AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052212


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052212.t1 MTR_3g087280 86.432 199 27 0 1 199 1 199 1.94e-131 367
MS.gene052212.t1 MTR_3g087325 86.432 199 27 0 1 199 1 199 2.15e-128 359
MS.gene052212.t1 MTR_3g087278 84.925 199 30 0 1 199 1 199 2.22e-128 359
MS.gene052212.t1 MTR_3g087275 86.432 199 26 1 1 199 1 198 1.54e-126 355
MS.gene052212.t1 MTR_3g087270 83.417 199 33 0 1 199 1 199 7.77e-125 350
MS.gene052212.t1 MTR_3g087330 81.910 199 36 0 1 199 1 199 9.06e-124 348
MS.gene052212.t1 MTR_3g087300 67.513 197 20 3 1 197 1 153 3.22e-87 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052212.t1 AT5G65030 31.472 197 110 7 11 187 10 201 3.44e-25 97.4
MS.gene052212.t1 AT5G10210 31.313 198 110 8 15 199 13 197 1.17e-24 95.9

Find 36 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCCAAATCATTGCCCTTT+TGG 0.190841 3.2:-74504811 MS.gene052212:CDS
ACACTAGGCTTTGTTGATTA+AGG 0.234215 3.2:+74504738 None:intergenic
CTCACCTTTCCCAATTAGTT+TGG 0.258127 3.2:+74504685 None:intergenic
ATATGGAGCTCCAAACTAAT+TGG 0.259330 3.2:-74504695 MS.gene052212:CDS
TTAATCTCCACTTCCACTTC+TGG 0.281665 3.2:+74504557 None:intergenic
TGCACTTGGCGCAGCTTTGG+AGG 0.390376 3.2:-74504405 MS.gene052212:CDS
GTCCCAAAAGGGCAATGATT+TGG 0.392085 3.2:+74504808 None:intergenic
ATTTGCACTTGGCGCAGCTT+TGG 0.400082 3.2:-74504408 MS.gene052212:CDS
AGCTAAGGCAAAGGAAGATA+TGG 0.410362 3.2:-74504712 MS.gene052212:CDS
GATTCAAGAATCACTTTCCA+TGG 0.437928 3.2:+74504659 None:intergenic
TATGGAGCTCCAAACTAATT+GGG 0.458287 3.2:-74504694 MS.gene052212:CDS
AAAATGGTTGAAGATGGATT+TGG 0.470551 3.2:-74504618 MS.gene052212:CDS
AGTAGCTTCGGTTGCTGAGA+TGG 0.471131 3.2:-74504528 MS.gene052212:CDS
GATTAAAATAAAAGTAGCTT+CGG 0.474275 3.2:-74504540 MS.gene052212:CDS
ACTAAATGACTCGTCCCAAA+AGG 0.477554 3.2:+74504796 None:intergenic
CTGTGAATTGAGAATAATAC+AGG 0.480110 3.2:-74504936 MS.gene052212:CDS
TTGGGAAAGGTGAGATTCCA+TGG 0.490587 3.2:-74504676 MS.gene052212:CDS
AAAGCCTAGTGTTGGAGCTA+AGG 0.499139 3.2:-74504727 MS.gene052212:CDS
TTTGCCTTAGCTCCAACACT+AGG 0.507447 3.2:+74504723 None:intergenic
AGATGGAGAAGCAAAATAAG+AGG 0.509817 3.2:-74504511 MS.gene052212:CDS
CTAAATGACTCGTCCCAAAA+GGG 0.528554 3.2:+74504797 None:intergenic
ATGAATAATTTGAATGAGTG+TGG 0.533229 3.2:-74504485 MS.gene052212:CDS
ACGGCCATGACCATCGTGCT+TGG 0.538014 3.2:-74504454 MS.gene052212:CDS
CCAAAAGGGCAATGATTTGG+CGG 0.551334 3.2:+74504811 None:intergenic
TAATCAACAAAGCCTAGTGT+TGG 0.564525 3.2:-74504735 MS.gene052212:CDS
AGCACCAAGCACGATGGTCA+TGG 0.566327 3.2:+74504450 None:intergenic
AGTCGAAGTGATTGCAAAGA+GGG 0.568276 3.2:-74504593 MS.gene052212:CDS
CATAACGCCAGAAGTGGAAG+TGG 0.570407 3.2:-74504564 MS.gene052212:CDS
GATTCTTGAATCACAAAACA+TGG 0.583125 3.2:-74504648 MS.gene052212:CDS
TATGTTCATAACGCCAGAAG+TGG 0.583659 3.2:-74504570 MS.gene052212:CDS
ACATATGAAGATACATGAGA+TGG 0.585374 3.2:+74504968 None:intergenic
TAGTGTTGGAGCTAAGGCAA+AGG 0.590191 3.2:-74504721 MS.gene052212:CDS
GATTGTGATATATTTGCACT+TGG 0.591421 3.2:-74504419 MS.gene052212:CDS
AGCTCCAAACTAATTGGGAA+AGG 0.607689 3.2:-74504689 MS.gene052212:CDS
GAGTCGAAGTGATTGCAAAG+AGG 0.661989 3.2:-74504594 MS.gene052212:CDS
CAGCGCAGCACCAAGCACGA+TGG 0.681591 3.2:+74504444 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAAAATAAAAGTAGCTT+CGG - chr3.2:74504831-74504850 MS.gene052212:CDS 20.0%
! AAAACATGGAGCTAAAAAAA+TGG - chr3.2:74504737-74504756 MS.gene052212:CDS 25.0%
! ATGAATAATTTGAATGAGTG+TGG - chr3.2:74504886-74504905 MS.gene052212:CDS 25.0%
ACATATGAAGATACATGAGA+TGG + chr3.2:74504406-74504425 None:intergenic 30.0%
CTGTGAATTGAGAATAATAC+AGG - chr3.2:74504435-74504454 MS.gene052212:CDS 30.0%
GATTCTTGAATCACAAAACA+TGG - chr3.2:74504723-74504742 MS.gene052212:CDS 30.0%
GATTGTGATATATTTGCACT+TGG - chr3.2:74504952-74504971 MS.gene052212:CDS 30.0%
GCTAAAAAAATGGTTGAAGA+TGG - chr3.2:74504747-74504766 MS.gene052212:CDS 30.0%
! AAAATGGTTGAAGATGGATT+TGG - chr3.2:74504753-74504772 MS.gene052212:CDS 30.0%
! AAGGTTTTCCATGAAATCTT+GGG + chr3.2:74504617-74504636 None:intergenic 30.0%
! TAAGGTTTTCCATGAAATCT+TGG + chr3.2:74504618-74504637 None:intergenic 30.0%
!!! ATTCTTTTGTTGTTCTCTGT+TGG + chr3.2:74504517-74504536 None:intergenic 30.0%
AATGAGTGTGGATGCAAAAA+CGG - chr3.2:74504898-74504917 MS.gene052212:CDS 35.0%
AGATGGAGAAGCAAAATAAG+AGG - chr3.2:74504860-74504879 MS.gene052212:CDS 35.0%
ATATGGAGCTCCAAACTAAT+TGG - chr3.2:74504676-74504695 MS.gene052212:CDS 35.0%
GATTCAAGAATCACTTTCCA+TGG + chr3.2:74504715-74504734 None:intergenic 35.0%
TAATCAACAAAGCCTAGTGT+TGG - chr3.2:74504636-74504655 MS.gene052212:CDS 35.0%
TATGGAGCTCCAAACTAATT+GGG - chr3.2:74504677-74504696 MS.gene052212:CDS 35.0%
! AGGTTTTCCATGAAATCTTG+GGG + chr3.2:74504616-74504635 None:intergenic 35.0%
!! ACACTAGGCTTTGTTGATTA+AGG + chr3.2:74504636-74504655 None:intergenic 35.0%
ACTAAATGACTCGTCCCAAA+AGG + chr3.2:74504578-74504597 None:intergenic 40.0%
AGCTAAGGCAAAGGAAGATA+TGG - chr3.2:74504659-74504678 MS.gene052212:CDS 40.0%
AGCTCCAAACTAATTGGGAA+AGG - chr3.2:74504682-74504701 MS.gene052212:CDS 40.0%
AGTCGAAGTGATTGCAAAGA+GGG - chr3.2:74504778-74504797 MS.gene052212:CDS 40.0%
CTAAATGACTCGTCCCAAAA+GGG + chr3.2:74504577-74504596 None:intergenic 40.0%
CTCACCTTTCCCAATTAGTT+TGG + chr3.2:74504689-74504708 None:intergenic 40.0%
TATGTTCATAACGCCAGAAG+TGG - chr3.2:74504801-74504820 MS.gene052212:CDS 40.0%
TTAATCTCCACTTCCACTTC+TGG + chr3.2:74504817-74504836 None:intergenic 40.0%
! CGACGAAAAACTTGTTTGCA+AGG - chr3.2:74504482-74504501 MS.gene052212:CDS 40.0%
! GGTTTTCCATGAAATCTTGG+GGG + chr3.2:74504615-74504634 None:intergenic 40.0%
GAGTCGAAGTGATTGCAAAG+AGG - chr3.2:74504777-74504796 MS.gene052212:CDS 45.0%
GTCCCAAAAGGGCAATGATT+TGG + chr3.2:74504566-74504585 None:intergenic 45.0%
TTCTTGCCCCCAAGATTTCA+TGG - chr3.2:74504606-74504625 MS.gene052212:CDS 45.0%
TTGGGAAAGGTGAGATTCCA+TGG - chr3.2:74504695-74504714 MS.gene052212:CDS 45.0%
TTTGCCTTAGCTCCAACACT+AGG + chr3.2:74504651-74504670 None:intergenic 45.0%
! AAAGCCTAGTGTTGGAGCTA+AGG - chr3.2:74504644-74504663 MS.gene052212:CDS 45.0%
! CCAAAAGGGCAATGATTTGG+CGG + chr3.2:74504563-74504582 None:intergenic 45.0%
! CGCCAAATCATTGCCCTTTT+GGG - chr3.2:74504561-74504580 MS.gene052212:CDS 45.0%
! TAGTGTTGGAGCTAAGGCAA+AGG - chr3.2:74504650-74504669 MS.gene052212:CDS 45.0%
AGTAGCTTCGGTTGCTGAGA+TGG - chr3.2:74504843-74504862 MS.gene052212:CDS 50.0%
ATTTGCACTTGGCGCAGCTT+TGG - chr3.2:74504963-74504982 MS.gene052212:CDS 50.0%
CATAACGCCAGAAGTGGAAG+TGG - chr3.2:74504807-74504826 MS.gene052212:CDS 50.0%
! CCGCCAAATCATTGCCCTTT+TGG - chr3.2:74504560-74504579 MS.gene052212:CDS 50.0%
AGCACCAAGCACGATGGTCA+TGG + chr3.2:74504924-74504943 None:intergenic 55.0%
ACGGCCATGACCATCGTGCT+TGG - chr3.2:74504917-74504936 MS.gene052212:CDS 60.0%
! TGCACTTGGCGCAGCTTTGG+AGG - chr3.2:74504966-74504985 MS.gene052212:CDS 60.0%
CAGCGCAGCACCAAGCACGA+TGG + chr3.2:74504930-74504949 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74504397 74504996 74504397 ID=MS.gene052212
chr3.2 mRNA 74504397 74504996 74504397 ID=MS.gene052212.t1;Parent=MS.gene052212
chr3.2 exon 74504397 74504996 74504397 ID=MS.gene052212.t1.exon1;Parent=MS.gene052212.t1
chr3.2 CDS 74504397 74504996 74504397 ID=cds.MS.gene052212.t1;Parent=MS.gene052212.t1
Gene Sequence

>MS.gene052212

ATGGATCCATCTCATGTATCTTCATATGTAAACTTCAGCTGTGAATTGAGAATAATACAGGCTCGAAACATTGAATTCATCAAGACGACGAAAAACTTGTTTGCAAGGTTGTATCTTCCAACAGAGAACAACAAAAGAATTCAACTCAACAGCAAAAATGTTTCCGCCAAATCATTGCCCTTTTGGGACGAGTCATTTAGTCTAGATTGTTCTTGCCCCCAAGATTTCATGGAAAACCTTAATCAACAAAGCCTAGTGTTGGAGCTAAGGCAAAGGAAGATATGGAGCTCCAAACTAATTGGGAAAGGTGAGATTCCATGGAAAGTGATTCTTGAATCACAAAACATGGAGCTAAAAAAATGGTTGAAGATGGATTTGGTGAGTCGAAGTGATTGCAAAGAGGGTATGTTCATAACGCCAGAAGTGGAAGTGGAGATTAAAATAAAAGTAGCTTCGGTTGCTGAGATGGAGAAGCAAAATAAGAGGAGAATGAATAATTTGAATGAGTGTGGATGCAAAAACGGCCATGACCATCGTGCTTGGTGCTGCGCTGAAGATTGTGATATATTTGCACTTGGCGCAGCTTTGGAGGCTTTTTAA

Protein sequence

>MS.gene052212.t1

MDPSHVSSYVNFSCELRIIQARNIEFIKTTKNLFARLYLPTENNKRIQLNSKNVSAKSLPFWDESFSLDCSCPQDFMENLNQQSLVLELRQRKIWSSKLIGKGEIPWKVILESQNMELKKWLKMDLVSRSDCKEGMFITPEVEVEIKIKVASVAEMEKQNKRRMNNLNECGCKNGHDHRAWCCAEDCDIFALGAALEAF