Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062415.t1 | XP_003621906.2 | 94.2 | 137 | 8 | 0 | 1 | 137 | 34 | 170 | 1.50E-64 | 255.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062415.t1 | G7KXV9 | 94.2 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 1.1e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052211 | MS.gene062415 | 0.819601 | 1.07E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene062415 | 0.809404 | 1.92E-50 | -1.69E-46 |
| MS.gene052705 | MS.gene062415 | 0.808292 | 3.33E-50 | -1.69E-46 |
| MS.gene054146 | MS.gene062415 | 0.819539 | 1.10E-52 | -1.69E-46 |
| MS.gene055202 | MS.gene062415 | 0.811238 | 7.74E-51 | -1.69E-46 |
| MS.gene055240 | MS.gene062415 | 0.815764 | 7.82E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene062415 | 0.803029 | 4.24E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene062415 | 0.801881 | 7.31E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene062415 | 0.802997 | 4.30E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene062415 | 0.811575 | 6.54E-51 | -1.69E-46 |
| MS.gene057768 | MS.gene062415 | 0.824875 | 6.37E-54 | -1.69E-46 |
| MS.gene058173 | MS.gene062415 | 0.801781 | 7.66E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene062415 | 0.804264 | 2.35E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene062415 | 0.832878 | 7.35E-56 | -1.69E-46 |
| MS.gene060087 | MS.gene062415 | 0.815666 | 8.22E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062415.t1 | MTR_7g024880 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 3.37e-91 | 260 |
| MS.gene062415.t1 | MTR_7g024890 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 3.84e-91 | 260 |
| MS.gene062415.t1 | MTR_7g024873 | 92.701 | 137 | 10 | 0 | 1 | 137 | 1 | 137 | 6.57e-91 | 259 |
| MS.gene062415.t1 | MTR_8g446810 | 48.571 | 140 | 69 | 1 | 1 | 137 | 1 | 140 | 3.11e-41 | 134 |
| MS.gene062415.t1 | MTR_7g024900 | 90.196 | 51 | 5 | 0 | 1 | 51 | 1 | 51 | 2.87e-26 | 93.6 |
| MS.gene062415.t1 | MTR_1g081270 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 1.58e-19 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062415.t1 | AT2G44310 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 3.22e-39 | 129 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7.3:+75138932 | None:intergenic |
| CTTCTTCATCTTCAAGGAAA+TGG | 0.295262 | 7.3:-75138971 | None:intergenic |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7.3:-75139134 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.342383 | 7.3:-75138977 | None:intergenic |
| CTTGCAATGGCAGATGGGTT+GGG | 0.381320 | 7.3:+75139242 | MS.gene062415:CDS |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7.3:+75139268 | MS.gene062415:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7.3:-75139272 | None:intergenic |
| TTAATAGCTTGGTAGGTAAT+CGG | 0.402120 | 7.3:+75138997 | MS.gene062415:CDS |
| GGGTTTCATGCCAATTCAAA+TGG | 0.441784 | 7.3:+75139262 | MS.gene062415:CDS |
| TGAAGAAGCATTTAATAGCT+TGG | 0.442582 | 7.3:+75138986 | MS.gene062415:CDS |
| CTCCTTCAACATCTCTTCAT+AGG | 0.458078 | 7.3:-75139057 | None:intergenic |
| GCTTGCAATGGCAGATGGGT+TGG | 0.461788 | 7.3:+75139241 | MS.gene062415:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7.3:-75139133 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7.3:+75139073 | MS.gene062415:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7.3:+75139173 | MS.gene062415:CDS |
| CTCCTATGAAGAGATGTTGA+AGG | 0.486473 | 7.3:+75139055 | MS.gene062415:CDS |
| GAAGCATTTAATAGCTTGGT+AGG | 0.494927 | 7.3:+75138990 | MS.gene062415:CDS |
| TTTCGAAAACCCTCAAGCTC+CGG | 0.509695 | 7.3:-75139082 | None:intergenic |
| AACACACTTTGGTATTGATG+TGG | 0.520192 | 7.3:+75139103 | MS.gene062415:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7.3:+75139072 | MS.gene062415:CDS |
| AAGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7.3:+75139063 | MS.gene062415:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7.3:+75139319 | MS.gene062415:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558625 | 7.3:+75139236 | MS.gene062415:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.574792 | 7.3:-75139028 | None:intergenic |
| TTCAAGGAAATGGAAAATTG+TGG | 0.612346 | 7.3:-75138961 | None:intergenic |
| TAAGCAGATGATGCTTGCAA+TGG | 0.622742 | 7.3:+75139229 | MS.gene062415:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.629974 | 7.3:+75139237 | MS.gene062415:CDS |
| CGCCTCGACACAAACAACGA+CGG | 0.676658 | 7.3:+75139026 | MS.gene062415:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7.3:75138980-75138999 | None:intergenic | 30.0% | |
| TTAATAGCTTGGTAGGTAAT+CGG | + | chr7.3:75138997-75139016 | MS.gene062415:CDS | 30.0% | |
| TTCAAGGAAATGGAAAATTG+TGG | - | chr7.3:75138964-75138983 | None:intergenic | 30.0% | |
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7.3:75138936-75138955 | MS.gene062415:CDS | 30.0% |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7.3:75139319-75139338 | MS.gene062415:CDS | 30.0% |
| ! | TGAGTGTAGAAATTTTGGAT+GGG | + | chr7.3:75138937-75138956 | MS.gene062415:CDS | 30.0% |
| !! | TGAAGAAGCATTTAATAGCT+TGG | + | chr7.3:75138986-75139005 | MS.gene062415:CDS | 30.0% |
| CTTCTTCATCTTCAAGGAAA+TGG | - | chr7.3:75138974-75138993 | None:intergenic | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7.3:75139275-75139294 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTTGGT+AGG | + | chr7.3:75138990-75139009 | MS.gene062415:CDS | 35.0% |
| ! | GAGTGTAGAAATTTTGGATG+GGG | + | chr7.3:75138938-75138957 | MS.gene062415:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7.3:75139103-75139122 | MS.gene062415:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7.3:75139307-75139326 | None:intergenic | 35.0% |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7.3:75139173-75139192 | MS.gene062415:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7.3:75139268-75139287 | MS.gene062415:CDS | 40.0% | |
| CTCCTATGAAGAGATGTTGA+AGG | + | chr7.3:75139055-75139074 | MS.gene062415:CDS | 40.0% | |
| CTCCTTCAACATCTCTTCAT+AGG | - | chr7.3:75139060-75139079 | None:intergenic | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7.3:75139262-75139281 | MS.gene062415:CDS | 40.0% | |
| TAAGCAGATGATGCTTGCAA+TGG | + | chr7.3:75139229-75139248 | MS.gene062415:CDS | 40.0% | |
| ! | AGGGTTTTCGAAACACACTT+TGG | + | chr7.3:75139092-75139111 | MS.gene062415:CDS | 40.0% |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7.3:75139136-75139155 | None:intergenic | 45.0% | |
| AAGAGATGTTGAAGGAGCTC+CGG | + | chr7.3:75139063-75139082 | MS.gene062415:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7.3:75139236-75139255 | MS.gene062415:CDS | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7.3:75139137-75139156 | None:intergenic | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7.3:75139237-75139256 | MS.gene062415:CDS | 45.0% | |
| TTTCGAAAACCCTCAAGCTC+CGG | - | chr7.3:75139085-75139104 | None:intergenic | 45.0% | |
| ! | CTTGCAATGGCAGATGGGTT+GGG | + | chr7.3:75139242-75139261 | MS.gene062415:CDS | 50.0% |
| CGCCTCGACACAAACAACGA+CGG | + | chr7.3:75139026-75139045 | MS.gene062415:CDS | 55.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | - | chr7.3:75139031-75139050 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGT+TGG | + | chr7.3:75139241-75139260 | MS.gene062415:CDS | 55.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7.3:75139073-75139092 | MS.gene062415:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7.3:75139072-75139091 | MS.gene062415:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75138936 | 75139349 | 75138936 | ID=MS.gene062415 |
| chr7.3 | mRNA | 75138936 | 75139349 | 75138936 | ID=MS.gene062415.t1;Parent=MS.gene062415 |
| chr7.3 | exon | 75138936 | 75139349 | 75138936 | ID=MS.gene062415.t1.exon1;Parent=MS.gene062415.t1 |
| chr7.3 | CDS | 75138936 | 75139349 | 75138936 | ID=cds.MS.gene062415.t1;Parent=MS.gene062415.t1 |
| Gene Sequence |
| Protein sequence |
