Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065971.t1 | AET04334.2 | 97.2 | 72 | 2 | 0 | 1 | 72 | 1 | 72 | 6.20E-33 | 149.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065971.t1 | I3SS67 | 97.2 | 72 | 2 | 0 | 1 | 72 | 27 | 98 | 4.5e-33 | 149.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050444 | MS.gene065971 | -0.80392 | 2.77E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene065971 | -0.858449 | 8.17E-63 | -1.69E-46 |
| MS.gene051317 | MS.gene065971 | -0.814034 | 1.89E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene065971 | -0.834281 | 3.28E-56 | -1.69E-46 |
| MS.gene055750 | MS.gene065971 | -0.821807 | 3.32E-53 | -1.69E-46 |
| MS.gene055751 | MS.gene065971 | -0.846196 | 2.53E-59 | -1.69E-46 |
| MS.gene05589 | MS.gene065971 | -0.81063 | 1.05E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene065971 | -0.802502 | 5.44E-49 | -1.69E-46 |
| MS.gene056618 | MS.gene065971 | 0.841724 | 3.99E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene065971 | -0.811204 | 7.87E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene065971 | -0.814417 | 1.56E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065971.t1 | MTR_8g087660 | 97.222 | 72 | 2 | 0 | 1 | 72 | 1 | 72 | 3.02e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTAAATCTGAAGCTTTA+AGG | 0.110020 | 4.3:-13352816 | MS.gene065971:CDS |
| AGCTTTAAGGTTGCCTCACC+AGG | 0.416451 | 4.3:-13352803 | MS.gene065971:CDS |
| CCAACTCTCCACAGTTGATA+AGG | 0.434883 | 4.3:+13352911 | None:intergenic |
| CTCAGAACTCCAAGTGCAGC+TGG | 0.436184 | 4.3:-13352870 | MS.gene065971:CDS |
| ACTGGTGCACCAGCTGCACT+TGG | 0.490099 | 4.3:+13352861 | None:intergenic |
| AAAAGATAGATCTCATAAAC+GGG | 0.490121 | 4.3:+13352762 | None:intergenic |
| CCTTGTTTGTCAAATGGTGG+TGG | 0.499531 | 4.3:-13352983 | None:intergenic |
| CTCTCCACAGTTGATAAGGA+TGG | 0.512074 | 4.3:+13352915 | None:intergenic |
| CCTTATCAACTGTGGAGAGT+TGG | 0.513468 | 4.3:-13352911 | MS.gene065971:CDS |
| CAAATGGTGGTGGCTGAAGA+TGG | 0.538267 | 4.3:-13352973 | MS.gene065971:CDS |
| AGTATCATCAAGATCTTGAC+TGG | 0.560635 | 4.3:+13352843 | None:intergenic |
| TGGGCCCACAAGAGAAAACC+TGG | 0.594100 | 4.3:+13352785 | None:intergenic |
| TCTCCACAGTTGATAAGGAT+GGG | 0.596020 | 4.3:+13352916 | None:intergenic |
| AGATAGATCTCATAAACGGG+TGG | 0.606925 | 4.3:+13352765 | None:intergenic |
| CCACAAGAGAAAACCTGGTG+AGG | 0.632813 | 4.3:+13352790 | None:intergenic |
| GATAGATCTCATAAACGGGT+GGG | 0.637322 | 4.3:+13352766 | None:intergenic |
| TGGCTGAAGATGGTTCATCA+AGG | 0.647563 | 4.3:-13352963 | MS.gene065971:CDS |
| AGACCCATCCTTATCAACTG+TGG | 0.653046 | 4.3:-13352919 | MS.gene065971:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTCTAAATCTGAAGCTTTA+AGG | - | chr4.3:13352928-13352947 | MS.gene065971:CDS | 30.0% |
| AGTATCATCAAGATCTTGAC+TGG | + | chr4.3:13352904-13352923 | None:intergenic | 35.0% | |
| !! | TCTCCACAGTTGATAAGGAT+GGG | + | chr4.3:13352831-13352850 | None:intergenic | 40.0% |
| AGACCCATCCTTATCAACTG+TGG | - | chr4.3:13352825-13352844 | MS.gene065971:CDS | 45.0% | |
| CCTTATCAACTGTGGAGAGT+TGG | - | chr4.3:13352833-13352852 | MS.gene065971:CDS | 45.0% | |
| TGGCTGAAGATGGTTCATCA+AGG | - | chr4.3:13352781-13352800 | MS.gene065971:CDS | 45.0% | |
| ! | CCAACTCTCCACAGTTGATA+AGG | + | chr4.3:13352836-13352855 | None:intergenic | 45.0% |
| ! | CTCACCAGGTTTTCTCTTGT+GGG | - | chr4.3:13352955-13352974 | MS.gene065971:CDS | 45.0% |
| ! | GATAAGGATGGGTCTTTTGC+TGG | + | chr4.3:13352820-13352839 | None:intergenic | 45.0% |
| !! | CTCTCCACAGTTGATAAGGA+TGG | + | chr4.3:13352832-13352851 | None:intergenic | 45.0% |
| !! | GGTCTTTTGCTGGCTGAAAA+GGG | + | chr4.3:13352810-13352829 | None:intergenic | 45.0% |
| CCACAAGAGAAAACCTGGTG+AGG | + | chr4.3:13352957-13352976 | None:intergenic | 50.0% | |
| ! | CCTCACCAGGTTTTCTCTTG+TGG | - | chr4.3:13352954-13352973 | MS.gene065971:CDS | 50.0% |
| !! | AGCTTTAAGGTTGCCTCACC+AGG | - | chr4.3:13352941-13352960 | MS.gene065971:CDS | 50.0% |
| !! | GGGTCTTTTGCTGGCTGAAA+AGG | + | chr4.3:13352811-13352830 | None:intergenic | 50.0% |
| CTCAGAACTCCAAGTGCAGC+TGG | - | chr4.3:13352874-13352893 | MS.gene065971:CDS | 55.0% | |
| TGGGCCCACAAGAGAAAACC+TGG | + | chr4.3:13352962-13352981 | None:intergenic | 55.0% | |
| ACTGGTGCACCAGCTGCACT+TGG | + | chr4.3:13352886-13352905 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13352774 | 13352992 | 13352774 | ID=MS.gene065971 |
| chr4.3 | mRNA | 13352774 | 13352992 | 13352774 | ID=MS.gene065971.t1;Parent=MS.gene065971 |
| chr4.3 | exon | 13352774 | 13352992 | 13352774 | ID=MS.gene065971.t1.exon1;Parent=MS.gene065971.t1 |
| chr4.3 | CDS | 13352774 | 13352992 | 13352774 | ID=cds.MS.gene065971.t1;Parent=MS.gene065971.t1 |
| Gene Sequence |
| Protein sequence |
