AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072101

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072101.t1 MTR_2g005350 97.712 437 5 4 1 434 4 438 0.0 864
MS.gene072101.t1 MTR_1g016880 56.918 318 134 3 119 434 60 376 7.60e-131 382
MS.gene072101.t1 MTR_1g110290 52.812 320 148 2 116 434 76 393 1.31e-121 360
MS.gene072101.t1 MTR_5g055480 52.258 310 146 1 125 434 83 390 1.02e-120 357
MS.gene072101.t1 MTR_5g006930 45.897 390 196 3 46 434 11 386 3.78e-120 355
MS.gene072101.t1 MTR_8g015500 53.770 305 139 2 130 433 109 412 1.22e-117 350
MS.gene072101.t1 MTR_5g006920 43.645 417 198 5 31 434 29 421 1.19e-116 348
MS.gene072101.t1 MTR_6g083830 52.427 309 145 2 127 434 112 419 1.70e-116 347
MS.gene072101.t1 MTR_3g104840 47.756 312 143 4 124 434 101 393 1.33e-100 305
MS.gene072101.t1 MTR_3g108740 41.824 318 162 5 131 427 58 373 5.86e-75 239
MS.gene072101.t1 MTR_1g009960 43.000 300 149 3 155 434 102 399 6.44e-73 234
MS.gene072101.t1 MTR_4g127550 41.667 288 147 3 161 429 104 389 6.34e-70 226
MS.gene072101.t1 MTR_1g009960 46.721 244 122 2 155 392 102 343 2.86e-68 221
MS.gene072101.t1 MTR_2g037600 43.007 286 141 4 164 429 105 388 3.40e-68 222
MS.gene072101.t1 MTR_2g037620 41.754 285 145 3 164 429 97 379 2.58e-67 219
MS.gene072101.t1 MTR_2g024120 39.145 304 138 10 164 425 61 359 4.07e-51 177
MS.gene072101.t1 MTR_8g094180 36.624 314 144 11 164 428 55 362 2.03e-48 169
MS.gene072101.t1 MTR_3g106930 37.179 312 180 8 133 432 67 374 1.57e-46 164
MS.gene072101.t1 MTR_8g094180 37.879 264 127 8 164 394 55 314 5.06e-45 159
MS.gene072101.t1 MTR_1g048320 38.062 289 148 10 164 432 64 341 6.12e-41 149
MS.gene072101.t1 MTR_2g087610 34.768 302 149 10 165 432 78 365 1.16e-39 146
MS.gene072101.t1 MTR_1g031060 34.364 291 158 8 164 432 80 359 1.78e-33 129
MS.gene072101.t1 MTR_1g031080 35.223 247 130 7 207 432 52 289 1.82e-31 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072101.t1 AT1G10280 57.143 434 161 6 2 434 3 412 0.0 511
MS.gene072101.t1 AT5G11730 55.960 302 132 1 133 434 86 386 8.75e-126 370
MS.gene072101.t1 AT5G25970 54.430 316 142 2 119 433 72 386 1.00e-124 367
MS.gene072101.t1 AT5G25970 54.430 316 142 2 119 433 72 386 1.00e-124 367
MS.gene072101.t1 AT5G25970 54.430 316 142 2 119 433 72 386 1.00e-124 367
MS.gene072101.t1 AT1G51770 54.952 313 139 2 123 434 81 392 2.62e-124 367
MS.gene072101.t1 AT3G21310 54.777 314 140 2 122 434 71 383 1.14e-122 362
MS.gene072101.t1 AT3G21310 54.777 314 140 2 122 434 71 383 1.14e-122 362
MS.gene072101.t1 AT1G68390 55.238 315 135 4 124 434 96 408 2.49e-119 354
MS.gene072101.t1 AT1G68380 52.294 327 146 6 113 434 71 392 1.03e-109 329
MS.gene072101.t1 AT1G73810 51.792 307 143 2 130 434 115 418 1.03e-109 330
MS.gene072101.t1 AT1G51770 50.160 313 127 3 123 434 81 365 1.01e-107 323
MS.gene072101.t1 AT1G10880 51.736 288 137 2 141 428 95 380 6.10e-107 322
MS.gene072101.t1 AT5G16170 48.265 317 145 3 136 434 94 409 6.46e-103 312
MS.gene072101.t1 AT4G31350 43.110 283 140 3 164 427 88 368 5.08e-74 236
MS.gene072101.t1 AT4G31350 43.110 283 140 3 164 427 88 368 5.08e-74 236
MS.gene072101.t1 AT4G31350 43.110 283 140 3 164 427 88 368 5.08e-74 236
MS.gene072101.t1 AT4G30060 44.755 286 134 4 165 429 109 391 5.06e-71 229
MS.gene072101.t1 AT4G30060 44.755 286 134 4 165 429 109 391 5.06e-71 229
MS.gene072101.t1 AT4G25870 40.979 327 165 8 124 427 59 380 5.75e-71 229
MS.gene072101.t1 AT4G31350 42.403 283 137 4 164 427 88 363 9.30e-69 223
MS.gene072101.t1 AT2G19160 42.403 283 141 3 165 427 106 386 5.20e-68 221
MS.gene072101.t1 AT2G19160 42.403 283 141 3 165 427 106 386 5.20e-68 221
MS.gene072101.t1 AT5G57270 42.958 284 139 4 165 427 98 379 1.84e-67 220
MS.gene072101.t1 AT5G57270 42.958 284 139 4 165 427 98 379 1.84e-67 220
MS.gene072101.t1 AT5G57270 42.958 284 139 4 165 427 98 379 1.84e-67 220
MS.gene072101.t1 AT5G57270 42.958 284 139 4 165 427 98 379 1.84e-67 220
MS.gene072101.t1 AT5G57270 42.958 284 139 4 165 427 98 379 1.84e-67 220
MS.gene072101.t1 AT3G21310 56.111 180 78 1 122 300 71 250 6.59e-64 207
MS.gene072101.t1 AT1G62305 39.604 303 136 10 164 425 68 364 1.08e-54 186
MS.gene072101.t1 AT3G52060 38.571 280 151 8 164 432 74 343 9.50e-53 180
MS.gene072101.t1 AT1G11940 39.597 298 134 10 164 420 73 365 5.77e-52 179
MS.gene072101.t1 AT5G25330 40.860 279 154 7 165 432 81 359 1.03e-50 176
MS.gene072101.t1 AT5G14550 39.357 249 129 5 164 393 64 309 1.87e-49 172
MS.gene072101.t1 AT5G14550 36.275 306 150 9 164 427 64 366 6.09e-49 171
MS.gene072101.t1 AT5G14550 36.393 305 149 9 164 426 64 365 7.89e-49 171
MS.gene072101.t1 AT1G62305 43.396 212 94 5 164 353 68 275 8.22e-47 164
MS.gene072101.t1 AT1G11940 47.753 178 86 4 164 337 73 247 2.17e-43 153
MS.gene072101.t1 AT1G62305 35.314 303 125 10 164 425 68 340 2.86e-39 145
MS.gene072101.t1 AT5G22070 34.437 302 151 11 165 432 71 359 3.95e-39 144
MS.gene072101.t1 AT4G32290 38.889 288 152 9 165 432 93 376 5.81e-39 144
MS.gene072101.t1 AT5G14550 34.812 293 145 10 164 427 64 339 7.48e-39 143

Find 128 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTGTTCTGTTTGAATTA+AGG 0.235975 2.4:-76621347 None:intergenic
GGTGGTGGAGGAGGAGGAGG+AGG 0.258518 2.4:-76620390 None:intergenic
CTTATTCACAGGCTGTTTCT+TGG 0.263757 2.4:+76620929 MS.gene072101:intron
TGGGATATTGCGTCGATAAA+AGG 0.273998 2.4:-76620691 None:intergenic
TTATTCACAGGCTGTTTCTT+GGG 0.283167 2.4:+76620930 MS.gene072101:intron
TAGGGATAGTGGTATTTCTT+AGG 0.304256 2.4:-76620513 None:intergenic
GGGAACCATGGAACATATTC+AGG 0.311181 2.4:-76621326 None:intergenic
GAAGTCATATCTGAATTATA+TGG 0.351240 2.4:-76621474 None:intergenic
ACGGAAGAGGGAAAAGTATT+GGG 0.351893 2.4:-76621253 None:intergenic
AAGCAGGCTTACAGTATTTA+CGG 0.358219 2.4:-76621272 None:intergenic
GACTGTTCTGTTTGAATTAA+GGG 0.359001 2.4:-76621346 None:intergenic
CTTTACCCTTGTTGCCACTC+TGG 0.363568 2.4:+76620580 MS.gene072101:CDS
GTTGGAAGGTAGTGTTCATC+TGG 0.377430 2.4:-76621300 None:intergenic
TTGTCCCTCCCGTAGGTTGC+CGG 0.393192 2.4:-76621402 None:intergenic
TGTTGTTGTTGGGTTGTTGT+TGG 0.395852 2.4:-76620314 None:intergenic
TATGATGATCCTTCCCGTTA+TGG 0.399415 2.4:+76621117 MS.gene072101:CDS
TTTGCTGCTGGTGTCGTCAT+TGG 0.399698 2.4:+76620162 MS.gene072101:CDS
TACGGAAGAGGGAAAAGTAT+TGG 0.403770 2.4:-76621254 None:intergenic
CTCCCGTAGGTTGCCGGATG+AGG 0.404141 2.4:-76621396 None:intergenic
GCACTCTGGTTTGGTTAGAT+TGG 0.409919 2.4:+76620110 MS.gene072101:CDS
CAGAAGAAGAAGAAGGTTGT+TGG 0.421210 2.4:-76620257 None:intergenic
ATGCGTGGAGGAGGAGAAGA+AGG 0.422782 2.4:+76620057 MS.gene072101:CDS
TGCTGTTGCTGTTGCTTCGT+CGG 0.423883 2.4:-76620090 None:intergenic
AGGTACATGAAAGCGAGTTT+AGG 0.428382 2.4:-76620546 None:intergenic
CAGTGCTTAAGCTGAATATC+AGG 0.430715 2.4:-76621168 None:intergenic
TATTGCGGCTGTAGCGGCCT+CGG 0.431543 2.4:-76621140 None:intergenic
GTAGCGGCCTCGGCCATAAC+GGG 0.437948 2.4:-76621130 None:intergenic
GGTCCTCATCCGGCAACCTA+CGG 0.443893 2.4:+76621393 MS.gene072101:CDS
AGAGAAGGTGTGGGTGATGT+TGG 0.444842 2.4:-76620457 None:intergenic
TTTACCCTTGTTGCCACTCT+GGG 0.445219 2.4:+76620581 MS.gene072101:CDS
ACAGCAACAGCACTCTGGTT+TGG 0.447376 2.4:+76620101 MS.gene072101:CDS
GGATATAAATGTTGAAGAGA+TGG 0.459423 2.4:-76620626 None:intergenic
AAGCAAGGGCTCCAATGCAC+TGG 0.459653 2.4:-76621529 None:intergenic
GGGATATTGCGTCGATAAAA+GGG 0.460802 2.4:-76620690 None:intergenic
TGTAGCGGCCTCGGCCATAA+CGG 0.463805 2.4:-76621131 None:intergenic
CATATCCTCCTCTTCGGAGG+GGG 0.463862 2.4:-76620412 None:intergenic
AGAAGAAGAAGAAGGTTGTT+GGG 0.466492 2.4:-76620256 None:intergenic
TGTTGTTGGGTTGTTGTTGG+AGG 0.466863 2.4:-76620311 None:intergenic
CTCTGGGAGAGATTCTTTCA+AGG 0.470451 2.4:+76620597 MS.gene072101:CDS
ATCTGAATTATATGGACAAA+GGG 0.472207 2.4:-76621466 None:intergenic
CAAGTTTGATCCCAGTGCAT+TGG 0.474721 2.4:+76621518 MS.gene072101:CDS
GTTGTTGGGTTGTTGTTGGA+GGG 0.477667 2.4:-76620310 None:intergenic
GTCAATGCTCGGTCCTCATC+CGG 0.486872 2.4:+76621383 MS.gene072101:CDS
ATGGCGAGGATGCGTGGAGG+AGG 0.491011 2.4:+76620048 None:intergenic
GTCCTCATCCGGCAACCTAC+GGG 0.492307 2.4:+76621394 MS.gene072101:CDS
ATGAGATATCTGTAAACAGT+AGG 0.496818 2.4:-76621066 None:intergenic
GTACACGAGCGTAGGGATAG+TGG 0.503892 2.4:-76620524 None:intergenic
ATTTATATCCACGCTCCTCC+TGG 0.504909 2.4:+76620639 MS.gene072101:CDS
TATTCACAGGCTGTTTCTTG+GGG 0.505590 2.4:+76620931 MS.gene072101:intron
TCGACGCAATATCCCAAGTC+AGG 0.507097 2.4:+76620698 MS.gene072101:CDS
AGGAAGCCCTCCAGAAAAGT+TGG 0.507783 2.4:-76620485 None:intergenic
ACAGTAGGGAAGTTGTAGAC+TGG 0.509799 2.4:-76621051 None:intergenic
CAGTAGGGAAGTTGTAGACT+GGG 0.512385 2.4:-76621050 None:intergenic
AACATATCCTCCTCTTCGGA+GGG 0.513482 2.4:-76620414 None:intergenic
CTCCAACATATCCTCCTCTT+CGG 0.513489 2.4:-76620418 None:intergenic
TGTTCATCTGGGTAGCAAGC+AGG 0.515330 2.4:-76621288 None:intergenic
CAGAGTGGCAACAAGGGTAA+AGG 0.517032 2.4:-76620579 None:intergenic
GATCCTTCCCGTTATGGCCG+AGG 0.520104 2.4:+76621123 MS.gene072101:CDS
CTCCGAAGAGGAGGATATGT+TGG 0.522889 2.4:+76620416 MS.gene072101:CDS
CTTACAGTATTTACGGAAGA+GGG 0.523130 2.4:-76621265 None:intergenic
TCGCTTTCATGTACCTCACC+AGG 0.525613 2.4:+76620553 MS.gene072101:CDS
TGAAAAGTCGAGCAGTGCAT+TGG 0.525633 2.4:-76620992 None:intergenic
GATATAAATGTTGAAGAGAT+GGG 0.527961 2.4:-76620625 None:intergenic
GCGAGCAAAGAGGTAACAAA+TGG 0.530365 2.4:-76621496 None:intergenic
CTGATATTCAGCTTAAGCAC+TGG 0.530590 2.4:+76621169 MS.gene072101:CDS
CAGCTTAAGCACTGGCGCAA+AGG 0.531196 2.4:+76621177 MS.gene072101:CDS
GCTTACAGTATTTACGGAAG+AGG 0.538011 2.4:-76621266 None:intergenic
TGGAACATATTCAGGAAGGT+TGG 0.538336 2.4:-76621318 None:intergenic
GAACAGTCACATGGGTCGAC+TGG 0.550051 2.4:+76621361 MS.gene072101:CDS
GATGTTGGAAGGATGAAGAA+AGG 0.550089 2.4:-76620442 None:intergenic
AAATTAAAAGTTCATGACTT+CGG 0.551140 2.4:-76621562 None:intergenic
CAACAAGGGTAAAGGTCCCC+TGG 0.554561 2.4:-76620571 None:intergenic
GAAGCATATTGCGGCTGTAG+CGG 0.557435 2.4:-76621146 None:intergenic
GATTGGATGGCGAGGATGCG+TGG 0.560283 2.4:+76620042 None:intergenic
TTCTTGGGGAACAGTTACTC+TGG 0.561336 2.4:+76620945 MS.gene072101:CDS
ACCTTCCTGAATATGTTCCA+TGG 0.561364 2.4:+76621321 MS.gene072101:CDS
ACTGGCGCAAAGGCTCTCAA+TGG 0.562161 2.4:+76621187 MS.gene072101:CDS
AGCAAGGGCTCCAATGCACT+GGG 0.565315 2.4:-76621528 None:intergenic
AAGACATCAGAAGAAGAAGA+AGG 0.565316 2.4:-76620264 None:intergenic
AGTTATATTGTCCCTCCCGT+AGG 0.573521 2.4:-76621409 None:intergenic
CAACATATCCTCCTCTTCGG+AGG 0.575606 2.4:-76620415 None:intergenic
TTCGGAAGAGAGGTCAAGCA+AGG 0.577351 2.4:-76621544 None:intergenic
TATCTGAATTATATGGACAA+AGG 0.578382 2.4:-76621467 None:intergenic
TGGGTCGACTGGTCAATGCT+CGG 0.578827 2.4:+76621372 MS.gene072101:CDS
ATATCCTCCTCTTCGGAGGG+GGG 0.578887 2.4:-76620411 None:intergenic
GAAAAGTTGGTGGTCAGAGA+AGG 0.580866 2.4:-76620472 None:intergenic
CTCTGGCCGATGCGGAGAGG+CGG 0.581736 2.4:+76620962 MS.gene072101:CDS
CTCTCCCAGAGTGGCAACAA+GGG 0.584168 2.4:-76620585 None:intergenic
AGTTTAGGTACACGAGCGTA+GGG 0.587019 2.4:-76620531 None:intergenic
TAGTAGCCGCCTCTCCGCAT+CGG 0.587573 2.4:-76620968 None:intergenic
TGTTTGAATTAAGGGAACCA+TGG 0.588224 2.4:-76621338 None:intergenic
TGAGATATCTGTAAACAGTA+GGG 0.589706 2.4:-76621065 None:intergenic
ACATATTCAGGAAGGTTGGA+AGG 0.590150 2.4:-76621314 None:intergenic
TCTCTCCCAGAGTGGCAACA+AGG 0.591099 2.4:-76620586 None:intergenic
GTTGGTGGTCAGAGAAGGTG+TGG 0.594958 2.4:-76620467 None:intergenic
ATACAGGCTATAAGGAACAA+TGG 0.597495 2.4:+76621441 MS.gene072101:CDS
CTCATCCGGCAACCTACGGG+AGG 0.602306 2.4:+76621397 MS.gene072101:CDS
AAGGTGTGGGTGATGTTGGA+AGG 0.605998 2.4:-76620453 None:intergenic
GAGTTTAGGTACACGAGCGT+AGG 0.606394 2.4:-76620532 None:intergenic
AACAGTTACTCTGGCCGATG+CGG 0.608462 2.4:+76620954 MS.gene072101:CDS
AGTTCATGACTTCGGAAGAG+AGG 0.611942 2.4:-76621554 None:intergenic
ACATATCCTCCTCTTCGGAG+GGG 0.614099 2.4:-76620413 None:intergenic
GTGGTATTTCTTAGGAACCA+AGG 0.614170 2.4:-76620505 None:intergenic
AAATGTTGAAGAGATGGGAG+TGG 0.614619 2.4:-76620620 None:intergenic
AGGGTAAAGGTCCCCTGGTG+AGG 0.616195 2.4:-76620566 None:intergenic
AAGCCCTCCAGAAAAGTTGG+TGG 0.617230 2.4:-76620482 None:intergenic
AAGCAACAGCAACAGCACTC+TGG 0.619654 2.4:+76620096 MS.gene072101:CDS
CGGCCTCGGCCATAACGGGA+AGG 0.626778 2.4:-76621126 None:intergenic
TTGGAAGGTAGTGTTCATCT+GGG 0.631422 2.4:-76621299 None:intergenic
CGCTTTCATGTACCTCACCA+GGG 0.635577 2.4:+76620554 MS.gene072101:CDS
GAATATAACCAGGAGGAGCG+TGG 0.637995 2.4:-76620647 None:intergenic
CTATATAAACAGCAAGTGCA+CGG 0.643830 2.4:-76621221 None:intergenic
ATTCAAACAGAACAGTCACA+TGG 0.646779 2.4:+76621352 MS.gene072101:CDS
GAATATCAGGAAGCATATTG+CGG 0.649024 2.4:-76621155 None:intergenic
GGGAGGGACAATATAACTGT+CGG 0.656711 2.4:+76621414 MS.gene072101:CDS
GATCAAACTTGCGAGCAAAG+AGG 0.662138 2.4:-76621506 None:intergenic
TGGATGGCGAGGATGCGTGG+AGG 0.662304 2.4:+76620045 None:intergenic
ACCATGGAACATATTCAGGA+AGG 0.662805 2.4:-76621322 None:intergenic
TCGGAAGAGAGGTCAAGCAA+GGG 0.668978 2.4:-76621543 None:intergenic
TTCAAACAGAACAGTCACAT+GGG 0.669144 2.4:+76621353 MS.gene072101:CDS
TCATCCGGCAACCTACGGGA+GGG 0.670560 2.4:+76621398 MS.gene072101:CDS
TTACTCTGGCCGATGCGGAG+AGG 0.672407 2.4:+76620959 MS.gene072101:CDS
GTAGTGAATGTGAGAAGTTG+TGG 0.678670 2.4:-76620190 None:intergenic
TGAAAGAATCTCTCCCAGAG+TGG 0.689461 2.4:-76620594 None:intergenic
GCTTTCATGTACCTCACCAG+GGG 0.715038 2.4:+76620555 MS.gene072101:CDS
GAAACGTTGAGAATATAACC+AGG 0.715720 2.4:-76620657 None:intergenic
TTGGTGGTCAGAGAAGGTGT+GGG 0.735159 2.4:-76620466 None:intergenic
ACGTTGAGAATATAACCAGG+AGG 0.765757 2.4:-76620654 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGTTTTGATGATTTAGT+GGG - chr2.4:76620859-76620878 None:intergenic 20.0%
!!! TTATAGTTTTGATGATTTAG+TGG - chr2.4:76620860-76620879 None:intergenic 20.0%
! AAGTTTCCTTGATAGAATTA+AGG - chr2.4:76620734-76620753 None:intergenic 25.0%
! AATGAATTGTTTGTACTTGT+AGG + chr2.4:76620769-76620788 MS.gene072101:intron 25.0%
! AGTTTCCTTGATAGAATTAA+GGG - chr2.4:76620733-76620752 None:intergenic 25.0%
! ATCTGAATTATATGGACAAA+GGG - chr2.4:76621469-76621488 None:intergenic 25.0%
! ATGAATTGTTTGTACTTGTA+GGG + chr2.4:76620770-76620789 MS.gene072101:intron 25.0%
! GAAGTCATATCTGAATTATA+TGG - chr2.4:76621477-76621496 None:intergenic 25.0%
! GATATAAATGTTGAAGAGAT+GGG - chr2.4:76620628-76620647 None:intergenic 25.0%
! GTTTATGATTCATTCATACA+TGG - chr2.4:76620808-76620827 None:intergenic 25.0%
! TATCTGAATTATATGGACAA+AGG - chr2.4:76621470-76621489 None:intergenic 25.0%
! TGAAAATATGAGTAAAGAGA+TGG - chr2.4:76620834-76620853 None:intergenic 25.0%
AATATGAGTAAAGAGATGGA+TGG - chr2.4:76620830-76620849 None:intergenic 30.0%
ATGAGATATCTGTAAACAGT+AGG - chr2.4:76621069-76621088 None:intergenic 30.0%
GACTGTTCTGTTTGAATTAA+GGG - chr2.4:76621349-76621368 None:intergenic 30.0%
GGATATAAATGTTGAAGAGA+TGG - chr2.4:76620629-76620648 None:intergenic 30.0%
GTTTCCTTGATAGAATTAAG+GGG - chr2.4:76620732-76620751 None:intergenic 30.0%
TGACTGTTCTGTTTGAATTA+AGG - chr2.4:76621350-76621369 None:intergenic 30.0%
TGAGATATCTGTAAACAGTA+GGG - chr2.4:76621068-76621087 None:intergenic 30.0%
TTTCCTTGATAGAATTAAGG+GGG - chr2.4:76620731-76620750 None:intergenic 30.0%
! TTGTTGTTGTTGTTGTTGTT+GGG - chr2.4:76620327-76620346 None:intergenic 30.0%
!! ATGAGTTTTGTCTTATTCAC+AGG + chr2.4:76620918-76620937 MS.gene072101:intron 30.0%
!!! TATAACTGTCGGTTTTATAC+AGG + chr2.4:76621425-76621444 MS.gene072101:CDS 30.0%
AAGACATCAGAAGAAGAAGA+AGG - chr2.4:76620267-76620286 None:intergenic 35.0%
AAGCAGGCTTACAGTATTTA+CGG - chr2.4:76621275-76621294 None:intergenic 35.0%
AGAAGAAGAAGAAGGTTGTT+GGG - chr2.4:76620259-76620278 None:intergenic 35.0%
ATACAGGCTATAAGGAACAA+TGG + chr2.4:76621441-76621460 MS.gene072101:CDS 35.0%
ATTCAAACAGAACAGTCACA+TGG + chr2.4:76621352-76621371 MS.gene072101:CDS 35.0%
CTATATAAACAGCAAGTGCA+CGG - chr2.4:76621224-76621243 None:intergenic 35.0%
CTTACAGTATTTACGGAAGA+GGG - chr2.4:76621268-76621287 None:intergenic 35.0%
GAAACGTTGAGAATATAACC+AGG - chr2.4:76620660-76620679 None:intergenic 35.0%
TGTTTGAATTAAGGGAACCA+TGG - chr2.4:76621341-76621360 None:intergenic 35.0%
TTCAAACAGAACAGTCACAT+GGG + chr2.4:76621353-76621372 MS.gene072101:CDS 35.0%
! GAATATCAGGAAGCATATTG+CGG - chr2.4:76621158-76621177 None:intergenic 35.0%
! GTTGTTGTTGTTGTTGTTGT+TGG - chr2.4:76620328-76620347 None:intergenic 35.0%
! TAGGGATAGTGGTATTTCTT+AGG - chr2.4:76620516-76620535 None:intergenic 35.0%
!! TCGGTTTTATACAGGCTATA+AGG + chr2.4:76621433-76621452 MS.gene072101:CDS 35.0%
!! TTATTCACAGGCTGTTTCTT+GGG + chr2.4:76620930-76620949 MS.gene072101:intron 35.0%
AAATGTTGAAGAGATGGGAG+TGG - chr2.4:76620623-76620642 None:intergenic 40.0%
ACATATTCAGGAAGGTTGGA+AGG - chr2.4:76621317-76621336 None:intergenic 40.0%
ACCATGGAACATATTCAGGA+AGG - chr2.4:76621325-76621344 None:intergenic 40.0%
ACCTTCCTGAATATGTTCCA+TGG + chr2.4:76621321-76621340 MS.gene072101:CDS 40.0%
ACGTTGAGAATATAACCAGG+AGG - chr2.4:76620657-76620676 None:intergenic 40.0%
AGGTACATGAAAGCGAGTTT+AGG - chr2.4:76620549-76620568 None:intergenic 40.0%
CAGAAGAAGAAGAAGGTTGT+TGG - chr2.4:76620260-76620279 None:intergenic 40.0%
CAGTGCTTAAGCTGAATATC+AGG - chr2.4:76621171-76621190 None:intergenic 40.0%
CATCCCCCTTAATTCTATCA+AGG + chr2.4:76620725-76620744 MS.gene072101:intron 40.0%
CTGATATTCAGCTTAAGCAC+TGG + chr2.4:76621169-76621188 MS.gene072101:CDS 40.0%
GATGTTGGAAGGATGAAGAA+AGG - chr2.4:76620445-76620464 None:intergenic 40.0%
GCTTACAGTATTTACGGAAG+AGG - chr2.4:76621269-76621288 None:intergenic 40.0%
GTAGTGAATGTGAGAAGTTG+TGG - chr2.4:76620193-76620212 None:intergenic 40.0%
GTGGTATTTCTTAGGAACCA+AGG - chr2.4:76620508-76620527 None:intergenic 40.0%
TATGATGATCCTTCCCGTTA+TGG + chr2.4:76621117-76621136 MS.gene072101:CDS 40.0%
TGGAACATATTCAGGAAGGT+TGG - chr2.4:76621321-76621340 None:intergenic 40.0%
TTGGAAGGTAGTGTTCATCT+GGG - chr2.4:76621302-76621321 None:intergenic 40.0%
! GGGATATTGCGTCGATAAAA+GGG - chr2.4:76620693-76620712 None:intergenic 40.0%
! TACGGAAGAGGGAAAAGTAT+TGG - chr2.4:76621257-76621276 None:intergenic 40.0%
! TGGGATATTGCGTCGATAAA+AGG - chr2.4:76620694-76620713 None:intergenic 40.0%
! TGTTGTTGTTGGGTTGTTGT+TGG - chr2.4:76620317-76620336 None:intergenic 40.0%
!! ACGGAAGAGGGAAAAGTATT+GGG - chr2.4:76621256-76621275 None:intergenic 40.0%
!! CTTATTCACAGGCTGTTTCT+TGG + chr2.4:76620929-76620948 MS.gene072101:intron 40.0%
!! TATTCACAGGCTGTTTCTTG+GGG + chr2.4:76620931-76620950 MS.gene072101:intron 40.0%
!!! TTGTTGCTTGTTTTTGCTGC+TGG + chr2.4:76620150-76620169 MS.gene072101:CDS 40.0%
AACATATCCTCCTCTTCGGA+GGG - chr2.4:76620417-76620436 None:intergenic 45.0%
ACAGTAGGGAAGTTGTAGAC+TGG - chr2.4:76621054-76621073 None:intergenic 45.0%
AGTTATATTGTCCCTCCCGT+AGG - chr2.4:76621412-76621431 None:intergenic 45.0%
AGTTCATGACTTCGGAAGAG+AGG - chr2.4:76621557-76621576 None:intergenic 45.0%
AGTTTAGGTACACGAGCGTA+GGG - chr2.4:76620534-76620553 None:intergenic 45.0%
ATTTATATCCACGCTCCTCC+TGG + chr2.4:76620639-76620658 MS.gene072101:CDS 45.0%
CAAGTTTGATCCCAGTGCAT+TGG + chr2.4:76621518-76621537 MS.gene072101:CDS 45.0%
CAGTAGGGAAGTTGTAGACT+GGG - chr2.4:76621053-76621072 None:intergenic 45.0%
CTCCAACATATCCTCCTCTT+CGG - chr2.4:76620421-76620440 None:intergenic 45.0%
GATCAAACTTGCGAGCAAAG+AGG - chr2.4:76621509-76621528 None:intergenic 45.0%
GCGAGCAAAGAGGTAACAAA+TGG - chr2.4:76621499-76621518 None:intergenic 45.0%
GGGAACCATGGAACATATTC+AGG - chr2.4:76621329-76621348 None:intergenic 45.0%
GGGAGGGACAATATAACTGT+CGG + chr2.4:76621414-76621433 MS.gene072101:CDS 45.0%
GTTGGAAGGTAGTGTTCATC+TGG - chr2.4:76621303-76621322 None:intergenic 45.0%
TGAAAAGTCGAGCAGTGCAT+TGG - chr2.4:76620995-76621014 None:intergenic 45.0%
TGAAAGAATCTCTCCCAGAG+TGG - chr2.4:76620597-76620616 None:intergenic 45.0%
TTCTTGGGGAACAGTTACTC+TGG + chr2.4:76620945-76620964 MS.gene072101:CDS 45.0%
TTTACCCTTGTTGCCACTCT+GGG + chr2.4:76620581-76620600 MS.gene072101:CDS 45.0%
! CTCTGGGAGAGATTCTTTCA+AGG + chr2.4:76620597-76620616 MS.gene072101:CDS 45.0%
! GTTGTTGGGTTGTTGTTGGA+GGG - chr2.4:76620313-76620332 None:intergenic 45.0%
! TCTCTGACCACCAACTTTTC+TGG + chr2.4:76620475-76620494 MS.gene072101:CDS 45.0%
! TGACCACCAACTTTTCTGGA+GGG + chr2.4:76620479-76620498 MS.gene072101:CDS 45.0%
! TGTTGTTGGGTTGTTGTTGG+AGG - chr2.4:76620314-76620333 None:intergenic 45.0%
!! GAAAAGTTGGTGGTCAGAGA+AGG - chr2.4:76620475-76620494 None:intergenic 45.0%
!! GCACTCTGGTTTGGTTAGAT+TGG + chr2.4:76620110-76620129 MS.gene072101:CDS 45.0%
AACAGTTACTCTGGCCGATG+CGG + chr2.4:76620954-76620973 MS.gene072101:CDS 50.0%
AAGCAACAGCAACAGCACTC+TGG + chr2.4:76620096-76620115 MS.gene072101:CDS 50.0%
AAGCCCTCCAGAAAAGTTGG+TGG - chr2.4:76620485-76620504 None:intergenic 50.0%
AAGGTGTGGGTGATGTTGGA+AGG - chr2.4:76620456-76620475 None:intergenic 50.0%
ACAGCAACAGCACTCTGGTT+TGG + chr2.4:76620101-76620120 MS.gene072101:CDS 50.0%
ACATATCCTCCTCTTCGGAG+GGG - chr2.4:76620416-76620435 None:intergenic 50.0%
AGGAAGCCCTCCAGAAAAGT+TGG - chr2.4:76620488-76620507 None:intergenic 50.0%
CAACATATCCTCCTCTTCGG+AGG - chr2.4:76620418-76620437 None:intergenic 50.0%
CAGAGTGGCAACAAGGGTAA+AGG - chr2.4:76620582-76620601 None:intergenic 50.0%
CGCTTTCATGTACCTCACCA+GGG + chr2.4:76620554-76620573 MS.gene072101:CDS 50.0%
CTCCGAAGAGGAGGATATGT+TGG + chr2.4:76620416-76620435 MS.gene072101:CDS 50.0%
CTTTACCCTTGTTGCCACTC+TGG + chr2.4:76620580-76620599 MS.gene072101:CDS 50.0%
GAAGCATATTGCGGCTGTAG+CGG - chr2.4:76621149-76621168 None:intergenic 50.0%
GAATATAACCAGGAGGAGCG+TGG - chr2.4:76620650-76620669 None:intergenic 50.0%
GAGTTTAGGTACACGAGCGT+AGG - chr2.4:76620535-76620554 None:intergenic 50.0%
GCTTTCATGTACCTCACCAG+GGG + chr2.4:76620555-76620574 MS.gene072101:CDS 50.0%
TCGACGCAATATCCCAAGTC+AGG + chr2.4:76620698-76620717 MS.gene072101:CDS 50.0%
TCGCTTTCATGTACCTCACC+AGG + chr2.4:76620553-76620572 MS.gene072101:CDS 50.0%
TCGGAAGAGAGGTCAAGCAA+GGG - chr2.4:76621546-76621565 None:intergenic 50.0%
TGTTCATCTGGGTAGCAAGC+AGG - chr2.4:76621291-76621310 None:intergenic 50.0%
TTCGGAAGAGAGGTCAAGCA+AGG - chr2.4:76621547-76621566 None:intergenic 50.0%
! AGAGAAGGTGTGGGTGATGT+TGG - chr2.4:76620460-76620479 None:intergenic 50.0%
! CTGACCACCAACTTTTCTGG+AGG + chr2.4:76620478-76620497 MS.gene072101:CDS 50.0%
! TGCTGTTGCTGTTGCTTCGT+CGG - chr2.4:76620093-76620112 None:intergenic 50.0%
! TTGGTGGTCAGAGAAGGTGT+GGG - chr2.4:76620469-76620488 None:intergenic 50.0%
!! ACTTTTCTGGAGGGCTTCCT+TGG + chr2.4:76620488-76620507 MS.gene072101:CDS 50.0%
!! TTTGCTGCTGGTGTCGTCAT+TGG + chr2.4:76620162-76620181 MS.gene072101:CDS 50.0%
AAGCAAGGGCTCCAATGCAC+TGG - chr2.4:76621532-76621551 None:intergenic 55.0%
ACTGGCGCAAAGGCTCTCAA+TGG + chr2.4:76621187-76621206 MS.gene072101:CDS 55.0%
AGCAAGGGCTCCAATGCACT+GGG - chr2.4:76621531-76621550 None:intergenic 55.0%
AGGGGGATGCATACCTGACT+TGG - chr2.4:76620714-76620733 None:intergenic 55.0%
ATATCCTCCTCTTCGGAGGG+GGG - chr2.4:76620414-76620433 None:intergenic 55.0%
ATGCGTGGAGGAGGAGAAGA+AGG + chr2.4:76620057-76620076 MS.gene072101:CDS 55.0%
CAACAAGGGTAAAGGTCCCC+TGG - chr2.4:76620574-76620593 None:intergenic 55.0%
CATATCCTCCTCTTCGGAGG+GGG - chr2.4:76620415-76620434 None:intergenic 55.0%
CTCTCCCAGAGTGGCAACAA+GGG - chr2.4:76620588-76620607 None:intergenic 55.0%
GAACAGTCACATGGGTCGAC+TGG + chr2.4:76621361-76621380 MS.gene072101:CDS 55.0%
GGGGGATGCATACCTGACTT+GGG - chr2.4:76620713-76620732 None:intergenic 55.0%
GTACACGAGCGTAGGGATAG+TGG - chr2.4:76620527-76620546 None:intergenic 55.0%
GTCAATGCTCGGTCCTCATC+CGG + chr2.4:76621383-76621402 MS.gene072101:CDS 55.0%
TCTCTCCCAGAGTGGCAACA+AGG - chr2.4:76620589-76620608 None:intergenic 55.0%
! TGGGTCGACTGGTCAATGCT+CGG + chr2.4:76621372-76621391 MS.gene072101:CDS 55.0%
!! CAGCTTAAGCACTGGCGCAA+AGG + chr2.4:76621177-76621196 MS.gene072101:CDS 55.0%
!! GTTGGTGGTCAGAGAAGGTG+TGG - chr2.4:76620470-76620489 None:intergenic 55.0%
AGGGTAAAGGTCCCCTGGTG+AGG - chr2.4:76620569-76620588 None:intergenic 60.0%
GATCCTTCCCGTTATGGCCG+AGG + chr2.4:76621123-76621142 MS.gene072101:CDS 60.0%
GGTCCTCATCCGGCAACCTA+CGG + chr2.4:76621393-76621412 MS.gene072101:CDS 60.0%
GTCCTCATCCGGCAACCTAC+GGG + chr2.4:76621394-76621413 MS.gene072101:CDS 60.0%
TAGTAGCCGCCTCTCCGCAT+CGG - chr2.4:76620971-76620990 None:intergenic 60.0%
TATTGCGGCTGTAGCGGCCT+CGG - chr2.4:76621143-76621162 None:intergenic 60.0%
TCATCCGGCAACCTACGGGA+GGG + chr2.4:76621398-76621417 MS.gene072101:CDS 60.0%
TGTAGCGGCCTCGGCCATAA+CGG - chr2.4:76621134-76621153 None:intergenic 60.0%
TTACTCTGGCCGATGCGGAG+AGG + chr2.4:76620959-76620978 MS.gene072101:CDS 60.0%
TTGTCCCTCCCGTAGGTTGC+CGG - chr2.4:76621405-76621424 None:intergenic 60.0%
CTCATCCGGCAACCTACGGG+AGG + chr2.4:76621397-76621416 MS.gene072101:CDS 65.0%
CTCCCGTAGGTTGCCGGATG+AGG - chr2.4:76621399-76621418 None:intergenic 65.0%
GGAGGAGGAGGAGGAGAAGG+AGG - chr2.4:76620378-76620397 None:intergenic 65.0%
GGAGGAGGAGGAGGAGGAGA+AGG - chr2.4:76620381-76620400 None:intergenic 65.0%
GTAGCGGCCTCGGCCATAAC+GGG - chr2.4:76621133-76621152 None:intergenic 65.0%
ACCACCCCCCTCCGAAGAGG+AGG + chr2.4:76620407-76620426 MS.gene072101:CDS 70.0%
CTCTGGCCGATGCGGAGAGG+CGG + chr2.4:76620962-76620981 MS.gene072101:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG - chr2.4:76620384-76620403 None:intergenic 70.0%
GGTGGAGGAGGAGGAGGAGG+AGG - chr2.4:76620390-76620409 None:intergenic 70.0%
TCCACCACCCCCCTCCGAAG+AGG + chr2.4:76620404-76620423 MS.gene072101:CDS 70.0%
TCCTCCTCTTCGGAGGGGGG+TGG - chr2.4:76620411-76620430 None:intergenic 70.0%
TCCTCTTCGGAGGGGGGTGG+TGG - chr2.4:76620408-76620427 None:intergenic 70.0%
! CGGCCTCGGCCATAACGGGA+AGG - chr2.4:76621129-76621148 None:intergenic 70.0%
! TCTTCGGAGGGGGGTGGTGG+AGG - chr2.4:76620405-76620424 None:intergenic 70.0%
!! GGTGGTGGAGGAGGAGGAGG+AGG - chr2.4:76620393-76620412 None:intergenic 70.0%
!! GAGGGGGGTGGTGGAGGAGG+AGG - chr2.4:76620399-76620418 None:intergenic 75.0%
!! GGGGGTGGTGGAGGAGGAGG+AGG - chr2.4:76620396-76620415 None:intergenic 75.0%
!! TCGGAGGGGGGTGGTGGAGG+AGG - chr2.4:76620402-76620421 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 76620057 76621581 76620057 ID=MS.gene072101
chr2.4 mRNA 76620057 76621581 76620057 ID=MS.gene072101.t1;Parent=MS.gene072101
chr2.4 exon 76620057 76620719 76620057 ID=MS.gene072101.t1.exon1;Parent=MS.gene072101.t1
chr2.4 CDS 76620057 76620719 76620057 ID=cds.MS.gene072101.t1;Parent=MS.gene072101.t1
chr2.4 exon 76620940 76621581 76620940 ID=MS.gene072101.t1.exon2;Parent=MS.gene072101.t1
chr2.4 CDS 76620940 76621581 76620940 ID=cds.MS.gene072101.t1;Parent=MS.gene072101.t1
Gene Sequence

>MS.gene072101

ATGCGTGGAGGAGGAGAAGAAGGAGACAGAGATCCGACGAAGCAACAGCAACAGCACTCTGGTTTGGTTAGATTGGTACAACAACTTGTTACATTGTTGCTTGTTTTTGCTGCTGGTGTCGTCATTGGTTTAACCACAACTTCTCACATTCACTACTTCAATACAAGTCACAACAATAATAACTATCTTCATTTCATTTCCCAACAACCTTCTTCTTCTTCTGATGTCTTCAATCTCGATACTACTATTCACACCCTCCAACAACAACCCAACAACAACAACAACAACAACTGTAACTGTAATTGTACAATCATTGAACCTCCTTCTCCTCCTCCTCCTCCTCCTCCTCCACCACCCCCCTCCGAAGAGGAGGATATGTTGGAGTCCTTTCTTCATCCTTCCAACATCACCCACACCTTCTCTGACCACCAACTTTTCTGGAGGGCTTCCTTGGTTCCTAAGAAATACCACTATCCCTACGCTCGTGTACCTAAACTCGCTTTCATGTACCTCACCAGGGGACCTTTACCCTTGTTGCCACTCTGGGAGAGATTCTTTCAAGGCCACTCCCATCTCTTCAACATTTATATCCACGCTCCTCCTGGTTATATTCTCAACGTTTCTGATTCTTCTCCCTTTTATCGACGCAATATCCCAAGTCAGGTATGCATCCCCCTTAATTCTATCAAGGAAACTTCTTTGCTTTTATTTAAATGAATTGTTTGTACTTGTAGGGCTGTAGTATTCACCATGTATGAATGAATCATAAACCATCCATCTCTTTACTCATATTTTCACTCCCACTAAATCATCAAAACTATAATATTAATTTATGTTATTTGTAGAGAGTATTCTATTCTTATGAGTTTTGTCTTATTCACAGGCTGTTTCTTGGGGAACAGTTACTCTGGCCGATGCGGAGAGGCGGCTACTAGCCAATGCACTGCTCGACTTTTCAAATGAGCGCTTTGTTCTACTCTCAGAGAGTTGCATCCCAGTCTACAACTTCCCTACTGTTTACAGATATCTCATTGATTCTGCACACAGCTTTGTTGAGTCATATGATGATCCTTCCCGTTATGGCCGAGGCCGCTACAGCCGCAATATGCTTCCTGATATTCAGCTTAAGCACTGGCGCAAAGGCTCTCAATGGTTCGAACTCAACCGTGCACTTGCTGTTTATATAGTCTCTGACACCCAATACTTTTCCCTCTTCCGTAAATACTGTAAGCCTGCTTGCTACCCAGATGAACACTACCTTCCAACCTTCCTGAATATGTTCCATGGTTCCCTTAATTCAAACAGAACAGTCACATGGGTCGACTGGTCAATGCTCGGTCCTCATCCGGCAACCTACGGGAGGGACAATATAACTGTCGGTTTTATACAGGCTATAAGGAACAATGGATCCCTTTGTCCATATAATTCAGATATGACTTCCATTTGTTACCTCTTTGCTCGCAAGTTTGATCCCAGTGCATTGGAGCCCTTGCTTGACCTCTCTTCCGAAGTCATGAACTTTTAA
Protein sequence

>MS.gene072101.t1

MRGGGEEGDRDPTKQQQQHSGLVRLVQQLVTLLLVFAAGVVIGLTTTSHIHYFNTSHNNNNYLHFISQQPSSSSDVFNLDTTIHTLQQQPNNNNNNNCNCNCTIIEPPSPPPPPPPPPPPSEEEDMLESFLHPSNITHTFSDHQLFWRASLVPKKYHYPYARVPKLAFMYLTRGPLPLLPLWERFFQGHSHLFNIYIHAPPGYILNVSDSSPFYRRNIPSQAVSWGTVTLADAERRLLANALLDFSNERFVLLSESCIPVYNFPTVYRYLIDSAHSFVESYDDPSRYGRGRYSRNMLPDIQLKHWRKGSQWFELNRALAVYIVSDTQYFSLFRKYCKPACYPDEHYLPTFLNMFHGSLNSNRTVTWVDWSMLGPHPATYGRDNITVGFIQAIRNNGSLCPYNSDMTSICYLFARKFDPSALEPLLDLSSEVMNF