AlfalfaGEDB | MS.gene51384

M. sativa cultivar XinJiangDaYe / MS.gene51384

KEGG: K24345
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13833.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51384.t1	XP_013467585.1	99.1	111	1	0	1	111	1	111	1.40E-55	225.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51384.t1	Q9LSQ6	67.6	105	33	1	6	109	19	123	2.3e-33	142.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51384.t1	I3SZY1	99.1	111	1	0	1	111	1	111	1.0e-55	225.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene51384	0.828162	1.05E-54	-1.69E-46
MS.gene049350	MS.gene51384	0.816404	5.63E-52	-1.69E-46
MS.gene049368	MS.gene51384	0.812835	3.47E-51	-1.69E-46
MS.gene049711	MS.gene51384	0.818475	1.92E-52	-1.69E-46
MS.gene049807	MS.gene51384	0.8479	8.63E-60	-1.69E-46
MS.gene049887	MS.gene51384	0.809091	2.25E-50	-1.69E-46
MS.gene049983	MS.gene51384	0.824607	7.37E-54	-1.69E-46
MS.gene04998	MS.gene51384	0.824296	8.72E-54	-1.69E-46
MS.gene050224	MS.gene51384	0.834629	2.68E-56	-1.69E-46
MS.gene050253	MS.gene51384	0.808812	2.58E-50	-1.69E-46
MS.gene050359	MS.gene51384	0.852476	4.50E-61	-1.69E-46
MS.gene050414	MS.gene51384	0.852092	5.79E-61	-1.69E-46
MS.gene050551	MS.gene51384	0.81623	6.16E-52	-1.69E-46
MS.gene050646	MS.gene51384	0.822045	2.92E-53	-1.69E-46
MS.gene050654	MS.gene51384	0.815049	1.13E-51	-1.69E-46
MS.gene050838	MS.gene51384	0.8082	3.48E-50	-1.69E-46
MS.gene051102	MS.gene51384	0.810093	1.37E-50	-1.69E-46
MS.gene051234	MS.gene51384	0.821636	3.63E-53	-1.69E-46
MS.gene051279	MS.gene51384	0.816339	5.82E-52	-1.69E-46
MS.gene051490	MS.gene51384	0.818107	2.33E-52	-1.69E-46
MS.gene051676	MS.gene51384	0.810235	1.28E-50	-1.69E-46
MS.gene051819	MS.gene51384	0.829792	4.22E-55	-1.69E-46
MS.gene051852	MS.gene51384	0.865877	4.29E-65	-1.69E-46
MS.gene052016	MS.gene51384	0.85702	2.17E-62	-1.69E-46
MS.gene05224	MS.gene51384	0.819143	1.36E-52	-1.69E-46
MS.gene052307	MS.gene51384	0.865955	4.06E-65	-1.69E-46
MS.gene052335	MS.gene51384	0.831002	2.14E-55	-1.69E-46
MS.gene052342	MS.gene51384	0.8094	1.93E-50	-1.69E-46
MS.gene052459	MS.gene51384	0.808593	2.87E-50	-1.69E-46
MS.gene052463	MS.gene51384	0.86038	2.15E-63	-1.69E-46
MS.gene052493	MS.gene51384	0.801757	7.74E-49	-1.69E-46
MS.gene052514	MS.gene51384	0.806184	9.31E-50	-1.69E-46
MS.gene052516	MS.gene51384	0.827054	1.93E-54	-1.69E-46
MS.gene052533	MS.gene51384	0.831671	1.46E-55	-1.69E-46
MS.gene052609	MS.gene51384	0.864302	1.34E-64	-1.69E-46
MS.gene052932	MS.gene51384	0.805085	1.58E-49	-1.69E-46
MS.gene053079	MS.gene51384	0.843618	1.25E-58	-1.69E-46
MS.gene053143	MS.gene51384	0.865214	6.94E-65	-1.69E-46
MS.gene053144	MS.gene51384	0.821645	3.62E-53	-1.69E-46
MS.gene053171	MS.gene51384	0.815409	9.38E-52	-1.69E-46
MS.gene053456	MS.gene51384	0.800604	1.33E-48	-1.69E-46
MS.gene053581	MS.gene51384	0.817072	3.99E-52	-1.69E-46
MS.gene053620	MS.gene51384	0.808543	2.94E-50	-1.69E-46
MS.gene054178	MS.gene51384	0.848907	4.54E-60	-1.69E-46
MS.gene054554	MS.gene51384	0.810231	1.28E-50	-1.69E-46
MS.gene054584	MS.gene51384	0.82346	1.37E-53	-1.69E-46
MS.gene05458	MS.gene51384	0.817978	2.49E-52	-1.69E-46
MS.gene054617	MS.gene51384	0.850004	2.25E-60	-1.69E-46
MS.gene054824	MS.gene51384	0.841425	4.79E-58	-1.69E-46
MS.gene054892	MS.gene51384	0.834945	2.23E-56	-1.69E-46
MS.gene054893	MS.gene51384	0.833942	3.99E-56	-1.69E-46
MS.gene055313	MS.gene51384	0.807169	5.76E-50	-1.69E-46
MS.gene055465	MS.gene51384	0.810936	9.00E-51	-1.69E-46
MS.gene055469	MS.gene51384	0.836821	7.47E-57	-1.69E-46
MS.gene055513	MS.gene51384	0.875605	2.70E-68	-1.69E-46
MS.gene05552	MS.gene51384	0.804031	2.63E-49	-1.69E-46
MS.gene05574	MS.gene51384	0.816013	6.88E-52	-1.69E-46
MS.gene055864	MS.gene51384	0.834875	2.33E-56	-1.69E-46
MS.gene056181	MS.gene51384	0.832837	7.53E-56	-1.69E-46
MS.gene056308	MS.gene51384	0.822766	1.99E-53	-1.69E-46
MS.gene056390	MS.gene51384	0.827815	1.27E-54	-1.69E-46
MS.gene056507	MS.gene51384	0.83958	1.46E-57	-1.69E-46
MS.gene056677	MS.gene51384	0.808349	3.24E-50	-1.69E-46
MS.gene056699	MS.gene51384	0.828924	6.86E-55	-1.69E-46
MS.gene057034	MS.gene51384	0.825749	3.96E-54	-1.69E-46
MS.gene057102	MS.gene51384	0.819492	1.13E-52	-1.69E-46
MS.gene057919	MS.gene51384	0.812547	4.01E-51	-1.69E-46
MS.gene058289	MS.gene51384	0.820782	5.72E-53	-1.69E-46
MS.gene058330	MS.gene51384	0.821342	4.25E-53	-1.69E-46
MS.gene058492	MS.gene51384	0.808446	3.08E-50	-1.69E-46
MS.gene058638	MS.gene51384	0.808529	2.96E-50	-1.69E-46
MS.gene058639	MS.gene51384	0.813756	2.18E-51	-1.69E-46
MS.gene058649	MS.gene51384	0.836612	8.45E-57	-1.69E-46
MS.gene058668	MS.gene51384	0.826253	3.00E-54	-1.69E-46
MS.gene058720	MS.gene51384	0.845222	4.64E-59	-1.69E-46
MS.gene058758	MS.gene51384	0.826697	2.35E-54	-1.69E-46
MS.gene059170	MS.gene51384	0.847792	9.24E-60	-1.69E-46
MS.gene059634	MS.gene51384	0.804944	1.69E-49	-1.69E-46
MS.gene059774	MS.gene51384	0.824147	9.45E-54	-1.69E-46
MS.gene059965	MS.gene51384	0.860042	2.72E-63	-1.69E-46
MS.gene060259	MS.gene51384	0.803518	3.36E-49	-1.69E-46
MS.gene060343	MS.gene51384	-0.807696	4.45E-50	-1.69E-46
MS.gene060637	MS.gene51384	0.803563	3.28E-49	-1.69E-46
MS.gene060697	MS.gene51384	0.825119	5.58E-54	-1.69E-46
MS.gene060932	MS.gene51384	0.848063	7.78E-60	-1.69E-46
MS.gene060933	MS.gene51384	0.803781	2.96E-49	-1.69E-46
MS.gene061006	MS.gene51384	0.826776	2.25E-54	-1.69E-46
MS.gene061100	MS.gene51384	0.845918	3.01E-59	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene067296	MS.gene51384	PPI
MS.gene84736	MS.gene51384	PPI
MS.gene068384	MS.gene51384	PPI
MS.gene060524	MS.gene51384	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51384.t1	MTR_1g052885	99.099	111	1	0	1	111	1	111	3.88e-75	218
MS.gene51384.t1	MTR_2g089210	86.667	105	14	0	6	110	10	114	1.30e-60	181
MS.gene51384.t1	MTR_2g081580	69.444	108	33	0	6	113	10	117	4.40e-48	150
MS.gene51384.t1	MTR_8g466250	66.667	105	35	0	6	110	11	115	1.08e-44	141
MS.gene51384.t1	MTR_2g011400	60.396	101	40	0	6	106	14	114	2.09e-38	125
MS.gene51384.t1	MTR_8g027180	52.381	105	50	0	6	110	19	123	6.80e-35	117
MS.gene51384.t1	MTR_2g081300	62.637	91	33	1	6	96	5	94	7.23e-33	111
MS.gene51384.t1	MTR_2g081350	61.538	91	34	1	6	96	9	98	1.62e-31	108
MS.gene51384.t1	MTR_2g081440	61.538	91	34	1	6	96	9	98	2.96e-31	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51384.t1	AT5G54490	66.981	106	34	1	6	110	19	124	1.93e-45	143
MS.gene51384.t1	AT4G27280	66.355	107	35	1	6	111	19	125	2.37e-43	138
MS.gene51384.t1	AT2G46600	54.054	111	51	0	6	116	22	132	1.01e-38	127

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGACTCTTCCATCAATTC+AGG	0.351816	4.1:+52033513	None:intergenic
TGATGGAAGAGTCTTGGTTT+TGG	0.354264	4.1:-52033506	MS.gene51384:CDS
AGGTGGTGAGGGTTTAATAA+AGG	0.439391	4.1:-52033736	MS.gene51384:CDS
CTGAATTGATGGAAGAGTCT+TGG	0.441368	4.1:-52033512	MS.gene51384:CDS
TTGAAGGAAGATGAACTTGT+TGG	0.457469	4.1:-52033615	MS.gene51384:CDS
TTGTTGATGGACAAAGAAAA+AGG	0.459319	4.1:-52033693	MS.gene51384:CDS
CCTAGCTTGTTTGCCATCAC+TGG	0.464938	4.1:+52033756	None:intergenic
TGAAGACTTGTTACCAGTGA+TGG	0.470070	4.1:-52033769	MS.gene51384:CDS
TTTGGGTTTACAAGATTTGA+AGG	0.473694	4.1:-52033631	MS.gene51384:CDS
TGATAACATTGGATAGTTTG+AGG	0.497485	4.1:-52033668	MS.gene51384:CDS
GAAGGTGATTTAGATAGAGA+TGG	0.504919	4.1:-52033582	MS.gene51384:CDS
TAGGTTGAGTCCTGAATTGA+TGG	0.511296	4.1:-52033523	MS.gene51384:CDS
TAACAAGTCTTCAAAACCAT+TGG	0.541959	4.1:+52033779	None:intergenic
GAAGCTCTTCAACATGAACT+TGG	0.544590	4.1:-52033477	MS.gene51384:CDS
TTAATAAAGGAGCTATGCAA+TGG	0.554583	4.1:-52033723	MS.gene51384:CDS
GGCAAACAAGCTAGGTGGTG+AGG	0.564491	4.1:-52033748	MS.gene51384:CDS
CCAGTGATGGCAAACAAGCT+AGG	0.566082	4.1:-52033756	MS.gene51384:CDS
AGATGGAGCACTTACTCAGA+TGG	0.568398	4.1:-52033565	MS.gene51384:CDS
GCAAACAAGCTAGGTGGTGA+GGG	0.579388	4.1:-52033747	MS.gene51384:CDS
TTATCAACAATGGCTACCAA+TGG	0.580721	4.1:-52033795	None:intergenic
CTTGTTGGTATGATGAATGA+AGG	0.583851	4.1:-52033600	MS.gene51384:CDS
ACTGTTCCTAGGAAACTACA+AGG	0.627269	4.1:+52033440	None:intergenic
GTGATGGCAAACAAGCTAGG+TGG	0.738373	4.1:-52033753	MS.gene51384:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTTTGTGTTTTGATGTTT+AGG	-	chr4.1:52033699-52033718	MS.gene51384:CDS	25.0%
	TAACAAGTCTTCAAAACCAT+TGG	+	chr4.1:52033465-52033484	None:intergenic	30.0%
	TGATAACATTGGATAGTTTG+AGG	-	chr4.1:52033573-52033592	MS.gene51384:CDS	30.0%
	TTAATAAAGGAGCTATGCAA+TGG	-	chr4.1:52033518-52033537	MS.gene51384:CDS	30.0%
!	AGAAAAAGGTGTGATAACAT+TGG	-	chr4.1:52033562-52033581	MS.gene51384:CDS	30.0%
!	TTGTTGATGGACAAAGAAAA+AGG	-	chr4.1:52033548-52033567	MS.gene51384:CDS	30.0%
!	TTTGGGTTTACAAGATTTGA+AGG	-	chr4.1:52033610-52033629	MS.gene51384:CDS	30.0%
!!!	CAATGGTTTTGAATTGTTGA+TGG	-	chr4.1:52033535-52033554	MS.gene51384:CDS	30.0%
	CTTGTTGGTATGATGAATGA+AGG	-	chr4.1:52033641-52033660	MS.gene51384:CDS	35.0%
	GAAGGTGATTTAGATAGAGA+TGG	-	chr4.1:52033659-52033678	MS.gene51384:CDS	35.0%
	TTGAAGGAAGATGAACTTGT+TGG	-	chr4.1:52033626-52033645	MS.gene51384:CDS	35.0%
	AGGTGGTGAGGGTTTAATAA+AGG	-	chr4.1:52033505-52033524	MS.gene51384:CDS	40.0%
	CAAGACTCTTCCATCAATTC+AGG	+	chr4.1:52033731-52033750	None:intergenic	40.0%
	CTGAATTGATGGAAGAGTCT+TGG	-	chr4.1:52033729-52033748	MS.gene51384:CDS	40.0%
	GAAGCTCTTCAACATGAACT+TGG	-	chr4.1:52033764-52033783	MS.gene51384:CDS	40.0%
!	TAGGTTGAGTCCTGAATTGA+TGG	-	chr4.1:52033718-52033737	MS.gene51384:CDS	40.0%
!	TGATGGAAGAGTCTTGGTTT+TGG	-	chr4.1:52033735-52033754	MS.gene51384:CDS	40.0%
!!	TGAAGACTTGTTACCAGTGA+TGG	-	chr4.1:52033472-52033491	MS.gene51384:CDS	40.0%
!!!	AGGAAAAACGCTGCTGTTTT+GGG	-	chr4.1:52033593-52033612	MS.gene51384:CDS	40.0%
!	AGATGGAGCACTTACTCAGA+TGG	-	chr4.1:52033676-52033695	MS.gene51384:CDS	45.0%
!!	GAGGAAAAACGCTGCTGTTT+TGG	-	chr4.1:52033592-52033611	MS.gene51384:CDS	45.0%
	CCAGTGATGGCAAACAAGCT+AGG	-	chr4.1:52033485-52033504	MS.gene51384:CDS	50.0%
	GCAAACAAGCTAGGTGGTGA+GGG	-	chr4.1:52033494-52033513	MS.gene51384:CDS	50.0%
	GTGATGGCAAACAAGCTAGG+TGG	-	chr4.1:52033488-52033507	MS.gene51384:CDS	50.0%
!!	CCTAGCTTGTTTGCCATCAC+TGG	+	chr4.1:52033488-52033507	None:intergenic	50.0%
	GGCAAACAAGCTAGGTGGTG+AGG	-	chr4.1:52033493-52033512	MS.gene51384:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	52033455	52033808	52033455	ID=MS.gene51384
chr4.1	mRNA	52033455	52033808	52033455	ID=MS.gene51384.t1;Parent=MS.gene51384
chr4.1	exon	52033455	52033808	52033455	ID=MS.gene51384.t1.exon1;Parent=MS.gene51384.t1
chr4.1	CDS	52033455	52033808	52033455	ID=cds.MS.gene51384.t1;Parent=MS.gene51384.t1

Gene Sequence	>MS.gene51384 ATGGCTACCAATGGTTTTGAAGACTTGTTACCAGTGATGGCAAACAAGCTAGGTGGTGAGGGTTTAATAAAGGAGCTATGCAATGGTTTTGAATTGTTGATGGACAAAGAAAAAGGTGTGATAACATTGGATAGTTTGAGGAAAAACGCTGCTGTTTTGGGTTTACAAGATTTGAAGGAAGATGAACTTGTTGGTATGATGAATGAAGGTGATTTAGATAGAGATGGAGCACTTACTCAGATGGAGTTTTGTGTTTTGATGTTTAGGTTGAGTCCTGAATTGATGGAAGAGTCTTGGTTTTGGCTTGAAGAAGCTCTTCAACATGAACTTGGTGACAACAACTGTTCCTTGTAG
Protein sequence	>MS.gene51384.t1 MATNGFEDLLPVMANKLGGEGLIKELCNGFELLMDKEKGVITLDSLRKNAAVLGLQDLKEDELVGMMNEGDLDRDGALTQMEFCVLMFRLSPELMEESWFWLEEALQHELGDNNCSL