Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068453.t1 | KRG90810.1 | 43.7 | 135 | 42 | 2 | 8 | 138 | 8 | 112 | 4.90E-15 | 91.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068453.t1 | Q8GW29 | 54.8 | 62 | 23 | 1 | 111 | 167 | 52 | 113 | 6.5e-08 | 58.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068453.t1 | I1NFI4 | 43.7 | 135 | 42 | 2 | 8 | 138 | 8 | 112 | 3.5e-15 | 91.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene068453 | 0.817009 | 4.12E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene068453 | 0.800499 | 1.40E-48 | -1.69E-46 |
| MS.gene049853 | MS.gene068453 | 0.825841 | 3.76E-54 | -1.69E-46 |
| MS.gene051710 | MS.gene068453 | 0.808081 | 3.69E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene068453 | 0.817922 | 2.56E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene068453 | 0.829998 | 3.76E-55 | -1.69E-46 |
| MS.gene059276 | MS.gene068453 | 0.824541 | 7.63E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068453.t1 | MTR_1g111560 | 50.962 | 104 | 19 | 1 | 35 | 138 | 519 | 590 | 7.15e-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068453.t1 | AT5G06300 | 53.125 | 64 | 25 | 1 | 109 | 167 | 50 | 113 | 2.27e-11 | 60.5 |
| MS.gene068453.t1 | AT2G37210 | 50.704 | 71 | 30 | 1 | 109 | 174 | 55 | 125 | 5.92e-11 | 59.7 |
| MS.gene068453.t1 | AT2G28305 | 54.688 | 64 | 24 | 1 | 109 | 167 | 49 | 112 | 9.68e-11 | 57.8 |
Find 40 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGCCAGACCTTGAGAATT+AGG | 0.252881 | 6.3:-64822001 | MS.gene068453:CDS |
| TAATGGCAATGTTAAAGTTC+TGG | 0.312277 | 6.3:-64822597 | MS.gene068453:CDS |
| AGTGATAGCTTATTTGCTAT+TGG | 0.375797 | 6.3:-64822020 | MS.gene068453:CDS |
| TTTGGTGAGAGTGGTGATCT+TGG | 0.380198 | 6.3:+64822182 | None:intergenic |
| CCAAAGTTAAAAGACGTGTC+TGG | 0.393542 | 6.3:+64822108 | None:intergenic |
| TAATATCAAGTAAGCTTTGA+AGG | 0.430549 | 6.3:+64821103 | None:intergenic |
| AGTGGTGATCTTGGTGGTGA+TGG | 0.441411 | 6.3:+64822191 | None:intergenic |
| ATTGTTAGACATATCTATCA+AGG | 0.454524 | 6.3:-64822456 | MS.gene068453:CDS |
| CTATCCTAATTCTCAAGGTC+TGG | 0.456407 | 6.3:+64821997 | None:intergenic |
| AGTTCTGGAGGGTTCATCTC+AGG | 0.457693 | 6.3:-64822582 | MS.gene068453:intron |
| GATGGAAATGAGGAAGAAAA+TGG | 0.461482 | 6.3:-64822417 | MS.gene068453:CDS |
| CTGTGCAGTAGGTGAAGTTA+AGG | 0.464088 | 6.3:-64821729 | MS.gene068453:intron |
| CATACACAATTATTGAATTG+AGG | 0.466047 | 6.3:+64822060 | None:intergenic |
| GGTGAGAGTGGTGATCTTGG+TGG | 0.492549 | 6.3:+64822185 | None:intergenic |
| AAAAGACGTGTCTGGGAGTA+GGG | 0.501716 | 6.3:+64822116 | None:intergenic |
| TAAAAGACGTGTCTGGGAGT+AGG | 0.508502 | 6.3:+64822115 | None:intergenic |
| CAAAGTTAAAAGACGTGTCT+GGG | 0.514240 | 6.3:+64822109 | None:intergenic |
| AATATCAAGTAAGCTTTGAA+GGG | 0.520804 | 6.3:+64821104 | None:intergenic |
| AATTGCATGCTAACCTGGTA+AGG | 0.524671 | 6.3:+64821646 | None:intergenic |
| GCCATGGCAAGCAAAAGAAA+AGG | 0.530423 | 6.3:-64822156 | MS.gene068453:CDS |
| GGGATTGTCATTCTATGTGA+TGG | 0.541354 | 6.3:-64822435 | MS.gene068453:CDS |
| TTGTGTATGCAGTTAACCAT+GGG | 0.551851 | 6.3:-64822046 | MS.gene068453:CDS |
| AAAGGAATGCTGAGACGGCT+AGG | 0.559617 | 6.3:-64821688 | MS.gene068453:CDS |
| TATACTCAGGATAGTGAACC+AGG | 0.561334 | 6.3:-64822236 | MS.gene068453:intron |
| TGGCAATGTTAAAGTTCTGG+AGG | 0.563314 | 6.3:-64822594 | MS.gene068453:CDS |
| GGCAATGTTAAAGTTCTGGA+GGG | 0.569855 | 6.3:-64822593 | MS.gene068453:CDS |
| GTGATCTTGGTGGTGATGGT+TGG | 0.570360 | 6.3:+64822195 | None:intergenic |
| TGGACCTATCCTAATTCTCA+AGG | 0.574067 | 6.3:+64821992 | None:intergenic |
| GGTTGGTCGTCAAAGACTAA+AGG | 0.576564 | 6.3:+64822212 | None:intergenic |
| CTGTTGCTGATATGCATCAA+AGG | 0.579948 | 6.3:-64821706 | MS.gene068453:CDS |
| ATTGTGTATGCAGTTAACCA+TGG | 0.586949 | 6.3:-64822047 | MS.gene068453:CDS |
| GCATCAAAGGAATGCTGAGA+CGG | 0.592859 | 6.3:-64821693 | MS.gene068453:CDS |
| GAGAAAGTCAAGCTTGCAGT+TGG | 0.599354 | 6.3:-64822363 | MS.gene068453:intron |
| ATAAGCTATCACTGTCCCCA+TGG | 0.604954 | 6.3:+64822030 | None:intergenic |
| TTGTTAGACATATCTATCAA+GGG | 0.612263 | 6.3:-64822455 | MS.gene068453:CDS |
| CATTCTATGTGATGGAAATG+AGG | 0.622047 | 6.3:-64822427 | MS.gene068453:CDS |
| CAGACCTTGAGAATTAGGAT+AGG | 0.646009 | 6.3:-64821996 | MS.gene068453:intron |
| TCGTCAAAGACTAAAGGACC+TGG | 0.651924 | 6.3:+64822218 | None:intergenic |
| GGAAATGAGGAAGAAAATGG+TGG | 0.688033 | 6.3:-64822414 | MS.gene068453:CDS |
| TGTGTATGCAGTTAACCATG+GGG | 0.736748 | 6.3:-64822045 | MS.gene068453:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAAAAATATGAAAATGT+CGG | + | chr6.3:64822172-64822191 | None:intergenic | 10.0% |
| !! | TTTAAAAATGAAAGATAAAA+AGG | + | chr6.3:64822310-64822329 | None:intergenic | 10.0% |
| !!! | ATATTTTTTTATTGTTTACT+TGG | - | chr6.3:64822180-64822199 | MS.gene068453:CDS | 10.0% |
| !! | ATTTCAAAGATACAAATTCA+TGG | + | chr6.3:64821868-64821887 | None:intergenic | 20.0% |
| !! | TCCAATGTTTATGATTAATA+GGG | - | chr6.3:64822568-64822587 | MS.gene068453:intron | 20.0% |
| !! | TTCCAATGTTTATGATTAAT+AGG | - | chr6.3:64822567-64822586 | MS.gene068453:intron | 20.0% |
| !!! | AATTTGTATCTTTGAAATTG+AGG | - | chr6.3:64821870-64821889 | MS.gene068453:intron | 20.0% |
| !!! | TGAAACAATTGTTTTTTCAA+AGG | + | chr6.3:64822149-64822168 | None:intergenic | 20.0% |
| !!! | TTGTTTTTGTTGATTTGATT+TGG | - | chr6.3:64822345-64822364 | MS.gene068453:intron | 20.0% |
| !!! | TTTTAAAACAACATGATTCA+AGG | + | chr6.3:64822465-64822484 | None:intergenic | 20.0% |
| ! | ATTGTTAGACATATCTATCA+AGG | - | chr6.3:64821247-64821266 | MS.gene068453:intron | 25.0% |
| ! | CATACAAACAAAAATCCATT+CGG | + | chr6.3:64821161-64821180 | None:intergenic | 25.0% |
| ! | CATACACAATTATTGAATTG+AGG | + | chr6.3:64821646-64821665 | None:intergenic | 25.0% |
| ! | TCAAAGTTATTCATTACTTG+TGG | + | chr6.3:64822380-64822399 | None:intergenic | 25.0% |
| ! | TCCCTATTAATCATAAACAT+TGG | + | chr6.3:64822572-64822591 | None:intergenic | 25.0% |
| ! | TTGTTAGACATATCTATCAA+GGG | - | chr6.3:64821248-64821267 | MS.gene068453:intron | 25.0% |
| !!! | AATATTTTGTGTCATTTTGC+AGG | - | chr6.3:64821213-64821232 | MS.gene068453:intron | 25.0% |
| !!! | ATATTTTGTGTCATTTTGCA+GGG | - | chr6.3:64821214-64821233 | MS.gene068453:intron | 25.0% |
| AAAGAGCAGTCAGTATAAAT+AGG | + | chr6.3:64821937-64821956 | None:intergenic | 30.0% | |
| AAGAGCAGTCAGTATAAATA+GGG | + | chr6.3:64821936-64821955 | None:intergenic | 30.0% | |
| AGGAATCAAACAATGAGATA+TGG | + | chr6.3:64822129-64822148 | None:intergenic | 30.0% | |
| ATGAATAACTTTGACTAGTG+TGG | - | chr6.3:64822386-64822405 | MS.gene068453:CDS | 30.0% | |
| CAAAAATAAGCACAAGTTCT+CGG | + | chr6.3:64821427-64821446 | None:intergenic | 30.0% | |
| TAATTCCTAAACAACTGTCA+TGG | - | chr6.3:64822530-64822549 | MS.gene068453:intron | 30.0% | |
| TTACTCATTGATCTATACTC+AGG | - | chr6.3:64821454-64821473 | MS.gene068453:intron | 30.0% | |
| ! | AAATGTTGTTGTGTTTTGTG+GGG | - | chr6.3:64822267-64822286 | MS.gene068453:intron | 30.0% |
| ! | CAAATGTTGTTGTGTTTTGT+GGG | - | chr6.3:64822266-64822285 | MS.gene068453:intron | 30.0% |
| !! | AGTGATAGCTTATTTGCTAT+TGG | - | chr6.3:64821683-64821702 | MS.gene068453:CDS | 30.0% |
| AATTAGGATAGGTCCATTGA+TGG | - | chr6.3:64821718-64821737 | MS.gene068453:CDS | 35.0% | |
| ATCCATTCGGAAATAAGCAT+CGG | + | chr6.3:64821148-64821167 | None:intergenic | 35.0% | |
| ATTAGGATAGGTCCATTGAT+GGG | - | chr6.3:64821719-64821738 | MS.gene068453:CDS | 35.0% | |
| ATTGTGTATGCAGTTAACCA+TGG | - | chr6.3:64821656-64821675 | MS.gene068453:intron | 35.0% | |
| CAAAGTTAAAAGACGTGTCT+GGG | + | chr6.3:64821597-64821616 | None:intergenic | 35.0% | |
| CCACAAAACACAACAACATT+TGG | + | chr6.3:64822268-64822287 | None:intergenic | 35.0% | |
| CCATTCATTGAGGAAAACAA+AGG | - | chr6.3:64822410-64822429 | MS.gene068453:CDS | 35.0% | |
| GATGGAAATGAGGAAGAAAA+TGG | - | chr6.3:64821286-64821305 | MS.gene068453:intron | 35.0% | |
| GATTGAAGCAATGAGAAAAG+AGG | + | chr6.3:64822507-64822526 | None:intergenic | 35.0% | |
| TTGTGTATGCAGTTAACCAT+GGG | - | chr6.3:64821657-64821676 | MS.gene068453:intron | 35.0% | |
| ! | CATTCTATGTGATGGAAATG+AGG | - | chr6.3:64821276-64821295 | MS.gene068453:intron | 35.0% |
| ! | CCAAATGTTGTTGTGTTTTG+TGG | - | chr6.3:64822265-64822284 | MS.gene068453:intron | 35.0% |
| !!! | CCTTTGTTTTCCTCAATGAA+TGG | + | chr6.3:64822413-64822432 | None:intergenic | 35.0% |
| !!! | TGGTTGCTTTTTAGACAGAA+TGG | - | chr6.3:64822200-64822219 | MS.gene068453:CDS | 35.0% |
| AATTGCATGCTAACCTGGTA+AGG | + | chr6.3:64822060-64822079 | None:intergenic | 40.0% | |
| ACACAAATTGCATGCTAACC+TGG | + | chr6.3:64822065-64822084 | None:intergenic | 40.0% | |
| ATCTTATCTAGTACCCTCCT+TGG | - | chr6.3:64821392-64821411 | MS.gene068453:intron | 40.0% | |
| CAAGACCAGCATAAACATAC+CGG | + | chr6.3:64821802-64821821 | None:intergenic | 40.0% | |
| CAGACCTTGAGAATTAGGAT+AGG | - | chr6.3:64821707-64821726 | MS.gene068453:CDS | 40.0% | |
| CCAAAGTTAAAAGACGTGTC+TGG | + | chr6.3:64821598-64821617 | None:intergenic | 40.0% | |
| CTATCCTAATTCTCAAGGTC+TGG | + | chr6.3:64821709-64821728 | None:intergenic | 40.0% | |
| CTGTTGCTGATATGCATCAA+AGG | - | chr6.3:64821997-64822016 | MS.gene068453:CDS | 40.0% | |
| GAAAGCCATGACAGTTGTTT+AGG | + | chr6.3:64822538-64822557 | None:intergenic | 40.0% | |
| GGAAATGAGGAAGAAAATGG+TGG | - | chr6.3:64821289-64821308 | MS.gene068453:intron | 40.0% | |
| GGGATTGTCATTCTATGTGA+TGG | - | chr6.3:64821268-64821287 | MS.gene068453:intron | 40.0% | |
| TATACTCAGGATAGTGAACC+AGG | - | chr6.3:64821467-64821486 | MS.gene068453:intron | 40.0% | |
| TCTCAGCAAAAGCTTCTTAC+CGG | - | chr6.3:64821780-64821799 | MS.gene068453:intron | 40.0% | |
| TCTTACCGGTATGTTTATGC+TGG | - | chr6.3:64821794-64821813 | MS.gene068453:intron | 40.0% | |
| TGGACCTATCCTAATTCTCA+AGG | + | chr6.3:64821714-64821733 | None:intergenic | 40.0% | |
| TGTGTATGCAGTTAACCATG+GGG | - | chr6.3:64821658-64821677 | MS.gene068453:intron | 40.0% | |
| TTGAGGCAGACTTGTATTAC+TGG | - | chr6.3:64821887-64821906 | MS.gene068453:intron | 40.0% | |
| ! | GATGCTTTTATTGCCTTACC+AGG | - | chr6.3:64822044-64822063 | MS.gene068453:CDS | 40.0% |
| ! | TCCTTTTCTTTTGCTTGCCA+TGG | + | chr6.3:64821551-64821570 | None:intergenic | 40.0% |
| ! | TGGCAATGTTAAAGTTCTGG+AGG | - | chr6.3:64821109-64821128 | MS.gene068453:CDS | 40.0% |
| !! | GGCAATGTTAAAGTTCTGGA+GGG | - | chr6.3:64821110-64821129 | MS.gene068453:CDS | 40.0% |
| !! | GTCATTTTGCAGGGTAAACA+AGG | - | chr6.3:64821223-64821242 | MS.gene068453:intron | 40.0% |
| !!! | CCAGACACGTCTTTTAACTT+TGG | - | chr6.3:64821595-64821614 | MS.gene068453:intron | 40.0% |
| !!! | TTTGCTTGCCATGGCTTTTT+TGG | + | chr6.3:64821542-64821561 | None:intergenic | 40.0% |
| AAAAGACGTGTCTGGGAGTA+GGG | + | chr6.3:64821590-64821609 | None:intergenic | 45.0% | |
| ATAAGCTATCACTGTCCCCA+TGG | + | chr6.3:64821676-64821695 | None:intergenic | 45.0% | |
| CACTCTCACCAAAAAAGCCA+TGG | - | chr6.3:64821531-64821550 | MS.gene068453:intron | 45.0% | |
| CATCATTCGTACTGTGCAGT+AGG | - | chr6.3:64821963-64821982 | MS.gene068453:intron | 45.0% | |
| CTAGTGTGGTCCATTCATTG+AGG | - | chr6.3:64822400-64822419 | MS.gene068453:CDS | 45.0% | |
| CTGTGCAGTAGGTGAAGTTA+AGG | - | chr6.3:64821974-64821993 | MS.gene068453:intron | 45.0% | |
| GAGAAAGTCAAGCTTGCAGT+TGG | - | chr6.3:64821340-64821359 | MS.gene068453:intron | 45.0% | |
| GCATCAAAGGAATGCTGAGA+CGG | - | chr6.3:64822010-64822029 | MS.gene068453:CDS | 45.0% | |
| GCCATGGCAAGCAAAAGAAA+AGG | - | chr6.3:64821547-64821566 | MS.gene068453:intron | 45.0% | |
| GTCCGATGCTTATTTCCGAA+TGG | - | chr6.3:64821143-64821162 | MS.gene068453:intron | 45.0% | |
| TAAAAGACGTGTCTGGGAGT+AGG | + | chr6.3:64821591-64821610 | None:intergenic | 45.0% | |
| TCGTCAAAGACTAAAGGACC+TGG | + | chr6.3:64821488-64821507 | None:intergenic | 45.0% | |
| TTGGCCAGACCTTGAGAATT+AGG | - | chr6.3:64821702-64821721 | MS.gene068453:CDS | 45.0% | |
| TTTGGTGAGAGTGGTGATCT+TGG | + | chr6.3:64821524-64821543 | None:intergenic | 45.0% | |
| ! | CGGTATGTTTATGCTGGTCT+TGG | - | chr6.3:64821800-64821819 | MS.gene068453:intron | 45.0% |
| ! | GGTTGGTCGTCAAAGACTAA+AGG | + | chr6.3:64821494-64821513 | None:intergenic | 45.0% |
| ! | TGTTTTCAGCGCAACTGGAA+AGG | - | chr6.3:64821363-64821382 | MS.gene068453:intron | 45.0% |
| !!! | CATGGCTTTTTTGGTGAGAG+TGG | + | chr6.3:64821533-64821552 | None:intergenic | 45.0% |
| !!! | AAATATTTTAAAAAATATCA+AGG | + | chr6.3:64821200-64821219 | None:intergenic | 5.0% |
| !!! | AATATTTTAAAAAATATCAA+GGG | + | chr6.3:64821199-64821218 | None:intergenic | 5.0% |
| AAAGGAATGCTGAGACGGCT+AGG | - | chr6.3:64822015-64822034 | MS.gene068453:CDS | 50.0% | |
| TCGGTGTGTGTAACCAAGGA+GGG | + | chr6.3:64821408-64821427 | None:intergenic | 50.0% | |
| ! | GTTCTCGGTGTGTGTAACCA+AGG | + | chr6.3:64821412-64821431 | None:intergenic | 50.0% |
| !! | AGTGGTGATCTTGGTGGTGA+TGG | + | chr6.3:64821515-64821534 | None:intergenic | 50.0% |
| !! | AGTTCTGGAGGGTTCATCTC+AGG | - | chr6.3:64821121-64821140 | MS.gene068453:intron | 50.0% |
| !! | GTGATCTTGGTGGTGATGGT+TGG | + | chr6.3:64821511-64821530 | None:intergenic | 50.0% |
| !! | GTTGGTGTTTTCAGCGCAAC+TGG | - | chr6.3:64821358-64821377 | MS.gene068453:intron | 50.0% |
| GACGTATGCCAGCCCATCAA+TGG | + | chr6.3:64821734-64821753 | None:intergenic | 55.0% | |
| GGTGAGAGTGGTGATCTTGG+TGG | + | chr6.3:64821521-64821540 | None:intergenic | 55.0% | |
| ! | CTCGGTGTGTGTAACCAAGG+AGG | + | chr6.3:64821409-64821428 | None:intergenic | 55.0% |
| ! | GGATAGGTCCATTGATGGGC+TGG | - | chr6.3:64821723-64821742 | MS.gene068453:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 64821108 | 64822617 | 64821108 | ID=MS.gene068453 |
| chr6.3 | mRNA | 64821108 | 64822617 | 64821108 | ID=MS.gene068453.t1;Parent=MS.gene068453 |
| chr6.3 | exon | 64822583 | 64822617 | 64822583 | ID=MS.gene068453.t1.exon1;Parent=MS.gene068453.t1 |
| chr6.3 | CDS | 64822583 | 64822617 | 64822583 | ID=cds.MS.gene068453.t1;Parent=MS.gene068453.t1 |
| chr6.3 | exon | 64822364 | 64822490 | 64822364 | ID=MS.gene068453.t1.exon2;Parent=MS.gene068453.t1 |
| chr6.3 | CDS | 64822364 | 64822490 | 64822364 | ID=cds.MS.gene068453.t1;Parent=MS.gene068453.t1 |
| chr6.3 | exon | 64821997 | 64822249 | 64821997 | ID=MS.gene068453.t1.exon3;Parent=MS.gene068453.t1 |
| chr6.3 | CDS | 64821997 | 64822249 | 64821997 | ID=cds.MS.gene068453.t1;Parent=MS.gene068453.t1 |
| chr6.3 | exon | 64821660 | 64821743 | 64821660 | ID=MS.gene068453.t1.exon4;Parent=MS.gene068453.t1 |
| chr6.3 | CDS | 64821660 | 64821743 | 64821660 | ID=cds.MS.gene068453.t1;Parent=MS.gene068453.t1 |
| chr6.3 | exon | 64821108 | 64821136 | 64821108 | ID=MS.gene068453.t1.exon5;Parent=MS.gene068453.t1 |
| chr6.3 | CDS | 64821108 | 64821136 | 64821108 | ID=cds.MS.gene068453.t1;Parent=MS.gene068453.t1 |
| Gene Sequence |
| Protein sequence |
