AlfalfaGEDB | MS.gene27812

M. sativa cultivar XinJiangDaYe / MS.gene27812

KEGG: -
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00860.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27812.t1	XP_003629785.1	99.6	492	2	0	3	494	33	524	8.30E-278	965.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27812.t1	Q94C70	83.9	491	79	0	4	494	34	524	1.0e-244	847.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27812.t1	G7LJF6	99.6	492	2	0	3	494	33	524	6.0e-278	965.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene27812	0.814778	1.30E-51	-1.69E-46
MS.gene049989	MS.gene27812	0.816005	6.91E-52	-1.69E-46
MS.gene05054	MS.gene27812	0.802169	6.37E-49	-1.69E-46
MS.gene050707	MS.gene27812	0.811228	7.78E-51	-1.69E-46
MS.gene050875	MS.gene27812	0.811655	6.28E-51	-1.69E-46
MS.gene05124	MS.gene27812	0.807629	4.61E-50	-1.69E-46
MS.gene051816	MS.gene27812	0.847302	1.26E-59	-1.69E-46
MS.gene051896	MS.gene27812	0.813854	2.07E-51	-1.69E-46
MS.gene051914	MS.gene27812	0.840978	6.28E-58	-1.69E-46
MS.gene051955	MS.gene27812	0.866783	2.21E-65	-1.69E-46
MS.gene052143	MS.gene27812	0.829291	5.59E-55	-1.69E-46
MS.gene052308	MS.gene27812	0.886406	3.52E-72	-1.69E-46
MS.gene052367	MS.gene27812	0.861634	8.94E-64	-1.69E-46
MS.gene053173	MS.gene27812	0.866819	2.16E-65	-1.69E-46
MS.gene053348	MS.gene27812	0.870584	1.31E-66	-1.69E-46
MS.gene053416	MS.gene27812	0.809242	2.08E-50	-1.69E-46
MS.gene053417	MS.gene27812	0.806375	8.48E-50	-1.69E-46
MS.gene054551	MS.gene27812	0.882257	1.21E-70	-1.69E-46
MS.gene054552	MS.gene27812	0.828022	1.13E-54	-1.69E-46
MS.gene054593	MS.gene27812	0.878927	1.89E-69	-1.69E-46
MS.gene054614	MS.gene27812	0.816352	5.78E-52	-1.69E-46
MS.gene054633	MS.gene27812	0.855855	4.77E-62	-1.69E-46
MS.gene054662	MS.gene27812	0.835618	1.51E-56	-1.69E-46
MS.gene054850	MS.gene27812	0.823563	1.29E-53	-1.69E-46
MS.gene054885	MS.gene27812	0.832207	1.08E-55	-1.69E-46
MS.gene054941	MS.gene27812	0.831701	1.44E-55	-1.69E-46
MS.gene055308	MS.gene27812	0.907165	6.80E-81	-1.69E-46
MS.gene055382	MS.gene27812	0.819557	1.09E-52	-1.69E-46
MS.gene055578	MS.gene27812	0.894023	3.64E-75	-1.69E-46
MS.gene055579	MS.gene27812	0.837246	5.82E-57	-1.69E-46
MS.gene055957	MS.gene27812	0.854318	1.33E-61	-1.69E-46
MS.gene056041	MS.gene27812	0.888396	6.13E-73	-1.69E-46
MS.gene056042	MS.gene27812	0.820658	6.11E-53	-1.69E-46
MS.gene05640	MS.gene27812	0.888193	7.33E-73	-1.69E-46
MS.gene056526	MS.gene27812	0.812916	3.33E-51	-1.69E-46
MS.gene056528	MS.gene27812	0.829077	6.30E-55	-1.69E-46
MS.gene05652	MS.gene27812	0.864353	1.29E-64	-1.69E-46
MS.gene057367	MS.gene27812	0.84275	2.14E-58	-1.69E-46
MS.gene057458	MS.gene27812	0.865954	4.06E-65	-1.69E-46
MS.gene057669	MS.gene27812	0.834997	2.17E-56	-1.69E-46
MS.gene058013	MS.gene27812	0.824909	6.25E-54	-1.69E-46
MS.gene058128	MS.gene27812	0.902947	5.75E-79	-1.69E-46
MS.gene058827	MS.gene27812	0.838373	2.99E-57	-1.69E-46
MS.gene058828	MS.gene27812	0.819924	9.00E-53	-1.69E-46
MS.gene058853	MS.gene27812	0.970966	1.99E-132	-1.69E-46
MS.gene05926	MS.gene27812	0.800344	1.51E-48	-1.69E-46
MS.gene059553	MS.gene27812	0.857249	1.86E-62	-1.69E-46
MS.gene059616	MS.gene27812	0.857188	1.94E-62	-1.69E-46
MS.gene059905	MS.gene27812	0.802652	5.07E-49	-1.69E-46
MS.gene060374	MS.gene27812	0.809298	2.03E-50	-1.69E-46
MS.gene060473	MS.gene27812	0.866027	3.85E-65	-1.69E-46
MS.gene060565	MS.gene27812	0.854179	1.46E-61	-1.69E-46
MS.gene060825	MS.gene27812	0.819978	8.74E-53	-1.69E-46
MS.gene06093	MS.gene27812	0.882648	8.74E-71	-1.69E-46
MS.gene060985	MS.gene27812	0.807103	5.95E-50	-1.69E-46
MS.gene061099	MS.gene27812	0.858411	8.39E-63	-1.69E-46
MS.gene061260	MS.gene27812	0.882189	1.28E-70	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene27812	MS.gene91317	PPI
MS.gene70718	MS.gene27812	PPI
MS.gene70604	MS.gene27812	PPI
MS.gene27812	MS.gene43290	PPI
MS.gene022275	MS.gene27812	PPI
MS.gene56947	MS.gene27812	PPI
MS.gene27812	MS.gene96701	PPI
MS.gene86930	MS.gene27812	PPI
MS.gene074377	MS.gene27812	PPI
MS.gene044204	MS.gene27812	PPI
MS.gene25932	MS.gene27812	PPI
MS.gene25800	MS.gene27812	PPI
MS.gene71606	MS.gene27812	PPI
MS.gene033221	MS.gene27812	PPI
MS.gene60344	MS.gene27812	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27812.t1	MTR_8g086520	99.391	493	3	0	2	494	32	524	0.0	990
MS.gene27812.t1	MTR_2g016460	83.976	493	79	0	2	494	32	524	0.0	862
MS.gene27812.t1	MTR_8g086520	99.291	423	3	0	2	424	32	454	0.0	841
MS.gene27812.t1	MTR_8g086520	99.502	402	2	0	93	494	7	408	0.0	811
MS.gene27812.t1	MTR_3g080390	72.470	494	135	1	2	494	27	520	0.0	748
MS.gene27812.t1	MTR_8g086520	98.922	371	4	0	2	372	32	402	0.0	733
MS.gene27812.t1	MTR_8g063220	60.649	493	192	1	2	492	39	531	0.0	620
MS.gene27812.t1	MTR_3g078270	60.041	493	195	1	2	492	38	530	0.0	604
MS.gene27812.t1	MTR_4g106750	59.635	493	197	1	2	492	38	530	0.0	599
MS.gene27812.t1	MTR_4g106750	59.583	480	192	1	15	492	2	481	0.0	583
MS.gene27812.t1	MTR_1g021120	57.230	491	210	0	2	492	55	545	0.0	577
MS.gene27812.t1	MTR_5g035180	56.855	496	207	3	2	492	43	536	0.0	573
MS.gene27812.t1	MTR_1g106085	56.098	492	210	3	4	492	37	525	0.0	550
MS.gene27812.t1	MTR_3g103230	52.929	495	224	4	2	492	63	552	0.0	546
MS.gene27812.t1	MTR_5g022110	53.226	496	224	4	1	492	54	545	0.0	525
MS.gene27812.t1	MTR_4g106750	59.783	368	146	1	127	492	11	378	1.96e-158	455
MS.gene27812.t1	MTR_2g084895	36.994	519	289	5	7	491	164	678	3.84e-109	339
MS.gene27812.t1	MTR_8g068550	36.434	516	292	5	8	491	244	755	5.69e-109	341
MS.gene27812.t1	MTR_5g032020	37.403	516	287	5	4	487	182	693	8.47e-107	333
MS.gene27812.t1	MTR_2g103510	33.794	506	289	6	7	491	111	591	1.78e-98	309
MS.gene27812.t1	MTR_4g055290	35.260	519	293	6	7	491	171	680	2.17e-98	311
MS.gene27812.t1	MTR_8g086535	91.089	101	9	0	272	372	18	118	6.02e-54	177
MS.gene27812.t1	MTR_5g042660	64.286	56	20	0	425	480	39	94	1.29e-18	81.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27812.t1	AT2G34190	83.570	493	81	0	2	494	32	524	0.0	865
MS.gene27812.t1	AT2G05760	69.433	494	150	1	2	494	27	520	0.0	716
MS.gene27812.t1	AT5G62890	59.635	493	197	1	2	492	38	530	0.0	598
MS.gene27812.t1	AT5G62890	59.635	493	197	1	2	492	38	530	0.0	598
MS.gene27812.t1	AT5G62890	59.635	493	197	1	2	492	38	530	0.0	598
MS.gene27812.t1	AT1G60030	57.404	493	208	1	2	492	44	536	0.0	596
MS.gene27812.t1	AT2G26510	58.130	492	205	1	2	492	56	547	0.0	580
MS.gene27812.t1	AT1G10540	55.242	496	217	3	2	492	42	537	0.0	563
MS.gene27812.t1	AT5G49990	56.998	493	210	1	2	492	34	526	0.0	558
MS.gene27812.t1	AT5G49990	56.998	493	210	1	2	492	34	526	0.0	558
MS.gene27812.t1	AT5G49990	56.998	493	210	1	2	492	34	526	0.0	558
MS.gene27812.t1	AT5G49990	56.998	493	210	1	2	492	34	526	0.0	558
MS.gene27812.t1	AT1G49960	56.823	491	210	1	4	492	34	524	0.0	549
MS.gene27812.t1	AT1G65550	51.200	500	233	4	2	492	42	539	0.0	523
MS.gene27812.t1	AT5G62890	58.865	423	174	0	2	424	38	460	1.18e-176	505
MS.gene27812.t1	AT2G26510	58.721	344	142	0	2	345	56	399	2.87e-135	397
MS.gene27812.t1	AT1G10540	55.908	347	150	2	2	345	42	388	2.59e-134	394
MS.gene27812.t1	AT2G26510	58.610	331	137	0	2	332	56	386	1.37e-129	383
MS.gene27812.t1	AT5G25420	42.995	414	156	4	2	409	54	393	7.21e-112	337
MS.gene27812.t1	AT2G27810	35.838	519	296	6	7	491	186	701	4.26e-101	319
MS.gene27812.t1	AT4G38050	35.962	520	297	5	4	491	189	704	1.44e-96	307
MS.gene27812.t1	AT2G27810	34.254	543	296	7	7	491	186	725	5.63e-96	306
MS.gene27812.t1	AT2G27810	37.037	405	236	4	7	393	186	589	5.30e-81	263
MS.gene27812.t1	AT2G27810	37.430	358	205	4	7	346	186	542	3.61e-69	231

Find 119 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCAGCATACAAATTATTT+TGG	0.101959	4.1:-13607845	MS.gene27812:CDS
TAATTTGTTTCAGGTAAATT+TGG	0.196029	4.1:-13606256	MS.gene27812:intron
TTCATTTCTTGTTCCTTCAA+TGG	0.226954	4.1:-13609209	MS.gene27812:CDS
GTAATTACATTAGTTGGTTT+TGG	0.246380	4.1:-13607629	MS.gene27812:CDS
TTTGATGCTGGGCACTCTTT+TGG	0.253563	4.1:-13606846	MS.gene27812:CDS
TCATAACAACCACAGTGATA+TGG	0.275276	4.1:-13607116	MS.gene27812:CDS
ATTGATTATTGCTGTGTTCT+TGG	0.284940	4.1:-13605619	MS.gene27812:CDS
ATTTCAGAGAATACACTATC+AGG	0.289187	4.1:-13605967	MS.gene27812:CDS
CCATGGCATTCCTCTATCTT+TGG	0.292772	4.1:+13605569	None:intergenic
GGACAATACTCTTGACTATA+AGG	0.306775	4.1:-13605598	MS.gene27812:CDS
AAACCAACTAATGTAATTAC+TGG	0.315074	4.1:+13607632	None:intergenic
AAGAATAGAAGATGGTATTC+AGG	0.318777	4.1:+13605655	None:intergenic
ATGATATTCTTTGCAATGTT+AGG	0.319292	4.1:-13606374	MS.gene27812:intron
CTCCTATCACTGTTGGTAAC+CGG	0.320169	4.1:+13608142	None:intergenic
GTGGGGACTTGTGTTGAAAT+TGG	0.325063	4.1:-13607474	MS.gene27812:CDS
AAATATGGGCTATTTGTTCT+AGG	0.325670	4.1:-13607813	MS.gene27812:intron
TCATTTCTTGTTCCTTCAAT+GGG	0.346165	4.1:-13609208	MS.gene27812:CDS
GTGTATTCTCTGAAATATTC+AGG	0.350685	4.1:+13605974	None:intergenic
ATCTCATTTCTTCCGCTCCT+TGG	0.363626	4.1:-13607008	MS.gene27812:intron
CTTGTTTGTAGTGTTCTCTC+AGG	0.378594	4.1:-13607436	MS.gene27812:intron
AAAAGAAGGGTCACGAATAA+TGG	0.387784	4.1:+13608089	None:intergenic
TTTGGGATTTAGTCAAATAT+GGG	0.396617	4.1:-13607827	MS.gene27812:CDS
GAGCCGTGGTATCGGTTGGC+AGG	0.401838	4.1:-13606607	MS.gene27812:CDS
GTTCCAGTAATTACATTAGT+TGG	0.413657	4.1:-13607635	MS.gene27812:CDS
TGCCAACACGGTTGCTTCCT+AGG	0.415342	4.1:+13606429	None:intergenic
GGTATCGGTTGGCAGGGAAT+TGG	0.421613	4.1:-13606600	MS.gene27812:CDS
AGGGTTATTCAAGTTTCTGC+TGG	0.424755	4.1:-13606401	MS.gene27812:CDS
GGTGCTCCTACATTTGATGC+TGG	0.427984	4.1:-13606858	MS.gene27812:CDS
TACACTATCAGGGCCTTTCA+TGG	0.429220	4.1:-13605956	MS.gene27812:CDS
TCCCAAAATAATTTGTATGC+TGG	0.431042	4.1:+13607843	None:intergenic
AGAGAGAATGTAGGACTCCT+AGG	0.436161	4.1:-13606446	MS.gene27812:CDS
CATAACAACCACAGTGATAT+GGG	0.441500	4.1:-13607115	MS.gene27812:CDS
TGTTGCAGGATGATAAAGTA+AGG	0.446203	4.1:-13608227	MS.gene27812:intron
AGGAGGATCATATGCATTCA+TGG	0.453580	4.1:-13608124	MS.gene27812:CDS
ACATCATTTCCACCCATTGA+AGG	0.466107	4.1:+13609196	None:intergenic
AACATAGTTGTAGGACAGAC+AGG	0.467914	4.1:-13607035	MS.gene27812:CDS
TCACGAATAATGGATACAAT+GGG	0.468928	4.1:+13608099	None:intergenic
TGCTCATACCAAAGCTGGAT+GGG	0.470991	4.1:-13605930	MS.gene27812:intron
TGCAAACACTGTTTGGAACC+CGG	0.473980	4.1:-13608161	MS.gene27812:CDS
AATGATAGATGACCCACAAT+TGG	0.477393	4.1:-13608064	MS.gene27812:intron
TCCTATCACTGTTGGTAACC+GGG	0.477745	4.1:+13608143	None:intergenic
GCCAACCGATACCACGGCTC+AGG	0.478834	4.1:+13606610	None:intergenic
AGAAATGTACTAACCAATTG+TGG	0.479205	4.1:+13608051	None:intergenic
GAATAGACAAGCCTAAGAAA+AGG	0.479304	4.1:+13605996	None:intergenic
GGTCCTGCTCATACCAAAGC+TGG	0.479360	4.1:-13605935	MS.gene27812:CDS
GAAACTCACCCATCCAGCTT+TGG	0.485860	4.1:+13605922	None:intergenic
GTACACAGAAACTGTTGAAC+CGG	0.486278	4.1:+13606545	None:intergenic
ACCCGGTTACCAACAGTGAT+AGG	0.489452	4.1:-13608144	MS.gene27812:CDS
ATTGAAGGAACAAGAAATGA+AGG	0.499359	4.1:+13609211	None:intergenic
GCACCCCACTCAAGAGGGTA+TGG	0.499949	4.1:+13606876	None:intergenic
ATGTAACTCAACATAGTTGT+AGG	0.501947	4.1:-13607044	MS.gene27812:CDS
TCATCTATCATTGCAAAAGA+AGG	0.502196	4.1:+13608075	None:intergenic
AAGAAGCGGTTGAGATTGAA+AGG	0.503438	4.1:+13605489	None:intergenic
AACCGGTTAGTGTTCCGAAA+AGG	0.514137	4.1:+13606562	None:intergenic
GTGCTCCTACATTTGATGCT+GGG	0.514731	4.1:-13606857	MS.gene27812:CDS
ATGAGGAACCTCTTTATCAC+TGG	0.516659	4.1:-13606025	MS.gene27812:CDS
CATGTCCTGAGCCGTGGTAT+CGG	0.518412	4.1:-13606615	MS.gene27812:CDS
AATAGACAAGCCTAAGAAAA+GGG	0.522987	4.1:+13605997	None:intergenic
TTTCTTGTTCCTTCAATGGG+TGG	0.525960	4.1:-13609205	MS.gene27812:CDS
GCAATAATCAATGCAACAGT+TGG	0.533111	4.1:+13605630	None:intergenic
AAGGACAGTGCCAAAGATAG+AGG	0.534675	4.1:-13605579	MS.gene27812:CDS
TCCTGAGCCGTGGTATCGGT+TGG	0.538613	4.1:-13606611	MS.gene27812:CDS
TATGATCCTCCTATCACTGT+TGG	0.541705	4.1:+13608135	None:intergenic
CCACGGCTCAGGACATGAGC+AGG	0.543664	4.1:+13606621	None:intergenic
CATCCAGCTTTGGTATGAGC+AGG	0.545272	4.1:+13605932	None:intergenic
GAAATGTACTAACCAATTGT+GGG	0.546919	4.1:+13608052	None:intergenic
GAGTGCCCAGCATCAAATGT+AGG	0.547055	4.1:+13606852	None:intergenic
CTGCTCATACCAAAGCTGGA+TGG	0.547379	4.1:-13605931	MS.gene27812:CDS
GTCACGAATAATGGATACAA+TGG	0.549123	4.1:+13608098	None:intergenic
CAGTGGAGAACAATACTGCT+TGG	0.550015	4.1:-13609277	MS.gene27812:CDS
CAGAAACTTGAATAACCCTT+CGG	0.550365	4.1:+13606405	None:intergenic
CTCAGGACATGAGCAGGCGG+TGG	0.550799	4.1:+13606627	None:intergenic
CAACTATGTTGAGTTACATC+CGG	0.554296	4.1:+13607048	None:intergenic
GGGCAACACCAGTGATAAAG+AGG	0.555822	4.1:+13606017	None:intergenic
GACATGAGCAGGCGGTGGTG+TGG	0.560084	4.1:+13606632	None:intergenic
TAACCCTTCGGCTGCCAACA+CGG	0.560723	4.1:+13606417	None:intergenic
ATTTGACAGAGGTTTCCCTA+TGG	0.567601	4.1:-13607603	MS.gene27812:intron
GTTCCTCATTGAATTCATGT+TGG	0.571510	4.1:+13606039	None:intergenic
GCAACCGTGTTGGCAGCCGA+AGG	0.576172	4.1:-13606421	MS.gene27812:CDS
CACTACAAACAAGATTAGCA+TGG	0.579230	4.1:+13607446	None:intergenic
CTCCTAGGAAGCAACCGTGT+TGG	0.580038	4.1:-13606431	MS.gene27812:CDS
TTTCAGAGAATACACTATCA+GGG	0.580243	4.1:-13605966	MS.gene27812:CDS
AGTTGGTGAAGAATAGAAGA+TGG	0.581474	4.1:+13605647	None:intergenic
CCTGCTCATGTCCTGAGCCG+TGG	0.582782	4.1:-13606621	MS.gene27812:CDS
AGCCGTGGTATCGGTTGGCA+GGG	0.590320	4.1:-13606606	MS.gene27812:CDS
GGTATGAGCAGGACCATGAA+AGG	0.591544	4.1:+13605943	None:intergenic
CATCTATCATTGCAAAAGAA+GGG	0.595562	4.1:+13608076	None:intergenic
TTCCCTGCCAACCGATACCA+CGG	0.595568	4.1:+13606604	None:intergenic
AGAGCAAATCTCTCTAGTAT+CGG	0.602160	4.1:+13607141	None:intergenic
CATCTTGACAGAGAGAATGT+AGG	0.602743	4.1:-13606455	MS.gene27812:intron
ACTACAAACAAGATTAGCAT+GGG	0.605867	4.1:+13607447	None:intergenic
AGATTCCATACCCTCTTGAG+TGG	0.607942	4.1:-13606881	MS.gene27812:CDS
ATGCTACAATTAGTGCACCT+TGG	0.608851	4.1:+13607866	None:intergenic
TAGGAGCACCCCACTCAAGA+GGG	0.617390	4.1:+13606871	None:intergenic
GCACATCTATTGACAGCGAG+TGG	0.619907	4.1:-13607087	MS.gene27812:CDS
GATTCCATACCCTCTTGAGT+GGG	0.620879	4.1:-13606880	MS.gene27812:CDS
GTAGGAGCACCCCACTCAAG+AGG	0.622953	4.1:+13606870	None:intergenic
AAGATAGAGGAATGCCATGG+TGG	0.624875	4.1:-13605566	MS.gene27812:CDS
TATGTTGAGTTACATCCGGC+CGG	0.624907	4.1:+13607052	None:intergenic
CAACCGTGTTGGCAGCCGAA+GGG	0.626544	4.1:-13606420	MS.gene27812:CDS
AGATAGAGGAATGCCATGGT+GGG	0.635902	4.1:-13605565	MS.gene27812:CDS
GTTGCAGGATGATAAAGTAA+GGG	0.641584	4.1:-13608226	MS.gene27812:intron
TGGAGCATACAAACACCGGC+CGG	0.643571	4.1:-13607067	MS.gene27812:CDS
CGGTTACCAACAGTGATAGG+AGG	0.653551	4.1:-13608141	MS.gene27812:CDS
GCAGGATGATAAAGTAAGGG+TGG	0.654485	4.1:-13608223	MS.gene27812:intron
AGCACAATGAGAGCAGTCCA+AGG	0.659630	4.1:-13607883	MS.gene27812:CDS
CGAGTGGAGCATACAAACAC+CGG	0.663938	4.1:-13607071	MS.gene27812:CDS
CGGCTCAGGACATGAGCAGG+CGG	0.664877	4.1:+13606624	None:intergenic
ATTCCATACCCTCTTGAGTG+GGG	0.671790	4.1:-13606879	MS.gene27812:CDS
GTTCTAAACTTAGCCCACCA+TGG	0.673056	4.1:+13605552	None:intergenic
TATTGGTACCAACCAAGGAG+CGG	0.679674	4.1:+13606996	None:intergenic
ATTACTGGAACCATTCCAAG+TGG	0.685617	4.1:+13607647	None:intergenic
CCAAAGATAGAGGAATGCCA+TGG	0.686802	4.1:-13605569	MS.gene27812:CDS
TCACCAACATGAATTCAATG+AGG	0.689628	4.1:-13606042	MS.gene27812:CDS
ACATAGTTGTAGGACAGACA+GGG	0.696081	4.1:-13607034	MS.gene27812:CDS
TCTATGATGGAGGAAAGAAG+CGG	0.698502	4.1:+13605475	None:intergenic
TTACTGGAACCATTCCAAGT+GGG	0.700944	4.1:+13607648	None:intergenic
CACGAATAATGGATACAATG+GGG	0.733637	4.1:+13608100	None:intergenic
TGCATATGCCCATATCACTG+TGG	0.777461	4.1:+13607107	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCTAAATATATATTTTTGT+TGG	-	chr4.1:13607216-13607235	MS.gene27812:intron	10.0%
!!	CTTCACAAATATATAAAATA+AGG	+	chr4.1:13606967-13606986	None:intergenic	15.0%
!!!	ATATGGTTTTCATTTTTTAT+GGG	-	chr4.1:13608252-13608271	MS.gene27812:intron	15.0%
!!	TAGTTAAAAGAATTGAAGTT+AGG	+	chr4.1:13605589-13605608	None:intergenic	20.0%
!!	TATCATTGGAAAAATTATAC+CGG	-	chr4.1:13607985-13608004	MS.gene27812:intron	20.0%
!!	TCACTAACTATAATTAATAC+AGG	+	chr4.1:13605982-13606001	None:intergenic	20.0%
!!!	CATATGGTTTTCATTTTTTA+TGG	-	chr4.1:13608251-13608270	MS.gene27812:intron	20.0%
!!!	TAATTTGTTTCAGGTAAATT+TGG	-	chr4.1:13608494-13608513	MS.gene27812:intron	20.0%
!!!	TATAATTAATACAGGTGTTT+TGG	+	chr4.1:13605974-13605993	None:intergenic	20.0%
!!!	TATTTTCTTTTTATCGTGTT+TGG	-	chr4.1:13606356-13606375	MS.gene27812:intron	20.0%
!!!	TATTTTTGATAAGATGCAAA+GGG	-	chr4.1:13606323-13606342	MS.gene27812:intron	20.0%
!!!	TGGTTTTCAACATTATATTT+TGG	-	chr4.1:13605493-13605512	MS.gene27812:CDS	20.0%
!!!	TTATTTTTGATAAGATGCAA+AGG	-	chr4.1:13606322-13606341	MS.gene27812:intron	20.0%
!!!	TTTAAGTTTAAATTAGCTTG+TGG	-	chr4.1:13607369-13607388	MS.gene27812:intron	20.0%
!!!	TTTTCTTATTCTCATTCATA+TGG	-	chr4.1:13608235-13608254	MS.gene27812:intron	20.0%
!	AAACCAACTAATGTAATTAC+TGG	+	chr4.1:13607121-13607140	None:intergenic	25.0%
!	AAATACCCATGACTATTTAT+TGG	-	chr4.1:13606162-13606181	MS.gene27812:intron	25.0%
!	AGTATATAGCAGCAAAAATA+GGG	+	chr4.1:13608544-13608563	None:intergenic	25.0%
!	ATGATATTCTTTGCAATGTT+AGG	-	chr4.1:13608376-13608395	MS.gene27812:intron	25.0%
!	ATTGATCCAAACACAATAAA+AGG	+	chr4.1:13605708-13605727	None:intergenic	25.0%
!	CAAAGAAAATATTGAGCTAT+TGG	-	chr4.1:13607813-13607832	MS.gene27812:intron	25.0%
!	CAACACATCATAATACATTA+AGG	+	chr4.1:13607552-13607571	None:intergenic	25.0%
!	CTTCATAAAATGACTATCAT+TGG	-	chr4.1:13607971-13607990	MS.gene27812:intron	25.0%
!	TCTATCCAATAAATAGTCAT+GGG	+	chr4.1:13606170-13606189	None:intergenic	25.0%
!	TGAAACAAATTATGAAACAC+AGG	+	chr4.1:13608486-13608505	None:intergenic	25.0%
!	TTGATCCAAACACAATAAAA+GGG	+	chr4.1:13605707-13605726	None:intergenic	25.0%
!!	AGCATCTATTGATAATTGTT+TGG	-	chr4.1:13608619-13608638	MS.gene27812:intron	25.0%
!!	ATCCAGCATACAAATTATTT+TGG	-	chr4.1:13606905-13606924	MS.gene27812:intron	25.0%
!!	TAATTCCCTTTTATTGTGTT+TGG	-	chr4.1:13605699-13605718	MS.gene27812:intron	25.0%
!!	TCCAGCATACAAATTATTTT+GGG	-	chr4.1:13606906-13606925	MS.gene27812:intron	25.0%
!!	TGAATTTTACACTGTTGTTA+AGG	-	chr4.1:13605902-13605921	MS.gene27812:intron	25.0%
!!	TTTGGGATTTAGTCAAATAT+GGG	-	chr4.1:13606923-13606942	MS.gene27812:intron	25.0%
!!!	ATATTTTTTTCAGCTTCTGT+AGG	-	chr4.1:13608668-13608687	MS.gene27812:intron	25.0%
!!!	ATTTTTTTTGTCTTGTGTAC+TGG	-	chr4.1:13606382-13606401	MS.gene27812:CDS	25.0%
!!!	CAACATTATATTTTGGCATT+AGG	-	chr4.1:13605500-13605519	MS.gene27812:CDS	25.0%
!!!	CTACTTTGATTTGATTTTGA+TGG	-	chr4.1:13606283-13606302	MS.gene27812:intron	25.0%
!!!	GTAATTACATTAGTTGGTTT+TGG	-	chr4.1:13607121-13607140	MS.gene27812:CDS	25.0%
!!!	TATTTTTTTCAGCTTCTGTA+GGG	-	chr4.1:13608669-13608688	MS.gene27812:intron	25.0%
!!!	TGGATCAATGTTTTAACAAT+CGG	-	chr4.1:13605719-13605738	MS.gene27812:intron	25.0%
!!!	TGTGTTTCATAATTTGTTTC+AGG	-	chr4.1:13608485-13608504	MS.gene27812:intron	25.0%
!!!	TTATTATTTTGACAGTCAAC+TGG	-	chr4.1:13608063-13608082	MS.gene27812:intron	25.0%
!!!	TTTGTCTATTGGTTTAATCA+TGG	-	chr4.1:13605830-13605849	MS.gene27812:intron	25.0%
!!!	TTTTGGGATTTAGTCAAATA+TGG	-	chr4.1:13606922-13606941	MS.gene27812:intron	25.0%
	AAAATCTGCATGAAAGGAAA+AGG	+	chr4.1:13607078-13607097	None:intergenic	30.0%
	AAACCAATAGACAAACTAGT+AGG	+	chr4.1:13605825-13605844	None:intergenic	30.0%
	AACTGGTTGAATTGGAAATT+GGG	-	chr4.1:13605773-13605792	MS.gene27812:intron	30.0%
	AAGAATAGAAGATGGTATTC+AGG	+	chr4.1:13609098-13609117	None:intergenic	30.0%
	AATAGACAAGCCTAAGAAAA+GGG	+	chr4.1:13608756-13608775	None:intergenic	30.0%
	ACTACAAACAAGATTAGCAT+GGG	+	chr4.1:13607306-13607325	None:intergenic	30.0%
	AGAAATGTACTAACCAATTG+TGG	+	chr4.1:13606702-13606721	None:intergenic	30.0%
	AGATGCTGAAGTAAATTCAA+AGG	+	chr4.1:13608606-13608625	None:intergenic	30.0%
	AGTTGAATCAGATTGAATAG+TGG	-	chr4.1:13605867-13605886	MS.gene27812:intron	30.0%
	ATGTAACTCAACATAGTTGT+AGG	-	chr4.1:13607706-13607725	MS.gene27812:intron	30.0%
	ATTGAAGGAACAAGAAATGA+AGG	+	chr4.1:13605542-13605561	None:intergenic	30.0%
	ATTGATTATTGCTGTGTTCT+TGG	-	chr4.1:13609131-13609150	MS.gene27812:intron	30.0%
	ATTTCAGAGAATACACTATC+AGG	-	chr4.1:13608783-13608802	MS.gene27812:intron	30.0%
	CAGTATATAGCAGCAAAAAT+AGG	+	chr4.1:13608545-13608564	None:intergenic	30.0%
	CATATATTGCTTATTGTTGC+AGG	-	chr4.1:13606509-13606528	MS.gene27812:intron	30.0%
	CATCTATCATTGCAAAAGAA+GGG	+	chr4.1:13606677-13606696	None:intergenic	30.0%
	CATGACTATTTATTGGATAG+AGG	-	chr4.1:13606169-13606188	MS.gene27812:intron	30.0%
	CTCTATCCAATAAATAGTCA+TGG	+	chr4.1:13606171-13606190	None:intergenic	30.0%
	GAAATGTACTAACCAATTGT+GGG	+	chr4.1:13606701-13606720	None:intergenic	30.0%
	GACAATGAAAAATACTAGCA+AGG	-	chr4.1:13606098-13606117	MS.gene27812:intron	30.0%
	GATGCTGAAGTAAATTCAAA+GGG	+	chr4.1:13608605-13608624	None:intergenic	30.0%
	GTAACAAATTGAACAGAAAG+AGG	+	chr4.1:13607505-13607524	None:intergenic	30.0%
	GTATGTTTAACAGGTTGTAA+CGG	-	chr4.1:13605796-13605815	MS.gene27812:intron	30.0%
	GTTCCAGTAATTACATTAGT+TGG	-	chr4.1:13607115-13607134	MS.gene27812:CDS	30.0%
	TATATGTCAAATAACTGGAC+TGG	-	chr4.1:13607337-13607356	MS.gene27812:intron	30.0%
	TCAGGTATATGTCAAATAAC+TGG	-	chr4.1:13607332-13607351	MS.gene27812:intron	30.0%
	TCATCTATCATTGCAAAAGA+AGG	+	chr4.1:13606678-13606697	None:intergenic	30.0%
	TCATTCATTCAAACCTATTC+AGG	+	chr4.1:13607529-13607548	None:intergenic	30.0%
	TCATTGAACTGCAAAATCAA+AGG	+	chr4.1:13609072-13609091	None:intergenic	30.0%
	TCATTTCTTGTTCCTTCAAT+GGG	-	chr4.1:13605542-13605561	MS.gene27812:CDS	30.0%
	TGAATATCAATCCTCTATCT+TGG	+	chr4.1:13606491-13606510	None:intergenic	30.0%
	TTCATTTCTTGTTCCTTCAA+TGG	-	chr4.1:13605541-13605560	MS.gene27812:CDS	30.0%
	TTTCAGAGAATACACTATCA+GGG	-	chr4.1:13608784-13608803	MS.gene27812:intron	30.0%
!	AAATATGGGCTATTTGTTCT+AGG	-	chr4.1:13606937-13606956	MS.gene27812:intron	30.0%
!	GATAATTGTTTGGTCTTCTT+TGG	-	chr4.1:13608629-13608648	MS.gene27812:intron	30.0%
!	TAACCTACTAGTTTGTCTAT+TGG	-	chr4.1:13605819-13605838	MS.gene27812:intron	30.0%
!	TCCCAAAATAATTTGTATGC+TGG	+	chr4.1:13606910-13606929	None:intergenic	30.0%
!!	GCTATATACTGTGTTTTGTT+TGG	-	chr4.1:13608554-13608573	MS.gene27812:intron	30.0%
!!	GTGTATTCTCTGAAATATTC+AGG	+	chr4.1:13608779-13608798	None:intergenic	30.0%
!!	TCAATTTGTTACACCTGAAT+AGG	-	chr4.1:13607513-13607532	MS.gene27812:intron	30.0%
!!	TCACGAATAATGGATACAAT+GGG	+	chr4.1:13606654-13606673	None:intergenic	30.0%
!!	TTAAATTAGCTTGTGGAATG+TGG	-	chr4.1:13607376-13607395	MS.gene27812:intron	30.0%
!!	TTGATTTTGATGGATGATAC+TGG	-	chr4.1:13606293-13606312	MS.gene27812:intron	30.0%
!!!	CAAACTCTACTTTTTGTTGA+AGG	-	chr4.1:13606552-13606571	MS.gene27812:CDS	30.0%
!!!	GTTTTCATTTTTTATGGGAC+AGG	-	chr4.1:13608257-13608276	MS.gene27812:intron	30.0%
!!!	TGTTTTAGTCTCATTAGTTG+AGG	-	chr4.1:13607939-13607958	MS.gene27812:intron	30.0%
	AAAAGAAGGGTCACGAATAA+TGG	+	chr4.1:13606664-13606683	None:intergenic	35.0%
	AATGATAGATGACCCACAAT+TGG	-	chr4.1:13606686-13606705	MS.gene27812:intron	35.0%
	ACTGGTTGAATTGGAAATTG+GGG	-	chr4.1:13605774-13605793	MS.gene27812:intron	35.0%
	AGAGCAAATCTCTCTAGTAT+CGG	+	chr4.1:13607612-13607631	None:intergenic	35.0%
	ATAGCAGCAAAAATAGGGAA+TGG	+	chr4.1:13608539-13608558	None:intergenic	35.0%
	ATTTCCTTCTTTCTTGCTCT+AGG	+	chr4.1:13607464-13607483	None:intergenic	35.0%
	CAACTATGTTGAGTTACATC+CGG	+	chr4.1:13607705-13607724	None:intergenic	35.0%
	CAACTGGTTGAATTGGAAAT+TGG	-	chr4.1:13605772-13605791	MS.gene27812:intron	35.0%
	CACTACAAACAAGATTAGCA+TGG	+	chr4.1:13607307-13607326	None:intergenic	35.0%
	CAGAAACTTGAATAACCCTT+CGG	+	chr4.1:13608348-13608367	None:intergenic	35.0%
	CAGTGTTGTACTATACCATA+GGG	+	chr4.1:13607165-13607184	None:intergenic	35.0%
	CATAACAACCACAGTGATAT+GGG	-	chr4.1:13607635-13607654	MS.gene27812:CDS	35.0%
	CTCTATGCAAATCTCTACTT+AGG	-	chr4.1:13605666-13605685	MS.gene27812:CDS	35.0%
	GAAATTGGGGTATGTTTAAC+AGG	-	chr4.1:13605787-13605806	MS.gene27812:intron	35.0%
	GAATAGACAAGCCTAAGAAA+AGG	+	chr4.1:13608757-13608776	None:intergenic	35.0%
	GCAATAATCAATGCAACAGT+TGG	+	chr4.1:13609123-13609142	None:intergenic	35.0%
	GGACAATACTCTTGACTATA+AGG	-	chr4.1:13609152-13609171	MS.gene27812:intron	35.0%
	GTTCCTCATTGAATTCATGT+TGG	+	chr4.1:13608714-13608733	None:intergenic	35.0%
	GTTGCAGGATGATAAAGTAA+GGG	-	chr4.1:13606524-13606543	MS.gene27812:intron	35.0%
	TAGGTGAGTGGCAAATTATA+GGG	-	chr4.1:13608395-13608414	MS.gene27812:intron	35.0%
	TCACCAACATGAATTCAATG+AGG	-	chr4.1:13608708-13608727	MS.gene27812:intron	35.0%
	TCAGTGTTGTACTATACCAT+AGG	+	chr4.1:13607166-13607185	None:intergenic	35.0%
	TCATAACAACCACAGTGATA+TGG	-	chr4.1:13607634-13607653	MS.gene27812:CDS	35.0%
	TGTTGCAGGATGATAAAGTA+AGG	-	chr4.1:13606523-13606542	MS.gene27812:intron	35.0%
	TTAGGTGAGTGGCAAATTAT+AGG	-	chr4.1:13608394-13608413	MS.gene27812:intron	35.0%
	TTCAGTTCAACTGGTTGAAT+TGG	-	chr4.1:13605765-13605784	MS.gene27812:intron	35.0%
	TTGTCACATGCTAAATGAAG+TGG	-	chr4.1:13606408-13606427	MS.gene27812:CDS	35.0%
!	ACACTTTTGCAAACACTGTT+TGG	-	chr4.1:13606582-13606601	MS.gene27812:CDS	35.0%
!	ATTTTGCTGAATGGCCTTTT+CGG	-	chr4.1:13608174-13608193	MS.gene27812:CDS	35.0%
!	GGAATTGGAATTTTGCTGAA+TGG	-	chr4.1:13608165-13608184	MS.gene27812:CDS	35.0%
!!	AGTTGGTGAAGAATAGAAGA+TGG	+	chr4.1:13609106-13609125	None:intergenic	35.0%
!!	CACGAATAATGGATACAATG+GGG	+	chr4.1:13606653-13606672	None:intergenic	35.0%
!!	GTCACGAATAATGGATACAA+TGG	+	chr4.1:13606655-13606674	None:intergenic	35.0%
!!	GTCTATTGGTTTAATCATGG+TGG	-	chr4.1:13605833-13605852	MS.gene27812:intron	35.0%
!!!	GGTTTTGGATTATTTGACAG+AGG	-	chr4.1:13607136-13607155	MS.gene27812:CDS	35.0%
!!!	TGTGTTTTGTTTGGTCTTGT+AGG	-	chr4.1:13608563-13608582	MS.gene27812:intron	35.0%
	AAAGCCTAGAGCAAGAAAGA+AGG	-	chr4.1:13607457-13607476	MS.gene27812:CDS	40.0%
	AACATAGTTGTAGGACAGAC+AGG	-	chr4.1:13607715-13607734	MS.gene27812:intron	40.0%
	AAGAAGCGGTTGAGATTGAA+AGG	+	chr4.1:13609264-13609283	None:intergenic	40.0%
	ACATAGTTGTAGGACAGACA+GGG	-	chr4.1:13607716-13607735	MS.gene27812:intron	40.0%
	ACATCATTTCCACCCATTGA+AGG	+	chr4.1:13605557-13605576	None:intergenic	40.0%
	AGGAGGATCATATGCATTCA+TGG	-	chr4.1:13606626-13606645	MS.gene27812:CDS	40.0%
	ATCATATCTCATCTCGTCTC+AGG	-	chr4.1:13608875-13608894	MS.gene27812:intron	40.0%
	ATGAGGAACCTCTTTATCAC+TGG	-	chr4.1:13608725-13608744	MS.gene27812:intron	40.0%
	ATGCTACAATTAGTGCACCT+TGG	+	chr4.1:13606887-13606906	None:intergenic	40.0%
	ATTACTGGAACCATTCCAAG+TGG	+	chr4.1:13607106-13607125	None:intergenic	40.0%
	ATTTGACAGAGGTTTCCCTA+TGG	-	chr4.1:13607147-13607166	MS.gene27812:CDS	40.0%
	CACATGCTAAATGAAGTGGT+GGG	-	chr4.1:13606412-13606431	MS.gene27812:CDS	40.0%
	CATCTTGACAGAGAGAATGT+AGG	-	chr4.1:13608295-13608314	MS.gene27812:intron	40.0%
	CCTCAAACTTACCAAGATAG+AGG	-	chr4.1:13606477-13606496	MS.gene27812:intron	40.0%
	CCTCTATCTTGGTAAGTTTG+AGG	+	chr4.1:13606480-13606499	None:intergenic	40.0%
	CTTGTTTGTAGTGTTCTCTC+AGG	-	chr4.1:13607314-13607333	MS.gene27812:intron	40.0%
	GAATACAAGTACAAGTCACG+TGG	+	chr4.1:13607191-13607210	None:intergenic	40.0%
	GAGATACACAAACATGGAAG+AGG	+	chr4.1:13606835-13606854	None:intergenic	40.0%
	GGCTGAAAAATCTGCATGAA+AGG	+	chr4.1:13607084-13607103	None:intergenic	40.0%
	GTACACAGAAACTGTTGAAC+CGG	+	chr4.1:13608208-13608227	None:intergenic	40.0%
	TATGATCCTCCTATCACTGT+TGG	+	chr4.1:13606618-13606637	None:intergenic	40.0%
	TCACATGCTAAATGAAGTGG+TGG	-	chr4.1:13606411-13606430	MS.gene27812:CDS	40.0%
	TCATATCTCATCTCGTCTCA+GGG	-	chr4.1:13608876-13608895	MS.gene27812:intron	40.0%
	TCATTGTGCTAAGAAACCTC+TGG	+	chr4.1:13606857-13606876	None:intergenic	40.0%
	TCTCCCAAAAGAGGAAAATC+TGG	-	chr4.1:13606435-13606454	MS.gene27812:CDS	40.0%
	TCTTTGCAATGTTAGGTGAG+TGG	-	chr4.1:13608383-13608402	MS.gene27812:intron	40.0%
	TGTGTGTTACGTAAGCATAC+CGG	+	chr4.1:13608007-13608026	None:intergenic	40.0%
	TTACTGGAACCATTCCAAGT+GGG	+	chr4.1:13607105-13607124	None:intergenic	40.0%
	TTCGACAACAACGATGCTAT+TGG	+	chr4.1:13607774-13607793	None:intergenic	40.0%
	TTTCTTGTTCCTTCAATGGG+TGG	-	chr4.1:13605545-13605564	MS.gene27812:CDS	40.0%
!	AGGGTTATTCAAGTTTCTGC+TGG	-	chr4.1:13608349-13608368	MS.gene27812:intron	40.0%
!	CCACCAGATTTTCCTCTTTT+GGG	+	chr4.1:13606441-13606460	None:intergenic	40.0%
!	TCCACCAGATTTTCCTCTTT+TGG	+	chr4.1:13606442-13606461	None:intergenic	40.0%
!!	ATTTTTCAGCCCACTTGGAA+TGG	-	chr4.1:13607093-13607112	MS.gene27812:CDS	40.0%
!!	CATTGACTCTTGACACATGT+TGG	-	chr4.1:13607836-13607855	MS.gene27812:CDS	40.0%
	AACCGGTTAGTGTTCCGAAA+AGG	+	chr4.1:13608191-13608210	None:intergenic	45.0%
	AAGATAGAGGAATGCCATGG+TGG	-	chr4.1:13609184-13609203	MS.gene27812:intron	45.0%
	AAGGACAGTGCCAAAGATAG+AGG	-	chr4.1:13609171-13609190	MS.gene27812:intron	45.0%
	ACCAGTCAGTTCAGTTCAAC+TGG	-	chr4.1:13605756-13605775	MS.gene27812:intron	45.0%
	ACCAGTTGAACTGAACTGAC+TGG	+	chr4.1:13605760-13605779	None:intergenic	45.0%
	AGAGAGAATGTAGGACTCCT+AGG	-	chr4.1:13608304-13608323	MS.gene27812:intron	45.0%
	AGATAGAGGAATGCCATGGT+GGG	-	chr4.1:13609185-13609204	MS.gene27812:intron	45.0%
	AGATTCCATACCCTCTTGAG+TGG	-	chr4.1:13607869-13607888	MS.gene27812:CDS	45.0%
	ATCTCATTTCTTCCGCTCCT+TGG	-	chr4.1:13607742-13607761	MS.gene27812:intron	45.0%
	ATGCTGTTCTGTCCTTTCTC+AGG	-	chr4.1:13607254-13607273	MS.gene27812:intron	45.0%
	ATTCCATACCCTCTTGAGTG+GGG	-	chr4.1:13607871-13607890	MS.gene27812:CDS	45.0%
	CATATCTCATCTCGTCTCAG+GGG	-	chr4.1:13608877-13608896	MS.gene27812:intron	45.0%
	CCAAAGATAGAGGAATGCCA+TGG	-	chr4.1:13609181-13609200	MS.gene27812:intron	45.0%
	CCATGGCATTCCTCTATCTT+TGG	+	chr4.1:13609184-13609203	None:intergenic	45.0%
	CCATGTTTGTGTATCTCCAG+AGG	-	chr4.1:13606838-13606857	MS.gene27812:CDS	45.0%
	CCCAAAAGAGGAAAATCTGG+TGG	-	chr4.1:13606438-13606457	MS.gene27812:CDS	45.0%
	GATGCTATTGGTACCAACCA+AGG	+	chr4.1:13607762-13607781	None:intergenic	45.0%
	GATTCCATACCCTCTTGAGT+GGG	-	chr4.1:13607870-13607889	MS.gene27812:CDS	45.0%
	GCAGGATGATAAAGTAAGGG+TGG	-	chr4.1:13606527-13606546	MS.gene27812:intron	45.0%
	GCTTCTTTCCTCCATCATAG+TGG	-	chr4.1:13609277-13609296	MS.gene27812:CDS	45.0%
	GTGCTCCTACATTTGATGCT+GGG	-	chr4.1:13607893-13607912	MS.gene27812:CDS	45.0%
	GTGGGGACTTGTGTTGAAAT+TGG	-	chr4.1:13607276-13607295	MS.gene27812:intron	45.0%
	TACACTATCAGGGCCTTTCA+TGG	-	chr4.1:13608794-13608813	MS.gene27812:intron	45.0%
	TATGTTGAGTTACATCCGGC+CGG	+	chr4.1:13607701-13607720	None:intergenic	45.0%
	TATTGGTACCAACCAAGGAG+CGG	+	chr4.1:13607757-13607776	None:intergenic	45.0%
	TGCATATGCCCATATCACTG+TGG	+	chr4.1:13607646-13607665	None:intergenic	45.0%
	TGCTCATACCAAAGCTGGAT+GGG	-	chr4.1:13608820-13608839	MS.gene27812:intron	45.0%
	TGTTCTGTCCTTTCTCAGGT+GGG	-	chr4.1:13607258-13607277	MS.gene27812:intron	45.0%
!	CCTCTGGAGATACACAAACA+TGG	+	chr4.1:13606841-13606860	None:intergenic	45.0%
!	GTTCTAAACTTAGCCCACCA+TGG	+	chr4.1:13609201-13609220	None:intergenic	45.0%
!	TGCAAACACTGTTTGGAACC+CGG	-	chr4.1:13606589-13606608	MS.gene27812:CDS	45.0%
!	TTTGATGCTGGGCACTCTTT+TGG	-	chr4.1:13607904-13607923	MS.gene27812:intron	45.0%
!!	CTCCTATCACTGTTGGTAAC+CGG	+	chr4.1:13606611-13606630	None:intergenic	45.0%
!!	TCCTATCACTGTTGGTAACC+GGG	+	chr4.1:13606610-13606629	None:intergenic	45.0%
!!	TGCAGATTTTTCAGCCCACT+TGG	-	chr4.1:13607088-13607107	MS.gene27812:CDS	45.0%
!!!	ACTGGTGTTGCCCTTTTCTT+AGG	-	chr4.1:13608743-13608762	MS.gene27812:intron	45.0%
!!!	TGGGCACTCTTTTGGAATGA+TGG	-	chr4.1:13607912-13607931	MS.gene27812:intron	45.0%
	ACCCGGTTACCAACAGTGAT+AGG	-	chr4.1:13606606-13606625	MS.gene27812:CDS	50.0%
	AGCACAATGAGAGCAGTCCA+AGG	-	chr4.1:13606867-13606886	MS.gene27812:CDS	50.0%
	CATTTCTTCCGCTCCTTGGT+TGG	-	chr4.1:13607746-13607765	MS.gene27812:intron	50.0%
	CGAGTGGAGCATACAAACAC+CGG	-	chr4.1:13607679-13607698	MS.gene27812:intron	50.0%
	CGGTTACCAACAGTGATAGG+AGG	-	chr4.1:13606609-13606628	MS.gene27812:CDS	50.0%
	CTCTTGACACATGTTGGTGC+AGG	-	chr4.1:13607842-13607861	MS.gene27812:CDS	50.0%
	CTGCTCATACCAAAGCTGGA+TGG	-	chr4.1:13608819-13608838	MS.gene27812:intron	50.0%
	CTGTTCTGTCCTTTCTCAGG+TGG	-	chr4.1:13607257-13607276	MS.gene27812:intron	50.0%
	GAAACTCACCCATCCAGCTT+TGG	+	chr4.1:13608831-13608850	None:intergenic	50.0%
	GAGTGCCCAGCATCAAATGT+AGG	+	chr4.1:13607901-13607920	None:intergenic	50.0%
	GCACATCTATTGACAGCGAG+TGG	-	chr4.1:13607663-13607682	MS.gene27812:intron	50.0%
	GGGCAACACCAGTGATAAAG+AGG	+	chr4.1:13608736-13608755	None:intergenic	50.0%
	GGTATGAGCAGGACCATGAA+AGG	+	chr4.1:13608810-13608829	None:intergenic	50.0%
	GGTGCTCCTACATTTGATGC+TGG	-	chr4.1:13607892-13607911	MS.gene27812:CDS	50.0%
	GTTCTGTCCTTTCTCAGGTG+GGG	-	chr4.1:13607259-13607278	MS.gene27812:intron	50.0%
!	CATCCAGCTTTGGTATGAGC+AGG	+	chr4.1:13608821-13608840	None:intergenic	50.0%
!	GGCCTTTTCGGAACACTAAC+CGG	-	chr4.1:13608186-13608205	MS.gene27812:CDS	50.0%
!!	ACTGACTGGTTCGACTCGTT+CGG	+	chr4.1:13605746-13605765	None:intergenic	50.0%
	CACAAGTCCCCACCTGAGAA+AGG	+	chr4.1:13607269-13607288	None:intergenic	55.0%
	CATGTCCTGAGCCGTGGTAT+CGG	-	chr4.1:13608135-13608154	MS.gene27812:CDS	55.0%
	CTCCTAGGAAGCAACCGTGT+TGG	-	chr4.1:13608319-13608338	MS.gene27812:intron	55.0%
	GGTCCTGCTCATACCAAAGC+TGG	-	chr4.1:13608815-13608834	MS.gene27812:intron	55.0%
	TAACCCTTCGGCTGCCAACA+CGG	+	chr4.1:13608336-13608355	None:intergenic	55.0%
	TGCCAACACGGTTGCTTCCT+AGG	+	chr4.1:13608324-13608343	None:intergenic	55.0%
	TGGAGCATACAAACACCGGC+CGG	-	chr4.1:13607683-13607702	MS.gene27812:intron	55.0%
	TTCCCTGCCAACCGATACCA+CGG	+	chr4.1:13608149-13608168	None:intergenic	55.0%
!!	AGTGGTGGGTCTCCCAAAAG+AGG	-	chr4.1:13606426-13606445	MS.gene27812:CDS	55.0%
!!	GGTATCGGTTGGCAGGGAAT+TGG	-	chr4.1:13608150-13608169	MS.gene27812:CDS	55.0%
!!	TAGGAGCACCCCACTCAAGA+GGG	+	chr4.1:13607882-13607901	None:intergenic	55.0%
	GCACCCCACTCAAGAGGGTA+TGG	+	chr4.1:13607877-13607896	None:intergenic	60.0%
!	CAACCGTGTTGGCAGCCGAA+GGG	-	chr4.1:13608330-13608349	MS.gene27812:intron	60.0%
!!	AGCCGTGGTATCGGTTGGCA+GGG	-	chr4.1:13608144-13608163	MS.gene27812:CDS	60.0%
!!	GTAGGAGCACCCCACTCAAG+AGG	+	chr4.1:13607883-13607902	None:intergenic	60.0%
!!	TCCTGAGCCGTGGTATCGGT+TGG	-	chr4.1:13608139-13608158	MS.gene27812:CDS	60.0%
	CCACGGCTCAGGACATGAGC+AGG	+	chr4.1:13608132-13608151	None:intergenic	65.0%
	CCTGCTCATGTCCTGAGCCG+TGG	-	chr4.1:13608129-13608148	MS.gene27812:CDS	65.0%
	CGGCTCAGGACATGAGCAGG+CGG	+	chr4.1:13608129-13608148	None:intergenic	65.0%
	CTCAGGACATGAGCAGGCGG+TGG	+	chr4.1:13608126-13608145	None:intergenic	65.0%
	GACATGAGCAGGCGGTGGTG+TGG	+	chr4.1:13608121-13608140	None:intergenic	65.0%
	GCCAACCGATACCACGGCTC+AGG	+	chr4.1:13608143-13608162	None:intergenic	65.0%
!	GCAACCGTGTTGGCAGCCGA+AGG	-	chr4.1:13608329-13608348	MS.gene27812:intron	65.0%
!!	GAGCCGTGGTATCGGTTGGC+AGG	-	chr4.1:13608143-13608162	MS.gene27812:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	13605476	13609296	13605476	ID=MS.gene27812
chr4.1	mRNA	13605476	13609296	13605476	ID=MS.gene27812.t1;Parent=MS.gene27812
chr4.1	exon	13609198	13609296	13609198	ID=MS.gene27812.t1.exon1;Parent=MS.gene27812.t1
chr4.1	CDS	13609198	13609296	13609198	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13608065	13608241	13608065	ID=MS.gene27812.t1.exon2;Parent=MS.gene27812.t1
chr4.1	CDS	13608065	13608241	13608065	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13607814	13607914	13607814	ID=MS.gene27812.t1.exon3;Parent=MS.gene27812.t1
chr4.1	CDS	13607814	13607914	13607814	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13607604	13607679	13607604	ID=MS.gene27812.t1.exon4;Parent=MS.gene27812.t1
chr4.1	CDS	13607604	13607679	13607604	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13607437	13607496	13607437	ID=MS.gene27812.t1.exon5;Parent=MS.gene27812.t1
chr4.1	CDS	13607437	13607496	13607437	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13607009	13607193	13607009	ID=MS.gene27812.t1.exon6;Parent=MS.gene27812.t1
chr4.1	CDS	13607009	13607193	13607009	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13606812	13606908	13606812	ID=MS.gene27812.t1.exon7;Parent=MS.gene27812.t1
chr4.1	CDS	13606812	13606908	13606812	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13606549	13606694	13606549	ID=MS.gene27812.t1.exon8;Parent=MS.gene27812.t1
chr4.1	CDS	13606549	13606694	13606549	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13606375	13606466	13606375	ID=MS.gene27812.t1.exon9;Parent=MS.gene27812.t1
chr4.1	CDS	13606375	13606466	13606375	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13606188	13606265	13606188	ID=MS.gene27812.t1.exon10;Parent=MS.gene27812.t1
chr4.1	CDS	13606188	13606265	13606188	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13605931	13606091	13605931	ID=MS.gene27812.t1.exon11;Parent=MS.gene27812.t1
chr4.1	CDS	13605931	13606091	13605931	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1
chr4.1	exon	13605476	13605688	13605476	ID=MS.gene27812.t1.exon12;Parent=MS.gene27812.t1
chr4.1	CDS	13605476	13605688	13605476	ID=cds.MS.gene27812.t1;Parent=MS.gene27812.t1

Gene Sequence

>MS.gene27812

TGGAGAACAATACTGCTTGGTTTTCAACATTATATTTTGGCATTAGGAACTGCAGTTATGATTCCTTCATTTCTTGTTCCTTCAATGGGTGGAAATGATGTAAGCTATTTCCTAACTTCAATTCTTTTAACTACTACTTGAGTTTATTTATTATTTTGATTGATTGATGAAAATGAAATTATTTAATGTACTCTATGCAAATCTCTACTTAGGAAATTTTTCTTAATTCCCTTTTATTGTGTTTGGATCAATGTTTTAACAATCGGACCGAACGAGTCGAACCAGTCAGTTCAGTTCAACTGGTTGAATTGGAAATTGGGGTATGTTTAACAGGTTGTAACGGTAACCTACTAGTTTGTCTATTGGTTTAATCATGGTGGTATAACATTCTAGTTGAATCAGATTGAATAGTGGTTGATTTTCTATTGAATTTTACACTGTTGTTAAGGAAATTATTAGTAATGTTGATTTTAATATTAAAATGTGTTTTATTTACCAAAACACCTGTATTAATTATAGTTAGTGAAATAATTATATGATTGAAATATAATAAACAAGAGTATATTAGTGAAAACGAATAATAAATGTTGTATTGACATTATAAAATAGACAAATTATTTGCGACAATGAAAAATACTAGCAAGGATGACACTTAAAATGAGACGAAAAGAGTATTGTTGTGTCTCAAATACCCATGACTATTTATTGGATAGAGGAACATAAGTAGAATGAGATAATAAATAGTTAAGTATATGATTGAAAAAAGAAAATAATTTGATCAAATGCAACAAAATTTATTGTGAGTAACTACTTTGATTTGATTTTGATGGATGATACTGGTGAATATTATTTTTGATAAGATGCAAAGGGTAAAGTTTCTTATTTTCTTTTTATCGTGTTTGGACTATTTTTTTTGTCTTGTGTACTGGATTTTGTCACATGCTAAATGAAGTGGTGGGTCTCCCAAAAGAGGAAAATCTGGTGGAAGTGAAATGAATTTGCCTCAAACTTACCAAGATAGAGGATTGATATTCATATATTGCTTATTGTTGCAGGATGATAAAGTAAGGGTGGTACAAACTCTACTTTTTGTTGAAGGAATTAATACACTTTTGCAAACACTGTTTGGAACCCGGTTACCAACAGTGATAGGAGGATCATATGCATTCATGGTCCCCATTGTATCCATTATTCGTGACCCTTCTTTTGCAATGATAGATGACCCACAATTGGTTAGTACATTTCTCTAATCTCTCTTTAATGATATTGTACTGAATTACTTTACAAAATGACATTCATATTTGCTTTAATTAGTTTAACATGTTTGAATTTCACTTATTATAGTTTAAGCTAATGCCTCTTCCATGTTTGTGTATCTCCAGAGGTTTCTTAGCACAATGAGAGCAGTCCAAGGTGCACTAATTGTAGCATCCAGCATACAAATTATTTTGGGATTTAGTCAAATATGGGCTATTTGTTCTAGGTAAGCCTTATTTTATATATTTGTGAAGATTATATACAAATTATGTACTTTTGTAAGACAAAGAGTGAAGATTATATAAATCTATATAGTACTATCAAATATCAAATAATGTATGTCCTTTTCCTTTCATGCAGATTTTTCAGCCCACTTGGAATGGTTCCAGTAATTACATTAGTTGGTTTTGGATTATTTGACAGAGGTTTCCCTATGGTATAGTACAACACTGAAACCACGTGACTTGTACTTGTATTCAGAATATCTAAATATATATTTTTGTTGGATCATATTGCTCAACATGCTGTTCTGTCCTTTCTCAGGTGGGGACTTGTGTTGAAATTGGAATTCCCATGCTAATCTTGTTTGTAGTGTTCTCTCAGGTATATGTCAAATAACTGGACTGGATTAGAAAGTTTAAGTTTAAATTAGCTTGTGGAATGTGGCATTGTAAAATTTGTTTTGTCAATACAATATAGAAAATTACGAAGCTTATTGATTTTGAAAGCCTAGAGCAAGAAAGAAGGAAATAGTCTTTTAATTGTTTCACCTCTTTCTGTTCAATTTGTTACACCTGAATAGGTTTGAATGAATGACCTTAATGTATTATGATGTGTTGTATGCAGTACTTGAAAAATTTTCAGACAAGACAAGTACCGATACTAGAGAGATTTGCTCTACTCATAACAACCACAGTGATATGGGCATATGCACATCTATTGACAGCGAGTGGAGCATACAAACACCGGCCGGATGTAACTCAACATAGTTGTAGGACAGACAGGGCTAATCTCATTTCTTCCGCTCCTTGGTTGGTACCAATAGCATCGTTGTTGTCGAATATTTGCATATCAATGTAGCAAAGAAAATATTGAGCTATTGGCATTGACTCTTGACACATGTTGGTGCAGGATAAAGATTCCATACCCTCTTGAGTGGGGTGCTCCTACATTTGATGCTGGGCACTCTTTTGGAATGATGGCTGCTGTTTTAGTCTCATTAGTTGAGGTAATTTCATCTTCATAAAATGACTATCATTGGAAAAATTATACCGGTATGCTTACGTAACACACAAACTCGTTTATATTTTTGACTAACGCTTATCGCTGTTTATTATTTTGACAGTCAACTGGAGCATTCAAAGCTGCATCGCGTCTAGCAAGTGCCACACCACCGCCTGCTCATGTCCTGAGCCGTGGTATCGGTTGGCAGGGAATTGGAATTTTGCTGAATGGCCTTTTCGGAACACTAACCGGTTCAACAGTTTCTGTGTACGTGCTATTTTTCTTATTCTCATTCATATGGTTTTCATTTTTTATGGGACAGGAATTGATTCTTTTAGCATCTTGACAGAGAGAATGTAGGACTCCTAGGAAGCAACCGTGTTGGCAGCCGAAGGGTTATTCAAGTTTCTGCTGGATTTATGATATTCTTTGCAATGTTAGGTGAGTGGCAAATTATAGGGTGATGAAAATGTGTAAATTAATTAGTTCTGATTCGAATTTCATAATTTCACTTTAAAGATCATAACCTGTGTTTCATAATTTGTTTCAGGTAAATTTGGAGCTTTATTTGCATCAATACCATTCCCTATTTTTGCTGCTATATACTGTGTTTTGTTTGGTCTTGTAGGTAAGTAGTACAACTAACCCTTTGAATTTACTTCAGCATCTATTGATAATTGTTTGGTCTTCTTTGGTCTTATGATAATTGTTATATTTTTTTCAGCTTCTGTAGGGCTATCATTTTTGCAATTCACCAACATGAATTCAATGAGGAACCTCTTTATCACTGGTGTTGCCCTTTTCTTAGGCTTGTCTATTCCTGAATATTTCAGAGAATACACTATCAGGGCCTTTCATGGTCCTGCTCATACCAAAGCTGGATGGGTGAGTTTCTTGTTTATTTTATACTGTTTCACTATCATATCTCATCTCGTCTCAGGGGATAGAATGCGCGAAGCAGAAGTGTGAACTTTCAACACGTGTCATTATTTATTAAGAGAATAATTTCTTTAAGAATAAATTAGTAGATTGTGTTACTTGCAACTGAGCATGATTCAATATTTTTCTTAACATATGTTAAATAAGTTTTATTGATGAAAATATAAGTTTTTCCTTTGATTTTGCAGTTCAATGACTTCCTGAATACCATCTTCTATTCTTCACCAACTGTTGCATTGATTATTGCTGTGTTCTTGGACAATACTCTTGACTATAAGGACAGTGCCAAAGATAGAGGAATGCCATGGTGGGCTAAGTTTAGAACATTTAAAGCAGATAGCAGAAATGAAGAGTTCTACTCTCTTCCTTTCAATCTCAACCGCTTCTTTCCTCCATCATAG

Protein sequence

>MS.gene27812.t1

WRTILLGFQHYILALGTAVMIPSFLVPSMGGNDDDKVRVVQTLLFVEGINTLLQTLFGTRLPTVIGGSYAFMVPIVSIIRDPSFAMIDDPQLRFLSTMRAVQGALIVASSIQIILGFSQIWAICSRFFSPLGMVPVITLVGFGLFDRGFPMVGTCVEIGIPMLILFVVFSQYLKNFQTRQVPILERFALLITTTVIWAYAHLLTASGAYKHRPDVTQHSCRTDRANLISSAPWIKIPYPLEWGAPTFDAGHSFGMMAAVLVSLVESTGAFKAASRLASATPPPAHVLSRGIGWQGIGILLNGLFGTLTGSTVSVENVGLLGSNRVGSRRVIQVSAGFMIFFAMLGKFGALFASIPFPIFAAIYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSIPEYFREYTIRAFHGPAHTKAGWFNDFLNTIFYSSPTVALIIAVFLDNTLDYKDSAKDRGMPWWAKFRTFKADSRNEEFYSLPFNLNRFFPPS