AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063266

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063266.t1 MTR_8g096620 98.006 351 7 0 1 351 1 351 0.0 705
MS.gene063266.t1 MTR_8g096580 47.756 312 149 2 1 311 1 299 5.05e-101 301
MS.gene063266.t1 MTR_5g008850 44.937 316 163 5 1 310 1 311 5.72e-85 261
MS.gene063266.t1 MTR_8g096610 35.233 193 91 7 126 311 54 219 4.15e-26 104
MS.gene063266.t1 MTR_7g080410 33.778 225 139 7 43 260 36 257 1.22e-24 102
MS.gene063266.t1 MTR_7g080410 33.778 225 139 7 43 260 36 257 1.56e-24 101
MS.gene063266.t1 MTR_3g087850 32.447 188 117 6 49 233 30 210 5.08e-19 86.7
MS.gene063266.t1 MTR_7g105590 29.101 189 122 5 47 229 10 192 6.60e-18 81.3
MS.gene063266.t1 MTR_3g085740 27.232 224 147 7 43 263 73 283 2.50e-17 82.0
MS.gene063266.t1 MTR_4g132850 27.111 225 146 4 41 252 3 222 6.58e-17 79.7
MS.gene063266.t1 MTR_8g442750 30.942 223 138 6 40 250 9 227 1.60e-16 79.3
MS.gene063266.t1 MTR_2g022600 30.180 222 132 7 45 250 33 247 2.24e-16 79.7
MS.gene063266.t1 MTR_3g087860 28.390 236 149 9 63 289 30 254 2.43e-16 78.6
MS.gene063266.t1 MTR_1g082760 27.778 198 134 4 40 229 34 230 2.96e-16 78.6
MS.gene063266.t1 MTR_7g113650 26.400 250 171 6 40 277 34 282 1.05e-15 77.0
MS.gene063266.t1 MTR_4g058840 28.358 201 135 6 43 235 9 208 5.28e-15 74.7
MS.gene063266.t1 MTR_4g058820 28.358 201 135 6 43 235 9 208 5.28e-15 74.7
MS.gene063266.t1 MTR_6g023610 28.426 197 129 6 45 234 13 204 9.73e-15 73.9
MS.gene063266.t1 MTR_4g052340 26.047 215 143 6 47 253 16 222 9.97e-15 73.9
MS.gene063266.t1 MTR_4g058810 28.000 200 135 6 44 235 10 208 1.89e-14 73.2
MS.gene063266.t1 MTR_1196s0010 31.674 221 136 6 33 248 262 472 3.47e-14 73.9
MS.gene063266.t1 MTR_6g023590 28.426 197 129 6 45 234 13 204 5.49e-14 71.6
MS.gene063266.t1 MTR_4g052300 25.822 213 146 5 47 253 10 216 5.81e-14 71.6
MS.gene063266.t1 MTR_1g051165 27.000 200 134 5 48 235 17 216 7.48e-14 71.2
MS.gene063266.t1 MTR_4g052350 26.512 215 142 7 47 253 16 222 1.01e-13 70.9
MS.gene063266.t1 MTR_4g052320 27.692 195 129 5 47 235 10 198 1.24e-13 70.5
MS.gene063266.t1 MTR_7g063390 28.879 232 145 9 44 267 15 234 2.09e-13 69.7
MS.gene063266.t1 MTR_4g097510 26.804 194 134 6 48 238 5 193 9.79e-13 68.2
MS.gene063266.t1 MTR_4g086400 26.000 200 136 4 46 233 5 204 1.28e-12 69.3
MS.gene063266.t1 MTR_7g076660 32.215 149 89 3 48 184 8 156 2.44e-12 67.0
MS.gene063266.t1 MTR_7g076660 32.414 145 86 3 52 184 11 155 2.51e-12 67.0
MS.gene063266.t1 MTR_3g086210 28.395 243 151 12 9 233 8 245 3.71e-12 67.0
MS.gene063266.t1 MTR_7g071680 27.933 179 121 3 42 216 26 200 7.51e-12 65.9
MS.gene063266.t1 MTR_4g131740 26.425 193 129 6 47 229 42 231 9.41e-12 65.5
MS.gene063266.t1 MTR_7g076655 31.724 145 87 3 52 184 11 155 1.52e-11 64.7
MS.gene063266.t1 MTR_7g063360 28.634 227 152 7 44 267 15 234 1.88e-11 64.3
MS.gene063266.t1 MTR_4g078630 27.411 197 131 5 42 233 16 205 2.41e-11 63.9
MS.gene063266.t1 MTR_7g094520 27.604 192 133 4 48 233 14 205 2.54e-11 63.5
MS.gene063266.t1 MTR_7g063270 28.125 224 150 7 47 267 18 233 3.20e-11 63.5
MS.gene063266.t1 MTR_3g068070 26.222 225 150 7 47 267 17 229 3.79e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063266.t1 AT5G50600 61.254 351 134 2 1 351 1 349 2.57e-152 432
MS.gene063266.t1 AT5G50700 61.254 351 134 2 1 351 1 349 2.57e-152 432
MS.gene063266.t1 AT5G50770 49.558 339 169 2 1 339 1 337 1.68e-110 326
MS.gene063266.t1 AT3G47350 46.645 313 153 3 1 313 1 299 1.61e-99 296
MS.gene063266.t1 AT5G50690 49.175 303 143 2 1 303 1 292 3.54e-97 290
MS.gene063266.t1 AT5G50590 49.175 303 143 2 1 303 1 292 3.54e-97 290
MS.gene063266.t1 AT3G47360 46.326 313 155 2 1 313 1 300 1.45e-94 284
MS.gene063266.t1 AT4G10020 45.912 318 165 4 1 316 2 314 1.63e-91 279
MS.gene063266.t1 AT3G47350 49.117 283 128 4 1 283 1 267 6.71e-89 270
MS.gene063266.t1 AT5G50690 53.556 239 111 0 1 239 1 239 2.96e-83 253
MS.gene063266.t1 AT5G50590 53.556 239 111 0 1 239 1 239 2.96e-83 253
MS.gene063266.t1 AT3G47350 38.658 313 134 4 1 313 1 255 1.76e-70 221
MS.gene063266.t1 AT3G03330 35.088 228 135 7 17 231 6 233 1.11e-24 102
MS.gene063266.t1 AT5G65205 34.211 190 111 7 49 233 10 190 2.45e-22 95.5
MS.gene063266.t1 AT5G10050 32.308 195 118 6 49 238 9 194 3.69e-22 95.1
MS.gene063266.t1 AT1G24360 29.592 196 131 6 43 234 72 264 8.71e-18 83.2
MS.gene063266.t1 AT1G63380 28.934 197 132 5 48 236 25 221 1.31e-17 82.4
MS.gene063266.t1 AT1G63380 28.934 197 132 5 48 236 18 214 1.89e-17 81.6
MS.gene063266.t1 AT1G63380 28.934 197 132 5 48 236 22 218 2.52e-17 81.6
MS.gene063266.t1 AT1G63380 28.934 197 132 5 48 236 20 216 2.60e-17 81.3
MS.gene063266.t1 AT1G54870 29.648 199 129 6 40 229 76 272 3.55e-17 81.6
MS.gene063266.t1 AT1G54870 29.648 199 129 6 40 229 78 274 3.66e-17 81.6
MS.gene063266.t1 AT1G62610 28.934 197 132 5 48 236 17 213 2.15e-16 78.6
MS.gene063266.t1 AT1G62610 28.934 197 132 5 48 236 18 214 2.18e-16 78.6
MS.gene063266.t1 AT1G62610 28.934 197 132 5 48 236 16 212 2.39e-16 78.6
MS.gene063266.t1 AT1G62610 28.934 197 132 5 48 236 22 218 2.71e-16 78.6
MS.gene063266.t1 AT3G05260 29.146 199 127 7 39 227 30 224 2.74e-16 78.6
MS.gene063266.t1 AT3G12800 30.137 219 130 6 40 243 5 215 7.16e-16 77.4
MS.gene063266.t1 AT3G55310 28.000 200 127 6 48 235 20 214 3.75e-15 75.1
MS.gene063266.t1 AT2G17845 27.500 200 133 6 48 236 50 248 1.53e-14 73.9
MS.gene063266.t1 AT4G05530 26.804 194 130 4 41 229 5 191 2.98e-14 72.0
MS.gene063266.t1 AT3G55290 28.061 196 134 5 48 236 20 215 4.54e-14 72.0
MS.gene063266.t1 AT3G55290 28.061 196 134 5 48 236 21 216 4.55e-14 72.0
MS.gene063266.t1 AT5G06060 28.261 230 145 9 44 267 8 223 5.63e-14 71.6
MS.gene063266.t1 AT3G06060 31.156 199 119 7 52 238 44 236 1.42e-13 71.2
MS.gene063266.t1 AT3G06060 31.156 199 119 7 52 238 61 253 1.81e-13 70.9
MS.gene063266.t1 AT3G46170 27.041 196 136 5 48 236 29 224 1.88e-13 70.5
MS.gene063266.t1 AT4G24050 28.638 213 122 7 52 237 39 248 1.90e-12 67.4
MS.gene063266.t1 AT4G13180 25.764 229 149 6 45 255 13 238 1.98e-12 67.0
MS.gene063266.t1 AT2G29290 30.493 223 133 9 52 267 14 221 2.76e-12 66.6
MS.gene063266.t1 AT1G67730 25.468 267 175 12 16 261 12 275 3.07e-12 67.0
MS.gene063266.t1 AT4G24050 28.638 213 122 7 52 237 39 248 3.18e-12 67.0
MS.gene063266.t1 AT5G18210 26.180 233 148 7 44 255 7 236 5.70e-12 65.9
MS.gene063266.t1 AT5G18210 26.180 233 148 7 44 255 7 236 6.05e-12 65.9
MS.gene063266.t1 AT2G29370 28.384 229 146 7 44 267 15 230 1.68e-11 64.3
MS.gene063266.t1 AT5G19200 29.949 197 121 6 50 235 40 230 1.88e-11 64.7
MS.gene063266.t1 AT1G07440 27.679 224 145 7 48 267 15 225 3.48e-11 63.5
MS.gene063266.t1 AT3G03980 26.829 205 137 5 42 233 11 215 4.70e-11 63.2
MS.gene063266.t1 AT3G26760 25.980 204 143 5 35 234 26 225 5.00e-11 63.2

Find 77 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTATATATACTCCTTC+TGG 0.214734 4.2:-7069886 None:intergenic
TTGCTAGGTGCTTCTCTTGC+TGG 0.240546 4.2:-7070493 None:intergenic
TCCTTTCATCAATTGATTCT+TGG 0.265350 4.2:+7069853 MS.gene063266:CDS
GATGAAACCGTTAACCATTT+TGG 0.281093 4.2:+7069480 MS.gene063266:CDS
GCTGATAGAGCTCGTGATTT+TGG 0.286452 4.2:+7069393 MS.gene063266:CDS
AAACCCCACTCCAACAATTC+TGG 0.298326 4.2:-7070436 None:intergenic
TTTCTAAAGTTGATGATTGT+AGG 0.326151 4.2:+7069448 MS.gene063266:CDS
TTTGGTAGGACACAAACTTT+TGG 0.338368 4.2:+7069766 MS.gene063266:intron
AGGGAAGAATGTAAAGATAA+TGG 0.356930 4.2:-7069156 None:intergenic
CATACATACGTCTACCAAAA+TGG 0.358019 4.2:-7069494 None:intergenic
AAGAATTTGAAGGTCCAATA+TGG 0.365021 4.2:-7069128 None:intergenic
ATTGCCACCAGAAGGCAAAA+TGG 0.369505 4.2:+7070150 MS.gene063266:CDS
TTGTTACATCTCTCATATCT+TGG 0.383129 4.2:-7070180 None:intergenic
TTCTTCCCTGAAAATGTTGC+TGG 0.384509 4.2:+7069170 MS.gene063266:CDS
ATGCATTACAGTGGATCATT+TGG 0.398219 4.2:+7069591 MS.gene063266:intron
GGTTCAGGAACTTATGAATA+AGG 0.407519 4.2:-7069058 None:intergenic
CACATCACTGGTGCTTCCTC+TGG 0.411745 4.2:+7069203 MS.gene063266:CDS
ACTGGTGCTTCCTCTGGTAT+TGG 0.420526 4.2:+7069209 MS.gene063266:CDS
TGGCAATAAGACTTTGCCTT+TGG 0.423226 4.2:-7070134 None:intergenic
AGTTGATGATTGTAGGCGTT+TGG 0.425267 4.2:+7069455 MS.gene063266:CDS
ACATTATATATACTCCTTCT+GGG 0.435152 4.2:-7069885 None:intergenic
ACGTCTACCAAAATGGTTAA+CGG 0.443115 4.2:-7069487 None:intergenic
TCCAATATGGTGGTAAGAAA+AGG 0.443917 4.2:-7069115 None:intergenic
GAAAAGGAGTAAGGAAAAGA+AGG 0.450873 4.2:-7069099 None:intergenic
AGTAAGGAAAAGAAGGTGAA+TGG 0.457421 4.2:-7069092 None:intergenic
ATGGAGGAGCTACTAGGTTC+AGG 0.479762 4.2:-7069073 None:intergenic
TGAGGTGTGGTAAAGCAAAA+CGG 0.485877 4.2:-7069806 None:intergenic
GTGTCCAGAATTGTTGGAGT+GGG 0.486451 4.2:+7070432 MS.gene063266:CDS
TTGCCACCAGAAGGCAAAAT+GGG 0.488945 4.2:+7070151 MS.gene063266:CDS
TATATACTCCTTCTGGGTGC+AGG 0.491452 4.2:-7069879 None:intergenic
GAGCTCTATCAGCAACTTCA+CGG 0.495626 4.2:-7069383 None:intergenic
AAGTCTTTATGAAACATTGA+GGG 0.498519 4.2:+7070048 MS.gene063266:CDS
TGGTGGTAAGAAAAGGAGTA+AGG 0.505040 4.2:-7069108 None:intergenic
CCTCTGCTGTTTCTGAGGTG+TGG 0.510515 4.2:-7069819 None:intergenic
GCACGCATTATAATAACATC+AGG 0.515853 4.2:-7069420 None:intergenic
TAGCTTTAAATTACCATGAG+AGG 0.516356 4.2:-7069679 None:intergenic
GAAACATTGAGGGTTGAAGT+TGG 0.516747 4.2:+7070058 MS.gene063266:CDS
ATGGTACATTGAGGGGAGAT+AGG 0.520717 4.2:+7070359 MS.gene063266:CDS
GAGGTGTGGTAAAGCAAAAC+GGG 0.520958 4.2:-7069805 None:intergenic
TACCATGAGAGGTCTCCAAT+TGG 0.521548 4.2:-7069668 None:intergenic
AGTGTTGTGTCCAGAATTGT+TGG 0.521927 4.2:+7070426 MS.gene063266:CDS
AGGTGAATGGAGGAGCTACT+AGG 0.526268 4.2:-7069079 None:intergenic
AATGGAAAGGAAGAATTTGA+AGG 0.527999 4.2:-7069138 None:intergenic
AATAGTGAATGGTACATTGA+GGG 0.532567 4.2:+7070351 MS.gene063266:CDS
ACCAGAAGGCAAAATGGGCG+TGG 0.534597 4.2:+7070156 MS.gene063266:CDS
TCAGGAGATCGAATTGAAGA+TGG 0.534824 4.2:-7070556 None:intergenic
ACTCATTCAGTCTTGACCTC+AGG 0.535309 4.2:-7070574 None:intergenic
ATGTAACTGATATCACCAAT+TGG 0.537484 4.2:+7069653 MS.gene063266:CDS
GGTAAAGTTGTTCACATCAC+TGG 0.554581 4.2:+7069191 MS.gene063266:CDS
AATTCAGATTCTATGTAACC+TGG 0.554617 4.2:-7070109 None:intergenic
TAAGTCTTTATGAAACATTG+AGG 0.565380 4.2:+7070047 MS.gene063266:CDS
CACCAATTGGAGACCTCTCA+TGG 0.567087 4.2:+7069666 MS.gene063266:CDS
TCCAAGAATCAATTGATGAA+AGG 0.573578 4.2:-7069854 None:intergenic
CAATAGTGAATGGTACATTG+AGG 0.574939 4.2:+7070350 MS.gene063266:CDS
CATTCACTCACTGATCCCAC+TGG 0.576814 4.2:-7070319 None:intergenic
AGTTACATGTTCAAACATCA+TGG 0.578048 4.2:-7069638 None:intergenic
CTTGGATGCCTGCACCCAGA+AGG 0.586038 4.2:+7069871 MS.gene063266:CDS
TATGTCATTCTAAACCATGC+TGG 0.590361 4.2:-7070394 None:intergenic
TGTGCAAAATCAATAGTGAA+TGG 0.591847 4.2:+7070340 MS.gene063266:CDS
TGCTTCCTCTGGTATTGGAG+AGG 0.597134 4.2:+7069214 MS.gene063266:CDS
TGAAGTGAGTGCAACTCCAG+TGG 0.598795 4.2:+7070303 MS.gene063266:CDS
GGTATTTGACTGCACCAGCA+TGG 0.600958 4.2:+7070380 MS.gene063266:CDS
TGTCCAGAATTGTTGGAGTG+GGG 0.604050 4.2:+7070433 MS.gene063266:CDS
AAGGAAAAGAAGGTGAATGG+AGG 0.605287 4.2:-7069089 None:intergenic
AAAGTCTTATTGCCACCAGA+AGG 0.611122 4.2:+7070142 MS.gene063266:CDS
GAAGTGAGTGCAACTCCAGT+GGG 0.616438 4.2:+7070304 MS.gene063266:CDS
ATCTTCAATTCGATCTCCTG+AGG 0.626026 4.2:+7070558 MS.gene063266:CDS
TGTGTCCAGAATTGTTGGAG+TGG 0.626150 4.2:+7070431 MS.gene063266:CDS
AAACAGCAGAGGCAAAATTG+TGG 0.631748 4.2:+7069830 MS.gene063266:CDS
ATAGAATCTGAATTAACCAA+AGG 0.631775 4.2:+7070118 MS.gene063266:CDS
ATAGTGAATGGTACATTGAG+GGG 0.634116 4.2:+7070352 MS.gene063266:CDS
GGTGTAACAATTGTTACACC+AGG 0.641548 4.2:+7070091 MS.gene063266:CDS
AGAATGTAAAGATAATGGAA+AGG 0.645582 4.2:-7069151 None:intergenic
GCTTACGAGTATGCTAAGAG+AGG 0.664397 4.2:+7069321 MS.gene063266:CDS
AATTTGAAGGTCCAATATGG+TGG 0.665878 4.2:-7069125 None:intergenic
GTTGAAGTTGGAGCAGACGT+AGG 0.674435 4.2:+7070070 MS.gene063266:CDS
CCACACCTCAGAAACAGCAG+AGG 0.715008 4.2:+7069819 MS.gene063266:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAATTAATATTTGTTTA+AGG - chr4.2:7069535-7069554 None:intergenic 10.0%
!!! ATTTTAATTTTTGTAGCATT+TGG + chr4.2:7069299-7069318 MS.gene063266:intron 15.0%
!! TGTTATGATGATATATATGT+AGG + chr4.2:7070279-7070298 MS.gene063266:intron 20.0%
!!! TAATTATTTGCTGTGTATTT+TGG + chr4.2:7069748-7069767 MS.gene063266:intron 20.0%
!!! TTTAATTTACCTAGTAATAC+AGG + chr4.2:7070220-7070239 MS.gene063266:intron 20.0%
! AAGTCTTTATGAAACATTGA+GGG + chr4.2:7070048-7070067 MS.gene063266:CDS 25.0%
! ACATTATATATACTCCTTCT+GGG - chr4.2:7069888-7069907 None:intergenic 25.0%
! ATAGAATCTGAATTAACCAA+AGG + chr4.2:7070118-7070137 MS.gene063266:CDS 25.0%
! TAAGTCTTTATGAAACATTG+AGG + chr4.2:7070047-7070066 MS.gene063266:CDS 25.0%
! TACATTATATATACTCCTTC+TGG - chr4.2:7069889-7069908 None:intergenic 25.0%
!!! AGAATGTAAAGATAATGGAA+AGG - chr4.2:7069154-7069173 None:intergenic 25.0%
!!! TTTCTAAAGTTGATGATTGT+AGG + chr4.2:7069448-7069467 MS.gene063266:CDS 25.0%
AAGAATTTGAAGGTCCAATA+TGG - chr4.2:7069131-7069150 None:intergenic 30.0%
AATAGTGAATGGTACATTGA+GGG + chr4.2:7070351-7070370 MS.gene063266:CDS 30.0%
AATGGAAAGGAAGAATTTGA+AGG - chr4.2:7069141-7069160 None:intergenic 30.0%
AATTCAGATTCTATGTAACC+TGG - chr4.2:7070112-7070131 None:intergenic 30.0%
AGGGAAGAATGTAAAGATAA+TGG - chr4.2:7069159-7069178 None:intergenic 30.0%
AGTTACATGTTCAAACATCA+TGG - chr4.2:7069641-7069660 None:intergenic 30.0%
ATGTAACTGATATCACCAAT+TGG + chr4.2:7069653-7069672 MS.gene063266:CDS 30.0%
ATTCCCAATTCAACTCAATA+AGG - chr4.2:7069724-7069743 None:intergenic 30.0%
TAGTTATAGCCTGTATTACT+AGG - chr4.2:7070232-7070251 None:intergenic 30.0%
TCCAAGAATCAATTGATGAA+AGG - chr4.2:7069857-7069876 None:intergenic 30.0%
TGTGCAAAATCAATAGTGAA+TGG + chr4.2:7070340-7070359 MS.gene063266:CDS 30.0%
TTGTTACATCTCTCATATCT+TGG - chr4.2:7070183-7070202 None:intergenic 30.0%
! AAAAAGATTTTGGATGCTAC+TGG + chr4.2:7070514-7070533 MS.gene063266:CDS 30.0%
! TCCTTTCATCAATTGATTCT+TGG + chr4.2:7069853-7069872 MS.gene063266:CDS 30.0%
!! TAGCTTTAAATTACCATGAG+AGG - chr4.2:7069682-7069701 None:intergenic 30.0%
!! TATTGAGTTGAATTGGGAAT+TGG + chr4.2:7069724-7069743 MS.gene063266:intron 30.0%
!!! TATTTGCTGTGTATTTTGGT+AGG + chr4.2:7069752-7069771 MS.gene063266:intron 30.0%
AATTTGAAGGTCCAATATGG+TGG - chr4.2:7069128-7069147 None:intergenic 35.0%
ACGTCTACCAAAATGGTTAA+CGG - chr4.2:7069490-7069509 None:intergenic 35.0%
AGTAAGGAAAAGAAGGTGAA+TGG - chr4.2:7069095-7069114 None:intergenic 35.0%
ATAGTGAATGGTACATTGAG+GGG + chr4.2:7070352-7070371 MS.gene063266:CDS 35.0%
ATGCATTACAGTGGATCATT+TGG + chr4.2:7069591-7069610 MS.gene063266:intron 35.0%
CAATAGTGAATGGTACATTG+AGG + chr4.2:7070350-7070369 MS.gene063266:CDS 35.0%
CATACATACGTCTACCAAAA+TGG - chr4.2:7069497-7069516 None:intergenic 35.0%
GAAAAGGAGTAAGGAAAAGA+AGG - chr4.2:7069102-7069121 None:intergenic 35.0%
GCACGCATTATAATAACATC+AGG - chr4.2:7069423-7069442 None:intergenic 35.0%
GCTAACTACATGACTTTATC+TGG + chr4.2:7069698-7069717 MS.gene063266:intron 35.0%
GGTTCAGGAACTTATGAATA+AGG - chr4.2:7069061-7069080 None:intergenic 35.0%
TATGTCATTCTAAACCATGC+TGG - chr4.2:7070397-7070416 None:intergenic 35.0%
TCCAATATGGTGGTAAGAAA+AGG - chr4.2:7069118-7069137 None:intergenic 35.0%
TTCTCTGTAATGCATTACAG+TGG + chr4.2:7069582-7069601 MS.gene063266:intron 35.0%
! ACTTTACCAGCAACATTTTC+AGG - chr4.2:7069179-7069198 None:intergenic 35.0%
! AGCACCTAGCAAAAAGATTT+TGG + chr4.2:7070504-7070523 MS.gene063266:CDS 35.0%
! CTTTACCAGCAACATTTTCA+GGG - chr4.2:7069178-7069197 None:intergenic 35.0%
! GATGAAACCGTTAACCATTT+TGG + chr4.2:7069480-7069499 MS.gene063266:CDS 35.0%
! GCATCCAAAATCTTTTTGCT+AGG - chr4.2:7070511-7070530 None:intergenic 35.0%
! TCCTTTTCTTACCACCATAT+TGG + chr4.2:7069114-7069133 MS.gene063266:CDS 35.0%
! TTGGTAGGACACAAACTTTT+GGG + chr4.2:7069767-7069786 MS.gene063266:intron 35.0%
! TTTGGTAGGACACAAACTTT+TGG + chr4.2:7069766-7069785 MS.gene063266:intron 35.0%
!! TGGCCTTATTGAGTTGAATT+GGG + chr4.2:7069718-7069737 MS.gene063266:intron 35.0%
AAACAGCAGAGGCAAAATTG+TGG + chr4.2:7069830-7069849 MS.gene063266:CDS 40.0%
AAGGAAAAGAAGGTGAATGG+AGG - chr4.2:7069092-7069111 None:intergenic 40.0%
AGTGTTGTGTCCAGAATTGT+TGG + chr4.2:7070426-7070445 MS.gene063266:CDS 40.0%
ATCTTCAATTCGATCTCCTG+AGG + chr4.2:7070558-7070577 MS.gene063266:CDS 40.0%
GAAACATTGAGGGTTGAAGT+TGG + chr4.2:7070058-7070077 MS.gene063266:CDS 40.0%
GGTAAAGTTGTTCACATCAC+TGG + chr4.2:7069191-7069210 MS.gene063266:CDS 40.0%
GGTGTAACAATTGTTACACC+AGG + chr4.2:7070091-7070110 MS.gene063266:CDS 40.0%
TCAGGAGATCGAATTGAAGA+TGG - chr4.2:7070559-7070578 None:intergenic 40.0%
TGAGGTGTGGTAAAGCAAAA+CGG - chr4.2:7069809-7069828 None:intergenic 40.0%
TGGCAATAAGACTTTGCCTT+TGG - chr4.2:7070137-7070156 None:intergenic 40.0%
TGGTGGTAAGAAAAGGAGTA+AGG - chr4.2:7069111-7069130 None:intergenic 40.0%
TTCTTCCCTGAAAATGTTGC+TGG + chr4.2:7069170-7069189 MS.gene063266:CDS 40.0%
! TGGTAGGACACAAACTTTTG+GGG + chr4.2:7069768-7069787 MS.gene063266:intron 40.0%
! TTTTACCTCTCCAATACCAG+AGG - chr4.2:7069222-7069241 None:intergenic 40.0%
!! AAAGTCTTATTGCCACCAGA+AGG + chr4.2:7070142-7070161 MS.gene063266:CDS 40.0%
!! AGTTGATGATTGTAGGCGTT+TGG + chr4.2:7069455-7069474 MS.gene063266:CDS 40.0%
!! CTGGCCTTATTGAGTTGAAT+TGG + chr4.2:7069717-7069736 MS.gene063266:intron 40.0%
AAACCCCACTCCAACAATTC+TGG - chr4.2:7070439-7070458 None:intergenic 45.0%
AGTAAACACTACTACCCTGC+AGG + chr4.2:7070006-7070025 MS.gene063266:intron 45.0%
ATGGTACATTGAGGGGAGAT+AGG + chr4.2:7070359-7070378 MS.gene063266:CDS 45.0%
ATTGCCACCAGAAGGCAAAA+TGG + chr4.2:7070150-7070169 MS.gene063266:CDS 45.0%
GAGCTCTATCAGCAACTTCA+CGG - chr4.2:7069386-7069405 None:intergenic 45.0%
GAGGTGTGGTAAAGCAAAAC+GGG - chr4.2:7069808-7069827 None:intergenic 45.0%
GCTTACGAGTATGCTAAGAG+AGG + chr4.2:7069321-7069340 MS.gene063266:CDS 45.0%
GTGTCCAGAATTGTTGGAGT+GGG + chr4.2:7070432-7070451 MS.gene063266:CDS 45.0%
TACCATGAGAGGTCTCCAAT+TGG - chr4.2:7069671-7069690 None:intergenic 45.0%
TATATACTCCTTCTGGGTGC+AGG - chr4.2:7069882-7069901 None:intergenic 45.0%
TGTCCAGAATTGTTGGAGTG+GGG + chr4.2:7070433-7070452 MS.gene063266:CDS 45.0%
TGTGTCCAGAATTGTTGGAG+TGG + chr4.2:7070431-7070450 MS.gene063266:CDS 45.0%
TTGCCACCAGAAGGCAAAAT+GGG + chr4.2:7070151-7070170 MS.gene063266:CDS 45.0%
! GCTGATAGAGCTCGTGATTT+TGG + chr4.2:7069393-7069412 MS.gene063266:CDS 45.0%
!!! TTTTGCCTCTGCTGTTTCTG+AGG - chr4.2:7069827-7069846 None:intergenic 45.0%
ACTGGTGCTTCCTCTGGTAT+TGG + chr4.2:7069209-7069228 MS.gene063266:CDS 50.0%
AGGTGAATGGAGGAGCTACT+AGG - chr4.2:7069082-7069101 None:intergenic 50.0%
ATGGAGGAGCTACTAGGTTC+AGG - chr4.2:7069076-7069095 None:intergenic 50.0%
CACCAATTGGAGACCTCTCA+TGG + chr4.2:7069666-7069685 MS.gene063266:CDS 50.0%
CATTCACTCACTGATCCCAC+TGG - chr4.2:7070322-7070341 None:intergenic 50.0%
GAAGTGAGTGCAACTCCAGT+GGG + chr4.2:7070304-7070323 MS.gene063266:CDS 50.0%
GGTATTTGACTGCACCAGCA+TGG + chr4.2:7070380-7070399 MS.gene063266:CDS 50.0%
GTTGAAGTTGGAGCAGACGT+AGG + chr4.2:7070070-7070089 MS.gene063266:CDS 50.0%
TGAAGTGAGTGCAACTCCAG+TGG + chr4.2:7070303-7070322 MS.gene063266:CDS 50.0%
TTGCTAGGTGCTTCTCTTGC+TGG - chr4.2:7070496-7070515 None:intergenic 50.0%
! TGCTTCCTCTGGTATTGGAG+AGG + chr4.2:7069214-7069233 MS.gene063266:CDS 50.0%
ACCAGAAGGCAAAATGGGCG+TGG + chr4.2:7070156-7070175 MS.gene063266:CDS 55.0%
CACATCACTGGTGCTTCCTC+TGG + chr4.2:7069203-7069222 MS.gene063266:CDS 55.0%
CCACACCTCAGAAACAGCAG+AGG + chr4.2:7069819-7069838 MS.gene063266:CDS 55.0%
! ACGCCCATTTTGCCTTCTGG+TGG - chr4.2:7070157-7070176 None:intergenic 55.0%
! TCCACGCCCATTTTGCCTTC+TGG - chr4.2:7070160-7070179 None:intergenic 55.0%
!! ATGCTGCTTTGCTTGCCTGC+AGG - chr4.2:7070024-7070043 None:intergenic 55.0%
!! CCTCTGCTGTTTCTGAGGTG+TGG - chr4.2:7069822-7069841 None:intergenic 55.0%
!! TGCTGCTTTGCTTGCCTGCA+GGG - chr4.2:7070023-7070042 None:intergenic 55.0%
CTTGGATGCCTGCACCCAGA+AGG + chr4.2:7069871-7069890 MS.gene063266:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 7069053 7070594 7069053 ID=MS.gene063266
chr4.2 mRNA 7069053 7070594 7069053 ID=MS.gene063266.t1;Parent=MS.gene063266
chr4.2 exon 7069053 7069235 7069053 ID=MS.gene063266.t1.exon1;Parent=MS.gene063266.t1
chr4.2 CDS 7069053 7069235 7069053 ID=cds.MS.gene063266.t1;Parent=MS.gene063266.t1
chr4.2 exon 7069315 7069507 7069315 ID=MS.gene063266.t1.exon2;Parent=MS.gene063266.t1
chr4.2 CDS 7069315 7069507 7069315 ID=cds.MS.gene063266.t1;Parent=MS.gene063266.t1
chr4.2 exon 7069602 7069687 7069602 ID=MS.gene063266.t1.exon3;Parent=MS.gene063266.t1
chr4.2 CDS 7069602 7069687 7069602 ID=cds.MS.gene063266.t1;Parent=MS.gene063266.t1
chr4.2 exon 7069774 7069905 7069774 ID=MS.gene063266.t1.exon4;Parent=MS.gene063266.t1
chr4.2 CDS 7069774 7069905 7069774 ID=cds.MS.gene063266.t1;Parent=MS.gene063266.t1
chr4.2 exon 7070028 7070195 7070028 ID=MS.gene063266.t1.exon5;Parent=MS.gene063266.t1
chr4.2 CDS 7070028 7070195 7070028 ID=cds.MS.gene063266.t1;Parent=MS.gene063266.t1
chr4.2 exon 7070301 7070594 7070301 ID=MS.gene063266.t1.exon6;Parent=MS.gene063266.t1
chr4.2 CDS 7070301 7070594 7070301 ID=cds.MS.gene063266.t1;Parent=MS.gene063266.t1
Gene Sequence

>MS.gene063266

ATGGACCTTATTCATAAGTTCCTGAACCTAGTAGCTCCTCCATTCACCTTCTTTTCCTTACTCCTTTTCTTACCACCATATTGGACCTTCAAATTCTTCCTTTCCATTATCTTTACATTCTTCCCTGAAAATGTTGCTGGTAAAGTTGTTCACATCACTGGTGCTTCCTCTGGTATTGGAGAGGTAAAACATATAGTATTCTCAATTAATTCATATATACACTAACTAACATTATTATATTTAATGATTTTAATTTTTGTAGCATTTGGCTTACGAGTATGCTAAGAGAGGTGCACGCTTAGCACTTTCTGCTAGAAGAGATACAGCACTCCGTGAAGTTGCTGATAGAGCTCGTGATTTTGGTTCTCCTGATGTTATTATAATGCGTGCAGATGTTTCTAAAGTTGATGATTGTAGGCGTTTGGTCGATGAAACCGTTAACCATTTTGGTAGACGTATGTATGTGACAGTCAAAGTTTCCTTAAACAAATATTAATTAATCACATAAATACATATCTTATACTGTTAATTCTCTGTAATGCATTACAGTGGATCATTTGGTAAACAATGCTGCAATCAGTGCTGCCATGATGTTTGAACATGTAACTGATATCACCAATTGGAGACCTCTCATGGTAATTTAAAGCTAACTACATGACTTTATCTGGCCTTATTGAGTTGAATTGGGAATTGGCTAATTATTTGCTGTGTATTTTGGTAGGACACAAACTTTTGGGGATCAGTTTACACAACCCGTTTTGCTTTACCACACCTCAGAAACAGCAGAGGCAAAATTGTGGTCCTTTCATCAATTGATTCTTGGATGCCTGCACCCAGAAGGAGTATATATAATGTACTAAGTGACTGATATATTACTATTATAATCTATCTCTATTTTCAATTAATATTGTTCAATATATTATATTCTTCATATTATTTCATTTAATTATTAAAGTAAACACTACTACCCTGCAGGCAAGCAAAGCAGCATTAGTAAGTCTTTATGAAACATTGAGGGTTGAAGTTGGAGCAGACGTAGGTGTAACAATTGTTACACCAGGTTACATAGAATCTGAATTAACCAAAGGCAAAGTCTTATTGCCACCAGAAGGCAAAATGGGCGTGGACCAAGATATGAGAGATGTAACAAAATTTTCAATCTTATCTTTTAATTTACCTAGTAATACAGGCTATAACTATTCATATACAGTAATTTACATAATTAATGTTATGATGATATATATGTAGGTTGAAGTGAGTGCAACTCCAGTGGGATCAGTGAGTGAATGTGCAAAATCAATAGTGAATGGTACATTGAGGGGAGATAGGTATTTGACTGCACCAGCATGGTTTAGAATGACATATGTTGTGAAAGTGTTGTGTCCAGAATTGTTGGAGTGGGGTTTCAGAATGTTGTACTTGACTAGATCAAGTAATACTCCAGCAAGAGAAGCACCTAGCAAAAAGATTTTGGATGCTACTGGAATCAAAAACGTGTTCTATCCATCTTCAATTCGATCTCCTGAGGTCAAGACTGAATGA
Protein sequence

>MS.gene063266.t1

MDLIHKFLNLVAPPFTFFSLLLFLPPYWTFKFFLSIIFTFFPENVAGKVVHITGASSGIGEHLAYEYAKRGARLALSARRDTALREVADRARDFGSPDVIIMRADVSKVDDCRRLVDETVNHFGRLDHLVNNAAISAAMMFEHVTDITNWRPLMDTNFWGSVYTTRFALPHLRNSRGKIVVLSSIDSWMPAPRRSIYNASKAALVSLYETLRVEVGADVGVTIVTPGYIESELTKGKVLLPPEGKMGVDQDMRDVEVSATPVGSVSECAKSIVNGTLRGDRYLTAPAWFRMTYVVKVLCPELLEWGFRMLYLTRSSNTPAREAPSKKILDATGIKNVFYPSSIRSPEVKTE