AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062651


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062651.t1 MTR_6g065460 90.865 208 18 1 1 208 1 207 6.23e-129 362
MS.gene062651.t1 MTR_6g065460 81.250 208 14 1 1 208 1 183 1.25e-108 309
MS.gene062651.t1 MTR_6g059650 67.380 187 51 6 19 202 20 199 1.80e-78 234
MS.gene062651.t1 MTR_3g463590 54.450 191 78 4 27 213 26 211 3.23e-62 193
MS.gene062651.t1 MTR_6g059530 51.163 215 91 6 1 213 1 203 1.32e-55 176
MS.gene062651.t1 MTR_6g059410 50.926 216 89 8 1 213 1 202 6.23e-53 169
MS.gene062651.t1 MTR_6g059760 49.321 221 96 10 1 213 1 213 8.68e-53 169
MS.gene062651.t1 MTR_6g059730 50.739 203 94 5 6 206 7 205 4.14e-50 162
MS.gene062651.t1 MTR_6g045097 43.243 222 112 8 1 213 1 217 2.88e-47 155
MS.gene062651.t1 MTR_6g044780 42.396 217 114 7 1 211 1 212 3.73e-47 155
MS.gene062651.t1 MTR_6g044810 42.396 217 114 7 1 211 1 212 4.63e-47 154
MS.gene062651.t1 MTR_6g017225 47.596 208 95 7 6 205 7 208 2.55e-45 154
MS.gene062651.t1 MTR_6g045403 41.860 215 116 5 1 211 1 210 2.79e-45 150
MS.gene062651.t1 MTR_6g059680 49.246 199 91 6 15 207 16 210 3.13e-44 147
MS.gene062651.t1 MTR_6g045433 38.914 221 112 6 7 213 8 219 1.63e-39 135
MS.gene062651.t1 MTR_6g009650 41.784 213 111 7 1 208 1 205 7.97e-38 130
MS.gene062651.t1 MTR_6g066190 41.784 213 109 8 1 207 1 204 1.06e-37 130
MS.gene062651.t1 MTR_6g059810 42.584 209 102 10 4 202 8 208 4.49e-37 129
MS.gene062651.t1 MTR_6g066210 41.038 212 114 6 1 207 1 206 1.19e-36 127
MS.gene062651.t1 MTR_6g009563 40.000 215 113 8 1 208 1 206 1.25e-33 120
MS.gene062651.t1 MTR_1g035770 39.394 198 109 7 6 195 7 201 4.29e-32 116
MS.gene062651.t1 MTR_6g065570 79.365 63 13 0 14 76 11 73 4.17e-30 107
MS.gene062651.t1 MTR_6g059755 50.000 124 52 6 96 213 11 130 1.97e-29 106
MS.gene062651.t1 MTR_1g057110 36.279 215 100 11 1 206 1 187 9.92e-27 101
MS.gene062651.t1 MTR_6g078290 34.722 216 120 9 1 202 1 209 7.34e-24 94.7
MS.gene062651.t1 MTR_6g078100 36.744 215 116 11 1 205 1 205 1.34e-23 94.0
MS.gene062651.t1 MTR_6g078070 34.884 215 118 11 1 205 1 203 6.47e-23 92.0
MS.gene062651.t1 MTR_6g078140 32.000 200 114 8 21 205 21 213 1.52e-17 78.2
MS.gene062651.t1 MTR_6g078120 32.000 200 114 8 21 205 21 213 3.99e-17 77.0
MS.gene062651.t1 MTR_3g106585 33.180 217 115 12 5 211 7 203 1.06e-15 73.2
MS.gene062651.t1 MTR_3g106620 30.108 186 111 8 28 206 27 200 3.82e-15 71.6
MS.gene062651.t1 MTR_2g073010 28.713 202 128 7 7 205 10 198 7.58e-14 67.8
MS.gene062651.t1 MTR_0302s0030 28.358 201 135 6 7 202 3 199 1.09e-12 64.7
MS.gene062651.t1 MTR_3g014870 28.293 205 127 8 5 202 9 200 2.09e-12 63.9
MS.gene062651.t1 MTR_6g078280 29.293 198 122 9 21 205 20 212 3.38e-12 63.5
MS.gene062651.t1 MTR_3g014820 27.805 205 128 8 5 202 9 200 1.28e-11 61.6
MS.gene062651.t1 MTR_0084s0030 27.861 201 136 6 7 202 3 199 1.36e-11 61.6
MS.gene062651.t1 MTR_2g072980 28.141 199 134 7 7 205 10 199 4.53e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062651.t1 AT1G17860 33.824 204 122 8 4 205 3 195 2.17e-21 87.8
MS.gene062651.t1 AT1G73260 31.579 209 130 7 1 206 8 206 2.95e-20 85.5
MS.gene062651.t1 AT1G73325 30.583 206 130 7 4 205 7 203 1.38e-16 75.5

Find 62 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATCATGCCATCAATCTT+TGG 0.296622 6.4:+46272576 MS.gene062651:CDS
GGGAGGCATGATGACTTTAC+TGG 0.309229 6.4:+46272981 MS.gene062651:CDS
CCTATCCTGCTAAACTTTAC+AGG 0.312183 6.4:-46272693 None:intergenic
CCTGGTGCTTGTTATGATAT+TGG 0.331841 6.4:+46272960 MS.gene062651:CDS
TGACTTTACTGGAAGGCTTC+TGG 0.336007 6.4:+46272992 MS.gene062651:CDS
CTGGTGCTTGTTATGATATT+GGG 0.341631 6.4:+46272961 MS.gene062651:CDS
GGTAAGGAATGTAGGTTTCA+TGG 0.342364 6.4:-46272442 None:intergenic
TACTGGAAGGCTTCTGGTCC+TGG 0.347355 6.4:+46272998 MS.gene062651:CDS
GAAGGTAAGCATATTATAGA+TGG 0.366110 6.4:+46272867 MS.gene062651:CDS
TTTGTTGATGCCTCTCAAAA+TGG 0.385354 6.4:+46273047 MS.gene062651:CDS
ATCAACAAATACAACTTCAT+AGG 0.388203 6.4:-46273033 None:intergenic
CCAGGTGGGGCAGTGATAGA+TGG 0.414356 6.4:-46272942 None:intergenic
CCAGCTGCTCCAAAGATTGA+TGG 0.422912 6.4:-46272585 None:intergenic
ATCTTTGGAGCAGCTGGTGG+TGG 0.441234 6.4:+46272591 MS.gene062651:CDS
GAACAAGTTATTGACACAAA+TGG 0.443270 6.4:+46272525 MS.gene062651:CDS
CCATCTATCACTGCCCCACC+TGG 0.453523 6.4:+46272942 MS.gene062651:CDS
CCATCAATCTTTGGAGCAGC+TGG 0.460514 6.4:+46272585 MS.gene062651:CDS
ACTGGACCATGGGTGGGTAT+TGG 0.466256 6.4:+46272828 MS.gene062651:CDS
GAGCAGCTGGTGGTGGATTG+AGG 0.467057 6.4:+46272598 MS.gene062651:CDS
CTTGGAGGATTCAGCACACT+CGG 0.472541 6.4:-46272775 None:intergenic
GTCTGCCTGTAAAGTTTAGC+AGG 0.475302 6.4:+46272688 MS.gene062651:CDS
AAAGAAAGGGTAAGGAATGT+AGG 0.478598 6.4:-46272450 None:intergenic
GGACCATGGGTGGGTATTGG+TGG 0.483131 6.4:+46272831 MS.gene062651:CDS
TGGACAGAACACAAACTTGT+AGG 0.486171 6.4:-46272922 None:intergenic
GATTATTCCGAGGTTGTCAA+TGG 0.494338 6.4:+46272666 MS.gene062651:CDS
CATAGGGATCATTATTAGCC+AGG 0.494704 6.4:-46273016 None:intergenic
CCTGTAAAGTTTAGCAGGAT+AGG 0.495257 6.4:+46272693 MS.gene062651:CDS
TAACAAATTAACTGGACCAT+GGG 0.499496 6.4:+46272818 MS.gene062651:CDS
ATGCAAAGAGGAGCAAAGAA+AGG 0.504725 6.4:-46272464 None:intergenic
CAAATTAACTGGACCATGGG+TGG 0.513850 6.4:+46272821 MS.gene062651:CDS
TAATCTTGAAGTACAGTAAG+TGG 0.514474 6.4:-46272648 None:intergenic
AAGTTTAGCAGGATAGGTGC+AGG 0.518079 6.4:+46272699 MS.gene062651:CDS
TGCAAAAGCTAATGAAAAGC+AGG 0.523206 6.4:-46272493 None:intergenic
CTTGTAAATGCGATATCAAG+TGG 0.537798 6.4:-46272747 None:intergenic
ATCATCAACCAACACCCACT+TGG 0.538112 6.4:-46272793 None:intergenic
AGAGGAGCAAAGAAAGGGTA+AGG 0.541291 6.4:-46272458 None:intergenic
TCAACAAATACAACTTCATA+GGG 0.541440 6.4:-46273032 None:intergenic
TGCAAAGAGGAGCAAAGAAA+GGG 0.546163 6.4:-46272463 None:intergenic
ATCAACCAACACCCACTTGG+AGG 0.563736 6.4:-46272790 None:intergenic
TGAATCCTCCAAGTGGGTGT+TGG 0.564748 6.4:+46272785 MS.gene062651:CDS
ATGATATAGAATGTGCCACC+AGG 0.576586 6.4:-46272561 None:intergenic
TTAACAAATTAACTGGACCA+TGG 0.578865 6.4:+46272817 MS.gene062651:CDS
GGTGGTACTGAAGATAATGA+AGG 0.579997 6.4:+46272849 MS.gene062651:CDS
ATCATAACAAGCACCAGGTG+GGG 0.587156 6.4:-46272955 None:intergenic
TTGGAGGATTCAGCACACTC+GGG 0.592441 6.4:-46272774 None:intergenic
CCAATATCATAACAAGCACC+AGG 0.593116 6.4:-46272960 None:intergenic
TCAATCTTTGGAGCAGCTGG+TGG 0.597041 6.4:+46272588 MS.gene062651:CDS
TGCGATATCAAGTGGTGTGT+TGG 0.602143 6.4:-46272739 None:intergenic
TATCATAACAAGCACCAGGT+GGG 0.615337 6.4:-46272956 None:intergenic
GCTGGTGGTGGATTGAGGCT+TGG 0.621479 6.4:+46272603 MS.gene062651:CDS
AGGCAGACCATTGACAACCT+CGG 0.627216 6.4:-46272673 None:intergenic
AGTTTAGCAGGATAGGTGCA+GGG 0.629579 6.4:+46272700 MS.gene062651:CDS
AAATTAACTGGACCATGGGT+GGG 0.632486 6.4:+46272822 MS.gene062651:CDS
GTGCTTGTTATGATATTGGG+AGG 0.648866 6.4:+46272964 MS.gene062651:CDS
GTACCACCAATACCCACCCA+TGG 0.653889 6.4:-46272834 None:intergenic
GGCATGATGACTTTACTGGA+AGG 0.654511 6.4:+46272985 MS.gene062651:CDS
AGCAGGTGGTTAATGCAAAG+AGG 0.657549 6.4:-46272476 None:intergenic
TGTACTTCAAGATTATTCCG+AGG 0.666999 6.4:+46272656 MS.gene062651:CDS
GTGTGCTGAATCCTCCAAGT+GGG 0.686035 6.4:+46272779 MS.gene062651:CDS
ATATCATAACAAGCACCAGG+TGG 0.697776 6.4:-46272957 None:intergenic
AAAAGCTAATGAAAAGCAGG+TGG 0.699196 6.4:-46272490 None:intergenic
AGTGTGCTGAATCCTCCAAG+TGG 0.720736 6.4:+46272778 MS.gene062651:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTAAAATTCAAAAACA+TGG + chr6.4:46272891-46272910 MS.gene062651:CDS 15.0%
!!! GATGATTTTAACAAATTAAC+TGG + chr6.4:46272810-46272829 MS.gene062651:CDS 20.0%
! ATCAACAAATACAACTTCAT+AGG - chr6.4:46273036-46273055 None:intergenic 25.0%
! TCAACAAATACAACTTCATA+GGG - chr6.4:46273035-46273054 None:intergenic 25.0%
GAACAAGTTATTGACACAAA+TGG + chr6.4:46272525-46272544 MS.gene062651:CDS 30.0%
GAAGGTAAGCATATTATAGA+TGG + chr6.4:46272867-46272886 MS.gene062651:CDS 30.0%
TAACAAATTAACTGGACCAT+GGG + chr6.4:46272818-46272837 MS.gene062651:CDS 30.0%
TAATCTTGAAGTACAGTAAG+TGG - chr6.4:46272651-46272670 None:intergenic 30.0%
TATATCATGCCATCAATCTT+TGG + chr6.4:46272576-46272595 MS.gene062651:CDS 30.0%
TTAACAAATTAACTGGACCA+TGG + chr6.4:46272817-46272836 MS.gene062651:CDS 30.0%
! TAAAGATCTTCCATTTTGAG+AGG - chr6.4:46273060-46273079 None:intergenic 30.0%
AAAAGCTAATGAAAAGCAGG+TGG - chr6.4:46272493-46272512 None:intergenic 35.0%
AAAGAAAGGGTAAGGAATGT+AGG - chr6.4:46272453-46272472 None:intergenic 35.0%
CTTGTAAATGCGATATCAAG+TGG - chr6.4:46272750-46272769 None:intergenic 35.0%
TGCAAAAGCTAATGAAAAGC+AGG - chr6.4:46272496-46272515 None:intergenic 35.0%
TGTACTTCAAGATTATTCCG+AGG + chr6.4:46272656-46272675 MS.gene062651:CDS 35.0%
TTTGTTGATGCCTCTCAAAA+TGG + chr6.4:46273047-46273066 MS.gene062651:CDS 35.0%
!! AATGGAAACCCTATTTTTCC+TGG + chr6.4:46272543-46272562 MS.gene062651:CDS 35.0%
!! CTGGTGCTTGTTATGATATT+GGG + chr6.4:46272961-46272980 MS.gene062651:CDS 35.0%
AAATTAACTGGACCATGGGT+GGG + chr6.4:46272822-46272841 MS.gene062651:CDS 40.0%
AATGTGCCACCAGGAAAAAT+AGG - chr6.4:46272555-46272574 None:intergenic 40.0%
ATATCATAACAAGCACCAGG+TGG - chr6.4:46272960-46272979 None:intergenic 40.0%
ATGATATAGAATGTGCCACC+AGG - chr6.4:46272564-46272583 None:intergenic 40.0%
ATGCAAAGAGGAGCAAAGAA+AGG - chr6.4:46272467-46272486 None:intergenic 40.0%
ATGTGCCACCAGGAAAAATA+GGG - chr6.4:46272554-46272573 None:intergenic 40.0%
CATAGGGATCATTATTAGCC+AGG - chr6.4:46273019-46273038 None:intergenic 40.0%
CCAATATCATAACAAGCACC+AGG - chr6.4:46272963-46272982 None:intergenic 40.0%
CCTATCCTGCTAAACTTTAC+AGG - chr6.4:46272696-46272715 None:intergenic 40.0%
CCTGTAAAGTTTAGCAGGAT+AGG + chr6.4:46272693-46272712 MS.gene062651:CDS 40.0%
GATTATTCCGAGGTTGTCAA+TGG + chr6.4:46272666-46272685 MS.gene062651:CDS 40.0%
TATCATAACAAGCACCAGGT+GGG - chr6.4:46272959-46272978 None:intergenic 40.0%
TGCAAAGAGGAGCAAAGAAA+GGG - chr6.4:46272466-46272485 None:intergenic 40.0%
TGGACAGAACACAAACTTGT+AGG - chr6.4:46272925-46272944 None:intergenic 40.0%
! GGTGGTACTGAAGATAATGA+AGG + chr6.4:46272849-46272868 MS.gene062651:CDS 40.0%
!! CCTGGTGCTTGTTATGATAT+TGG + chr6.4:46272960-46272979 MS.gene062651:CDS 40.0%
!! GTGCTTGTTATGATATTGGG+AGG + chr6.4:46272964-46272983 MS.gene062651:CDS 40.0%
AAGTTTAGCAGGATAGGTGC+AGG + chr6.4:46272699-46272718 MS.gene062651:CDS 45.0%
AGAGGAGCAAAGAAAGGGTA+AGG - chr6.4:46272461-46272480 None:intergenic 45.0%
AGCAGGTGGTTAATGCAAAG+AGG - chr6.4:46272479-46272498 None:intergenic 45.0%
AGTTTAGCAGGATAGGTGCA+GGG + chr6.4:46272700-46272719 MS.gene062651:CDS 45.0%
ATCATAACAAGCACCAGGTG+GGG - chr6.4:46272958-46272977 None:intergenic 45.0%
ATCATCAACCAACACCCACT+TGG - chr6.4:46272796-46272815 None:intergenic 45.0%
CAAATTAACTGGACCATGGG+TGG + chr6.4:46272821-46272840 MS.gene062651:CDS 45.0%
GGCATGATGACTTTACTGGA+AGG + chr6.4:46272985-46273004 MS.gene062651:CDS 45.0%
GTCTGCCTGTAAAGTTTAGC+AGG + chr6.4:46272688-46272707 MS.gene062651:CDS 45.0%
TGACTTTACTGGAAGGCTTC+TGG + chr6.4:46272992-46273011 MS.gene062651:CDS 45.0%
TGCGATATCAAGTGGTGTGT+TGG - chr6.4:46272742-46272761 None:intergenic 45.0%
!! GGAAACCCTATTTTTCCTGG+TGG + chr6.4:46272546-46272565 MS.gene062651:CDS 45.0%
AGGCAGACCATTGACAACCT+CGG - chr6.4:46272676-46272695 None:intergenic 50.0%
AGTGTGCTGAATCCTCCAAG+TGG + chr6.4:46272778-46272797 MS.gene062651:CDS 50.0%
ATCAACCAACACCCACTTGG+AGG - chr6.4:46272793-46272812 None:intergenic 50.0%
CCAGCTGCTCCAAAGATTGA+TGG - chr6.4:46272588-46272607 None:intergenic 50.0%
CTTGGAGGATTCAGCACACT+CGG - chr6.4:46272778-46272797 None:intergenic 50.0%
GTGTGCTGAATCCTCCAAGT+GGG + chr6.4:46272779-46272798 MS.gene062651:CDS 50.0%
TGAATCCTCCAAGTGGGTGT+TGG + chr6.4:46272785-46272804 MS.gene062651:CDS 50.0%
TTGGAGGATTCAGCACACTC+GGG - chr6.4:46272777-46272796 None:intergenic 50.0%
! GGGAGGCATGATGACTTTAC+TGG + chr6.4:46272981-46273000 MS.gene062651:CDS 50.0%
!! CCATCAATCTTTGGAGCAGC+TGG + chr6.4:46272585-46272604 MS.gene062651:CDS 50.0%
!! TCAATCTTTGGAGCAGCTGG+TGG + chr6.4:46272588-46272607 MS.gene062651:CDS 50.0%
ACTGGACCATGGGTGGGTAT+TGG + chr6.4:46272828-46272847 MS.gene062651:CDS 55.0%
GTACCACCAATACCCACCCA+TGG - chr6.4:46272837-46272856 None:intergenic 55.0%
TACTGGAAGGCTTCTGGTCC+TGG + chr6.4:46272998-46273017 MS.gene062651:CDS 55.0%
!! ATCTTTGGAGCAGCTGGTGG+TGG + chr6.4:46272591-46272610 MS.gene062651:CDS 55.0%
CCAGGTGGGGCAGTGATAGA+TGG - chr6.4:46272945-46272964 None:intergenic 60.0%
CCATCTATCACTGCCCCACC+TGG + chr6.4:46272942-46272961 MS.gene062651:CDS 60.0%
!! GAGCAGCTGGTGGTGGATTG+AGG + chr6.4:46272598-46272617 MS.gene062651:CDS 60.0%
!! GCTGGTGGTGGATTGAGGCT+TGG + chr6.4:46272603-46272622 MS.gene062651:CDS 60.0%
!! GGACCATGGGTGGGTATTGG+TGG + chr6.4:46272831-46272850 MS.gene062651:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 46272444 46273085 46272444 ID=MS.gene062651
chr6.4 mRNA 46272444 46273085 46272444 ID=MS.gene062651.t1;Parent=MS.gene062651
chr6.4 exon 46272444 46273085 46272444 ID=MS.gene062651.t1.exon1;Parent=MS.gene062651.t1
chr6.4 CDS 46272444 46273085 46272444 ID=cds.MS.gene062651.t1;Parent=MS.gene062651.t1
Gene Sequence

>MS.gene062651

ATGAAACCTACATTCCTTACCCTTTCTTTGCTCCTCTTTGCATTAACCACCTGCTTTTCATTAGCTTTTGCACAAGCTTCTGAACAAGTTATTGACACAAATGGAAACCCTATTTTTCCTGGTGGCACATTCTATATCATGCCATCAATCTTTGGAGCAGCTGGTGGTGGATTGAGGCTTGGTAAGACAAAAAACTCAAAATGTCCACTTACTGTACTTCAAGATTATTCCGAGGTTGTCAATGGTCTGCCTGTAAAGTTTAGCAGGATAGGTGCAGGGATTGATATAATCTCTACCAACACACCACTTGATATCGCATTTACAAGAAAGCCCGAGTGTGCTGAATCCTCCAAGTGGGTGTTGGTTGATGATTTTAACAAATTAACTGGACCATGGGTGGGTATTGGTGGTACTGAAGATAATGAAGGTAAGCATATTATAGATGGTTTCTTTAAAATTCAAAAACATGGTTTAACTACCTACAAGTTTGTGTTCTGTCCATCTATCACTGCCCCACCTGGTGCTTGTTATGATATTGGGAGGCATGATGACTTTACTGGAAGGCTTCTGGTCCTGGCTAATAATGATCCCTATGAAGTTGTATTTGTTGATGCCTCTCAAAATGGAAGATCTTTAGTTTGA

Protein sequence

>MS.gene062651.t1

MKPTFLTLSLLLFALTTCFSLAFAQASEQVIDTNGNPIFPGGTFYIMPSIFGAAGGGLRLGKTKNSKCPLTVLQDYSEVVNGLPVKFSRIGAGIDIISTNTPLDIAFTRKPECAESSKWVLVDDFNKLTGPWVGIGGTEDNEGKHIIDGFFKIQKHGLTTYKFVFCPSITAPPGACYDIGRHDDFTGRLLVLANNDPYEVVFVDASQNGRSLV