Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065358.t1 | XP_003625486.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065358.t1 | Q2HT99 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.0e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene065358 | 0.842734 | 2.16E-58 | -1.69E-46 |
| MS.gene050563 | MS.gene065358 | 0.808841 | 2.54E-50 | -1.69E-46 |
| MS.gene050584 | MS.gene065358 | 0.870444 | 1.45E-66 | -1.69E-46 |
| MS.gene050585 | MS.gene065358 | 0.83021 | 3.34E-55 | -1.69E-46 |
| MS.gene050587 | MS.gene065358 | 0.835849 | 1.32E-56 | -1.69E-46 |
| MS.gene05157 | MS.gene065358 | 0.823151 | 1.62E-53 | -1.69E-46 |
| MS.gene051789 | MS.gene065358 | 0.853328 | 2.57E-61 | -1.69E-46 |
| MS.gene052142 | MS.gene065358 | 0.847845 | 8.94E-60 | -1.69E-46 |
| MS.gene053074 | MS.gene065358 | 0.850543 | 1.59E-60 | -1.69E-46 |
| MS.gene053383 | MS.gene065358 | 0.840468 | 8.54E-58 | -1.69E-46 |
| MS.gene05456 | MS.gene065358 | 0.853024 | 3.14E-61 | -1.69E-46 |
| MS.gene055359 | MS.gene065358 | 0.840089 | 1.07E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene065358 | 0.880566 | 4.94E-70 | -1.69E-46 |
| MS.gene059768 | MS.gene065358 | 0.804514 | 2.08E-49 | -1.69E-46 |
| MS.gene060691 | MS.gene065358 | 0.811516 | 6.73E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065358.t1 | MTR_7g099670 | 98.077 | 104 | 2 | 0 | 1 | 104 | 1 | 104 | 3.16e-60 | 179 |
| MS.gene065358.t1 | MTR_1g069085 | 68.000 | 50 | 14 | 2 | 1 | 49 | 1 | 49 | 5.67e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGGTGGACTTGGTGGTTT+GGG | 0.209445 | 7.4:+10525000 | MS.gene065358:CDS |
| ACATTTGCTGGTGGTTATTC+AGG | 0.231052 | 7.4:+10524904 | MS.gene065358:CDS |
| GGAGGACTTGGTGGGGTTGG+TGG | 0.235350 | 7.4:+10525021 | MS.gene065358:CDS |
| CCTAGGTGGACTTGGTGGTT+TGG | 0.244833 | 7.4:+10524999 | MS.gene065358:CDS |
| CTTGGTGGTTTGGGAGGACT+TGG | 0.272357 | 7.4:+10525009 | MS.gene065358:CDS |
| GGACTTGGTGGGGTTGGTGG+TGG | 0.293034 | 7.4:+10525024 | MS.gene065358:CDS |
| GGTGGAGCTGGTGGTGGTGT+TGG | 0.293326 | 7.4:+10525042 | MS.gene065358:CDS |
| GGTGGAATCGGTGGTGATTT+AGG | 0.312961 | 7.4:+10524964 | MS.gene065358:CDS |
| GGTGGTTTGGGAGGACTTGG+TGG | 0.314590 | 7.4:+10525012 | MS.gene065358:CDS |
| TTGGGAGGACTTGGTGGGGT+TGG | 0.314725 | 7.4:+10525018 | MS.gene065358:CDS |
| GGTGGGGTTGGTGGTGGAGC+TGG | 0.316689 | 7.4:+10525030 | MS.gene065358:CDS |
| GTTGGTGGTGGAGCTGGTGG+TGG | 0.321018 | 7.4:+10525036 | MS.gene065358:CDS |
| GGAAACAATGGACTTCCTTT+TGG | 0.322482 | 7.4:+10524931 | MS.gene065358:CDS |
| TTAGGTGGTGGTGGTGGCCT+AGG | 0.323708 | 7.4:+10524982 | MS.gene065358:CDS |
| GGTGGTGGCCTAGGTGGACT+TGG | 0.331166 | 7.4:+10524991 | MS.gene065358:CDS |
| GGTGGTGTTGGAGGTGGTGT+TGG | 0.331290 | 7.4:+10525054 | MS.gene065358:CDS |
| GGGGTTGGTGGTGGAGCTGG+TGG | 0.333006 | 7.4:+10525033 | MS.gene065358:CDS |
| GCTGGTGGTTATTCAGGAAT+AGG | 0.341133 | 7.4:+10524910 | MS.gene065358:CDS |
| GTTGGAGGTGGTGTTGGAAC+TGG | 0.344227 | 7.4:+10525060 | MS.gene065358:CDS |
| CAAATGTCATGAAGTTCTTC+TGG | 0.354882 | 7.4:-10524888 | None:intergenic |
| GCTGGTGGTGGTGTTGGAGG+TGG | 0.373109 | 7.4:+10525048 | MS.gene065358:CDS |
| AAGAACTTCATGACATTTGC+TGG | 0.375624 | 7.4:+10524892 | MS.gene065358:CDS |
| GGAGCTGGTGGTGGTGTTGG+AGG | 0.379910 | 7.4:+10525045 | MS.gene065358:CDS |
| GGTGGTGTTGGAACTGGTGT+TGG | 0.385912 | 7.4:+10525066 | MS.gene065358:CDS |
| GGTGGAATTGGAGGTGGAAT+CGG | 0.386910 | 7.4:+10524952 | MS.gene065358:CDS |
| GTGGTTTGGGAGGACTTGGT+GGG | 0.431797 | 7.4:+10525013 | MS.gene065358:CDS |
| TGGTTTGGGAGGACTTGGTG+GGG | 0.448017 | 7.4:+10525014 | MS.gene065358:CDS |
| GGTGTTGGAACTGGTGTTGG+TGG | 0.458137 | 7.4:+10525069 | MS.gene065358:CDS |
| AAAGTGGTGTATGTTGTTGT+TGG | 0.463389 | 7.4:+10524801 | MS.gene065358:CDS |
| GGTGGCCTAGGTGGACTTGG+TGG | 0.480801 | 7.4:+10524994 | MS.gene065358:CDS |
| GGTGGACTTGGTGGTTTGGG+AGG | 0.508576 | 7.4:+10525003 | MS.gene065358:CDS |
| GTTGGTTCTTGCTCTTGTTG+TGG | 0.516173 | 7.4:+10524819 | MS.gene065358:CDS |
| GGTGGTGGTGGTGGCCTAGG+TGG | 0.516203 | 7.4:+10524985 | MS.gene065358:CDS |
| ACCAACTGATGCTGGTTTGA+AGG | 0.518003 | 7.4:+10524864 | MS.gene065358:CDS |
| GGTGATTTAGGTGGTGGTGG+TGG | 0.527759 | 7.4:+10524976 | MS.gene065358:CDS |
| ATCGGTGGTGATTTAGGTGG+TGG | 0.527781 | 7.4:+10524970 | MS.gene065358:CDS |
| CCACCTCCAATTCCACCAAA+AGG | 0.542402 | 7.4:-10524946 | None:intergenic |
| GGAATTGGAGGTGGAATCGG+TGG | 0.552447 | 7.4:+10524955 | MS.gene065358:CDS |
| GGTGGTGATTTAGGTGGTGG+TGG | 0.559090 | 7.4:+10524973 | MS.gene065358:CDS |
| GAATACTGATCTTAAGGGAA+AGG | 0.564229 | 7.4:-10525099 | None:intergenic |
| AGAAATGTACCAACTGATGC+TGG | 0.575990 | 7.4:+10524856 | MS.gene065358:CDS |
| TATTCAGGAATAGGAAACAA+TGG | 0.605549 | 7.4:+10524919 | MS.gene065358:CDS |
| GGAATCGGTGGTGATTTAGG+TGG | 0.612801 | 7.4:+10524967 | MS.gene065358:CDS |
| GAGTGAGAGATATGGCAAAG+TGG | 0.614546 | 7.4:+10524785 | None:intergenic |
| AACTTCATGACATTTGCTGG+TGG | 0.621619 | 7.4:+10524895 | MS.gene065358:CDS |
| TCCTTCAAACCAGCATCAGT+TGG | 0.622405 | 7.4:-10524865 | None:intergenic |
| CCAAACCACCAAGTCCACCT+AGG | 0.637542 | 7.4:-10524999 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCAGGAATAGGAAACAA+TGG | + | chr7.4:10524919-10524938 | MS.gene065358:CDS | 30.0% | |
| AAAGTGGTGTATGTTGTTGT+TGG | + | chr7.4:10524801-10524820 | MS.gene065358:CDS | 35.0% | |
| AAGAACTTCATGACATTTGC+TGG | + | chr7.4:10524892-10524911 | MS.gene065358:CDS | 35.0% | |
| CAAATGTCATGAAGTTCTTC+TGG | - | chr7.4:10524891-10524910 | None:intergenic | 35.0% | |
| AGAAATGTACCAACTGATGC+TGG | + | chr7.4:10524856-10524875 | MS.gene065358:CDS | 40.0% | |
| ! | AACTTCATGACATTTGCTGG+TGG | + | chr7.4:10524895-10524914 | MS.gene065358:CDS | 40.0% |
| ! | ACATTTGCTGGTGGTTATTC+AGG | + | chr7.4:10524904-10524923 | MS.gene065358:CDS | 40.0% |
| !! | AACAATGGACTTCCTTTTGG+TGG | + | chr7.4:10524934-10524953 | MS.gene065358:CDS | 40.0% |
| !! | GGAAACAATGGACTTCCTTT+TGG | + | chr7.4:10524931-10524950 | MS.gene065358:CDS | 40.0% |
| GCTGGTGGTTATTCAGGAAT+AGG | + | chr7.4:10524910-10524929 | MS.gene065358:CDS | 45.0% | |
| TCCTTCAAACCAGCATCAGT+TGG | - | chr7.4:10524868-10524887 | None:intergenic | 45.0% | |
| ! | CTTCCTTTTGGTGGAATTGG+AGG | + | chr7.4:10524943-10524962 | MS.gene065358:CDS | 45.0% |
| ! | GGACTTCCTTTTGGTGGAAT+TGG | + | chr7.4:10524940-10524959 | MS.gene065358:CDS | 45.0% |
| ! | GTTGGTTCTTGCTCTTGTTG+TGG | + | chr7.4:10524819-10524838 | MS.gene065358:CDS | 45.0% |
| !! | ACCAACTGATGCTGGTTTGA+AGG | + | chr7.4:10524864-10524883 | MS.gene065358:CDS | 45.0% |
| ATCGGTGGTGATTTAGGTGG+TGG | + | chr7.4:10524970-10524989 | MS.gene065358:CDS | 50.0% | |
| CCACCTCCAATTCCACCAAA+AGG | - | chr7.4:10524949-10524968 | None:intergenic | 50.0% | |
| GGAATCGGTGGTGATTTAGG+TGG | + | chr7.4:10524967-10524986 | MS.gene065358:CDS | 50.0% | |
| GGTGGAATCGGTGGTGATTT+AGG | + | chr7.4:10524964-10524983 | MS.gene065358:CDS | 50.0% | |
| GGTGGAATTGGAGGTGGAAT+CGG | + | chr7.4:10524952-10524971 | MS.gene065358:CDS | 50.0% | |
| ! | CCTTTTGGTGGAATTGGAGG+TGG | + | chr7.4:10524946-10524965 | MS.gene065358:CDS | 50.0% |
| !! | CTAGGTGGACTTGGTGGTTT+GGG | + | chr7.4:10525000-10525019 | MS.gene065358:CDS | 50.0% |
| CCAAACCACCAAGTCCACCT+AGG | - | chr7.4:10525002-10525021 | None:intergenic | 55.0% | |
| GGAATTGGAGGTGGAATCGG+TGG | + | chr7.4:10524955-10524974 | MS.gene065358:CDS | 55.0% | |
| ! | CTTGGTGGTTTGGGAGGACT+TGG | + | chr7.4:10525009-10525028 | MS.gene065358:CDS | 55.0% |
| ! | GGTGGTGATTTAGGTGGTGG+TGG | + | chr7.4:10524973-10524992 | MS.gene065358:CDS | 55.0% |
| ! | GGTGGTGTTGGAACTGGTGT+TGG | + | chr7.4:10525066-10525085 | MS.gene065358:CDS | 55.0% |
| ! | GGTGTTGGAACTGGTGTTGG+TGG | + | chr7.4:10525069-10525088 | MS.gene065358:CDS | 55.0% |
| ! | GTGGTTTGGGAGGACTTGGT+GGG | + | chr7.4:10525013-10525032 | MS.gene065358:CDS | 55.0% |
| ! | GTTGGAGGTGGTGTTGGAAC+TGG | + | chr7.4:10525060-10525079 | MS.gene065358:CDS | 55.0% |
| !! | CCTAGGTGGACTTGGTGGTT+TGG | + | chr7.4:10524999-10525018 | MS.gene065358:CDS | 55.0% |
| !! | GGTGATTTAGGTGGTGGTGG+TGG | + | chr7.4:10524976-10524995 | MS.gene065358:CDS | 55.0% |
| !! | TGGTTTGGGAGGACTTGGTG+GGG | + | chr7.4:10525014-10525033 | MS.gene065358:CDS | 55.0% |
| ! | GGTGGTGTTGGAGGTGGTGT+TGG | + | chr7.4:10525054-10525073 | MS.gene065358:CDS | 60.0% |
| ! | GGTGGTTTGGGAGGACTTGG+TGG | + | chr7.4:10525012-10525031 | MS.gene065358:CDS | 60.0% |
| !! | GGTGGACTTGGTGGTTTGGG+AGG | + | chr7.4:10525003-10525022 | MS.gene065358:CDS | 60.0% |
| !! | TTAGGTGGTGGTGGTGGCCT+AGG | + | chr7.4:10524982-10525001 | MS.gene065358:CDS | 60.0% |
| !! | TTGGGAGGACTTGGTGGGGT+TGG | + | chr7.4:10525018-10525037 | MS.gene065358:CDS | 60.0% |
| ! | GGTGGCCTAGGTGGACTTGG+TGG | + | chr7.4:10524994-10525013 | MS.gene065358:CDS | 65.0% |
| ! | GGTGGTGGCCTAGGTGGACT+TGG | + | chr7.4:10524991-10525010 | MS.gene065358:CDS | 65.0% |
| !! | GCTGGTGGTGGTGTTGGAGG+TGG | + | chr7.4:10525048-10525067 | MS.gene065358:CDS | 65.0% |
| !! | GGACTTGGTGGGGTTGGTGG+TGG | + | chr7.4:10525024-10525043 | MS.gene065358:CDS | 65.0% |
| !! | GGAGCTGGTGGTGGTGTTGG+AGG | + | chr7.4:10525045-10525064 | MS.gene065358:CDS | 65.0% |
| !! | GGAGGACTTGGTGGGGTTGG+TGG | + | chr7.4:10525021-10525040 | MS.gene065358:CDS | 65.0% |
| !! | GGTGGAGCTGGTGGTGGTGT+TGG | + | chr7.4:10525042-10525061 | MS.gene065358:CDS | 65.0% |
| !! | GTTGGTGGTGGAGCTGGTGG+TGG | + | chr7.4:10525036-10525055 | MS.gene065358:CDS | 65.0% |
| !! | GGGGTTGGTGGTGGAGCTGG+TGG | + | chr7.4:10525033-10525052 | MS.gene065358:CDS | 70.0% |
| !! | GGTGGGGTTGGTGGTGGAGC+TGG | + | chr7.4:10525030-10525049 | MS.gene065358:CDS | 70.0% |
| !! | GGTGGTGGTGGTGGCCTAGG+TGG | + | chr7.4:10524985-10525004 | MS.gene065358:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10524796 | 10525110 | 10524796 | ID=MS.gene065358 |
| chr7.4 | mRNA | 10524796 | 10525110 | 10524796 | ID=MS.gene065358.t1;Parent=MS.gene065358 |
| chr7.4 | exon | 10524796 | 10525110 | 10524796 | ID=MS.gene065358.t1.exon1;Parent=MS.gene065358.t1 |
| chr7.4 | CDS | 10524796 | 10525110 | 10524796 | ID=cds.MS.gene065358.t1;Parent=MS.gene065358.t1 |
| Gene Sequence |
| Protein sequence |
