Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51252.t1 | XP_013461632.1 | 87.3 | 158 | 20 | 0 | 1 | 158 | 43 | 200 | 1.30E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51252.t1 | O82368 | 33.9 | 109 | 58 | 5 | 52 | 152 | 203 | 305 | 2.4e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51252.t1 | A0A072V1E7 | 87.3 | 158 | 20 | 0 | 1 | 158 | 43 | 200 | 9.1e-71 | 275.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene51252 | 0.809047 | 2.29E-50 | -1.69E-46 |
| MS.gene049853 | MS.gene51252 | 0.843569 | 1.29E-58 | -1.69E-46 |
| MS.gene052447 | MS.gene51252 | 0.833262 | 5.90E-56 | -1.69E-46 |
| MS.gene054174 | MS.gene51252 | 0.801544 | 8.56E-49 | -1.69E-46 |
| MS.gene054175 | MS.gene51252 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene054484 | MS.gene51252 | 0.806775 | 6.98E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene51252 | 0.829628 | 4.63E-55 | -1.69E-46 |
| MS.gene055056 | MS.gene51252 | 0.80507 | 1.59E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene51252 | 0.820123 | 8.10E-53 | -1.69E-46 |
| MS.gene055952 | MS.gene51252 | 0.802804 | 4.71E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene51252 | 0.83579 | 1.37E-56 | -1.69E-46 |
| MS.gene059458 | MS.gene51252 | 0.81619 | 6.28E-52 | -1.69E-46 |
| MS.gene060610 | MS.gene51252 | 0.801296 | 9.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51252.t1 | MTR_1g106608 | 87.879 | 165 | 12 | 2 | 1 | 158 | 175 | 338 | 9.80e-98 | 285 |
| MS.gene51252.t1 | MTR_1g106600 | 87.879 | 165 | 12 | 2 | 1 | 158 | 175 | 338 | 1.07e-97 | 285 |
| MS.gene51252.t1 | MTR_6g025650 | 87.273 | 165 | 13 | 2 | 1 | 158 | 175 | 338 | 4.42e-97 | 283 |
| MS.gene51252.t1 | MTR_6g018520 | 87.273 | 165 | 13 | 2 | 1 | 158 | 175 | 338 | 4.42e-97 | 283 |
| MS.gene51252.t1 | MTR_3g097540 | 87.342 | 158 | 20 | 0 | 1 | 158 | 43 | 200 | 8.02e-89 | 258 |
| MS.gene51252.t1 | MTR_0002s1260 | 71.429 | 56 | 14 | 1 | 5 | 58 | 70 | 125 | 2.88e-11 | 58.2 |
| MS.gene51252.t1 | MTR_0002s1240 | 71.429 | 56 | 14 | 1 | 5 | 58 | 70 | 125 | 2.88e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGCTGAAGAATAATATT+TGG | 0.182385 | 6.3:-76403894 | MS.gene51252:CDS |
| TCTTGGATAAAATTCAAGTT+AGG | 0.293845 | 6.3:-76403754 | MS.gene51252:CDS |
| TACGGTGGTTCATAGTTATC+TGG | 0.342012 | 6.3:+76404153 | None:intergenic |
| AGAGCTGAAGAATAATATTT+GGG | 0.396765 | 6.3:-76403893 | MS.gene51252:CDS |
| TCCGAGTACGAAAGAAGTTC+TGG | 0.411894 | 6.3:-76404033 | MS.gene51252:CDS |
| AGTGGTGGAATAGGTTGTTT+AGG | 0.419159 | 6.3:+76404117 | None:intergenic |
| TGTCTAACATGATGGAGAAA+AGG | 0.423057 | 6.3:-76403927 | MS.gene51252:CDS |
| CTTGGATAAAATTCAAGTTA+GGG | 0.424184 | 6.3:-76403753 | MS.gene51252:CDS |
| AAAACCTTTCTTCTAGCATT+GGG | 0.433936 | 6.3:-76403975 | MS.gene51252:CDS |
| GAAAACCTTTCTTCTAGCAT+TGG | 0.440421 | 6.3:-76403976 | MS.gene51252:CDS |
| GTCTAACATGATGGAGAAAA+GGG | 0.452368 | 6.3:-76403926 | MS.gene51252:CDS |
| AAAGAAGTTCTGGTGTTGTT+GGG | 0.456237 | 6.3:-76404023 | MS.gene51252:CDS |
| CATCGGAAGACCGCTTGTCT+TGG | 0.467009 | 6.3:-76403771 | MS.gene51252:CDS |
| GAAAGAAGTTCTGGTGTTGT+TGG | 0.468901 | 6.3:-76404024 | MS.gene51252:CDS |
| AATGAAGTCTAAAGCAGATA+AGG | 0.479350 | 6.3:-76403812 | MS.gene51252:CDS |
| TTACCATTATTTGGAATGGA+AGG | 0.482771 | 6.3:-76404213 | None:intergenic |
| CATGATGGAGAAAAGGGAAA+AGG | 0.499421 | 6.3:-76403920 | MS.gene51252:CDS |
| CAACAATCAAGGACCTGCTA+TGG | 0.500995 | 6.3:-76403998 | MS.gene51252:CDS |
| ACCAGAACTTCTTTCGTACT+CGG | 0.509189 | 6.3:+76404032 | None:intergenic |
| GGAATAGGTTGTTTAGGCGA+TGG | 0.519178 | 6.3:+76404123 | None:intergenic |
| TAGTCCCAATGCTAGAAGAA+AGG | 0.534948 | 6.3:+76403971 | None:intergenic |
| ATAGGTTGTTTAGGCGATGG+AGG | 0.535192 | 6.3:+76404126 | None:intergenic |
| GTTGTTTAGGCGATGGAGGT+TGG | 0.536135 | 6.3:+76404130 | None:intergenic |
| TGAAAAGATGTCTAACATGA+TGG | 0.541827 | 6.3:-76403935 | MS.gene51252:CDS |
| TACCATTATTTGGAATGGAA+GGG | 0.543360 | 6.3:-76404212 | None:intergenic |
| TTAGGCGATGGAGGTTGGTA+CGG | 0.556010 | 6.3:+76404135 | None:intergenic |
| GGCGATGGAGGTTGGTACGG+TGG | 0.567657 | 6.3:+76404138 | None:intergenic |
| CCGATGACATCTTCAAGAAG+AGG | 0.571858 | 6.3:+76403788 | None:intergenic |
| GTTGTTGGGATCAACAATCA+AGG | 0.582452 | 6.3:-76404009 | MS.gene51252:CDS |
| CCTCTTCTTGAAGATGTCAT+CGG | 0.587749 | 6.3:-76403788 | MS.gene51252:CDS |
| TTGGATAAAATTCAAGTTAG+GGG | 0.624848 | 6.3:-76403752 | MS.gene51252:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGCTGAAGAATAATATTT+GGG | - | chr6.3:76404049-76404068 | MS.gene51252:CDS | 25.0% |
| !! | CTTGGATAAAATTCAAGTTA+GGG | - | chr6.3:76404189-76404208 | MS.gene51252:CDS | 25.0% |
| !! | TCTTGGATAAAATTCAAGTT+AGG | - | chr6.3:76404188-76404207 | MS.gene51252:CDS | 25.0% |
| !! | TTGGATAAAATTCAAGTTAG+GGG | - | chr6.3:76404190-76404209 | MS.gene51252:CDS | 25.0% |
| AAAACCTTTCTTCTAGCATT+GGG | - | chr6.3:76403967-76403986 | MS.gene51252:CDS | 30.0% | |
| CCTCTGAATCTAAAGAAAAA+TGG | + | chr6.3:76403851-76403870 | None:intergenic | 30.0% | |
| GAATCTAAAGAAAAATGGAG+TGG | + | chr6.3:76403846-76403865 | None:intergenic | 30.0% | |
| GAGAGCTGAAGAATAATATT+TGG | - | chr6.3:76404048-76404067 | MS.gene51252:CDS | 30.0% | |
| TGAAAAGATGTCTAACATGA+TGG | - | chr6.3:76404007-76404026 | MS.gene51252:CDS | 30.0% | |
| ! | AATGAAGTCTAAAGCAGATA+AGG | - | chr6.3:76404130-76404149 | MS.gene51252:CDS | 30.0% |
| ! | TGAATTTTATCCAAGACAAG+CGG | + | chr6.3:76404184-76404203 | None:intergenic | 30.0% |
| !! | CCATTTTTCTTTAGATTCAG+AGG | - | chr6.3:76403848-76403867 | MS.gene51252:CDS | 30.0% |
| !! | CGAGTTTTGTCATCTAAATT+TGG | + | chr6.3:76404092-76404111 | None:intergenic | 30.0% |
| !! | TGACGATTTCGTTTTTCAAT+AGG | + | chr6.3:76403876-76403895 | None:intergenic | 30.0% |
| GAAAACCTTTCTTCTAGCAT+TGG | - | chr6.3:76403966-76403985 | MS.gene51252:CDS | 35.0% | |
| TCTAAAGAAAAATGGAGTGG+TGG | + | chr6.3:76403843-76403862 | None:intergenic | 35.0% | |
| ! | GTCTAACATGATGGAGAAAA+GGG | - | chr6.3:76404016-76404035 | MS.gene51252:CDS | 35.0% |
| ! | TGTCTAACATGATGGAGAAA+AGG | - | chr6.3:76404015-76404034 | MS.gene51252:CDS | 35.0% |
| !! | AAAGAAGTTCTGGTGTTGTT+GGG | - | chr6.3:76403919-76403938 | MS.gene51252:CDS | 35.0% |
| ACCAGAACTTCTTTCGTACT+CGG | + | chr6.3:76403913-76403932 | None:intergenic | 40.0% | |
| CCTCTTCTTGAAGATGTCAT+CGG | - | chr6.3:76404154-76404173 | MS.gene51252:CDS | 40.0% | |
| GTTGTTGGGATCAACAATCA+AGG | - | chr6.3:76403933-76403952 | MS.gene51252:CDS | 40.0% | |
| TACGGTGGTTCATAGTTATC+TGG | + | chr6.3:76403792-76403811 | None:intergenic | 40.0% | |
| TAGTCCCAATGCTAGAAGAA+AGG | + | chr6.3:76403974-76403993 | None:intergenic | 40.0% | |
| ! | AGTGGTGGAATAGGTTGTTT+AGG | + | chr6.3:76403828-76403847 | None:intergenic | 40.0% |
| ! | CATGATGGAGAAAAGGGAAA+AGG | - | chr6.3:76404022-76404041 | MS.gene51252:CDS | 40.0% |
| ! | GAAAAATGGAGTGGTGGAAT+AGG | + | chr6.3:76403837-76403856 | None:intergenic | 40.0% |
| ! | GAAGAAAGGTTTTCCATAGC+AGG | + | chr6.3:76403960-76403979 | None:intergenic | 40.0% |
| !! | GAAAGAAGTTCTGGTGTTGT+TGG | - | chr6.3:76403918-76403937 | MS.gene51252:CDS | 40.0% |
| ATAGGTTGTTTAGGCGATGG+AGG | + | chr6.3:76403819-76403838 | None:intergenic | 45.0% | |
| CAACAATCAAGGACCTGCTA+TGG | - | chr6.3:76403944-76403963 | MS.gene51252:CDS | 45.0% | |
| CCGATGACATCTTCAAGAAG+AGG | + | chr6.3:76404157-76404176 | None:intergenic | 45.0% | |
| GGAATAGGTTGTTTAGGCGA+TGG | + | chr6.3:76403822-76403841 | None:intergenic | 45.0% | |
| TCCGAGTACGAAAGAAGTTC+TGG | - | chr6.3:76403909-76403928 | MS.gene51252:CDS | 45.0% | |
| GTTGTTTAGGCGATGGAGGT+TGG | + | chr6.3:76403815-76403834 | None:intergenic | 50.0% | |
| !! | TTAGGCGATGGAGGTTGGTA+CGG | + | chr6.3:76403810-76403829 | None:intergenic | 50.0% |
| CATCGGAAGACCGCTTGTCT+TGG | - | chr6.3:76404171-76404190 | MS.gene51252:CDS | 55.0% | |
| !! | GGCGATGGAGGTTGGTACGG+TGG | + | chr6.3:76403807-76403826 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 76403744 | 76404220 | 76403744 | ID=MS.gene51252 |
| chr6.3 | mRNA | 76403744 | 76404220 | 76403744 | ID=MS.gene51252.t1;Parent=MS.gene51252 |
| chr6.3 | exon | 76403744 | 76404220 | 76403744 | ID=MS.gene51252.t1.exon1;Parent=MS.gene51252.t1 |
| chr6.3 | CDS | 76403744 | 76404220 | 76403744 | ID=cds.MS.gene51252.t1;Parent=MS.gene51252.t1 |
| Gene Sequence |
| Protein sequence |
