Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070033.t1 | RHN42191.1 | 68.1 | 207 | 66 | 0 | 17 | 223 | 70 | 276 | 5.00E-73 | 284.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070033.t1 | A0A072TUI3 | 68.1 | 207 | 66 | 0 | 17 | 223 | 349 | 555 | 3.6e-73 | 284.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049877 | MS.gene070033 | 0.812327 | 4.48E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene070033 | 0.800403 | 1.47E-48 | -1.69E-46 |
| MS.gene052584 | MS.gene070033 | 0.80718 | 5.73E-50 | -1.69E-46 |
| MS.gene053396 | MS.gene070033 | 0.81188 | 5.61E-51 | -1.69E-46 |
| MS.gene053883 | MS.gene070033 | 0.828637 | 8.05E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene070033 | 0.835456 | 1.66E-56 | -1.69E-46 |
| MS.gene05423 | MS.gene070033 | 0.819748 | 9.87E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene070033 | 0.807486 | 4.94E-50 | -1.69E-46 |
| MS.gene056498 | MS.gene070033 | 0.807278 | 5.47E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene070033 | 0.816948 | 4.25E-52 | -1.69E-46 |
| MS.gene057414 | MS.gene070033 | 0.826239 | 3.02E-54 | -1.69E-46 |
| MS.gene059460 | MS.gene070033 | 0.802095 | 6.60E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene070033 | 0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene070033 | 0.804182 | 2.44E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene070033 | 0.803978 | 2.69E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene070033 | 0.816107 | 6.56E-52 | -1.69E-46 |
| MS.gene060634 | MS.gene070033 | -0.8306 | 2.68E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070033.t1 | MTR_2g008650 | 68.116 | 207 | 66 | 0 | 17 | 223 | 349 | 555 | 3.90e-96 | 291 |
| MS.gene070033.t1 | MTR_8g479260 | 68.116 | 207 | 66 | 0 | 17 | 223 | 349 | 555 | 3.90e-96 | 291 |
| MS.gene070033.t1 | MTR_4g036240 | 44.240 | 217 | 108 | 3 | 17 | 222 | 202 | 416 | 9.32e-47 | 159 |
| MS.gene070033.t1 | MTR_4g036240 | 44.240 | 217 | 108 | 3 | 17 | 222 | 265 | 479 | 3.80e-46 | 159 |
| MS.gene070033.t1 | MTR_4g036240 | 44.240 | 217 | 108 | 3 | 17 | 222 | 345 | 559 | 1.35e-45 | 159 |
| MS.gene070033.t1 | MTR_3g056270 | 43.779 | 217 | 109 | 3 | 17 | 222 | 504 | 718 | 2.01e-44 | 157 |
| MS.gene070033.t1 | MTR_2g048560 | 28.837 | 215 | 133 | 8 | 17 | 223 | 347 | 549 | 1.87e-18 | 83.6 |
| MS.gene070033.t1 | MTR_6g445520 | 46.429 | 84 | 44 | 1 | 17 | 100 | 212 | 294 | 2.37e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTCTGCAACTACAAATTT+TGG | 0.195858 | 4.3:-76065416 | None:intergenic |
| CCTGAGATTGCTGGACTTAT+TGG | 0.206628 | 4.3:+76064769 | MS.gene070033:CDS |
| CCCACTTTGTCATAAACTTC+TGG | 0.233927 | 4.3:-76064891 | None:intergenic |
| GATCCATACCATTGTTTGTT+TGG | 0.327116 | 4.3:-76064935 | None:intergenic |
| TGTGAAAATTGAAAAGCCTT+AGG | 0.358702 | 4.3:+76065553 | MS.gene070033:CDS |
| TGGGATATCTCCACTACATT+AGG | 0.359021 | 4.3:-76065443 | None:intergenic |
| TGGTCAAACTTTCCTCTTCA+AGG | 0.365164 | 4.3:+76064789 | MS.gene070033:CDS |
| CAGTTTCCACCTGAGATTGC+TGG | 0.367324 | 4.3:+76064760 | MS.gene070033:CDS |
| CTTCTTCATCATATAGTAAG+AGG | 0.389420 | 4.3:+76065471 | MS.gene070033:CDS |
| ATAAACTTCTGGATAATATC+AGG | 0.406812 | 4.3:-76064880 | None:intergenic |
| CGGAATGACTGCTTAAATCT+TGG | 0.414453 | 4.3:-76064835 | None:intergenic |
| GGGAAAACTCCTTTGATTGT+TGG | 0.417060 | 4.3:+76065096 | MS.gene070033:CDS |
| GTATTATACCATTCCATGTT+CGG | 0.418045 | 4.3:-76064640 | None:intergenic |
| TCTTGGAGAAAAGTCAGCTT+TGG | 0.420263 | 4.3:-76064818 | None:intergenic |
| GTGAAAATTGAAAAGCCTTA+GGG | 0.447822 | 4.3:+76065554 | MS.gene070033:CDS |
| TTTGCCTTGAACTTATCAAC+AGG | 0.449015 | 4.3:-76065265 | None:intergenic |
| TGAACCTGTTGATAAGTTCA+AGG | 0.467888 | 4.3:+76065261 | MS.gene070033:CDS |
| CCAATAAGTCCAGCAATCTC+AGG | 0.487592 | 4.3:-76064769 | None:intergenic |
| GTTCACGTCATCTTCATCCT+TGG | 0.493076 | 4.3:-76065504 | None:intergenic |
| TATATGATGAAGAAGATTTG+TGG | 0.500780 | 4.3:-76065463 | None:intergenic |
| AAGTTCAAGGCAAAGATTGT+GGG | 0.505271 | 4.3:+76065274 | MS.gene070033:CDS |
| TTATGTTTAGGCTATAGCTG+AGG | 0.513874 | 4.3:+76064735 | MS.gene070033:intron |
| TTAGAAATCCTATTAACTAA+AGG | 0.520550 | 4.3:-76064522 | None:intergenic |
| ATATGATGAAGAAGATTTGT+GGG | 0.520793 | 4.3:-76065462 | None:intergenic |
| TTTGAAAAGCCAACAATCAA+AGG | 0.531081 | 4.3:-76065105 | None:intergenic |
| TAAGAGGGAATCTCCTACCA+AGG | 0.543687 | 4.3:+76065487 | MS.gene070033:CDS |
| TATGTTTAGGCTATAGCTGA+GGG | 0.547043 | 4.3:+76064736 | MS.gene070033:intron |
| CATGCTCGCCGAACATGGAA+TGG | 0.550360 | 4.3:+76064632 | MS.gene070033:CDS |
| TCACACTAATTATGCTACAT+TGG | 0.553106 | 4.3:+76064560 | MS.gene070033:CDS |
| TCCAGAAGTTTATGACAAAG+TGG | 0.555990 | 4.3:+76064890 | MS.gene070033:CDS |
| CTTTGGTCTCCACCTTGAAG+AGG | 0.557017 | 4.3:-76064801 | None:intergenic |
| TAAGTTCAAGGCAAAGATTG+TGG | 0.573651 | 4.3:+76065273 | MS.gene070033:CDS |
| GTGCTCTGGTGGTGTCACAC+AGG | 0.577812 | 4.3:+76065300 | MS.gene070033:CDS |
| TTCATTCTCCAAACAAACAA+TGG | 0.582668 | 4.3:+76064927 | MS.gene070033:CDS |
| ACGTCATCTTCATCCTTGGT+AGG | 0.583600 | 4.3:-76065500 | None:intergenic |
| CCAGAAGTTTATGACAAAGT+GGG | 0.585100 | 4.3:+76064891 | MS.gene070033:CDS |
| GCTGACATGCTCGCCGAACA+TGG | 0.593916 | 4.3:+76064627 | MS.gene070033:CDS |
| TTAAGCAGTCATTCCGCGTG+AGG | 0.597360 | 4.3:+76064842 | MS.gene070033:CDS |
| TTCTTCATCATATAGTAAGA+GGG | 0.608686 | 4.3:+76065472 | MS.gene070033:CDS |
| TCAAACTTTCCTCTTCAAGG+TGG | 0.634274 | 4.3:+76064792 | MS.gene070033:CDS |
| AGTTCAAGGCAAAGATTGTG+GGG | 0.636594 | 4.3:+76065275 | MS.gene070033:CDS |
| GTCTTCTTGAGCATCTTGAG+TGG | 0.638886 | 4.3:-76065530 | None:intergenic |
| ATAAGTCCAGCAATCTCAGG+TGG | 0.645570 | 4.3:-76064766 | None:intergenic |
| CAACACAAATCTTCCTCACG+CGG | 0.659445 | 4.3:-76064855 | None:intergenic |
| AAGTTTATGACAAAGTGGGA+AGG | 0.660678 | 4.3:+76064895 | MS.gene070033:CDS |
| GTTCAAGGCAAAGATTGTGG+GGG | 0.703984 | 4.3:+76065276 | MS.gene070033:CDS |
| AGAATGTGAGCCTAATGTAG+TGG | 0.713457 | 4.3:+76065433 | MS.gene070033:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTTAAATCAAAAATCATT+AGG | + | chr4.3:76065185-76065204 | MS.gene070033:intron | 15.0% |
| !!! | ACAAAAAACTGTATTTTTTT+TGG | + | chr4.3:76064979-76064998 | MS.gene070033:intron | 15.0% |
| !!! | CAAAAAACTGTATTTTTTTT+GGG | + | chr4.3:76064980-76064999 | MS.gene070033:intron | 15.0% |
| !!! | GATAGATATATGTTATTTAA+GGG | - | chr4.3:76065213-76065232 | None:intergenic | 15.0% |
| !!! | GTATGTATTTTTTTATGTTT+AGG | + | chr4.3:76064723-76064742 | MS.gene070033:intron | 15.0% |
| !! | TTAGAAATCCTATTAACTAA+AGG | - | chr4.3:76064525-76064544 | None:intergenic | 20.0% |
| !!! | GGATAGATATATGTTATTTA+AGG | - | chr4.3:76065214-76065233 | None:intergenic | 20.0% |
| !!! | TTTGCATTGTTTTATGTTTT+AGG | + | chr4.3:76065041-76065060 | MS.gene070033:intron | 20.0% |
| ! | ATAAACTTCTGGATAATATC+AGG | - | chr4.3:76064883-76064902 | None:intergenic | 25.0% |
| ! | ATATGATGAAGAAGATTTGT+GGG | - | chr4.3:76065465-76065484 | None:intergenic | 25.0% |
| ! | CAGTTCAAATTTCTGTTTAA+AGG | + | chr4.3:76064673-76064692 | MS.gene070033:intron | 25.0% |
| ! | TATATGATGAAGAAGATTTG+TGG | - | chr4.3:76065466-76065485 | None:intergenic | 25.0% |
| ! | TTCTTCATCATATAGTAAGA+GGG | + | chr4.3:76065472-76065491 | MS.gene070033:CDS | 25.0% |
| ! | TTTGTATGCTTTATAACATG+AGG | + | chr4.3:76065161-76065180 | MS.gene070033:intron | 25.0% |
| !! | AGGTTTTACCTTTAGTTAAT+AGG | + | chr4.3:76064514-76064533 | MS.gene070033:CDS | 25.0% |
| !! | ATTTTCTCTAAGTGTTTTGT+TGG | + | chr4.3:76065068-76065087 | MS.gene070033:CDS | 25.0% |
| !!! | TACATTGGTAATTTTTGACA+AGG | + | chr4.3:76064575-76064594 | MS.gene070033:CDS | 25.0% |
| ATATATCTATCCCATTGTGT+AGG | + | chr4.3:76065222-76065241 | MS.gene070033:intron | 30.0% | |
| CTTCTTCATCATATAGTAAG+AGG | + | chr4.3:76065471-76065490 | MS.gene070033:CDS | 30.0% | |
| GTATTATACCATTCCATGTT+CGG | - | chr4.3:76064643-76064662 | None:intergenic | 30.0% | |
| TCACACTAATTATGCTACAT+TGG | + | chr4.3:76064560-76064579 | MS.gene070033:CDS | 30.0% | |
| TTCATTCTCCAAACAAACAA+TGG | + | chr4.3:76064927-76064946 | MS.gene070033:CDS | 30.0% | |
| TTTGAAAAGCCAACAATCAA+AGG | - | chr4.3:76065108-76065127 | None:intergenic | 30.0% | |
| ! | CATTCTGCAACTACAAATTT+TGG | - | chr4.3:76065419-76065438 | None:intergenic | 30.0% |
| !! | ATTGGTAATTTTTGACAAGG+AGG | + | chr4.3:76064578-76064597 | MS.gene070033:CDS | 30.0% |
| AAGTTCAAGGCAAAGATTGT+GGG | + | chr4.3:76065274-76065293 | MS.gene070033:CDS | 35.0% | |
| AAGTTTATGACAAAGTGGGA+AGG | + | chr4.3:76064895-76064914 | MS.gene070033:CDS | 35.0% | |
| CCAGAAGTTTATGACAAAGT+GGG | + | chr4.3:76064891-76064910 | MS.gene070033:CDS | 35.0% | |
| GATATGTCATCCTACACAAT+GGG | - | chr4.3:76065235-76065254 | None:intergenic | 35.0% | |
| GATCCATACCATTGTTTGTT+TGG | - | chr4.3:76064938-76064957 | None:intergenic | 35.0% | |
| TAAGTTCAAGGCAAAGATTG+TGG | + | chr4.3:76065273-76065292 | MS.gene070033:CDS | 35.0% | |
| TATGTTTAGGCTATAGCTGA+GGG | + | chr4.3:76064736-76064755 | MS.gene070033:intron | 35.0% | |
| TCCAGAAGTTTATGACAAAG+TGG | + | chr4.3:76064890-76064909 | MS.gene070033:CDS | 35.0% | |
| TCTCCAAACAAACAATGGTA+TGG | + | chr4.3:76064932-76064951 | MS.gene070033:intron | 35.0% | |
| TGATATGTCATCCTACACAA+TGG | - | chr4.3:76065236-76065255 | None:intergenic | 35.0% | |
| TTATGTTTAGGCTATAGCTG+AGG | + | chr4.3:76064735-76064754 | MS.gene070033:intron | 35.0% | |
| TTTGCCTTGAACTTATCAAC+AGG | - | chr4.3:76065268-76065287 | None:intergenic | 35.0% | |
| ! | TGAACCTGTTGATAAGTTCA+AGG | + | chr4.3:76065261-76065280 | MS.gene070033:CDS | 35.0% |
| !! | CTAAGTGTTTTGTTGGATGA+GGG | + | chr4.3:76065075-76065094 | MS.gene070033:CDS | 35.0% |
| !! | GACTTAGTTGACTTGATTTC+AGG | + | chr4.3:76065385-76065404 | MS.gene070033:intron | 35.0% |
| !! | TAAGTGTTTTGTTGGATGAG+GGG | + | chr4.3:76065076-76065095 | MS.gene070033:CDS | 35.0% |
| !! | TCTAAGTGTTTTGTTGGATG+AGG | + | chr4.3:76065074-76065093 | MS.gene070033:CDS | 35.0% |
| AGAATGTGAGCCTAATGTAG+TGG | + | chr4.3:76065433-76065452 | MS.gene070033:CDS | 40.0% | |
| AGTTCAAGGCAAAGATTGTG+GGG | + | chr4.3:76065275-76065294 | MS.gene070033:CDS | 40.0% | |
| CCCACTTTGTCATAAACTTC+TGG | - | chr4.3:76064894-76064913 | None:intergenic | 40.0% | |
| CGGAATGACTGCTTAAATCT+TGG | - | chr4.3:76064838-76064857 | None:intergenic | 40.0% | |
| GCTTGATACACAATGAGATC+TGG | + | chr4.3:76065002-76065021 | MS.gene070033:intron | 40.0% | |
| GGGAAAACTCCTTTGATTGT+TGG | + | chr4.3:76065096-76065115 | MS.gene070033:CDS | 40.0% | |
| TCAAACTTTCCTCTTCAAGG+TGG | + | chr4.3:76064792-76064811 | MS.gene070033:CDS | 40.0% | |
| TCTTGGAGAAAAGTCAGCTT+TGG | - | chr4.3:76064821-76064840 | None:intergenic | 40.0% | |
| TGGGATATCTCCACTACATT+AGG | - | chr4.3:76065446-76065465 | None:intergenic | 40.0% | |
| TGGTCAAACTTTCCTCTTCA+AGG | + | chr4.3:76064789-76064808 | MS.gene070033:CDS | 40.0% | |
| ACGTCATCTTCATCCTTGGT+AGG | - | chr4.3:76065503-76065522 | None:intergenic | 45.0% | |
| ATAAGTCCAGCAATCTCAGG+TGG | - | chr4.3:76064769-76064788 | None:intergenic | 45.0% | |
| CAACACAAATCTTCCTCACG+CGG | - | chr4.3:76064858-76064877 | None:intergenic | 45.0% | |
| CCAATAAGTCCAGCAATCTC+AGG | - | chr4.3:76064772-76064791 | None:intergenic | 45.0% | |
| CCTGAGATTGCTGGACTTAT+TGG | + | chr4.3:76064769-76064788 | MS.gene070033:CDS | 45.0% | |
| GTCTTCTTGAGCATCTTGAG+TGG | - | chr4.3:76065533-76065552 | None:intergenic | 45.0% | |
| GTTCAAGGCAAAGATTGTGG+GGG | + | chr4.3:76065276-76065295 | MS.gene070033:CDS | 45.0% | |
| GTTCACGTCATCTTCATCCT+TGG | - | chr4.3:76065507-76065526 | None:intergenic | 45.0% | |
| TAAGAGGGAATCTCCTACCA+AGG | + | chr4.3:76065487-76065506 | MS.gene070033:CDS | 45.0% | |
| CAGTTTCCACCTGAGATTGC+TGG | + | chr4.3:76064760-76064779 | MS.gene070033:CDS | 50.0% | |
| CTTTGGTCTCCACCTTGAAG+AGG | - | chr4.3:76064804-76064823 | None:intergenic | 50.0% | |
| TTAAGCAGTCATTCCGCGTG+AGG | + | chr4.3:76064842-76064861 | MS.gene070033:CDS | 50.0% | |
| CATGCTCGCCGAACATGGAA+TGG | + | chr4.3:76064632-76064651 | MS.gene070033:CDS | 55.0% | |
| !! | AAGATTGTGGGGGAGTGCTC+TGG | + | chr4.3:76065286-76065305 | MS.gene070033:CDS | 55.0% |
| GCTGACATGCTCGCCGAACA+TGG | + | chr4.3:76064627-76064646 | MS.gene070033:CDS | 60.0% | |
| !! | ATTGTGGGGGAGTGCTCTGG+TGG | + | chr4.3:76065289-76065308 | MS.gene070033:CDS | 60.0% |
| !! | GTGCTCTGGTGGTGTCACAC+AGG | + | chr4.3:76065300-76065319 | MS.gene070033:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 76064510 | 76065574 | 76064510 | ID=MS.gene070033 |
| chr4.3 | mRNA | 76064510 | 76065574 | 76064510 | ID=MS.gene070033.t1;Parent=MS.gene070033 |
| chr4.3 | exon | 76064510 | 76064653 | 76064510 | ID=MS.gene070033.t1.exon1;Parent=MS.gene070033.t1 |
| chr4.3 | CDS | 76064510 | 76064653 | 76064510 | ID=cds.MS.gene070033.t1;Parent=MS.gene070033.t1 |
| chr4.3 | exon | 76064745 | 76064948 | 76064745 | ID=MS.gene070033.t1.exon2;Parent=MS.gene070033.t1 |
| chr4.3 | CDS | 76064745 | 76064948 | 76064745 | ID=cds.MS.gene070033.t1;Parent=MS.gene070033.t1 |
| chr4.3 | exon | 76065063 | 76065140 | 76065063 | ID=MS.gene070033.t1.exon3;Parent=MS.gene070033.t1 |
| chr4.3 | CDS | 76065063 | 76065140 | 76065063 | ID=cds.MS.gene070033.t1;Parent=MS.gene070033.t1 |
| chr4.3 | exon | 76065244 | 76065321 | 76065244 | ID=MS.gene070033.t1.exon4;Parent=MS.gene070033.t1 |
| chr4.3 | CDS | 76065244 | 76065321 | 76065244 | ID=cds.MS.gene070033.t1;Parent=MS.gene070033.t1 |
| chr4.3 | exon | 76065407 | 76065574 | 76065407 | ID=MS.gene070033.t1.exon5;Parent=MS.gene070033.t1 |
| chr4.3 | CDS | 76065407 | 76065574 | 76065407 | ID=cds.MS.gene070033.t1;Parent=MS.gene070033.t1 |
| Gene Sequence |
| Protein sequence |
