Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070467.t1 | XP_025684871.1 | 59.6 | 327 | 127 | 2 | 1 | 322 | 473 | 799 | 1.30E-103 | 386.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070467.t1 | P08770 | 35.3 | 295 | 178 | 5 | 1 | 292 | 470 | 754 | 6.9e-40 | 166.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070467.t1 | A0A444X1G4 | 59.9 | 312 | 122 | 1 | 1 | 309 | 1 | 312 | 3.5e-101 | 377.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene070467 | 0.827733 | 1.33E-54 | -1.69E-46 |
| MS.gene051575 | MS.gene070467 | 0.803513 | 3.36E-49 | -1.69E-46 |
| MS.gene05329 | MS.gene070467 | 0.802678 | 5.01E-49 | -1.69E-46 |
| MS.gene054484 | MS.gene070467 | 0.80284 | 4.63E-49 | -1.69E-46 |
| MS.gene055056 | MS.gene070467 | 0.818715 | 1.70E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene070467 | 0.802212 | 6.24E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene070467 | 0.853204 | 2.79E-61 | -1.69E-46 |
| MS.gene059584 | MS.gene070467 | 0.808632 | 2.82E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070467.t1 | AT3G42170 | 28.028 | 289 | 187 | 6 | 1 | 288 | 383 | 651 | 1.10e-33 | 131 |
| MS.gene070467.t1 | AT3G42170 | 28.028 | 289 | 187 | 6 | 1 | 288 | 405 | 673 | 1.19e-33 | 131 |
| MS.gene070467.t1 | AT1G18560 | 24.775 | 222 | 139 | 6 | 73 | 288 | 449 | 648 | 2.11e-12 | 68.2 |
Find 50 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGGATCGACACCAATTT+TGG | 0.158761 | 3.3:+24831367 | None:intergenic |
| GATATTACTAACAAGGTTTC+TGG | 0.158954 | 3.3:-24832074 | MS.gene070467:CDS |
| ACAAATTGCATCTTGTTTCT+AGG | 0.170369 | 3.3:+24831861 | None:intergenic |
| TTGGGCAAGAATTGGATATT+TGG | 0.245664 | 3.3:+24831521 | None:intergenic |
| CACTAGTTTGTGCCCAAAAT+TGG | 0.262710 | 3.3:-24831380 | MS.gene070467:CDS |
| AACTCAATCTTCTAATGATA+TGG | 0.269191 | 3.3:-24831712 | MS.gene070467:CDS |
| ATCAGATGTTGAAGATCTTT+TGG | 0.279008 | 3.3:-24831337 | MS.gene070467:CDS |
| CAATTCAATATGTCAAAATT+AGG | 0.283111 | 3.3:+24831561 | None:intergenic |
| TTTGGAAAATGCATTGGATT+TGG | 0.310667 | 3.3:-24831670 | MS.gene070467:CDS |
| AGGTAAGTATGATAAATATT+GGG | 0.337982 | 3.3:-24831934 | MS.gene070467:CDS |
| AGTGGAAGAACTTGATGTTT+TGG | 0.338776 | 3.3:-24831688 | MS.gene070467:CDS |
| GCCTGTTTCTACTGTTGCTT+CGG | 0.344922 | 3.3:-24831475 | MS.gene070467:CDS |
| ACTAGTGCTTCAACTGTCTT+TGG | 0.347826 | 3.3:+24831396 | None:intergenic |
| AAGGTAAGTATGATAAATAT+TGG | 0.362831 | 3.3:-24831935 | MS.gene070467:CDS |
| CTAGCAACTTGGGCAAGAAT+TGG | 0.369016 | 3.3:+24831513 | None:intergenic |
| GTAGAGTGCTAAATTGTTAC+AGG | 0.377011 | 3.3:-24831431 | MS.gene070467:CDS |
| TCTTCAGTCAGCTGTGGTAT+TGG | 0.398254 | 3.3:+24831181 | None:intergenic |
| AGATATAAGCTTTGTATTCA+AGG | 0.410404 | 3.3:-24831750 | MS.gene070467:CDS |
| CCAACACATCTCTAGCAACT+TGG | 0.420043 | 3.3:+24831502 | None:intergenic |
| TCTTCAACATCTGATGCAAT+TGG | 0.422249 | 3.3:+24831345 | None:intergenic |
| ACTCCCTATCCATTTCTTGA+AGG | 0.438601 | 3.3:+24831995 | None:intergenic |
| TATTGACTACCTTCAAGAAA+TGG | 0.439729 | 3.3:-24832004 | MS.gene070467:CDS |
| ACAAGATGCAATTTGTTAAG+TGG | 0.463036 | 3.3:-24831854 | MS.gene070467:CDS |
| TCCGAAGCAACAGTAGAAAC+AGG | 0.468162 | 3.3:+24831474 | None:intergenic |
| GGCAAGAATTGGATATTTGG+TGG | 0.470268 | 3.3:+24831524 | None:intergenic |
| CAAGATGCAATTTGTTAAGT+GGG | 0.478466 | 3.3:-24831853 | MS.gene070467:CDS |
| GGGAGTTCTGACAAACATCT+TGG | 0.487268 | 3.3:-24831978 | MS.gene070467:CDS |
| CATGAATAAATGTTATGAAA+AGG | 0.491303 | 3.3:-24831829 | MS.gene070467:CDS |
| GGTAAGTATGATAAATATTG+GGG | 0.512358 | 3.3:-24831933 | MS.gene070467:CDS |
| ACTACCTTCAAGAAATGGAT+AGG | 0.517007 | 3.3:-24831999 | MS.gene070467:CDS |
| TGAAGTGGATTTGTATCTCA+TGG | 0.531319 | 3.3:-24831604 | MS.gene070467:CDS |
| AAACATCTTGGAGACATGGC+TGG | 0.534940 | 3.3:-24831966 | MS.gene070467:CDS |
| ATCTTCTAATGATATGGAAG+TGG | 0.541150 | 3.3:-24831706 | MS.gene070467:CDS |
| CAACACATCTCTAGCAACTT+GGG | 0.551817 | 3.3:+24831503 | None:intergenic |
| ACAATTTAGCACTCTACCTC+CGG | 0.555600 | 3.3:+24831437 | None:intergenic |
| CAGTGCAATTAAGTTTCAGA+AGG | 0.555821 | 3.3:-24832213 | MS.gene070467:CDS |
| GTGGATTTGTATCTCATGGA+GGG | 0.558666 | 3.3:-24831600 | MS.gene070467:CDS |
| TAATATGAATGTAAATGAAG+TGG | 0.560827 | 3.3:-24831619 | MS.gene070467:CDS |
| CCAAGTTGCTAGAGATGTGT+TGG | 0.581621 | 3.3:-24831502 | MS.gene070467:CDS |
| CTACCTTCAAGAAATGGATA+GGG | 0.591958 | 3.3:-24831998 | MS.gene070467:CDS |
| TGACATATTGAATTGGTGGA+AGG | 0.597517 | 3.3:-24831553 | MS.gene070467:CDS |
| TGACAAACATCTTGGAGACA+TGG | 0.601396 | 3.3:-24831970 | MS.gene070467:CDS |
| CTTGTTGATATATGTTGCAG+TGG | 0.608677 | 3.3:-24831892 | MS.gene070467:CDS |
| AACATCTGATGCAATTGGAG+AGG | 0.621951 | 3.3:+24831350 | None:intergenic |
| AAGATGCAATTTGTTAAGTG+GGG | 0.634337 | 3.3:-24831852 | MS.gene070467:CDS |
| GACATCTCTTCAGTCAGCTG+TGG | 0.639310 | 3.3:+24831175 | None:intergenic |
| GACATGGCTGGAATGATGAA+AGG | 0.644026 | 3.3:-24831954 | MS.gene070467:CDS |
| AGCAACAGTAGAAACAGGCA+TGG | 0.654712 | 3.3:+24831479 | None:intergenic |
| AGTGGATTTGTATCTCATGG+AGG | 0.666727 | 3.3:-24831601 | MS.gene070467:CDS |
| GTAAGTATGATAAATATTGG+GGG | 0.704348 | 3.3:-24831932 | MS.gene070467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATCATAAAATATTTTC+AGG | + | chr3.3:24831290-24831309 | None:intergenic | 10.0% |
| !!! | ATTTTATGATATTACTAACA+AGG | - | chr3.3:24831296-24831315 | MS.gene070467:intron | 15.0% |
| !! | AAGGTAAGTATGATAAATAT+TGG | - | chr3.3:24831442-24831461 | MS.gene070467:CDS | 20.0% |
| !! | AGGTAAGTATGATAAATATT+GGG | - | chr3.3:24831443-24831462 | MS.gene070467:CDS | 20.0% |
| !! | CAATTCAATATGTCAAAATT+AGG | + | chr3.3:24831819-24831838 | None:intergenic | 20.0% |
| !! | CATGAATAAATGTTATGAAA+AGG | - | chr3.3:24831548-24831567 | MS.gene070467:CDS | 20.0% |
| !! | TAATATGAATGTAAATGAAG+TGG | - | chr3.3:24831758-24831777 | MS.gene070467:CDS | 20.0% |
| !! | TCTTATTGTTGAATTTCTTA+TGG | - | chr3.3:24832082-24832101 | MS.gene070467:CDS | 20.0% |
| !!! | CTAATTTTGACATATTGAAT+TGG | - | chr3.3:24831817-24831836 | MS.gene070467:CDS | 20.0% |
| !!! | TATTTTCAGGAATTTAACAA+AGG | + | chr3.3:24831277-24831296 | None:intergenic | 20.0% |
| ! | AACTCAATCTTCTAATGATA+TGG | - | chr3.3:24831665-24831684 | MS.gene070467:CDS | 25.0% |
| ! | GGTAAGTATGATAAATATTG+GGG | - | chr3.3:24831444-24831463 | MS.gene070467:CDS | 25.0% |
| ! | GTAAGTATGATAAATATTGG+GGG | - | chr3.3:24831445-24831464 | MS.gene070467:CDS | 25.0% |
| !! | AGATATAAGCTTTGTATTCA+AGG | - | chr3.3:24831627-24831646 | MS.gene070467:CDS | 25.0% |
| !!! | ATTTTGACATATTGAATTGG+TGG | - | chr3.3:24831820-24831839 | MS.gene070467:CDS | 25.0% |
| !!! | TGTTGATTTTTTGTCTGATA+AGG | - | chr3.3:24831575-24831594 | MS.gene070467:CDS | 25.0% |
| !!! | TTTTTTGTCTGATAAGGTAA+AGG | - | chr3.3:24831581-24831600 | MS.gene070467:CDS | 25.0% |
| ATCTTCTAATGATATGGAAG+TGG | - | chr3.3:24831671-24831690 | MS.gene070467:CDS | 30.0% | |
| GATATTACTAACAAGGTTTC+TGG | - | chr3.3:24831303-24831322 | MS.gene070467:intron | 30.0% | |
| TTTGGAAAATGCATTGGATT+TGG | - | chr3.3:24831707-24831726 | MS.gene070467:CDS | 30.0% | |
| ! | ACAAATTGCATCTTGTTTCT+AGG | + | chr3.3:24831519-24831538 | None:intergenic | 30.0% |
| ! | ATCAGATGTTGAAGATCTTT+TGG | - | chr3.3:24832040-24832059 | MS.gene070467:CDS | 30.0% |
| ! | TATTGACTACCTTCAAGAAA+TGG | - | chr3.3:24831373-24831392 | MS.gene070467:CDS | 30.0% |
| !! | AAGATGCAATTTGTTAAGTG+GGG | - | chr3.3:24831525-24831544 | MS.gene070467:CDS | 30.0% |
| !! | ACAAGATGCAATTTGTTAAG+TGG | - | chr3.3:24831523-24831542 | MS.gene070467:CDS | 30.0% |
| !! | CAAGATGCAATTTGTTAAGT+GGG | - | chr3.3:24831524-24831543 | MS.gene070467:CDS | 30.0% |
| !!! | TGATGTTTTGGAAAATGCAT+TGG | - | chr3.3:24831701-24831720 | MS.gene070467:CDS | 30.0% |
| ACTACCTTCAAGAAATGGAT+AGG | - | chr3.3:24831378-24831397 | MS.gene070467:CDS | 35.0% | |
| CAGTGCAATTAAGTTTCAGA+AGG | - | chr3.3:24831164-24831183 | MS.gene070467:CDS | 35.0% | |
| CTACCTTCAAGAAATGGATA+GGG | - | chr3.3:24831379-24831398 | MS.gene070467:CDS | 35.0% | |
| CTTGTTGATATATGTTGCAG+TGG | - | chr3.3:24831485-24831504 | MS.gene070467:CDS | 35.0% | |
| GGTTCCAAAAAATGAGGATT+GGG | - | chr3.3:24831239-24831258 | MS.gene070467:intron | 35.0% | |
| TCTTCAACATCTGATGCAAT+TGG | + | chr3.3:24832035-24832054 | None:intergenic | 35.0% | |
| TGACATATTGAATTGGTGGA+AGG | - | chr3.3:24831824-24831843 | MS.gene070467:CDS | 35.0% | |
| TTGGGCAAGAATTGGATATT+TGG | + | chr3.3:24831859-24831878 | None:intergenic | 35.0% | |
| ! | AGTGGAAGAACTTGATGTTT+TGG | - | chr3.3:24831689-24831708 | MS.gene070467:CDS | 35.0% |
| ! | GTAGAGTGCTAAATTGTTAC+AGG | - | chr3.3:24831946-24831965 | MS.gene070467:CDS | 35.0% |
| ! | TGAAGTGGATTTGTATCTCA+TGG | - | chr3.3:24831773-24831792 | MS.gene070467:CDS | 35.0% |
| !!! | GTTGAAGATCTTTTGGAACT+AGG | - | chr3.3:24832047-24832066 | MS.gene070467:CDS | 35.0% |
| !!! | TTCTCCCAATCCTCATTTTT+TGG | + | chr3.3:24831246-24831265 | None:intergenic | 35.0% |
| AACATCTGATGCAATTGGAG+AGG | + | chr3.3:24832030-24832049 | None:intergenic | 40.0% | |
| ACAATTTAGCACTCTACCTC+CGG | + | chr3.3:24831943-24831962 | None:intergenic | 40.0% | |
| ACTAGTGCTTCAACTGTCTT+TGG | + | chr3.3:24831984-24832003 | None:intergenic | 40.0% | |
| ACTCCCTATCCATTTCTTGA+AGG | + | chr3.3:24831385-24831404 | None:intergenic | 40.0% | |
| CAACACATCTCTAGCAACTT+GGG | + | chr3.3:24831877-24831896 | None:intergenic | 40.0% | |
| GGCAAGAATTGGATATTTGG+TGG | + | chr3.3:24831856-24831875 | None:intergenic | 40.0% | |
| GGGTTCCAAAAAATGAGGAT+TGG | - | chr3.3:24831238-24831257 | MS.gene070467:intron | 40.0% | |
| GTGGATTTGTATCTCATGGA+GGG | - | chr3.3:24831777-24831796 | MS.gene070467:CDS | 40.0% | |
| TGACAAACATCTTGGAGACA+TGG | - | chr3.3:24831407-24831426 | MS.gene070467:CDS | 40.0% | |
| ! | AATTTTCGCGTATCGAAGGT+GGG | - | chr3.3:24831217-24831236 | MS.gene070467:intron | 40.0% |
| ! | AGAGGATCGACACCAATTTT+GGG | + | chr3.3:24832012-24832031 | None:intergenic | 40.0% |
| ! | AGTGGATTTGTATCTCATGG+AGG | - | chr3.3:24831776-24831795 | MS.gene070467:CDS | 40.0% |
| ! | CACTAGTTTGTGCCCAAAAT+TGG | - | chr3.3:24831997-24832016 | MS.gene070467:CDS | 40.0% |
| AAACATCTTGGAGACATGGC+TGG | - | chr3.3:24831411-24831430 | MS.gene070467:CDS | 45.0% | |
| AGCAACAGTAGAAACAGGCA+TGG | + | chr3.3:24831901-24831920 | None:intergenic | 45.0% | |
| AGGTGGGGTTCCAAAAAATG+AGG | - | chr3.3:24831233-24831252 | MS.gene070467:intron | 45.0% | |
| CCAACACATCTCTAGCAACT+TGG | + | chr3.3:24831878-24831897 | None:intergenic | 45.0% | |
| CCAAGTTGCTAGAGATGTGT+TGG | - | chr3.3:24831875-24831894 | MS.gene070467:CDS | 45.0% | |
| CTAGCAACTTGGGCAAGAAT+TGG | + | chr3.3:24831867-24831886 | None:intergenic | 45.0% | |
| GACATGGCTGGAATGATGAA+AGG | - | chr3.3:24831423-24831442 | MS.gene070467:CDS | 45.0% | |
| TCCGAAGCAACAGTAGAAAC+AGG | + | chr3.3:24831906-24831925 | None:intergenic | 45.0% | |
| TCTTCAGTCAGCTGTGGTAT+TGG | + | chr3.3:24832199-24832218 | None:intergenic | 45.0% | |
| ! | ATTTTCGCGTATCGAAGGTG+GGG | - | chr3.3:24831218-24831237 | MS.gene070467:intron | 45.0% |
| ! | GAATTTTCGCGTATCGAAGG+TGG | - | chr3.3:24831216-24831235 | MS.gene070467:intron | 45.0% |
| ! | GAGAGGATCGACACCAATTT+TGG | + | chr3.3:24832013-24832032 | None:intergenic | 45.0% |
| ! | GCTGAATTTTCGCGTATCGA+AGG | - | chr3.3:24831213-24831232 | MS.gene070467:intron | 45.0% |
| ! | GGGAGTTCTGACAAACATCT+TGG | - | chr3.3:24831399-24831418 | MS.gene070467:CDS | 45.0% |
| !! | GCCTGTTTCTACTGTTGCTT+CGG | - | chr3.3:24831902-24831921 | MS.gene070467:CDS | 45.0% |
| GACATCTCTTCAGTCAGCTG+TGG | + | chr3.3:24832205-24832224 | None:intergenic | 50.0% | |
| !! | TCGGAGTCTGCTTTTAGCAC+CGG | - | chr3.3:24831921-24831940 | MS.gene070467:CDS | 50.0% |
| !! | GAGTCTGCTTTTAGCACCGG+AGG | - | chr3.3:24831924-24831943 | MS.gene070467:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 24831156 | 24832243 | 24831156 | ID=MS.gene070467 |
| chr3.3 | mRNA | 24831156 | 24832243 | 24831156 | ID=MS.gene070467.t1;Parent=MS.gene070467 |
| chr3.3 | exon | 24831331 | 24832243 | 24831331 | ID=MS.gene070467.t1.exon1;Parent=MS.gene070467.t1 |
| chr3.3 | CDS | 24831331 | 24832243 | 24831331 | ID=cds.MS.gene070467.t1;Parent=MS.gene070467.t1 |
| chr3.3 | exon | 24831156 | 24831211 | 24831156 | ID=MS.gene070467.t1.exon2;Parent=MS.gene070467.t1 |
| chr3.3 | CDS | 24831156 | 24831211 | 24831156 | ID=cds.MS.gene070467.t1;Parent=MS.gene070467.t1 |
| Gene Sequence |
| Protein sequence |
