Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067271.t1 | XP_013445262.1 | 77.5 | 426 | 71 | 1 | 1 | 426 | 11 | 411 | 1.60E-67 | 266.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067271.t1 | D9UC01 | 35.6 | 104 | 67 | 0 | 14 | 117 | 16 | 119 | 3.9e-14 | 80.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067271.t1 | A0A072TR16 | 77.5 | 426 | 71 | 1 | 1 | 426 | 11 | 411 | 1.1e-67 | 266.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049698 | MS.gene067271 | 0.807113 | 5.92E-50 | -1.69E-46 |
| MS.gene05017 | MS.gene067271 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene058351 | MS.gene067271 | 0.80558 | 1.25E-49 | -1.69E-46 |
| MS.gene058909 | MS.gene067271 | 0.85914 | 5.08E-63 | -1.69E-46 |
| MS.gene058912 | MS.gene067271 | 0.857132 | 2.01E-62 | -1.69E-46 |
| MS.gene05894 | MS.gene067271 | 0.813076 | 3.07E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTAAATCTTTATTATTTGA+AGG | + | 54857:3705-3724 | MS.gene067271:intergenic | 15.0% |
| !! | TATCATATGAATTGTACTTA+CGG | + | 54857:3262-3281 | MS.gene067271:intergenic | 20.0% |
| !!! | TATTTCTGTTTTGATTTTGT+AGG | - | 54857:3435-3454 | MS.gene067271:CDS | 20.0% |
| !!! | TCAAAACAGAAATATAGTTT+TGG | + | 54857:3429-3448 | MS.gene067271:intergenic | 20.0% |
| ! | AAAGTCAAAATTTGTGTGTA+TGG | - | 54857:3386-3405 | MS.gene067271:CDS | 25.0% |
| ! | AATTTAGAGGAAAAGATAGT+AGG | - | 54857:4242-4261 | MS.gene067271:CDS | 25.0% |
| ! | CAAAACACAAACAAATGAAA+AGG | - | 54857:4160-4179 | MS.gene067271:CDS | 25.0% |
| ! | TAAAGATTTAGATGAAGCAA+AGG | - | 54857:3713-3732 | MS.gene067271:CDS | 25.0% |
| !! | ACCAATTTCTCTATTGTTTT+TGG | + | 54857:4212-4231 | MS.gene067271:intergenic | 25.0% |
| !! | CTTTTCATCTATTTGTGTTT+TGG | + | 54857:4087-4106 | MS.gene067271:intergenic | 25.0% |
| !! | CTTTTCATTTGTTTGTGTTT+TGG | + | 54857:4162-4181 | MS.gene067271:intergenic | 25.0% |
| !! | CTTTTCCTCTAAATTTTCTT+TGG | + | 54857:4237-4256 | MS.gene067271:intergenic | 25.0% |
| !! | TCATTGCTAATTGTTTCTTT+AGG | + | 54857:3879-3898 | MS.gene067271:intergenic | 25.0% |
| !! | TGATTTTGTAGGTTTTACAA+AGG | - | 54857:3446-3465 | MS.gene067271:CDS | 25.0% |
| !!! | AATTTTCTCCATTTGTTAAC+TGG | + | 54857:3537-3556 | MS.gene067271:intergenic | 25.0% |
| !!! | CTTTTCATCTATTTGCTTTT+TGG | + | 54857:3961-3980 | MS.gene067271:intergenic | 25.0% |
| !!! | TTTTGGTTTGATTATATAGC+AGG | + | 54857:3412-3431 | MS.gene067271:intergenic | 25.0% |
| AACAAATGAAAAGGCTCAAA+AGG | - | 54857:4169-4188 | MS.gene067271:CDS | 30.0% | |
| AATAGATGAAAAGCCTCAAA+AGG | - | 54857:3968-3987 | MS.gene067271:CDS | 30.0% | |
| AATGACAATAACCCTCAAAA+GGG | - | 54857:3894-3913 | MS.gene067271:CDS | 30.0% | |
| ACAAATGAAAAGGCTCAAAA+GGG | - | 54857:4170-4189 | MS.gene067271:CDS | 30.0% | |
| AGACATTTAGCTGAAAATGT+AGG | - | 54857:4542-4561 | MS.gene067271:CDS | 30.0% | |
| ATAGATGAAAAGCCTCAAAA+GGG | - | 54857:3969-3988 | MS.gene067271:CDS | 30.0% | |
| ATAGATGAAAAGCCTCAAAA+TGG | - | 54857:3969-3988 | MS.gene067271:CDS | 30.0% | |
| GCCAAAAACAATAGAGAAAT+TGG | - | 54857:4208-4227 | MS.gene067271:CDS | 30.0% | |
| GGTTACCAAAGAAAATTTAG+AGG | - | 54857:4229-4248 | MS.gene067271:CDS | 30.0% | |
| TCAAAATGGTAAAGAGGATT+TGG | - | 54857:4109-4128 | MS.gene067271:CDS | 30.0% | |
| ! | AAATCCTCTTTACCATTTTG+AGG | + | 54857:4110-4129 | MS.gene067271:intergenic | 30.0% |
| ! | GTTTCTTTAGGAATTTGAGA+TGG | + | 54857:3867-3886 | MS.gene067271:intergenic | 30.0% |
| !! | ATGCCTTTAACTTGTTTACA+TGG | - | 54857:3227-3246 | MS.gene067271:CDS | 30.0% |
| !! | GTTGATTCTTCCAATTTTTG+TGG | + | 54857:3771-3790 | MS.gene067271:intergenic | 30.0% |
| !!! | CTTTTTTCTACTTCTTGTTG+AGG | + | 54857:3681-3700 | MS.gene067271:intergenic | 30.0% |
| AAAGCCTCAAAATGGTAAAG+AGG | - | 54857:4103-4122 | MS.gene067271:CDS | 35.0% | |
| AAATGTTCCAGAGAAGGAAA+AGG | - | 54857:4403-4422 | MS.gene067271:intron | 35.0% | |
| ACTTGTGATGATTGTGAAGT+TGG | + | 54857:3654-3673 | MS.gene067271:intergenic | 35.0% | |
| ATAGATGAAAAGCCGCAAAA+TGG | - | 54857:4023-4042 | MS.gene067271:CDS | 35.0% | |
| CAATGACAATAACCCTCAAA+AGG | - | 54857:3893-3912 | MS.gene067271:CDS | 35.0% | |
| CAGGAAGATAAACATGAAGA+AGG | + | 54857:3203-3222 | MS.gene067271:intergenic | 35.0% | |
| CGACCATGTAAACAAGTTAA+AGG | + | 54857:3233-3252 | MS.gene067271:intergenic | 35.0% | |
| CTTCTTCATGTTTATCTTCC+TGG | - | 54857:3201-3220 | MS.gene067271:CDS | 35.0% | |
| CTTGAAGCCAAATTTGAACA+AGG | - | 54857:3600-3619 | MS.gene067271:CDS | 35.0% | |
| GAAAGAAAGGAACTCAAAGA+AGG | - | 54857:4286-4305 | MS.gene067271:intron | 35.0% | |
| GAGAGTTCCTTGTTCAAATT+TGG | + | 54857:3610-3629 | MS.gene067271:intergenic | 35.0% | |
| GGAAAACCAATATGACACAT+AGG | + | 54857:4600-4619 | MS.gene067271:intergenic | 35.0% | |
| GTTTCTTTCATCTCTGATAG+AGG | + | 54857:3168-3187 | MS.gene067271:intergenic | 35.0% | |
| TATTCCACCACCACAAAAAT+TGG | - | 54857:3758-3777 | MS.gene067271:CDS | 35.0% | |
| TGATTCTTGCTTGACTCATA+AGG | + | 54857:4338-4357 | MS.gene067271:intergenic | 35.0% | |
| TTGTGACAAGAATTGCAGAA+GGG | - | 54857:4501-4520 | MS.gene067271:intron | 35.0% | |
| TTTGTGACAAGAATTGCAGA+AGG | - | 54857:4500-4519 | MS.gene067271:intron | 35.0% | |
| ! | AATTCTTCTTGACCCTTTTG+AGG | + | 54857:3984-4003 | MS.gene067271:intergenic | 35.0% |
| ! | AATTCTTGTTGACCCTTTTG+AGG | + | 54857:3909-3928 | MS.gene067271:intergenic | 35.0% |
| ! | ACAAGTTAAAGGCATATACC+AGG | + | 54857:3222-3241 | MS.gene067271:intergenic | 35.0% |
| ! | ATTCTTGTTGACCCTTTTGA+GGG | + | 54857:3908-3927 | MS.gene067271:intergenic | 35.0% |
| ! | CTCCATTTGTTAACTGGAAT+CGG | + | 54857:3531-3550 | MS.gene067271:intergenic | 35.0% |
| ! | GATTCTTCCAATTTTTGTGG+TGG | + | 54857:3768-3787 | MS.gene067271:intergenic | 35.0% |
| ! | GGCTTTTCATCTATTGATGT+TGG | + | 54857:4017-4036 | MS.gene067271:intergenic | 35.0% |
| ! | TCAAATGAAGATTGAGTTGC+TGG | - | 54857:3470-3489 | MS.gene067271:CDS | 35.0% |
| ! | TCCAATACTTGACCATTTTG+CGG | + | 54857:4038-4057 | MS.gene067271:intergenic | 35.0% |
| !! | AATTTGCTGCTACTTCTTCT+CGG | - | 54857:4477-4496 | MS.gene067271:intron | 35.0% |
| !! | TCAATGCTTTGAGAAGTTTG+AGG | + | 54857:3816-3835 | MS.gene067271:intergenic | 35.0% |
| !! | TTTCTGGAACAGGATATGTT+TGG | + | 54857:3563-3582 | MS.gene067271:intergenic | 35.0% |
| !!! | TTGCTTTTTGGTTGTGTCTA+TGG | + | 54857:3949-3968 | MS.gene067271:intergenic | 35.0% |
| !!! | TTGTGTTTTGGTTCTCTCTA+TGG | + | 54857:4075-4094 | MS.gene067271:intergenic | 35.0% |
| !!! | TTTTCCTTCTCTGGAACATT+TGG | + | 54857:4404-4423 | MS.gene067271:intergenic | 35.0% |
| !!! | TTTTTGTGGTGGTGGAATAT+TGG | + | 54857:3757-3776 | MS.gene067271:intergenic | 35.0% |
| ACATGAAGAAGGTAAGCTTC+AGG | + | 54857:3192-3211 | MS.gene067271:intergenic | 40.0% | |
| AGAAAGGAACTCAAAGAAGG+AGG | - | 54857:4289-4308 | MS.gene067271:intron | 40.0% | |
| AGAATTCCTCCCAATCAAGA+AGG | - | 54857:4437-4456 | MS.gene067271:intron | 40.0% | |
| ATTGCAGAAGGGAAATTGAG+TGG | - | 54857:4512-4531 | MS.gene067271:intron | 40.0% | |
| CCATTCTTCCCTTCTTGATT+GGG | + | 54857:4449-4468 | MS.gene067271:intergenic | 40.0% | |
| CTGAAGATGCTGAGAAAGAA+AGG | - | 54857:4273-4292 | MS.gene067271:intron | 40.0% | |
| GAATTCCTCCCAATCAAGAA+GGG | - | 54857:4438-4457 | MS.gene067271:intron | 40.0% | |
| GTTCTAGTGATTGCTGCATT+TGG | - | 54857:4572-4591 | MS.gene067271:CDS | 40.0% | |
| TCCATTCTTCCCTTCTTGAT+TGG | + | 54857:4450-4469 | MS.gene067271:intergenic | 40.0% | |
| TCCTGTTCCAGAAAATAGTG+TGG | - | 54857:3569-3588 | MS.gene067271:CDS | 40.0% | |
| TTGGAGCCTATGTGTCATAT+TGG | - | 54857:4591-4610 | MS.gene067271:CDS | 40.0% | |
| TTGGTTGTGTCTATGGATGT+TGG | + | 54857:3942-3961 | MS.gene067271:intergenic | 40.0% | |
| ! | GACCGATTCCAGTTAACAAA+TGG | - | 54857:3526-3545 | MS.gene067271:CDS | 40.0% |
| ! | TCTTCCAATTTTTGTGGTGG+TGG | + | 54857:3765-3784 | MS.gene067271:intergenic | 40.0% |
| ! | TTCTCTGCCACACTATTTTC+TGG | + | 54857:3579-3598 | MS.gene067271:intergenic | 40.0% |
| ! | TTGGTTCTCTCTATGGATGT+TGG | + | 54857:4068-4087 | MS.gene067271:intergenic | 40.0% |
| !!! | GTGTCTATGGATGTTGGTTT+TGG | + | 54857:3936-3955 | MS.gene067271:intergenic | 40.0% |
| !!! | TTGTGTTTTGGTTCTCTCTG+TGG | + | 54857:4150-4169 | MS.gene067271:intergenic | 40.0% |
| AAAGGCTCAAAAGGGTGAAG+AGG | - | 54857:4178-4197 | MS.gene067271:CDS | 45.0% | |
| CCCAATCAAGAAGGGAAGAA+TGG | - | 54857:4446-4465 | MS.gene067271:intron | 45.0% | |
| GCCGCAAAATGGTCAAGTAT+TGG | - | 54857:4034-4053 | MS.gene067271:CDS | 45.0% | |
| GGACATGTCACGAAAGTTGA+GGG | - | 54857:3491-3510 | MS.gene067271:CDS | 45.0% | |
| TCACGAAAGTTGAGGGTTAC+AGG | - | 54857:3498-3517 | MS.gene067271:CDS | 45.0% | |
| TGCTCCAAATGTTCCAGAGA+AGG | - | 54857:4397-4416 | MS.gene067271:intron | 45.0% | |
| TGGACATGTCACGAAAGTTG+AGG | - | 54857:3490-3509 | MS.gene067271:CDS | 45.0% | |
| TTCTTCCCTTCTTGATTGGG+AGG | + | 54857:4446-4465 | MS.gene067271:intergenic | 45.0% | |
| ! | ACAATGACACAGTTGATGCG+AGG | + | 54857:3333-3352 | MS.gene067271:intergenic | 45.0% |
| ! | CAAGCTTCCTTTTCCTTCTC+TGG | + | 54857:4413-4432 | MS.gene067271:intergenic | 45.0% |
| ! | TTGGTTCTCTCTGTGGATGT+TGG | + | 54857:4143-4162 | MS.gene067271:intergenic | 45.0% |
| !!! | GCCACACTATTTTCTGGAAC+AGG | + | 54857:3573-3592 | MS.gene067271:intergenic | 45.0% |
| GAAAGTTGCGACCGGAAAGT+TGG | + | 54857:3117-3136 | MS.gene067271:intergenic | 50.0% | |
| GACGAACTGAAAGTTGCGAC+CGG | + | 54857:3125-3144 | MS.gene067271:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 54857 | gene | 3110 | 4625 | 3110 | ID=MS.gene067271 |
| 54857 | mRNA | 3110 | 4625 | 3110 | ID=MS.gene067271.t1;Parent=MS.gene067271 |
| 54857 | exon | 4513 | 4625 | 4513 | ID=MS.gene067271.t1.exon1;Parent=MS.gene067271.t1 |
| 54857 | CDS | 4513 | 4625 | 4513 | ID=cds.MS.gene067271.t1;Parent=MS.gene067271.t1 |
| 54857 | exon | 3110 | 4278 | 3110 | ID=MS.gene067271.t1.exon2;Parent=MS.gene067271.t1 |
| 54857 | CDS | 3110 | 4278 | 3110 | ID=cds.MS.gene067271.t1;Parent=MS.gene067271.t1 |
| Gene Sequence |
| Protein sequence |
