Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065201.t1 | XP_013446118.1 | 99.7 | 332 | 1 | 0 | 1 | 332 | 1 | 332 | 6.70E-173 | 616.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065201.t1 | Q38942 | 85.6 | 333 | 47 | 1 | 1 | 332 | 1 | 333 | 3.3e-152 | 539.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065201.t1 | A0A072U2U6 | 99.7 | 332 | 1 | 0 | 1 | 332 | 1 | 332 | 4.8e-173 | 616.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004979 | MS.gene065201 | PPI |
| MS.gene92360 | MS.gene065201 | PPI |
| MS.gene022537 | MS.gene065201 | PPI |
| MS.gene79186 | MS.gene065201 | PPI |
| MS.gene065809 | MS.gene065201 | PPI |
| MS.gene024963 | MS.gene065201 | PPI |
| MS.gene000251 | MS.gene065201 | PPI |
| MS.gene065201 | MS.gene85897 | PPI |
| MS.gene07517 | MS.gene065201 | PPI |
| MS.gene060071 | MS.gene065201 | PPI |
| MS.gene065201 | MS.gene047200 | PPI |
| MS.gene060069 | MS.gene065201 | PPI |
| MS.gene065201 | MS.gene40528 | PPI |
| MS.gene79188 | MS.gene065201 | PPI |
| MS.gene53114 | MS.gene065201 | PPI |
| MS.gene060067 | MS.gene065201 | PPI |
| MS.gene55320 | MS.gene065201 | PPI |
| MS.gene86831 | MS.gene065201 | PPI |
| MS.gene40528 | MS.gene065201 | PPI |
| MS.gene00617 | MS.gene065201 | PPI |
| MS.gene072058 | MS.gene065201 | PPI |
| MS.gene046944 | MS.gene065201 | PPI |
| MS.gene61568 | MS.gene065201 | PPI |
| MS.gene62514 | MS.gene065201 | PPI |
| MS.gene066387 | MS.gene065201 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065201.t1 | MTR_8g068875 | 99.398 | 332 | 2 | 0 | 1 | 332 | 1 | 332 | 0.0 | 701 |
| MS.gene065201.t1 | MTR_1g492860 | 87.952 | 332 | 38 | 1 | 1 | 332 | 1 | 330 | 0.0 | 609 |
| MS.gene065201.t1 | MTR_2g008410 | 85.266 | 319 | 46 | 1 | 15 | 332 | 17 | 335 | 0.0 | 598 |
| MS.gene065201.t1 | MTR_2g008410 | 80.564 | 319 | 43 | 2 | 15 | 332 | 17 | 317 | 0.0 | 553 |
| MS.gene065201.t1 | MTR_2g008410 | 88.476 | 269 | 31 | 0 | 64 | 332 | 2 | 270 | 0.0 | 526 |
| MS.gene065201.t1 | MTR_2g008410 | 82.900 | 269 | 28 | 1 | 64 | 332 | 2 | 252 | 3.15e-173 | 482 |
| MS.gene065201.t1 | MTR_5g005120 | 34.295 | 312 | 186 | 6 | 18 | 318 | 15 | 318 | 4.06e-59 | 194 |
| MS.gene065201.t1 | MTR_5g043000 | 27.164 | 335 | 218 | 8 | 8 | 335 | 2 | 317 | 6.87e-29 | 114 |
| MS.gene065201.t1 | MTR_2g436510 | 24.516 | 310 | 191 | 11 | 13 | 309 | 159 | 438 | 5.97e-13 | 70.1 |
| MS.gene065201.t1 | MTR_6g004040 | 34.211 | 114 | 70 | 1 | 28 | 141 | 105 | 213 | 8.95e-12 | 66.6 |
| MS.gene065201.t1 | MTR_2g012630 | 33.333 | 114 | 71 | 1 | 28 | 141 | 104 | 212 | 1.65e-11 | 66.2 |
| MS.gene065201.t1 | MTR_2g012630 | 33.333 | 114 | 71 | 1 | 28 | 141 | 104 | 212 | 3.17e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065201.t1 | AT1G80670 | 84.384 | 333 | 51 | 1 | 1 | 332 | 1 | 333 | 0.0 | 608 |
| MS.gene065201.t1 | AT1G15850 | 72.143 | 140 | 37 | 1 | 1 | 138 | 1 | 140 | 7.58e-68 | 210 |
| MS.gene065201.t1 | AT3G19590 | 35.714 | 322 | 188 | 7 | 9 | 319 | 2 | 315 | 1.43e-61 | 201 |
| MS.gene065201.t1 | AT1G49910 | 33.962 | 318 | 191 | 7 | 13 | 319 | 5 | 314 | 1.67e-57 | 190 |
| MS.gene065201.t1 | AT1G69400 | 25.382 | 327 | 213 | 8 | 18 | 335 | 7 | 311 | 2.74e-29 | 115 |
| MS.gene065201.t1 | AT1G69400 | 25.197 | 254 | 166 | 7 | 18 | 258 | 7 | 249 | 7.58e-22 | 94.0 |
| MS.gene065201.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 62 | 170 | 2.35e-13 | 71.6 |
| MS.gene065201.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 2.36e-13 | 71.6 |
| MS.gene065201.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 1.76e-12 | 69.3 |
| MS.gene065201.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 2.45e-13 | 71.6 |
| MS.gene065201.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 1.59e-12 | 69.3 |
| MS.gene065201.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 2.49e-13 | 71.6 |
| MS.gene065201.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 1.68e-12 | 69.3 |
| MS.gene065201.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 4.54e-13 | 70.9 |
| MS.gene065201.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 6.66e-13 | 70.5 |
| MS.gene065201.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 6.85e-13 | 70.5 |
| MS.gene065201.t1 | AT4G15900 | 24.194 | 310 | 192 | 11 | 13 | 309 | 165 | 444 | 9.85e-13 | 69.3 |
| MS.gene065201.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 4.65e-12 | 67.8 |
| MS.gene065201.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 4.65e-12 | 67.8 |
| MS.gene065201.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 4.74e-12 | 67.8 |
| MS.gene065201.t1 | AT5G67320 | 21.656 | 314 | 213 | 7 | 13 | 303 | 254 | 557 | 6.65e-12 | 67.0 |
| MS.gene065201.t1 | AT5G08390 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 7.11e-12 | 67.0 |
| MS.gene065201.t1 | AT5G08390 | 24.583 | 240 | 160 | 8 | 68 | 299 | 13 | 239 | 9.64e-11 | 63.5 |
| MS.gene065201.t1 | AT3G16650 | 22.876 | 306 | 200 | 9 | 13 | 309 | 159 | 437 | 1.12e-11 | 66.2 |
Find 75 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACATCTTGTCTGATATTTC+AGG | 0.182516 | 8.1:-36236246 | None:intergenic |
| TATTACCGGATGGAAATTTA+AGG | 0.288149 | 8.1:-36236945 | None:intergenic |
| GTCAACAGACCGAGTATAAA+AGG | 0.294586 | 8.1:+36236212 | MS.gene065201:intron |
| GCATACCGCGTAAGCATATA+TGG | 0.318779 | 8.1:-36237312 | None:intergenic |
| GTCTATAGGTTTGCTATGAT+TGG | 0.329481 | 8.1:+36240182 | MS.gene065201:intron |
| CCGATCAACATGTCTTCTTT+CGG | 0.336166 | 8.1:+36232966 | None:intergenic |
| TGAAGTAGCGACGAGGAAAT+TGG | 0.342617 | 8.1:-36233163 | None:intergenic |
| ATTTCCTCGTCGCTACTTCA+TGG | 0.370098 | 8.1:+36233166 | MS.gene065201:CDS |
| TTCAATTTCGCATGATCAAC+CGG | 0.383740 | 8.1:+36233336 | MS.gene065201:CDS |
| ATTACCTCATAGGATTTGTT+TGG | 0.383763 | 8.1:-36233011 | None:intergenic |
| ATGTAGTTCTTTGCTGTAGT+TGG | 0.387737 | 8.1:-36240229 | None:intergenic |
| GCGACGAGGAAATTGGCTTT+AGG | 0.387932 | 8.1:-36233156 | None:intergenic |
| AATGCATAGCATTACCTCAT+AGG | 0.420572 | 8.1:-36233021 | None:intergenic |
| GACATTGTATAACATCGATC+AGG | 0.430312 | 8.1:-36235263 | None:intergenic |
| ATTGAATGTACCACAAGGTA+TGG | 0.430485 | 8.1:-36237279 | None:intergenic |
| AGCATAACCGCTTCCGACTA+AGG | 0.430681 | 8.1:-36241832 | None:intergenic |
| TGCTCTGCTTGGAAAGATGA+TGG | 0.442296 | 8.1:+36233484 | MS.gene065201:CDS |
| GCGTTAGGTATTGGGATACT+AGG | 0.449325 | 8.1:+36235210 | MS.gene065201:intron |
| AGAAAGTAATGAGATATATT+CGG | 0.449681 | 8.1:+36236916 | MS.gene065201:CDS |
| TTCAGGAGGTTGTGACAAGC+AGG | 0.466291 | 8.1:+36233519 | MS.gene065201:CDS |
| ATCCATGCAATCTCTTTGAT+AGG | 0.470416 | 8.1:-36233601 | None:intergenic |
| TGTGGTACATTCAATAATGA+TGG | 0.470459 | 8.1:+36237287 | MS.gene065201:CDS |
| TCATGCGAAATTGAAGCCTT+AGG | 0.481510 | 8.1:-36233328 | None:intergenic |
| CTTCAACTTGCAGAATCCTC+AGG | 0.486798 | 8.1:+36235337 | MS.gene065201:CDS |
| GGGAGTTGTTGAGTATGCAC+TGG | 0.487395 | 8.1:-36235242 | None:intergenic |
| CGCTACTTCATGGGACAACC+AGG | 0.487972 | 8.1:+36233176 | MS.gene065201:CDS |
| ACATCTTGTCTGATATTTCA+GGG | 0.489867 | 8.1:-36236245 | None:intergenic |
| TTTCCTCGTCGCTACTTCAT+GGG | 0.497853 | 8.1:+36233167 | MS.gene065201:CDS |
| AATTGAAGCCTTAGGTGTAC+TGG | 0.501065 | 8.1:-36233320 | None:intergenic |
| CAAAGAGATTGCATGGATAC+CGG | 0.503787 | 8.1:+36233606 | MS.gene065201:CDS |
| TGCTTTGTTGTGCATCATCA+AGG | 0.506986 | 8.1:-36236868 | None:intergenic |
| TTTAGGACTGAAAGAAAGAC+TGG | 0.514761 | 8.1:-36233139 | None:intergenic |
| CTCCTATCAAAGAGATTGCA+TGG | 0.517842 | 8.1:+36233599 | MS.gene065201:CDS |
| CAACTCCTTAAATTTCCATC+CGG | 0.521958 | 8.1:+36236940 | MS.gene065201:CDS |
| AGAAAGACTGGAAATAGAAT+CGG | 0.522597 | 8.1:-36233127 | None:intergenic |
| GGCTCAATAGAGGGAAGGGT+TGG | 0.523184 | 8.1:+36236836 | MS.gene065201:CDS |
| CTGTAGGTTGGCTCAATAGA+GGG | 0.523662 | 8.1:+36236827 | MS.gene065201:intron |
| CCTGTAGGTTGGCTCAATAG+AGG | 0.529343 | 8.1:+36236826 | MS.gene065201:intron |
| TGTTGGGAAATATCAAAGAA+TGG | 0.540936 | 8.1:+36233286 | MS.gene065201:CDS |
| TTTGCAACTGCTGGATCTGA+CGG | 0.543216 | 8.1:+36237085 | MS.gene065201:CDS |
| GTTTGCTATGATTGGAGCAA+AGG | 0.546194 | 8.1:+36240190 | MS.gene065201:CDS |
| GAGACATCCTCTGATGGTTG+TGG | 0.546885 | 8.1:+36235289 | MS.gene065201:CDS |
| AAATCCAAACAAATCCTATG+AGG | 0.547226 | 8.1:+36233007 | MS.gene065201:CDS |
| TGTTGTTACCAGTACACCTA+AGG | 0.556047 | 8.1:+36233312 | MS.gene065201:CDS |
| TCATGCATAGCAACGGTCAC+GGG | 0.559382 | 8.1:-36233574 | None:intergenic |
| TTGAATGTACCACAAGGTAT+GGG | 0.565862 | 8.1:-36237278 | None:intergenic |
| ATCATGCATAGCAACGGTCA+CGG | 0.570899 | 8.1:-36233575 | None:intergenic |
| TTACACACGTGCACCTTAGT+CGG | 0.589243 | 8.1:+36241819 | MS.gene065201:CDS |
| GAAGACAATCAGATTCCTAT+CGG | 0.589539 | 8.1:-36235318 | None:intergenic |
| GTGGCTAAAAGACTCATCTC+CGG | 0.590432 | 8.1:-36233625 | None:intergenic |
| CATATATATGCACTTACCAC+AGG | 0.593393 | 8.1:+36240249 | MS.gene065201:CDS |
| AAGACTGGAAATAGAATCGG+TGG | 0.606098 | 8.1:-36233124 | None:intergenic |
| GGTTGGCTCAATAGAGGGAA+GGG | 0.612759 | 8.1:+36236832 | MS.gene065201:intron |
| AGACATCCTCTGATGGTTGT+GGG | 0.614239 | 8.1:+36235290 | MS.gene065201:CDS |
| AGGTTGGCTCAATAGAGGGA+AGG | 0.615075 | 8.1:+36236831 | MS.gene065201:intron |
| ACGTGCACCTTAGTCGGAAG+CGG | 0.619672 | 8.1:+36241825 | MS.gene065201:CDS |
| TTGTTGAGTATGCACTGGGT+TGG | 0.621738 | 8.1:-36235237 | None:intergenic |
| GCAGTTCCCACAACCATCAG+AGG | 0.624752 | 8.1:-36235296 | None:intergenic |
| GGAGTTGTTGAGTATGCACT+GGG | 0.626401 | 8.1:-36235241 | None:intergenic |
| ACATTGTATAACATCGATCA+GGG | 0.631495 | 8.1:-36235262 | None:intergenic |
| CTATGATTGGAGCAAAGGTG+CGG | 0.636918 | 8.1:+36240195 | MS.gene065201:CDS |
| CATCTTGTCTGATATTTCAG+GGG | 0.643367 | 8.1:-36236244 | None:intergenic |
| GGACAGTAAACAGAGACTCA+AGG | 0.644768 | 8.1:+36237129 | MS.gene065201:CDS |
| AGGAGCATCATGCATAGCAA+CGG | 0.647992 | 8.1:-36233581 | None:intergenic |
| ATATCTCATTACTTTCTCTG+TGG | 0.654168 | 8.1:-36236910 | None:intergenic |
| GGTTGCCCTCCAGACAAAAG+CGG | 0.656877 | 8.1:-36233553 | None:intergenic |
| TGTAGTCAGCCCATACCTTG+TGG | 0.658844 | 8.1:+36237269 | MS.gene065201:CDS |
| GTCAGTGAGACATCCTCTGA+TGG | 0.664484 | 8.1:+36235283 | MS.gene065201:CDS |
| GTGACAAGCAGGCTAAGATG+TGG | 0.674018 | 8.1:+36233530 | MS.gene065201:CDS |
| CTGGAAATAGAATCGGTGGG+TGG | 0.693399 | 8.1:-36233120 | None:intergenic |
| TGGTTCCATATATGCTTACG+CGG | 0.709671 | 8.1:+36237307 | MS.gene065201:CDS |
| TGGTTGTGGGAACTGCCGAT+AGG | 0.714062 | 8.1:+36235303 | MS.gene065201:CDS |
| TCATTATTGAATGTACCACA+AGG | 0.728862 | 8.1:-36237284 | None:intergenic |
| TGTCCCATGAAGTAGCGACG+AGG | 0.750186 | 8.1:-36233170 | None:intergenic |
| AGACTGGAAATAGAATCGGT+GGG | 0.750574 | 8.1:-36233123 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 36232975 | 36241857 | 36232975 | ID=MS.gene065201 |
| chr8.1 | mRNA | 36232975 | 36241857 | 36232975 | ID=MS.gene065201.t1;Parent=MS.gene065201 |
| chr8.1 | exon | 36232975 | 36233028 | 36232975 | ID=MS.gene065201.t1.exon1;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36232975 | 36233028 | 36232975 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36233111 | 36233197 | 36233111 | ID=MS.gene065201.t1.exon2;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36233111 | 36233197 | 36233111 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36233280 | 36233357 | 36233280 | ID=MS.gene065201.t1.exon3;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36233280 | 36233357 | 36233280 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36233478 | 36233671 | 36233478 | ID=MS.gene065201.t1.exon4;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36233478 | 36233671 | 36233478 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36235217 | 36235358 | 36235217 | ID=MS.gene065201.t1.exon5;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36235217 | 36235358 | 36235217 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36236220 | 36236300 | 36236220 | ID=MS.gene065201.t1.exon6;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36236220 | 36236300 | 36236220 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36236833 | 36236961 | 36236833 | ID=MS.gene065201.t1.exon7;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36236833 | 36236961 | 36236833 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36237073 | 36237150 | 36237073 | ID=MS.gene065201.t1.exon8;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36237073 | 36237150 | 36237073 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36237257 | 36237328 | 36237257 | ID=MS.gene065201.t1.exon9;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36237257 | 36237328 | 36237257 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36240190 | 36240270 | 36240190 | ID=MS.gene065201.t1.exon10;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36240190 | 36240270 | 36240190 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| chr8.1 | exon | 36241798 | 36241857 | 36241798 | ID=MS.gene065201.t1.exon11;Parent=MS.gene065201.t1 |
| chr8.1 | CDS | 36241798 | 36241857 | 36241798 | ID=cds.MS.gene065201.t1;Parent=MS.gene065201.t1 |
| Gene Sequence |
| Protein sequence |
