Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63142.t1 | GAU45980.1 | 55.9 | 102 | 44 | 1 | 1 | 101 | 524 | 625 | 3.50E-19 | 105.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63142.t1 | A0A2Z6P913 | 55.9 | 102 | 44 | 1 | 1 | 101 | 524 | 625 | 2.5e-19 | 105.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene63142 | 0.804225 | 2.39E-49 | -1.69E-46 |
| MS.gene049853 | MS.gene63142 | 0.83946 | 1.56E-57 | -1.69E-46 |
| MS.gene052447 | MS.gene63142 | 0.827569 | 1.46E-54 | -1.69E-46 |
| MS.gene05329 | MS.gene63142 | 0.821849 | 3.24E-53 | -1.69E-46 |
| MS.gene054174 | MS.gene63142 | 0.800205 | 1.61E-48 | -1.69E-46 |
| MS.gene054175 | MS.gene63142 | 0.803722 | 3.04E-49 | -1.69E-46 |
| MS.gene055017 | MS.gene63142 | 0.820996 | 5.10E-53 | -1.69E-46 |
| MS.gene055058 | MS.gene63142 | 0.804594 | 2.00E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene63142 | 0.81874 | 1.67E-52 | -1.69E-46 |
| MS.gene058580 | MS.gene63142 | 0.83193 | 1.26E-55 | -1.69E-46 |
| MS.gene059276 | MS.gene63142 | 0.806599 | 7.61E-50 | -1.69E-46 |
| MS.gene059458 | MS.gene63142 | 0.810191 | 1.30E-50 | -1.69E-46 |
| MS.gene059460 | MS.gene63142 | 0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene060610 | MS.gene63142 | 0.805647 | 1.21E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63142.t1 | MTR_7g446510 | 72.059 | 68 | 19 | 0 | 37 | 104 | 48 | 115 | 9.61e-26 | 103 |
| MS.gene63142.t1 | MTR_8g468460 | 43.617 | 94 | 51 | 2 | 1 | 93 | 13 | 105 | 8.70e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTGTCATCTTTGTTAGA+TGG | 0.289359 | 8.1:-43574597 | None:intergenic |
| TGTGCTACTTTGGCTTGTTC+AGG | 0.295985 | 8.1:-43574365 | None:intergenic |
| AGTCTCCATGATGCCCTAAC+AGG | 0.397514 | 8.1:-43574419 | None:intergenic |
| CAGCACAGGGGATGAAAACT+TGG | 0.418956 | 8.1:-43573281 | None:intergenic |
| CGTGTGGTGAGGCACCTGTT+AGG | 0.420139 | 8.1:+43574405 | MS.gene63142:CDS |
| ATTGTTCATTGTCGCGACTT+TGG | 0.431904 | 8.1:-43574541 | None:intergenic |
| TGAGGCTGCTGAAATCTATA+CGG | 0.433917 | 8.1:+43573330 | MS.gene63142:CDS |
| AAGCCAAAGTAGCACAGAAA+AGG | 0.490310 | 8.1:+43574372 | MS.gene63142:CDS |
| AGCCAGAAAGACGTGGGAAA+AGG | 0.492341 | 8.1:+43573944 | MS.gene63142:CDS |
| TGGCAGGATAAGAACATTGA+CGG | 0.495176 | 8.1:+43574323 | MS.gene63142:intron |
| TGCAGATGAGTTGTCAGCAC+AGG | 0.496005 | 8.1:-43573295 | None:intergenic |
| GTGAAAGAAGAGATTATGAA+AGG | 0.507624 | 8.1:+43573370 | MS.gene63142:CDS |
| TAGGGCATCATGGAGACTAA+TGG | 0.524044 | 8.1:+43574424 | MS.gene63142:CDS |
| GTGTGGTGAGGCACCTGTTA+GGG | 0.531715 | 8.1:+43574406 | MS.gene63142:CDS |
| TTGACAAAATATATTGAGGA+AGG | 0.543857 | 8.1:+43573448 | MS.gene63142:CDS |
| AGGCACCTGTTAGGGCATCA+TGG | 0.549978 | 8.1:+43574414 | MS.gene63142:CDS |
| GCAGATGAGTTGTCAGCACA+GGG | 0.564043 | 8.1:-43573294 | None:intergenic |
| GAAGTTGACAAAATATATTG+AGG | 0.588592 | 8.1:+43573444 | MS.gene63142:CDS |
| GCATGCTAGCCAGAAAGACG+TGG | 0.591586 | 8.1:+43573937 | MS.gene63142:intron |
| TTGGCTAGCATGATGAGACA+TGG | 0.619223 | 8.1:-43574029 | None:intergenic |
| GAAAGGTGTTGCAATGAGTG+TGG | 0.626883 | 8.1:+43573387 | MS.gene63142:CDS |
| CAAGAAATTTGAATCTCGAG+AGG | 0.629020 | 8.1:+43573522 | MS.gene63142:CDS |
| TCTGCATTGTCTTGAACGTG+AGG | 0.635704 | 8.1:+43573312 | MS.gene63142:CDS |
| GAAAAGAAGAACGTGTGGTG+AGG | 0.652176 | 8.1:+43574394 | MS.gene63142:CDS |
| GGATAAGAACATTGACGGCG+AGG | 0.670157 | 8.1:+43574328 | MS.gene63142:intron |
| AAAAGGAAAAGAAGAACGTG+TGG | 0.672916 | 8.1:+43574389 | MS.gene63142:CDS |
| AGGTGTTGCAATGAGTGTGG+AGG | 0.696599 | 8.1:+43573390 | MS.gene63142:CDS |
| CATGCTAGCCAGAAAGACGT+GGG | 0.697336 | 8.1:+43573938 | MS.gene63142:CDS |
| CAGATGAGTTGTCAGCACAG+GGG | 0.772263 | 8.1:-43573293 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAGAAGTTAATCAAA+AGG | - | chr8.1:43574302-43574321 | None:intergenic | 20.0% |
| !! | AATATTTGATTATATTGCGT+TGG | + | chr8.1:43573601-43573620 | MS.gene63142:intron | 20.0% |
| !! | CCTTCAACAAAAAAAAATAT+AGG | - | chr8.1:43573557-43573576 | None:intergenic | 20.0% |
| !! | CTTAAAAAACAACATATATG+AGG | - | chr8.1:43574491-43574510 | None:intergenic | 20.0% |
| !!! | ACATGGATAATTTTTGATTT+AGG | + | chr8.1:43574515-43574534 | MS.gene63142:intron | 20.0% |
| !!! | CCTATATTTTTTTTTGTTGA+AGG | + | chr8.1:43573554-43573573 | MS.gene63142:intron | 20.0% |
| !!! | CTCATATATGTTGTTTTTTA+AGG | + | chr8.1:43574489-43574508 | MS.gene63142:intron | 20.0% |
| !!! | TTGATTAACTTCTCAATTTT+TGG | + | chr8.1:43574303-43574322 | MS.gene63142:intron | 20.0% |
| ! | ATATATGAGGTCAAGAATAT+GGG | - | chr8.1:43574478-43574497 | None:intergenic | 25.0% |
| ! | CAAAAAAAAATATAGGAGCA+TGG | - | chr8.1:43573550-43573569 | None:intergenic | 25.0% |
| ! | CCAACAATGTGTTTAAATAT+AGG | + | chr8.1:43574239-43574258 | MS.gene63142:intron | 25.0% |
| ! | CCTATATTTAAACACATTGT+TGG | - | chr8.1:43574242-43574261 | None:intergenic | 25.0% |
| ! | CGCAATATAATCAAATATTC+TGG | - | chr8.1:43573600-43573619 | None:intergenic | 25.0% |
| ! | GAAGTTGACAAAATATATTG+AGG | + | chr8.1:43573444-43573463 | MS.gene63142:CDS | 25.0% |
| ! | TTGACAAAATATATTGAGGA+AGG | + | chr8.1:43573448-43573467 | MS.gene63142:CDS | 25.0% |
| !!! | GTTGTTTTTTAAGGTTAACA+TGG | + | chr8.1:43574498-43574517 | MS.gene63142:intron | 25.0% |
| !!! | TGGATAATTTTTGATTTAGG+AGG | + | chr8.1:43574518-43574537 | MS.gene63142:intron | 25.0% |
| CATATATGAGGTCAAGAATA+TGG | - | chr8.1:43574479-43574498 | None:intergenic | 30.0% | |
| GTGAAAGAAGAGATTATGAA+AGG | + | chr8.1:43573370-43573389 | MS.gene63142:CDS | 30.0% | |
| TATATTGCGTTGGACAAAAA+AGG | + | chr8.1:43573611-43573630 | MS.gene63142:intron | 30.0% | |
| TTGTAAGAATGTTCAAACTG+TGG | + | chr8.1:43573645-43573664 | MS.gene63142:intron | 30.0% | |
| !!! | TTAACTTCTCAATTTTTGGC+AGG | + | chr8.1:43574307-43574326 | MS.gene63142:intron | 30.0% |
| AAAAGGAAAAGAAGAACGTG+TGG | + | chr8.1:43574389-43574408 | MS.gene63142:CDS | 35.0% | |
| ACTCTGTCATCTTTGTTAGA+TGG | - | chr8.1:43574600-43574619 | None:intergenic | 35.0% | |
| CAAGAAATTTGAATCTCGAG+AGG | + | chr8.1:43573522-43573541 | MS.gene63142:CDS | 35.0% | |
| GGTACTTACGTTCATATTCA+CGG | - | chr8.1:43574221-43574240 | None:intergenic | 35.0% | |
| !!! | TTTCCTTTTCTGTGCTACTT+TGG | - | chr8.1:43574378-43574397 | None:intergenic | 35.0% |
| AAAGGGATGTAGTTAAAGCG+AGG | + | chr8.1:43574174-43574193 | MS.gene63142:intron | 40.0% | |
| AAGCCAAAGTAGCACAGAAA+AGG | + | chr8.1:43574372-43574391 | MS.gene63142:CDS | 40.0% | |
| AGACGTGGTAGAAGGATTAA+AGG | + | chr8.1:43574156-43574175 | MS.gene63142:intron | 40.0% | |
| ATTGTTCATTGTCGCGACTT+TGG | - | chr8.1:43574544-43574563 | None:intergenic | 40.0% | |
| CAAAGGGAGGAAAATCAAAG+TGG | + | chr8.1:43573766-43573785 | MS.gene63142:intron | 40.0% | |
| CATGATAGCCAAAAAAACGC+GGG | + | chr8.1:43573735-43573754 | MS.gene63142:intron | 40.0% | |
| CATGCTAGCCAAAAAAACGT+GGG | + | chr8.1:43574040-43574059 | MS.gene63142:CDS | 40.0% | |
| GACGTGGTAGAAGGATTAAA+GGG | + | chr8.1:43574157-43574176 | MS.gene63142:intron | 40.0% | |
| GCATGATAGCCAAAAAAACG+CGG | + | chr8.1:43573734-43573753 | MS.gene63142:intron | 40.0% | |
| GTGGAGGAAAAAGAAGTGAA+GGG | + | chr8.1:43573406-43573425 | MS.gene63142:CDS | 40.0% | |
| TAGTTAAAGCGAGGTTGTCA+AGG | + | chr8.1:43574183-43574202 | MS.gene63142:intron | 40.0% | |
| TCATGCTAGCCAAAAAAACG+TGG | + | chr8.1:43574039-43574058 | MS.gene63142:CDS | 40.0% | |
| TGAGGCTGCTGAAATCTATA+CGG | + | chr8.1:43573330-43573349 | MS.gene63142:CDS | 40.0% | |
| TGGCAGGATAAGAACATTGA+CGG | + | chr8.1:43574323-43574342 | MS.gene63142:intron | 40.0% | |
| TGGCTATCATGCTGAGATAT+GGG | - | chr8.1:43573726-43573745 | None:intergenic | 40.0% | |
| TGTGGAGGAAAAAGAAGTGA+AGG | + | chr8.1:43573405-43573424 | MS.gene63142:CDS | 40.0% | |
| TTGGCTATCATGCTGAGATA+TGG | - | chr8.1:43573727-43573746 | None:intergenic | 40.0% | |
| ! | AGTGCTTGCAGAACATTTTC+TGG | - | chr8.1:43573688-43573707 | None:intergenic | 40.0% |
| ! | ATCCTTCTACCACGTCTTTT+TGG | - | chr8.1:43574153-43574172 | None:intergenic | 40.0% |
| ! | ATCCTTCTGCCATGTCTTTT+TGG | - | chr8.1:43573848-43573867 | None:intergenic | 40.0% |
| !! | ATTCTGGAATGTGTTCAACG+CGG | - | chr8.1:43573584-43573603 | None:intergenic | 40.0% |
| !!! | ATCCTTCTCCCACGTTTTTT+TGG | - | chr8.1:43574051-43574070 | None:intergenic | 40.0% |
| AGCCAAAAAAACGTGGGAGA+AGG | + | chr8.1:43574046-43574065 | MS.gene63142:CDS | 45.0% | |
| AGCCAAAAAGACATGGCAGA+AGG | + | chr8.1:43573843-43573862 | MS.gene63142:intron | 45.0% | |
| ATGGCAGAAGGATCATAGAG+AGG | + | chr8.1:43573855-43573874 | MS.gene63142:intron | 45.0% | |
| GAAAAGAAGAACGTGTGGTG+AGG | + | chr8.1:43574394-43574413 | MS.gene63142:CDS | 45.0% | |
| TCTGCATTGTCTTGAACGTG+AGG | + | chr8.1:43573312-43573331 | MS.gene63142:CDS | 45.0% | |
| TGCCAAAAAGACGTGGTAGA+AGG | + | chr8.1:43574148-43574167 | MS.gene63142:intron | 45.0% | |
| TTGGCTAGCATGATGAGACA+TGG | - | chr8.1:43574032-43574051 | None:intergenic | 45.0% | |
| ! | ATCCTTTTCCCACGTCTTTC+TGG | - | chr8.1:43573949-43573968 | None:intergenic | 45.0% |
| ! | TAGGGCATCATGGAGACTAA+TGG | + | chr8.1:43574424-43574443 | MS.gene63142:CDS | 45.0% |
| !! | GAAAGGTGTTGCAATGAGTG+TGG | + | chr8.1:43573387-43573406 | MS.gene63142:CDS | 45.0% |
| !! | TGTGCTACTTTGGCTTGTTC+AGG | - | chr8.1:43574368-43574387 | None:intergenic | 45.0% |
| !!! | ATCCTTCTCCCGCGTTTTTT+TGG | - | chr8.1:43573746-43573765 | None:intergenic | 45.0% |
| AAACGCGGGAGAAGGATCAA+AGG | + | chr8.1:43573749-43573768 | MS.gene63142:intron | 50.0% | |
| AACGCGGGAGAAGGATCAAA+GGG | + | chr8.1:43573750-43573769 | MS.gene63142:intron | 50.0% | |
| AGCCAAAAAAACGCGGGAGA+AGG | + | chr8.1:43573741-43573760 | MS.gene63142:intron | 50.0% | |
| AGCCAGAAAGACGTGGGAAA+AGG | + | chr8.1:43573944-43573963 | MS.gene63142:CDS | 50.0% | |
| AGTCTCCATGATGCCCTAAC+AGG | - | chr8.1:43574422-43574441 | None:intergenic | 50.0% | |
| CAGATGAGTTGTCAGCACAG+GGG | - | chr8.1:43573296-43573315 | None:intergenic | 50.0% | |
| CAGCACAGGGGATGAAAACT+TGG | - | chr8.1:43573284-43573303 | None:intergenic | 50.0% | |
| CATGCTAGCCAGAAAGACGT+GGG | + | chr8.1:43573938-43573957 | MS.gene63142:CDS | 50.0% | |
| GAGTGCTTGCCAAAAAGACG+TGG | + | chr8.1:43574141-43574160 | MS.gene63142:CDS | 50.0% | |
| GCAGATGAGTTGTCAGCACA+GGG | - | chr8.1:43573297-43573316 | None:intergenic | 50.0% | |
| GCCTGCTAGCCAAAAAGACA+TGG | + | chr8.1:43573836-43573855 | MS.gene63142:intron | 50.0% | |
| GGATAAGAACATTGACGGCG+AGG | + | chr8.1:43574328-43574347 | MS.gene63142:intron | 50.0% | |
| TGCAGATGAGTTGTCAGCAC+AGG | - | chr8.1:43573298-43573317 | None:intergenic | 50.0% | |
| TGGCTAGCAGGCTGAGATTT+GGG | - | chr8.1:43573828-43573847 | None:intergenic | 50.0% | |
| TGGCTAGCATGCTGAGACAT+GGG | - | chr8.1:43573929-43573948 | None:intergenic | 50.0% | |
| TTGGCTAGCAGGCTGAGATT+TGG | - | chr8.1:43573829-43573848 | None:intergenic | 50.0% | |
| ! | AGGTGTTGCAATGAGTGTGG+AGG | + | chr8.1:43573390-43573409 | MS.gene63142:CDS | 50.0% |
| !!! | GCCATGTCTTTTTGGCTAGC+AGG | - | chr8.1:43573840-43573859 | None:intergenic | 50.0% |
| CTGGCTAGCATGCTGAGACA+TGG | - | chr8.1:43573930-43573949 | None:intergenic | 55.0% | |
| GCATGCTAGCCAGAAAGACG+TGG | + | chr8.1:43573937-43573956 | MS.gene63142:intron | 55.0% | |
| ! | AGGCACCTGTTAGGGCATCA+TGG | + | chr8.1:43574414-43574433 | MS.gene63142:CDS | 55.0% |
| ! | GTGTGGTGAGGCACCTGTTA+GGG | + | chr8.1:43574406-43574425 | MS.gene63142:CDS | 55.0% |
| GCGGGAGAAGGATCAAAGGG+AGG | + | chr8.1:43573753-43573772 | MS.gene63142:intron | 60.0% | |
| ! | CGTGTGGTGAGGCACCTGTT+AGG | + | chr8.1:43574405-43574424 | MS.gene63142:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 43573238 | 43574623 | 43573238 | ID=MS.gene63142 |
| chr8.1 | mRNA | 43573238 | 43574623 | 43573238 | ID=MS.gene63142.t1;Parent=MS.gene63142 |
| chr8.1 | exon | 43573238 | 43573543 | 43573238 | ID=MS.gene63142.t1.exon1;Parent=MS.gene63142.t1 |
| chr8.1 | CDS | 43573238 | 43573543 | 43573238 | ID=cds.MS.gene63142.t1;Parent=MS.gene63142.t1 |
| chr8.1 | exon | 43573938 | 43574162 | 43573938 | ID=MS.gene63142.t1.exon2;Parent=MS.gene63142.t1 |
| chr8.1 | CDS | 43573938 | 43574162 | 43573938 | ID=cds.MS.gene63142.t1;Parent=MS.gene63142.t1 |
| chr8.1 | exon | 43574329 | 43574454 | 43574329 | ID=MS.gene63142.t1.exon3;Parent=MS.gene63142.t1 |
| chr8.1 | CDS | 43574329 | 43574454 | 43574329 | ID=cds.MS.gene63142.t1;Parent=MS.gene63142.t1 |
| chr8.1 | exon | 43574537 | 43574623 | 43574537 | ID=MS.gene63142.t1.exon4;Parent=MS.gene63142.t1 |
| chr8.1 | CDS | 43574537 | 43574623 | 43574537 | ID=cds.MS.gene63142.t1;Parent=MS.gene63142.t1 |
| Gene Sequence |
| Protein sequence |
