Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071445.t1 | RHN79104.1 | 100 | 80 | 0 | 0 | 1 | 80 | 668 | 747 | 2.00E-40 | 174.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071445.t1 | O81644 | 72.5 | 80 | 20 | 1 | 1 | 80 | 667 | 744 | 2.6e-28 | 125.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071445.t1 | A0A396JPH0 | 100.0 | 80 | 0 | 0 | 1 | 80 | 668 | 747 | 1.4e-40 | 174.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051181 | MS.gene071445 | 0.803044 | 4.21E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene071445 | 0.816691 | 4.85E-52 | -1.69E-46 |
| MS.gene05414 | MS.gene071445 | 0.800963 | 1.13E-48 | -1.69E-46 |
| MS.gene054775 | MS.gene071445 | 0.806077 | 9.80E-50 | -1.69E-46 |
| MS.gene054801 | MS.gene071445 | 0.827802 | 1.28E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene071445 | 0.812417 | 4.28E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene071445 | 0.808984 | 2.37E-50 | -1.69E-46 |
| MS.gene056252 | MS.gene071445 | 0.801407 | 9.14E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene071445 | 0.836407 | 9.53E-57 | -1.69E-46 |
| MS.gene058979 | MS.gene071445 | 0.817866 | 2.64E-52 | -1.69E-46 |
| MS.gene059584 | MS.gene071445 | 0.810973 | 8.83E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071445.t1 | MTR_7g091460 | 75.000 | 80 | 18 | 1 | 1 | 80 | 671 | 748 | 1.23e-36 | 129 |
| MS.gene071445.t1 | MTR_3g463530 | 45.714 | 70 | 36 | 1 | 5 | 74 | 667 | 734 | 4.76e-15 | 68.6 |
| MS.gene071445.t1 | MTR_3g463530 | 45.714 | 70 | 36 | 1 | 5 | 74 | 667 | 734 | 4.99e-15 | 68.2 |
| MS.gene071445.t1 | MTR_4g127660 | 39.437 | 71 | 41 | 1 | 2 | 72 | 671 | 739 | 8.49e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.33e-35 | 126 |
| MS.gene071445.t1 | AT3G57410 | 72.500 | 80 | 20 | 1 | 1 | 80 | 669 | 746 | 2.64e-35 | 126 |
| MS.gene071445.t1 | AT2G41740 | 72.500 | 80 | 20 | 1 | 1 | 80 | 667 | 744 | 1.50e-34 | 124 |
| MS.gene071445.t1 | AT2G41740 | 72.500 | 80 | 20 | 1 | 1 | 80 | 667 | 744 | 1.50e-34 | 124 |
| MS.gene071445.t1 | AT3G57410 | 84.615 | 52 | 8 | 0 | 1 | 52 | 669 | 720 | 6.62e-27 | 102 |
| MS.gene071445.t1 | AT5G57320 | 42.308 | 78 | 43 | 1 | 2 | 79 | 671 | 746 | 1.59e-16 | 72.8 |
| MS.gene071445.t1 | AT5G57320 | 42.308 | 78 | 43 | 1 | 2 | 79 | 671 | 746 | 1.59e-16 | 72.8 |
| MS.gene071445.t1 | AT4G30160 | 43.590 | 78 | 43 | 1 | 2 | 79 | 678 | 754 | 3.63e-16 | 71.6 |
| MS.gene071445.t1 | AT4G30160 | 43.590 | 78 | 43 | 1 | 2 | 79 | 669 | 745 | 3.85e-16 | 71.6 |
| MS.gene071445.t1 | AT4G30160 | 43.590 | 78 | 43 | 1 | 2 | 79 | 669 | 745 | 3.85e-16 | 71.6 |
| MS.gene071445.t1 | AT4G30160 | 43.590 | 78 | 43 | 1 | 2 | 79 | 669 | 745 | 3.85e-16 | 71.6 |
| MS.gene071445.t1 | AT2G29890 | 44.286 | 70 | 37 | 1 | 5 | 74 | 671 | 738 | 1.14e-13 | 64.7 |
| MS.gene071445.t1 | AT2G29890 | 44.286 | 70 | 37 | 1 | 5 | 74 | 652 | 719 | 1.15e-13 | 64.7 |
| MS.gene071445.t1 | AT2G29890 | 44.286 | 70 | 37 | 1 | 5 | 74 | 669 | 736 | 1.17e-13 | 64.7 |
| MS.gene071445.t1 | AT2G29890 | 44.286 | 70 | 37 | 1 | 5 | 74 | 669 | 736 | 1.17e-13 | 64.7 |
| MS.gene071445.t1 | AT2G29890 | 44.286 | 70 | 37 | 1 | 5 | 74 | 671 | 738 | 1.17e-13 | 64.7 |
Find 19 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTCTGTTACTTTATATA+AGG | 0.143430 | 1.2:+27308415 | None:intergenic |
| CATAGATATGGCTGCATCTT+TGG | 0.219462 | 1.2:-27308459 | MS.gene071445:CDS |
| AGAGTAGAGACACCTGTTTC+TGG | 0.228660 | 1.2:+27307978 | None:intergenic |
| AAACAGGTGTCTCTACTCTT+TGG | 0.322037 | 1.2:-27307974 | MS.gene071445:CDS |
| AACAGGTGTCTCTACTCTTT+GGG | 0.351499 | 1.2:-27307973 | MS.gene071445:CDS |
| CCTTCTGTTACTTTATATAA+GGG | 0.389348 | 1.2:+27308416 | None:intergenic |
| ACTTCAGAAATACATAGATA+TGG | 0.393668 | 1.2:-27308471 | MS.gene071445:intron |
| TTCTTATTACTTCAGGTTCA+CGG | 0.422765 | 1.2:-27308010 | MS.gene071445:intron |
| CGGGAACTCATTCCAGAAAC+AGG | 0.435091 | 1.2:-27307990 | MS.gene071445:CDS |
| TCTTATTACTTCAGGTTCAC+GGG | 0.476574 | 1.2:-27308009 | MS.gene071445:intron |
| GTGTCTCTACTCTTTGGGAT+TGG | 0.491928 | 1.2:-27307968 | MS.gene071445:CDS |
| CTTTGGGATTGGCCATGCTG+TGG | 0.526752 | 1.2:-27307957 | MS.gene071445:CDS |
| GATATGGCTGCATCTTTGGA+AGG | 0.564349 | 1.2:-27308455 | MS.gene071445:CDS |
| CCCTTATATAAAGTAACAGA+AGG | 0.571998 | 1.2:-27308416 | MS.gene071445:CDS |
| TGGGATTGGCCATGCTGTGG+AGG | 0.583414 | 1.2:-27307954 | None:intergenic |
| TTGGGATTATACAAGAGCTA+AGG | 0.603992 | 1.2:-27308354 | MS.gene071445:intron |
| AGGACTGTACCTCCACAGCA+TGG | 0.675625 | 1.2:+27307945 | None:intergenic |
| ACTTTATATAAGGGTACACG+TGG | 0.687862 | 1.2:+27308425 | None:intergenic |
| CCTTATATAAAGTAACAGAA+GGG | 0.690283 | 1.2:-27308415 | MS.gene071445:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCTAAATGTTTTAAATGT+TGG | - | chr1.2:27308419-27308438 | MS.gene071445:CDS | 15.0% |
| !!! | TTTATTTTTCTTATTACTTC+AGG | - | chr1.2:27308527-27308546 | MS.gene071445:intron | 15.0% |
| !!! | TTTAATTTGTTGACTTTACA+TGG | - | chr1.2:27308504-27308523 | MS.gene071445:intron | 20.0% |
| ! | AAGTATGTAGTGAAAAAGAA+AGG | + | chr1.2:27308161-27308180 | None:intergenic | 25.0% |
| ! | ACTTCAGAAATACATAGATA+TGG | - | chr1.2:27308073-27308092 | MS.gene071445:intron | 25.0% |
| ! | CCTTATATAAAGTAACAGAA+GGG | - | chr1.2:27308129-27308148 | MS.gene071445:intron | 25.0% |
| ! | TATGTCCAAAGGAATTAAAA+TGG | - | chr1.2:27308213-27308232 | MS.gene071445:intron | 25.0% |
| !! | AGAATTGAATTTTACTTACC+TGG | + | chr1.2:27307999-27308018 | None:intergenic | 25.0% |
| !! | AGAATTTTGTCATCACATAT+AGG | - | chr1.2:27308382-27308401 | MS.gene071445:CDS | 25.0% |
| !! | TTTCACTACATACTTTTCAT+GGG | - | chr1.2:27308166-27308185 | MS.gene071445:intron | 25.0% |
| !! | TTTTCACTACATACTTTTCA+TGG | - | chr1.2:27308165-27308184 | MS.gene071445:intron | 25.0% |
| !!! | AAAGCATTTTGCTTTTCTTT+AGG | + | chr1.2:27307967-27307986 | None:intergenic | 25.0% |
| !!! | AAGCAAAATGCTTTTGAAAT+TGG | - | chr1.2:27307973-27307992 | MS.gene071445:CDS | 25.0% |
| !!! | AAGCATTTTGCTTTTCTTTA+GGG | + | chr1.2:27307966-27307985 | None:intergenic | 25.0% |
| !!! | CCTTCTGTTACTTTATATAA+GGG | + | chr1.2:27308131-27308150 | None:intergenic | 25.0% |
| !!! | TATTTCTGAAGTAAAGTTCA+AGG | + | chr1.2:27308065-27308084 | None:intergenic | 25.0% |
| AAACAACGAACAAACCAAAA+GGG | + | chr1.2:27308239-27308258 | None:intergenic | 30.0% | |
| ATGTGATGACAAAATTCTAG+TGG | + | chr1.2:27308380-27308399 | None:intergenic | 30.0% | |
| CCCTTATATAAAGTAACAGA+AGG | - | chr1.2:27308128-27308147 | MS.gene071445:intron | 30.0% | |
| TAAACAACGAACAAACCAAA+AGG | + | chr1.2:27308240-27308259 | None:intergenic | 30.0% | |
| TGTGATGACAAAATTCTAGT+GGG | + | chr1.2:27308379-27308398 | None:intergenic | 30.0% | |
| ! | AATATTTTGCATTTCTGTGC+TGG | - | chr1.2:27308312-27308331 | MS.gene071445:intron | 30.0% |
| ! | TACATACTTTTCATGGGATT+GGG | - | chr1.2:27308172-27308191 | MS.gene071445:intron | 30.0% |
| ! | TTCTTATTACTTCAGGTTCA+CGG | - | chr1.2:27308534-27308553 | MS.gene071445:intron | 30.0% |
| !! | CCCTTCTGTTACTTTATATA+AGG | + | chr1.2:27308132-27308151 | None:intergenic | 30.0% |
| ATAAATCAAGTACCCCAAGT+AGG | + | chr1.2:27308042-27308061 | None:intergenic | 35.0% | |
| GGTAAAATGATGTGATTGTG+AGG | - | chr1.2:27308460-27308479 | MS.gene071445:CDS | 35.0% | |
| TGAGCAGAACATAATTACCA+TGG | - | chr1.2:27308342-27308361 | MS.gene071445:intron | 35.0% | |
| TGGTCTAAAAAGATTGCTAG+TGG | - | chr1.2:27308439-27308458 | MS.gene071445:CDS | 35.0% | |
| TTGGGATTATACAAGAGCTA+AGG | - | chr1.2:27308190-27308209 | MS.gene071445:intron | 35.0% | |
| ! | ACTTTATATAAGGGTACACG+TGG | + | chr1.2:27308122-27308141 | None:intergenic | 35.0% |
| ! | AGGAATTAAAATGGCCCTTT+TGG | - | chr1.2:27308222-27308241 | MS.gene071445:intron | 35.0% |
| ! | CTACATACTTTTCATGGGAT+TGG | - | chr1.2:27308171-27308190 | MS.gene071445:intron | 35.0% |
| ! | TCTTATTACTTCAGGTTCAC+GGG | - | chr1.2:27308535-27308554 | MS.gene071445:intron | 35.0% |
| !!! | AAATGCTTTTGAAATTGGCC+AGG | - | chr1.2:27307978-27307997 | MS.gene071445:CDS | 35.0% |
| !!! | AAGGGCCATTTTAATTCCTT+TGG | + | chr1.2:27308221-27308240 | None:intergenic | 35.0% |
| AAACAGGTGTCTCTACTCTT+TGG | - | chr1.2:27308570-27308589 | MS.gene071445:CDS | 40.0% | |
| AACAGGTGTCTCTACTCTTT+GGG | - | chr1.2:27308571-27308590 | MS.gene071445:CDS | 40.0% | |
| AAGAGCTAAGGTATGTCCAA+AGG | - | chr1.2:27308202-27308221 | MS.gene071445:intron | 40.0% | |
| CATAGATATGGCTGCATCTT+TGG | - | chr1.2:27308085-27308104 | MS.gene071445:intron | 40.0% | |
| CTCATATTCCTCTCCTACTT+GGG | - | chr1.2:27308026-27308045 | MS.gene071445:intron | 40.0% | |
| TCATATTCCTCTCCTACTTG+GGG | - | chr1.2:27308027-27308046 | MS.gene071445:intron | 40.0% | |
| TCTCATATTCCTCTCCTACT+TGG | - | chr1.2:27308025-27308044 | MS.gene071445:intron | 40.0% | |
| TGATTGTGAGGTTCATGCTT+TGG | - | chr1.2:27308472-27308491 | MS.gene071445:intron | 40.0% | |
| TGTGACATACAGTGAAGACA+AGG | + | chr1.2:27308293-27308312 | None:intergenic | 40.0% | |
| !! | TTTTGCATTTCTGTGCTGGT+TGG | - | chr1.2:27308316-27308335 | MS.gene071445:intron | 40.0% |
| AGAGTAGAGACACCTGTTTC+TGG | + | chr1.2:27308569-27308588 | None:intergenic | 45.0% | |
| GATATGGCTGCATCTTTGGA+AGG | - | chr1.2:27308089-27308108 | MS.gene071445:intron | 45.0% | |
| ! | AGTGGGATTTTGAACAGCCA+TGG | + | chr1.2:27308362-27308381 | None:intergenic | 45.0% |
| !! | GTGTCTCTACTCTTTGGGAT+TGG | - | chr1.2:27308576-27308595 | MS.gene071445:CDS | 45.0% |
| !! | TGAGGTTCATGCTTTGGAAC+AGG | - | chr1.2:27308478-27308497 | MS.gene071445:intron | 45.0% |
| CGGGAACTCATTCCAGAAAC+AGG | - | chr1.2:27308554-27308573 | MS.gene071445:intron | 50.0% | |
| TCAAGTACCCCAAGTAGGAG+AGG | + | chr1.2:27308037-27308056 | None:intergenic | 50.0% | |
| !! | CTTTGGGATTGGCCATGCTG+TGG | - | chr1.2:27308587-27308606 | MS.gene071445:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 27307955 | 27308611 | 27307955 | ID=MS.gene071445 |
| chr1.2 | mRNA | 27307955 | 27308611 | 27307955 | ID=MS.gene071445.t1;Parent=MS.gene071445 |
| chr1.2 | exon | 27308567 | 27308611 | 27308567 | ID=MS.gene071445.t1.exon1;Parent=MS.gene071445.t1 |
| chr1.2 | CDS | 27308567 | 27308611 | 27308567 | ID=cds.MS.gene071445.t1;Parent=MS.gene071445.t1 |
| chr1.2 | exon | 27308355 | 27308486 | 27308355 | ID=MS.gene071445.t1.exon2;Parent=MS.gene071445.t1 |
| chr1.2 | CDS | 27308355 | 27308486 | 27308355 | ID=cds.MS.gene071445.t1;Parent=MS.gene071445.t1 |
| chr1.2 | exon | 27307955 | 27308017 | 27307955 | ID=MS.gene071445.t1.exon3;Parent=MS.gene071445.t1 |
| chr1.2 | CDS | 27307955 | 27308017 | 27307955 | ID=cds.MS.gene071445.t1;Parent=MS.gene071445.t1 |
| Gene Sequence |
| Protein sequence |
