Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064243.t1 | XP_013467585.1 | 99.1 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 1.40E-55 | 225.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064243.t1 | Q9LSQ6 | 67.6 | 105 | 33 | 1 | 6 | 109 | 19 | 123 | 2.3e-33 | 142.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064243.t1 | I3SZY1 | 99.1 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 1.0e-55 | 225.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene064243 | 0.823703 | 1.20E-53 | -1.69E-46 |
| MS.gene04918 | MS.gene064243 | 0.807509 | 4.88E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene064243 | 0.806213 | 9.17E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene064243 | 0.815526 | 8.84E-52 | -1.69E-46 |
| MS.gene049350 | MS.gene064243 | 0.806573 | 7.71E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene064243 | 0.820502 | 6.63E-53 | -1.69E-46 |
| MS.gene049711 | MS.gene064243 | 0.828641 | 8.03E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene064243 | 0.851269 | 9.90E-61 | -1.69E-46 |
| MS.gene049983 | MS.gene064243 | 0.831136 | 1.98E-55 | -1.69E-46 |
| MS.gene04998 | MS.gene064243 | 0.835868 | 1.31E-56 | -1.69E-46 |
| MS.gene050224 | MS.gene064243 | 0.83585 | 1.32E-56 | -1.69E-46 |
| MS.gene050253 | MS.gene064243 | 0.800237 | 1.58E-48 | -1.69E-46 |
| MS.gene050273 | MS.gene064243 | 0.802425 | 5.65E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene064243 | 0.849623 | 2.87E-60 | -1.69E-46 |
| MS.gene050414 | MS.gene064243 | 0.872613 | 2.78E-67 | -1.69E-46 |
| MS.gene050551 | MS.gene064243 | 0.803695 | 3.08E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene064243 | 0.802073 | 6.67E-49 | -1.69E-46 |
| MS.gene050561 | MS.gene064243 | 0.800321 | 1.52E-48 | -1.69E-46 |
| MS.gene050562 | MS.gene064243 | 0.805805 | 1.12E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene064243 | 0.827161 | 1.82E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene064243 | 0.827911 | 1.20E-54 | -1.69E-46 |
| MS.gene050759 | MS.gene064243 | 0.809609 | 1.74E-50 | -1.69E-46 |
| MS.gene050962 | MS.gene064243 | 0.801596 | 8.36E-49 | -1.69E-46 |
| MS.gene051029 | MS.gene064243 | 0.807217 | 5.63E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene064243 | 0.802548 | 5.32E-49 | -1.69E-46 |
| MS.gene051201 | MS.gene064243 | 0.81133 | 7.39E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene064243 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene051234 | MS.gene064243 | 0.82018 | 7.86E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene064243 | 0.846834 | 1.69E-59 | -1.69E-46 |
| MS.gene051343 | MS.gene064243 | 0.809721 | 1.65E-50 | -1.69E-46 |
| MS.gene051490 | MS.gene064243 | 0.815273 | 1.01E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene064243 | 0.814766 | 1.30E-51 | -1.69E-46 |
| MS.gene051819 | MS.gene064243 | 0.807569 | 4.74E-50 | -1.69E-46 |
| MS.gene051852 | MS.gene064243 | 0.853364 | 2.51E-61 | -1.69E-46 |
| MS.gene052016 | MS.gene064243 | 0.880561 | 4.96E-70 | -1.69E-46 |
| MS.gene052037 | MS.gene064243 | 0.812601 | 3.91E-51 | -1.69E-46 |
| MS.gene052242 | MS.gene064243 | -0.800927 | 1.15E-48 | -1.69E-46 |
| MS.gene05224 | MS.gene064243 | 0.815683 | 8.15E-52 | -1.69E-46 |
| MS.gene052307 | MS.gene064243 | 0.862107 | 6.40E-64 | -1.69E-46 |
| MS.gene052335 | MS.gene064243 | 0.843602 | 1.27E-58 | -1.69E-46 |
| MS.gene052459 | MS.gene064243 | 0.801829 | 7.49E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene064243 | 0.870837 | 1.08E-66 | -1.69E-46 |
| MS.gene052493 | MS.gene064243 | 0.819907 | 9.07E-53 | -1.69E-46 |
| MS.gene052514 | MS.gene064243 | 0.832952 | 7.05E-56 | -1.69E-46 |
| MS.gene052516 | MS.gene064243 | 0.825764 | 3.92E-54 | -1.69E-46 |
| MS.gene052533 | MS.gene064243 | 0.850523 | 1.61E-60 | -1.69E-46 |
| MS.gene052609 | MS.gene064243 | 0.862068 | 6.58E-64 | -1.69E-46 |
| MS.gene052932 | MS.gene064243 | 0.805895 | 1.07E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene064243 | 0.844615 | 6.77E-59 | -1.69E-46 |
| MS.gene053143 | MS.gene064243 | 0.861489 | 9.90E-64 | -1.69E-46 |
| MS.gene053144 | MS.gene064243 | 0.81989 | 9.16E-53 | -1.69E-46 |
| MS.gene053171 | MS.gene064243 | 0.841998 | 3.38E-58 | -1.69E-46 |
| MS.gene053581 | MS.gene064243 | 0.81958 | 1.08E-52 | -1.69E-46 |
| MS.gene053620 | MS.gene064243 | 0.831409 | 1.70E-55 | -1.69E-46 |
| MS.gene054178 | MS.gene064243 | 0.866576 | 2.58E-65 | -1.69E-46 |
| MS.gene05423 | MS.gene064243 | 0.806924 | 6.49E-50 | -1.69E-46 |
| MS.gene054554 | MS.gene064243 | 0.831508 | 1.60E-55 | -1.69E-46 |
| MS.gene054584 | MS.gene064243 | 0.822249 | 2.62E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene064243 | 0.808373 | 3.20E-50 | -1.69E-46 |
| MS.gene054617 | MS.gene064243 | 0.858064 | 1.06E-62 | -1.69E-46 |
| MS.gene054651 | MS.gene064243 | 0.806712 | 7.20E-50 | -1.69E-46 |
| MS.gene054772 | MS.gene064243 | 0.800177 | 1.63E-48 | -1.69E-46 |
| MS.gene054824 | MS.gene064243 | 0.833823 | 4.27E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene064243 | 0.859186 | 4.92E-63 | -1.69E-46 |
| MS.gene054893 | MS.gene064243 | 0.859069 | 5.34E-63 | -1.69E-46 |
| MS.gene055141 | MS.gene064243 | 0.804103 | 2.54E-49 | -1.69E-46 |
| MS.gene055229 | MS.gene064243 | 0.810605 | 1.06E-50 | -1.69E-46 |
| MS.gene055231 | MS.gene064243 | 0.800359 | 1.50E-48 | -1.69E-46 |
| MS.gene055386 | MS.gene064243 | 0.818694 | 1.72E-52 | -1.69E-46 |
| MS.gene05539 | MS.gene064243 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene064243 | 0.814112 | 1.82E-51 | -1.69E-46 |
| MS.gene055469 | MS.gene064243 | 0.834271 | 3.30E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene064243 | 0.866409 | 2.91E-65 | -1.69E-46 |
| MS.gene05552 | MS.gene064243 | 0.819038 | 1.43E-52 | -1.69E-46 |
| MS.gene055864 | MS.gene064243 | 0.835452 | 1.66E-56 | -1.69E-46 |
| MS.gene056082 | MS.gene064243 | 0.803564 | 3.28E-49 | -1.69E-46 |
| MS.gene056181 | MS.gene064243 | 0.804997 | 1.65E-49 | -1.69E-46 |
| MS.gene056285 | MS.gene064243 | 0.802155 | 6.41E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene064243 | 0.82022 | 7.69E-53 | -1.69E-46 |
| MS.gene056390 | MS.gene064243 | 0.821368 | 4.19E-53 | -1.69E-46 |
| MS.gene056507 | MS.gene064243 | 0.837419 | 5.26E-57 | -1.69E-46 |
| MS.gene056677 | MS.gene064243 | 0.838662 | 2.52E-57 | -1.69E-46 |
| MS.gene056699 | MS.gene064243 | 0.836478 | 9.14E-57 | -1.69E-46 |
| MS.gene05686 | MS.gene064243 | 0.828622 | 8.11E-55 | -1.69E-46 |
| MS.gene057034 | MS.gene064243 | 0.830754 | 2.46E-55 | -1.69E-46 |
| MS.gene057102 | MS.gene064243 | 0.83832 | 3.09E-57 | -1.69E-46 |
| MS.gene057294 | MS.gene064243 | 0.806091 | 9.73E-50 | -1.69E-46 |
| MS.gene057371 | MS.gene064243 | 0.808128 | 3.61E-50 | -1.69E-46 |
| MS.gene057919 | MS.gene064243 | 0.819753 | 9.84E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene064243 | 0.819304 | 1.25E-52 | -1.69E-46 |
| MS.gene058289 | MS.gene064243 | 0.822751 | 2.00E-53 | -1.69E-46 |
| MS.gene058330 | MS.gene064243 | 0.828079 | 1.10E-54 | -1.69E-46 |
| MS.gene058429 | MS.gene064243 | 0.807184 | 5.72E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene064243 | 0.818388 | 2.01E-52 | -1.69E-46 |
| MS.gene058649 | MS.gene064243 | 0.844995 | 5.35E-59 | -1.69E-46 |
| MS.gene058668 | MS.gene064243 | 0.826249 | 3.01E-54 | -1.69E-46 |
| MS.gene058720 | MS.gene064243 | 0.831642 | 1.49E-55 | -1.69E-46 |
| MS.gene059170 | MS.gene064243 | 0.849645 | 2.83E-60 | -1.69E-46 |
| MS.gene05952 | MS.gene064243 | 0.813681 | 2.26E-51 | -1.69E-46 |
| MS.gene059634 | MS.gene064243 | 0.818612 | 1.79E-52 | -1.69E-46 |
| MS.gene059774 | MS.gene064243 | 0.83377 | 4.41E-56 | -1.69E-46 |
| MS.gene05985 | MS.gene064243 | -0.813135 | 2.98E-51 | -1.69E-46 |
| MS.gene059965 | MS.gene064243 | 0.860432 | 2.07E-63 | -1.69E-46 |
| MS.gene060259 | MS.gene064243 | 0.830133 | 3.49E-55 | -1.69E-46 |
| MS.gene06052 | MS.gene064243 | 0.801944 | 7.09E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene064243 | 0.833512 | 5.11E-56 | -1.69E-46 |
| MS.gene060777 | MS.gene064243 | 0.803902 | 2.79E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene064243 | 0.857674 | 1.39E-62 | -1.69E-46 |
| MS.gene060933 | MS.gene064243 | 0.811817 | 5.79E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene064243 | 0.813689 | 2.25E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene064243 | 0.86393 | 1.75E-64 | -1.69E-46 |
| MS.gene061160 | MS.gene064243 | 0.802607 | 5.18E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene064243 | 0.81392 | 2.00E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067296 | MS.gene064243 | PPI |
| MS.gene064243 | MS.gene84736 | PPI |
| MS.gene064243 | MS.gene060524 | PPI |
| MS.gene064243 | MS.gene068384 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064243.t1 | MTR_1g052885 | 99.099 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 3.88e-75 | 218 |
| MS.gene064243.t1 | MTR_2g089210 | 86.667 | 105 | 14 | 0 | 6 | 110 | 10 | 114 | 1.30e-60 | 181 |
| MS.gene064243.t1 | MTR_2g081580 | 69.444 | 108 | 33 | 0 | 6 | 113 | 10 | 117 | 4.40e-48 | 150 |
| MS.gene064243.t1 | MTR_8g466250 | 66.667 | 105 | 35 | 0 | 6 | 110 | 11 | 115 | 1.08e-44 | 141 |
| MS.gene064243.t1 | MTR_2g011400 | 60.396 | 101 | 40 | 0 | 6 | 106 | 14 | 114 | 2.09e-38 | 125 |
| MS.gene064243.t1 | MTR_8g027180 | 52.381 | 105 | 50 | 0 | 6 | 110 | 19 | 123 | 6.80e-35 | 117 |
| MS.gene064243.t1 | MTR_2g081300 | 62.637 | 91 | 33 | 1 | 6 | 96 | 5 | 94 | 7.23e-33 | 111 |
| MS.gene064243.t1 | MTR_2g081350 | 61.538 | 91 | 34 | 1 | 6 | 96 | 9 | 98 | 1.62e-31 | 108 |
| MS.gene064243.t1 | MTR_2g081440 | 61.538 | 91 | 34 | 1 | 6 | 96 | 9 | 98 | 2.96e-31 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064243.t1 | AT5G54490 | 66.981 | 106 | 34 | 1 | 6 | 110 | 19 | 124 | 1.93e-45 | 143 |
| MS.gene064243.t1 | AT4G27280 | 66.355 | 107 | 35 | 1 | 6 | 111 | 19 | 125 | 2.37e-43 | 138 |
| MS.gene064243.t1 | AT2G46600 | 54.054 | 111 | 51 | 0 | 6 | 116 | 22 | 132 | 1.01e-38 | 127 |
Find 22 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGACTCTTCCATCAATTC+AGG | 0.351816 | 4.2:+54901570 | None:intergenic |
| TGATGGAAGAGTCTTGGTTT+TGG | 0.354264 | 4.2:-54901563 | MS.gene064243:CDS |
| AGGTGGTGAAGGTTTAATAA+AGG | 0.428490 | 4.2:-54901793 | MS.gene064243:CDS |
| CTGAATTGATGGAAGAGTCT+TGG | 0.441368 | 4.2:-54901569 | MS.gene064243:CDS |
| TTGAAGGAAGATGAACTTGT+TGG | 0.457469 | 4.2:-54901672 | MS.gene064243:CDS |
| TTGTTGATGGACAAAGAAAA+AGG | 0.459319 | 4.2:-54901750 | MS.gene064243:CDS |
| CCTAGCTTGTTTGCCATCAC+TGG | 0.464938 | 4.2:+54901813 | None:intergenic |
| TGAAGACTTGTTACCAGTGA+TGG | 0.470070 | 4.2:-54901826 | MS.gene064243:CDS |
| TTTGGGTTTACAAGATTTGA+AGG | 0.473694 | 4.2:-54901688 | MS.gene064243:CDS |
| TGATAACATTGGATAGTTTG+AGG | 0.497485 | 4.2:-54901725 | MS.gene064243:CDS |
| GAAGGTGATTTAGATAGAGA+TGG | 0.504919 | 4.2:-54901639 | MS.gene064243:CDS |
| TAGGTTGAGTCCTGAATTGA+TGG | 0.511296 | 4.2:-54901580 | MS.gene064243:CDS |
| TAACAAGTCTTCAAAACCAT+TGG | 0.541959 | 4.2:+54901836 | None:intergenic |
| GAAGCTCTTCAACATGAACT+TGG | 0.544590 | 4.2:-54901534 | MS.gene064243:CDS |
| TTAATAAAGGAGCTATGCAA+TGG | 0.554583 | 4.2:-54901780 | MS.gene064243:CDS |
| GCAAACAAGCTAGGTGGTGA+AGG | 0.561539 | 4.2:-54901804 | MS.gene064243:CDS |
| CCAGTGATGGCAAACAAGCT+AGG | 0.566082 | 4.2:-54901813 | MS.gene064243:CDS |
| AGATGGAGCACTTACTCAGA+TGG | 0.568398 | 4.2:-54901622 | MS.gene064243:CDS |
| TTATCAACAATGGCTACCAA+TGG | 0.580721 | 4.2:-54901852 | None:intergenic |
| CTTGTTGGTATGATGAATGA+AGG | 0.583851 | 4.2:-54901657 | MS.gene064243:CDS |
| ATTGTTCCTAGGAAACTACA+AGG | 0.600196 | 4.2:+54901497 | None:intergenic |
| GTGATGGCAAACAAGCTAGG+TGG | 0.738373 | 4.2:-54901810 | MS.gene064243:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTGTGTTTTGATGTTT+AGG | - | chr4.2:54901756-54901775 | MS.gene064243:CDS | 25.0% |
| TAACAAGTCTTCAAAACCAT+TGG | + | chr4.2:54901522-54901541 | None:intergenic | 30.0% | |
| TGATAACATTGGATAGTTTG+AGG | - | chr4.2:54901630-54901649 | MS.gene064243:CDS | 30.0% | |
| TTAATAAAGGAGCTATGCAA+TGG | - | chr4.2:54901575-54901594 | MS.gene064243:CDS | 30.0% | |
| ! | AGAAAAAGGTGTGATAACAT+TGG | - | chr4.2:54901619-54901638 | MS.gene064243:CDS | 30.0% |
| ! | TTGTTGATGGACAAAGAAAA+AGG | - | chr4.2:54901605-54901624 | MS.gene064243:CDS | 30.0% |
| ! | TTTGGGTTTACAAGATTTGA+AGG | - | chr4.2:54901667-54901686 | MS.gene064243:CDS | 30.0% |
| !!! | CAATGGTTTTGAATTGTTGA+TGG | - | chr4.2:54901592-54901611 | MS.gene064243:CDS | 30.0% |
| AGGTGGTGAAGGTTTAATAA+AGG | - | chr4.2:54901562-54901581 | MS.gene064243:CDS | 35.0% | |
| CTTGTTGGTATGATGAATGA+AGG | - | chr4.2:54901698-54901717 | MS.gene064243:CDS | 35.0% | |
| GAAGGTGATTTAGATAGAGA+TGG | - | chr4.2:54901716-54901735 | MS.gene064243:CDS | 35.0% | |
| TTGAAGGAAGATGAACTTGT+TGG | - | chr4.2:54901683-54901702 | MS.gene064243:CDS | 35.0% | |
| CAAGACTCTTCCATCAATTC+AGG | + | chr4.2:54901788-54901807 | None:intergenic | 40.0% | |
| CTGAATTGATGGAAGAGTCT+TGG | - | chr4.2:54901786-54901805 | MS.gene064243:CDS | 40.0% | |
| GAAGCTCTTCAACATGAACT+TGG | - | chr4.2:54901821-54901840 | MS.gene064243:CDS | 40.0% | |
| ! | TAGGTTGAGTCCTGAATTGA+TGG | - | chr4.2:54901775-54901794 | MS.gene064243:CDS | 40.0% |
| ! | TGATGGAAGAGTCTTGGTTT+TGG | - | chr4.2:54901792-54901811 | MS.gene064243:CDS | 40.0% |
| !! | TGAAGACTTGTTACCAGTGA+TGG | - | chr4.2:54901529-54901548 | MS.gene064243:CDS | 40.0% |
| !!! | AGGAAAAACGCTGCTGTTTT+GGG | - | chr4.2:54901650-54901669 | MS.gene064243:CDS | 40.0% |
| ! | AGATGGAGCACTTACTCAGA+TGG | - | chr4.2:54901733-54901752 | MS.gene064243:CDS | 45.0% |
| !! | GAGGAAAAACGCTGCTGTTT+TGG | - | chr4.2:54901649-54901668 | MS.gene064243:CDS | 45.0% |
| CCAGTGATGGCAAACAAGCT+AGG | - | chr4.2:54901542-54901561 | MS.gene064243:CDS | 50.0% | |
| GCAAACAAGCTAGGTGGTGA+AGG | - | chr4.2:54901551-54901570 | MS.gene064243:CDS | 50.0% | |
| GTGATGGCAAACAAGCTAGG+TGG | - | chr4.2:54901545-54901564 | MS.gene064243:CDS | 50.0% | |
| !! | CCTAGCTTGTTTGCCATCAC+TGG | + | chr4.2:54901545-54901564 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 54901512 | 54901865 | 54901512 | ID=MS.gene064243 |
| chr4.2 | mRNA | 54901512 | 54901865 | 54901512 | ID=MS.gene064243.t1;Parent=MS.gene064243 |
| chr4.2 | exon | 54901512 | 54901865 | 54901512 | ID=MS.gene064243.t1.exon1;Parent=MS.gene064243.t1 |
| chr4.2 | CDS | 54901512 | 54901865 | 54901512 | ID=cds.MS.gene064243.t1;Parent=MS.gene064243.t1 |
| Gene Sequence |
| Protein sequence |
