4.1:-35990779 MS.gene41121:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATAAGGTGAAAATAA+TGG + chr4.1:35990605-35990624 None:intergenic 15.0%
!!! TTTTGAATATAAATATCCAA+GGG - chr4.1:35990149-35990168 MS.gene41121:intron 15.0%
!! TTTGAATATAAATATCCAAG+GGG - chr4.1:35990150-35990169 MS.gene41121:intron 20.0%
!!! GTTTTGAATATAAATATCCA+AGG - chr4.1:35990148-35990167 MS.gene41121:intron 20.0%
! AATCACAGAAAGTAAATCAA+TGG + chr4.1:35990506-35990525 None:intergenic 25.0%
! ATACAGCTGTCTTATAAAAT+TGG + chr4.1:35990742-35990761 None:intergenic 25.0%
! CGTCAAACAAACAATTAATA+AGG + chr4.1:35990617-35990636 None:intergenic 25.0%
!! ACCAAATAGTTGCATTTTAA+AGG + chr4.1:35990839-35990858 None:intergenic 25.0%
!! ACTGATATGATTCAATCTTT+TGG - chr4.1:35990190-35990209 MS.gene41121:intron 25.0%
!! CCAAATAGTTGCATTTTAAA+GGG + chr4.1:35990838-35990857 None:intergenic 25.0%
!!! GAATATTTTTGGCATTCTAA+TGG - chr4.1:35990327-35990346 MS.gene41121:intron 25.0%
!!! TCAACTATTTGATTTTGCTA+AGG - chr4.1:35990886-35990905 MS.gene41121:CDS 25.0%
AAATCAATGGAATTTATGCC+AGG + chr4.1:35990493-35990512 None:intergenic 30.0%
ACTACCTAAAATAACTGATG+AGG - chr4.1:35990084-35990103 MS.gene41121:intron 30.0%
AGATCAACAATAAACCATTC+TGG + chr4.1:35990250-35990269 None:intergenic 30.0%
CCAAAAATATTCCTAAGCAT+TGG + chr4.1:35990319-35990338 None:intergenic 30.0%
GCTGTATCAAACAACATATT+CGG - chr4.1:35990755-35990774 MS.gene41121:CDS 30.0%
GTAACGATACAAATTGAGTA+AGG - chr4.1:35990562-35990581 MS.gene41121:CDS 30.0%
TGAATGACATTAATCTCACT+TGG + chr4.1:35990361-35990380 None:intergenic 30.0%
TTGAAAATGCACATGTTTCA+AGG - chr4.1:35990710-35990729 MS.gene41121:intron 30.0%
! AAATGGTCTTAGATGTATCA+AGG - chr4.1:35990387-35990406 MS.gene41121:intron 30.0%
! AATGGTCTTAGATGTATCAA+GGG - chr4.1:35990388-35990407 MS.gene41121:intron 30.0%
! CCAATGCTTAGGAATATTTT+TGG - chr4.1:35990316-35990335 MS.gene41121:intron 30.0%
!! ACTATTTGATTTTGCTAAGG+AGG - chr4.1:35990889-35990908 MS.gene41121:CDS 30.0%
!! CCCTTTAAAATGCAACTATT+TGG - chr4.1:35990835-35990854 MS.gene41121:intron 30.0%
!! TTCTCCTCATCAGTTATTTT+AGG + chr4.1:35990091-35990110 None:intergenic 30.0%
AACCTTACCATGCTTTCAAA+TGG - chr4.1:35990109-35990128 MS.gene41121:intron 35.0%
AATGTCATTCAGCTGGAAAA+TGG - chr4.1:35990370-35990389 MS.gene41121:intron 35.0%
CTTCTTACCATTTGAAAGCA+TGG + chr4.1:35990119-35990138 None:intergenic 35.0%
GTTTCTATCTTGTCATGTAG+TGG - chr4.1:35990462-35990481 MS.gene41121:intron 35.0%
TGAGATTAATGTCATTCAGC+TGG - chr4.1:35990363-35990382 MS.gene41121:intron 35.0%
! TAATATGGGCTAAACCAGAA+TGG - chr4.1:35990233-35990252 MS.gene41121:intron 35.0%
! TACCATTTGAAAGCATGGTA+AGG + chr4.1:35990114-35990133 None:intergenic 35.0%
!! CGGTCATTTTATTCGCTATT+TGG - chr4.1:35990775-35990794 MS.gene41121:CDS 35.0%
!!! ACATGTGCATTTTCAAGTAC+TGG + chr4.1:35990706-35990725 None:intergenic 35.0%
AGGTTCACGATTTGCATGTT+TGG - chr4.1:35990419-35990438 MS.gene41121:intron 40.0%
CATGTTTGGGCTATAACAGT+GGG - chr4.1:35990433-35990452 MS.gene41121:intron 40.0%
CATTGGCAGAGTAGTACTTA+AGG + chr4.1:35990302-35990321 None:intergenic 40.0%
GATTGCTGGAGGAGTAATAT+GGG - chr4.1:35990219-35990238 MS.gene41121:intron 40.0%
GGTTCACGATTTGCATGTTT+GGG - chr4.1:35990420-35990439 MS.gene41121:intron 40.0%
TGATTGCTGGAGGAGTAATA+TGG - chr4.1:35990218-35990237 MS.gene41121:intron 40.0%
!!! TCTTTTGGAGTGATGATTGC+TGG - chr4.1:35990205-35990224 MS.gene41121:intron 40.0%
GCATGTTTGGGCTATAACAG+TGG - chr4.1:35990432-35990451 MS.gene41121:intron 45.0%
GTACTACTCTGCCAATGCTT+AGG - chr4.1:35990305-35990324 MS.gene41121:intron 45.0%
TCACATGCAAATAAGCCCCT+TGG + chr4.1:35990168-35990187 None:intergenic 45.0%
! TAGATGTATCAAGGGAGTGC+AGG - chr4.1:35990396-35990415 MS.gene41121:intron 45.0%
! TTTGGAGTGATGATTGCTGG+AGG - chr4.1:35990208-35990227 MS.gene41121:intron 45.0%
TGTCATGTAGTGGCTGAACC+TGG - chr4.1:35990472-35990491 MS.gene41121:intron 50.0%
! ATGTATCAAGGGAGTGCAGG+AGG - chr4.1:35990399-35990418 MS.gene41121:intron 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 35990076 35990933 35990076 ID=MS.gene41121
chr4.1 mRNA 35990076 35990933 35990076 ID=MS.gene41121.t1;Parent=MS.gene41121
chr4.1 exon 35990879 35990933 35990879 ID=MS.gene41121.t1.exon1;Parent=MS.gene41121.t1
chr4.1 CDS 35990879 35990933 35990879 ID=cds.MS.gene41121.t1;Parent=MS.gene41121.t1
chr4.1 exon 35990755 35990839 35990755 ID=MS.gene41121.t1.exon2;Parent=MS.gene41121.t1
chr4.1 CDS 35990755 35990839 35990755 ID=cds.MS.gene41121.t1;Parent=MS.gene41121.t1
chr4.1 exon 35990490 35990703 35990490 ID=MS.gene41121.t1.exon3;Parent=MS.gene41121.t1
chr4.1 CDS 35990490 35990703 35990490 ID=cds.MS.gene41121.t1;Parent=MS.gene41121.t1
chr4.1 exon 35990278 35990311 35990278 ID=MS.gene41121.t1.exon4;Parent=MS.gene41121.t1
chr4.1 CDS 35990278 35990311 35990278 ID=cds.MS.gene41121.t1;Parent=MS.gene41121.t1
chr4.1 exon 35990076 35990101 35990076 ID=MS.gene41121.t1.exon5;Parent=MS.gene41121.t1
chr4.1 CDS 35990076 35990101 35990076 ID=cds.MS.gene41121.t1;Parent=MS.gene41121.t1
Gene Sequence

>MS.gene41121

ATGGAGGAACTACCTAAAATAACTGATGAGGAGAACCTTACCATGCTTTCAAATGGTAAGAAGAATACTAATGTTTTGAATATAAATATCCAAGGGGCTTATTTGCATGTGATGACTGATATGATTCAATCTTTTGGAGTGATGATTGCTGGAGGAGTAATATGGGCTAAACCAGAATGGTTTATTGTTGATCTTTTATGTACTCTAATATTCTCTGTTTTATCCTTAAGTACTACTCTGCCAATGCTTAGGAATATTTTTGGCATTCTAATGGAGAGAACGCCAAGTGAGATTAATGTCATTCAGCTGGAAAATGGTCTTAGATGTATCAAGGGAGTGCAGGAGGTTCACGATTTGCATGTTTGGGCTATAACAGTGGGAAAACTGTTTCTATCTTGTCATGTAGTGGCTGAACCTGGCATAAATTCCATTGATTTACTTTCTGTGATTAAACAATATTGTGAAAACACTTATCAAATACAACATGTAACGATACAAATTGAGTAAGGTTATATTCTTTTCTGTTCCATTATTTTCACCTTATTAATTGTTTGTTTGACGCATACTCATATAGATTCACAAGACGTGCTTTATTCTATTCTATTTTGACTTTGAACAACAGAAATACCAGTACTTGAAAATGCACATGTTTCAAGGTCATAACCAATTTTATAAGACAGCTGTATCAAACAACATATTCGGTCATTTTATTCGCTATTTGGTCATTTTGATGTTCAATCAAACAACATATTTCTTGTTCCCTTTAAAATGCAACTATTTGGTTTTGCTATTTCTCGTTCTATTTAAAATTCAACTATTTGATTTTGCTAAGGAGGAGCGAAACACCTTGAAAAGTAG

Protein sequence

>MS.gene41121.t1

MEELPKITDEENLTMLSNGAYLHVMTDMIQSFGVMIAGGVIWAKPECTTLPMLRNIFGILMERTPSEINVIQLENGLRCIKGVQEVHDLHVWAITVGKLFLSCHVVAEPGINSIDLLSKYQYLKMHMFQGGAKHLEK