AlfalfaGEDB | MS.gene060610

M. sativa cultivar XinJiangDaYe / MS.gene060610

KEGG: K01369
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0071704 GO:0140096 GO:1901564
PFAM: PF01650.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060610.t1	XP_006599066.1	60.7	61	23	1	2	61	260	320	1.60E-11	78.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060610.t1	P49043	52.5	59	28	0	1	59	271	329	4.0e-11	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060610.t1	K7MFJ7	60.7	61	23	1	2	61	260	320	1.1e-11	78.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060610	MS.gene38444	0.834721	2.54E-56	-1.69E-46
MS.gene060610	MS.gene41507	0.80882	2.57E-50	-1.69E-46
MS.gene060610	MS.gene42887	0.809499	1.84E-50	-1.69E-46
MS.gene060610	MS.gene42891	0.804793	1.82E-49	-1.69E-46
MS.gene060610	MS.gene51252	0.801296	9.63E-49	-1.69E-46
MS.gene060610	MS.gene63142	0.805647	1.21E-49	-1.69E-46
MS.gene060610	MS.gene98673	0.802227	6.20E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060610.t1	MTR_7g032230	61.667	60	18	1	1	60	245	299	2.47e-19	80.9
MS.gene060610.t1	MTR_7g075310	52.632	57	27	0	4	60	260	316	5.52e-14	65.5
MS.gene060610.t1	MTR_3g104120	45.902	61	32	1	1	60	260	320	7.37e-14	65.1
MS.gene060610.t1	MTR_4g101730	44.262	61	32	1	1	59	270	330	2.07e-12	61.2
MS.gene060610.t1	MTR_4g101730	44.262	61	32	1	1	59	270	330	2.07e-12	61.2
MS.gene060610.t1	MTR_7g079140	51.667	60	24	1	4	63	261	315	2.09e-12	61.2
MS.gene060610.t1	MTR_1g016780	42.373	59	33	1	1	59	269	326	1.25e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060610.t1	AT2G25940	46.667	60	31	1	1	59	255	314	1.78e-14	67.0
MS.gene060610.t1	AT4G32940	50.000	60	29	1	1	59	270	329	2.62e-14	66.6
MS.gene060610.t1	AT1G62710	42.623	61	33	1	1	59	262	322	2.24e-11	58.2

Find 12 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGCATTTCTTTGGCTAAC+AGG	0.276050	2.2:+17290146	None:intergenic
AGATTAAATGAGCATTTCTT+TGG	0.329423	2.2:+17290138	None:intergenic
GAGATATGTACTACATTTCA+TGG	0.349654	2.2:-17290557	None:intergenic
AGCTCTCACGTGATGCAATA+TGG	0.372267	2.2:-17290265	MS.gene060610:CDS
CTTTGGCTAACAGGTCTTGT+TGG	0.391530	2.2:+17290155	None:intergenic
GACTGTCTCCTTACATACTT+TGG	0.396857	2.2:-17290223	MS.gene060610:CDS
AGGTCTTGTTGGATTGTTTG+TGG	0.448331	2.2:+17290166	None:intergenic
TGATTGCACCAAAGTATGTA+AGG	0.521428	2.2:+17290215	None:intergenic
TATGTACTACATTTCATGGA+TGG	0.565379	2.2:-17290553	MS.gene060610:CDS
TCAGTAATGTACAGGTCCGT+CGG	0.590240	2.2:-17290312	MS.gene060610:intron
AATCACGCTAATTTCAACAA+TGG	0.645825	2.2:-17290197	MS.gene060610:CDS
GAGTTGCTAAAGTTCTCCGA+CGG	0.672768	2.2:+17290296	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGTTAAATAATGACCAATA+AGG	-	chr2.2:17290185-17290204	MS.gene060610:CDS	20.0%
!!!	TCATTCATTTTCTTATTTTG+TGG	-	chr2.2:17290217-17290236	MS.gene060610:CDS	20.0%
!	AATGATGTTGAATACCTTAT+TGG	+	chr2.2:17290202-17290221	None:intergenic	25.0%
	AATCACGCTAATTTCAACAA+TGG	-	chr2.2:17290496-17290515	MS.gene060610:intron	30.0%
	CATATAAGTATCCACAGATA+AGG	+	chr2.2:17290328-17290347	None:intergenic	30.0%
	CTCAATATTCAGTAATGTAC+AGG	-	chr2.2:17290373-17290392	MS.gene060610:intron	30.0%
	TTCAACTTTATCCTTATCTG+TGG	-	chr2.2:17290314-17290333	MS.gene060610:intron	30.0%
	TCATGGATGGAAGATATGTA+TGG	-	chr2.2:17290153-17290172	MS.gene060610:CDS	35.0%
	TGATTGCACCAAAGTATGTA+AGG	+	chr2.2:17290481-17290500	None:intergenic	35.0%
	GACTGTCTCCTTACATACTT+TGG	-	chr2.2:17290470-17290489	MS.gene060610:intron	40.0%
	TGAGCATTTCTTTGGCTAAC+AGG	+	chr2.2:17290550-17290569	None:intergenic	40.0%
!!	AGGTCTTGTTGGATTGTTTG+TGG	+	chr2.2:17290530-17290549	None:intergenic	40.0%
	AGCTCTCACGTGATGCAATA+TGG	-	chr2.2:17290428-17290447	MS.gene060610:CDS	45.0%
	GAGTTGCTAAAGTTCTCCGA+CGG	+	chr2.2:17290400-17290419	None:intergenic	45.0%
	TCAGTAATGTACAGGTCCGT+CGG	-	chr2.2:17290381-17290400	MS.gene060610:intron	45.0%
!	CTTTGGCTAACAGGTCTTGT+TGG	+	chr2.2:17290541-17290560	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	17290141	17290574	17290141	ID=MS.gene060610
chr2.2	mRNA	17290141	17290574	17290141	ID=MS.gene060610.t1;Parent=MS.gene060610
chr2.2	exon	17290546	17290574	17290546	ID=MS.gene060610.t1.exon1;Parent=MS.gene060610.t1
chr2.2	CDS	17290546	17290574	17290546	ID=cds.MS.gene060610.t1;Parent=MS.gene060610.t1
chr2.2	exon	17290413	17290461	17290413	ID=MS.gene060610.t1.exon2;Parent=MS.gene060610.t1
chr2.2	CDS	17290413	17290461	17290413	ID=cds.MS.gene060610.t1;Parent=MS.gene060610.t1
chr2.2	exon	17290141	17290320	17290141	ID=MS.gene060610.t1.exon3;Parent=MS.gene060610.t1
chr2.2	CDS	17290141	17290320	17290141	ID=cds.MS.gene060610.t1;Parent=MS.gene060610.t1

Gene Sequence

>MS.gene060610

ATGTACTACATTTCATGGATGGAAGATATGTATGGTTTAATGATTTGTTAAATAATGACCAATAAGGTATTCAACATCATTCATTTTCTTATTTTGTGGTTGTTTATGAACAGTGAGAAACATGATCTGACTCAAGAAACTTTGAACGAACAATATGAAACTGTAAGTATCAATTCAACTTTATCCTTATCTGTGGATACTTATATGCATATGAATTCAATTTTGATTTGTACTCAATATTCAGTAATGTACAGGTCCGTCGGAGAACTTTAGCAACTCATGATGCAAGCTCTCACGTGATGCAATATGGAGACATAAATATGAATAAAGACTGTCTCCTTACATACTTTGGTGCAATCACGCTAATTTCAACAATGGCAACAATGCCACAAACAATCCAACAAGACCTGTTAGCCAAAGAAATGCTCATTTAA

Protein sequence

>MS.gene060610.t1

MYYISWMEDIEKHDLTQETLNEQYETVRRRTLATHDASSHVMQYGDINMNKDCLLTYFGAITLISTMATMPQTIQQDLLAKEMLI