AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060374


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060374.t1 MTR_4g118320 99.133 346 3 0 1 346 1 346 0.0 719
MS.gene060374.t1 MTR_2g020990 75.568 352 72 5 1 344 1 346 0.0 536
MS.gene060374.t1 MTR_0003s0660 40.936 342 178 7 5 340 55 378 1.10e-78 246
MS.gene060374.t1 MTR_0076s0020 43.391 348 170 9 1 339 1 330 7.55e-78 243
MS.gene060374.t1 MTR_7g018290 40.762 341 178 7 5 339 9 331 9.79e-78 243
MS.gene060374.t1 MTR_8g069905 41.379 348 177 10 1 339 3 332 1.93e-71 227
MS.gene060374.t1 MTR_7g018310 39.474 342 162 7 5 340 55 357 4.04e-70 224
MS.gene060374.t1 MTR_7g018320 38.643 339 138 7 5 339 55 327 2.61e-62 202
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060374.t1 AT5G18930 62.817 355 113 8 1 344 1 347 2.96e-153 434
MS.gene060374.t1 AT3G02470 41.667 348 178 9 1 339 1 332 1.45e-82 255
MS.gene060374.t1 AT3G02470 41.667 348 178 9 1 339 1 332 1.45e-82 255
MS.gene060374.t1 AT3G02470 41.667 348 178 9 1 339 1 332 1.45e-82 255
MS.gene060374.t1 AT3G25570 43.931 346 174 8 1 339 1 333 1.03e-79 247
MS.gene060374.t1 AT3G25570 43.931 346 174 8 1 339 1 333 1.03e-79 247
MS.gene060374.t1 AT5G15950 43.046 302 151 5 1 296 3 289 8.10e-77 240
MS.gene060374.t1 AT5G15950 43.046 302 151 5 1 296 3 289 8.10e-77 240
MS.gene060374.t1 AT3G17717 35.593 118 66 2 182 296 17 127 2.75e-17 77.8

Find 67 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATAAAGTGAGGCCTATT+TGG 0.238267 8.4:+13031742 None:intergenic
CGGTTGGAACTTCATTTCTT+TGG 0.257423 8.4:-13031996 MS.gene060374:CDS
TGGTTTGCATAGTGAATTAA+AGG 0.281734 8.4:+13031762 None:intergenic
TCGGTTTCCCGCGGCGGATA+TGG 0.292619 8.4:-13031052 MS.gene060374:CDS
AGGGATAGTGTCTTCTAAAT+AGG 0.294020 8.4:+13031641 None:intergenic
ATTCACTATGCAAACCAAAT+AGG 0.302334 8.4:-13031756 MS.gene060374:CDS
AAAGAGATGACGGAGCTTAC+TGG 0.320761 8.4:-13031393 MS.gene060374:CDS
ACACCCCAAATGGCTGTATC+TGG 0.329316 8.4:-13032038 None:intergenic
AAGGGAAAGGTTGTGCATTT+GGG 0.344982 8.4:+13031688 None:intergenic
TTCTAGCTCGGAAATTCTTC+CGG 0.362591 8.4:-13031452 MS.gene060374:CDS
AAGTCACAAATGAATGCATT+TGG 0.369954 8.4:+13031354 None:intergenic
TAAGGGAAAGGTTGTGCATT+TGG 0.385623 8.4:+13031687 None:intergenic
AAGAATGGATTTGAGAAGTT+GGG 0.391105 8.4:+13031785 None:intergenic
GAAGAATGGATTTGAGAAGT+TGG 0.397282 8.4:+13031784 None:intergenic
AAGAATTTCCGAGCTAGAAT+AGG 0.404065 8.4:+13031456 None:intergenic
TGATCCAATCACCTTCCAAC+TGG 0.405376 8.4:-13031970 MS.gene060374:CDS
AGACCCAGTTGGAAGGTGAT+TGG 0.409794 8.4:+13031966 None:intergenic
ATGGCTGTATCTGGCTTTGA+AGG 0.409980 8.4:-13032029 MS.gene060374:CDS
GCATAGCTGAAACCATCTTC+TGG 0.410267 8.4:+13031261 None:intergenic
TTTCTATGGCAAAGATTTGA+AGG 0.430183 8.4:+13031621 None:intergenic
TCGTCATTGAAGCTTGTGTA+AGG 0.431177 8.4:+13031669 None:intergenic
TGCACAGTGGTTTCAGCAGT+TGG 0.431217 8.4:-13031894 MS.gene060374:CDS
ATAACATAGTACATGTGTTA+AGG 0.454483 8.4:-13031209 MS.gene060374:CDS
AGAATTTCCGAGCTAGAATA+GGG 0.459819 8.4:+13031457 None:intergenic
TGAGTGTGAAGAGGATTTAG+AGG 0.469691 8.4:+13031584 None:intergenic
CGGAAATTCTTCCGGCCACC+CGG 0.469974 8.4:-13031444 MS.gene060374:CDS
GTGAATGCAGTCGAGCCTCT+TGG 0.477557 8.4:-13031102 MS.gene060374:CDS
ATAGTGAATTAAAGGAAGAA+TGG 0.485437 8.4:+13031770 None:intergenic
AGAGGATTTAGAGGGCATGA+TGG 0.487479 8.4:+13031593 None:intergenic
CCTTGTGGATATTCAATGAA+TGG 0.491016 8.4:-13031321 MS.gene060374:CDS
TTCTTCCGGCCACCCGGAGA+TGG 0.498491 8.4:-13031438 MS.gene060374:CDS
GCTTTGAAGGATTTGAGAAA+CGG 0.501692 8.4:-13032016 MS.gene060374:CDS
ATTCATGTTACACCAGAAGA+TGG 0.501825 8.4:-13031273 MS.gene060374:CDS
GACTTTGCATTTGATCCTTG+TGG 0.508004 8.4:-13031336 MS.gene060374:CDS
GCCGGCATTAAACATTTCTC+CGG 0.515988 8.4:+13031004 None:intergenic
TGAAGGATTTGAGAAACGGT+TGG 0.520330 8.4:-13032012 MS.gene060374:CDS
GTAGGAGTCGTGCTGTCGAT+CGG 0.527608 8.4:-13031071 MS.gene060374:CDS
CTAAATCCTCTTCACACTCA+TGG 0.535095 8.4:-13031581 MS.gene060374:CDS
TGCTCTTGTCGCTACACAAG+AGG 0.538275 8.4:-13031723 MS.gene060374:CDS
TGAATGGAATGGATGATGAT+TGG 0.540234 8.4:-13031305 MS.gene060374:CDS
TTGAAGCTTGTGTAAGGGAA+AGG 0.548951 8.4:+13031675 None:intergenic
CTCTGTCATGCATATCTCCA+TGG 0.549414 8.4:+13031485 None:intergenic
TGGATATTCAATGAATGGAA+TGG 0.555746 8.4:-13031316 MS.gene060374:CDS
TATGCAAGCTTTGAATGCGT+TGG 0.568343 8.4:-13031243 MS.gene060374:CDS
TTCTATGGCAAAGATTTGAA+GGG 0.570945 8.4:+13031622 None:intergenic
TAATGAGATGTTGAAGAAGA+TGG 0.572441 8.4:-13031124 MS.gene060374:CDS
AGCCTCTTGGACTGAAATGT+AGG 0.582524 8.4:-13031089 MS.gene060374:CDS
AGTTAGACCCTATTCTAGCT+CGG 0.587915 8.4:-13031464 MS.gene060374:CDS
AAGATCATAATCAAAACTTG+TGG 0.591582 8.4:-13031813 MS.gene060374:CDS
CGTCATTGAAGCTTGTGTAA+GGG 0.591601 8.4:+13031670 None:intergenic
GATCCAATCACCTTCCAACT+GGG 0.591867 8.4:-13031969 MS.gene060374:CDS
CTCAGCCGGAAAAGAGATGA+CGG 0.594514 8.4:-13031403 MS.gene060374:CDS
ATCTTCTGGTGTAACATGAA+TGG 0.598130 8.4:+13031275 None:intergenic
CTCCTACATTTCAGTCCAAG+AGG 0.608780 8.4:+13031087 None:intergenic
TCTTAGTTGGATAAACAAAG+AGG 0.610861 8.4:+13031832 None:intergenic
CCATTCATTGAATATCCACA+AGG 0.611208 8.4:+13031321 None:intergenic
AAAACAACCATATCCGCCGC+GGG 0.620904 8.4:+13031045 None:intergenic
TGCTGTCGATCGGTTTCCCG+CGG 0.625722 8.4:-13031061 MS.gene060374:CDS
TGTCGATCGGTTTCCCGCGG+CGG 0.636214 8.4:-13031058 MS.gene060374:CDS
TTCTGGTGTAACATGAATGG+TGG 0.639028 8.4:+13031278 None:intergenic
GAGTGTGAAGAGGATTTAGA+GGG 0.647633 8.4:+13031585 None:intergenic
ATATAGACATAGTACCTGGT+CGG 0.655930 8.4:+13031169 None:intergenic
AACATGCCATGAGTGTGAAG+AGG 0.667723 8.4:+13031575 None:intergenic
ATTGATATAGACATAGTACC+TGG 0.670482 8.4:+13031165 None:intergenic
TGACAGTGATATCTTCACCA+TGG 0.676549 8.4:-13031502 MS.gene060374:CDS
AGAAGCTGTTCAATGCACAG+TGG 0.728078 8.4:-13031907 MS.gene060374:CDS
AGCGACAAGAGCATAAAGTG+AGG 0.728752 8.4:+13031733 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGATCATAATCAAAACTTG+TGG - chr8.4:13031227-13031246 MS.gene060374:CDS 25.0%
! ATAACATAGTACATGTGTTA+AGG - chr8.4:13031831-13031850 MS.gene060374:CDS 25.0%
! ATAGTGAATTAAAGGAAGAA+TGG + chr8.4:13031273-13031292 None:intergenic 25.0%
!!! GGTTGTTTTTCAAACTTTTA+CGG - chr8.4:13032009-13032028 MS.gene060374:CDS 25.0%
!!! GTTTTGATTATGATCTTAGT+TGG + chr8.4:13031224-13031243 None:intergenic 25.0%
AAGTCACAAATGAATGCATT+TGG + chr8.4:13031689-13031708 None:intergenic 30.0%
ATTCACTATGCAAACCAAAT+AGG - chr8.4:13031284-13031303 MS.gene060374:CDS 30.0%
ATTGATATAGACATAGTACC+TGG + chr8.4:13031878-13031897 None:intergenic 30.0%
TAATGAGATGTTGAAGAAGA+TGG - chr8.4:13031916-13031935 MS.gene060374:CDS 30.0%
TCTTAGTTGGATAAACAAAG+AGG + chr8.4:13031211-13031230 None:intergenic 30.0%
TGGATATTCAATGAATGGAA+TGG - chr8.4:13031724-13031743 MS.gene060374:CDS 30.0%
TTCTATGGCAAAGATTTGAA+GGG + chr8.4:13031421-13031440 None:intergenic 30.0%
TTTCTATGGCAAAGATTTGA+AGG + chr8.4:13031422-13031441 None:intergenic 30.0%
! AAGAATGGATTTGAGAAGTT+GGG + chr8.4:13031258-13031277 None:intergenic 30.0%
! TGGTTTGCATAGTGAATTAA+AGG + chr8.4:13031281-13031300 None:intergenic 30.0%
AAGAATTTCCGAGCTAGAAT+AGG + chr8.4:13031587-13031606 None:intergenic 35.0%
AGAATTTCCGAGCTAGAATA+GGG + chr8.4:13031586-13031605 None:intergenic 35.0%
AGGGATAGTGTCTTCTAAAT+AGG + chr8.4:13031402-13031421 None:intergenic 35.0%
ATATAGACATAGTACCTGGT+CGG + chr8.4:13031874-13031893 None:intergenic 35.0%
ATCTTCTGGTGTAACATGAA+TGG + chr8.4:13031768-13031787 None:intergenic 35.0%
ATTCATGTTACACCAGAAGA+TGG - chr8.4:13031767-13031786 MS.gene060374:CDS 35.0%
CCATTCATTGAATATCCACA+AGG + chr8.4:13031722-13031741 None:intergenic 35.0%
CCTTGTGGATATTCAATGAA+TGG - chr8.4:13031719-13031738 MS.gene060374:CDS 35.0%
TGAATGGAATGGATGATGAT+TGG - chr8.4:13031735-13031754 MS.gene060374:CDS 35.0%
! CAATTTTTCTTAGACCCAGT+TGG + chr8.4:13031088-13031107 None:intergenic 35.0%
! GAAGAATGGATTTGAGAAGT+TGG + chr8.4:13031259-13031278 None:intergenic 35.0%
!! GCTTTGAAGGATTTGAGAAA+CGG - chr8.4:13031024-13031043 MS.gene060374:CDS 35.0%
AAGGGAAAGGTTGTGCATTT+GGG + chr8.4:13031355-13031374 None:intergenic 40.0%
AGCATAAAGTGAGGCCTATT+TGG + chr8.4:13031301-13031320 None:intergenic 40.0%
CGGTTGGAACTTCATTTCTT+TGG - chr8.4:13031044-13031063 MS.gene060374:CDS 40.0%
CGTCATTGAAGCTTGTGTAA+GGG + chr8.4:13031373-13031392 None:intergenic 40.0%
CTAAATCCTCTTCACACTCA+TGG - chr8.4:13031459-13031478 MS.gene060374:CDS 40.0%
GACTTTGCATTTGATCCTTG+TGG - chr8.4:13031704-13031723 MS.gene060374:CDS 40.0%
GAGTGTGAAGAGGATTTAGA+GGG + chr8.4:13031458-13031477 None:intergenic 40.0%
TAAGGGAAAGGTTGTGCATT+TGG + chr8.4:13031356-13031375 None:intergenic 40.0%
TCGTCATTGAAGCTTGTGTA+AGG + chr8.4:13031374-13031393 None:intergenic 40.0%
TGAAGGATTTGAGAAACGGT+TGG - chr8.4:13031028-13031047 MS.gene060374:CDS 40.0%
TGACAGTGATATCTTCACCA+TGG - chr8.4:13031538-13031557 MS.gene060374:CDS 40.0%
TGAGTGTGAAGAGGATTTAG+AGG + chr8.4:13031459-13031478 None:intergenic 40.0%
TTCTGGTGTAACATGAATGG+TGG + chr8.4:13031765-13031784 None:intergenic 40.0%
TTGAAGCTTGTGTAAGGGAA+AGG + chr8.4:13031368-13031387 None:intergenic 40.0%
! AGTTAGACCCTATTCTAGCT+CGG - chr8.4:13031576-13031595 MS.gene060374:CDS 40.0%
! TTTTCTTAGACCCAGTTGGA+AGG + chr8.4:13031084-13031103 None:intergenic 40.0%
!! GCATGATGGAAGCTTTTCTA+TGG + chr8.4:13031436-13031455 None:intergenic 40.0%
!! TATGCAAGCTTTGAATGCGT+TGG - chr8.4:13031797-13031816 MS.gene060374:CDS 40.0%
!! TTCTAGCTCGGAAATTCTTC+CGG - chr8.4:13031588-13031607 MS.gene060374:CDS 40.0%
AAAAACAACCATATCCGCCG+CGG + chr8.4:13031999-13032018 None:intergenic 45.0%
AACATGCCATGAGTGTGAAG+AGG + chr8.4:13031468-13031487 None:intergenic 45.0%
AGAAGCTGTTCAATGCACAG+TGG - chr8.4:13031133-13031152 MS.gene060374:CDS 45.0%
AGCCTCTTGGACTGAAATGT+AGG - chr8.4:13031951-13031970 MS.gene060374:CDS 45.0%
CTCCTACATTTCAGTCCAAG+AGG + chr8.4:13031956-13031975 None:intergenic 45.0%
CTCTGTCATGCATATCTCCA+TGG + chr8.4:13031558-13031577 None:intergenic 45.0%
GATCCAATCACCTTCCAACT+GGG - chr8.4:13031071-13031090 MS.gene060374:CDS 45.0%
GCATAGCTGAAACCATCTTC+TGG + chr8.4:13031782-13031801 None:intergenic 45.0%
TGATCCAATCACCTTCCAAC+TGG - chr8.4:13031070-13031089 MS.gene060374:CDS 45.0%
! AAGCTCCGTCATCTCTTTTC+CGG + chr8.4:13031645-13031664 None:intergenic 45.0%
! AGAGGATTTAGAGGGCATGA+TGG + chr8.4:13031450-13031469 None:intergenic 45.0%
! AGCGACAAGAGCATAAAGTG+AGG + chr8.4:13031310-13031329 None:intergenic 45.0%
! GTTGTTCAGATTTTCCGACC+AGG - chr8.4:13031857-13031876 MS.gene060374:CDS 45.0%
! TTCAAACTTTTACGGCTCGC+CGG - chr8.4:13032017-13032036 MS.gene060374:CDS 45.0%
!! AAAGAGATGACGGAGCTTAC+TGG - chr8.4:13031647-13031666 MS.gene060374:CDS 45.0%
!! ATGGCTGTATCTGGCTTTGA+AGG - chr8.4:13031011-13031030 MS.gene060374:CDS 45.0%
AAAACAACCATATCCGCCGC+GGG + chr8.4:13031998-13032017 None:intergenic 50.0%
AGACCCAGTTGGAAGGTGAT+TGG + chr8.4:13031077-13031096 None:intergenic 50.0%
CTCAGCCGGAAAAGAGATGA+CGG - chr8.4:13031637-13031656 MS.gene060374:CDS 50.0%
TGCACAGTGGTTTCAGCAGT+TGG - chr8.4:13031146-13031165 MS.gene060374:CDS 50.0%
TGCTCTTGTCGCTACACAAG+AGG - chr8.4:13031317-13031336 MS.gene060374:CDS 50.0%
!!! GTCACCGGTTTTTCCATCTC+CGG + chr8.4:13031618-13031637 None:intergenic 50.0%
!!! TCACCGGTTTTTCCATCTCC+GGG + chr8.4:13031617-13031636 None:intergenic 50.0%
CCACCCGGAGATGGAAAAAC+CGG - chr8.4:13031611-13031630 MS.gene060374:CDS 55.0%
GGAAAAACCGGTGACTCAGC+CGG - chr8.4:13031623-13031642 MS.gene060374:CDS 55.0%
GTAGGAGTCGTGCTGTCGAT+CGG - chr8.4:13031969-13031988 MS.gene060374:CDS 55.0%
GTGAATGCAGTCGAGCCTCT+TGG - chr8.4:13031938-13031957 MS.gene060374:CDS 55.0%
! CTCTTTTCCGGCTGAGTCAC+CGG + chr8.4:13031633-13031652 None:intergenic 55.0%
! TTTTTCCATCTCCGGGTGGC+CGG + chr8.4:13031610-13031629 None:intergenic 55.0%
CGGAAATTCTTCCGGCCACC+CGG - chr8.4:13031596-13031615 MS.gene060374:CDS 60.0%
! CCGGTTTTTCCATCTCCGGG+TGG + chr8.4:13031614-13031633 None:intergenic 60.0%
! TGCTGTCGATCGGTTTCCCG+CGG - chr8.4:13031979-13031998 MS.gene060374:CDS 60.0%
TCGGTTTCCCGCGGCGGATA+TGG - chr8.4:13031988-13032007 MS.gene060374:CDS 65.0%
TTCTTCCGGCCACCCGGAGA+TGG - chr8.4:13031602-13031621 MS.gene060374:CDS 65.0%
! TGTCGATCGGTTTCCCGCGG+CGG - chr8.4:13031982-13032001 MS.gene060374:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 13031011 13032051 13031011 ID=MS.gene060374
chr8.4 mRNA 13031011 13032051 13031011 ID=MS.gene060374.t1;Parent=MS.gene060374
chr8.4 exon 13031011 13032051 13031011 ID=MS.gene060374.t1.exon1;Parent=MS.gene060374.t1
chr8.4 CDS 13031011 13032051 13031011 ID=cds.MS.gene060374.t1;Parent=MS.gene060374.t1
Gene Sequence

>MS.gene060374

ATGGCTGTATCTGGCTTTGAAGGATTTGAGAAACGGTTGGAACTTCATTTCTTTGGAGATGATCCAATCACCTTCCAACTGGGTCTAAGAAAAATTGATTTTGAATCAATACAACAAGTGTTAGAAGCTGTTCAATGCACAGTGGTTTCAGCAGTTGGAAACTCTTACTTTGATGCATATGTTCTATCAGAATCAAGCCTCTTTGTTTATCCAACTAAGATCATAATCAAAACTTGTGGAACAACCCAACTTCTCAAATCCATTCTTCCTTTAATTCACTATGCAAACCAAATAGGCCTCACTTTATGCTCTTGTCGCTACACAAGAGGAAGCTTCATCTTCCCAAATGCACAACCTTTCCCTTACACAAGCTTCAATGACGAAGTAACCTATTTAGAAGACACTATCCCTTCAAATCTTTGCCATAGAAAAGCTTCCATCATGCCCTCTAAATCCTCTTCACACTCATGGCATGTTTTCACTGCACACTCTTCAATAACTCATCACAATTACAGTCACACTCATAGTGACAGTGATATCTTCACCATGGAGATATGCATGACAGAGTTAGACCCTATTCTAGCTCGGAAATTCTTCCGGCCACCCGGAGATGGAAAAACCGGTGACTCAGCCGGAAAAGAGATGACGGAGCTTACTGGAATCAATGAAATAAATCCAAATGCATTCATTTGTGACTTTGCATTTGATCCTTGTGGATATTCAATGAATGGAATGGATGATGATTGGTATTCCACCATTCATGTTACACCAGAAGATGGTTTCAGCTATGCAAGCTTTGAATGCGTTGGTTCTGTTAACGATAACATAGTACATGTGTTAAGGAAAGTTGTTCAGATTTTCCGACCAGGTACTATGTCTATATCAATGACATGCAGTAATTATAGTAATGAGATGTTGAAGAAGATGGTGAATGCAGTCGAGCCTCTTGGACTGAAATGTAGGAGTCGTGCTGTCGATCGGTTTCCCGCGGCGGATATGGTTGTTTTTCAAACTTTTACGGCTCGCCGGAGAAATGTTTAA

Protein sequence

>MS.gene060374.t1

MAVSGFEGFEKRLELHFFGDDPITFQLGLRKIDFESIQQVLEAVQCTVVSAVGNSYFDAYVLSESSLFVYPTKIIIKTCGTTQLLKSILPLIHYANQIGLTLCSCRYTRGSFIFPNAQPFPYTSFNDEVTYLEDTIPSNLCHRKASIMPSKSSSHSWHVFTAHSSITHHNYSHTHSDSDIFTMEICMTELDPILARKFFRPPGDGKTGDSAGKEMTELTGINEINPNAFICDFAFDPCGYSMNGMDDDWYSTIHVTPEDGFSYASFECVGSVNDNIVHVLRKVVQIFRPGTMSISMTCSNYSNEMLKKMVNAVEPLGLKCRSRAVDRFPAADMVVFQTFTARRRNV