Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057547.t1 | XP_003621334.1 | 93.9 | 98 | 6 | 0 | 1 | 98 | 272 | 369 | 5.60E-45 | 189.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057547.t1 | P93324 | 56.1 | 98 | 43 | 0 | 1 | 98 | 275 | 372 | 2.0e-27 | 122.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057547.t1 | G7L362 | 93.9 | 98 | 6 | 0 | 1 | 98 | 272 | 369 | 4.1e-45 | 189.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene057547 | 0.81155 | 6.62E-51 | -1.69E-46 |
| MS.gene049549 | MS.gene057547 | 0.849708 | 2.72E-60 | -1.69E-46 |
| MS.gene051131 | MS.gene057547 | 0.817643 | 2.96E-52 | -1.69E-46 |
| MS.gene052211 | MS.gene057547 | 0.814513 | 1.48E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene057547 | 0.831959 | 1.24E-55 | -1.69E-46 |
| MS.gene053030 | MS.gene057547 | 0.806518 | 7.91E-50 | -1.69E-46 |
| MS.gene053031 | MS.gene057547 | 0.808255 | 3.39E-50 | -1.69E-46 |
| MS.gene053033 | MS.gene057547 | 0.825339 | 4.95E-54 | -1.69E-46 |
| MS.gene053887 | MS.gene057547 | 0.801432 | 9.03E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene057547 | 0.835125 | 2.01E-56 | -1.69E-46 |
| MS.gene056590 | MS.gene057547 | 0.801584 | 8.41E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene057547 | 0.801137 | 1.04E-48 | -1.69E-46 |
| MS.gene056748 | MS.gene057547 | 0.800088 | 1.70E-48 | -1.69E-46 |
| MS.gene056856 | MS.gene057547 | 0.802138 | 6.47E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene057547 | 0.801646 | 8.16E-49 | -1.69E-46 |
| MS.gene056922 | MS.gene057547 | 0.806263 | 8.96E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene057547 | 0.812176 | 4.84E-51 | -1.69E-46 |
| MS.gene057544 | MS.gene057547 | 0.810933 | 9.01E-51 | -1.69E-46 |
| MS.gene057546 | MS.gene057547 | 0.813011 | 3.18E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene057768 | 0.801361 | 9.34E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene062572 | 0.820727 | 5.89E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene062636 | 0.805685 | 1.18E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene065426 | 0.802122 | 6.52E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene065724 | 0.821536 | 3.83E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene06593 | 0.836788 | 7.62E-57 | -1.69E-46 |
| MS.gene057547 | MS.gene066417 | 0.813181 | 2.91E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene066602 | 0.800726 | 1.26E-48 | -1.69E-46 |
| MS.gene057547 | MS.gene06805 | 0.836259 | 1.04E-56 | -1.69E-46 |
| MS.gene057547 | MS.gene069482 | 0.844584 | 6.91E-59 | -1.69E-46 |
| MS.gene057547 | MS.gene071858 | 0.812212 | 4.75E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene073868 | 0.805443 | 1.33E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene08354 | 0.808525 | 2.97E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene08616 | 0.818791 | 1.63E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene08733 | 0.809392 | 1.94E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene21851 | 0.800682 | 1.29E-48 | -1.69E-46 |
| MS.gene057547 | MS.gene25134 | 0.811837 | 5.73E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene28427 | 0.81484 | 1.26E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene28594 | 0.809922 | 1.49E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene29826 | 0.827525 | 1.49E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene30387 | 0.807262 | 5.51E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene33490 | 0.810578 | 1.07E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene34968 | 0.812108 | 5.01E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene35597 | 0.838665 | 2.51E-57 | -1.69E-46 |
| MS.gene057547 | MS.gene37747 | 0.829235 | 5.77E-55 | -1.69E-46 |
| MS.gene057547 | MS.gene37749 | 0.826872 | 2.14E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene38435 | 0.822423 | 2.39E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene39710 | 0.84618 | 2.55E-59 | -1.69E-46 |
| MS.gene057547 | MS.gene42080 | 0.810012 | 1.42E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene43874 | 0.801883 | 7.30E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene44712 | 0.80131 | 9.56E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene45226 | 0.803922 | 2.77E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene45228 | 0.821114 | 4.80E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene46057 | 0.811589 | 6.49E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene46197 | 0.802519 | 5.40E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene48023 | 0.801499 | 8.75E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene48805 | 0.831284 | 1.82E-55 | -1.69E-46 |
| MS.gene057547 | MS.gene48806 | 0.82185 | 3.24E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene50073 | 0.800948 | 1.13E-48 | -1.69E-46 |
| MS.gene057547 | MS.gene52370 | 0.827717 | 1.34E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene54029 | 0.818357 | 2.04E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene54574 | 0.808396 | 3.16E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene54577 | 0.819322 | 1.23E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene55697 | 0.803224 | 3.86E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene56509 | 0.806135 | 9.53E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene57807 | 0.829832 | 4.13E-55 | -1.69E-46 |
| MS.gene057547 | MS.gene58107 | 0.808346 | 3.24E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene59333 | 0.823266 | 1.52E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene63444 | 0.82521 | 5.31E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene63914 | 0.804258 | 2.35E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene65040 | 0.812151 | 4.90E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene65822 | 0.801677 | 8.04E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene65823 | 0.806413 | 8.33E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene65833 | 0.822866 | 1.88E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene66234 | 0.824541 | 7.63E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene66761 | 0.813 | 3.19E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene68553 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene69966 | 0.806625 | 7.51E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene70061 | 0.821171 | 4.65E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene71346 | 0.800157 | 1.64E-48 | -1.69E-46 |
| MS.gene057547 | MS.gene72073 | 0.804547 | 2.05E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene72503 | 0.808198 | 3.48E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene74774 | 0.816926 | 4.30E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene75472 | 0.818377 | 2.02E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene76539 | 0.803558 | 3.29E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene76738 | 0.827225 | 1.76E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene77033 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene77349 | 0.813931 | 1.99E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene77829 | 0.832463 | 9.32E-56 | -1.69E-46 |
| MS.gene057547 | MS.gene78182 | 0.81424 | 1.70E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene78424 | 0.801333 | 9.46E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene78657 | 0.816531 | 5.27E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene79624 | 0.809351 | 1.97E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene80744 | 0.823343 | 1.46E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene81349 | 0.809852 | 1.54E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene81350 | 0.80148 | 8.83E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene81505 | 0.816696 | 4.84E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene81506 | 0.819327 | 1.23E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene81725 | 0.818647 | 1.76E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene82244 | 0.801681 | 8.03E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene82245 | 0.820454 | 6.80E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene82855 | 0.804081 | 2.56E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene84225 | 0.824693 | 7.03E-54 | -1.69E-46 |
| MS.gene057547 | MS.gene85016 | 0.803338 | 3.66E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene85485 | 0.817183 | 3.76E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene87056 | 0.817843 | 2.67E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene87222 | 0.813254 | 2.81E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene87750 | 0.829423 | 5.19E-55 | -1.69E-46 |
| MS.gene057547 | MS.gene88240 | 0.812013 | 5.25E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene89774 | 0.816317 | 5.88E-52 | -1.69E-46 |
| MS.gene057547 | MS.gene91215 | 0.800493 | 1.40E-48 | -1.69E-46 |
| MS.gene057547 | MS.gene92296 | 0.805173 | 1.52E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene96516 | 0.808641 | 2.80E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene97600 | 0.814977 | 1.17E-51 | -1.69E-46 |
| MS.gene057547 | MS.gene98322 | 0.830882 | 2.29E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057547.t1 | MTR_7g012020 | 93.878 | 98 | 6 | 0 | 1 | 98 | 272 | 369 | 3.43e-63 | 195 |
| MS.gene057547.t1 | MTR_7g011900 | 75.510 | 98 | 24 | 0 | 1 | 98 | 273 | 370 | 1.66e-49 | 160 |
| MS.gene057547.t1 | MTR_7g012070 | 68.041 | 97 | 31 | 0 | 2 | 98 | 279 | 375 | 4.22e-44 | 146 |
| MS.gene057547.t1 | MTR_7g012090 | 65.979 | 97 | 33 | 0 | 2 | 98 | 279 | 375 | 5.52e-43 | 144 |
| MS.gene057547.t1 | MTR_7g011990 | 67.347 | 98 | 32 | 0 | 1 | 98 | 270 | 367 | 3.24e-42 | 141 |
| MS.gene057547.t1 | MTR_7g012040 | 64.948 | 97 | 34 | 0 | 2 | 98 | 272 | 368 | 1.75e-40 | 137 |
| MS.gene057547.t1 | MTR_2g055940 | 63.265 | 98 | 36 | 0 | 1 | 98 | 266 | 363 | 7.22e-40 | 135 |
| MS.gene057547.t1 | MTR_1g096310 | 57.143 | 98 | 42 | 0 | 1 | 98 | 275 | 372 | 2.34e-35 | 124 |
| MS.gene057547.t1 | MTR_3g021430 | 52.041 | 98 | 46 | 1 | 1 | 98 | 261 | 357 | 1.59e-30 | 110 |
| MS.gene057547.t1 | MTR_3g021440 | 46.939 | 98 | 51 | 1 | 1 | 98 | 274 | 370 | 1.02e-27 | 103 |
| MS.gene057547.t1 | MTR_1g036460 | 48.980 | 98 | 49 | 1 | 2 | 98 | 273 | 370 | 1.76e-27 | 102 |
| MS.gene057547.t1 | MTR_1g036490 | 47.959 | 98 | 50 | 1 | 2 | 98 | 273 | 370 | 1.37e-26 | 100 |
| MS.gene057547.t1 | MTR_1g036510 | 43.878 | 98 | 54 | 1 | 2 | 98 | 287 | 384 | 6.96e-24 | 93.6 |
| MS.gene057547.t1 | MTR_7g118300 | 40.625 | 96 | 55 | 2 | 4 | 98 | 281 | 375 | 1.44e-18 | 79.0 |
| MS.gene057547.t1 | MTR_3g092900 | 38.776 | 98 | 58 | 2 | 2 | 98 | 267 | 363 | 4.36e-17 | 74.7 |
| MS.gene057547.t1 | MTR_5g098170 | 36.735 | 98 | 60 | 2 | 2 | 98 | 267 | 363 | 1.71e-16 | 73.2 |
| MS.gene057547.t1 | MTR_1g050220 | 40.260 | 77 | 46 | 0 | 2 | 78 | 208 | 284 | 1.92e-16 | 72.4 |
| MS.gene057547.t1 | MTR_8g024160 | 38.776 | 98 | 58 | 2 | 2 | 98 | 232 | 328 | 4.44e-16 | 71.6 |
| MS.gene057547.t1 | MTR_8g068690 | 39.394 | 99 | 58 | 2 | 1 | 98 | 279 | 376 | 1.03e-15 | 70.9 |
| MS.gene057547.t1 | MTR_8g068760 | 37.755 | 98 | 59 | 2 | 2 | 98 | 137 | 233 | 1.89e-15 | 68.9 |
| MS.gene057547.t1 | MTR_8g068750 | 37.755 | 98 | 59 | 2 | 2 | 98 | 281 | 377 | 5.36e-15 | 68.9 |
| MS.gene057547.t1 | MTR_8g068870 | 38.776 | 98 | 54 | 4 | 4 | 98 | 269 | 363 | 6.49e-15 | 68.6 |
| MS.gene057547.t1 | MTR_0591s0010 | 37.755 | 98 | 55 | 3 | 4 | 98 | 283 | 377 | 1.25e-14 | 67.8 |
| MS.gene057547.t1 | MTR_7g118320 | 35.417 | 96 | 60 | 2 | 4 | 98 | 268 | 362 | 2.77e-14 | 67.0 |
| MS.gene057547.t1 | MTR_8g068730 | 35.714 | 98 | 61 | 2 | 2 | 98 | 249 | 345 | 1.47e-13 | 64.7 |
| MS.gene057547.t1 | MTR_3g096070 | 38.272 | 81 | 48 | 1 | 5 | 83 | 241 | 321 | 3.96e-13 | 63.5 |
| MS.gene057547.t1 | MTR_8g068580 | 36.170 | 94 | 54 | 3 | 8 | 98 | 43 | 133 | 7.08e-13 | 60.5 |
| MS.gene057547.t1 | MTR_6g078850 | 31.959 | 97 | 64 | 2 | 2 | 97 | 287 | 382 | 3.42e-12 | 61.2 |
| MS.gene057547.t1 | MTR_3g096050 | 36.364 | 99 | 54 | 3 | 5 | 98 | 264 | 358 | 1.46e-11 | 59.3 |
| MS.gene057547.t1 | MTR_0079s0070 | 36.782 | 87 | 48 | 4 | 2 | 84 | 238 | 321 | 5.75e-11 | 57.4 |
| MS.gene057547.t1 | MTR_4g038440 | 37.647 | 85 | 46 | 4 | 4 | 84 | 274 | 355 | 8.29e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057547.t1 | AT1G62900 | 38.144 | 97 | 57 | 2 | 4 | 98 | 110 | 205 | 1.86e-20 | 81.6 |
| MS.gene057547.t1 | AT1G21110 | 41.379 | 87 | 49 | 1 | 4 | 88 | 278 | 364 | 2.71e-20 | 83.6 |
| MS.gene057547.t1 | AT1G63140 | 38.144 | 97 | 57 | 2 | 4 | 98 | 306 | 401 | 1.87e-19 | 81.6 |
| MS.gene057547.t1 | AT1G63140 | 38.144 | 97 | 57 | 2 | 4 | 98 | 286 | 381 | 1.99e-19 | 81.3 |
| MS.gene057547.t1 | AT5G54160 | 41.837 | 98 | 55 | 2 | 2 | 98 | 265 | 361 | 2.42e-19 | 80.9 |
| MS.gene057547.t1 | AT1G21100 | 39.080 | 87 | 51 | 1 | 4 | 88 | 278 | 364 | 2.55e-19 | 80.9 |
| MS.gene057547.t1 | AT1G77520 | 37.374 | 99 | 59 | 2 | 2 | 98 | 284 | 381 | 6.64e-19 | 79.7 |
| MS.gene057547.t1 | AT1G21130 | 37.931 | 87 | 52 | 1 | 4 | 88 | 278 | 364 | 1.37e-18 | 79.0 |
| MS.gene057547.t1 | AT1G51990 | 38.776 | 98 | 57 | 2 | 2 | 97 | 265 | 361 | 1.99e-18 | 78.6 |
| MS.gene057547.t1 | AT1G51990 | 38.776 | 98 | 57 | 2 | 2 | 97 | 265 | 361 | 2.08e-18 | 78.6 |
| MS.gene057547.t1 | AT1G21120 | 37.931 | 87 | 52 | 1 | 4 | 88 | 278 | 364 | 5.45e-18 | 77.4 |
| MS.gene057547.t1 | AT1G21120 | 37.931 | 87 | 52 | 1 | 4 | 88 | 326 | 412 | 5.62e-18 | 77.4 |
| MS.gene057547.t1 | AT1G77530 | 37.113 | 97 | 58 | 2 | 4 | 98 | 286 | 381 | 6.70e-18 | 77.0 |
| MS.gene057547.t1 | AT5G53810 | 35.354 | 99 | 61 | 2 | 2 | 98 | 281 | 378 | 7.09e-18 | 77.0 |
| MS.gene057547.t1 | AT1G76790 | 37.374 | 99 | 59 | 2 | 2 | 98 | 268 | 365 | 8.57e-18 | 76.6 |
| MS.gene057547.t1 | AT1G33030 | 39.796 | 98 | 53 | 3 | 4 | 98 | 256 | 350 | 1.18e-15 | 70.9 |
| MS.gene057547.t1 | AT5G37170 | 30.928 | 97 | 64 | 2 | 4 | 98 | 91 | 186 | 5.26e-14 | 64.3 |
| MS.gene057547.t1 | AT5G37170 | 30.928 | 97 | 64 | 2 | 4 | 98 | 139 | 234 | 8.60e-14 | 64.7 |
| MS.gene057547.t1 | AT5G37170 | 30.928 | 97 | 64 | 2 | 4 | 98 | 239 | 334 | 2.98e-13 | 63.9 |
Find 19 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTTGGTTCTAGATCTTC+TGG | 0.261532 | 7.2:-85279771 | None:intergenic |
| TGTGATGGAATATGAGAGTT+TGG | 0.294044 | 7.2:+85279860 | MS.gene057547:CDS |
| TGTCTTGCTTTCTCTATCAT+TGG | 0.299632 | 7.2:+85279918 | MS.gene057547:CDS |
| TTCTAGATCTTCTGGTTGCA+AGG | 0.420081 | 7.2:-85279763 | None:intergenic |
| TACAAAGCTTTGCCACCAAA+TGG | 0.432948 | 7.2:+85279720 | MS.gene057547:CDS |
| TCTAGTCTGTCAGCCATAAT+TGG | 0.441080 | 7.2:+85279661 | None:intergenic |
| GAGAGTTTGGGAAAACGATC+TGG | 0.456073 | 7.2:+85279873 | MS.gene057547:CDS |
| AGACATCTGGAGACATTTGT+TGG | 0.464224 | 7.2:-85279786 | None:intergenic |
| ATCAGCAATTGAAAGACATC+TGG | 0.467294 | 7.2:-85279799 | None:intergenic |
| ATGAGAATGAGCTTTCCATT+TGG | 0.475637 | 7.2:-85279735 | None:intergenic |
| AGAATGAGCTTTCCATTTGG+TGG | 0.491504 | 7.2:-85279732 | None:intergenic |
| TTTCTCTATCATTGGAGTGA+TGG | 0.521287 | 7.2:+85279926 | MS.gene057547:CDS |
| TCTAGATCTTCTGGTTGCAA+GGG | 0.529836 | 7.2:-85279762 | None:intergenic |
| GTGATGGAATATGAGAGTTT+GGG | 0.548540 | 7.2:+85279861 | MS.gene057547:CDS |
| AACATGATGTTTGTCACAGC+TGG | 0.557381 | 7.2:+85279822 | MS.gene057547:CDS |
| GTTTGTCACAGCTGGTGGAA+TGG | 0.572866 | 7.2:+85279830 | MS.gene057547:CDS |
| GTCTGTCAGCCATAATTGGT+CGG | 0.589821 | 7.2:+85279665 | None:intergenic |
| ATGATGTTTGTCACAGCTGG+TGG | 0.606417 | 7.2:+85279825 | MS.gene057547:CDS |
| TGGAATGGAAAGAAGTGTGA+TGG | 0.637051 | 7.2:+85279845 | MS.gene057547:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAGCAATTGAAAGACATC+TGG | - | chr7.2:85279802-85279821 | None:intergenic | 35.0% | |
| ATGAGAATGAGCTTTCCATT+TGG | - | chr7.2:85279738-85279757 | None:intergenic | 35.0% | |
| TGTCTTGCTTTCTCTATCAT+TGG | + | chr7.2:85279918-85279937 | MS.gene057547:CDS | 35.0% | |
| TTTCTCTATCATTGGAGTGA+TGG | + | chr7.2:85279926-85279945 | MS.gene057547:CDS | 35.0% | |
| ! | ACTTTTCATCCGACCAATTA+TGG | - | chr7.2:85279677-85279696 | None:intergenic | 35.0% |
| ! | GTGATGGAATATGAGAGTTT+GGG | + | chr7.2:85279861-85279880 | MS.gene057547:CDS | 35.0% |
| ! | TGTGATGGAATATGAGAGTT+TGG | + | chr7.2:85279860-85279879 | MS.gene057547:CDS | 35.0% |
| !! | TTTGTTGGTTCTAGATCTTC+TGG | - | chr7.2:85279774-85279793 | None:intergenic | 35.0% |
| AACATGATGTTTGTCACAGC+TGG | + | chr7.2:85279822-85279841 | MS.gene057547:CDS | 40.0% | |
| AGAATGAGCTTTCCATTTGG+TGG | - | chr7.2:85279735-85279754 | None:intergenic | 40.0% | |
| AGACATCTGGAGACATTTGT+TGG | - | chr7.2:85279789-85279808 | None:intergenic | 40.0% | |
| TACAAAGCTTTGCCACCAAA+TGG | + | chr7.2:85279720-85279739 | MS.gene057547:CDS | 40.0% | |
| ! | TCTAGATCTTCTGGTTGCAA+GGG | - | chr7.2:85279765-85279784 | None:intergenic | 40.0% |
| ! | TGGAATGGAAAGAAGTGTGA+TGG | + | chr7.2:85279845-85279864 | MS.gene057547:CDS | 40.0% |
| ! | TTCTAGATCTTCTGGTTGCA+AGG | - | chr7.2:85279766-85279785 | None:intergenic | 40.0% |
| ATGATGTTTGTCACAGCTGG+TGG | + | chr7.2:85279825-85279844 | MS.gene057547:CDS | 45.0% | |
| GAGAGTTTGGGAAAACGATC+TGG | + | chr7.2:85279873-85279892 | MS.gene057547:CDS | 45.0% | |
| GTTTGTCACAGCTGGTGGAA+TGG | + | chr7.2:85279830-85279849 | MS.gene057547:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 85279666 | 85279962 | 85279666 | ID=MS.gene057547 |
| chr7.2 | mRNA | 85279666 | 85279962 | 85279666 | ID=MS.gene057547.t1;Parent=MS.gene057547 |
| chr7.2 | exon | 85279666 | 85279962 | 85279666 | ID=MS.gene057547.t1.exon1;Parent=MS.gene057547.t1 |
| chr7.2 | CDS | 85279666 | 85279962 | 85279666 | ID=cds.MS.gene057547.t1;Parent=MS.gene057547.t1 |
| Gene Sequence |
| Protein sequence |
