Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051575.t1 | XP_013465885.1 | 96.6 | 119 | 4 | 0 | 1 | 119 | 1 | 119 | 1.90E-58 | 235 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051575.t1 | A0A072VEC4 | 96.6 | 119 | 4 | 0 | 1 | 119 | 1 | 119 | 1.3e-58 | 235.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051575 | MS.gene06365 | 0.801957 | 7.04E-49 | -1.69E-46 |
| MS.gene051575 | MS.gene066313 | 0.800571 | 1.35E-48 | -1.69E-46 |
| MS.gene051575 | MS.gene06633 | 0.820184 | 7.84E-53 | -1.69E-46 |
| MS.gene051575 | MS.gene068494 | 0.812461 | 4.19E-51 | -1.69E-46 |
| MS.gene051575 | MS.gene069275 | 0.822702 | 2.06E-53 | -1.69E-46 |
| MS.gene051575 | MS.gene070467 | 0.803513 | 3.36E-49 | -1.69E-46 |
| MS.gene051575 | MS.gene072915 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene051575 | MS.gene20657 | 0.82387 | 1.10E-53 | -1.69E-46 |
| MS.gene051575 | MS.gene29008 | 0.802052 | 6.74E-49 | -1.69E-46 |
| MS.gene051575 | MS.gene30580 | 0.821056 | 4.95E-53 | -1.69E-46 |
| MS.gene051575 | MS.gene34615 | 0.801138 | 1.04E-48 | -1.69E-46 |
| MS.gene051575 | MS.gene38850 | 0.801425 | 9.06E-49 | -1.69E-46 |
| MS.gene051575 | MS.gene47552 | 0.822048 | 2.92E-53 | -1.69E-46 |
| MS.gene051575 | MS.gene54921 | 0.811823 | 5.77E-51 | -1.69E-46 |
| MS.gene051575 | MS.gene65003 | 0.821973 | 3.04E-53 | -1.69E-46 |
| MS.gene051575 | MS.gene73540 | 0.805092 | 1.58E-49 | -1.69E-46 |
| MS.gene051575 | MS.gene74758 | 0.801355 | 9.36E-49 | -1.69E-46 |
| MS.gene051575 | MS.gene82682 | 0.805268 | 1.45E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051575.t1 | MTR_1g015810 | 96.639 | 119 | 4 | 0 | 1 | 119 | 1 | 119 | 1.06e-67 | 199 |
| MS.gene051575.t1 | MTR_3g104640 | 67.241 | 116 | 30 | 5 | 1 | 116 | 1 | 108 | 1.05e-30 | 105 |
| MS.gene051575.t1 | MTR_4g125990 | 54.264 | 129 | 38 | 9 | 1 | 116 | 1 | 121 | 2.92e-27 | 97.8 |
| MS.gene051575.t1 | MTR_2g099630 | 50.000 | 90 | 37 | 3 | 31 | 119 | 26 | 108 | 6.67e-22 | 83.6 |
| MS.gene051575.t1 | MTR_8g018320 | 51.899 | 79 | 32 | 3 | 31 | 109 | 26 | 98 | 3.08e-16 | 68.9 |
| MS.gene051575.t1 | MTR_8g018360 | 48.235 | 85 | 38 | 3 | 31 | 115 | 26 | 104 | 1.56e-15 | 69.3 |
| MS.gene051575.t1 | MTR_7g109290 | 41.667 | 60 | 34 | 1 | 58 | 117 | 32 | 90 | 6.30e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051575.t1 | AT5G11970 | 54.622 | 119 | 40 | 7 | 1 | 119 | 1 | 105 | 2.77e-27 | 97.1 |
| MS.gene051575.t1 | AT3G13910 | 51.852 | 81 | 35 | 3 | 30 | 109 | 20 | 97 | 3.76e-16 | 68.6 |
| MS.gene051575.t1 | AT2G19460 | 43.333 | 120 | 48 | 5 | 1 | 110 | 1 | 110 | 8.20e-16 | 68.2 |
| MS.gene051575.t1 | AT3G13910 | 51.852 | 81 | 35 | 3 | 30 | 109 | 20 | 97 | 8.54e-16 | 68.2 |
| MS.gene051575.t1 | AT2G19460 | 43.333 | 120 | 48 | 5 | 1 | 110 | 53 | 162 | 1.70e-15 | 68.6 |
| MS.gene051575.t1 | AT1G72720 | 43.158 | 95 | 48 | 2 | 27 | 119 | 37 | 127 | 1.61e-13 | 62.8 |
| MS.gene051575.t1 | AT2G47480 | 45.161 | 62 | 32 | 1 | 57 | 116 | 47 | 108 | 1.29e-12 | 59.7 |
| MS.gene051575.t1 | AT3G05725 | 43.478 | 69 | 36 | 2 | 50 | 118 | 59 | 124 | 1.81e-11 | 57.4 |
| MS.gene051575.t1 | AT3G62640 | 52.381 | 42 | 20 | 0 | 75 | 116 | 68 | 109 | 1.89e-11 | 57.0 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTCAAGTCCTTATTATT+AGG | 0.268756 | 1.3:+6384432 | None:intergenic |
| GAAAAGTGAAAGGTTCATTT+AGG | 0.301962 | 1.3:-6384290 | MS.gene051575:CDS |
| TCAAAGGGGTCCTAATAATA+AGG | 0.325607 | 1.3:-6384442 | MS.gene051575:CDS |
| TAAGACCTAATATCATAATT+AGG | 0.344095 | 1.3:+6384489 | None:intergenic |
| ATTTCCAAGTCTTGGAGCTT+TGG | 0.345743 | 1.3:-6384375 | MS.gene051575:CDS |
| CTAAGCCTAATTATGATATT+AGG | 0.367646 | 1.3:-6384494 | MS.gene051575:CDS |
| CTGAATTTCAAAGAAAGAAA+AGG | 0.382930 | 1.3:-6384344 | MS.gene051575:CDS |
| GGTCTTCAATTTCCAAGTCT+TGG | 0.385601 | 1.3:-6384383 | MS.gene051575:CDS |
| AGGATTACAGCAAACAAATT+AGG | 0.410868 | 1.3:-6384566 | MS.gene051575:CDS |
| TTCCTATGCACAAACTCAAA+GGG | 0.443805 | 1.3:-6384457 | MS.gene051575:CDS |
| AGCAAACAAATTAGGCCCTA+TGG | 0.447501 | 1.3:-6384558 | MS.gene051575:CDS |
| AGTGGCTAAAGAATAGGTAT+TGG | 0.458659 | 1.3:-6384257 | MS.gene051575:CDS |
| TGAATTTCAAAGAAAGAAAA+GGG | 0.462597 | 1.3:-6384343 | MS.gene051575:CDS |
| GCTTTAAGTGGCTAAAGAAT+AGG | 0.471892 | 1.3:-6384263 | MS.gene051575:CDS |
| CATCATTTGACTATTTCCAT+AGG | 0.472718 | 1.3:+6384542 | None:intergenic |
| ATCACCAAAGCTCCAAGACT+TGG | 0.492773 | 1.3:+6384371 | None:intergenic |
| AGGACTTGAAGTTGAAGAAA+GGG | 0.493735 | 1.3:-6384422 | MS.gene051575:CDS |
| CATTTAGGAAAAGCTTTAAG+TGG | 0.499586 | 1.3:-6384275 | MS.gene051575:CDS |
| AAGGACTTGAAGTTGAAGAA+AGG | 0.504271 | 1.3:-6384423 | MS.gene051575:CDS |
| TACAAAATGTATTCTGTGGA+AGG | 0.507359 | 1.3:-6384312 | MS.gene051575:CDS |
| ATCATTTGACTATTTCCATA+GGG | 0.507995 | 1.3:+6384543 | None:intergenic |
| TTGTTTGCTGTAATCCTCCA+TGG | 0.512879 | 1.3:+6384572 | None:intergenic |
| TTTCCTATGCACAAACTCAA+AGG | 0.521927 | 1.3:-6384458 | MS.gene051575:CDS |
| GGCATGTGGTTTATAGCTTG+TGG | 0.550765 | 1.3:-6384236 | MS.gene051575:CDS |
| ATGCAGATGGAGAGCTACAA+TGG | 0.572922 | 1.3:-6384522 | MS.gene051575:CDS |
| AAATAGTCAAATGATGCAGA+TGG | 0.594245 | 1.3:-6384535 | MS.gene051575:CDS |
| AAGGGAAAAGCATTTCATCA+AGG | 0.598189 | 1.3:-6384404 | MS.gene051575:CDS |
| TCTGTGGAAGGAAAAGTGAA+AGG | 0.617851 | 1.3:-6384300 | MS.gene051575:CDS |
| AAAGAATAGGTATTGGCATG+TGG | 0.618847 | 1.3:-6384250 | MS.gene051575:CDS |
| TAGCTACAAAATGTATTCTG+TGG | 0.631804 | 1.3:-6384316 | MS.gene051575:CDS |
| ACCCCTTTGAGTTTGTGCAT+AGG | 0.638318 | 1.3:+6384455 | None:intergenic |
| TCCTATGCACAAACTCAAAG+GGG | 0.640633 | 1.3:-6384456 | MS.gene051575:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGACCTAATATCATAATT+AGG | + | chr1.3:6384317-6384336 | None:intergenic | 20.0% |
| !! | TGAATTTCAAAGAAAGAAAA+GGG | - | chr1.3:6384460-6384479 | MS.gene051575:CDS | 20.0% |
| ! | AACTTCAAGTCCTTATTATT+AGG | + | chr1.3:6384374-6384393 | None:intergenic | 25.0% |
| ! | ATCATTTGACTATTTCCATA+GGG | + | chr1.3:6384263-6384282 | None:intergenic | 25.0% |
| ! | CTAAGCCTAATTATGATATT+AGG | - | chr1.3:6384309-6384328 | MS.gene051575:CDS | 25.0% |
| ! | CTGAATTTCAAAGAAAGAAA+AGG | - | chr1.3:6384459-6384478 | MS.gene051575:CDS | 25.0% |
| !!! | CTTTTCTTTCTTTGAAATTC+AGG | + | chr1.3:6384461-6384480 | None:intergenic | 25.0% |
| AAATAGTCAAATGATGCAGA+TGG | - | chr1.3:6384268-6384287 | MS.gene051575:CDS | 30.0% | |
| AGGATTACAGCAAACAAATT+AGG | - | chr1.3:6384237-6384256 | MS.gene051575:CDS | 30.0% | |
| CATCATTTGACTATTTCCAT+AGG | + | chr1.3:6384264-6384283 | None:intergenic | 30.0% | |
| GAAAAGTGAAAGGTTCATTT+AGG | - | chr1.3:6384513-6384532 | MS.gene051575:CDS | 30.0% | |
| TACAAAATGTATTCTGTGGA+AGG | - | chr1.3:6384491-6384510 | MS.gene051575:CDS | 30.0% | |
| TAGCTACAAAATGTATTCTG+TGG | - | chr1.3:6384487-6384506 | MS.gene051575:CDS | 30.0% | |
| !! | CATTTAGGAAAAGCTTTAAG+TGG | - | chr1.3:6384528-6384547 | MS.gene051575:CDS | 30.0% |
| AAGGACTTGAAGTTGAAGAA+AGG | - | chr1.3:6384380-6384399 | MS.gene051575:CDS | 35.0% | |
| AGGACTTGAAGTTGAAGAAA+GGG | - | chr1.3:6384381-6384400 | MS.gene051575:CDS | 35.0% | |
| AGTGGCTAAAGAATAGGTAT+TGG | - | chr1.3:6384546-6384565 | MS.gene051575:CDS | 35.0% | |
| TCAAAGGGGTCCTAATAATA+AGG | - | chr1.3:6384361-6384380 | MS.gene051575:CDS | 35.0% | |
| TTCCTATGCACAAACTCAAA+GGG | - | chr1.3:6384346-6384365 | MS.gene051575:CDS | 35.0% | |
| TTTCCTATGCACAAACTCAA+AGG | - | chr1.3:6384345-6384364 | MS.gene051575:CDS | 35.0% | |
| ! | GCTTTAAGTGGCTAAAGAAT+AGG | - | chr1.3:6384540-6384559 | MS.gene051575:CDS | 35.0% |
| !! | AAAGAATAGGTATTGGCATG+TGG | - | chr1.3:6384553-6384572 | MS.gene051575:CDS | 35.0% |
| !! | AAGGGAAAAGCATTTCATCA+AGG | - | chr1.3:6384399-6384418 | MS.gene051575:CDS | 35.0% |
| AGCAAACAAATTAGGCCCTA+TGG | - | chr1.3:6384245-6384264 | MS.gene051575:CDS | 40.0% | |
| ATTTCCAAGTCTTGGAGCTT+TGG | - | chr1.3:6384428-6384447 | MS.gene051575:CDS | 40.0% | |
| GGTCTTCAATTTCCAAGTCT+TGG | - | chr1.3:6384420-6384439 | MS.gene051575:CDS | 40.0% | |
| TCCTATGCACAAACTCAAAG+GGG | - | chr1.3:6384347-6384366 | MS.gene051575:CDS | 40.0% | |
| TCTGTGGAAGGAAAAGTGAA+AGG | - | chr1.3:6384503-6384522 | MS.gene051575:CDS | 40.0% | |
| ATCACCAAAGCTCCAAGACT+TGG | + | chr1.3:6384435-6384454 | None:intergenic | 45.0% | |
| ATGCAGATGGAGAGCTACAA+TGG | - | chr1.3:6384281-6384300 | MS.gene051575:CDS | 45.0% | |
| GGCATGTGGTTTATAGCTTG+TGG | - | chr1.3:6384567-6384586 | MS.gene051575:CDS | 45.0% | |
| ! | ACCCCTTTGAGTTTGTGCAT+AGG | + | chr1.3:6384351-6384370 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 6384233 | 6384592 | 6384233 | ID=MS.gene051575 |
| chr1.3 | mRNA | 6384233 | 6384592 | 6384233 | ID=MS.gene051575.t1;Parent=MS.gene051575 |
| chr1.3 | exon | 6384233 | 6384592 | 6384233 | ID=MS.gene051575.t1.exon1;Parent=MS.gene051575.t1 |
| chr1.3 | CDS | 6384233 | 6384592 | 6384233 | ID=cds.MS.gene051575.t1;Parent=MS.gene051575.t1 |
| Gene Sequence |
| Protein sequence |
