Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06871.t1 | XP_003602304.1 | 98.8 | 162 | 2 | 0 | 1 | 162 | 1 | 162 | 7.50E-87 | 329.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06871.t1 | G7J857 | 98.8 | 162 | 2 | 0 | 1 | 162 | 1 | 162 | 5.4e-87 | 329.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene06871 | 0.812364 | 4.40E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene06871 | 0.800016 | 1.76E-48 | -1.69E-46 |
| MS.gene052333 | MS.gene06871 | 0.812988 | 3.21E-51 | -1.69E-46 |
| MS.gene052464 | MS.gene06871 | 0.829189 | 5.92E-55 | -1.69E-46 |
| MS.gene053584 | MS.gene06871 | 0.815001 | 1.16E-51 | -1.69E-46 |
| MS.gene054093 | MS.gene06871 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene06871 | 0.830014 | 3.73E-55 | -1.69E-46 |
| MS.gene055701 | MS.gene06871 | 0.830743 | 2.47E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene06871 | 0.801238 | 9.90E-49 | -1.69E-46 |
| MS.gene057194 | MS.gene06871 | 0.958749 | 1.09E-116 | -1.69E-46 |
| MS.gene05761 | MS.gene06871 | 0.836002 | 1.21E-56 | -1.69E-46 |
| MS.gene060011 | MS.gene06871 | 0.814215 | 1.73E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06871.t1 | MTR_3g092020 | 98.765 | 162 | 2 | 0 | 1 | 162 | 1 | 162 | 6.52e-119 | 332 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCCCATCTCTCCTTAAAA+AGG | 0.245866 | 3.1:-74056197 | None:intergenic |
| CAAAATGGAAACCTTACCTT+TGG | 0.392712 | 3.1:+74055038 | None:intergenic |
| CTCCTCTTGGTTCAAAATCA+TGG | 0.393681 | 3.1:+74056241 | MS.gene06871:CDS |
| GAGATCATGTCGAGGAATCT+TGG | 0.402090 | 3.1:+74056895 | MS.gene06871:CDS |
| TGGCCTCGGACAATGCTCTA+TGG | 0.432233 | 3.1:+74056169 | MS.gene06871:CDS |
| TTATATCACACGGCTGCGTT+TGG | 0.432650 | 3.1:-74056966 | None:intergenic |
| CATACAGATTTCCAAAGGTA+AGG | 0.450513 | 3.1:-74055049 | None:intergenic |
| GGTGAGGTACAACGATATCT+AGG | 0.477811 | 3.1:+74056788 | MS.gene06871:CDS |
| AGTTCAGAAACAGACTTCAC+TGG | 0.488792 | 3.1:+74056851 | MS.gene06871:CDS |
| GAAGTGGAAGCTCATGGCCT+CGG | 0.508465 | 3.1:+74056155 | MS.gene06871:CDS |
| GGTTTGAAGATTGTTAGAGA+AGG | 0.530829 | 3.1:+74056740 | MS.gene06871:CDS |
| TTGTTGAGAAACAGCAAGAT+GGG | 0.537051 | 3.1:-74055097 | None:intergenic |
| ATCATGGAAAGAAGTGTATG+AGG | 0.545115 | 3.1:+74056257 | MS.gene06871:CDS |
| CATCTTGCTGTTTCTCAACA+AGG | 0.545411 | 3.1:+74055099 | MS.gene06871:CDS |
| CAAGATCGATGCGATCGAGT+TGG | 0.561970 | 3.1:+74056282 | MS.gene06871:CDS |
| CTGTAGGAAGTGGAAGCTCA+TGG | 0.592096 | 3.1:+74056149 | MS.gene06871:CDS |
| CTTGTTGAGAAACAGCAAGA+TGG | 0.595023 | 3.1:-74055098 | None:intergenic |
| ATCTTCATACAGATTTCCAA+AGG | 0.614774 | 3.1:-74055054 | None:intergenic |
| GAGAAACAGCAAGATGGGTG+TGG | 0.636024 | 3.1:-74055092 | None:intergenic |
| GATATCGTTGTACCTCACCT+TGG | 0.640965 | 3.1:-74056784 | None:intergenic |
| CTATCTTGTTCACCAAGGTG+AGG | 0.666547 | 3.1:+74056772 | MS.gene06871:CDS |
| GTAGAGATGGTTATATCACA+CGG | 0.677630 | 3.1:-74056976 | None:intergenic |
| TGACCATAGAGCATTGTCCG+AGG | 0.683112 | 3.1:-74056172 | None:intergenic |
| GCTGAAGAGAGATCATGTCG+AGG | 0.706201 | 3.1:+74056887 | MS.gene06871:CDS |
| GACTACTATCTTGTTCACCA+AGG | 0.711703 | 3.1:+74056767 | MS.gene06871:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTAATTAATAATAATTT+TGG | + | chr3.1:74056470-74056489 | MS.gene06871:intron | 0.0% |
| !!! | AAAAAATTAACAAATTGTTT+TGG | - | chr3.1:74055936-74055955 | None:intergenic | 10.0% |
| !!! | TTTTAGAAGAAAAAAAATAT+GGG | - | chr3.1:74056499-74056518 | None:intergenic | 10.0% |
| !! | AATTGAATCAATCATAATAT+TGG | + | chr3.1:74055835-74055854 | MS.gene06871:intron | 15.0% |
| !! | AATTGTATAACAAAATTTGT+TGG | - | chr3.1:74055521-74055540 | None:intergenic | 15.0% |
| !!! | AACAATAAAGATTGAATTTT+AGG | - | chr3.1:74055729-74055748 | None:intergenic | 15.0% |
| !!! | ACAAAATTCTTTTTTTCATT+GGG | + | chr3.1:74056918-74056937 | MS.gene06871:CDS | 15.0% |
| !!! | ATAATTTGTTATGTTGAATA+TGG | - | chr3.1:74056604-74056623 | None:intergenic | 15.0% |
| !!! | GTTTTAGAAGAAAAAAAATA+TGG | - | chr3.1:74056500-74056519 | None:intergenic | 15.0% |
| !!! | TTTTAATTCTTCACAAAATA+GGG | + | chr3.1:74056718-74056737 | MS.gene06871:intron | 15.0% |
| !!! | TTTTTAATTCTTCACAAAAT+AGG | + | chr3.1:74056717-74056736 | MS.gene06871:intron | 15.0% |
| !! | AAAACAATTTGTCAAATTCA+AGG | - | chr3.1:74056124-74056143 | None:intergenic | 20.0% |
| !! | ATCAATCATAATATTGGATT+AGG | + | chr3.1:74055841-74055860 | MS.gene06871:intron | 20.0% |
| !! | TTATTGCTTCAAATTAAACT+AGG | - | chr3.1:74055565-74055584 | None:intergenic | 20.0% |
| !! | TTGACTGTAATGATATAAAA+AGG | - | chr3.1:74055817-74055836 | None:intergenic | 20.0% |
| !! | TTTAATTCTTCACAAAATAG+GGG | + | chr3.1:74056719-74056738 | MS.gene06871:intron | 20.0% |
| !!! | ATTTGAAATTCATAAGTCAA+CGG | - | chr3.1:74055221-74055240 | None:intergenic | 20.0% |
| !!! | CAAAATTCTTTTTTTCATTG+GGG | + | chr3.1:74056919-74056938 | MS.gene06871:CDS | 20.0% |
| !!! | CTGAGTTTATTACATTATTT+TGG | + | chr3.1:74056093-74056112 | MS.gene06871:intron | 20.0% |
| !!! | GACAAAATTCTTTTTTTCAT+TGG | + | chr3.1:74056917-74056936 | MS.gene06871:CDS | 20.0% |
| !!! | TCAATCTTTATTGTTTTGAT+CGG | + | chr3.1:74055734-74055753 | MS.gene06871:intron | 20.0% |
| !!! | TTGTCAAATATCTTTTTAGA+GGG | - | chr3.1:74056043-74056062 | None:intergenic | 20.0% |
| ! | AAAATCTCCTTCAATAATCA+TGG | - | chr3.1:74056390-74056409 | None:intergenic | 25.0% |
| ! | AACCTATGTGTAACATTAAA+AGG | - | chr3.1:74055967-74055986 | None:intergenic | 25.0% |
| ! | AATAACAATATTTCAACCGA+TGG | - | chr3.1:74055256-74055275 | None:intergenic | 25.0% |
| ! | ATCTAGGTTCAAGAAAAAAT+CGG | + | chr3.1:74056804-74056823 | MS.gene06871:CDS | 25.0% |
| ! | CGAGAATATAAAAATGAAGA+TGG | - | chr3.1:74055155-74055174 | None:intergenic | 25.0% |
| ! | TGTTGGATATCATATGATAT+AGG | - | chr3.1:74055504-74055523 | None:intergenic | 25.0% |
| ! | TTAAATGCCATGATTATTGA+AGG | + | chr3.1:74056380-74056399 | MS.gene06871:intron | 25.0% |
| ! | TTATTAGATCTGAATCTGTA+TGG | + | chr3.1:74055271-74055290 | MS.gene06871:intron | 25.0% |
| !! | ACTGTGATTTTATGATACAT+TGG | + | chr3.1:74055770-74055789 | MS.gene06871:intron | 25.0% |
| !! | CTTGTCAAATATCTTTTTAG+AGG | - | chr3.1:74056044-74056063 | None:intergenic | 25.0% |
| !! | TTCCTTTTAATGTTACACAT+AGG | + | chr3.1:74055962-74055981 | MS.gene06871:intron | 25.0% |
| !! | TTTTTGCACACTTATAACAT+TGG | + | chr3.1:74056534-74056553 | MS.gene06871:intron | 25.0% |
| !!! | TTTTGTTTTCTTCGAAGAAA+TGG | + | chr3.1:74055444-74055463 | MS.gene06871:intron | 25.0% |
| AAATATGAATCAAGTCCATG+TGG | - | chr3.1:74055693-74055712 | None:intergenic | 30.0% | |
| ATCTTCATACAGATTTCCAA+AGG | - | chr3.1:74055057-74055076 | None:intergenic | 30.0% | |
| TCTTTATCAGAAAACAGTGT+TGG | + | chr3.1:74055375-74055394 | MS.gene06871:intron | 30.0% | |
| TTATTGAACTCTGACACAAA+GGG | + | chr3.1:74056687-74056706 | MS.gene06871:intron | 30.0% | |
| ! | TCTATGGTCAAACCTTTTTA+AGG | + | chr3.1:74056185-74056204 | MS.gene06871:CDS | 30.0% |
| !! | AAACCTTTTTAAGGAGAGAT+GGG | + | chr3.1:74056194-74056213 | MS.gene06871:CDS | 30.0% |
| !! | CAAATTGTTTTACAGTCTGT+AGG | + | chr3.1:74056133-74056152 | MS.gene06871:intron | 30.0% |
| !!! | TCTTTTTTTCATTGGGGATT+TGG | + | chr3.1:74056925-74056944 | MS.gene06871:CDS | 30.0% |
| AAATCAGTTGCATGTTCCAT+CGG | + | chr3.1:74055237-74055256 | MS.gene06871:intron | 35.0% | |
| ATAATTGCAAGCGTGTGATT+AGG | - | chr3.1:74056424-74056443 | None:intergenic | 35.0% | |
| ATAGGTTAAAACCGACGATT+TGG | + | chr3.1:74055980-74055999 | MS.gene06871:intron | 35.0% | |
| ATTATTCTTGTCCCTTGAGA+TGG | + | chr3.1:74056439-74056458 | MS.gene06871:intron | 35.0% | |
| ATTTGGCAACTGAGTGTTAA+CGG | + | chr3.1:74056004-74056023 | MS.gene06871:intron | 35.0% | |
| CACACTTATAACATTGGTGT+TGG | + | chr3.1:74056540-74056559 | MS.gene06871:intron | 35.0% | |
| CATACAGATTTCCAAAGGTA+AGG | - | chr3.1:74055052-74055071 | None:intergenic | 35.0% | |
| CTAAAGCAAATCCAAGTAAG+AGG | - | chr3.1:74056569-74056588 | None:intergenic | 35.0% | |
| CTTATTGAACTCTGACACAA+AGG | + | chr3.1:74056686-74056705 | MS.gene06871:intron | 35.0% | |
| GGATTAACAATATGTGACAG+AGG | - | chr3.1:74055483-74055502 | None:intergenic | 35.0% | |
| GGTCAAATTCCAGATCATAA+TGG | - | chr3.1:74055662-74055681 | None:intergenic | 35.0% | |
| GGTTTGAAGATTGTTAGAGA+AGG | + | chr3.1:74056740-74056759 | MS.gene06871:CDS | 35.0% | |
| TTGTTGAGAAACAGCAAGAT+GGG | - | chr3.1:74055100-74055119 | None:intergenic | 35.0% | |
| TTTGACCAAAATTTGCCACA+TGG | + | chr3.1:74055675-74055694 | MS.gene06871:intron | 35.0% | |
| ! | ATAATTTTCACGCCATCTCA+AGG | - | chr3.1:74056454-74056473 | None:intergenic | 35.0% |
| ! | ATCATGGAAAGAAGTGTATG+AGG | + | chr3.1:74056257-74056276 | MS.gene06871:CDS | 35.0% |
| ! | CTGTGTTTCTTTGAAAAGCA+TGG | - | chr3.1:74055628-74055647 | None:intergenic | 35.0% |
| ! | TAATTTTCACGCCATCTCAA+GGG | - | chr3.1:74056453-74056472 | None:intergenic | 35.0% |
| ! | TTATCAGAAAACAGTGTTGG+TGG | + | chr3.1:74055378-74055397 | MS.gene06871:intron | 35.0% |
| ! | TTCCATGATTTTGAACCAAG+AGG | - | chr3.1:74056246-74056265 | None:intergenic | 35.0% |
| !! | AAAACCGACGATTTGGTATT+TGG | + | chr3.1:74055987-74056006 | MS.gene06871:intron | 35.0% |
| !! | AACCTTTTTAAGGAGAGATG+GGG | + | chr3.1:74056195-74056214 | MS.gene06871:CDS | 35.0% |
| !! | CAAACCTTTTTAAGGAGAGA+TGG | + | chr3.1:74056193-74056212 | MS.gene06871:CDS | 35.0% |
| AAAAATCGGCGACAAGTATC+TGG | + | chr3.1:74056818-74056837 | MS.gene06871:CDS | 40.0% | |
| AAACACACCGCTATGTTTCT+TGG | + | chr3.1:74055584-74055603 | MS.gene06871:intron | 40.0% | |
| AACACACCGCTATGTTTCTT+GGG | + | chr3.1:74055585-74055604 | MS.gene06871:intron | 40.0% | |
| AGTTCAGAAACAGACTTCAC+TGG | + | chr3.1:74056851-74056870 | MS.gene06871:CDS | 40.0% | |
| CTCCTCTTGGTTCAAAATCA+TGG | + | chr3.1:74056241-74056260 | MS.gene06871:CDS | 40.0% | |
| CTTGTTGAGAAACAGCAAGA+TGG | - | chr3.1:74055101-74055120 | None:intergenic | 40.0% | |
| GACTACTATCTTGTTCACCA+AGG | + | chr3.1:74056767-74056786 | MS.gene06871:CDS | 40.0% | |
| GCAAATCAGCAAGACATATG+CGG | - | chr3.1:74055914-74055933 | None:intergenic | 40.0% | |
| GGTTCACAGAACACAGTTAT+TGG | - | chr3.1:74055544-74055563 | None:intergenic | 40.0% | |
| GTTGCCAAATACCAAATCGT+CGG | - | chr3.1:74055994-74056013 | None:intergenic | 40.0% | |
| TTACTCTGCTGAGAGTAGTA+CGG | + | chr3.1:74056322-74056341 | MS.gene06871:intron | 40.0% | |
| ! | CAAGTCCATGTGGCAAATTT+TGG | - | chr3.1:74055683-74055702 | None:intergenic | 40.0% |
| ! | GTGTTGGTGGAATTGAAAAC+AGG | + | chr3.1:74055391-74055410 | MS.gene06871:intron | 40.0% |
| ! | GTTTTACAGTCTGTAGGAAG+TGG | + | chr3.1:74056139-74056158 | MS.gene06871:intron | 40.0% |
| !! | CATCTTGCTGTTTCTCAACA+AGG | + | chr3.1:74055099-74055118 | MS.gene06871:CDS | 40.0% |
| CCTCAGCTTCCATTATGATC+TGG | + | chr3.1:74055650-74055669 | MS.gene06871:intron | 45.0% | |
| CTATCTTGTTCACCAAGGTG+AGG | + | chr3.1:74056772-74056791 | MS.gene06871:CDS | 45.0% | |
| CTCCCCATCTCTCCTTAAAA+AGG | - | chr3.1:74056200-74056219 | None:intergenic | 45.0% | |
| GAGATCATGTCGAGGAATCT+TGG | + | chr3.1:74056895-74056914 | MS.gene06871:CDS | 45.0% | |
| GATATCGTTGTACCTCACCT+TGG | - | chr3.1:74056787-74056806 | None:intergenic | 45.0% | |
| GCATGGTGAAAGTTAGAGTC+TGG | - | chr3.1:74055611-74055630 | None:intergenic | 45.0% | |
| GGTGAGGTACAACGATATCT+AGG | + | chr3.1:74056788-74056807 | MS.gene06871:CDS | 45.0% | |
| TTATATCACACGGCTGCGTT+TGG | - | chr3.1:74056969-74056988 | None:intergenic | 45.0% | |
| ! | CCAGATCATAATGGAAGCTG+AGG | - | chr3.1:74055653-74055672 | None:intergenic | 45.0% |
| !! | CTTTTTAAGGAGAGATGGGG+AGG | + | chr3.1:74056198-74056217 | MS.gene06871:CDS | 45.0% |
| !!! | GCTGCTTTTTATGCTCCTCT+TGG | + | chr3.1:74056228-74056247 | MS.gene06871:CDS | 45.0% |
| CAAGATCGATGCGATCGAGT+TGG | + | chr3.1:74056282-74056301 | MS.gene06871:CDS | 50.0% | |
| CTGTAGGAAGTGGAAGCTCA+TGG | + | chr3.1:74056149-74056168 | MS.gene06871:CDS | 50.0% | |
| GAGAAACAGCAAGATGGGTG+TGG | - | chr3.1:74055095-74055114 | None:intergenic | 50.0% | |
| GCTGAAGAGAGATCATGTCG+AGG | + | chr3.1:74056887-74056906 | MS.gene06871:CDS | 50.0% | |
| GTCTGGCCCAAGAAACATAG+CGG | - | chr3.1:74055594-74055613 | None:intergenic | 50.0% | |
| !! | GGTGTTGGCTTCCTCTTACT+TGG | + | chr3.1:74056555-74056574 | MS.gene06871:intron | 50.0% |
| !! | TGACCATAGAGCATTGTCCG+AGG | - | chr3.1:74056175-74056194 | None:intergenic | 50.0% |
| GAAGTGGAAGCTCATGGCCT+CGG | + | chr3.1:74056155-74056174 | MS.gene06871:CDS | 55.0% | |
| TGGCCTCGGACAATGCTCTA+TGG | + | chr3.1:74056169-74056188 | MS.gene06871:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 74055042 | 74056988 | 74055042 | ID=MS.gene06871 |
| chr3.1 | mRNA | 74055042 | 74056988 | 74055042 | ID=MS.gene06871.t1;Parent=MS.gene06871 |
| chr3.1 | exon | 74055042 | 74055120 | 74055042 | ID=MS.gene06871.t1.exon1;Parent=MS.gene06871.t1 |
| chr3.1 | CDS | 74055042 | 74055120 | 74055042 | ID=cds.MS.gene06871.t1;Parent=MS.gene06871.t1 |
| chr3.1 | exon | 74056148 | 74056307 | 74056148 | ID=MS.gene06871.t1.exon2;Parent=MS.gene06871.t1 |
| chr3.1 | CDS | 74056148 | 74056307 | 74056148 | ID=cds.MS.gene06871.t1;Parent=MS.gene06871.t1 |
| chr3.1 | exon | 74056739 | 74056988 | 74056739 | ID=MS.gene06871.t1.exon3;Parent=MS.gene06871.t1 |
| chr3.1 | CDS | 74056739 | 74056988 | 74056739 | ID=cds.MS.gene06871.t1;Parent=MS.gene06871.t1 |
| Gene Sequence |
| Protein sequence |
