Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070979.t1 | AAK63815.1 | 100 | 199 | 0 | 0 | 1 | 199 | 1 | 199 | 1.90E-87 | 332 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070979.t1 | P11432 | 76.4 | 199 | 44 | 1 | 1 | 199 | 1 | 196 | 2.6e-74 | 279.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070979.t1 | Q94F86 | 100.0 | 199 | 0 | 0 | 1 | 199 | 1 | 199 | 1.3e-87 | 332.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049369 | MS.gene070979 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene051734 | MS.gene070979 | 0.813805 | 2.13E-51 | -1.69E-46 |
| MS.gene052469 | MS.gene070979 | 0.845178 | 4.78E-59 | -1.69E-46 |
| MS.gene052870 | MS.gene070979 | 0.841848 | 3.70E-58 | -1.69E-46 |
| MS.gene052872 | MS.gene070979 | 0.838502 | 2.77E-57 | -1.69E-46 |
| MS.gene053824 | MS.gene070979 | 0.810051 | 1.40E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene070979 | 0.827592 | 1.44E-54 | -1.69E-46 |
| MS.gene055224 | MS.gene070979 | 0.800809 | 1.21E-48 | -1.69E-46 |
| MS.gene055539 | MS.gene070979 | 0.823107 | 1.66E-53 | -1.69E-46 |
| MS.gene055741 | MS.gene070979 | 0.80979 | 1.59E-50 | -1.69E-46 |
| MS.gene055744 | MS.gene070979 | 0.809412 | 1.92E-50 | -1.69E-46 |
| MS.gene058448 | MS.gene070979 | 0.80454 | 2.06E-49 | -1.69E-46 |
| MS.gene059717 | MS.gene070979 | 0.851326 | 9.55E-61 | -1.69E-46 |
| MS.gene060832 | MS.gene070979 | 0.809037 | 2.31E-50 | -1.69E-46 |
| MS.gene060981 | MS.gene070979 | 0.810432 | 1.16E-50 | -1.69E-46 |
| MS.gene061231 | MS.gene070979 | 0.812294 | 4.56E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070979.t1 | MTR_1g102780 | 97.000 | 200 | 5 | 1 | 1 | 199 | 1 | 200 | 8.21e-138 | 383 |
| MS.gene070979.t1 | MTR_1g102800 | 91.960 | 199 | 14 | 1 | 1 | 199 | 1 | 197 | 5.56e-111 | 315 |
| MS.gene070979.t1 | MTR_1g102570 | 88.889 | 180 | 18 | 1 | 20 | 199 | 21 | 198 | 5.10e-110 | 313 |
| MS.gene070979.t1 | MTR_1g102830 | 82.222 | 180 | 18 | 2 | 20 | 199 | 21 | 186 | 3.16e-102 | 293 |
| MS.gene070979.t1 | MTR_1g102810 | 78.689 | 183 | 25 | 2 | 17 | 199 | 14 | 182 | 1.57e-99 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070979.t1 | AT3G22840 | 53.886 | 193 | 83 | 3 | 7 | 199 | 9 | 195 | 8.74e-61 | 188 |
| MS.gene070979.t1 | AT4G14690 | 56.647 | 173 | 72 | 2 | 28 | 199 | 23 | 193 | 3.22e-57 | 179 |
Find 57 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAAGGGATGTTGTTAAAT+TGG | 0.214729 | 1.4:+79313995 | None:intergenic |
| GGTGCTGATGGTGGTGTAAT+TGG | 0.245963 | 1.4:+79313713 | None:intergenic |
| TGGTGGAATTCCATGGTTCT+TGG | 0.288858 | 1.4:-79313403 | MS.gene070979:CDS |
| GGTGGAATTCCATGGTTCTT+GGG | 0.299911 | 1.4:-79313402 | MS.gene070979:CDS |
| GGTTTGTTTGATCAAATATC+TGG | 0.314167 | 1.4:-79313426 | MS.gene070979:CDS |
| GTTGGTTGTGGTTGTGGTGG+TGG | 0.344690 | 1.4:+79313686 | None:intergenic |
| TGTGGTTGTGGTGGTGGAGT+TGG | 0.352890 | 1.4:+79313692 | None:intergenic |
| ATTGGGTCTACAGGCACCTT+TGG | 0.360861 | 1.4:+79313731 | None:intergenic |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1.4:-79313262 | MS.gene070979:CDS |
| AATGGAAGGTTGGCTATGAT+TGG | 0.393839 | 1.4:-79313489 | MS.gene070979:CDS |
| CCATTGATTCTCTCAGGTGC+TGG | 0.411549 | 1.4:+79313507 | None:intergenic |
| GGTGGTGGAGTTGGTGCTGA+TGG | 0.417357 | 1.4:+79313701 | None:intergenic |
| CACCTTTGGTGAACGAGTAT+AGG | 0.424707 | 1.4:+79313664 | None:intergenic |
| AACCTTCCATTGATTCTCTC+AGG | 0.436061 | 1.4:+79313501 | None:intergenic |
| GTGCTGATGGTGGTGTAATT+GGG | 0.440700 | 1.4:+79313714 | None:intergenic |
| GAATGGTAGAATTGCTATGT+TGG | 0.450278 | 1.4:-79313277 | MS.gene070979:CDS |
| TCTAGGCCTGCTTGAAATGC+TGG | 0.450968 | 1.4:+79314023 | None:intergenic |
| GTATAGGTTGGTTGTGGTTG+TGG | 0.452125 | 1.4:+79313680 | None:intergenic |
| TTGTTAAATTGGTTAACTCT+AGG | 0.463045 | 1.4:+79314006 | None:intergenic |
| GTGGAATTCCATGGTTCTTG+GGG | 0.468120 | 1.4:-79313401 | MS.gene070979:CDS |
| GGTGGAGTTGGTGCTGATGG+TGG | 0.468618 | 1.4:+79313704 | None:intergenic |
| CGGATTTGATGGCATTTGGT+GGG | 0.485102 | 1.4:-79313530 | MS.gene070979:CDS |
| TCGGATTTGATGGCATTTGG+TGG | 0.487791 | 1.4:-79313531 | MS.gene070979:CDS |
| AATGGTAGAATTGCTATGTT+GGG | 0.492247 | 1.4:-79313276 | MS.gene070979:CDS |
| GGAATGCTAGCCTCAAAGTT+CGG | 0.497529 | 1.4:-79313951 | MS.gene070979:CDS |
| GGTGGTGTAATTGGGTCTAC+AGG | 0.516474 | 1.4:+79313722 | None:intergenic |
| CCAGCACCTGAGAGAATCAA+TGG | 0.516939 | 1.4:-79313507 | MS.gene070979:CDS |
| TAGGTTGGTTGTGGTTGTGG+TGG | 0.523841 | 1.4:+79313683 | None:intergenic |
| AGTGTGGAGTCTAAGTCAAA+AGG | 0.525000 | 1.4:-79313327 | MS.gene070979:CDS |
| CAGCCATGGACCGAACTTTG+AGG | 0.527132 | 1.4:+79313941 | None:intergenic |
| CGAAAACATTACCTCAGCCA+TGG | 0.528935 | 1.4:+79313927 | None:intergenic |
| CAAAGTTCGGTCCATGGCTG+AGG | 0.533870 | 1.4:-79313938 | MS.gene070979:intron |
| CTAGCATTCCTTCTGAAGCT+AGG | 0.541676 | 1.4:+79313964 | None:intergenic |
| GGTGGAGATAGCAAATGGAC+AGG | 0.547189 | 1.4:-79313448 | MS.gene070979:CDS |
| CCTGCTTGAAATGCTGGTCA+TGG | 0.548863 | 1.4:+79314029 | None:intergenic |
| TTTGATCAAATATCTGGTGG+TGG | 0.559193 | 1.4:-79313420 | MS.gene070979:CDS |
| TGAGAGAATCAATGGAAGGT+TGG | 0.560259 | 1.4:-79313499 | MS.gene070979:CDS |
| GAACGAGTATAGGTTGGTTG+TGG | 0.560878 | 1.4:+79313674 | None:intergenic |
| TTGTTTGATCAAATATCTGG+TGG | 0.570609 | 1.4:-79313423 | MS.gene070979:CDS |
| TAGCCTCAAAGTTCGGTCCA+TGG | 0.581082 | 1.4:-79313944 | MS.gene070979:CDS |
| TTTGGTGAACGAGTATAGGT+TGG | 0.589396 | 1.4:+79313668 | None:intergenic |
| AAGCTAGGCATATACACAGA+AGG | 0.589450 | 1.4:+79313979 | None:intergenic |
| CTATAGAAATCTATTACACA+AGG | 0.595515 | 1.4:+79313211 | None:intergenic |
| AACCTATACTCGTTCACCAA+AGG | 0.597244 | 1.4:-79313666 | MS.gene070979:intron |
| TAGACTCCACACTAACACCT+TGG | 0.597870 | 1.4:+79313337 | None:intergenic |
| TGTCCTCAGATGCTGAGTTG+TGG | 0.599794 | 1.4:-79313299 | MS.gene070979:CDS |
| TGTATATGCCTAGCTTCAGA+AGG | 0.600911 | 1.4:-79313972 | MS.gene070979:CDS |
| CCATGACCAGCATTTCAAGC+AGG | 0.603962 | 1.4:-79314029 | MS.gene070979:CDS |
| ATTCCACAACTCAGCATCTG+AGG | 0.606044 | 1.4:+79313296 | None:intergenic |
| AGCTAGGCATATACACAGAA+GGG | 0.613758 | 1.4:+79313980 | None:intergenic |
| TATCTGGTGGTGGAATTCCA+TGG | 0.624337 | 1.4:-79313410 | MS.gene070979:CDS |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.4:-79313747 | MS.gene070979:CDS |
| ACACTTGTCCCCAAGAACCA+TGG | 0.633615 | 1.4:+79313393 | None:intergenic |
| TCAGATGCTGAGTTGTGGAA+TGG | 0.641735 | 1.4:-79313294 | MS.gene070979:CDS |
| GTGGAGATAGCAAATGGACA+GGG | 0.645295 | 1.4:-79313447 | MS.gene070979:CDS |
| CACCTGAGAGAATCAATGGA+AGG | 0.646646 | 1.4:-79313503 | MS.gene070979:CDS |
| TATAGAAATCTATTACACAA+GGG | 0.717921 | 1.4:+79313212 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAATAATGATGAAAATA+TGG | - | chr1.4:79313718-79313737 | MS.gene070979:CDS | 15.0% |
| !!! | AGTTTTTCATTACATTTATC+AGG | - | chr1.4:79313519-79313538 | MS.gene070979:CDS | 20.0% |
| ! | TTCATTACATTTATCAGGAA+GGG | - | chr1.4:79313524-79313543 | MS.gene070979:CDS | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | + | chr1.4:79313287-79313306 | None:intergenic | 25.0% |
| ! | TTTCATTACATTTATCAGGA+AGG | - | chr1.4:79313523-79313542 | MS.gene070979:CDS | 25.0% |
| !! | GCTTTTACAGAATATGTTAA+GGG | - | chr1.4:79314050-79314069 | MS.gene070979:CDS | 25.0% |
| !! | TGTTTTCGAAAACAGATTTA+AGG | - | chr1.4:79313378-79313397 | MS.gene070979:CDS | 25.0% |
| AATGGTAGAATTGCTATGTT+GGG | - | chr1.4:79314014-79314033 | MS.gene070979:CDS | 30.0% | |
| TGCATAATGCAACAAGTTAA+TGG | - | chr1.4:79313434-79313453 | MS.gene070979:CDS | 30.0% | |
| TTGTTTGATCAAATATCTGG+TGG | - | chr1.4:79313867-79313886 | MS.gene070979:intron | 30.0% | |
| ! | GGCTTTTACAGAATATGTTA+AGG | - | chr1.4:79314049-79314068 | MS.gene070979:CDS | 30.0% |
| ! | GGTTTGTTTGATCAAATATC+TGG | - | chr1.4:79313864-79313883 | MS.gene070979:intron | 30.0% |
| !! | TCTTTGATTCCATTTTTCCA+AGG | - | chr1.4:79313936-79313955 | MS.gene070979:intron | 30.0% |
| ACACTAACACCTTGGAAAAA+TGG | + | chr1.4:79313948-79313967 | None:intergenic | 35.0% | |
| CAGAAGGGATGTTGTTAAAT+TGG | + | chr1.4:79313298-79313317 | None:intergenic | 35.0% | |
| GAATGGTAGAATTGCTATGT+TGG | - | chr1.4:79314013-79314032 | MS.gene070979:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | - | chr1.4:79314028-79314047 | MS.gene070979:CDS | 35.0% | |
| TTTACTACTATGCTCACCTT+TGG | + | chr1.4:79313643-79313662 | None:intergenic | 35.0% | |
| TTTGATCAAATATCTGGTGG+TGG | - | chr1.4:79313870-79313889 | MS.gene070979:intron | 35.0% | |
| ! | CACAAAGTTTTCGGATTTGA+TGG | - | chr1.4:79313749-79313768 | MS.gene070979:CDS | 35.0% |
| !! | ATTTTTCCAAGGTGTTAGTG+TGG | - | chr1.4:79313947-79313966 | MS.gene070979:CDS | 35.0% |
| !! | TTTTCGGATTTGATGGCATT+TGG | - | chr1.4:79313756-79313775 | MS.gene070979:intron | 35.0% |
| !!! | ATTGGTTTTGTAGCTGCTAT+GGG | - | chr1.4:79313819-79313838 | MS.gene070979:intron | 35.0% |
| AACCTATACTCGTTCACCAA+AGG | - | chr1.4:79313624-79313643 | MS.gene070979:intron | 40.0% | |
| AAGCTAGGCATATACACAGA+AGG | + | chr1.4:79313314-79313333 | None:intergenic | 40.0% | |
| AGCTAGGCATATACACAGAA+GGG | + | chr1.4:79313313-79313332 | None:intergenic | 40.0% | |
| AGTGTGGAGTCTAAGTCAAA+AGG | - | chr1.4:79313963-79313982 | MS.gene070979:CDS | 40.0% | |
| TGAGAGAATCAATGGAAGGT+TGG | - | chr1.4:79313791-79313810 | MS.gene070979:intron | 40.0% | |
| TGTATATGCCTAGCTTCAGA+AGG | - | chr1.4:79313318-79313337 | MS.gene070979:CDS | 40.0% | |
| TTTGGTGAACGAGTATAGGT+TGG | + | chr1.4:79313625-79313644 | None:intergenic | 40.0% | |
| ! | AATGGAAGGTTGGCTATGAT+TGG | - | chr1.4:79313801-79313820 | MS.gene070979:intron | 40.0% |
| ! | GCAGATGAGCACAAAGTTTT+CGG | - | chr1.4:79313740-79313759 | MS.gene070979:CDS | 40.0% |
| !! | AACCTTCCATTGATTCTCTC+AGG | + | chr1.4:79313792-79313811 | None:intergenic | 40.0% |
| !!! | GATTGGTTTTGTAGCTGCTA+TGG | - | chr1.4:79313818-79313837 | MS.gene070979:intron | 40.0% |
| !!! | TTGGTTTTGTAGCTGCTATG+GGG | - | chr1.4:79313820-79313839 | MS.gene070979:intron | 40.0% |
| ATTCCACAACTCAGCATCTG+AGG | + | chr1.4:79313997-79314016 | None:intergenic | 45.0% | |
| CACCTGAGAGAATCAATGGA+AGG | - | chr1.4:79313787-79313806 | MS.gene070979:intron | 45.0% | |
| CACCTTTGGTGAACGAGTAT+AGG | + | chr1.4:79313629-79313648 | None:intergenic | 45.0% | |
| CGAAAACATTACCTCAGCCA+TGG | + | chr1.4:79313366-79313385 | None:intergenic | 45.0% | |
| GGTGGAATTCCATGGTTCTT+GGG | - | chr1.4:79313888-79313907 | MS.gene070979:intron | 45.0% | |
| GTGGAATTCCATGGTTCTTG+GGG | - | chr1.4:79313889-79313908 | MS.gene070979:intron | 45.0% | |
| GTGGAGATAGCAAATGGACA+GGG | - | chr1.4:79313843-79313862 | MS.gene070979:intron | 45.0% | |
| TAGACTCCACACTAACACCT+TGG | + | chr1.4:79313956-79313975 | None:intergenic | 45.0% | |
| TCAGATGCTGAGTTGTGGAA+TGG | - | chr1.4:79313996-79314015 | MS.gene070979:CDS | 45.0% | |
| TGGTGGAATTCCATGGTTCT+TGG | - | chr1.4:79313887-79313906 | MS.gene070979:intron | 45.0% | |
| ! | CGGATTTGATGGCATTTGGT+GGG | - | chr1.4:79313760-79313779 | MS.gene070979:intron | 45.0% |
| ! | GGAATGCTAGCCTCAAAGTT+CGG | - | chr1.4:79313339-79313358 | MS.gene070979:CDS | 45.0% |
| ! | TCGGATTTGATGGCATTTGG+TGG | - | chr1.4:79313759-79313778 | MS.gene070979:intron | 45.0% |
| !! | CTAGCATTCCTTCTGAAGCT+AGG | + | chr1.4:79313329-79313348 | None:intergenic | 45.0% |
| !! | GAACGAGTATAGGTTGGTTG+TGG | + | chr1.4:79313619-79313638 | None:intergenic | 45.0% |
| !! | GTATAGGTTGGTTGTGGTTG+TGG | + | chr1.4:79313613-79313632 | None:intergenic | 45.0% |
| !! | GTGCTGATGGTGGTGTAATT+GGG | + | chr1.4:79313579-79313598 | None:intergenic | 45.0% |
| !! | TATCTGGTGGTGGAATTCCA+TGG | - | chr1.4:79313880-79313899 | MS.gene070979:intron | 45.0% |
| !!! | TGGTTTTGTAGCTGCTATGG+GGG | - | chr1.4:79313821-79313840 | MS.gene070979:intron | 45.0% |
| ACACTTGTCCCCAAGAACCA+TGG | + | chr1.4:79313900-79313919 | None:intergenic | 50.0% | |
| AGGGCAGAAAGAGCAACCAA+AGG | - | chr1.4:79313543-79313562 | MS.gene070979:CDS | 50.0% | |
| ATGGGGGTGGAGATAGCAAA+TGG | - | chr1.4:79313837-79313856 | MS.gene070979:intron | 50.0% | |
| ATTGGGTCTACAGGCACCTT+TGG | + | chr1.4:79313562-79313581 | None:intergenic | 50.0% | |
| CCAGCACCTGAGAGAATCAA+TGG | - | chr1.4:79313783-79313802 | MS.gene070979:intron | 50.0% | |
| CCATGACCAGCATTTCAAGC+AGG | - | chr1.4:79313261-79313280 | MS.gene070979:CDS | 50.0% | |
| CCTGCTTGAAATGCTGGTCA+TGG | + | chr1.4:79313264-79313283 | None:intergenic | 50.0% | |
| GGTGGAGATAGCAAATGGAC+AGG | - | chr1.4:79313842-79313861 | MS.gene070979:intron | 50.0% | |
| GGTGGTGTAATTGGGTCTAC+AGG | + | chr1.4:79313571-79313590 | None:intergenic | 50.0% | |
| TAGCCTCAAAGTTCGGTCCA+TGG | - | chr1.4:79313346-79313365 | MS.gene070979:CDS | 50.0% | |
| TCTAGGCCTGCTTGAAATGC+TGG | + | chr1.4:79313270-79313289 | None:intergenic | 50.0% | |
| TGTCCTCAGATGCTGAGTTG+TGG | - | chr1.4:79313991-79314010 | MS.gene070979:CDS | 50.0% | |
| ! | TTTTGTAGCTGCTATGGGGG+TGG | - | chr1.4:79313824-79313843 | MS.gene070979:intron | 50.0% |
| !! | CCATTGATTCTCTCAGGTGC+TGG | + | chr1.4:79313786-79313805 | None:intergenic | 50.0% |
| !! | GGTGCTGATGGTGGTGTAAT+TGG | + | chr1.4:79313580-79313599 | None:intergenic | 50.0% |
| !! | TAGGTTGGTTGTGGTTGTGG+TGG | + | chr1.4:79313610-79313629 | None:intergenic | 50.0% |
| CAAAGTTCGGTCCATGGCTG+AGG | - | chr1.4:79313352-79313371 | MS.gene070979:CDS | 55.0% | |
| CAGCCATGGACCGAACTTTG+AGG | + | chr1.4:79313352-79313371 | None:intergenic | 55.0% | |
| GTTGGTTGTGGTTGTGGTGG+TGG | + | chr1.4:79313607-79313626 | None:intergenic | 55.0% | |
| !! | TGTGGTTGTGGTGGTGGAGT+TGG | + | chr1.4:79313601-79313620 | None:intergenic | 55.0% |
| !! | GGTGGAGTTGGTGCTGATGG+TGG | + | chr1.4:79313589-79313608 | None:intergenic | 60.0% |
| !! | GGTGGTGGAGTTGGTGCTGA+TGG | + | chr1.4:79313592-79313611 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 79313224 | 79314088 | 79313224 | ID=MS.gene070979 |
| chr1.4 | mRNA | 79313224 | 79314088 | 79313224 | ID=MS.gene070979.t1;Parent=MS.gene070979 |
| chr1.4 | exon | 79313939 | 79314088 | 79313939 | ID=MS.gene070979.t1.exon1;Parent=MS.gene070979.t1 |
| chr1.4 | CDS | 79313939 | 79314088 | 79313939 | ID=cds.MS.gene070979.t1;Parent=MS.gene070979.t1 |
| chr1.4 | exon | 79313667 | 79313771 | 79313667 | ID=MS.gene070979.t1.exon2;Parent=MS.gene070979.t1 |
| chr1.4 | CDS | 79313667 | 79313771 | 79313667 | ID=cds.MS.gene070979.t1;Parent=MS.gene070979.t1 |
| chr1.4 | exon | 79313224 | 79313568 | 79313224 | ID=MS.gene070979.t1.exon3;Parent=MS.gene070979.t1 |
| chr1.4 | CDS | 79313224 | 79313568 | 79313224 | ID=cds.MS.gene070979.t1;Parent=MS.gene070979.t1 |
| Gene Sequence |
| Protein sequence |
