Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062636.t1 | XP_013452629.1 | 96.2 | 365 | 14 | 0 | 1 | 365 | 1 | 365 | 2.60E-204 | 721.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062636.t1 | Q9SZ83 | 54.5 | 363 | 160 | 2 | 1 | 362 | 1 | 359 | 2.1e-117 | 423.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062636.t1 | A0A072UAI0 | 96.2 | 365 | 14 | 0 | 1 | 365 | 1 | 365 | 1.9e-204 | 721.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene062636 | 0.813194 | 2.90E-51 | -1.69E-46 |
| MS.gene049813 | MS.gene062636 | 0.828646 | 8.01E-55 | -1.69E-46 |
| MS.gene052211 | MS.gene062636 | 0.834804 | 2.43E-56 | -1.69E-46 |
| MS.gene052212 | MS.gene062636 | 0.812519 | 4.07E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene062636 | 0.817334 | 3.48E-52 | -1.69E-46 |
| MS.gene053887 | MS.gene062636 | 0.817685 | 2.90E-52 | -1.69E-46 |
| MS.gene054146 | MS.gene062636 | 0.804675 | 1.93E-49 | -1.69E-46 |
| MS.gene05483 | MS.gene062636 | 0.803134 | 4.03E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene062636 | 0.80496 | 1.68E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene062636 | 0.805685 | 1.18E-49 | -1.69E-46 |
| MS.gene059060 | MS.gene062636 | 0.821701 | 3.51E-53 | -1.69E-46 |
| MS.gene059953 | MS.gene062636 | 0.813976 | 1.95E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene062636 | 0.814732 | 1.33E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062636.t1 | MTR_6g066230 | 96.164 | 365 | 14 | 0 | 1 | 365 | 1 | 365 | 0.0 | 734 |
| MS.gene062636.t1 | MTR_6g038640 | 52.486 | 362 | 169 | 2 | 1 | 362 | 1 | 359 | 9.67e-145 | 414 |
| MS.gene062636.t1 | MTR_6g038650 | 51.934 | 362 | 166 | 2 | 1 | 362 | 1 | 354 | 5.22e-142 | 407 |
| MS.gene062636.t1 | MTR_6g038390 | 53.090 | 356 | 164 | 2 | 7 | 362 | 9 | 361 | 4.59e-141 | 405 |
| MS.gene062636.t1 | MTR_1g097250 | 53.039 | 362 | 161 | 3 | 4 | 362 | 3 | 358 | 1.81e-137 | 395 |
| MS.gene062636.t1 | MTR_6g038350 | 53.933 | 356 | 161 | 2 | 7 | 362 | 9 | 361 | 2.88e-135 | 390 |
| MS.gene062636.t1 | MTR_6g038370 | 53.933 | 356 | 161 | 2 | 7 | 362 | 9 | 361 | 2.88e-135 | 390 |
| MS.gene062636.t1 | MTR_3g064900 | 49.727 | 366 | 177 | 4 | 1 | 362 | 1 | 363 | 4.52e-123 | 359 |
| MS.gene062636.t1 | MTR_6g038310 | 63.303 | 109 | 40 | 0 | 7 | 115 | 9 | 117 | 1.79e-38 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062636.t1 | AT4G09670 | 54.545 | 363 | 160 | 2 | 1 | 362 | 1 | 359 | 1.12e-150 | 429 |
| MS.gene062636.t1 | AT1G34200 | 47.922 | 361 | 173 | 3 | 1 | 360 | 1 | 347 | 3.44e-122 | 357 |
| MS.gene062636.t1 | AT1G66130 | 39.782 | 367 | 213 | 6 | 1 | 362 | 1 | 364 | 7.78e-93 | 282 |
Find 84 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTTTGATGTAACAATTT+TGG | 0.095575 | 6.4:+46850778 | MS.gene062636:CDS |
| CCGGAGGTGGTTAGGGTTTA+CGG | 0.200078 | 6.4:+46841328 | MS.gene062636:CDS |
| TCTTCATTAAATGTGGCTTT+TGG | 0.223226 | 6.4:-46850671 | None:intergenic |
| GGGAATGCAAGAACTGATTT+TGG | 0.236717 | 6.4:-46850650 | None:intergenic |
| GGCATTTGGTGCTTTGCTTA+TGG | 0.242579 | 6.4:-46841239 | None:intergenic |
| TCAAGATGTTTGGATGATTA+AGG | 0.243300 | 6.4:+46850961 | MS.gene062636:CDS |
| AGGGGTTGCCGGAGGTGGTT+AGG | 0.272884 | 6.4:+46841320 | MS.gene062636:CDS |
| GGCTAATGGAGTGCAGTTTA+TGG | 0.275677 | 6.4:+46841525 | MS.gene062636:CDS |
| CGGGAGCTATGAGGCTGTTT+TGG | 0.276084 | 6.4:+46841348 | MS.gene062636:CDS |
| ACACATGACGTGGGCTATTA+AGG | 0.281084 | 6.4:+46841417 | MS.gene062636:CDS |
| TGTTGAAATTGTGGTGAATT+AGG | 0.287035 | 6.4:+46851114 | MS.gene062636:CDS |
| GATTAAGGAGTTTGCTGATT+TGG | 0.308124 | 6.4:+46850976 | MS.gene062636:CDS |
| GTTGTGCGGTTTGGTATTCT+TGG | 0.331462 | 6.4:+46841184 | MS.gene062636:CDS |
| CGGAGGTGGTTAGGGTTTAC+GGG | 0.331704 | 6.4:+46841329 | MS.gene062636:CDS |
| TTGCGTGATGAGCAACGTTT+TGG | 0.362561 | 6.4:+46841598 | MS.gene062636:CDS |
| TGCGGCGGAGCAGGGGTTGC+CGG | 0.362721 | 6.4:+46841309 | MS.gene062636:CDS |
| CTTGAAGCTTGTGAGGCTAA+TGG | 0.375758 | 6.4:+46841511 | MS.gene062636:CDS |
| TTAGATGGTCTTGGTGCTCT+TGG | 0.386813 | 6.4:+46850572 | MS.gene062636:CDS |
| CTTGGTGCTCTTGGTGACAT+AGG | 0.392156 | 6.4:+46850581 | MS.gene062636:CDS |
| TCTTCATTACATTGGGAAGA+TGG | 0.417291 | 6.4:+46850716 | MS.gene062636:CDS |
| GGTTGGTAAAATTATGAGGT+TGG | 0.420113 | 6.4:+46850997 | MS.gene062636:CDS |
| GGAGCAGGGGTTGCCGGAGG+TGG | 0.420146 | 6.4:+46841315 | MS.gene062636:CDS |
| ATGTGGTTCTTCATTACATT+GGG | 0.428446 | 6.4:+46850709 | MS.gene062636:CDS |
| TTTGAAGAGAGTTTAGGGTT+TGG | 0.434400 | 6.4:+46850842 | MS.gene062636:CDS |
| GGGGTTGCCGGAGGTGGTTA+GGG | 0.439101 | 6.4:+46841321 | MS.gene062636:CDS |
| AACAAGGTAGGTGGTGTCCT+AGG | 0.439450 | 6.4:+46850906 | MS.gene062636:CDS |
| AAACCAGATTTAGATGGTCT+TGG | 0.445743 | 6.4:+46850563 | MS.gene062636:CDS |
| GGCTATTAAGGCCGCCGAGA+GGG | 0.446692 | 6.4:+46841429 | MS.gene062636:CDS |
| ATTTGGTTGGTAAAATTATG+AGG | 0.447478 | 6.4:+46850993 | MS.gene062636:CDS |
| GCACCAAGACCATCTAAATC+TGG | 0.448639 | 6.4:-46850566 | None:intergenic |
| CATGTGGTTCTTCATTACAT+TGG | 0.455065 | 6.4:+46850708 | MS.gene062636:CDS |
| TTGTTACATCAAAGTTTACA+TGG | 0.457047 | 6.4:-46850772 | None:intergenic |
| TTCAAAGATGGCTGATGAAA+CGG | 0.458307 | 6.4:+46841162 | None:intergenic |
| TTACAGACCATAAAATTGCT+TGG | 0.459459 | 6.4:-46850616 | None:intergenic |
| AACCCTAAACTCTCTTCAAA+AGG | 0.467354 | 6.4:-46850839 | None:intergenic |
| ACAACTGACCATGTCTTCTC+AGG | 0.472529 | 6.4:-46851028 | None:intergenic |
| ATGTGTAAGGCCGTCGGAAG+AGG | 0.474867 | 6.4:-46841400 | None:intergenic |
| ATGGCTATGAGTGTGGCATT+TGG | 0.478499 | 6.4:-46841253 | None:intergenic |
| GCATGTGTTGTTGGAGAAGC+CGG | 0.480611 | 6.4:+46841456 | MS.gene062636:CDS |
| AATAGCCCACGTCATGTGTA+AGG | 0.481701 | 6.4:-46841413 | None:intergenic |
| GATGAAACGGTTGTGCGGTT+TGG | 0.487330 | 6.4:+46841175 | MS.gene062636:CDS |
| AAGGAGTTTGCTGATTTGGT+TGG | 0.495544 | 6.4:+46850980 | MS.gene062636:CDS |
| CCCTAGAACTGCTAAAATGA+AGG | 0.500926 | 6.4:+46841570 | MS.gene062636:CDS |
| TACATTGTCTTCATTAAATG+TGG | 0.510497 | 6.4:-46850678 | None:intergenic |
| CGCATGAAACCAGATTTAGA+TGG | 0.513892 | 6.4:+46850557 | MS.gene062636:CDS |
| TAGAAAGACTCAACTTGTGT+TGG | 0.515492 | 6.4:+46851054 | MS.gene062636:CDS |
| ACTTGTGTTGGATGCTGTGA+AGG | 0.517058 | 6.4:+46851066 | MS.gene062636:CDS |
| TGAATGTGTCGGAGCTTGAT+CGG | 0.521076 | 6.4:+46841485 | MS.gene062636:CDS |
| TTATGAATCTGTTGAAATTG+TGG | 0.535407 | 6.4:+46851105 | MS.gene062636:CDS |
| CGACGGCCTTACACATGACG+TGG | 0.535619 | 6.4:+46841407 | MS.gene062636:CDS |
| CTCTAGAGAACGGCTTCCAA+TGG | 0.536049 | 6.4:-46841272 | None:intergenic |
| GTGCTCTTGGTGACATAGGT+TGG | 0.537363 | 6.4:+46850585 | MS.gene062636:CDS |
| TCCGACACATTCATCGCGAC+CGG | 0.538936 | 6.4:-46841475 | None:intergenic |
| GCTATTAAGGCCGCCGAGAG+GGG | 0.542124 | 6.4:+46841430 | MS.gene062636:CDS |
| CAGTTGCTTTCTCTAGAGAA+CGG | 0.550617 | 6.4:-46841282 | None:intergenic |
| CCGTAAACCCTAACCACCTC+CGG | 0.565216 | 6.4:-46841328 | None:intergenic |
| CTATTAAGGCCGCCGAGAGG+GGG | 0.568298 | 6.4:+46841431 | MS.gene062636:CDS |
| AATGCCACACTCATAGCCAT+TGG | 0.576297 | 6.4:+46841256 | MS.gene062636:CDS |
| ACAAGGTAGGTGGTGTCCTA+GGG | 0.579829 | 6.4:+46850907 | MS.gene062636:CDS |
| ACAACATGTTCATTTGCCCT+AGG | 0.581428 | 6.4:-46850923 | None:intergenic |
| AATGGAGTGCAGTTTATGGA+TGG | 0.581921 | 6.4:+46841529 | MS.gene062636:CDS |
| TCGGATTCTTGAAGCTTGTG+AGG | 0.587102 | 6.4:+46841504 | MS.gene062636:CDS |
| TGTGTAAGGCCGTCGGAAGA+GGG | 0.587524 | 6.4:-46841399 | None:intergenic |
| AAATGAAACCTGAGAAGACA+TGG | 0.592269 | 6.4:+46851020 | MS.gene062636:CDS |
| TGTTACATCAAAGTTTACAT+GGG | 0.596528 | 6.4:-46850771 | None:intergenic |
| TAGGGTTTACGGGAGCTATG+AGG | 0.600628 | 6.4:+46841339 | MS.gene062636:CDS |
| CACGTCATGTGTAAGGCCGT+CGG | 0.603289 | 6.4:-46841406 | None:intergenic |
| CATGGGTTAAGAAAGAGCAA+TGG | 0.609482 | 6.4:-46850754 | None:intergenic |
| AGTTGCATGACATACAATCC+TGG | 0.616144 | 6.4:+46850509 | MS.gene062636:CDS |
| GGCGGAGCAGGGGTTGCCGG+AGG | 0.616283 | 6.4:+46841312 | MS.gene062636:CDS |
| ATGCAAAGATTGAACAAGGT+AGG | 0.623818 | 6.4:+46850894 | MS.gene062636:CDS |
| GGGCTATTAAGGCCGCCGAG+AGG | 0.628969 | 6.4:+46841428 | MS.gene062636:CDS |
| GATTATGCAAAGATTGAACA+AGG | 0.629151 | 6.4:+46850890 | MS.gene062636:CDS |
| GACAATGTAATCATATCATG+TGG | 0.630058 | 6.4:+46850692 | MS.gene062636:CDS |
| GCTTCCAATGGCTATGAGTG+TGG | 0.630480 | 6.4:-46841260 | None:intergenic |
| GCCGGTCGCGATGAATGTGT+CGG | 0.639741 | 6.4:+46841474 | MS.gene062636:CDS |
| TAAGAAAGAGCAATGGAATG+TGG | 0.643558 | 6.4:-46850747 | None:intergenic |
| GTGAAGGAGTCTATTCAAAG+AGG | 0.649981 | 6.4:+46851082 | MS.gene062636:CDS |
| CAAAGATTGAACAAGGTAGG+TGG | 0.653085 | 6.4:+46850897 | MS.gene062636:CDS |
| TAGAACTGCTAAAATGAAGG+AGG | 0.654511 | 6.4:+46841573 | MS.gene062636:CDS |
| AGTCTACATCCCTCTTCCGA+CGG | 0.676976 | 6.4:+46841390 | MS.gene062636:CDS |
| TGGCTGATGAAACGGTTGTG+CGG | 0.695663 | 6.4:+46841170 | MS.gene062636:CDS |
| AGTTTATGGATGGTTCAATG+TGG | 0.704562 | 6.4:+46841539 | MS.gene062636:CDS |
| GACGGCCTTACACATGACGT+GGG | 0.709542 | 6.4:+46841408 | MS.gene062636:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 46841169 | 46851135 | 46841169 | ID=MS.gene062636 |
| chr6.4 | mRNA | 46841169 | 46851135 | 46841169 | ID=MS.gene062636.t1;Parent=MS.gene062636 |
| chr6.4 | exon | 46841169 | 46841633 | 46841169 | ID=MS.gene062636.t1.exon1;Parent=MS.gene062636.t1 |
| chr6.4 | CDS | 46841169 | 46841633 | 46841169 | ID=cds.MS.gene062636.t1;Parent=MS.gene062636.t1 |
| chr6.4 | exon | 46850503 | 46851135 | 46850503 | ID=MS.gene062636.t1.exon2;Parent=MS.gene062636.t1 |
| chr6.4 | CDS | 46850503 | 46851135 | 46850503 | ID=cds.MS.gene062636.t1;Parent=MS.gene062636.t1 |
| Gene Sequence |
| Protein sequence |
