Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070257.t1 | XP_003610095.1 | 96.5 | 172 | 6 | 0 | 1 | 172 | 1 | 172 | 5.00E-97 | 363.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070257.t1 | O64416 | 64.0 | 136 | 48 | 1 | 34 | 168 | 44 | 179 | 5.0e-53 | 208.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070257.t1 | G7JVD3 | 96.5 | 172 | 6 | 0 | 1 | 172 | 1 | 172 | 3.6e-97 | 363.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050971 | MS.gene070257 | -0.815819 | 7.60E-52 | -1.69E-46 |
| MS.gene051158 | MS.gene070257 | 0.801144 | 1.03E-48 | -1.69E-46 |
| MS.gene052128 | MS.gene070257 | 0.812745 | 3.63E-51 | -1.69E-46 |
| MS.gene052223 | MS.gene070257 | 0.802027 | 6.82E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene070257 | -0.803794 | 2.94E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene070257 | 0.819887 | 9.17E-53 | -1.69E-46 |
| MS.gene055601 | MS.gene070257 | 0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene056330 | MS.gene070257 | 0.822422 | 2.39E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene070257 | 0.828215 | 1.02E-54 | -1.69E-46 |
| MS.gene058218 | MS.gene070257 | 0.830998 | 2.14E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene070257 | -0.812372 | 4.38E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene070257 | 0.831403 | 1.70E-55 | -1.69E-46 |
| MS.gene059294 | MS.gene070257 | -0.804952 | 1.69E-49 | -1.69E-46 |
| MS.gene059402 | MS.gene070257 | 0.821119 | 4.78E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070257.t1 | MTR_4g127870 | 96.512 | 172 | 6 | 0 | 1 | 172 | 1 | 172 | 6.57e-127 | 353 |
| MS.gene070257.t1 | MTR_6g018300 | 51.220 | 164 | 76 | 3 | 7 | 169 | 16 | 176 | 1.65e-61 | 188 |
| MS.gene070257.t1 | MTR_6g018310 | 50.000 | 168 | 80 | 3 | 3 | 169 | 15 | 179 | 1.73e-61 | 188 |
| MS.gene070257.t1 | MTR_7g007120 | 51.282 | 156 | 72 | 3 | 15 | 169 | 28 | 180 | 1.04e-58 | 181 |
| MS.gene070257.t1 | MTR_7g007120 | 51.948 | 154 | 70 | 3 | 15 | 167 | 28 | 178 | 1.39e-58 | 181 |
| MS.gene070257.t1 | MTR_7g007220 | 51.948 | 154 | 70 | 3 | 15 | 167 | 28 | 178 | 1.60e-58 | 181 |
| MS.gene070257.t1 | MTR_7g007120 | 51.948 | 154 | 70 | 3 | 15 | 167 | 28 | 178 | 1.74e-58 | 181 |
| MS.gene070257.t1 | MTR_7g007200 | 51.948 | 154 | 70 | 3 | 15 | 167 | 28 | 178 | 2.10e-58 | 181 |
| MS.gene070257.t1 | MTR_7g007230 | 51.299 | 154 | 71 | 3 | 15 | 167 | 28 | 178 | 2.24e-57 | 178 |
| MS.gene070257.t1 | MTR_7g007210 | 52.857 | 140 | 62 | 3 | 15 | 153 | 28 | 164 | 1.56e-52 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070257.t1 | AT1G67090 | 50.000 | 172 | 82 | 3 | 1 | 171 | 11 | 179 | 7.74e-62 | 189 |
| MS.gene070257.t1 | AT5G38410 | 52.121 | 165 | 75 | 3 | 8 | 171 | 18 | 179 | 8.63e-62 | 189 |
| MS.gene070257.t1 | AT5G38420 | 52.727 | 165 | 74 | 3 | 8 | 171 | 18 | 179 | 8.72e-62 | 189 |
| MS.gene070257.t1 | AT5G38430 | 52.121 | 165 | 75 | 3 | 8 | 171 | 18 | 179 | 2.23e-61 | 188 |
| MS.gene070257.t1 | AT5G38430 | 52.121 | 165 | 75 | 3 | 8 | 171 | 18 | 179 | 2.23e-61 | 188 |
| MS.gene070257.t1 | AT5G38410 | 50.000 | 170 | 76 | 3 | 8 | 171 | 18 | 184 | 2.71e-60 | 186 |
| MS.gene070257.t1 | AT5G38410 | 51.220 | 164 | 71 | 4 | 8 | 171 | 18 | 172 | 2.70e-58 | 180 |
| MS.gene070257.t1 | AT1G67090 | 39.640 | 111 | 64 | 2 | 1 | 111 | 11 | 118 | 3.50e-22 | 87.0 |
Find 45 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAATTTCCTATTATTAA+TGG | 0.204312 | 8.2:+5959887 | None:intergenic |
| ATCAGAAAGGGGTGGTAAAT+AGG | 0.291264 | 8.2:+5959857 | None:intergenic |
| ATGGGAGGTATTGGACATTA+TGG | 0.311655 | 8.2:-5959250 | MS.gene070257:CDS |
| TCAAATTTCCTATTATTAAT+GGG | 0.315443 | 8.2:+5959888 | None:intergenic |
| CATGGAATCCCATTAATAAT+AGG | 0.318513 | 8.2:-5959896 | MS.gene070257:CDS |
| GCAGCTTCAGTTGCTGGGCC+TGG | 0.319427 | 8.2:-5960169 | MS.gene070257:CDS |
| GTTGCTGGGCCTGGCTTTGT+GGG | 0.327668 | 8.2:-5960160 | MS.gene070257:CDS |
| AGTCCAACTCATCAAATCTT+TGG | 0.338452 | 8.2:-5960132 | MS.gene070257:CDS |
| ACATTTGCAGCTTCAGTTGC+TGG | 0.348801 | 8.2:-5960175 | MS.gene070257:CDS |
| AGTTGCTGGGCCTGGCTTTG+TGG | 0.368695 | 8.2:-5960161 | MS.gene070257:CDS |
| AAGCATCTTATATATGCATT+TGG | 0.378298 | 8.2:+5959155 | None:intergenic |
| TAAGTAGTTGTTGTGGTAGT+AGG | 0.382906 | 8.2:+5959080 | None:intergenic |
| TTCAATGGAATAAGTAGTAC+TGG | 0.385537 | 8.2:+5960108 | None:intergenic |
| CATTTGCAGCTTCAGTTGCT+GGG | 0.394890 | 8.2:-5960174 | MS.gene070257:CDS |
| GATACTATGATGGGAGGTAT+TGG | 0.398812 | 8.2:-5959259 | MS.gene070257:CDS |
| GTACTACTTATTCCATTGAA+TGG | 0.406489 | 8.2:-5960105 | MS.gene070257:CDS |
| TTATGGAAGCTACCAATGTT+TGG | 0.409185 | 8.2:-5959233 | MS.gene070257:CDS |
| GACTTCAAGCCCACAAAGCC+AGG | 0.438256 | 8.2:+5960151 | None:intergenic |
| TGCAATTGAGTCATCAGAAA+GGG | 0.441885 | 8.2:+5959845 | None:intergenic |
| GACTAACCTCATCAAATTCA+AGG | 0.447817 | 8.2:+5959781 | None:intergenic |
| CATCATAGTATCCTGGCATC+TGG | 0.482846 | 8.2:+5959270 | None:intergenic |
| GCAGTGAATCTTAGAACCAT+TGG | 0.489711 | 8.2:+5960066 | None:intergenic |
| CAGATGCCAGGATACTATGA+TGG | 0.514747 | 8.2:-5959269 | MS.gene070257:CDS |
| GGGCCAAAGATTTGATGAGT+TGG | 0.537281 | 8.2:+5960129 | None:intergenic |
| GATTGACTACATGCTCAAGA+AGG | 0.539499 | 8.2:-5959817 | MS.gene070257:CDS |
| TACCTCCCATCATAGTATCC+TGG | 0.541106 | 8.2:+5959263 | None:intergenic |
| TTCTGATGACTCAATTGCAA+AGG | 0.545642 | 8.2:-5959841 | MS.gene070257:CDS |
| CAAGCGCCACATGCAAGCCA+TGG | 0.545894 | 8.2:-5959120 | MS.gene070257:CDS |
| TTTGTGAATAATAAAAGCCA+TGG | 0.556871 | 8.2:+5959103 | None:intergenic |
| TTCTAAGATTCACTGCATGA+AGG | 0.557279 | 8.2:-5960059 | MS.gene070257:intron |
| TTGCAATTGAGTCATCAGAA+AGG | 0.572127 | 8.2:+5959844 | None:intergenic |
| AGAGCAAATAGCCAGATGCC+AGG | 0.574689 | 8.2:-5959281 | MS.gene070257:CDS |
| ACCTCATCAAATTCAAGGCA+AGG | 0.584505 | 8.2:+5959786 | None:intergenic |
| AGATGCCAGGATACTATGAT+GGG | 0.593649 | 8.2:-5959268 | MS.gene070257:CDS |
| ATTGACTACATGCTCAAGAA+GGG | 0.603348 | 8.2:-5959816 | MS.gene070257:CDS |
| ACCTTGCCTTGAATTTGATG+AGG | 0.604745 | 8.2:-5959787 | MS.gene070257:intron |
| ACTACATGCTCAAGAAGGGA+TGG | 0.608538 | 8.2:-5959812 | MS.gene070257:CDS |
| GATTAATTAAGTAGTTGTTG+TGG | 0.614403 | 8.2:+5959073 | None:intergenic |
| TAAAAGCCATGGCTTGCATG+TGG | 0.625954 | 8.2:+5959114 | None:intergenic |
| GCAATTGAGTCATCAGAAAG+GGG | 0.629169 | 8.2:+5959846 | None:intergenic |
| TCAATGGAATAAGTAGTACT+GGG | 0.635347 | 8.2:+5960109 | None:intergenic |
| AATCTTAGAACCATTGGAGA+CGG | 0.640552 | 8.2:+5960072 | None:intergenic |
| TGCCAGGATACTATGATGGG+AGG | 0.662604 | 8.2:-5959265 | MS.gene070257:CDS |
| GAGTCGTTACAACCAAACAT+TGG | 0.684048 | 8.2:+5959221 | None:intergenic |
| ATTGAGTCATCAGAAAGGGG+TGG | 0.697277 | 8.2:+5959849 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTATATCTTATTAATAATA+TGG | - | chr8.2:5959922-5959941 | MS.gene070257:intron | 10.0% |
| !! | TTATATTATAAACTAGATTT+AGG | + | chr8.2:5959514-5959533 | None:intergenic | 10.0% |
| !!! | GTAGTTTTTTTATTATTATA+AGG | - | chr8.2:5959535-5959554 | MS.gene070257:intron | 10.0% |
| !!! | TAAATCTAGTTTATAATATA+AGG | - | chr8.2:5959513-5959532 | MS.gene070257:intron | 10.0% |
| !! | ATTATAAGGAAAAAACAATT+AGG | - | chr8.2:5959549-5959568 | MS.gene070257:intron | 15.0% |
| !! | GATTCATATAATATAGATTA+AGG | - | chr8.2:5959602-5959621 | MS.gene070257:intron | 15.0% |
| !! | TCAAATTTCCTATTATTAAT+GGG | + | chr8.2:5959381-5959400 | None:intergenic | 15.0% |
| !! | TTCAAATTTCCTATTATTAA+TGG | + | chr8.2:5959382-5959401 | None:intergenic | 15.0% |
| !!! | AAGTTATACAAAAGTTTTAT+TGG | + | chr8.2:5959840-5959859 | None:intergenic | 15.0% |
| !! | ATTATAAACTAGATTTAGGT+TGG | + | chr8.2:5959510-5959529 | None:intergenic | 20.0% |
| !! | TAGAAAACAAAAACATCAAA+AGG | + | chr8.2:5959897-5959916 | None:intergenic | 20.0% |
| !!! | CTTTAATTTATTCTATGAAG+TGG | - | chr8.2:5959857-5959876 | MS.gene070257:CDS | 20.0% |
| ! | AACCATGCTATCATAATATA+TGG | + | chr8.2:5959254-5959273 | None:intergenic | 25.0% |
| ! | AAGCATCTTATATATGCATT+TGG | + | chr8.2:5960114-5960133 | None:intergenic | 25.0% |
| ! | AATATGGAACTTGTATTGTT+TGG | - | chr8.2:5959938-5959957 | MS.gene070257:intron | 25.0% |
| ! | ATATAGATTAAGGCAATGTT+TGG | - | chr8.2:5959612-5959631 | MS.gene070257:intron | 25.0% |
| ! | TTTGTAACTTAAAACAGACA+TGG | - | chr8.2:5959352-5959371 | MS.gene070257:intron | 25.0% |
| ! | TTTGTGAATAATAAAAGCCA+TGG | + | chr8.2:5960166-5960185 | None:intergenic | 25.0% |
| AGAAAAGAAAAAGACCAAGA+TGG | + | chr8.2:5959297-5959316 | None:intergenic | 30.0% | |
| AGCCATATATTATGATAGCA+TGG | - | chr8.2:5959249-5959268 | MS.gene070257:CDS | 30.0% | |
| ATTGTTTGGAAATGGAAGTT+TGG | - | chr8.2:5959952-5959971 | MS.gene070257:intron | 30.0% | |
| CATGGAATCCCATTAATAAT+AGG | - | chr8.2:5959370-5959389 | MS.gene070257:intron | 30.0% | |
| GAAAAGAAAAAGACCAAGAT+GGG | + | chr8.2:5959296-5959315 | None:intergenic | 30.0% | |
| GAACTTGTATTGTTTGGAAA+TGG | - | chr8.2:5959944-5959963 | MS.gene070257:intron | 30.0% | |
| GTACTACTTATTCCATTGAA+TGG | - | chr8.2:5959161-5959180 | MS.gene070257:CDS | 30.0% | |
| TCAATGGAATAAGTAGTACT+GGG | + | chr8.2:5959160-5959179 | None:intergenic | 30.0% | |
| TTCAATGGAATAAGTAGTAC+TGG | + | chr8.2:5959161-5959180 | None:intergenic | 30.0% | |
| AATCTTAGAACCATTGGAGA+CGG | + | chr8.2:5959197-5959216 | None:intergenic | 35.0% | |
| AGTCCAACTCATCAAATCTT+TGG | - | chr8.2:5959134-5959153 | MS.gene070257:CDS | 35.0% | |
| ATTGACTACATGCTCAAGAA+GGG | - | chr8.2:5959450-5959469 | MS.gene070257:intron | 35.0% | |
| GACTAACCTCATCAAATTCA+AGG | + | chr8.2:5959488-5959507 | None:intergenic | 35.0% | |
| TAAGTAGTTGTTGTGGTAGT+AGG | + | chr8.2:5960189-5960208 | None:intergenic | 35.0% | |
| TGCAATTGAGTCATCAGAAA+GGG | + | chr8.2:5959424-5959443 | None:intergenic | 35.0% | |
| TTATGGAAGCTACCAATGTT+TGG | - | chr8.2:5960033-5960052 | MS.gene070257:intron | 35.0% | |
| TTCTGATGACTCAATTGCAA+AGG | - | chr8.2:5959425-5959444 | MS.gene070257:intron | 35.0% | |
| TTGCAATTGAGTCATCAGAA+AGG | + | chr8.2:5959425-5959444 | None:intergenic | 35.0% | |
| ! | GGTCTTCTTTTTCCATTCAA+TGG | + | chr8.2:5959176-5959195 | None:intergenic | 35.0% |
| !! | TTCTAAGATTCACTGCATGA+AGG | - | chr8.2:5959207-5959226 | MS.gene070257:CDS | 35.0% |
| AAAAAGAAGACCGTCTCCAA+TGG | - | chr8.2:5959184-5959203 | MS.gene070257:CDS | 40.0% | |
| ACCTCATCAAATTCAAGGCA+AGG | + | chr8.2:5959483-5959502 | None:intergenic | 40.0% | |
| ACCTTGCCTTGAATTTGATG+AGG | - | chr8.2:5959479-5959498 | MS.gene070257:intron | 40.0% | |
| AGATGCCAGGATACTATGAT+GGG | - | chr8.2:5959998-5960017 | MS.gene070257:intron | 40.0% | |
| ATCAGAAAGGGGTGGTAAAT+AGG | + | chr8.2:5959412-5959431 | None:intergenic | 40.0% | |
| ATGTACTAGTACTCCCATCT+TGG | - | chr8.2:5959280-5959299 | MS.gene070257:CDS | 40.0% | |
| GAGTCGTTACAACCAAACAT+TGG | + | chr8.2:5960048-5960067 | None:intergenic | 40.0% | |
| GATTGACTACATGCTCAAGA+AGG | - | chr8.2:5959449-5959468 | MS.gene070257:intron | 40.0% | |
| GCAATTGAGTCATCAGAAAG+GGG | + | chr8.2:5959423-5959442 | None:intergenic | 40.0% | |
| GCAGTGAATCTTAGAACCAT+TGG | + | chr8.2:5959203-5959222 | None:intergenic | 40.0% | |
| ! | GATACTATGATGGGAGGTAT+TGG | - | chr8.2:5960007-5960026 | MS.gene070257:intron | 40.0% |
| !! | ATGGGAGGTATTGGACATTA+TGG | - | chr8.2:5960016-5960035 | MS.gene070257:intron | 40.0% |
| ACATTTGCAGCTTCAGTTGC+TGG | - | chr8.2:5959091-5959110 | MS.gene070257:CDS | 45.0% | |
| ACTACATGCTCAAGAAGGGA+TGG | - | chr8.2:5959454-5959473 | MS.gene070257:intron | 45.0% | |
| ATTGAGTCATCAGAAAGGGG+TGG | + | chr8.2:5959420-5959439 | None:intergenic | 45.0% | |
| CAGATGCCAGGATACTATGA+TGG | - | chr8.2:5959997-5960016 | MS.gene070257:intron | 45.0% | |
| CATCATAGTATCCTGGCATC+TGG | + | chr8.2:5959999-5960018 | None:intergenic | 45.0% | |
| CATTTGCAGCTTCAGTTGCT+GGG | - | chr8.2:5959092-5959111 | MS.gene070257:CDS | 45.0% | |
| GGGCCAAAGATTTGATGAGT+TGG | + | chr8.2:5959140-5959159 | None:intergenic | 45.0% | |
| TACCTCCCATCATAGTATCC+TGG | + | chr8.2:5960006-5960025 | None:intergenic | 45.0% | |
| ! | TAAAAGCCATGGCTTGCATG+TGG | + | chr8.2:5960155-5960174 | None:intergenic | 45.0% |
| AGAGCAAATAGCCAGATGCC+AGG | - | chr8.2:5959985-5960004 | MS.gene070257:intron | 50.0% | |
| ! | TGCCAGGATACTATGATGGG+AGG | - | chr8.2:5960001-5960020 | MS.gene070257:intron | 50.0% |
| GACTTCAAGCCCACAAAGCC+AGG | + | chr8.2:5959118-5959137 | None:intergenic | 55.0% | |
| CAAGCGCCACATGCAAGCCA+TGG | - | chr8.2:5960146-5960165 | MS.gene070257:CDS | 60.0% | |
| !! | AGTTGCTGGGCCTGGCTTTG+TGG | - | chr8.2:5959105-5959124 | MS.gene070257:CDS | 60.0% |
| !! | GTTGCTGGGCCTGGCTTTGT+GGG | - | chr8.2:5959106-5959125 | MS.gene070257:CDS | 60.0% |
| GCAGCTTCAGTTGCTGGGCC+TGG | - | chr8.2:5959097-5959116 | MS.gene070257:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 5959079 | 5960209 | 5959079 | ID=MS.gene070257 |
| chr8.2 | mRNA | 5959079 | 5960209 | 5959079 | ID=MS.gene070257.t1;Parent=MS.gene070257 |
| chr8.2 | exon | 5960060 | 5960209 | 5960060 | ID=MS.gene070257.t1.exon1;Parent=MS.gene070257.t1 |
| chr8.2 | CDS | 5960060 | 5960209 | 5960060 | ID=cds.MS.gene070257.t1;Parent=MS.gene070257.t1 |
| chr8.2 | exon | 5959788 | 5959919 | 5959788 | ID=MS.gene070257.t1.exon2;Parent=MS.gene070257.t1 |
| chr8.2 | CDS | 5959788 | 5959919 | 5959788 | ID=cds.MS.gene070257.t1;Parent=MS.gene070257.t1 |
| chr8.2 | exon | 5959079 | 5959318 | 5959079 | ID=MS.gene070257.t1.exon3;Parent=MS.gene070257.t1 |
| chr8.2 | CDS | 5959079 | 5959318 | 5959079 | ID=cds.MS.gene070257.t1;Parent=MS.gene070257.t1 |
| Gene Sequence |
| Protein sequence |
