AlfalfaGEDB | MS.gene36571

M. sativa cultivar XinJiangDaYe / MS.gene36571

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36571.t1	KEH18277.1	96.2	79	3	0	1	79	1	79	2.90E-31	144.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36571.t1	A0A072TLH5	96.2	79	3	0	1	79	1	79	2.1e-31	144.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene36571	0.835781	1.37E-56	-1.69E-46
MS.gene04919	MS.gene36571	0.820357	7.16E-53	-1.69E-46
MS.gene04920	MS.gene36571	0.839376	1.64E-57	-1.69E-46
MS.gene049254	MS.gene36571	0.821745	3.43E-53	-1.69E-46
MS.gene049283	MS.gene36571	0.886036	4.85E-72	-1.69E-46
MS.gene049385	MS.gene36571	-0.804498	2.10E-49	-1.69E-46
MS.gene049419	MS.gene36571	-0.86384	1.87E-64	-1.69E-46
MS.gene049452	MS.gene36571	0.833286	5.82E-56	-1.69E-46
MS.gene049499	MS.gene36571	0.870136	1.83E-66	-1.69E-46
MS.gene049983	MS.gene36571	0.866207	3.37E-65	-1.69E-46
MS.gene050105	MS.gene36571	0.824365	8.40E-54	-1.69E-46
MS.gene050414	MS.gene36571	0.822192	2.70E-53	-1.69E-46
MS.gene050571	MS.gene36571	0.80121	1.00E-48	-1.69E-46
MS.gene050646	MS.gene36571	0.820605	6.28E-53	-1.69E-46
MS.gene050654	MS.gene36571	0.835188	1.94E-56	-1.69E-46
MS.gene051122	MS.gene36571	0.827966	1.17E-54	-1.69E-46
MS.gene051209	MS.gene36571	0.806226	9.12E-50	-1.69E-46
MS.gene051275	MS.gene36571	0.851416	9.00E-61	-1.69E-46
MS.gene051332	MS.gene36571	0.819981	8.73E-53	-1.69E-46
MS.gene051368	MS.gene36571	0.805167	1.52E-49	-1.69E-46
MS.gene051637	MS.gene36571	0.812564	3.98E-51	-1.69E-46
MS.gene051856	MS.gene36571	0.814615	1.41E-51	-1.69E-46
MS.gene052016	MS.gene36571	0.825941	3.56E-54	-1.69E-46
MS.gene052118	MS.gene36571	0.840726	7.31E-58	-1.69E-46
MS.gene05216	MS.gene36571	0.824554	7.58E-54	-1.69E-46
MS.gene052463	MS.gene36571	0.817393	3.38E-52	-1.69E-46
MS.gene052534	MS.gene36571	0.821427	4.06E-53	-1.69E-46
MS.gene052674	MS.gene36571	0.829321	5.50E-55	-1.69E-46
MS.gene052675	MS.gene36571	0.826816	2.20E-54	-1.69E-46
MS.gene052677	MS.gene36571	0.827097	1.89E-54	-1.69E-46
MS.gene052954	MS.gene36571	0.816968	4.21E-52	-1.69E-46
MS.gene05308	MS.gene36571	0.80649	8.02E-50	-1.69E-46
MS.gene053272	MS.gene36571	-0.815196	1.05E-51	-1.69E-46
MS.gene053298	MS.gene36571	0.820105	8.18E-53	-1.69E-46
MS.gene053726	MS.gene36571	0.813196	2.89E-51	-1.69E-46
MS.gene053879	MS.gene36571	0.801365	9.32E-49	-1.69E-46
MS.gene053931	MS.gene36571	0.844618	6.76E-59	-1.69E-46
MS.gene053963	MS.gene36571	0.825909	3.63E-54	-1.69E-46
MS.gene05423	MS.gene36571	0.802829	4.66E-49	-1.69E-46
MS.gene05439	MS.gene36571	0.803693	3.09E-49	-1.69E-46
MS.gene054479	MS.gene36571	0.840677	7.53E-58	-1.69E-46
MS.gene05458	MS.gene36571	0.801768	7.70E-49	-1.69E-46
MS.gene054651	MS.gene36571	0.813173	2.93E-51	-1.69E-46
MS.gene054892	MS.gene36571	0.882034	1.46E-70	-1.69E-46
MS.gene054893	MS.gene36571	0.885669	6.66E-72	-1.69E-46
MS.gene054957	MS.gene36571	0.830933	2.22E-55	-1.69E-46
MS.gene055150	MS.gene36571	0.835333	1.78E-56	-1.69E-46
MS.gene055222	MS.gene36571	0.800711	1.27E-48	-1.69E-46
MS.gene055724	MS.gene36571	0.807574	4.73E-50	-1.69E-46
MS.gene055841	MS.gene36571	0.845682	3.49E-59	-1.69E-46
MS.gene056045	MS.gene36571	0.821553	3.80E-53	-1.69E-46
MS.gene056064	MS.gene36571	0.800954	1.13E-48	-1.69E-46
MS.gene056082	MS.gene36571	0.809509	1.83E-50	-1.69E-46
MS.gene05615	MS.gene36571	0.801358	9.35E-49	-1.69E-46
MS.gene05624	MS.gene36571	0.834934	2.25E-56	-1.69E-46
MS.gene056363	MS.gene36571	0.806624	7.51E-50	-1.69E-46
MS.gene056699	MS.gene36571	0.820212	7.73E-53	-1.69E-46
MS.gene056776	MS.gene36571	0.805746	1.15E-49	-1.69E-46
MS.gene056779	MS.gene36571	0.805702	1.18E-49	-1.69E-46
MS.gene056834	MS.gene36571	0.836948	6.94E-57	-1.69E-46
MS.gene05686	MS.gene36571	0.833289	5.81E-56	-1.69E-46
MS.gene056973	MS.gene36571	0.839765	1.30E-57	-1.69E-46
MS.gene057071	MS.gene36571	0.813626	2.33E-51	-1.69E-46
MS.gene057102	MS.gene36571	0.813591	2.37E-51	-1.69E-46
MS.gene057155	MS.gene36571	0.859056	5.39E-63	-1.69E-46
MS.gene057174	MS.gene36571	0.819047	1.43E-52	-1.69E-46
MS.gene057208	MS.gene36571	0.882095	1.39E-70	-1.69E-46
MS.gene057371	MS.gene36571	0.853222	2.76E-61	-1.69E-46
MS.gene057611	MS.gene36571	0.806506	7.96E-50	-1.69E-46
MS.gene057622	MS.gene36571	0.831002	2.14E-55	-1.69E-46
MS.gene058008	MS.gene36571	0.807218	5.63E-50	-1.69E-46
MS.gene058081	MS.gene36571	0.824024	1.01E-53	-1.69E-46
MS.gene058095	MS.gene36571	0.818531	1.87E-52	-1.69E-46
MS.gene058159	MS.gene36571	0.830464	2.89E-55	-1.69E-46
MS.gene058223	MS.gene36571	-0.813877	2.05E-51	-1.69E-46
MS.gene058279	MS.gene36571	0.837482	5.07E-57	-1.69E-46
MS.gene058429	MS.gene36571	0.835293	1.83E-56	-1.69E-46
MS.gene058520	MS.gene36571	0.806858	6.71E-50	-1.69E-46
MS.gene058592	MS.gene36571	0.821645	3.62E-53	-1.69E-46
MS.gene058769	MS.gene36571	0.834104	3.63E-56	-1.69E-46
MS.gene058781	MS.gene36571	0.827628	1.41E-54	-1.69E-46
MS.gene058783	MS.gene36571	0.810946	8.95E-51	-1.69E-46
MS.gene05878	MS.gene36571	0.815011	1.15E-51	-1.69E-46
MS.gene059338	MS.gene36571	-0.800866	1.18E-48	-1.69E-46
MS.gene059439	MS.gene36571	0.804243	2.37E-49	-1.69E-46
MS.gene05952	MS.gene36571	0.864126	1.52E-64	-1.69E-46
MS.gene05956	MS.gene36571	0.809122	2.21E-50	-1.69E-46
MS.gene05985	MS.gene36571	-0.854176	1.46E-61	-1.69E-46
MS.gene060244	MS.gene36571	0.835349	1.77E-56	-1.69E-46
MS.gene060259	MS.gene36571	0.830878	2.29E-55	-1.69E-46
MS.gene060365	MS.gene36571	0.806535	7.85E-50	-1.69E-46
MS.gene060412	MS.gene36571	0.826838	2.18E-54	-1.69E-46
MS.gene060413	MS.gene36571	0.809191	2.14E-50	-1.69E-46
MS.gene060435	MS.gene36571	0.810048	1.40E-50	-1.69E-46
MS.gene060518	MS.gene36571	0.805612	1.23E-49	-1.69E-46
MS.gene060520	MS.gene36571	0.803033	4.23E-49	-1.69E-46
MS.gene060522	MS.gene36571	0.822492	2.30E-53	-1.69E-46
MS.gene060527	MS.gene36571	0.843163	1.66E-58	-1.69E-46
MS.gene060777	MS.gene36571	0.832449	9.40E-56	-1.69E-46
MS.gene060840	MS.gene36571	0.811861	5.67E-51	-1.69E-46
MS.gene060850	MS.gene36571	0.849175	3.83E-60	-1.69E-46
MS.gene060851	MS.gene36571	0.855503	6.04E-62	-1.69E-46
MS.gene060852	MS.gene36571	0.854217	1.43E-61	-1.69E-46
MS.gene060932	MS.gene36571	0.830364	3.06E-55	-1.69E-46
MS.gene060933	MS.gene36571	0.84061	7.84E-58	-1.69E-46
MS.gene060935	MS.gene36571	0.845494	3.92E-59	-1.69E-46
MS.gene060937	MS.gene36571	0.856718	2.66E-62	-1.69E-46
MS.gene061188	MS.gene36571	0.817306	3.53E-52	-1.69E-46
MS.gene06121	MS.gene36571	0.808334	3.26E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36571.t1	MTR_8g015560	96.203	79	3	0	1	79	1	79	2.49e-49	150

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 11 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAGTTGAGGTGGTGTCTA+TGG	0.235479	8.4:+75887071	MS.gene36571:CDS
CGTCAAGTTAGTGCTCGTTT+TGG	0.356065	8.4:+75887180	MS.gene36571:CDS
AGGAAAGAAGCTGAAAGAAA+TGG	0.356123	8.4:+75887098	MS.gene36571:CDS
CTCCTTCTTTAATTTGTGAA+TGG	0.374793	8.4:-75887010	None:intergenic
TGAAAGGTGAGATCAAGAAA+TGG	0.464102	8.4:+75887133	MS.gene36571:CDS
GAAAGGTGAGATCAAGAAAT+GGG	0.482363	8.4:+75887134	MS.gene36571:CDS
TACCATTCACAAATTAAAGA+AGG	0.518100	8.4:+75887008	MS.gene36571:CDS
ATGGATTCAAATAAGTTGAA+AGG	0.552282	8.4:+75887117	MS.gene36571:CDS
GAGGTGGTGTCTATGGAGGA+AGG	0.559344	8.4:+75887078	MS.gene36571:CDS
TGAGATCAAGAAATGGGCTA+AGG	0.607413	8.4:+75887140	MS.gene36571:CDS
AGTTGAGGTGGTGTCTATGG+AGG	0.609297	8.4:+75887074	MS.gene36571:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGAAATCTCTATCTTTTAA+TGG	+	chr8.4:75886979-75886998	MS.gene36571:CDS	20.0%
!	TACCATTCACAAATTAAAGA+AGG	+	chr8.4:75887008-75887027	MS.gene36571:CDS	25.0%
!!	AAATCTCTATCTTTTAATGG+TGG	+	chr8.4:75886982-75887001	MS.gene36571:CDS	25.0%
!!	ATGGATTCAAATAAGTTGAA+AGG	+	chr8.4:75887117-75887136	MS.gene36571:CDS	25.0%
!!	CTCCTTCTTTAATTTGTGAA+TGG	-	chr8.4:75887013-75887032	None:intergenic	30.0%
	AGGAAAGAAGCTGAAAGAAA+TGG	+	chr8.4:75887098-75887117	MS.gene36571:CDS	35.0%
	GAAAGGTGAGATCAAGAAAT+GGG	+	chr8.4:75887134-75887153	MS.gene36571:CDS	35.0%
	TGAAAGGTGAGATCAAGAAA+TGG	+	chr8.4:75887133-75887152	MS.gene36571:CDS	35.0%
!!	TTGTTTTTCTTCAGTTGAGG+TGG	+	chr8.4:75887062-75887081	MS.gene36571:CDS	35.0%
!!!	TCGTTGTTTTTCTTCAGTTG+AGG	+	chr8.4:75887059-75887078	MS.gene36571:CDS	35.0%
!!	TGAGATCAAGAAATGGGCTA+AGG	+	chr8.4:75887140-75887159	MS.gene36571:CDS	40.0%
	TTCAGTTGAGGTGGTGTCTA+TGG	+	chr8.4:75887071-75887090	MS.gene36571:CDS	45.0%
!	CGTCAAGTTAGTGCTCGTTT+TGG	+	chr8.4:75887180-75887199	MS.gene36571:CDS	45.0%
	AGTTGAGGTGGTGTCTATGG+AGG	+	chr8.4:75887074-75887093	MS.gene36571:CDS	50.0%
	GAGGTGGTGTCTATGGAGGA+AGG	+	chr8.4:75887078-75887097	MS.gene36571:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	75886970	75887209	75886970	ID=MS.gene36571
chr8.4	mRNA	75886970	75887209	75886970	ID=MS.gene36571.t1;Parent=MS.gene36571
chr8.4	exon	75886970	75887209	75886970	ID=MS.gene36571.t1.exon1;Parent=MS.gene36571.t1
chr8.4	CDS	75886970	75887209	75886970	ID=cds.MS.gene36571.t1;Parent=MS.gene36571.t1

Gene Sequence	>MS.gene36571 ATGTCTGTGAAGAAATCTCTATCTTTTAATGGTGGAAGTACCATTCACAAATTAAAGAAGGAGAAATCTACAAAACGAAGACGTTTTGATCGTTGTTTTTCTTCAGTTGAGGTGGTGTCTATGGAGGAAGGAAAGAAGCTGAAAGAAATGGATTCAAATAAGTTGAAAGGTGAGATCAAGAAATGGGCTAAGGCTGTTGTTGCATATGCTCGTCAAGTTAGTGCTCGTTTTGGAAGCTAG
Protein sequence	>MS.gene36571.t1 MSVKKSLSFNGGSTIHKLKKEKSTKRRRFDRCFSSVEVVSMEEGKKLKEMDSNKLKGEIKKWAKAVVAYARQVSARFGS