AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072153


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072153.t1 MTR_8g015560 96.203 79 3 0 1 79 1 79 2.49e-49 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTTGAGGTGGTGTCTA+TGG 0.235479 8.1:+82026459 MS.gene072153:CDS
CGTCAAGTTAGTGCTCGTTT+TGG 0.356065 8.1:+82026568 MS.gene072153:CDS
AGGAAAGAAGCTGAAAGAAA+TGG 0.356123 8.1:+82026486 MS.gene072153:CDS
CTCCTTCTTTAATTTGTGAA+TGG 0.374793 8.1:-82026398 None:intergenic
TGAAAGGTGAGATCAAGAAA+TGG 0.464102 8.1:+82026521 MS.gene072153:CDS
GAAAGGTGAGATCAAGAAAT+GGG 0.482363 8.1:+82026522 MS.gene072153:CDS
TACCATTCACAAATTAAAGA+AGG 0.518100 8.1:+82026396 MS.gene072153:CDS
ATGGATTCAAATAAGTTGAA+AGG 0.552282 8.1:+82026505 MS.gene072153:CDS
GAGGTGGTGTCTATGGAGGA+AGG 0.559344 8.1:+82026466 MS.gene072153:CDS
TGAGATCAAGAAATGGGCTA+AGG 0.607413 8.1:+82026528 MS.gene072153:CDS
AGTTGAGGTGGTGTCTATGG+AGG 0.609297 8.1:+82026462 MS.gene072153:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATCTCTATCTTTTAA+TGG + chr8.1:82026367-82026386 MS.gene072153:CDS 20.0%
! TACCATTCACAAATTAAAGA+AGG + chr8.1:82026396-82026415 MS.gene072153:CDS 25.0%
!! AAATCTCTATCTTTTAATGG+TGG + chr8.1:82026370-82026389 MS.gene072153:CDS 25.0%
!! ATGGATTCAAATAAGTTGAA+AGG + chr8.1:82026505-82026524 MS.gene072153:CDS 25.0%
!! CTCCTTCTTTAATTTGTGAA+TGG - chr8.1:82026401-82026420 None:intergenic 30.0%
AGGAAAGAAGCTGAAAGAAA+TGG + chr8.1:82026486-82026505 MS.gene072153:CDS 35.0%
GAAAGGTGAGATCAAGAAAT+GGG + chr8.1:82026522-82026541 MS.gene072153:CDS 35.0%
TGAAAGGTGAGATCAAGAAA+TGG + chr8.1:82026521-82026540 MS.gene072153:CDS 35.0%
!! TTGTTTTTCTTCAGTTGAGG+TGG + chr8.1:82026450-82026469 MS.gene072153:CDS 35.0%
!!! TCGTTGTTTTTCTTCAGTTG+AGG + chr8.1:82026447-82026466 MS.gene072153:CDS 35.0%
!! TGAGATCAAGAAATGGGCTA+AGG + chr8.1:82026528-82026547 MS.gene072153:CDS 40.0%
TTCAGTTGAGGTGGTGTCTA+TGG + chr8.1:82026459-82026478 MS.gene072153:CDS 45.0%
! CGTCAAGTTAGTGCTCGTTT+TGG + chr8.1:82026568-82026587 MS.gene072153:CDS 45.0%
AGTTGAGGTGGTGTCTATGG+AGG + chr8.1:82026462-82026481 MS.gene072153:CDS 50.0%
GAGGTGGTGTCTATGGAGGA+AGG + chr8.1:82026466-82026485 MS.gene072153:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 82026358 82026597 82026358 ID=MS.gene072153
chr8.1 mRNA 82026358 82026597 82026358 ID=MS.gene072153.t1;Parent=MS.gene072153
chr8.1 exon 82026358 82026597 82026358 ID=MS.gene072153.t1.exon1;Parent=MS.gene072153.t1
chr8.1 CDS 82026358 82026597 82026358 ID=cds.MS.gene072153.t1;Parent=MS.gene072153.t1
Gene Sequence

>MS.gene072153

ATGTCTGTGAAGAAATCTCTATCTTTTAATGGTGGAAGTACCATTCACAAATTAAAGAAGGAGAAATCTACAAAACGAAGACGTTTTGATCGTTGTTTTTCTTCAGTTGAGGTGGTGTCTATGGAGGAAGGAAAGAAGCTGAAAGAAATGGATTCAAATAAGTTGAAAGGTGAGATCAAGAAATGGGCTAAGGCTGTTGTTGCATATGCTCGTCAAGTTAGTGCTCGTTTTGGAAGCTAG

Protein sequence

>MS.gene072153.t1

MSVKKSLSFNGGSTIHKLKKEKSTKRRRFDRCFSSVEVVSMEEGKKLKEMDSNKLKGEIKKWAKAVVAYARQVSARFGS