Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90533.t1 | XP_003596532.1 | 100 | 102 | 0 | 0 | 1 | 102 | 17 | 118 | 2.30E-49 | 204.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90533.t1 | O81831 | 66.3 | 98 | 32 | 1 | 1 | 97 | 26 | 123 | 6.0e-30 | 131.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90533.t1 | B7FH70 | 100.0 | 102 | 0 | 0 | 1 | 102 | 17 | 118 | 1.7e-49 | 204.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene90533 | 0.85351 | 2.28E-61 | -1.69E-46 |
| MS.gene051226 | MS.gene90533 | 0.801657 | 8.12E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene90533 | 0.80601 | 1.01E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene90533 | 0.800091 | 1.70E-48 | -1.69E-46 |
| MS.gene061047 | MS.gene90533 | 0.800861 | 1.18E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90533 | MS.gene060524 | PPI |
| MS.gene90533 | MS.gene84736 | PPI |
| MS.gene90533 | MS.gene068384 | PPI |
| MS.gene90533 | MS.gene067296 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90533.t1 | MTR_2g081580 | 100.000 | 102 | 0 | 0 | 1 | 102 | 17 | 118 | 4.61e-69 | 202 |
| MS.gene90533.t1 | MTR_8g466250 | 81.443 | 97 | 18 | 0 | 1 | 97 | 18 | 114 | 4.69e-52 | 159 |
| MS.gene90533.t1 | MTR_1g052885 | 70.408 | 98 | 29 | 0 | 1 | 98 | 13 | 110 | 3.27e-43 | 137 |
| MS.gene90533.t1 | MTR_2g089210 | 68.687 | 99 | 31 | 0 | 1 | 99 | 17 | 115 | 3.74e-42 | 134 |
| MS.gene90533.t1 | MTR_2g011400 | 64.894 | 94 | 33 | 0 | 1 | 94 | 21 | 114 | 5.86e-37 | 121 |
| MS.gene90533.t1 | MTR_2g081300 | 67.045 | 88 | 28 | 1 | 1 | 88 | 12 | 98 | 5.41e-35 | 116 |
| MS.gene90533.t1 | MTR_2g081350 | 65.169 | 89 | 30 | 1 | 1 | 89 | 16 | 103 | 2.91e-34 | 114 |
| MS.gene90533.t1 | MTR_2g081440 | 62.500 | 88 | 32 | 1 | 1 | 88 | 16 | 102 | 1.69e-31 | 107 |
| MS.gene90533.t1 | MTR_8g027180 | 47.000 | 100 | 53 | 0 | 1 | 100 | 26 | 125 | 2.27e-31 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90533.t1 | AT5G54490 | 66.327 | 98 | 32 | 1 | 1 | 97 | 26 | 123 | 3.71e-41 | 132 |
| MS.gene90533.t1 | AT4G27280 | 66.327 | 98 | 32 | 1 | 1 | 97 | 26 | 123 | 4.78e-41 | 132 |
| MS.gene90533.t1 | AT2G46600 | 52.041 | 98 | 47 | 0 | 1 | 98 | 29 | 126 | 2.89e-33 | 112 |
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGACTTAGTCCTGAACTTA+TGG | 0.262199 | 2.3:-16664533 | MS.gene90533:CDS |
| AAAATCACCTTCAACAATCA+TGG | 0.442153 | 2.3:+16664603 | None:intergenic |
| AAAAGAATTCAGCTTTGCTT+GGG | 0.443368 | 2.3:-16664657 | MS.gene90533:CDS |
| CTTCAATCCATGATTGTTGA+AGG | 0.479434 | 2.3:-16664610 | MS.gene90533:CDS |
| TTAGACCCATCCATAAGTTC+AGG | 0.481132 | 2.3:+16664523 | None:intergenic |
| GCAAACAAGCTTGGTGGGGA+TGG | 0.482564 | 2.3:-16664757 | MS.gene90533:CDS |
| CCCCTCATGGCAAACAAGCT+TGG | 0.492739 | 2.3:-16664766 | None:intergenic |
| CTCATGGCAAACAAGCTTGG+TGG | 0.508830 | 2.3:-16664763 | MS.gene90533:CDS |
| TCATGGCAAACAAGCTTGGT+GGG | 0.509220 | 2.3:-16664762 | MS.gene90533:CDS |
| CTTAGTCCTGAACTTATGGA+TGG | 0.521531 | 2.3:-16664529 | MS.gene90533:CDS |
| TGATGGTGCTCTTAATCAAA+TGG | 0.521789 | 2.3:-16664575 | MS.gene90533:CDS |
| TTAATAGATGAATTATGCAA+TGG | 0.547432 | 2.3:-16664733 | MS.gene90533:CDS |
| TTTGATGGATTCTACTAAAG+GGG | 0.572126 | 2.3:-16664701 | MS.gene90533:CDS |
| GGATGGGTCTAAGATGTGGT+TGG | 0.605170 | 2.3:-16664512 | MS.gene90533:CDS |
| GTTACAAGATTTGACCGATG+TGG | 0.613980 | 2.3:-16664635 | MS.gene90533:CDS |
| TTAGTCCTGAACTTATGGAT+GGG | 0.620182 | 2.3:-16664528 | MS.gene90533:CDS |
| CATGGCAAACAAGCTTGGTG+GGG | 0.632642 | 2.3:-16664761 | MS.gene90533:CDS |
| TTATGGATGGGTCTAAGATG+TGG | 0.687819 | 2.3:-16664516 | MS.gene90533:CDS |
| CATGGATTGAAGTTCCACAT+CGG | 0.713256 | 2.3:+16664621 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATAGATGAATTATGCAA+TGG | - | chr2.3:16664501-16664520 | MS.gene90533:CDS | 20.0% |
| !!! | ATTCTTTTTAAGGCTTTCAA+AGG | + | chr2.3:16664565-16664584 | None:intergenic | 25.0% |
| !!! | TTTTGATGGATTCTACTAAA+GGG | - | chr2.3:16664532-16664551 | MS.gene90533:CDS | 25.0% |
| AAAAAGAATTCAGCTTTGCT+TGG | - | chr2.3:16664576-16664595 | MS.gene90533:CDS | 30.0% | |
| AAAAGAATTCAGCTTTGCTT+GGG | - | chr2.3:16664577-16664596 | MS.gene90533:CDS | 30.0% | |
| AAAATCACCTTCAACAATCA+TGG | + | chr2.3:16664634-16664653 | None:intergenic | 30.0% | |
| ! | ATTGTTGAAGGTGATTTTGA+TGG | - | chr2.3:16664636-16664655 | MS.gene90533:CDS | 30.0% |
| ! | CAATGGTTTCAATCTTTTGA+TGG | - | chr2.3:16664518-16664537 | MS.gene90533:CDS | 30.0% |
| ! | GCAAAGCTGAATTCTTTTTA+AGG | + | chr2.3:16664575-16664594 | None:intergenic | 30.0% |
| !! | TTTGATGGATTCTACTAAAG+GGG | - | chr2.3:16664533-16664552 | MS.gene90533:CDS | 30.0% |
| !!! | CTTTTGATGGATTCTACTAA+AGG | - | chr2.3:16664531-16664550 | MS.gene90533:CDS | 30.0% |
| CTTCAATCCATGATTGTTGA+AGG | - | chr2.3:16664624-16664643 | MS.gene90533:CDS | 35.0% | |
| TAGACTTAGTCCTGAACTTA+TGG | - | chr2.3:16664701-16664720 | MS.gene90533:CDS | 35.0% | |
| TTAGTCCTGAACTTATGGAT+GGG | - | chr2.3:16664706-16664725 | MS.gene90533:CDS | 35.0% | |
| ! | TGATGGTGCTCTTAATCAAA+TGG | - | chr2.3:16664659-16664678 | MS.gene90533:CDS | 35.0% |
| CTTAGTCCTGAACTTATGGA+TGG | - | chr2.3:16664705-16664724 | MS.gene90533:CDS | 40.0% | |
| GTTACAAGATTTGACCGATG+TGG | - | chr2.3:16664599-16664618 | MS.gene90533:CDS | 40.0% | |
| TTAGACCCATCCATAAGTTC+AGG | + | chr2.3:16664714-16664733 | None:intergenic | 40.0% | |
| ! | CATGGATTGAAGTTCCACAT+CGG | + | chr2.3:16664616-16664635 | None:intergenic | 40.0% |
| ! | TTATGGATGGGTCTAAGATG+TGG | - | chr2.3:16664718-16664737 | MS.gene90533:CDS | 40.0% |
| !!! | GAAGGTGATTTTGATGGTGA+TGG | - | chr2.3:16664642-16664661 | MS.gene90533:CDS | 40.0% |
| ! | GGATGGGTCTAAGATGTGGT+TGG | - | chr2.3:16664722-16664741 | MS.gene90533:CDS | 50.0% |
| ! | GCAAACAAGCTTGGTGGGGA+TGG | - | chr2.3:16664477-16664496 | MS.gene90533:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 16664474 | 16664782 | 16664474 | ID=MS.gene90533 |
| chr2.3 | mRNA | 16664474 | 16664782 | 16664474 | ID=MS.gene90533.t1;Parent=MS.gene90533 |
| chr2.3 | exon | 16664474 | 16664782 | 16664474 | ID=MS.gene90533.t1.exon1;Parent=MS.gene90533.t1 |
| chr2.3 | CDS | 16664474 | 16664782 | 16664474 | ID=cds.MS.gene90533.t1;Parent=MS.gene90533.t1 |
| Gene Sequence |
| Protein sequence |
